MD12G1188600.v1.1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009915
Physical Location & Seq
Reverse (-)
27038749 .. 27042511
3763 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1188600.v1.1.491

Sequence Viewer

Length: 2898 bp
ATGGCGTTCGAGCTCTGTTTTCTCCTTCTTCTTGCTCCTCTTCTGGTACTCGCCTTAGACCCCGCCTTTAATGACGACGTTTTGGGGCTGATCGTCTTCAAGGCCGGGCTTTCTGACCCGGAAGCGAAGCTTGCTTCGTGGAGCGAGGACGACGGCAGTCCCTGCAACTGGGCAGGCGTCAAATGCGACCCGACAACCGGCAGAGTCGCCGAGGTTGTTCTCGACGGCTTTTCGCTGTCCGGGCACATCGGCCGCGGCCTTCTGCGGCTGCAGAACCTTAAAAAACTCTCGCTTTCCGACAACAACTTCACTGGGTTCATAAACCCTGATCTCCCACACCTCGGCAGCTTGCAGGTTATCGATTTGAGCCGGAACAGCCTATCTGGGTCGATCCCGGAGGAGTTCTTCAAGCAATGTGGATCCCTTGGGGTGGTTTCTTTCGCCGGAAACAACCTCACGGGGCGGATTCCGGAGTCCCTGAGCTTGTGCCAGACCTTGGTGGAGGTGAACTTTTCATCGAATTGGCTGTCTGGGAAATTGCCTCCTGGGATTTGGTACTTGAGAATGCTTCAATCAATCGACTTGTCGGATAACTTGCTGGAGGGGGAAGTTCCTAAAGGTATGGAGAATTTGTATGATTTGAGAGTGATCAACTTGGGGAAAAACCGGTTTTCAGGACAGCTTCCAGGGAACATTGGAAGCTGTTTGAAGCTGAAGTTGCTTGATTTTAGTGGTAATTTGTTTTCTGGGAGAATCCCGGATTCAATTCGGAGTCTCGATTCATGTACATCTCTGAGTTTACAGGGGAATTTTCTTGCGGGGCAAGTTCCGAATTGGCTTGGTGATTTGAAAAACCTTGTGATGTTAGATGTGTCTGGAAATAATTTATCTGGTGAAATTCCAAGTTCGTTAGGAAATCTAGAGGTGCTCGAAAAGTTGAATTTGTCTAGGAATGGATTTACTGGAAGCTTGCCTGATGCATTGGCAAACTGCATCAATCTGTTAGCTATAGATGTTAGCCATAATCTGTTGGCAGGTAAGCTTCCTTTGTGGATTTTTAAGCTCGGTCTGCGCAGTGTTTTGCTTTCTGGCAACCGATTAGGTGGAAGTGACGAGTACAGTTCACTGGCGTCAATGGCAGCGTCGAATGGAGGCCTGCAGGTCTTGGATTTGTCTTCGAACGCATTTTCTGATGTGCTTCCATCGGACATTGGTGTTCTTAGTAGCTTGCAGTTCTTGAATATGTCCGGGAACCATCTGTTGGGTTGGATTCCGGCAAGTATAGGTGAGTTAAGGGCGGCTTATGTTCTCGACTTGAGTGACAATTGGCTAAATGGAAGCATTCCTGATGAAATCGGAGGGGCAGTCTCTCTCAAGGAACTAAGGCTGCAGAAGAACTTTCTAACCGGGAAAGTTCCAGCCGAAATTGTGAAGTGCTCATCTCTGACAAATCTGATTTTATCTCAGAACAATCTGACTGGCCCTATTCCTACTGCCATTGCTAACCTTACCAATCTTGAATATGTGGACTTGTCTTTGAACAAATTCTCAGGAGGCATACCGAAAGAGCTGACAAATCTTTCCCATCTCCTCTACTTCAATGTCTCCCACAACCACCTAGAGGGTGAACTACCACTAGGGGGATTCTTCAACACTATCCCACCTTCATCTGTCTTGGGCAATCCATCTCTTTGTGGATCTGCTGTTAGCCGTGCCTGCCCTTCCGTCCACCCCAAACCCATTGTCCTCAACCCTAATTCTTCCAATCCCGTCGGTGGTTCTTCCTCTCCAACTCATGGTCACAAGATCGTATTCAGCATATCAGCCCTCATTGCTATAGGCGCAGCTGTATTTATTGCCATTGGTGTCATAGCTGTAACCATCCTCAACATGCATGCACAGTCTTCATTATCACGTTCTGCTGCTCCTCTTGAGTTATCAGGTGGGGAAGATTATAGTTGTTCCCCGACTAATGATCCAAACTACGGCAAGCTTGTCATGTTTTCTGGTGATGCTGACTTTGCTGCTGGGAGACAAGCCTTGCTCAACAAGGACTGTGAACTTGGTCGTGGTGGGTTTGGAGTAGTTTACAAAACAGCCCTTTGTGATGGGCGTTCTGTTGCGATAAAAATGCTATCAGTCTCAGGTTTGATCAAGTCCCAAGAAGATTTCGAGAGGGAAGTTAAGGGACTTGGAAAGATCAGGCACCGTAATCTTGTGGCACTTGAAGGGTATTACTGGACTCCGTCCTTGCAGCTCATCATTTATGAGTACATACCATGCGGGAGTCTATTTAAGAATCTGCATGACGGACCTGGCAAAACCTGTCTCACTTGGCGGCAGAGGTTCCACATAATTCTCGGGATGGCAAAGGGCTTGGCTCATCTGCACCAAATGAACATAATCCACTACAATTTGAAATCAACTAATGTTTTGATAGACAACAATGGTGAGCCTAAGGTGGGAGACATTGGCCTGGCAAGGCTGTTGCCAACACTAGACCGGTGCATCTTAAGCAGCAAAATCCAAAGCGCGCTTGGATACATGGCGCCCGAGTTTGCATGTCAAACGGTTAAGATTACTGAGAAATGTGATGTCTATGGCTTCGGGATCTTGGTTTTGGAGGTGGTTACTGGAAAGAGGCCTGTCGAATATGTGGAGGATGATGTGATAGTACTCTCTGATATGGTGAGGGGAGCGCTGGGGGAAGGCCGGGTGGAGGAATGTCTGGACAAAAACCTCCTTGGCAACTTTCCAGCAGAGGAGGCAATTCCGGTGATCAAGCTGGGTTTGATATGCGCGTCTCAAGTGCCATCAAATCGCCCAGACATGGGTGAAGTGATCAACATTTTAGAACTCATCCAATGCCCTTCAGAAGGCCAGGAGGAATTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

966

Amino Acids

103.51

Weight (kDa)

5.33

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 63 1.8e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 188 - 335 7.5e-09 Leucine-rich repeat region
LRR_8 PF13855 263 - 319 7.5e-06 Leucine rich repeat
LRR_8 PF13855 481 - 540 3e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 684 - 944 4.2e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 685 - 891 1.4e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1073
Acc36I ACCTGC 3 cut(s) 343, 1025, 1150
AccB1I GGYRCC 2 cut(s) 2205, 2548
AccB7I CCANNNNNTGG 3 cut(s) 496, 1261, 1796
AccII CGCG 3 cut(s) 255, 2534, 2801
AccIII TCCGGA 1 cut(s) 469
AciI CCGC 9 cut(s) 63, 253, 255, 265, 463, 818, 1298, 2283, 2338
AclWI GGATC 6 cut(s) 385, 414, 427, 1705, 1970, 2618
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 5 cut(s) 628, 808, 897, 940, 1544
AcuI CTGAAG 2 cut(s) 734, 2856
AcyI GRCGYC 3 cut(s) 177, 1130, 2549
AfaI GTAC 6 cut(s) 48, 557, 787, 1118, 2273, 2676
AfeI AGCGCT 1 cut(s) 2700
AflII CTTAAG 1 cut(s) 2512
AgeI ACCGGT 2 cut(s) 666, 2502
AjnI CCWGG 5 cut(s) 544, 685, 2314, 2475, 2880
AjuI GAANNNNNNNTTGG 2 cut(s) 1244, 1276
Alw21I GWGCWC 3 cut(s) 15, 930, 1439
Alw26I GTCTC 8 cut(s) 779, 1372, 1609, 2026, 2146, 2333, 2460, 2808
AlwI GGATC 6 cut(s) 385, 414, 427, 1705, 1970, 2618
AlwNI CAGNNNCTG 1 cut(s) 162
Ama87I CYCGRG 2 cut(s) 2360, 2552
Aor13HI TCCGGA 1 cut(s) 469
Aor51HI AGCGCT 1 cut(s) 2700
AoxI GGCC 9 cut(s) 102, 250, 256, 1153, 1480, 2473, 2642, 2710, 2878
ApeKI GCWGC 9 cut(s) 268, 345, 1139, 1387, 1844, 1922, 2024, 2254, 2517
ApoI RAATTY 5 cut(s) 628, 808, 897, 940, 1544
ArsI GACNNNNNNTTYG 4 cut(s) 512, 544, 1728, 1760
AsiGI ACCGGT 2 cut(s) 666, 2502
Asp700I GAANNNNTTC 1 cut(s) 1544
AspLEI GCGC 7 cut(s) 1074, 1844, 2534, 2536, 2551, 2701, 2801
AspS9I GGNCC 2 cut(s) 1481, 2312
AsuC2I CCSGG 8 cut(s) 106, 119, 241, 395, 758, 1249, 1408, 2714
AsuII TTCGAA 1 cut(s) 1178
AvaI CYCGRG 2 cut(s) 2360, 2552
AvaII GGWCC 1 cut(s) 2312
AxyI CCTNAGG 1 cut(s) 2457
BaeGI GKGCMC 1 cut(s) 246
BamHI GGATCC 1 cut(s) 419
BanI GGYRCC 2 cut(s) 2205, 2548
BanII GRGCYC 1 cut(s) 15
BbsI GAAGAC 3 cut(s) 88, 1167, 1896
Bbv12I GWGCWC 3 cut(s) 15, 930, 1439
BbvI GCAGC 9 cut(s) 255, 357, 1151, 1374, 1856, 1909, 2011, 2266, 2529
BccI CCATC 8 cut(s) 1210, 1263, 1593, 1693, 1889, 2102, 2359, 2821
BceAI ACGGC 4 cut(s) 169, 241, 1695, 2002
BcgI CGANNNNNNTGC 2 cut(s) 2113, 2147
BciT130I CCWGG 5 cut(s) 546, 687, 2316, 2477, 2882
BciVI GTATCC 1 cut(s) 2534
BclI TGATCA 4 cut(s) 648, 2151, 2778, 2841
BcnI CCSGG 8 cut(s) 106, 119, 241, 395, 758, 1249, 1408, 2714
BcoDI GTCTC 8 cut(s) 779, 1372, 1609, 2026, 2146, 2333, 2460, 2808
BfaI CTAG 5 cut(s) 920, 948, 1619, 1637, 2498
BfmI CTRYAG 5 cut(s) 269, 1008, 1157, 1388, 1836
BfoI RGCGCY 2 cut(s) 2552, 2702
BfrI CTTAAG 1 cut(s) 2512
BfuAI ACCTGC 3 cut(s) 343, 1025, 1150
BfuI GTATCC 1 cut(s) 2534
BmcAI AGTACT 1 cut(s) 2676
Bme18I GGWCC 1 cut(s) 2312
BmeT110I CYCGRG 2 cut(s) 2360, 2552
BmgT120I GGNCC 2 cut(s) 1481, 2312
BmiI GGNNCC 5 cut(s) 421, 1253, 2207, 2348, 2550
BmrI ACTGGG 2 cut(s) 178, 321
BmsI GCATC 4 cut(s) 967, 1002, 2002, 2517
BmuI ACTGGG 2 cut(s) 178, 321
BoxI GACNNNNGTC 1 cut(s) 156
BpiI GAAGAC 3 cut(s) 88, 1167, 1896
BpmI CTGGAG 1 cut(s) 620
Bpu10I CCTNAGC 1 cut(s) 479
Bpu14I TTCGAA 1 cut(s) 1178
BpuEI CTTGAG 5 cut(s) 580, 1336, 1358, 1952, 2790
BpuMI CCSGG 8 cut(s) 106, 119, 241, 395, 758, 1249, 1408, 2714
Bsa29I ATCGAT 1 cut(s) 360
BsaHI GRCGYC 3 cut(s) 177, 1130, 2549
BsaJI CCNNGG 8 cut(s) 210, 253, 340, 424, 495, 545, 686, 2743
BsaWI WCCGGW 4 cut(s) 469, 666, 2502, 2773
BsaXI ACNNNNNCTCC 4 cut(s) 617, 647, 1350, 1380
Bse118I RCCGGY 3 cut(s) 197, 666, 2502
Bse1I ACTGG 7 cut(s) 173, 316, 967, 1131, 1483, 2243, 2638
Bse21I CCTNAGG 1 cut(s) 2457
Bse3DI GCAATG 3 cut(s) 419, 1497, 1830
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 5 cut(s) 546, 687, 2316, 2477, 2882
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 8 cut(s) 210, 253, 340, 424, 495, 545, 686, 2743
BseGI GGATG 4 cut(s) 1881, 2370, 2668, 2859
BseMI GCAATG 3 cut(s) 419, 1497, 1830
BseMII CTCAG 6 cut(s) 470, 785, 1478, 1563, 2157, 2574
BseNI ACTGG 7 cut(s) 173, 316, 967, 1131, 1483, 2243, 2638
BsePI GCGCGC 1 cut(s) 2532
BseRI GAGGAG 5 cut(s) 27, 413, 1580, 1917, 2777
BseSI GKGCMC 1 cut(s) 246
BseX3I CGGCCG 1 cut(s) 250
BseXI GCAGC 9 cut(s) 255, 357, 1151, 1374, 1856, 1909, 2011, 2266, 2529
BseYI CCCAGC 3 cut(s) 2027, 2701, 2785
BsgI GTGCAG 1 cut(s) 2372
Bsh1236I CGCG 3 cut(s) 255, 2534, 2801
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 9 cut(s) 104, 252, 258, 1155, 1482, 2475, 2644, 2712, 2880
BshNI GGYRCC 2 cut(s) 2205, 2548
BshTI ACCGGT 2 cut(s) 666, 2502
BshVI ATCGAT 1 cut(s) 360
BsiEI CGRYCG 1 cut(s) 253
BsiHKAI GWGCWC 3 cut(s) 15, 930, 1439
BsiHKCI CYCGRG 2 cut(s) 2360, 2552
BslFI GGGAC 4 cut(s) 144, 460, 2143, 2202
BsmAI GTCTC 8 cut(s) 779, 1372, 1609, 2026, 2146, 2333, 2460, 2808
BsmBI CGTCTC 1 cut(s) 2808
BsmFI GGGAC 4 cut(s) 144, 460, 2143, 2202
BsmI GAATGC 2 cut(s) 570, 1341
BsnI GGCC 9 cut(s) 104, 252, 258, 1155, 1482, 2475, 2644, 2712, 2880
BsoBI CYCGRG 2 cut(s) 2360, 2552
Bsp119I TTCGAA 1 cut(s) 1178
Bsp1286I GDGCHC 4 cut(s) 15, 246, 930, 1439
Bsp13I TCCGGA 1 cut(s) 469
Bsp1407I TGTACA 1 cut(s) 785
BspACI CCGC 9 cut(s) 63, 253, 255, 265, 463, 818, 1298, 2283, 2338
BspANI GGCC 9 cut(s) 104, 252, 258, 1155, 1482, 2475, 2644, 2712, 2880
BspCNI CTCAG 6 cut(s) 471, 786, 1477, 1562, 2156, 2575
BspDI ATCGAT 1 cut(s) 360
BspEI TCCGGA 1 cut(s) 469
BspFNI CGCG 3 cut(s) 255, 2534, 2801
BspLI GGNNCC 5 cut(s) 421, 1253, 2207, 2348, 2550
BspMAI CTGCAG 3 cut(s) 273, 1161, 1392
BspMI ACCTGC 3 cut(s) 343, 1025, 1150
BspPI GGATC 6 cut(s) 385, 414, 427, 1705, 1970, 2618
BspT104I TTCGAA 1 cut(s) 1178
BspT107I GGYRCC 2 cut(s) 2205, 2548
BspTI CTTAAG 1 cut(s) 2512
BsrDI GCAATG 3 cut(s) 419, 1497, 1830
BsrFI RCCGGY 3 cut(s) 197, 666, 2502
BsrGI TGTACA 1 cut(s) 785
BsrI ACTGG 7 cut(s) 173, 316, 967, 1131, 1483, 2243, 2638
BssAI RCCGGY 3 cut(s) 197, 666, 2502
BssECI CCNNGG 8 cut(s) 210, 253, 340, 424, 495, 545, 686, 2743
BssHII GCGCGC 1 cut(s) 2532
BssNI GRCGYC 3 cut(s) 177, 1130, 2549
BssT1I CCWWGG 3 cut(s) 424, 495, 2743
Bst2UI CCWGG 5 cut(s) 546, 687, 2316, 2477, 2882
Bst4CI ACNGT 5 cut(s) 1121, 1902, 2057, 2210, 2572
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 3 cut(s) 177, 1130, 2549
BstAFI CTTAAG 1 cut(s) 2512
BstAPI GCANNNNNTGC 1 cut(s) 162
BstAUI TGTACA 1 cut(s) 785
BstBI TTCGAA 1 cut(s) 1178
BstDSI CCRYGG 1 cut(s) 253
BstENI CCTNNNNNAGG 1 cut(s) 2874
BstF5I GGATG 4 cut(s) 1881, 2370, 2668, 2859
BstFNI CGCG 3 cut(s) 255, 2534, 2801
BstH2I RGCGCY 2 cut(s) 2552, 2702
BstHHI GCGC 7 cut(s) 1074, 1844, 2534, 2536, 2551, 2701, 2801
BstMAI GTCTC 8 cut(s) 779, 1372, 1609, 2026, 2146, 2333, 2460, 2808
BstMCI CGRYCG 1 cut(s) 253
BstNI CCWGG 5 cut(s) 546, 687, 2316, 2477, 2882
BstNSI RCATGY 3 cut(s) 1894, 1898, 2565
BstPAI GACNNNNGTC 1 cut(s) 156
BstSFI CTRYAG 5 cut(s) 269, 1008, 1157, 1388, 1836
BstSLI GKGCMC 1 cut(s) 246
BstUI CGCG 3 cut(s) 255, 2534, 2801
BstV1I GCAGC 9 cut(s) 255, 357, 1151, 1374, 1856, 1909, 2011, 2266, 2529
BstV2I GAAGAC 3 cut(s) 88, 1167, 1896
BstX2I RGATCY 3 cut(s) 419, 1697, 2610
BstYI RGATCY 3 cut(s) 419, 1697, 2610
BstZI CGGCCG 1 cut(s) 250
Bsu15I ATCGAT 1 cut(s) 360
Bsu36I CCTNAGG 1 cut(s) 2457
BsuI GTATCC 1 cut(s) 2534
BsuRI GGCC 9 cut(s) 104, 252, 258, 1155, 1482, 2475, 2644, 2712, 2880
BsuTUI ATCGAT 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 4 cut(s) 1881, 2370, 2668, 2859
BtsI GCAGTG 1 cut(s) 1081
BtsIMutI CAGTG 3 cut(s) 309, 1081, 1124
BveI ACCTGC 3 cut(s) 343, 1025, 1150
CaiI CAGNNNCTG 1 cut(s) 162
CfoI GCGC 7 cut(s) 1074, 1844, 2534, 2536, 2551, 2701, 2801
Cfr10I RCCGGY 3 cut(s) 197, 666, 2502
Cfr13I GGNCC 2 cut(s) 1481, 2312
Cfr42I CCGCGG 1 cut(s) 256
ClaI ATCGAT 1 cut(s) 360
CseI GACGC 4 cut(s) 166, 1119, 1131, 2790
Csp6I GTAC 6 cut(s) 47, 556, 786, 1117, 2272, 2675
CspAI ACCGGT 2 cut(s) 666, 2502
CspCI CAANNNNNGTGG 2 cut(s) 397, 432
CviQI GTAC 6 cut(s) 47, 556, 786, 1117, 2272, 2675
DinI GGCGCC 1 cut(s) 2550
EaeI YGGCCR 1 cut(s) 250
EagI CGGCCG 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
EciI GGCGGA 1 cut(s) 478
Ecl136II GAGCTC 1 cut(s) 13
EclXI CGGCCG 1 cut(s) 250
Eco130I CCWWGG 3 cut(s) 424, 495, 2743
Eco147I AGGCCT 2 cut(s) 1155, 2644
Eco24I GRGCYC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 2312
Eco47III AGCGCT 1 cut(s) 2700
Eco52I CGGCCG 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 13
Eco57I CTGAAG 2 cut(s) 734, 2856
Eco81I CCTNAGG 1 cut(s) 2457
Eco88I CYCGRG 2 cut(s) 2360, 2552
EcoICRI GAGCTC 1 cut(s) 13
EcoNI CCTNNNNNAGG 1 cut(s) 2874
EcoRII CCWGG 5 cut(s) 544, 685, 2314, 2475, 2880
EcoT14I CCWWGG 3 cut(s) 424, 495, 2743
EcoT22I ATGCAT 2 cut(s) 982, 1896
EcoT38I GRGCYC 1 cut(s) 15
EgeI GGCGCC 1 cut(s) 2550
EheI GGCGCC 1 cut(s) 2550
ErhI CCWWGG 3 cut(s) 424, 495, 2743
Esp3I CGTCTC 1 cut(s) 2808
FalI AAGNNNNNCTT 4 cut(s) 114, 146, 1285, 1317
FaqI GGGAC 4 cut(s) 144, 460, 2143, 2202
FauI CCCGC 3 cut(s) 70, 811, 2276
FbaI TGATCA 4 cut(s) 648, 2151, 2778, 2841
FokI GGATG 4 cut(s) 1868, 2377, 2675, 2846
FriOI GRGCYC 1 cut(s) 15
FspBI CTAG 5 cut(s) 920, 948, 1619, 1637, 2498
FspI TGCGCA 1 cut(s) 1073
GlaI GCGC 7 cut(s) 1073, 1843, 2533, 2535, 2550, 2700, 2800
GsaI CCCAGC 3 cut(s) 2031, 2705, 2789
GsuI CTGGAG 1 cut(s) 620
HaeII RGCGCY 2 cut(s) 2552, 2702
HaeIII GGCC 9 cut(s) 104, 252, 258, 1155, 1482, 2475, 2644, 2712, 2880
HgaI GACGC 4 cut(s) 166, 1119, 1131, 2790
HhaI GCGC 7 cut(s) 1074, 1844, 2534, 2536, 2551, 2701, 2801
Hin1I GRCGYC 3 cut(s) 177, 1130, 2549
Hin6I GCGC 7 cut(s) 1072, 1842, 2532, 2534, 2549, 2699, 2799
HinP1I GCGC 7 cut(s) 1072, 1842, 2532, 2534, 2549, 2699, 2799
HindIII AAGCTT 4 cut(s) 128, 967, 1040, 1991
Hpy166II GTNNAC 8 cut(s) 508, 800, 1124, 1528, 1628, 1729, 2060, 2089
Hpy8I GTNNAC 8 cut(s) 508, 800, 1124, 1528, 1628, 1729, 2060, 2089
Hpy99I CGWCG 5 cut(s) 80, 155, 227, 1147, 1775
HpyAV CCTTC 8 cut(s) 35, 269, 1674, 1731, 2222, 2702, 2870, 2880
HpyCH4III ACNGT 5 cut(s) 1121, 1902, 2057, 2210, 2572
HpyCH4IV ACGT 2 cut(s) 78, 1915
HpySE526I ACGT 2 cut(s) 78, 1915
Hsp92I GRCGYC 3 cut(s) 177, 1130, 2549
HspAI GCGC 7 cut(s) 1072, 1842, 2532, 2534, 2549, 2699, 2799
KasI GGCGCC 1 cut(s) 2548
Kpn2I TCCGGA 1 cut(s) 469
Ksp22I TGATCA 4 cut(s) 648, 2151, 2778, 2841
KspI CCGCGG 1 cut(s) 256
LmnI GCTCC 4 cut(s) 40, 141, 1930, 2696
Lsp1109I GCAGC 9 cut(s) 255, 357, 1151, 1374, 1856, 1909, 2011, 2266, 2529
LweI GCATC 4 cut(s) 967, 1002, 2002, 2517
MaeI CTAG 5 cut(s) 920, 948, 1619, 1637, 2498
MaeII ACGT 2 cut(s) 78, 1915
MaeIII GTNAC 5 cut(s) 1109, 1319, 1799, 1876, 2629
MfeI CAATTG 1 cut(s) 1324
MflI RGATCY 3 cut(s) 419, 1697, 2610
MhlI GDGCHC 4 cut(s) 15, 246, 930, 1439
Mly113I GGCGCC 1 cut(s) 2549
MlyI GAGTC 5 cut(s) 213, 482, 781, 2236, 2296
MmeI TCCRAC 4 cut(s) 321, 567, 1247, 1814
Mph1103I ATGCAT 2 cut(s) 982, 1896
MroI TCCGGA 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 1544
MseI TTAA 8 cut(s) 69, 279, 1059, 1292, 2184, 2295, 2513, 2574
MspA1I CMGCKG 2 cut(s) 255, 1847
MspCI CTTAAG 1 cut(s) 2512
MunI CAATTG 1 cut(s) 1324
Mva1269I GAATGC 2 cut(s) 570, 1341
MvaI CCWGG 5 cut(s) 546, 687, 2316, 2477, 2882
MvnI CGCG 3 cut(s) 255, 2534, 2801
NarI GGCGCC 1 cut(s) 2549
NciI CCSGG 8 cut(s) 106, 119, 241, 395, 758, 1249, 1408, 2714
NlaIV GGNNCC 5 cut(s) 421, 1253, 2207, 2348, 2550
NmeAIII GCCGAG 2 cut(s) 235, 321
NmuCI GTSAC 3 cut(s) 1109, 1319, 1799
NsbI TGCGCA 1 cut(s) 1073
NsiI ATGCAT 2 cut(s) 982, 1896
NspI RCATGY 3 cut(s) 1894, 1898, 2565
NspV TTCGAA 1 cut(s) 1178
PaeI GCATGC 1 cut(s) 1898
PauI GCGCGC 1 cut(s) 2532
PceI AGGCCT 2 cut(s) 1155, 2644
PctI GAATGC 2 cut(s) 570, 1341
PdmI GAANNNNTTC 1 cut(s) 1544
PfeI GAWTC 7 cut(s) 466, 753, 761, 779, 1270, 1644, 2299
PflFI GACNNNGTC 1 cut(s) 2245
PflMI CCANNNNNTGG 3 cut(s) 496, 1261, 1796
PfoI TCCNGGA 3 cut(s) 393, 756, 1247
PinAI ACCGGT 2 cut(s) 666, 2502
PleI GAGTC 5 cut(s) 212, 481, 780, 2236, 2295
PluTI GGCGCC 1 cut(s) 2552
PpsI GAGTC 5 cut(s) 212, 481, 780, 2236, 2295
PshAI GACNNNNGTC 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 15
Psp6I CCWGG 5 cut(s) 544, 685, 2314, 2475, 2880
PspFI CCCAGC 3 cut(s) 2027, 2701, 2785
PspGI CCWGG 5 cut(s) 544, 685, 2314, 2475, 2880
PspN4I GGNNCC 5 cut(s) 421, 1253, 2207, 2348, 2550
PspPI GGNCC 2 cut(s) 1481, 2312
PstI CTGCAG 3 cut(s) 273, 1161, 1392
PstNI CAGNNNCTG 1 cut(s) 162
PsuI RGATCY 3 cut(s) 419, 1697, 2610
PsyI GACNNNGTC 1 cut(s) 2245
PteI GCGCGC 1 cut(s) 2532
PvuII CAGCTG 1 cut(s) 1847
RsaI GTAC 6 cut(s) 48, 557, 787, 1118, 2273, 2676
RsaNI GTAC 6 cut(s) 47, 556, 786, 1117, 2272, 2675
SacI GAGCTC 1 cut(s) 15
SacII CCGCGG 1 cut(s) 256
SaqAI TTAA 8 cut(s) 69, 279, 1059, 1292, 2184, 2295, 2513, 2574
Sau96I GGNCC 2 cut(s) 1481, 2312
SbfI CCTGCAGG 1 cut(s) 1161
ScaI AGTACT 1 cut(s) 2676
SchI GAGTC 5 cut(s) 213, 482, 781, 2236, 2296
SdaI CCTGCAGG 1 cut(s) 1161
SduI GDGCHC 4 cut(s) 15, 246, 930, 1439
SfaNI GCATC 4 cut(s) 967, 1002, 2002, 2517
SfcI CTRYAG 5 cut(s) 269, 1008, 1157, 1388, 1836
SfoI GGCGCC 1 cut(s) 2550
Sfr303I CCGCGG 1 cut(s) 256
SfuI TTCGAA 1 cut(s) 1178
SgrBI CCGCGG 1 cut(s) 256
SinI GGWCC 1 cut(s) 2312
SmlI CTYRAG 6 cut(s) 559, 1315, 1373, 1931, 2512, 2805
SmoI CTYRAG 6 cut(s) 559, 1315, 1373, 1931, 2512, 2805
SphI GCATGC 1 cut(s) 1898
Sse8387I CCTGCAGG 1 cut(s) 1161
SseBI AGGCCT 2 cut(s) 1155, 2644
SsiI CCGC 9 cut(s) 63, 253, 255, 265, 463, 818, 1298, 2283, 2338
SspDI GGCGCC 1 cut(s) 2548
SspMI CTAG 5 cut(s) 920, 948, 1619, 1637, 2498
SstI GAGCTC 1 cut(s) 15
StuI AGGCCT 2 cut(s) 1155, 2644
StyI CCWWGG 3 cut(s) 424, 495, 2743
TaaI ACNGT 5 cut(s) 1121, 1902, 2057, 2210, 2572
TaiI ACGT 2 cut(s) 81, 1918
TaqII GACCGA 1 cut(s) 1055
TatI WGTACW 4 cut(s) 785, 1116, 2271, 2674
TauI GCSGC 5 cut(s) 255, 258, 268, 1301, 2341
TfiI GAWTC 7 cut(s) 466, 753, 761, 779, 1270, 1644, 2299
Tru1I TTAA 8 cut(s) 69, 279, 1059, 1292, 2184, 2295, 2513, 2574
Tru9I TTAA 8 cut(s) 69, 279, 1059, 1292, 2184, 2295, 2513, 2574
TscAI CASTG 3 cut(s) 316, 1081, 1131
TseFI GTSAC 3 cut(s) 1109, 1319, 1799
TseI GCWGC 9 cut(s) 268, 345, 1139, 1387, 1844, 1922, 2024, 2254, 2517
Tsp45I GTSAC 3 cut(s) 1109, 1319, 1799
TspDTI ATGAA 7 cut(s) 307, 504, 771, 1365, 1656, 1896, 2411
TspGWI ACGGA 3 cut(s) 1714, 2235, 2325
TspRI CASTG 3 cut(s) 316, 1081, 1131
Tth111I GACNNNGTC 1 cut(s) 2245
Van91I CCANNNNNTGG 3 cut(s) 496, 1261, 1796
Vha464I CTTAAG 1 cut(s) 2512
VpaK11BI GGWCC 1 cut(s) 2312
XagI CCTNNNNNAGG 1 cut(s) 2874
XapI RAATTY 5 cut(s) 628, 808, 897, 940, 1544
XbaI TCTAGA 1 cut(s) 919
XceI RCATGY 3 cut(s) 1894, 1898, 2565
XcmI CCANNNNNNNNNTGG 1 cut(s) 2534
XmnI GAANNNNTTC 1 cut(s) 1544
XspI CTAG 5 cut(s) 920, 948, 1619, 1637, 2498
ZrmI AGTACT 1 cut(s) 2676
Zsp2I ATGCAT 2 cut(s) 982, 1896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.