RchiOBHm_Chr3g0460441
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0009915
Physical Location & Seq
Forward (+)
8555244 .. 8559254
4011 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42695

Sequence Viewer

Length: 2865 bp
ATGTCACTCAAACTCGTCTCTCTCCTTCTTCTCTGGTCACCTGTGTTCGTACTTTCCTTAAACCCTGTTTTCAACGACGACGTTTTGGGGCTGATCGTGTTCAAGGCCGGGCTGCTAGACCCGGAGGCCAAGCTCCGCTCTTGGAACGAAGAGGATGACAGTCCTTGCAATTGGGTCGGTGTCAAATGCGACCCCAGAAGCAACAGAGTCTCGGAGCTTGCCCTCGATGGGTTTGGCCTATCTGGGCATGTAAACCGTGGCCTTTTAAGGCTGCAGTTCATTCAGAGACTCTCACTTTCTAACAACAACTTCACTGGGTCTATAAACCCTGATCTGGCTCACATTGGGACTTTGCAACTCATTGATTTGAGCCAGAATGGCCTCTCCGGGTCGATCCCGGATGAGTTTTTTCAACAATGTGGCTCACTCAGAGTTGTTTCGTTTGCCAAAAACAATCTCTCAGGGAAAATCCCCGAGTCTCTGAGCTTCTGCTCTGCTCTGGTGGCCGTGAACTTTTCCTCGAATCAGCTTTCGGGGAACTTACCATCTGGGATATGGTATTTGAGGGGCCTTCGGGAGCTGGACTTGTCTGCTAACTTGCTCGAGGGTGATGTTCATCAAGGTATTGGATATTTGTATGATTTGAGAGTTGTTAACTTGGGGACAAATCGGTTTTCCGGGTGGCTTCCCCAGGATGTTGGAGGCTGTTCACATTTGAAGTTGCTTGATTTTAGTGATAATTTATTTTCCGGGGGAATTCCTGAGTCGATTAAGAGACTTGGTTTCTGTAGGTCTTTGAGTTTAAAGGGGAACTCTCTTACGGGTCAAGTTCCTGCTTGGATTGGTGAGTTGAGAAGCCTTGGGATGTTAGATTTATCTTGTAATAACTTTTCTGGTGGCATTCCTGGTTCATTAGGAAATCTAAGGCTACTTGAGAAGTTGAATTTATCTATGAATGGATTTACTGGGAGCTTGCCTGAGTCGTTGACAAATTGTTTCAACCTTCTAGCTCTAGATGTTAGCCGAAATCAGTTGGTTGGTAAACTTCCTTCATGGATTTTGAAGCTTGGTGTGGGACGTGGAAGGCTCGAGTACAATCCTTTGAGACCAATAGCTGCCTCTCATGGACGTCTTCAGGTCTTGGATTTGTCTTCCAATGCATTTTCTGATGTGCTTCCATCTGATATTGGGGTTCTTAGTAGCTTGCAGTTCTTGAATGTGTCCCGGAATCAGCTCCTTGGTACTATTCCTGTAAGTATAGGTAATTTGACGTCAGCATATGTTCTGGACTTGAGCAACAATTGGCTGAATGGAAGCATTCCTTCTGAAATTGGAGGAGCAGTCTCACTCAAGGAGCTAAGGCTGCACAAGAACTTTCTAACGGGGAAAGTTCCATCTCAGATTCAGAAGTGCTCATCTCTATCATCGCTGTTGCTATCCCAGAACAATCTTACTGGCCCTGTTCCTGCAGCCATTGCAAACCTTACCAACCTTCAATACGTCGACTTGTCTTTAAACCAATTCTCTGGGAGCTTGCCTAAAGAGTTGACAAATCTTTCCCACCTCCTCTACTTCAATGTATCCTACAATCACCTTCAGGGTGAGTTACCTGTGGGGGGCTTCTTCAACACAATCTCACCCTCATCTATCTCGGGCAATCCGTCCCTATGTGGTTCTGTTCTTAACCTTTCATGCCCTGCTGTTCACCCTAAACCCATTGTCCTCAACCCAAATTCTAACAACACTGGTGGTGGTTCTTCCTCTCTGACTCATGGTCACAAGAATGTATTTAGCATATCTGCTCTCATTGCCATAGGTGCTGCTGCATTCATTGCAATTGGTGTTATAGCTGTTACCGTCCTCAATATGCACGTGCGGTCTTCAATGACACATTCTGCTCCTCTTCCACTACCTGGTGGGGAAGATTTCAGTTGTTCCCCAAGTACTGATTCAAAGTATGGCAAGCTTGTTATGTTTTCTGGTGATGCTGACTTTGCTGCTGGAGCCCAAGCCCTGCTGAACAAGGACTGTGAGCTTGGTCGTGGTGGCTTTGGAGTAGTTTACAGAACGGTCCTCAGAGATGGGCGTTCAGTTGCAATAAAAAAGTTGACAGTCTCAAGTTTGATCAAGTCCCAAGAAGATTTCGAGAGGGAAGTTAAAGGAATTGGAAAGATAAGGCACCATAATCTTGTGACACTTGAAGGGTATTACTGGACTCCTTCCTTGCAGCTGCTCATATATGAATACATACCATGTGGAAGTCTGTATAAGAATCTGCATGATGGACCTGGCCAAAGCGGTCTCTCTTGGCGACAGAGGTTCAACATAATTCTTGGGATGGCAAAGGGTTTGGCTCATCTGCATCGAATGAACTTGATTCACTACAACTTGAAATCAACTAATATTCTGATAGACAGCTCTGGTGAACCAAAAGTAGGAGACTTTAGTCTGGCAAGGCTGTTGCCAATGTTAGACCGGTGCATCTTGAGCAGCAAAATCCAAAGTGCACTTGGGTATATGGCGCCTGAGTTCGCTTGTCAAACTGTCAAGATTACTGAGAAATGTGATGTGTATGGGTACGGGGTCTTGGTTTTGGAGGTGGTGACCGGAAAGCGGCCTGTGGAATATATGGAAGATGATGTGGTGGTGCTCTGTGACATGGTGAGGGGGGCTCTAGAGGAAGGCAGGGTGGAAGAATGTCTTGACAGAAAACTTCTGGGTAATTATCCGGCAGAGGAGGCAATTCCAGTGATCAAGCTTGGTTTGATATGTGCATCACAAGTTCCATCAAATCGTCCTGATATGAGTGAAGTAGTCAACATCTTAGAACTCATCCAATGCCCTTCAGAAGGCCATGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

954

Amino Acids

103.15

Weight (kDa)

6.48

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 64 5.1e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 189 - 337 1.3e-09 Leucine-rich repeat region
LRR_8 PF13855 264 - 320 6.8e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 675 - 941 1e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 676 - 880 4.5e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1132, 1274
AccB1I GGYRCC 2 cut(s) 2178, 2521
AccB7I CCANNNNNTGG 1 cut(s) 1913
AccBSI CCGCTC 1 cut(s) 138
AccI GTMKAC 1 cut(s) 1503
AciI CCGC 4 cut(s) 136, 1876, 2298, 2614
AclWI GGATC 1 cut(s) 388
AcoI YGGCCR 2 cut(s) 504, 2290
AcsI RAATTY 3 cut(s) 756, 943, 1732
AcuI CTGAAG 3 cut(s) 1118, 1580, 2829
AcvI CACGTG 1 cut(s) 1873
AcyI GRCGYC 3 cut(s) 1129, 1271, 2522
AfaI GTAC 5 cut(s) 51, 1094, 1243, 1945, 2579
AfiI CCNNNNNNNGG 7 cut(s) 228, 334, 1616, 1913, 2435, 2613, 2849
AgeI ACCGGT 1 cut(s) 2475
AhdI GACNNNNNGTC 1 cut(s) 1773
AjiI CACGTC 1 cut(s) 1079
AjnI CCWGG 4 cut(s) 690, 904, 1912, 2287
AjuI GAANNNNNNNTTGG 2 cut(s) 1933, 1965
Alw21I GWGCWC 3 cut(s) 1415, 2509, 2652
Alw44I GTGCAC 1 cut(s) 2505
AlwI GGATC 1 cut(s) 388
Ama87I CYCGRG 4 cut(s) 473, 602, 1088, 1651
ApaLI GTGCAC 1 cut(s) 2505
ApoI RAATTY 3 cut(s) 756, 943, 1732
AsiGI ACCGGT 1 cut(s) 2475
AspLEI GCGC 1 cut(s) 2524
AspS9I GGNCC 4 cut(s) 568, 1457, 2071, 2285
AsuC2I CCSGG 7 cut(s) 109, 122, 388, 398, 679, 751, 1225
AvaI CYCGRG 4 cut(s) 473, 602, 1088, 1651
AvaII GGWCC 2 cut(s) 2071, 2285
BaeGI GKGCMC 1 cut(s) 2509
BalI TGGCCA 1 cut(s) 2292
BanI GGYRCC 2 cut(s) 2178, 2521
BanII GRGCYC 2 cut(s) 2008, 2674
BarI GAAGNNNNNNTAC 2 cut(s) 1033, 1065
BbrPI CACGTG 1 cut(s) 1873
BbsI GAAGAC 3 cut(s) 1124, 1143, 1872
Bbv12I GWGCWC 3 cut(s) 1415, 2509, 2652
BccI CCATC 8 cut(s) 221, 553, 1186, 1402, 2075, 2276, 2332, 2794
BceAI ACGGC 1 cut(s) 491
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 4 cut(s) 692, 906, 1914, 2289
BciVI GTATCC 1 cut(s) 1591
BclI TGATCA 2 cut(s) 2124, 2751
BcnI CCSGG 7 cut(s) 109, 122, 388, 398, 679, 751, 1225
BfaI CTAG 4 cut(s) 116, 1007, 1013, 2675
BfmI CTRYAG 3 cut(s) 272, 787, 1467
BfoI RGCGCY 1 cut(s) 2525
BfuI GTATCC 1 cut(s) 1591
BglI GCCNNNNNGGC 1 cut(s) 378
BmcAI AGTACT 1 cut(s) 1945
Bme18I GGWCC 2 cut(s) 2071, 2285
BmeRI GACNNNNNGTC 1 cut(s) 1773
BmeT110I CYCGRG 4 cut(s) 473, 602, 1088, 1651
BmgBI CACGTC 1 cut(s) 1079
BmgT120I GGNCC 4 cut(s) 568, 1457, 2071, 2285
BmiI GGNNCC 4 cut(s) 569, 2005, 2180, 2523
BmrI ACTGGG 2 cut(s) 324, 975
BmsI GCATC 4 cut(s) 1975, 2371, 2490, 2783
BmuI ACTGGG 2 cut(s) 324, 975
BoxI GACNNNNGTC 1 cut(s) 2445
BpiI GAAGAC 3 cut(s) 1124, 1143, 1872
BplI GAGNNNNNCTC 2 cut(s) 2656, 2688
BpmI CTGGAG 1 cut(s) 2022
Bpu10I CCTNAGC 1 cut(s) 1358
BpuEI CTTGAG 5 cut(s) 953, 1312, 1334, 2101, 2506
BpuMI CCSGG 7 cut(s) 109, 122, 388, 398, 679, 751, 1225
BsaAI YACGTR 1 cut(s) 1873
BsaBI GATNNNNATC 1 cut(s) 615
BsaHI GRCGYC 3 cut(s) 1129, 1271, 2522
BsaI GGTCTC 2 cut(s) 1099, 2306
BsaJI CCNNGG 5 cut(s) 256, 690, 750, 859, 1237
BsaWI WCCGGW 2 cut(s) 2475, 2606
BsaXI ACNNNNNCTCC 2 cut(s) 1326, 1356
Bsc4I CCNNNNNNNGG 7 cut(s) 228, 334, 1616, 1913, 2435, 2613, 2849
Bse118I RCCGGY 1 cut(s) 2475
Bse1I ACTGG 6 cut(s) 319, 970, 1459, 1750, 2216, 2747
Bse3DI GCAATG 3 cut(s) 1473, 1806, 1830
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 4 cut(s) 692, 906, 1914, 2289
BseDI CCNNGG 5 cut(s) 256, 690, 750, 859, 1237
BseGI GGATG 6 cut(s) 160, 406, 700, 870, 2343, 2832
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 7 cut(s) 228, 334, 1616, 1913, 2435, 2613, 2849
BseMI GCAATG 3 cut(s) 1473, 1806, 1830
BseMII CTCAG 9 cut(s) 442, 473, 474, 753, 969, 1412, 2089, 2517, 2547
BseNI ACTGG 6 cut(s) 319, 970, 1459, 1750, 2216, 2747
BseRI GAGGAG 4 cut(s) 1350, 1556, 1890, 2750
BseSI GKGCMC 1 cut(s) 2509
BsgI GTGCAG 1 cut(s) 1349
BshNI GGYRCC 2 cut(s) 2178, 2521
BshTI ACCGGT 1 cut(s) 2475
BsiHKAI GWGCWC 3 cut(s) 1415, 2509, 2652
BsiHKCI CYCGRG 4 cut(s) 473, 602, 1088, 1651
BslFI GGGAC 6 cut(s) 361, 676, 1089, 1207, 1648, 2116
BslI CCNNNNNNNGG 7 cut(s) 228, 334, 1616, 1913, 2435, 2613, 2849
BsmBI CGTCTC 1 cut(s) 22
BsmFI GGGAC 6 cut(s) 361, 676, 1089, 1207, 1648, 2116
BsmI GAATGC 3 cut(s) 900, 1317, 1826
Bso31I GGTCTC 2 cut(s) 1099, 2306
BsoBI CYCGRG 4 cut(s) 473, 602, 1088, 1651
Bsp1286I GDGCHC 5 cut(s) 1415, 2008, 2509, 2652, 2674
Bsp143I GATC 5 cut(s) 93, 331, 393, 2124, 2751
BspACI CCGC 4 cut(s) 136, 1876, 2298, 2614
BspCNI CTCAG 9 cut(s) 441, 473, 474, 754, 970, 1411, 2088, 2518, 2548
BspLI GGNNCC 4 cut(s) 569, 2005, 2180, 2523
BspMAI CTGCAG 2 cut(s) 276, 1471
BspPI GGATC 1 cut(s) 388
BspT107I GGYRCC 2 cut(s) 2178, 2521
BspTNI GGTCTC 2 cut(s) 1099, 2306
BsrBI CCGCTC 1 cut(s) 138
BsrDI GCAATG 3 cut(s) 1473, 1806, 1830
BsrFI RCCGGY 1 cut(s) 2475
BsrI ACTGG 6 cut(s) 319, 970, 1459, 1750, 2216, 2747
BssAI RCCGGY 1 cut(s) 2475
BssECI CCNNGG 5 cut(s) 256, 690, 750, 859, 1237
BssMI GATC 5 cut(s) 93, 331, 393, 2124, 2751
BssNI GRCGYC 3 cut(s) 1129, 1271, 2522
BssT1I CCWWGG 2 cut(s) 859, 1237
Bst2UI CCWGG 4 cut(s) 692, 906, 1914, 2289
Bst4CI ACNGT 7 cut(s) 161, 257, 1858, 2030, 2071, 2113, 2545
Bst6I CTCTTC 2 cut(s) 144, 1908
BstACI GRCGYC 3 cut(s) 1129, 1271, 2522
BstAPI GCANNNNNTGC 2 cut(s) 1475, 1832
BstBAI YACGTR 1 cut(s) 1873
BstC8I GCNNGC 5 cut(s) 219, 974, 1205, 1535, 1964
BstDSI CCRYGG 1 cut(s) 256
BstEII GGTNACC 2 cut(s) 36, 2602
BstENI CCTNNNNNAGG 1 cut(s) 2847
BstF5I GGATG 6 cut(s) 160, 406, 700, 870, 2343, 2832
BstH2I RGCGCY 1 cut(s) 2525
BstHHI GCGC 1 cut(s) 2524
BstKTI GATC 5 cut(s) 96, 334, 396, 2127, 2754
BstMBI GATC 5 cut(s) 93, 331, 393, 2124, 2751
BstNI CCWGG 4 cut(s) 692, 906, 1914, 2289
BstNSI RCATGY 1 cut(s) 251
BstPAI GACNNNNGTC 1 cut(s) 2445
BstPI GGTNACC 2 cut(s) 36, 2602
BstSFI CTRYAG 3 cut(s) 272, 787, 1467
BstSLI GKGCMC 1 cut(s) 2509
BstV2I GAAGAC 3 cut(s) 1124, 1143, 1872
BstXI CCANNNNNNTGG 2 cut(s) 698, 1526
BsuI GTATCC 1 cut(s) 1591
BtgI CCRYGG 1 cut(s) 256
BtgZI GCGATG 1 cut(s) 1410
BtrI CACGTC 1 cut(s) 1079
BtsCI GGATG 6 cut(s) 160, 406, 700, 870, 2343, 2832
BtsIMutI CAGTG 3 cut(s) 312, 1743, 2754
Cac8I GCNNGC 5 cut(s) 219, 974, 1205, 1535, 1964
CfoI GCGC 1 cut(s) 2524
Cfr10I RCCGGY 1 cut(s) 2475
Cfr13I GGNCC 4 cut(s) 568, 1457, 2071, 2285
CsiI ACCWGGT 1 cut(s) 1912
Csp6I GTAC 5 cut(s) 50, 1093, 1242, 1944, 2578
CspAI ACCGGT 1 cut(s) 2475
CspCI CAANNNNNGTGG 2 cut(s) 1729, 1764
CviAII CATG 9 cut(s) 248, 1053, 1124, 1692, 1772, 2253, 2279, 2659, 2855
CviQI GTAC 5 cut(s) 50, 1093, 1242, 1944, 2578
DinI GGCGCC 1 cut(s) 2523
DpnI GATC 5 cut(s) 95, 333, 395, 2126, 2753
DpnII GATC 5 cut(s) 93, 331, 393, 2124, 2751
DraI TTTAAA 2 cut(s) 804, 1515
DriI GACNNNNNGTC 1 cut(s) 1773
EaeI YGGCCR 2 cut(s) 504, 2290
Eam1104I CTCTTC 2 cut(s) 144, 1908
Eam1105I GACNNNNNGTC 1 cut(s) 1773
EarI CTCTTC 2 cut(s) 144, 1908
Eco130I CCWWGG 2 cut(s) 859, 1237
Eco24I GRGCYC 2 cut(s) 2008, 2674
Eco31I GGTCTC 2 cut(s) 1099, 2306
Eco47I GGWCC 2 cut(s) 2071, 2285
Eco57I CTGAAG 3 cut(s) 1118, 1580, 2829
Eco72I CACGTG 1 cut(s) 1873
Eco88I CYCGRG 4 cut(s) 473, 602, 1088, 1651
Eco91I GGTNACC 2 cut(s) 36, 2602
EcoNI CCTNNNNNAGG 1 cut(s) 2847
EcoO109I RGGNCCY 1 cut(s) 568
EcoO65I GGTNACC 2 cut(s) 36, 2602
EcoRI GAATTC 1 cut(s) 756
EcoRII CCWGG 4 cut(s) 690, 904, 1912, 2287
EcoT14I CCWWGG 2 cut(s) 859, 1237
EcoT22I ATGCAT 1 cut(s) 1162
EcoT38I GRGCYC 2 cut(s) 2008, 2674
EgeI GGCGCC 1 cut(s) 2523
EheI GGCGCC 1 cut(s) 2523
ErhI CCWWGG 2 cut(s) 859, 1237
Esp3I CGTCTC 1 cut(s) 22
FaeI CATG 9 cut(s) 251, 1056, 1127, 1695, 1775, 2256, 2282, 2662, 2858
FalI AAGNNNNNCTT 4 cut(s) 1306, 1338, 2685, 2717
FaqI GGGAC 6 cut(s) 361, 676, 1089, 1207, 1648, 2116
FatI CATG 9 cut(s) 247, 1052, 1123, 1691, 1771, 2252, 2278, 2658, 2854
FauNDI CATATG 1 cut(s) 1279
FbaI TGATCA 2 cut(s) 2124, 2751
FblI GTMKAC 1 cut(s) 1503
FokI GGATG 6 cut(s) 167, 413, 707, 877, 2350, 2819
FriOI GRGCYC 2 cut(s) 2008, 2674
FspBI CTAG 4 cut(s) 116, 1007, 1013, 2675
GlaI GCGC 1 cut(s) 2523
GsuI CTGGAG 1 cut(s) 2022
HaeII RGCGCY 1 cut(s) 2525
HhaI GCGC 1 cut(s) 2524
Hin1I GRCGYC 3 cut(s) 1129, 1271, 2522
Hin1II CATG 9 cut(s) 251, 1056, 1127, 1695, 1775, 2256, 2282, 2662, 2858
Hin6I GCGC 1 cut(s) 2522
HinP1I GCGC 1 cut(s) 2522
HincII GTYRAC 6 cut(s) 655, 987, 1504, 1548, 2109, 2818
HindII GTYRAC 6 cut(s) 655, 987, 1504, 1548, 2109, 2818
HindIII AAGCTT 3 cut(s) 1064, 1964, 2756
HpaI GTTAAC 1 cut(s) 655
Hpy99I CGWCG 3 cut(s) 80, 83, 1505
HpyCH4III ACNGT 7 cut(s) 161, 257, 1858, 2030, 2071, 2113, 2545
HpyCH4IV ACGT 6 cut(s) 81, 1078, 1129, 1271, 1500, 1872
HpySE526I ACGT 6 cut(s) 81, 1078, 1129, 1271, 1500, 1872
Hsp92I GRCGYC 3 cut(s) 1129, 1271, 2522
Hsp92II CATG 9 cut(s) 251, 1056, 1127, 1695, 1775, 2256, 2282, 2662, 2858
HspAI GCGC 1 cut(s) 2522
KasI GGCGCC 1 cut(s) 2521
Ksp22I TGATCA 2 cut(s) 2124, 2751
KspAI GTTAAC 1 cut(s) 655
Kzo9I GATC 5 cut(s) 93, 331, 393, 2124, 2751
LweI GCATC 4 cut(s) 1975, 2371, 2490, 2783
MabI ACCWGGT 1 cut(s) 1912
MaeI CTAG 4 cut(s) 116, 1007, 1013, 2675
MaeII ACGT 6 cut(s) 81, 1078, 1129, 1271, 1500, 1872
MaeIII GTNAC 8 cut(s) 3, 36, 1605, 1775, 1852, 2191, 2602, 2654
MalI GATC 5 cut(s) 95, 333, 395, 2126, 2753
MbiI CCGCTC 1 cut(s) 138
MboI GATC 5 cut(s) 93, 331, 393, 2124, 2751
MfeI CAATTG 3 cut(s) 169, 1300, 1836
MhlI GDGCHC 5 cut(s) 1415, 2008, 2509, 2652, 2674
MlsI TGGCCA 1 cut(s) 2292
MluNI TGGCCA 1 cut(s) 2292
Mly113I GGCGCC 1 cut(s) 2522
MlyI GAGTC 7 cut(s) 216, 282, 485, 773, 989, 1762, 2209
MmeI TCCRAC 1 cut(s) 679
Mox20I TGGCCA 1 cut(s) 2292
Mph1103I ATGCAT 1 cut(s) 1162
MscI TGGCCA 1 cut(s) 2292
MseI TTAA 8 cut(s) 59, 266, 654, 771, 803, 1514, 1683, 2157
MslI CAYNNNNRTG 1 cut(s) 1782
Msp20I TGGCCA 1 cut(s) 2292
MspA1I CMGCKG 1 cut(s) 2230
MunI CAATTG 3 cut(s) 169, 1300, 1836
Mva1269I GAATGC 3 cut(s) 900, 1317, 1826
MvaI CCWGG 4 cut(s) 692, 906, 1914, 2289
NarI GGCGCC 1 cut(s) 2522
NciI CCSGG 7 cut(s) 109, 122, 388, 398, 679, 751, 1225
NdeI CATATG 1 cut(s) 1279
NdeII GATC 5 cut(s) 93, 331, 393, 2124, 2751
NlaIII CATG 9 cut(s) 251, 1056, 1127, 1695, 1775, 2256, 2282, 2662, 2858
NlaIV GGNNCC 4 cut(s) 569, 2005, 2180, 2523
NmuCI GTSAC 6 cut(s) 3, 36, 1775, 2191, 2602, 2654
NsiI ATGCAT 1 cut(s) 1162
NspI RCATGY 1 cut(s) 251
PaeR7I CTCGAG 2 cut(s) 602, 1088
PcsI WCGNNNNNNNCGW 1 cut(s) 1658
PctI GAATGC 3 cut(s) 900, 1317, 1826
PfeI GAWTC 6 cut(s) 523, 1228, 1402, 1949, 2272, 2377
PflMI CCANNNNNTGG 1 cut(s) 1913
PfoI TCCNGGA 2 cut(s) 396, 1223
PinAI ACCGGT 1 cut(s) 2475
PleI GAGTC 7 cut(s) 215, 282, 484, 772, 988, 1762, 2209
PluTI GGCGCC 1 cut(s) 2525
PmaCI CACGTG 1 cut(s) 1873
PmlI CACGTG 1 cut(s) 1873
PpsI GAGTC 7 cut(s) 215, 282, 484, 772, 988, 1762, 2209
Ppu21I YACGTR 1 cut(s) 1873
PshAI GACNNNNGTC 1 cut(s) 2445
Psp6I CCWGG 4 cut(s) 690, 904, 1912, 2287
PspCI CACGTG 1 cut(s) 1873
PspEI GGTNACC 2 cut(s) 36, 2602
PspGI CCWGG 4 cut(s) 690, 904, 1912, 2287
PspN4I GGNNCC 4 cut(s) 569, 2005, 2180, 2523
PspPI GGNCC 4 cut(s) 568, 1457, 2071, 2285
PspXI VCTCGAGB 2 cut(s) 602, 1088
PstI CTGCAG 2 cut(s) 276, 1471
PvuII CAGCTG 1 cut(s) 2230
RsaI GTAC 5 cut(s) 51, 1094, 1243, 1945, 2579
RsaNI GTAC 5 cut(s) 50, 1093, 1242, 1944, 2578
RseI CAYNNNNRTG 1 cut(s) 1782
SalI GTCGAC 1 cut(s) 1502
SaqAI TTAA 8 cut(s) 59, 266, 654, 771, 803, 1514, 1683, 2157
Sau3AI GATC 5 cut(s) 93, 331, 393, 2124, 2751
Sau96I GGNCC 4 cut(s) 568, 1457, 2071, 2285
ScaI AGTACT 1 cut(s) 1945
SchI GAGTC 7 cut(s) 216, 282, 485, 773, 989, 1762, 2209
SduI GDGCHC 5 cut(s) 1415, 2008, 2509, 2652, 2674
SexAI ACCWGGT 1 cut(s) 1912
SfaNI GCATC 4 cut(s) 1975, 2371, 2490, 2783
SfcI CTRYAG 3 cut(s) 272, 787, 1467
SfoI GGCGCC 1 cut(s) 2523
Sfr274I CTCGAG 2 cut(s) 602, 1088
SinI GGWCC 2 cut(s) 2071, 2285
SlaI CTCGAG 2 cut(s) 602, 1088
SmiMI CAYNNNNRTG 1 cut(s) 1782
SmlI CTYRAG 7 cut(s) 602, 932, 1088, 1291, 1349, 2116, 2485
SmoI CTYRAG 7 cut(s) 602, 932, 1088, 1291, 1349, 2116, 2485
SsiI CCGC 4 cut(s) 136, 1876, 2298, 2614
SspDI GGCGCC 1 cut(s) 2521
SspI AATATT 1 cut(s) 2404
SspMI CTAG 4 cut(s) 116, 1007, 1013, 2675
StyI CCWWGG 2 cut(s) 859, 1237
TaaI ACNGT 7 cut(s) 161, 257, 1858, 2030, 2071, 2113, 2545
TaiI ACGT 6 cut(s) 84, 1081, 1132, 1274, 1503, 1875
TaqI TCGA 9 cut(s) 225, 392, 521, 603, 767, 1089, 1503, 2145, 2365
TatI WGTACW 2 cut(s) 1092, 1943
TauI GCSGC 1 cut(s) 2617
TfiI GAWTC 6 cut(s) 523, 1228, 1402, 1949, 2272, 2377
Tru1I TTAA 8 cut(s) 59, 266, 654, 771, 803, 1514, 1683, 2157
Tru9I TTAA 8 cut(s) 59, 266, 654, 771, 803, 1514, 1683, 2157
TscAI CASTG 3 cut(s) 319, 1750, 2754
TseFI GTSAC 6 cut(s) 3, 36, 1775, 2191, 2602, 2654
Tsp45I GTSAC 6 cut(s) 3, 36, 1775, 2191, 2602, 2654
TspDTI ATGAA 9 cut(s) 268, 605, 900, 968, 1041, 1680, 1819, 2256, 2384
TspGWI ACGGA 1 cut(s) 1650
TspRI CASTG 3 cut(s) 319, 1750, 2754
Van91I CCANNNNNTGG 1 cut(s) 1913
VneI GTGCAC 1 cut(s) 2505
VpaK11BI GGWCC 2 cut(s) 2071, 2285
XagI CCTNNNNNAGG 1 cut(s) 2847
XapI RAATTY 3 cut(s) 756, 943, 1732
XbaI TCTAGA 2 cut(s) 1012, 2674
XceI RCATGY 1 cut(s) 251
XcmI CCANNNNNNNNNTGG 2 cut(s) 552, 2507
XhoI CTCGAG 2 cut(s) 602, 1088
XmiI GTMKAC 1 cut(s) 1503
XspI CTAG 4 cut(s) 116, 1007, 1013, 2675
ZraI GACGTC 2 cut(s) 1130, 1272
ZrmI AGTACT 1 cut(s) 1945
Zsp2I ATGCAT 1 cut(s) 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.