Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0017122

Physical Location & Seq

Genomic Coordinates Forward (+)

26716113 .. 26719246

Gene Structure 3134 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1176400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2676 bp

ATGATTATGAACACTGCTGGGTTTATAGCTGTGTTCATCGTTGTATTTCTTTCAATCCATCTTGTTTTTGCTGCTAATTATGTCCCATCTGATAAAATCTTCTTAAATTGCGGCGGTCCTGCCGAAACGACGGATTCCAATGGCGTGAAATGGACATCGGATGTGGGATCTAAGTTCGCATCCGGTGGAAATTCCTCCACCACTTCCCCCGCCGCCACTCAGGATCCTGCAGTCCCTGAAGTTCCCTTCATGACTGCGAGGGTCTTCCGCTCCCAATTCACCTACAAATTCCCAGTTGCATCCGGTCGAAAATTCATCCGTCTTTACTTTTACCCTGCTTCCTATGCCGAGCTTAATGCCTCCAATGCTGTTTTTACTGTCACTGCTCAGTCCTATACTATTCTGAAGAACTTCAGTGTTGCTCAGACAACTGAGGCTCTGGATTATGTTTTTATTACTAAGGAGTTTATTGTCAATGTCGAGGGTGAGACGTTGGCCGTGTCGTTTACACCCTCTTCCGGGGTTGCGAATGCATTTGCATTTGTGAATGGGATTGAGATTGTGTCAATGCCTGATATTTACAGTGCCACTGATGGTACTACCATGATTGTGGGTCAATCGACTCCTTTCTACATTGATAACAGCACTGCTCTTGAGAATGTTTATCGGATAAATGTGGGTGGGAATGATATCTCGCCGCCAAAAGATACGGGTCTGTTTAGGTCCTGGTACGATGACACTCAGTATCTCTTAGGGGCAGCATTTGGGGCTCCCGAAACTGCTGATCCAAACATGACGATTAGTTACCCTTCGGGCATGCCTACATATATTGCACCAGAGGATGTCTATTCCACTGCCAGATCAATGGGGCCAGATCAAAGAATCAACCTCAATTACAATTTGACTTGGATTTTCTCAATCGACTCTGGATTCTCGTACCTGGTTAGACTACATTTCTGTGAGGTTGCCCAAAATATAACCAAGATTAATCAACGAGTGTTTGATATCTTTCTCAACAAGCAAACTGCTATGGCTGGAGCTGATGTTATTGCCTGGGCGGGAGGCAATGGGATTCCAGTGTACAAGGATTATGTGGTGTTTGTTCCGAATGGGAAACCACAGGTGGATCTATGGCTTGAACTGCATCCAAACACATCTGATAAGCCAAATTACGATGATTCAATCCTAAACGGAGTTGAGATATTCAAGATTAATGATACAACCGGTAACCTTGGGGGGCCTAATCCTCTTCCTCTTCCTAAGCAGGATACCATTGATCCAACCAAGGCTAGACCATCATCAGGTCATGGCAAGCGAAAGAGTGGTCAAACAGCAATTATCGCTGGGGGAGTTGGTGGTGGAATTGCCTTAGCCCTCGTCCTTGGTTTCTTGGCTATTTGTGTGTCACGCCGCCGTAGACAAGGGAAGGACGCAAGTTCAAGTGATGGTCCATCTGGCTGGCTTCCTCTTTCTTTATATGGAAATTCACACTCAGCAGGTTCTGCAAAGACAAACACAACAGGAAGTTACGCTTCCTCCCTTCCTTCAAACCTTTGTCGACATTTCTCGTTTGCTGAGATCAAATCTGCCACCAACAACTTTGATGAGGCTCTACTCCTTGGGGTTGGAGGTTTTGGAAAGGTTTACAAGGGTGAAATTGATGGTGGAACAACCAAAGTAGCAATCAAGCGTGGAAGCGCACTCTCTGACCAAGGTGTGCATGAATTCCAAACTGAGATTGAAATGCTTTCGAAACTTCGCCATCGTCATCTTGTATCTCTGATTGGTTATTGTGAAGAGAACTGTGAAATGATCCTTGTTTATGATTACATGGCTTATGGAACCCTGCGTGAGCATCTGTACAAGACTCAAAAGCCCCCTCTACCCTGGAAGCAAAGGCTTGAGATTTGTATTGGTGCTGCTCGCGGTTTGCACTATCTTCACACTGGTGCCAAGCACACAATCATTCACCGAGATGTGAAGACAACCAATATTCTTTTAGACGAGAAGTGGGTTGCAAAGGTTTCAGATTTTGGGCTGTCAAAAACAGGTCCTACATTGGACAACACGCACGTCAGCACTGTTGTGAAAGGTAGCTTTGGATATTTGGATCCAGAGTACTTCAGGCGGCAACAACTGACCGAAAAATCTGATGTCTACTCTTTTGGGGTTGTTCTCTTCGAAATCTTATGTGCTCGCCCAGCCTTGAACCCAACGCTTCCAAAGGAGCAAGTGAGCTTAGCAGAGTGGGCTGCACATTGCCACAAGAAAGGCATTCTTGAGCACATCCTTGACCCTCATCTCAAGGGCAAGATAACACCAGAATGCTTCAAGAAGTTTGCTGAGACAGCAATGAAATGCGTGTCGGACCAAAGCATCGACAGGCCATCAATGGGAGATGTGCTCTGGAACCTCGAGTTCGCCTTGCAGCTACAGGAGAGTGCAGAAGAGAGTGGCGAGGGTATCGGTGGAGGAATGGACATCGAGGAAGAGCCCTTCATGGGCAGTAAAGGGAAGGACCCCAAAGCAATGCCCGGCTATGATGGTAATGTAACAGACTCAAGGAGCACTGGAATGAGCATGAGCATAGGCGGCCGGAGCCTAGCCAGCGACGACTCTGACGGATTGACGCCAAGTGCCGTGTTCTCACAAATCATGAACCCTAAAGGACGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009741 GO:0009787 GO:0009788 GO:0009791 GO:0009856 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010118 GO:0010483 GO:0010646 GO:0010648 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030054 GO:0030308 GO:0030312 GO:0032501 GO:0032502 GO:0033993 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043207 GO:0043412 GO:0043680 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044426 GO:0044462 GO:0044464 GO:0044703 GO:0044706 GO:0045926 GO:0046777 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051703 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0098542 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

892

Amino Acids

96.94

Weight (kDa)

5.93

Isoelectric Point (pI)

40.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin	PF11721	34 - 169	1.8e-08	Malectin domain
Malectin_like	PF12819	35 - 404	2.4e-35	Malectin-like domain
Malectin	PF11721	221 - 368	1.3e-10	Malectin domain
Pkinase	PF00069	539 - 800	3.3e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	540 - 802	5e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0017122)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51550
fragaria_vesca	FvH4_6g09590
malus_domestica	MD04G1176400.v1.1 MD12G1190700.v1.1
prunus_persica	Prupe.6G295900_v2.0.a1
pyrus_communis	pycom04g15560 pycom12g17790
rosa_chinensis	RchiOBHm_Chr3g0460181
rosa_laevigata	RLG00000025037
rosa_multiflora	Rmu_sc0003763.1_g000007
rosa_roxburghii	Rroxscaffold_6G00419760
rosa_rugosa	Rorug03G0040600
rosa_wichuraiana	Rw3G008320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1487
AccB1I	GGYRCC	1 cut(s)	1949
AccBSI	CCGCTC	1 cut(s)	270
AccI	GTMKAC	3 cut(s)	1417, 1558, 2157
AccII	CGCG	1 cut(s)	1926
AclWI	GGATC	9 cut(s)	175, 218, 231, 779, 1134, 1271, 1807, 2105, 2118
AcoI	YGGCCR	2 cut(s)	495, 2593
AcsI	RAATTY	5 cut(s)	190, 287, 311, 1483, 1724
AcuI	CTGAAG	4 cut(s)	258, 397, 425, 2107
AcyI	GRCGYC	1 cut(s)	2630
AfaI	GTAC	6 cut(s)	598, 731, 938, 1082, 1862, 2120
AfiI	CCNNNNNNNGG	5 cut(s)	518, 519, 1301, 2393, 2501
AgeI	ACCGGT	1 cut(s)	1223
AgsI	TTSAA	9 cut(s)	54, 1139, 1182, 1207, 1440, 1548, 1742, 2209, 2332
AjiI	CACGTC	1 cut(s)	2074
AjnI	CCWGG	4 cut(s)	725, 939, 1052, 1886
AleI	CACNNNNGTG	2 cut(s)	1947, 2084
AluBI	AGCT	6 cut(s)	29, 352, 1040, 2097, 2238, 2431
AluI	AGCT	6 cut(s)	29, 352, 1040, 2097, 2238, 2431
Alw21I	GWGCWC	4 cut(s)	2197, 2286, 2406, 2570
Alw26I	GTCTC	2 cut(s)	482, 2339
AlwI	GGATC	9 cut(s)	175, 218, 231, 779, 1134, 1271, 1807, 2105, 2118
AlwNI	CAGNNNCTG	2 cut(s)	236, 1502
Ama87I	CYCGRG	1 cut(s)	2414
AoxI	GGCC	5 cut(s)	495, 869, 1238, 2384, 2593
ApeKI	GCWGC	5 cut(s)	71, 758, 1919, 2252, 2428
ApoI	RAATTY	5 cut(s)	190, 287, 311, 1483, 1724
ArsI	GACNNNNNNTTYG	2 cut(s)	1309, 1341
AseI	ATTAAT	2 cut(s)	987, 1212
AsiGI	ACCGGT	1 cut(s)	1223
Asp700I	GAANNNNTTC	1 cut(s)	410
AspLEI	GCGC	1 cut(s)	1700
AspS9I	GGNCC	8 cut(s)	116, 723, 869, 1238, 1448, 2051, 2368, 2518
AsuC2I	CCSGG	2 cut(s)	520, 2535
AsuHPI	GGTGA	4 cut(s)	271, 497, 1664, 1961
AsuII	TTCGAA	2 cut(s)	1751, 2181
AvaI	CYCGRG	1 cut(s)	2414
AvaII	GGWCC	6 cut(s)	116, 723, 1448, 2051, 2368, 2518
BamHI	GGATCC	2 cut(s)	223, 2110
BanI	GGYRCC	1 cut(s)	1949
BanII	GRGCYC	2 cut(s)	772, 2496
BbsI	GAAGAC	2 cut(s)	256, 1988
Bbv12I	GWGCWC	4 cut(s)	2197, 2286, 2406, 2570
BbvI	GCAGC	5 cut(s)	58, 770, 1906, 2239, 2440
BceAI	ACGGC	3 cut(s)	482, 1398, 2624
BcgI	CGANNNNNNTGC	2 cut(s)	338, 372
BciT130I	CCWGG	4 cut(s)	727, 941, 1054, 1888
BciVI	GTATCC	1 cut(s)	1261
BcnI	CCSGG	2 cut(s)	520, 2535
BcoDI	GTCTC	2 cut(s)	482, 2339
BfaI	CTAG	2 cut(s)	1290, 2603
BfmI	CTRYAG	2 cut(s)	228, 2432
BfuAI	ACCTGC	1 cut(s)	1487
BfuI	GTATCC	1 cut(s)	1261
BlpI	GCTNAGC	1 cut(s)	2239
BmcAI	AGTACT	1 cut(s)	2120
Bme1390I	CCNGG	6 cut(s)	520, 727, 941, 1054, 1888, 2535
Bme18I	GGWCC	6 cut(s)	116, 723, 1448, 2051, 2368, 2518
BmeT110I	CYCGRG	1 cut(s)	2414
BmgBI	CACGTC	1 cut(s)	2074
BmgT120I	GGNCC	8 cut(s)	116, 723, 869, 1238, 1448, 2051, 2368, 2518
BmrFI	CCNGG	6 cut(s)	520, 727, 941, 1054, 1888, 2535
BmrI	ACTGGG	1 cut(s)	287
BmsI	GCATC	5 cut(s)	188, 308, 1153, 1864, 2385
BmuI	ACTGGG	1 cut(s)	287
BpiI	GAAGAC	2 cut(s)	256, 1988
BplI	GAGNNNNNCTC	2 cut(s)	2388, 2420
BpmI	CTGGAG	1 cut(s)	1056
Bpu10I	CCTNAGC	2 cut(s)	1260, 1369
Bpu1102I	GCTNAGC	1 cut(s)	2239
Bpu14I	TTCGAA	2 cut(s)	1751, 2181
BpuEI	CTTGAG	5 cut(s)	674, 1922, 2288, 2300, 2545
BpuMI	CCSGG	2 cut(s)	520, 2535
BsaHI	GRCGYC	1 cut(s)	2630
BsaJI	CCNNGG	8 cut(s)	519, 1053, 1231, 1284, 1381, 1618, 1711, 1886
BsaWI	WCCGGW	3 cut(s)	182, 302, 1223
BsaXI	ACNNNNNCTCC	4 cut(s)	1029, 1059, 1520, 1550
Bsc4I	CCNNNNNNNGG	5 cut(s)	518, 519, 1301, 2393, 2501
Bse118I	RCCGGY	1 cut(s)	1223
Bse1I	ACTGG	4 cut(s)	293, 1076, 1951, 2575
Bse3DI	GCAATG	4 cut(s)	1072, 2257, 2358, 2535
BseBI	CCWGG	4 cut(s)	727, 941, 1054, 1888
BseDI	CCNNGG	8 cut(s)	519, 1053, 1231, 1284, 1381, 1618, 1711, 1886
BseGI	GGATG	7 cut(s)	166, 179, 299, 315, 847, 1144, 2286
BseLI	CCNNNNNNNGG	5 cut(s)	518, 519, 1301, 2393, 2501
BseMI	GCAATG	4 cut(s)	1072, 2257, 2358, 2535
BseMII	CTCAG	9 cut(s)	233, 401, 423, 437, 755, 1506, 1566, 1725, 2334
BseNI	ACTGG	4 cut(s)	293, 1076, 1951, 2575
BseX3I	CGGCCG	1 cut(s)	2593
BseXI	GCAGC	5 cut(s)	58, 770, 1906, 2239, 2440
BseYI	CCCAGC	3 cut(s)	17, 1343, 2200
BsgI	GTGCAG	2 cut(s)	2238, 2463
Bsh1236I	CGCG	1 cut(s)	1926
Bsh1285I	CGRYCG	2 cut(s)	307, 2596
BshFI	GGCC	5 cut(s)	497, 871, 1240, 2386, 2595
BshNI	GGYRCC	1 cut(s)	1949
BshTI	ACCGGT	1 cut(s)	1223
BsiEI	CGRYCG	2 cut(s)	307, 2596
BsiHKAI	GWGCWC	4 cut(s)	2197, 2286, 2406, 2570
BsiHKCI	CYCGRG	1 cut(s)	2414
BsiSI	CCGG	6 cut(s)	183, 303, 519, 1224, 2535, 2596
BslFI	GGGAC	2 cut(s)	68, 218
BslI	CCNNNNNNNGG	5 cut(s)	518, 519, 1301, 2393, 2501
BsmAI	GTCTC	2 cut(s)	482, 2339
BsmBI	CGTCTC	1 cut(s)	482
BsmFI	GGGAC	2 cut(s)	68, 218
BsmI	GAATGC	3 cut(s)	535, 2274, 2330
BsnI	GGCC	5 cut(s)	497, 871, 1240, 2386, 2595
BsoBI	CYCGRG	1 cut(s)	2414
Bsp119I	TTCGAA	2 cut(s)	1751, 2181
Bsp1286I	GDGCHC	6 cut(s)	772, 2197, 2286, 2406, 2496, 2570
Bsp1407I	TGTACA	2 cut(s)	1080, 1860
Bsp1720I	GCTNAGC	1 cut(s)	2239
BspANI	GGCC	5 cut(s)	497, 871, 1240, 2386, 2595
BspCNI	CTCAG	9 cut(s)	232, 400, 424, 436, 754, 1505, 1567, 1726, 2335
BspFNI	CGCG	1 cut(s)	1926
BspHI	TCATGA	2 cut(s)	249, 2655
BspMAI	CTGCAG	1 cut(s)	232
BspMI	ACCTGC	1 cut(s)	1487
BspPI	GGATC	9 cut(s)	175, 218, 231, 779, 1134, 1271, 1807, 2105, 2118
BspQI	GCTCTTC	1 cut(s)	2484
BspT104I	TTCGAA	2 cut(s)	1751, 2181
BspT107I	GGYRCC	1 cut(s)	1949
BsrBI	CCGCTC	1 cut(s)	270
BsrDI	GCAATG	4 cut(s)	1072, 2257, 2358, 2535
BsrFI	RCCGGY	1 cut(s)	1223
BsrGI	TGTACA	2 cut(s)	1080, 1860
BsrI	ACTGG	4 cut(s)	293, 1076, 1951, 2575
BssAI	RCCGGY	1 cut(s)	1223
BssECI	CCNNGG	8 cut(s)	519, 1053, 1231, 1284, 1381, 1618, 1711, 1886
BssNI	GRCGYC	1 cut(s)	2630
BssT1I	CCWWGG	5 cut(s)	1231, 1284, 1381, 1618, 1711
Bst2UI	CCWGG	4 cut(s)	727, 941, 1054, 1888
Bst4CI	ACNGT	4 cut(s)	379, 584, 1805, 2083
Bst6I	CTCTTC	7 cut(s)	520, 1254, 1260, 1791, 2183, 2442, 2484
BstACI	GRCGYC	1 cut(s)	2630
BstAPI	GCANNNNNTGC	1 cut(s)	1502
BstAUI	TGTACA	2 cut(s)	1080, 1860
BstBI	TTCGAA	2 cut(s)	1751, 2181
BstC8I	GCNNGC	6 cut(s)	818, 1313, 1460, 1924, 2197, 2608
BstEII	GGTNACC	1 cut(s)	1226
BstF5I	GGATG	7 cut(s)	166, 179, 299, 315, 847, 1144, 2286
BstFNI	CGCG	1 cut(s)	1926
BstHHI	GCGC	1 cut(s)	1700
BstMAI	GTCTC	2 cut(s)	482, 2339
BstMCI	CGRYCG	2 cut(s)	307, 2596
BstNI	CCWGG	4 cut(s)	727, 941, 1054, 1888
BstNSI	RCATGY	1 cut(s)	820
BstPI	GGTNACC	1 cut(s)	1226
BstSCI	CCNGG	6 cut(s)	518, 725, 939, 1052, 1886, 2533
BstSFI	CTRYAG	2 cut(s)	228, 2432
BstUI	CGCG	1 cut(s)	1926
BstV1I	GCAGC	5 cut(s)	58, 770, 1906, 2239, 2440
BstV2I	GAAGAC	2 cut(s)	256, 1988
BstX2I	RGATCY	4 cut(s)	167, 223, 1126, 2110
BstXI	CCANNNNNNTGG	3 cut(s)	610, 865, 1458
BstYI	RGATCY	4 cut(s)	167, 223, 1126, 2110
BstZI	CGGCCG	1 cut(s)	2593
BsuI	GTATCC	1 cut(s)	1261
BsuRI	GGCC	5 cut(s)	497, 871, 1240, 2386, 2595
BtrI	CACGTC	1 cut(s)	2074
BtsCI	GGATG	7 cut(s)	166, 179, 299, 315, 847, 1144, 2286
BtsI	GCAGTG	4 cut(s)	12, 381, 645, 852
BveI	ACCTGC	1 cut(s)	1487
Cac8I	GCNNGC	6 cut(s)	818, 1313, 1460, 1924, 2197, 2608
CaiI	CAGNNNCTG	2 cut(s)	236, 1502
CciI	TCATGA	2 cut(s)	249, 2655
CfoI	GCGC	1 cut(s)	1700
Cfr10I	RCCGGY	1 cut(s)	1223
Cfr13I	GGNCC	8 cut(s)	116, 723, 869, 1238, 1448, 2051, 2368, 2518
CseI	GACGC	2 cut(s)	1439, 2638
CsiI	ACCWGGT	1 cut(s)	939
Csp6I	GTAC	6 cut(s)	597, 730, 937, 1081, 1861, 2119
CspAI	ACCGGT	1 cut(s)	1223
CviQI	GTAC	6 cut(s)	597, 730, 937, 1081, 1861, 2119
EaeI	YGGCCR	2 cut(s)	495, 2593
EagI	CGGCCG	1 cut(s)	2593
Eam1104I	CTCTTC	7 cut(s)	520, 1254, 1260, 1791, 2183, 2442, 2484
EarI	CTCTTC	7 cut(s)	520, 1254, 1260, 1791, 2183, 2442, 2484
EclXI	CGGCCG	1 cut(s)	2593
Eco130I	CCWWGG	5 cut(s)	1231, 1284, 1381, 1618, 1711
Eco24I	GRGCYC	2 cut(s)	772, 2496
Eco32I	GATATC	2 cut(s)	691, 1006
Eco47I	GGWCC	6 cut(s)	116, 723, 1448, 2051, 2368, 2518
Eco52I	CGGCCG	1 cut(s)	2593
Eco57I	CTGAAG	4 cut(s)	258, 397, 425, 2107
Eco88I	CYCGRG	1 cut(s)	2414
Eco91I	GGTNACC	1 cut(s)	1226
EcoO109I	RGGNCCY	4 cut(s)	723, 1238, 2051, 2518
EcoO65I	GGTNACC	1 cut(s)	1226
EcoRI	GAATTC	1 cut(s)	1724
EcoRII	CCWGG	4 cut(s)	725, 939, 1052, 1886
EcoRV	GATATC	2 cut(s)	691, 1006
EcoT14I	CCWWGG	5 cut(s)	1231, 1284, 1381, 1618, 1711
EcoT22I	ATGCAT	1 cut(s)	535
EcoT38I	GRGCYC	2 cut(s)	772, 2496
ErhI	CCWWGG	5 cut(s)	1231, 1284, 1381, 1618, 1711
Esp3I	CGTCTC	1 cut(s)	482
FalI	AAGNNNNNCTT	4 cut(s)	1516, 1548, 2262, 2294
FaqI	GGGAC	2 cut(s)	68, 218
FauI	CCCGC	2 cut(s)	217, 1051
FblI	GTMKAC	3 cut(s)	1417, 1558, 2157
FokI	GGATG	7 cut(s)	166, 173, 286, 302, 854, 1131, 2273
FriOI	GRGCYC	2 cut(s)	772, 2496
FspBI	CTAG	2 cut(s)	1290, 2603
GlaI	GCGC	1 cut(s)	1699
GsaI	CCCAGC	3 cut(s)	21, 1347, 2204
GsuI	CTGGAG	1 cut(s)	1056
HaeIII	GGCC	5 cut(s)	497, 871, 1240, 2386, 2595
HapII	CCGG	6 cut(s)	183, 303, 519, 1224, 2535, 2596
HgaI	GACGC	2 cut(s)	1439, 2638
HhaI	GCGC	1 cut(s)	1700
Hin1I	GRCGYC	1 cut(s)	2630
Hin6I	GCGC	1 cut(s)	1698
HinP1I	GCGC	1 cut(s)	1698
HincII	GTYRAC	1 cut(s)	1559
HindII	GTYRAC	1 cut(s)	1559
HpaII	CCGG	6 cut(s)	183, 303, 519, 1224, 2535, 2596
HphI	GGTGA	4 cut(s)	271, 497, 1664, 1961
Hpy166II	GTNNAC	6 cut(s)	507, 1081, 1418, 1559, 1645, 2158
Hpy8I	GTNNAC	6 cut(s)	507, 1081, 1418, 1559, 1645, 2158
Hpy99I	CGWCG	2 cut(s)	133, 2615
HpyAV	CCTTC	7 cut(s)	256, 819, 1420, 1550, 1554, 2506, 2509
HpyCH4III	ACNGT	4 cut(s)	379, 584, 1805, 2083
HpyCH4IV	ACGT	3 cut(s)	491, 2073, 2671
HpySE526I	ACGT	3 cut(s)	491, 2073, 2671
Hsp92I	GRCGYC	1 cut(s)	2630
HspAI	GCGC	1 cut(s)	1698
LguI	GCTCTTC	1 cut(s)	2484
LmnI	GCTCC	6 cut(s)	275, 775, 1037, 2227, 2565, 2598
Lsp1109I	GCAGC	5 cut(s)	58, 770, 1906, 2239, 2440
LweI	GCATC	5 cut(s)	188, 308, 1153, 1864, 2385
MabI	ACCWGGT	1 cut(s)	939
MaeI	CTAG	2 cut(s)	1290, 2603
MaeII	ACGT	3 cut(s)	491, 2073, 2671
MaeIII	GTNAC	6 cut(s)	379, 803, 1226, 1404, 1526, 2551
MbiI	CCGCTC	1 cut(s)	270
MflI	RGATCY	4 cut(s)	167, 223, 1126, 2110
MhlI	GDGCHC	6 cut(s)	772, 2197, 2286, 2406, 2496, 2570
MlyI	GAGTC	5 cut(s)	616, 917, 1861, 2552, 2609
MmeI	TCCRAC	3 cut(s)	1304, 1606, 2346
Mph1103I	ATGCAT	1 cut(s)	535
MroXI	GAANNNNTTC	1 cut(s)	410
MseI	TTAA	4 cut(s)	104, 354, 987, 1212
MslI	CAYNNNNRTG	7 cut(s)	608, 957, 1947, 1974, 2084, 2323, 2573
MspI	CCGG	6 cut(s)	183, 303, 519, 1224, 2535, 2596
MspR9I	CCNGG	6 cut(s)	520, 727, 941, 1054, 1888, 2535
Mva1269I	GAATGC	3 cut(s)	535, 2274, 2330
MvaI	CCWGG	4 cut(s)	727, 941, 1054, 1888
MvnI	CGCG	1 cut(s)	1926
NciI	CCSGG	2 cut(s)	520, 2535
NmeAIII	GCCGAG	1 cut(s)	373
NmuCI	GTSAC	2 cut(s)	379, 1404
NsiI	ATGCAT	1 cut(s)	535
NspI	RCATGY	1 cut(s)	820
NspV	TTCGAA	2 cut(s)	1751, 2181
OliI	CACNNNNGTG	2 cut(s)	1947, 2084
PaeI	GCATGC	1 cut(s)	820
PaeR7I	CTCGAG	1 cut(s)	2414
PagI	TCATGA	2 cut(s)	249, 2655
PciSI	GCTCTTC	1 cut(s)	2484
PctI	GAATGC	3 cut(s)	535, 2274, 2330
PdmI	GAANNNNTTC	1 cut(s)	410
PfeI	GAWTC	5 cut(s)	134, 882, 930, 1072, 1178
PinAI	ACCGGT	1 cut(s)	1223
PleI	GAGTC	5 cut(s)	616, 917, 1861, 2552, 2609
PpsI	GAGTC	5 cut(s)	616, 917, 1861, 2552, 2609
PpuMI	RGGWCCY	3 cut(s)	723, 2051, 2518
PshBI	ATTAAT	2 cut(s)	987, 1212
Psp5II	RGGWCCY	3 cut(s)	723, 2051, 2518
Psp6I	CCWGG	4 cut(s)	725, 939, 1052, 1886
PspEI	GGTNACC	1 cut(s)	1226
PspFI	CCCAGC	3 cut(s)	17, 1343, 2200
PspGI	CCWGG	4 cut(s)	725, 939, 1052, 1886
PspPI	GGNCC	8 cut(s)	116, 723, 869, 1238, 1448, 2051, 2368, 2518
PspPPI	RGGWCCY	3 cut(s)	723, 2051, 2518
PspXI	VCTCGAGB	1 cut(s)	2414
PstI	CTGCAG	1 cut(s)	232
PstNI	CAGNNNCTG	2 cut(s)	236, 1502
PsuI	RGATCY	4 cut(s)	167, 223, 1126, 2110
RsaI	GTAC	6 cut(s)	598, 731, 938, 1082, 1862, 2120
RsaNI	GTAC	6 cut(s)	597, 730, 937, 1081, 1861, 2119
RseI	CAYNNNNRTG	7 cut(s)	608, 957, 1947, 1974, 2084, 2323, 2573
SalI	GTCGAC	1 cut(s)	1557
SapI	GCTCTTC	1 cut(s)	2484
SaqAI	TTAA	4 cut(s)	104, 354, 987, 1212
Sau96I	GGNCC	8 cut(s)	116, 723, 869, 1238, 1448, 2051, 2368, 2518
ScaI	AGTACT	1 cut(s)	2120
SchI	GAGTC	5 cut(s)	616, 917, 1861, 2552, 2609
ScrFI	CCNGG	6 cut(s)	520, 727, 941, 1054, 1888, 2535
SduI	GDGCHC	6 cut(s)	772, 2197, 2286, 2406, 2496, 2570
SexAI	ACCWGGT	1 cut(s)	939
SfaNI	GCATC	5 cut(s)	188, 308, 1153, 1864, 2385
SfcI	CTRYAG	2 cut(s)	228, 2432
Sfr274I	CTCGAG	1 cut(s)	2414
SfuI	TTCGAA	2 cut(s)	1751, 2181
SinI	GGWCC	6 cut(s)	116, 723, 1448, 2051, 2368, 2518
SlaI	CTCGAG	1 cut(s)	2414
SmiMI	CAYNNNNRTG	7 cut(s)	608, 957, 1947, 1974, 2084, 2323, 2573
SmlI	CTYRAG	6 cut(s)	653, 1901, 2279, 2303, 2414, 2560
SmoI	CTYRAG	6 cut(s)	653, 1901, 2279, 2303, 2414, 2560
SphI	GCATGC	1 cut(s)	820
SspI	AATATT	1 cut(s)	1993
SspMI	CTAG	2 cut(s)	1290, 2603
StyD4I	CCNGG	6 cut(s)	518, 725, 939, 1052, 1886, 2533
StyI	CCWWGG	5 cut(s)	1231, 1284, 1381, 1618, 1711
TaaI	ACNGT	4 cut(s)	379, 584, 1805, 2083
TaiI	ACGT	3 cut(s)	494, 2076, 2674
TaqII	GACCGA	1 cut(s)	2156
TatI	WGTACW	3 cut(s)	1080, 1860, 2118
TauI	GCSGC	6 cut(s)	114, 215, 700, 1413, 2131, 2595
TfiI	GAWTC	5 cut(s)	134, 882, 930, 1072, 1178
Tru1I	TTAA	4 cut(s)	104, 354, 987, 1212
Tru9I	TTAA	4 cut(s)	104, 354, 987, 1212
TseFI	GTSAC	2 cut(s)	379, 1404
TseI	GCWGC	5 cut(s)	71, 758, 1919, 2252, 2428
Tsp45I	GTSAC	2 cut(s)	379, 1404
TspDTI	ATGAA	8 cut(s)	23, 25, 238, 304, 1737, 2369, 2488, 2672
TspGWI	ACGGA	4 cut(s)	146, 308, 1206, 2637
VpaK11BI	GGWCC	6 cut(s)	116, 723, 1448, 2051, 2368, 2518
VspI	ATTAAT	2 cut(s)	987, 1212
XapI	RAATTY	5 cut(s)	190, 287, 311, 1483, 1724
XceI	RCATGY	1 cut(s)	820
XhoI	CTCGAG	1 cut(s)	2414
XmiI	GTMKAC	3 cut(s)	1417, 1558, 2157
XmnI	GAANNNNTTC	1 cut(s)	410
XspI	CTAG	2 cut(s)	1290, 2603
ZrmI	AGTACT	1 cut(s)	2120
Zsp2I	ATGCAT	1 cut(s)	535

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.