Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0017122

Physical Location & Seq

Genomic Coordinates Forward (+)

27336584 .. 27339262

Gene Structure 2679 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1190700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2679 bp

ATGATAATGAACACTGCTGGGTTTGTAGCTGTGTTCGTCTTAGTATTTCTTTCAATCCATGTTGTTTTGTCTGCAAATTATGTCCCATCTGATAAAATCTTCCTAAATTGCGGTGGTCCTCCCGAAACCACCGACTCCGATGGCCTGAAATGGACATCGGATGTGGGCTCTAAGTTTGCATCCGGTGGAAATTCCTCCACCACTTCCGCCGCCGCCACTCAGGACCCTTCAGTCCCTGACGTTCCCTTCATGACTGCGAGGGTCTTCCGCTCCGAATTCACCTACAAATTCCCTGTTGCATCCGGTCGAAAGTTCATCCGTCTATACTTTTACCCTGCATCCTATGCCGGCCTCAATGCCTCCAATGCTGTTTTTACTGTCACTGCTCAGTCCTACACTATTCTCAAGAACTTCAGTGTTGCTCAGACAACTGAGGCTCTGGATTATGTTTATATTACCAAGGAGTTTTCTGTCAATGTCGATGGCGAGACGTTGGATGTTTCGTTTGCACCCTCTTCCGGGGTTGCGAATGCGTTTGCATTTGTGAATGGGATTGAGATTGTGTCAATGCCTGATATTTACAGTGCCACTGATGGTACTATCATGATTGTGGGTCAATCGGCTCCTTTCTACATTGATAATACCACTGCACTTGAGAATGTTTATCGGTTAAATGTGGGTGGGAATGATATCTCGCCGCCAGATGATACGGGTCTGTTTAGGTCCTGGTATGATGACACCCAGTACCTCTTAGGGGCAGCGTTTGGGGTTACTGGAACTGCTGATCCAAACATGACGATTAGTTACCCTACGACCATGCCTACTTATGTTGCACCACAAGATGTCTATTCCACTGCCAGATCAATGGGGCCAAATGCACAAATCAACCTCAATTACAATCTGACTTGGATTTTCTCAATCGACTCTGGATTCTCGTACCTGGTTAGGCTACATTTCTGTGAGGTTGCCCAAAATATAACGAAGGTTAATCAACGAGTATTCGATATCTTTCTCAACAATCAAACTGCTCAGTCTGGAGCTGATGTTGTTGCCTGGGCAGGAGGCAATGGAATTCCGGTGTATAAGGATTACGTGGTGTTTGTTCCGAATGGGAGTCCGCAGGTGGATCTATGGCTTGAGCTGTATCCAGACACTAGTGATAAGCCAAATTACTATGATTCAATCCTAAACGGAGTTGAGATATTCAAGATTAATGATACAACCGGTAACCTTGGAGGGCTTAATCCTGTTCCTCTTCCTAAGCAGGATATCATTGATCCAGCCATGGTTAGACTATCATCAGGTCATGGCAAGCCGAAGAGTGGTCAAAGAGCAATTATCTCTGGGGGAGTTAGTGGTGGAATTGCCGTAGCCCTCGTCCTTGGTTTCTTGGCTGTTTGTGTGTCTCGCCGTCGTAGACAACGGAAGGACGCAAGTTCAAGTGATGGGCCATCTGGCTGGCTCCCTCTTTCTTTATATGGAAACTCACACTCTGCAGGTTCTGCAAAGACAAACACAACAGGGAGTTATGCTTCCTCCCTTCCTTCAAATCTTTGTCGACATTTCTCGTTTGCTGAGATCAAATCTGCCACCAACAACTTTGATGAGGCTCTAATCCTCGGGGTGGGAGGTTTTGGAAAGGTTTACAAGGGTGAAGTTGATGGTGGAACAACCAAAGTAGCAATCAAGCGTGGAAGCGCACTGTCTGACCAAGGTGTGCATGAATTCCAAACTGAGATTGAAATGCTCTCAAAGCTTCGCCATCGTCATCTCGTTTCTCTGATTGGCTATTGTGAAGAAAACTGTGAAATGATCCTTGTTTATGATTACATGGCTTACGGAACCCTGCGTGAGCATCTGTACAAGACCCAAAAGCCCCCTCTACCCTGGAAGCAAAGGCTTGAGATTTGTATTGGAGCTGCTCGCGGTTTGCACTATCTTCACACTGGTGCCAAGCACACCATCATCCACCGAGATGTGAAGACAACTAATATTCTCTTAGACGAGAAGTGGGTCGCAAAGGTTTCAGATTTTGGGCTGTCAAAAACAGGTCCTACATTGGACAACACGCACGTCAGCACTGTCGTGAAAGGTAGCTTTGGGTATTTGGATCCAGAGTACTTCAGGCGGCAACAACTGACCGAAAAGTCTGATGTCTACTCATTTGGGGTTGTCCTTTTCGAGATCTTATGTGCTCGCCCGGCCTTGAACCCAACGCTTCCAAAGGAGCAAGTGAGCTTAGCAGAGTGGGCTGCCCATTGCCACAAGAAAGGCATTCTGGAGCACATCCTCGACCCTCATCTCAAGGGGAAGATAACGCCAGAGTGCTTCAAGAAGTTCGCTGAGACAGCAATGAAATGCGTTTCAGACCAAAGCATCAACAGGCCATCAATGGGAGATGTTCTTTGGAACCTTGAGTTCGCTTTGCAGCTACAGGAGAGTGCAGAAGAGAGCGGCAAGGCTATCGGCGGAGGAATGGACATCGAGGAAGAGCCCCTCGTGGACTGTAAAGGGAAGAAGGACCCCGAAGCAATGCCCGGCTATGATGGTAATATAACAGACTCAAGGAGCACCGGAATGAGCATGAGCATAGGCGGCCGGAGCCTAGCTAGCGTCGACTCTGATGGATTGACACCAAGTGCCGTGTTCTCGCAAATCATGCAGCCTAAAGGACGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009741 GO:0009787 GO:0009788 GO:0009791 GO:0009856 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010118 GO:0010483 GO:0010646 GO:0010648 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030054 GO:0030308 GO:0030312 GO:0032501 GO:0032502 GO:0033993 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043207 GO:0043412 GO:0043680 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044426 GO:0044462 GO:0044464 GO:0044703 GO:0044706 GO:0045926 GO:0046777 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051703 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0098542 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

893

Amino Acids

97.03

Weight (kDa)

5.76

Isoelectric Point (pI)

39.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin	PF11721	34 - 169	8.2e-09	Malectin domain
Malectin_like	PF12819	35 - 404	1.8e-34	Malectin-like domain
Malectin	PF11721	223 - 367	2.4e-10	Malectin domain
Pkinase	PF00069	539 - 800	2.3e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	540 - 802	4.7e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0017122)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51550
fragaria_vesca	FvH4_6g09590
malus_domestica	MD04G1176400.v1.1 MD12G1190700.v1.1
prunus_persica	Prupe.6G295900_v2.0.a1
pyrus_communis	pycom04g15560 pycom12g17790
rosa_chinensis	RchiOBHm_Chr3g0460181
rosa_laevigata	RLG00000025037
rosa_multiflora	Rmu_sc0003763.1_g000007
rosa_roxburghii	Rroxscaffold_6G00419760
rosa_rugosa	Rorug03G0040600
rosa_wichuraiana	Rw3G008320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1111
AasI	GACNNNNNNGTC	2 cut(s)	230, 2146
Acc36I	ACCTGC	2 cut(s)	1111, 1487
AccB1I	GGYRCC	1 cut(s)	1949
AccBSI	CCGCTC	2 cut(s)	270, 2454
AccI	GTMKAC	4 cut(s)	1417, 1558, 2157, 2616
AccII	CGCG	1 cut(s)	1926
AclWI	GGATC	6 cut(s)	779, 1134, 1271, 1807, 2105, 2118
AcoI	YGGCCR	1 cut(s)	2596
AcsI	RAATTY	5 cut(s)	190, 275, 287, 1071, 1724
AcuI	CTGAAG	3 cut(s)	213, 397, 2107
AdeI	CACNNNGTG	1 cut(s)	2639
AfaI	GTAC	5 cut(s)	598, 746, 938, 1862, 2120
AfiI	CCNNNNNNNGG	6 cut(s)	518, 519, 754, 1322, 1624, 2393
AgeI	ACCGGT	1 cut(s)	1223
AgsI	TTSAA	8 cut(s)	54, 1182, 1207, 1440, 1548, 1742, 2209, 2332
AhlI	ACTAGT	1 cut(s)	1154
AjiI	CACGTC	1 cut(s)	2074
AjnI	CCWGG	4 cut(s)	725, 939, 1052, 1886
AleI	CACNNNNGTG	2 cut(s)	1947, 2084
AloI	GAACNNNNNNTCC	2 cut(s)	769, 801
AluBI	AGCT	9 cut(s)	29, 1040, 1141, 1756, 1919, 2097, 2238, 2431, 2609
AluI	AGCT	9 cut(s)	29, 1040, 1141, 1756, 1919, 2097, 2238, 2431, 2609
Alw21I	GWGCWC	3 cut(s)	2197, 2286, 2573
Alw26I	GTCTC	3 cut(s)	482, 1410, 2339
AlwI	GGATC	6 cut(s)	779, 1134, 1271, 1807, 2105, 2118
AlwNI	CAGNNNCTG	2 cut(s)	236, 1502
Ama87I	CYCGRG	1 cut(s)	1619
AoxI	GGCC	7 cut(s)	142, 349, 869, 1448, 2202, 2384, 2596
ApeKI	GCWGC	5 cut(s)	758, 1919, 2252, 2428, 2662
ApoI	RAATTY	5 cut(s)	190, 275, 287, 1071, 1724
AseI	ATTAAT	1 cut(s)	1212
AsiGI	ACCGGT	1 cut(s)	1223
AspLEI	GCGC	1 cut(s)	1700
AspS9I	GGNCC	7 cut(s)	116, 223, 723, 869, 1448, 2051, 2521
AsuC2I	CCSGG	3 cut(s)	520, 2201, 2538
AsuHPI	GGTGA	2 cut(s)	271, 1664
AsuNHI	GCTAGC	1 cut(s)	2609
AvaI	CYCGRG	1 cut(s)	1619
AvaII	GGWCC	5 cut(s)	116, 223, 723, 2051, 2521
BamHI	GGATCC	1 cut(s)	2110
BanI	GGYRCC	1 cut(s)	1949
BanII	GRGCYC	2 cut(s)	170, 2496
BauI	CACGAG	1 cut(s)	2498
BbsI	GAAGAC	2 cut(s)	256, 1988
Bbv12I	GWGCWC	3 cut(s)	2197, 2286, 2573
BbvI	GCAGC	5 cut(s)	770, 1906, 2239, 2440, 2674
BceAI	ACGGC	3 cut(s)	1352, 1395, 2627
BcgI	CGANNNNNNTGC	2 cut(s)	2446, 2480
BciT130I	CCWGG	4 cut(s)	727, 941, 1054, 1888
BciVI	GTATCC	1 cut(s)	1155
BcnI	CCSGG	3 cut(s)	520, 2201, 2538
BcoDI	GTCTC	3 cut(s)	482, 1410, 2339
BcuI	ACTAGT	1 cut(s)	1154
BfaI	CTAG	3 cut(s)	1155, 2606, 2610
BfmI	CTRYAG	2 cut(s)	1494, 2432
BfuAI	ACCTGC	2 cut(s)	1111, 1487
BfuI	GTATCC	1 cut(s)	1155
BglII	AGATCT	1 cut(s)	2184
BlpI	GCTNAGC	1 cut(s)	2239
BmcAI	AGTACT	1 cut(s)	2120
Bme1390I	CCNGG	7 cut(s)	520, 727, 941, 1054, 1888, 2201, 2538
Bme18I	GGWCC	5 cut(s)	116, 223, 723, 2051, 2521
BmeT110I	CYCGRG	1 cut(s)	1619
BmgBI	CACGTC	1 cut(s)	2074
BmgT120I	GGNCC	7 cut(s)	116, 223, 723, 869, 1448, 2051, 2521
BmrFI	CCNGG	7 cut(s)	520, 727, 941, 1054, 1888, 2201, 2538
BmrI	ACTGGG	1 cut(s)	736
BmsI	GCATC	5 cut(s)	188, 308, 347, 1864, 2385
BmtI	GCTAGC	1 cut(s)	2613
BmuI	ACTGGG	1 cut(s)	736
BpiI	GAAGAC	2 cut(s)	256, 1988
BpmI	CTGGAG	2 cut(s)	1056, 2300
Bpu10I	CCTNAGC	1 cut(s)	1260
Bpu1102I	GCTNAGC	1 cut(s)	2239
BpuEI	CTTGAG	7 cut(s)	389, 674, 1157, 1922, 2288, 2435, 2548
BpuMI	CCSGG	3 cut(s)	520, 2201, 2538
BsaAI	YACGTR	1 cut(s)	1093
BsaJI	CCNNGG	9 cut(s)	459, 519, 1053, 1231, 1284, 1381, 1618, 1711, 1886
BsaWI	WCCGGW	5 cut(s)	182, 302, 1075, 1223, 2573
BsaXI	ACNNNNNCTCC	2 cut(s)	1029, 1059
Bsc4I	CCNNNNNNNGG	6 cut(s)	518, 519, 754, 1322, 1624, 2393
Bse118I	RCCGGY	2 cut(s)	347, 1223
Bse1I	ACTGG	3 cut(s)	742, 778, 1951
Bse3DI	GCAATG	4 cut(s)	1072, 2257, 2358, 2538
BseBI	CCWGG	4 cut(s)	727, 941, 1054, 1888
BseDI	CCNNGG	9 cut(s)	459, 519, 1053, 1231, 1284, 1381, 1618, 1711, 1886
BseGI	GGATG	8 cut(s)	166, 179, 299, 315, 338, 502, 1965, 2286
BseLI	CCNNNNNNNGG	6 cut(s)	518, 519, 754, 1322, 1624, 2393
BseMI	GCAATG	4 cut(s)	1072, 2257, 2358, 2538
BseMII	CTCAG	8 cut(s)	233, 401, 423, 437, 1043, 1566, 1725, 2334
BseNI	ACTGG	3 cut(s)	742, 778, 1951
BseX3I	CGGCCG	1 cut(s)	2596
BseXI	GCAGC	5 cut(s)	770, 1906, 2239, 2440, 2674
BseYI	CCCAGC	1 cut(s)	17
BsgI	GTGCAG	2 cut(s)	633, 2463
Bsh1236I	CGCG	1 cut(s)	1926
Bsh1285I	CGRYCG	2 cut(s)	307, 2599
BshFI	GGCC	7 cut(s)	144, 351, 871, 1450, 2204, 2386, 2598
BshNI	GGYRCC	1 cut(s)	1949
BshTI	ACCGGT	1 cut(s)	1223
BsiEI	CGRYCG	2 cut(s)	307, 2599
BsiHKAI	GWGCWC	3 cut(s)	2197, 2286, 2573
BsiHKCI	CYCGRG	1 cut(s)	1619
BslFI	GGGAC	2 cut(s)	68, 218
BslI	CCNNNNNNNGG	6 cut(s)	518, 519, 754, 1322, 1624, 2393
BsmAI	GTCTC	3 cut(s)	482, 1410, 2339
BsmBI	CGTCTC	1 cut(s)	482
BsmFI	GGGAC	2 cut(s)	68, 218
BsmI	GAATGC	2 cut(s)	535, 2274
BsnI	GGCC	7 cut(s)	144, 351, 871, 1450, 2204, 2386, 2598
BsoBI	CYCGRG	1 cut(s)	1619
Bsp1286I	GDGCHC	5 cut(s)	170, 2197, 2286, 2496, 2573
Bsp1407I	TGTACA	1 cut(s)	1860
Bsp143I	GATC	8 cut(s)	784, 860, 1126, 1276, 1578, 1812, 2110, 2184
Bsp1720I	GCTNAGC	1 cut(s)	2239
Bsp19I	CCATGG	1 cut(s)	1284
BspANI	GGCC	7 cut(s)	144, 351, 871, 1450, 2204, 2386, 2598
BspCNI	CTCAG	8 cut(s)	232, 400, 424, 436, 1042, 1567, 1726, 2335
BspFNI	CGCG	1 cut(s)	1926
BspHI	TCATGA	2 cut(s)	249, 603
BspMAI	CTGCAG	1 cut(s)	1498
BspMI	ACCTGC	2 cut(s)	1111, 1487
BspOI	GCTAGC	1 cut(s)	2613
BspPI	GGATC	6 cut(s)	779, 1134, 1271, 1807, 2105, 2118
BspQI	GCTCTTC	1 cut(s)	2484
BspT107I	GGYRCC	1 cut(s)	1949
BsrBI	CCGCTC	2 cut(s)	270, 2454
BsrDI	GCAATG	4 cut(s)	1072, 2257, 2358, 2538
BsrFI	RCCGGY	2 cut(s)	347, 1223
BsrGI	TGTACA	1 cut(s)	1860
BsrI	ACTGG	3 cut(s)	742, 778, 1951
BssAI	RCCGGY	2 cut(s)	347, 1223
BssECI	CCNNGG	9 cut(s)	459, 519, 1053, 1231, 1284, 1381, 1618, 1711, 1886
BssMI	GATC	8 cut(s)	784, 860, 1126, 1276, 1578, 1812, 2110, 2184
BssSI	CACGAG	1 cut(s)	2498
BssT1I	CCWWGG	5 cut(s)	459, 1231, 1284, 1381, 1711
Bst2BI	CACGAG	1 cut(s)	2498
Bst2UI	CCWGG	4 cut(s)	727, 941, 1054, 1888
Bst4CI	ACNGT	6 cut(s)	379, 584, 1704, 1805, 2083, 2507
Bst6I	CTCTTC	5 cut(s)	520, 1260, 1313, 2442, 2484
BstAPI	GCANNNNNTGC	3 cut(s)	344, 1502, 2659
BstAUI	TGTACA	1 cut(s)	1860
BstBAI	YACGTR	1 cut(s)	1093
BstC8I	GCNNGC	6 cut(s)	349, 1313, 1460, 1924, 2197, 2611
BstDSI	CCRYGG	1 cut(s)	1284
BstEII	GGTNACC	1 cut(s)	1226
BstF5I	GGATG	8 cut(s)	166, 179, 299, 315, 338, 502, 1965, 2286
BstFNI	CGCG	1 cut(s)	1926
BstHHI	GCGC	1 cut(s)	1700
BstKTI	GATC	8 cut(s)	787, 863, 1129, 1279, 1581, 1815, 2113, 2187
BstMAI	GTCTC	3 cut(s)	482, 1410, 2339
BstMBI	GATC	8 cut(s)	784, 860, 1126, 1276, 1578, 1812, 2110, 2184
BstMCI	CGRYCG	2 cut(s)	307, 2599
BstNI	CCWGG	4 cut(s)	727, 941, 1054, 1888
BstPI	GGTNACC	1 cut(s)	1226
BstSCI	CCNGG	7 cut(s)	518, 725, 939, 1052, 1886, 2199, 2536
BstSFI	CTRYAG	2 cut(s)	1494, 2432
BstUI	CGCG	1 cut(s)	1926
BstV1I	GCAGC	5 cut(s)	770, 1906, 2239, 2440, 2674
BstV2I	GAAGAC	2 cut(s)	256, 1988
BstX2I	RGATCY	3 cut(s)	1126, 2110, 2184
BstXI	CCANNNNNNTGG	2 cut(s)	865, 1458
BstYI	RGATCY	3 cut(s)	1126, 2110, 2184
BstZI	CGGCCG	1 cut(s)	2596
BsuI	GTATCC	1 cut(s)	1155
BsuRI	GGCC	7 cut(s)	144, 351, 871, 1450, 2204, 2386, 2598
BtgI	CCRYGG	1 cut(s)	1284
BtrI	CACGTC	1 cut(s)	2074
BtsCI	GGATG	8 cut(s)	166, 179, 299, 315, 338, 502, 1965, 2286
BtsI	GCAGTG	4 cut(s)	12, 381, 645, 852
BveI	ACCTGC	2 cut(s)	1111, 1487
Cac8I	GCNNGC	6 cut(s)	349, 1313, 1460, 1924, 2197, 2611
CaiI	CAGNNNCTG	2 cut(s)	236, 1502
CciI	TCATGA	2 cut(s)	249, 603
CfoI	GCGC	1 cut(s)	1700
Cfr10I	RCCGGY	2 cut(s)	347, 1223
Cfr13I	GGNCC	7 cut(s)	116, 223, 723, 869, 1448, 2051, 2521
CseI	GACGC	2 cut(s)	1439, 2602
CsiI	ACCWGGT	1 cut(s)	939
Csp6I	GTAC	5 cut(s)	597, 745, 937, 1861, 2119
CspAI	ACCGGT	1 cut(s)	1223
CspCI	CAANNNNNGTGG	2 cut(s)	634, 669
CviQI	GTAC	5 cut(s)	597, 745, 937, 1861, 2119
DpnI	GATC	8 cut(s)	786, 862, 1128, 1278, 1580, 1814, 2112, 2186
DpnII	GATC	8 cut(s)	784, 860, 1126, 1276, 1578, 1812, 2110, 2184
DraIII	CACNNNGTG	1 cut(s)	2639
DrdI	GACNNNNNNGTC	2 cut(s)	230, 2146
DseDI	GACNNNNNNGTC	2 cut(s)	230, 2146
EaeI	YGGCCR	1 cut(s)	2596
EagI	CGGCCG	1 cut(s)	2596
Eam1104I	CTCTTC	5 cut(s)	520, 1260, 1313, 2442, 2484
EarI	CTCTTC	5 cut(s)	520, 1260, 1313, 2442, 2484
EciI	GGCGGA	2 cut(s)	196, 2484
EclXI	CGGCCG	1 cut(s)	2596
Eco130I	CCWWGG	5 cut(s)	459, 1231, 1284, 1381, 1711
Eco24I	GRGCYC	2 cut(s)	170, 2496
Eco32I	GATATC	3 cut(s)	691, 1006, 1270
Eco47I	GGWCC	5 cut(s)	116, 223, 723, 2051, 2521
Eco52I	CGGCCG	1 cut(s)	2596
Eco57I	CTGAAG	3 cut(s)	213, 397, 2107
Eco88I	CYCGRG	1 cut(s)	1619
Eco91I	GGTNACC	1 cut(s)	1226
EcoO109I	RGGNCCY	4 cut(s)	223, 723, 2051, 2521
EcoO65I	GGTNACC	1 cut(s)	1226
EcoRI	GAATTC	3 cut(s)	275, 1071, 1724
EcoRII	CCWGG	4 cut(s)	725, 939, 1052, 1886
EcoRV	GATATC	3 cut(s)	691, 1006, 1270
EcoT14I	CCWWGG	5 cut(s)	459, 1231, 1284, 1381, 1711
EcoT38I	GRGCYC	2 cut(s)	170, 2496
ErhI	CCWWGG	5 cut(s)	459, 1231, 1284, 1381, 1711
Esp3I	CGTCTC	1 cut(s)	482
FaqI	GGGAC	2 cut(s)	68, 218
FblI	GTMKAC	4 cut(s)	1417, 1558, 2157, 2616
FokI	GGATG	8 cut(s)	166, 173, 286, 302, 325, 509, 1952, 2273
FriOI	GRGCYC	2 cut(s)	170, 2496
FspBI	CTAG	3 cut(s)	1155, 2606, 2610
GlaI	GCGC	1 cut(s)	1699
GsaI	CCCAGC	1 cut(s)	21
GsuI	CTGGAG	2 cut(s)	1056, 2300
HaeIII	GGCC	7 cut(s)	144, 351, 871, 1450, 2204, 2386, 2598
HgaI	GACGC	2 cut(s)	1439, 2602
HhaI	GCGC	1 cut(s)	1700
Hin6I	GCGC	1 cut(s)	1698
HinP1I	GCGC	1 cut(s)	1698
HincII	GTYRAC	2 cut(s)	1559, 2617
HindII	GTYRAC	2 cut(s)	1559, 2617
HindIII	AAGCTT	1 cut(s)	1754
HinfI	GANTC	7 cut(s)	134, 923, 930, 1114, 1178, 2561, 2618
HphI	GGTGA	2 cut(s)	271, 1664
Hpy166II	GTNNAC	6 cut(s)	1418, 1559, 1645, 2158, 2503, 2617
Hpy8I	GTNNAC	6 cut(s)	1418, 1559, 1645, 2158, 2503, 2617
Hpy99I	CGWCG	2 cut(s)	1416, 2618
HpyAV	CCTTC	7 cut(s)	237, 256, 976, 1420, 1550, 1554, 2512
HpyCH4III	ACNGT	6 cut(s)	379, 584, 1704, 1805, 2083, 2507
HpyCH4IV	ACGT	5 cut(s)	240, 491, 1092, 2073, 2674
HpySE526I	ACGT	5 cut(s)	240, 491, 1092, 2073, 2674
HspAI	GCGC	1 cut(s)	1698
KroI	GCCGGC	1 cut(s)	347
KroNI	GCCGGC	1 cut(s)	349
Kzo9I	GATC	8 cut(s)	784, 860, 1126, 1276, 1578, 1812, 2110, 2184
LguI	GCTCTTC	1 cut(s)	2484
LmnI	GCTCC	9 cut(s)	275, 628, 1037, 1467, 1916, 2227, 2281, 2568, 2601
Lsp1109I	GCAGC	5 cut(s)	770, 1906, 2239, 2440, 2674
LweI	GCATC	5 cut(s)	188, 308, 347, 1864, 2385
MabI	ACCWGGT	1 cut(s)	939
MaeI	CTAG	3 cut(s)	1155, 2606, 2610
MaeII	ACGT	5 cut(s)	240, 491, 1092, 2073, 2674
MaeIII	GTNAC	4 cut(s)	379, 769, 803, 1226
MalI	GATC	8 cut(s)	786, 862, 1128, 1278, 1580, 1814, 2112, 2186
MbiI	CCGCTC	2 cut(s)	270, 2454
MboI	GATC	8 cut(s)	784, 860, 1126, 1276, 1578, 1812, 2110, 2184
MflI	RGATCY	3 cut(s)	1126, 2110, 2184
MhlI	GDGCHC	5 cut(s)	170, 2197, 2286, 2496, 2573
MlyI	GAGTC	5 cut(s)	128, 917, 1123, 2555, 2612
MmeI	TCCRAC	1 cut(s)	474
MroNI	GCCGGC	1 cut(s)	347
MseI	TTAA	4 cut(s)	671, 987, 1212, 1242
MslI	CAYNNNNRTG	6 cut(s)	608, 957, 1947, 1974, 2084, 2576
MspR9I	CCNGG	7 cut(s)	520, 727, 941, 1054, 1888, 2201, 2538
Mva1269I	GAATGC	2 cut(s)	535, 2274
MvaI	CCWGG	4 cut(s)	727, 941, 1054, 1888
MvnI	CGCG	1 cut(s)	1926
NaeI	GCCGGC	1 cut(s)	349
NciI	CCSGG	3 cut(s)	520, 2201, 2538
NcoI	CCATGG	1 cut(s)	1284
NdeII	GATC	8 cut(s)	784, 860, 1126, 1276, 1578, 1812, 2110, 2184
NgoMIV	GCCGGC	1 cut(s)	347
NheI	GCTAGC	1 cut(s)	2609
NmuCI	GTSAC	1 cut(s)	379
OliI	CACNNNNGTG	2 cut(s)	1947, 2084
PagI	TCATGA	2 cut(s)	249, 603
PaqCI	CACCTGC	1 cut(s)	1111
PciSI	GCTCTTC	1 cut(s)	2484
PctI	GAATGC	2 cut(s)	535, 2274
PdiI	GCCGGC	1 cut(s)	349
PfeI	GAWTC	2 cut(s)	930, 1178
PinAI	ACCGGT	1 cut(s)	1223
PleI	GAGTC	5 cut(s)	128, 917, 1122, 2555, 2612
PpsI	GAGTC	5 cut(s)	128, 917, 1122, 2555, 2612
Ppu21I	YACGTR	1 cut(s)	1093
PpuMI	RGGWCCY	4 cut(s)	223, 723, 2051, 2521
PshBI	ATTAAT	1 cut(s)	1212
Psp5II	RGGWCCY	4 cut(s)	223, 723, 2051, 2521
Psp6I	CCWGG	4 cut(s)	725, 939, 1052, 1886
PspEI	GGTNACC	1 cut(s)	1226
PspFI	CCCAGC	1 cut(s)	17
PspGI	CCWGG	4 cut(s)	725, 939, 1052, 1886
PspPI	GGNCC	7 cut(s)	116, 223, 723, 869, 1448, 2051, 2521
PspPPI	RGGWCCY	4 cut(s)	223, 723, 2051, 2521
PstI	CTGCAG	1 cut(s)	1498
PstNI	CAGNNNCTG	2 cut(s)	236, 1502
PsuI	RGATCY	3 cut(s)	1126, 2110, 2184
RsaI	GTAC	5 cut(s)	598, 746, 938, 1862, 2120
RsaNI	GTAC	5 cut(s)	597, 745, 937, 1861, 2119
RseI	CAYNNNNRTG	6 cut(s)	608, 957, 1947, 1974, 2084, 2576
SalI	GTCGAC	2 cut(s)	1557, 2615
SapI	GCTCTTC	1 cut(s)	2484
SaqAI	TTAA	4 cut(s)	671, 987, 1212, 1242
Sau3AI	GATC	8 cut(s)	784, 860, 1126, 1276, 1578, 1812, 2110, 2184
Sau96I	GGNCC	7 cut(s)	116, 223, 723, 869, 1448, 2051, 2521
ScaI	AGTACT	1 cut(s)	2120
SchI	GAGTC	5 cut(s)	128, 917, 1123, 2555, 2612
ScrFI	CCNGG	7 cut(s)	520, 727, 941, 1054, 1888, 2201, 2538
SduI	GDGCHC	5 cut(s)	170, 2197, 2286, 2496, 2573
SexAI	ACCWGGT	1 cut(s)	939
SfaNI	GCATC	5 cut(s)	188, 308, 347, 1864, 2385
SfcI	CTRYAG	2 cut(s)	1494, 2432
SinI	GGWCC	5 cut(s)	116, 223, 723, 2051, 2521
SmiMI	CAYNNNNRTG	6 cut(s)	608, 957, 1947, 1974, 2084, 2576
SmlI	CTYRAG	7 cut(s)	404, 653, 1136, 1901, 2303, 2414, 2563
SmoI	CTYRAG	7 cut(s)	404, 653, 1136, 1901, 2303, 2414, 2563
SpeI	ACTAGT	1 cut(s)	1154
SspI	AATATT	1 cut(s)	1993
SspMI	CTAG	3 cut(s)	1155, 2606, 2610
StyD4I	CCNGG	7 cut(s)	518, 725, 939, 1052, 1886, 2199, 2536
StyI	CCWWGG	5 cut(s)	459, 1231, 1284, 1381, 1711
TaaI	ACNGT	6 cut(s)	379, 584, 1704, 1805, 2083, 2507
TaiI	ACGT	5 cut(s)	243, 494, 1095, 2076, 2677
TaqI	TCGA	9 cut(s)	307, 480, 921, 1002, 1558, 2181, 2292, 2484, 2616
TaqII	GACCGA	1 cut(s)	2156
TatI	WGTACW	2 cut(s)	1860, 2118
TauI	GCSGC	6 cut(s)	212, 215, 700, 2131, 2457, 2598
TfiI	GAWTC	2 cut(s)	930, 1178
Tru1I	TTAA	4 cut(s)	671, 987, 1212, 1242
Tru9I	TTAA	4 cut(s)	671, 987, 1212, 1242
TseFI	GTSAC	1 cut(s)	379
TseI	GCWGC	5 cut(s)	758, 1919, 2252, 2428, 2662
Tsp45I	GTSAC	1 cut(s)	379
TspDTI	ATGAA	5 cut(s)	23, 238, 304, 1737, 2369
TspGWI	ACGGA	4 cut(s)	308, 1206, 1438, 1854
VpaK11BI	GGWCC	5 cut(s)	116, 223, 723, 2051, 2521
VspI	ATTAAT	1 cut(s)	1212
XapI	RAATTY	5 cut(s)	190, 275, 287, 1071, 1724
XmiI	GTMKAC	4 cut(s)	1417, 1558, 2157, 2616
XspI	CTAG	3 cut(s)	1155, 2606, 2610
ZrmI	AGTACT	1 cut(s)	2120

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.