MD04G1177000.v1.1

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016531
Physical Location & Seq
Forward (+)
26835265 .. 26837405
2141 bp
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UTR
Exon/CDS
Intron
MD04G1177000.v1.1.491

Sequence Viewer

Length: 681 bp
ATGAACGATGCAGATGTGTCGAAGCAGATTCAGCAGATGGTCAGATTCATTCGCCAAGAGGCCGAAGAGAAAGCCAATGAGATTTCTGTCTCTGCTGAGGAGGAGTTTAACATTGAGAAACTGCAAATAGTTGAAGCTGAAAAGAAAAAGATCAGACAAGAGTATGACCGCAAGGCAAAGCAGGTGGACGTCAAAAAGAAAATAGAATACTCGATGCAATTGAACGCGTCGCGTATAAAAGTTCTTCAAGCGCAAGATGACATTGTAAATTCCATGAAAGAATCTGCTGGCAAGGATCTTCTCAAAGTTGCTGATGGCAAGAAGGCATACAAGAAGCTCCTCAAAGACTTGATTGTTCAGAGTTTACTGCGGCTAAAGGAGCCAGCAGTGTTGCTGAGGTGCAGAGAGGTTGACAAAAAGGTTGTTGAGTCTGTGTTGGAGGAAGCAAAGAAAGCTTACGCTGATAAAGCCAATGTTCCTGCCCCCAAAATCACCATCGACGATCGTGTGTTTCTCCCACCTCCTCCGAAGAGTGGCGATTCCCACGAACCCTATTGTTCTGGTGGAGTGGTGGTGGCCTCCCAAGACGGGAAGATTGTTTGCGAAAACACCCTTGATGCGCGGTTGGATGTCGTGTACAGACAGAAACTGCCTGAGATTCGGAAGCGCCTTTTAGGTTAA

Protein Analysis

227

Amino Acids

25.63

Weight (kDa)

9.02

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
vATP-synt_E PF01991 16 - 224 3.5e-65 ATP synthase (E/31 kDa) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016531)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G08560
fragaria_vesca FvH4_6g09500
malus_domestica MD04G1177000.v1.1 MD12G1192200.v1.1
prunus_persica Prupe.6G296700_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0460081
rosa_laevigata RLG00000025045
rosa_multiflora Rmu_sc0000449.1_g000055
rosa_roxburghii Rroxscaffold_6G00419860
rosa_rugosa Rorug03G0039300
rosa_samantha Rh3BG100900 Rh3CG103000 Rh3DG102200
rosa_wichuraiana Rw3G008250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 172
AatII GACGTC 1 cut(s) 192
Acc36I ACCTGC 1 cut(s) 172
AccII CGCG 3 cut(s) 227, 232, 622
AciI CCGC 3 cut(s) 169, 370, 622
AclWI GGATC 1 cut(s) 303
AcsI RAATTY 1 cut(s) 268
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 1 cut(s) 638
AfiI CCNNNNNNNGG 2 cut(s) 533, 588
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 3 cut(s) 134, 223, 248
AluBI AGCT 3 cut(s) 137, 337, 455
AluI AGCT 3 cut(s) 137, 337, 455
Alw26I GTCTC 1 cut(s) 94
AlwI GGATC 1 cut(s) 303
AlwNI CAGNNNCTG 1 cut(s) 649
AoxI GGCC 2 cut(s) 60, 576
ApoI RAATTY 1 cut(s) 268
AspLEI GCGC 3 cut(s) 253, 622, 669
AsuHPI GGTGA 1 cut(s) 484
BbvCI CCTCAGC 2 cut(s) 96, 395
BccI CCATC 3 cut(s) 31, 308, 503
BcgI CGANNNNNNTGC 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 94
BfoI RGCGCY 1 cut(s) 670
BfuAI ACCTGC 1 cut(s) 172
BisI GCNGC 1 cut(s) 371
BlsI GCNGC 1 cut(s) 372
BmiI GGNNCC 1 cut(s) 381
BmsI GCATC 2 cut(s) 204, 607
Bpu10I CCTNAGC 2 cut(s) 96, 395
BsaHI GRCGYC 1 cut(s) 189
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 2 cut(s) 533, 588
BseGI GGATG 1 cut(s) 634
BseLI CCNNNNNNNGG 2 cut(s) 533, 588
BseMII CTCAG 3 cut(s) 87, 386, 645
BseRI GAGGAG 4 cut(s) 113, 116, 329, 513
BsgI GTGCAG 1 cut(s) 421
Bsh1236I CGCG 3 cut(s) 227, 232, 622
Bsh1285I CGRYCG 1 cut(s) 505
BshFI GGCC 2 cut(s) 62, 578
BsiEI CGRYCG 1 cut(s) 505
BslI CCNNNNNNNGG 2 cut(s) 533, 588
BsmAI GTCTC 1 cut(s) 94
BsnI GGCC 2 cut(s) 62, 578
Bsp1407I TGTACA 1 cut(s) 636
Bsp143I GATC 3 cut(s) 150, 295, 502
BspACI CCGC 3 cut(s) 169, 370, 622
BspANI GGCC 2 cut(s) 62, 578
BspCNI CTCAG 3 cut(s) 88, 387, 646
BspFNI CGCG 3 cut(s) 227, 232, 622
BspLI GGNNCC 1 cut(s) 381
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 1 cut(s) 303
BsrGI TGTACA 1 cut(s) 636
BssMI GATC 3 cut(s) 150, 295, 502
BssNI GRCGYC 1 cut(s) 189
Bst6I CTCTTC 2 cut(s) 60, 524
BstACI GRCGYC 1 cut(s) 189
BstAUI TGTACA 1 cut(s) 636
BstC8I GCNNGC 2 cut(s) 289, 384
BstDEI CTNAG 3 cut(s) 96, 395, 654
BstF5I GGATG 1 cut(s) 634
BstFNI CGCG 3 cut(s) 227, 232, 622
BstH2I RGCGCY 1 cut(s) 670
BstHHI GCGC 3 cut(s) 253, 622, 669
BstKTI GATC 3 cut(s) 153, 298, 505
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 3 cut(s) 150, 295, 502
BstMCI CGRYCG 1 cut(s) 505
BstMWI GCNNNNNNNGC 4 cut(s) 31, 379, 452, 467
BstUI CGCG 3 cut(s) 227, 232, 622
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BsuRI GGCC 2 cut(s) 62, 578
BtsCI GGATG 1 cut(s) 634
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 1 cut(s) 393
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 2 cut(s) 289, 384
CaiI CAGNNNCTG 1 cut(s) 649
CfoI GCGC 3 cut(s) 253, 622, 669
CseI GACGC 1 cut(s) 216
Csp6I GTAC 1 cut(s) 637
CspCI CAANNNNNGTGG 2 cut(s) 165, 200
CviAII CATG 1 cut(s) 274
CviJI RGCY 9 cut(s) 62, 74, 137, 337, 373, 382, 455, 470, 578
CviKI_1 RGCY 9 cut(s) 62, 74, 137, 337, 373, 382, 455, 470, 578
CviQI GTAC 1 cut(s) 637
DdeI CTNAG 3 cut(s) 96, 395, 654
DpnI GATC 3 cut(s) 152, 297, 504
DpnII GATC 3 cut(s) 150, 295, 502
Eam1104I CTCTTC 2 cut(s) 60, 524
EarI CTCTTC 2 cut(s) 60, 524
FaeI CATG 1 cut(s) 277
FaiI YATR 4 cut(s) 165, 236, 275, 328
FatI CATG 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 371
FokI GGATG 1 cut(s) 641
Fsp4HI GCNGC 1 cut(s) 371
GlaI GCGC 3 cut(s) 252, 621, 668
GluI GCNGC 1 cut(s) 371
HaeII RGCGCY 1 cut(s) 670
HaeIII GGCC 2 cut(s) 62, 578
HgaI GACGC 1 cut(s) 216
HhaI GCGC 3 cut(s) 253, 622, 669
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 1 cut(s) 277
Hin6I GCGC 3 cut(s) 251, 620, 667
HinP1I GCGC 3 cut(s) 251, 620, 667
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HindIII AAGCTT 1 cut(s) 453
HinfI GANTC 6 cut(s) 28, 45, 281, 428, 539, 658
HphI GGTGA 1 cut(s) 484
Hpy166II GTNNAC 4 cut(s) 187, 365, 412, 637
Hpy188I TCNGA 5 cut(s) 44, 155, 360, 528, 663
Hpy8I GTNNAC 4 cut(s) 187, 365, 412, 637
Hpy99I CGWCG 2 cut(s) 232, 503
HpyAV CCTTC 1 cut(s) 316
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 4 cut(s) 11, 124, 217, 402
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 379, 452, 467
HpyF3I CTNAG 3 cut(s) 96, 395, 654
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 1 cut(s) 277
HspAI GCGC 3 cut(s) 251, 620, 667
Kzo9I GATC 3 cut(s) 150, 295, 502
LmnI GCTCC 2 cut(s) 342, 379
LpnPI CCDG 6 cut(s) 167, 273, 396, 492, 546, 666
LweI GCATC 2 cut(s) 204, 607
MaeII ACGT 1 cut(s) 189
MalI GATC 3 cut(s) 152, 297, 504
MboI GATC 3 cut(s) 150, 295, 502
MboII GAAGA 5 cut(s) 77, 236, 290, 541, 604
MfeI CAATTG 1 cut(s) 218
MflI RGATCY 1 cut(s) 295
MluCI AATT 2 cut(s) 218, 268
MluI ACGCGT 1 cut(s) 225
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 2 cut(s) 417, 606
MseI TTAA 2 cut(s) 108, 679
MunI CAATTG 1 cut(s) 218
MvnI CGCG 3 cut(s) 227, 232, 622
MwoI GCNNNNNNNGC 4 cut(s) 31, 379, 452, 467
NdeII GATC 3 cut(s) 150, 295, 502
NlaIII CATG 1 cut(s) 277
NlaIV GGNNCC 1 cut(s) 381
PaqCI CACCTGC 1 cut(s) 172
PfeI GAWTC 5 cut(s) 28, 45, 281, 539, 658
PkrI GCNGC 1 cut(s) 372
Ple19I CGATCG 1 cut(s) 505
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
PspN4I GGNNCC 1 cut(s) 381
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 295
PvuI CGATCG 1 cut(s) 505
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
SaqAI TTAA 2 cut(s) 108, 679
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 3 cut(s) 150, 295, 502
SchI GAGTC 1 cut(s) 437
SfaNI GCATC 2 cut(s) 204, 607
Sse9I AATT 2 cut(s) 218, 268
SsiI CCGC 3 cut(s) 169, 370, 622
TaiI ACGT 1 cut(s) 192
TaqI TCGA 3 cut(s) 20, 212, 498
TasI AATT 2 cut(s) 218, 268
TatI WGTACW 1 cut(s) 636
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 5 cut(s) 28, 45, 281, 539, 658
Tru1I TTAA 2 cut(s) 108, 679
Tru9I TTAA 2 cut(s) 108, 679
TscAI CASTG 1 cut(s) 393
TspDTI ATGAA 3 cut(s) 17, 37, 290
TspRI CASTG 1 cut(s) 393
XapI RAATTY 1 cut(s) 268
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.