MD12G1192200.v1.1

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016531
Physical Location & Seq
Forward (+)
27417166 .. 27418992
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1192200.v1.1.491

Sequence Viewer

Length: 744 bp
ATGAACGATGCAGATGTGTCGAAGCAGATTCAGCAAATGGTCAGATTCATTCGGCAAGAGGCCGAAGAGAAAGCCAATGAGATTTCTGTCTCTGCTGAGGAGGAATTTAACATTGAGAAACTGCAATTGGTTGAAGCTGAAAAGAAAAAGATCAGACAAGAGTATGACCGCAAGGCAAAGCAGATGGACGTCAAGAAGAAAATCGAATACTCGATGCAACTGAACGCGTCGCGTATAAAAGTTCTTCAAGCACAAGATGACATTGTAAATTCCATGAAAGAATCTGCTGGCAAGGATCTTCTCAAAGTTGCTAATGACAAGAAGGCATACAAGAATCTCATCAAAGACTTAATTGTTCAGAGTTTACTGCGGCTAAAGGAACCGGCAGTGTTGCTGAGGTGCAGAGAGGTTGACAAAAAGGTTGTTGAGTCTGTGTTGGAGGAAGCAAAGAAAGCTTATGCTGATAAAGCCAATGTTTCCGGCCCCAAAGTCACCGTCGATGGTCGTGTGTTTCTCCCACCTCCTCCGAAGGGCGGAGATTCCCACGAACCCTATTGTTCTGGCGGAGTGGTGGTGGCCTCCCAAGACGGGAAGATTGTGTGCGAAAACACCCTTGATGCGCGATTGGATGTCGTGTACAGACAGAAACTGCCTGAGGTAAGGCCAAGCAAACTTTGTACTCCATTTGGACATTCATTCAGCACACCAGAATCAAAACAAAACTCAAATCTTTACTTTGCATGA

Protein Analysis

248

Amino Acids

27.85

Weight (kDa)

8.83

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
vATP-synt_E PF01991 16 - 221 2.1e-64 ATP synthase (E/31 kDa) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016531)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G08560
fragaria_vesca FvH4_6g09500
malus_domestica MD04G1177000.v1.1 MD12G1192200.v1.1
prunus_persica Prupe.6G296700_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0460081
rosa_laevigata RLG00000025045
rosa_multiflora Rmu_sc0000449.1_g000055
rosa_roxburghii Rroxscaffold_6G00419860
rosa_rugosa Rorug03G0039300
rosa_samantha Rh3BG100900 Rh3CG103000 Rh3DG102200
rosa_wichuraiana Rw3G008250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 192
AccII CGCG 3 cut(s) 227, 232, 622
AciI CCGC 4 cut(s) 169, 370, 534, 564
AclWI GGATC 1 cut(s) 303
AcsI RAATTY 2 cut(s) 104, 268
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 2 cut(s) 638, 679
AfiI CCNNNNNNNGG 3 cut(s) 530, 533, 588
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 2 cut(s) 134, 248
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 2 cut(s) 137, 455
AluI AGCT 2 cut(s) 137, 455
Alw26I GTCTC 1 cut(s) 94
AlwI GGATC 1 cut(s) 303
AlwNI CAGNNNCTG 1 cut(s) 649
AoxI GGCC 4 cut(s) 60, 481, 576, 662
ApoI RAATTY 2 cut(s) 104, 268
ArsI GACNNNNNNTTYG 2 cut(s) 480, 512
AspLEI GCGC 1 cut(s) 622
AspS9I GGNCC 1 cut(s) 482
AsuHPI GGTGA 1 cut(s) 484
AxyI CCTNAGG 1 cut(s) 654
BbvCI CCTCAGC 2 cut(s) 96, 395
BccI CCATC 2 cut(s) 178, 494
BcgI CGANNNNNNTGC 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 94
BisI GCNGC 1 cut(s) 371
BlsI GCNGC 1 cut(s) 372
BmgT120I GGNCC 1 cut(s) 482
BmiI GGNNCC 2 cut(s) 381, 484
BmsI GCATC 2 cut(s) 204, 607
Bpu10I CCTNAGC 2 cut(s) 96, 395
BsaHI GRCGYC 1 cut(s) 189
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 3 cut(s) 530, 533, 588
Bse118I RCCGGY 1 cut(s) 382
Bse21I CCTNAGG 1 cut(s) 654
BseGI GGATG 1 cut(s) 634
BseLI CCNNNNNNNGG 3 cut(s) 530, 533, 588
BseMII CTCAG 3 cut(s) 87, 386, 645
BseRI GAGGAG 2 cut(s) 113, 513
BsgI GTGCAG 1 cut(s) 421
Bsh1236I CGCG 3 cut(s) 227, 232, 622
BshFI GGCC 4 cut(s) 62, 483, 578, 664
BsiSI CCGG 2 cut(s) 383, 480
BslI CCNNNNNNNGG 3 cut(s) 530, 533, 588
BsmAI GTCTC 1 cut(s) 94
BsnI GGCC 4 cut(s) 62, 483, 578, 664
Bsp1407I TGTACA 1 cut(s) 636
Bsp143I GATC 2 cut(s) 150, 295
BspACI CCGC 4 cut(s) 169, 370, 534, 564
BspANI GGCC 4 cut(s) 62, 483, 578, 664
BspCNI CTCAG 3 cut(s) 88, 387, 646
BspFNI CGCG 3 cut(s) 227, 232, 622
BspLI GGNNCC 2 cut(s) 381, 484
BspPI GGATC 1 cut(s) 303
BsrFI RCCGGY 1 cut(s) 382
BsrGI TGTACA 1 cut(s) 636
BssAI RCCGGY 1 cut(s) 382
BssMI GATC 2 cut(s) 150, 295
BssNI GRCGYC 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 496
Bst6I CTCTTC 1 cut(s) 60
BstACI GRCGYC 1 cut(s) 189
BstAUI TGTACA 1 cut(s) 636
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 3 cut(s) 96, 395, 654
BstF5I GGATG 1 cut(s) 634
BstFNI CGCG 3 cut(s) 227, 232, 622
BstHHI GCGC 1 cut(s) 622
BstKTI GATC 2 cut(s) 153, 298
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 2 cut(s) 150, 295
BstMWI GCNNNNNNNGC 3 cut(s) 31, 452, 467
BstUI CGCG 3 cut(s) 227, 232, 622
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
Bsu36I CCTNAGG 1 cut(s) 654
BsuRI GGCC 4 cut(s) 62, 483, 578, 664
BtsCI GGATG 1 cut(s) 634
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 1 cut(s) 393
Cac8I GCNNGC 1 cut(s) 289
CaiI CAGNNNCTG 1 cut(s) 649
CfoI GCGC 1 cut(s) 622
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 482
CseI GACGC 1 cut(s) 216
Csp6I GTAC 2 cut(s) 637, 678
CviAII CATG 2 cut(s) 274, 741
CviJI RGCY 9 cut(s) 62, 74, 137, 373, 455, 470, 483, 578, 664
CviKI_1 RGCY 9 cut(s) 62, 74, 137, 373, 455, 470, 483, 578, 664
CviQI GTAC 2 cut(s) 637, 678
DdeI CTNAG 3 cut(s) 96, 395, 654
DpnI GATC 2 cut(s) 152, 297
DpnII GATC 2 cut(s) 150, 295
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
EciI GGCGGA 2 cut(s) 549, 579
Eco81I CCTNAGG 1 cut(s) 654
FaeI CATG 2 cut(s) 277, 744
FaiI YATR 6 cut(s) 165, 236, 275, 328, 459, 742
FatI CATG 2 cut(s) 273, 740
Fnu4HI GCNGC 1 cut(s) 371
FokI GGATG 1 cut(s) 641
Fsp4HI GCNGC 1 cut(s) 371
GlaI GCGC 1 cut(s) 621
GluI GCNGC 1 cut(s) 371
HaeIII GGCC 4 cut(s) 62, 483, 578, 664
HapII CCGG 2 cut(s) 383, 480
HgaI GACGC 1 cut(s) 216
HhaI GCGC 1 cut(s) 622
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 2 cut(s) 277, 744
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HindIII AAGCTT 1 cut(s) 453
HinfI GANTC 7 cut(s) 28, 45, 281, 334, 428, 539, 710
HpaII CCGG 2 cut(s) 383, 480
HphI GGTGA 1 cut(s) 484
Hpy166II GTNNAC 3 cut(s) 365, 412, 637
Hpy188I TCNGA 4 cut(s) 44, 155, 360, 528
Hpy188III TCNNGA 1 cut(s) 193
Hpy8I GTNNAC 3 cut(s) 365, 412, 637
Hpy99I CGWCG 2 cut(s) 232, 500
HpyAV CCTTC 2 cut(s) 316, 523
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 5 cut(s) 11, 124, 217, 402, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 452, 467
HpyF3I CTNAG 3 cut(s) 96, 395, 654
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 2 cut(s) 277, 744
HspAI GCGC 1 cut(s) 620
Kzo9I GATC 2 cut(s) 150, 295
LpnPI CCDG 6 cut(s) 273, 396, 493, 546, 666, 720
LweI GCATC 2 cut(s) 204, 607
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 490
MalI GATC 2 cut(s) 152, 297
MboI GATC 2 cut(s) 150, 295
MboII GAAGA 5 cut(s) 77, 208, 236, 290, 604
MfeI CAATTG 1 cut(s) 125
MflI RGATCY 1 cut(s) 295
MluCI AATT 4 cut(s) 104, 125, 268, 351
MluI ACGCGT 1 cut(s) 225
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 1 cut(s) 417
MseI TTAA 2 cut(s) 108, 350
MspI CCGG 2 cut(s) 383, 480
MunI CAATTG 1 cut(s) 125
MvnI CGCG 3 cut(s) 227, 232, 622
MwoI GCNNNNNNNGC 3 cut(s) 31, 452, 467
NdeII GATC 2 cut(s) 150, 295
NlaIII CATG 2 cut(s) 277, 744
NlaIV GGNNCC 2 cut(s) 381, 484
NmuCI GTSAC 1 cut(s) 490
PfeI GAWTC 6 cut(s) 28, 45, 281, 334, 539, 710
PkrI GCNGC 1 cut(s) 372
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 381, 484
PspPI GGNCC 1 cut(s) 482
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 2 cut(s) 638, 679
RsaNI GTAC 2 cut(s) 637, 678
SaqAI TTAA 2 cut(s) 108, 350
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 2 cut(s) 150, 295
Sau96I GGNCC 1 cut(s) 482
SchI GAGTC 1 cut(s) 437
SetI ASST 8 cut(s) 139, 192, 401, 411, 423, 457, 523, 660
SfaNI GCATC 2 cut(s) 204, 607
Sse9I AATT 4 cut(s) 104, 125, 268, 351
SsiI CCGC 4 cut(s) 169, 370, 534, 564
TaaI ACNGT 1 cut(s) 496
TaiI ACGT 1 cut(s) 192
TaqI TCGA 4 cut(s) 20, 204, 212, 498
TasI AATT 4 cut(s) 104, 125, 268, 351
TatI WGTACW 2 cut(s) 636, 677
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 6 cut(s) 28, 45, 281, 334, 539, 710
Tru1I TTAA 2 cut(s) 108, 350
Tru9I TTAA 2 cut(s) 108, 350
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 490
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 4 cut(s) 17, 37, 290, 684
TspRI CASTG 1 cut(s) 393
XapI RAATTY 2 cut(s) 104, 268
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.