MD04G1190200.v1.1

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0019773
Physical Location & Seq
Forward (+)
28023367 .. 28025727
2361 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1190200.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGTGTGTTGTATACCAAGGTAACTTATCTAAAGTACTCCAAGTGCTTACCAATATCATGGCTCTACTCCTCACAAACCAAGGCCAATCCCTCGCCTTATTTCTGGTTCTCGTTTTGGGCTTCTGCTCCTCCCAAGCTTTTTGTAGTCGTCAACTTTCTGATGACGAAAAAATCATGTTGGAGAGGCACAAGGAGTGGATGGCTCGACATGGACATGTTTACAAGGATGCACAAGAGAAGGACAGGCGTTACAACACATTCAAGTCCAATGTGGAGTTTATAAAAGGCTTTAACAAGAAGAGCGAAAACAAGTACAGGCTCGACATCAATAAGTTTGCAGACCTAACTAACGAGGAATTTCAGGCGATGAGTCAAGGCTACAAGAGGATACACCTCCCCGAGATCATGTCCAACTCTTCCAAAGCAGCTCCAGATAACAATTTTCGATATGAAAATGTCACCGATGTGCCAATATCTAAGGATTATTTTTCAATTGGCGCGGTGACCACAGTCAAATACCAAGGGCAATGTGGATGTTGTTGGGCGTTCGCATCAGTGGCAACCGTAGAAGGGCTAAACAAACTCAAAACAGGAAACCTAATTTCACTATCAGAACAAGAGCTAATTGATTGTGACACGAACAGCTACGATCACGGCTGCGATGGAGGTAATTCAAAGGTTGCGTATGACTACATGATCAAACTCAATAAAAGCCTTGCAACTGAAGATAATTACCCTTACCAGGCTAAAGATGGAGGAATTTGCAGAACTACTACTGTTGCTCCTGCATCTGGTGCCATAACCATAACCGGCTACGAGAATGTGCCTGCAAACAATGAAGATGCATTGTTGAAGGCTGTTGCGAACCAGCCGGTTTCTGTTGCCATTGATATTAACGCAAAGGAATTCAAGTATTATTCGAGTGGCGTGTACACTGGACCATGTGGTACAAACTTAACCCATGAAGTGACTGCAGTTGGGTACGGTACTAGTAATGATGGGACTAAGTATTGGCTGCTCAAGAATTCTTGGGGTAAAGAGTGGGGGGAGAGTGGATACCTTAGATTACAAAGAGATGTGCCCGCCAAGGAAGGTCTATGTGGCATTGCTATGGAAGCTACCTATCCAACCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009791 GO:0009814 GO:0009817 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010150 GO:0010224 GO:0010282 GO:0010623 GO:0012501 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0034284 GO:0034285 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0046688 GO:0048366 GO:0048367 GO:0048437 GO:0048468 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0071704 GO:0080187 GO:0090567 GO:0090693 GO:0097501 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1990169
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

379

Amino Acids

41.95

Weight (kDa)

6.12

Isoelectric Point (pI)

29.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 63 - 120 8.3e-17 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 161 - 377 6.6e-71 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019773)

Species Orthologous Gene IDs
malus_domestica MD04G1190100.v1.1 MD04G1190200.v1.1 MD12G1202900.v1.1 MD12G1203000.v1.1
prunus_persica Prupe.4G153800_v2.0.a1
pyrus_communis pycom04g16840 pycom12g18940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 794
AccI GTMKAC 1 cut(s) 12
AccII CGCG 1 cut(s) 500
AciI CCGC 3 cut(s) 500, 1083, 1131
AcsI RAATTY 4 cut(s) 356, 759, 905, 1024
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 6 cut(s) 36, 314, 932, 949, 983, 988
AfiI CCNNNNNNNGG 3 cut(s) 570, 742, 791
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 5 cut(s) 262, 492, 675, 853, 910
AhlI ACTAGT 1 cut(s) 989
AjnI CCWGG 1 cut(s) 741
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 5 cut(s) 137, 428, 622, 645, 1118
AluI AGCT 5 cut(s) 137, 428, 622, 645, 1118
Ama87I CYCGRG 1 cut(s) 398
AoxI GGCC 1 cut(s) 82
ApeKI GCWGC 3 cut(s) 425, 657, 1015
ApoI RAATTY 4 cut(s) 356, 759, 905, 1024
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 1 cut(s) 938
AsuHPI GGTGA 2 cut(s) 451, 514
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 1 cut(s) 938
BaeGI GKGCMC 1 cut(s) 1083
BanI GGYRCC 1 cut(s) 794
BbvI GCAGC 3 cut(s) 437, 644, 1002
BccI CCATC 4 cut(s) 193, 656, 746, 992
BceAI ACGGC 1 cut(s) 670
BciT130I CCWGG 1 cut(s) 743
BciVI GTATCC 2 cut(s) 381, 1049
BclI TGATCA 1 cut(s) 696
BcuI ACTAGT 1 cut(s) 989
BfaI CTAG 1 cut(s) 990
BfmI CTRYAG 1 cut(s) 972
BfuI GTATCC 2 cut(s) 381, 1049
BisI GCNGC 3 cut(s) 426, 658, 1016
BlsI GCNGC 3 cut(s) 427, 659, 1017
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 743
Bme18I GGWCC 1 cut(s) 938
BmeT110I CYCGRG 1 cut(s) 398
BmgT120I GGNCC 1 cut(s) 938
BmiI GGNNCC 1 cut(s) 796
BmrFI CCNGG 1 cut(s) 743
BmsI GCATC 4 cut(s) 217, 560, 797, 832
BoxI GACNNNNGTC 1 cut(s) 509
BpmI CTGGAG 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 1004
BsaJI CCNNGG 4 cut(s) 16, 79, 520, 1086
BsaXI ACNNNNNCTCC 2 cut(s) 766, 796
Bsc4I CCNNNNNNNGG 3 cut(s) 570, 742, 791
Bse118I RCCGGY 2 cut(s) 809, 871
Bse1I ACTGG 1 cut(s) 940
Bse3DI GCAATG 2 cut(s) 533, 1104
BseBI CCWGG 1 cut(s) 743
BseDI CCNNGG 4 cut(s) 16, 79, 520, 1086
BseGI GGATG 3 cut(s) 204, 232, 539
BseLI CCNNNNNNNGG 3 cut(s) 570, 742, 791
BseMI GCAATG 2 cut(s) 533, 1104
BseNI ACTGG 1 cut(s) 940
BseRI GAGGAG 2 cut(s) 59, 118
BseSI GKGCMC 1 cut(s) 1083
BseXI GCAGC 3 cut(s) 437, 644, 1002
Bsh1236I CGCG 1 cut(s) 500
BshFI GGCC 1 cut(s) 84
BshNI GGYRCC 1 cut(s) 794
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 2 cut(s) 810, 872
BslFI GGGAC 1 cut(s) 1015
BslI CCNNNNNNNGG 3 cut(s) 570, 742, 791
BsmFI GGGAC 1 cut(s) 1015
BsnI GGCC 1 cut(s) 84
BsoBI CYCGRG 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 1083
Bsp1407I TGTACA 1 cut(s) 930
Bsp143I GATC 3 cut(s) 402, 649, 696
BspACI CCGC 3 cut(s) 500, 1083, 1131
BspANI GGCC 1 cut(s) 84
BspFNI CGCG 1 cut(s) 500
BspLI GGNNCC 1 cut(s) 796
BspMAI CTGCAG 1 cut(s) 976
BspQI GCTCTTC 1 cut(s) 293
BspT107I GGYRCC 1 cut(s) 794
BsrDI GCAATG 2 cut(s) 533, 1104
BsrFI RCCGGY 2 cut(s) 809, 871
BsrGI TGTACA 1 cut(s) 930
BsrI ACTGG 1 cut(s) 940
BssAI RCCGGY 2 cut(s) 809, 871
BssECI CCNNGG 4 cut(s) 16, 79, 520, 1086
BssMI GATC 3 cut(s) 402, 649, 696
BssNAI GTATAC 1 cut(s) 13
BssT1I CCWWGG 4 cut(s) 16, 79, 520, 1086
Bst1107I GTATAC 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 743
Bst4CI ACNGT 4 cut(s) 511, 565, 778, 986
Bst6I CTCTTC 2 cut(s) 293, 421
BstAPI GCANNNNNTGC 1 cut(s) 794
BstAUI TGTACA 1 cut(s) 930
BstC8I GCNNGC 2 cut(s) 828, 1083
BstDEI CTNAG 3 cut(s) 477, 1005, 1061
BstEII GGTNACC 1 cut(s) 502
BstF5I GGATG 3 cut(s) 204, 232, 539
BstFNI CGCG 1 cut(s) 500
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 3 cut(s) 405, 652, 699
BstMBI GATC 3 cut(s) 402, 649, 696
BstMWI GCNNNNNNNGC 3 cut(s) 557, 794, 1115
BstNI CCWGG 1 cut(s) 743
BstNSI RCATGY 1 cut(s) 218
BstPAI GACNNNNGTC 1 cut(s) 509
BstPI GGTNACC 1 cut(s) 502
BstSCI CCNGG 1 cut(s) 741
BstSFI CTRYAG 1 cut(s) 972
BstSLI GKGCMC 1 cut(s) 1083
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 3 cut(s) 437, 644, 1002
BstXI CCANNNNNNTGG 1 cut(s) 58
BstZ17I GTATAC 1 cut(s) 13
BsuI GTATCC 2 cut(s) 381, 1049
BsuRI GGCC 1 cut(s) 84
BtgZI GCGATG 2 cut(s) 380, 675
BtsCI GGATG 3 cut(s) 204, 232, 539
BtsIMutI CAGTG 2 cut(s) 561, 933
Cac8I GCNNGC 2 cut(s) 828, 1083
CfoI GCGC 1 cut(s) 500
Cfr10I RCCGGY 2 cut(s) 809, 871
Cfr13I GGNCC 1 cut(s) 938
Csp6I GTAC 6 cut(s) 35, 313, 931, 948, 982, 987
CviAII CATG 9 cut(s) 58, 175, 209, 215, 406, 694, 942, 962, 1134
CviQI GTAC 6 cut(s) 35, 313, 931, 948, 982, 987
DdeI CTNAG 3 cut(s) 477, 1005, 1061
DpnI GATC 3 cut(s) 404, 651, 698
DpnII GATC 3 cut(s) 402, 649, 696
Eam1104I CTCTTC 2 cut(s) 293, 421
EarI CTCTTC 2 cut(s) 293, 421
Eco130I CCWWGG 4 cut(s) 16, 79, 520, 1086
Eco47I GGWCC 1 cut(s) 938
Eco57I CTGAAG 1 cut(s) 744
Eco88I CYCGRG 1 cut(s) 398
Eco91I GGTNACC 1 cut(s) 502
EcoO65I GGTNACC 1 cut(s) 502
EcoRI GAATTC 2 cut(s) 905, 1024
EcoRII CCWGG 1 cut(s) 741
EcoT14I CCWWGG 4 cut(s) 16, 79, 520, 1086
EcoT22I ATGCAT 1 cut(s) 847
ErhI CCWWGG 4 cut(s) 16, 79, 520, 1086
FaeI CATG 9 cut(s) 61, 178, 212, 218, 409, 697, 945, 965, 1137
FaqI GGGAC 1 cut(s) 1015
FatI CATG 9 cut(s) 57, 174, 208, 214, 405, 693, 941, 961, 1133
FauI CCCGC 1 cut(s) 1090
FbaI TGATCA 1 cut(s) 696
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 3 cut(s) 426, 658, 1016
FokI GGATG 3 cut(s) 211, 239, 546
Fsp4HI GCNGC 3 cut(s) 426, 658, 1016
FspBI CTAG 1 cut(s) 990
GlaI GCGC 1 cut(s) 499
GluI GCNGC 3 cut(s) 426, 658, 1016
GsuI CTGGAG 1 cut(s) 414
HaeIII GGCC 1 cut(s) 84
HapII CCGG 2 cut(s) 810, 872
HhaI GCGC 1 cut(s) 500
Hin1II CATG 9 cut(s) 61, 178, 212, 218, 409, 697, 945, 965, 1137
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HincII GTYRAC 1 cut(s) 152
HindII GTYRAC 1 cut(s) 152
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 1 cut(s) 370
HpaII CCGG 2 cut(s) 810, 872
HphI GGTGA 2 cut(s) 451, 514
Hpy166II GTNNAC 5 cut(s) 13, 152, 220, 931, 933
Hpy188I TCNGA 2 cut(s) 160, 613
Hpy188III TCNNGA 2 cut(s) 431, 1021
Hpy8I GTNNAC 5 cut(s) 13, 152, 220, 931, 933
HpyAV CCTTC 4 cut(s) 232, 563, 847, 1085
HpyCH4III ACNGT 4 cut(s) 511, 565, 778, 986
HpyCH4V TGCA 8 cut(s) 230, 338, 719, 765, 788, 830, 845, 974
HpyF10VI GCNNNNNNNGC 3 cut(s) 557, 794, 1115
HpyF3I CTNAG 3 cut(s) 477, 1005, 1061
Hsp92II CATG 9 cut(s) 61, 178, 212, 218, 409, 697, 945, 965, 1137
HspAI GCGC 1 cut(s) 498
Ksp22I TGATCA 1 cut(s) 696
Kzo9I GATC 3 cut(s) 402, 649, 696
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 3 cut(s) 131, 433, 787
Lsp1109I GCAGC 3 cut(s) 437, 644, 1002
LweI GCATC 4 cut(s) 217, 560, 797, 832
MaeI CTAG 1 cut(s) 990
MaeIII GTNAC 6 cut(s) 20, 248, 457, 502, 632, 967
MalI GATC 3 cut(s) 404, 651, 698
MboI GATC 3 cut(s) 402, 649, 696
MboII GAAGA 4 cut(s) 310, 408, 737, 851
MfeI CAATTG 1 cut(s) 492
MhlI GDGCHC 1 cut(s) 1083
MlyI GAGTC 1 cut(s) 379
MmeI TCCRAC 2 cut(s) 159, 435
MnlI CCTC 9 cut(s) 80, 101, 139, 177, 346, 378, 404, 659, 749
Mph1103I ATGCAT 1 cut(s) 847
MseI TTAA 3 cut(s) 291, 894, 956
MslI CAYNNNNRTG 3 cut(s) 213, 464, 1109
MspI CCGG 2 cut(s) 810, 872
MspR9I CCNGG 1 cut(s) 743
MunI CAATTG 1 cut(s) 492
MvaI CCWGG 1 cut(s) 743
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 3 cut(s) 557, 794, 1115
NdeII GATC 3 cut(s) 402, 649, 696
NlaIII CATG 9 cut(s) 61, 178, 212, 218, 409, 697, 945, 965, 1137
NlaIV GGNNCC 1 cut(s) 796
NmuCI GTSAC 4 cut(s) 457, 502, 632, 967
NsiI ATGCAT 1 cut(s) 847
NspI RCATGY 1 cut(s) 218
OliI CACNNNNGTG 1 cut(s) 464
PciI ACATGT 1 cut(s) 214
PciSI GCTCTTC 1 cut(s) 293
PkrI GCNGC 3 cut(s) 427, 659, 1017
PleI GAGTC 1 cut(s) 378
PpsI GAGTC 1 cut(s) 378
PscI ACATGT 1 cut(s) 214
PshAI GACNNNNGTC 1 cut(s) 509
PsiI TTATAA 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 741
PspEI GGTNACC 1 cut(s) 502
PspGI CCWGG 1 cut(s) 741
PspN4I GGNNCC 1 cut(s) 796
PspPI GGNCC 1 cut(s) 938
PstI CTGCAG 1 cut(s) 976
RsaI GTAC 6 cut(s) 36, 314, 932, 949, 983, 988
RsaNI GTAC 6 cut(s) 35, 313, 931, 948, 982, 987
RseI CAYNNNNRTG 3 cut(s) 213, 464, 1109
SapI GCTCTTC 1 cut(s) 293
SaqAI TTAA 3 cut(s) 291, 894, 956
SatI GCNGC 3 cut(s) 426, 658, 1016
Sau3AI GATC 3 cut(s) 402, 649, 696
Sau96I GGNCC 1 cut(s) 938
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 743
SduI GDGCHC 1 cut(s) 1083
SfaNI GCATC 4 cut(s) 217, 560, 797, 832
SfcI CTRYAG 1 cut(s) 972
SinI GGWCC 1 cut(s) 938
SmiMI CAYNNNNRTG 3 cut(s) 213, 464, 1109
SmlI CTYRAG 1 cut(s) 1019
SmoI CTYRAG 1 cut(s) 1019
SpeI ACTAGT 1 cut(s) 989
SsiI CCGC 3 cut(s) 500, 1083, 1131
SspMI CTAG 1 cut(s) 990
StyD4I CCNGG 1 cut(s) 741
StyI CCWWGG 4 cut(s) 16, 79, 520, 1086
TaaI ACNGT 4 cut(s) 511, 565, 778, 986
TaqI TCGA 4 cut(s) 205, 321, 445, 920
TatI WGTACW 3 cut(s) 34, 312, 930
Tru1I TTAA 3 cut(s) 291, 894, 956
Tru9I TTAA 3 cut(s) 291, 894, 956
TscAI CASTG 2 cut(s) 561, 940
TseFI GTSAC 4 cut(s) 457, 502, 632, 967
TseI GCWGC 3 cut(s) 425, 657, 1015
Tsp45I GTSAC 4 cut(s) 457, 502, 632, 967
TspDTI ATGAA 3 cut(s) 465, 852, 978
TspRI CASTG 2 cut(s) 561, 940
VpaK11BI GGWCC 1 cut(s) 938
XapI RAATTY 4 cut(s) 356, 759, 905, 1024
XceI RCATGY 1 cut(s) 218
XcmI CCANNNNNNNNNTGG 2 cut(s) 527, 749
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 1 cut(s) 990
ZrmI AGTACT 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.