pycom12g18940

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0019773
Physical Location & Seq
Forward (+)
20964061 .. 20965447
1387 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18940.1

Sequence Viewer

Length: 1134 bp
ATGCTGAATGTGATGTGTACCAACTTATCTAAACTCCAAGTGCTTACCAATACCATGGCTCTACTCATCACAAACCAAGGCCAATACCTCGCCTTTTTTCTGGTTCTCGTTTTGGCCATCTGCTCCCCTCAAGCTTTTTGTAGTCGCCAACATTCTGGGGACGACAAAATCATGTTGGAGAGGCACGAGGGCTGGATGGCTCGACATGGACGTGTTTACAAGGATTCACAAGAGAAGGAGAGGCGTTACAACACATTCAAGTCTAATGTGGAGTTTATAAAAGGTTTTAACAAGAAGGGCGAAAACCAGTACACACTCGGCATCAATAAATTTGCAGACCTAACTAATGAGGAGTTTCAGGCGATGCGTAAAGGCTACAAGAGGAGACACCTCCCGGAGATCATGTCCAACTCTGTGAAAGCAGCTCCAAATAACAATTTTCGATATGAAAATGTCACCGATGTGCCAAAATCTAAGGATTATTTTTCACTTGGCGCTGTGACCACTGTCAAAGACCAAAGACAATGTGGGTGTGGCTGGGCATTCTCGGCGGTGGCAGCCGTAGAAGGGCCTAACTTTCTCAAAACAGGAAACATAGTTTCGCTGTCAGAGCAAGAGCTTGTTGACTGCGATGTTGGTCTTGTGAACGATGGTTGCCATTATGGCGAAGTGGGTTATGCCTTTGAATACATGATTGAGCGAAACAGGAGCCTGGCAACATATGCTGATTACGGTTACAAGGGTACAGATGGAGGAAGTTGCAAGGCTGATGATTATAACAACAATGATGCTGCCATAACTGTAAAAGGATTCGAGCTTGTGCCTGAAAACAATGAGACGGCTCTGCTACAGGCTGTTGCAAACCAGCCAGTTTCTGTTGCCATTGATGATAATGGAGAATTTTTCCAGAATTATGCGGATGGTGTGTTCAGTGGGCCATGCGGCACAAACTTAACCCATGCTGTGATTGTCATTGGATATGGCGAAGAAGATGGGAAAAAGTATTGGCTGATCAAGAATTCTTGGGGAGAAGATTGGGGGCATAACGGGTACATGAAGTTGCAGAGAGACGTGCCTGCCAAGGAAGGACTCTGTGGCATAGCTGTGGCAGCTTATTATCCAACATCAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009791 GO:0009814 GO:0009817 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010150 GO:0010224 GO:0010282 GO:0010623 GO:0012501 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0034284 GO:0034285 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0046688 GO:0048366 GO:0048367 GO:0048437 GO:0048468 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0071704 GO:0080187 GO:0090567 GO:0090693 GO:0097501 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1990169
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

378

Amino Acids

42.07

Weight (kDa)

5.52

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 62 - 119 9.4e-20 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 155 - 375 5.6e-67 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019773)

Species Orthologous Gene IDs
malus_domestica MD04G1190100.v1.1 MD04G1190200.v1.1 MD12G1202900.v1.1 MD12G1203000.v1.1
prunus_persica Prupe.4G153800_v2.0.a1
pyrus_communis pycom04g16840 pycom12g18940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 278, 777
AciI CCGC 3 cut(s) 551, 917, 942
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 3 cut(s) 329, 899, 1018
AfaI GTAC 4 cut(s) 19, 311, 745, 1052
AfiI CCNNNNNNNGG 1 cut(s) 567
AflIII ACRYGT 1 cut(s) 211
AgsI TTSAA 2 cut(s) 259, 686
AjiI CACGTC 2 cut(s) 212, 1072
AjnI CCWGG 1 cut(s) 711
AleI CACNNNNGTG 1 cut(s) 461
AloI GAACNNNNNNTCC 2 cut(s) 911, 943
AluBI AGCT 6 cut(s) 134, 425, 619, 817, 1103, 1112
AluI AGCT 6 cut(s) 134, 425, 619, 817, 1103, 1112
Alw26I GTCTC 3 cut(s) 379, 830, 1062
AlwNI CAGNNNCTG 1 cut(s) 875
AoxI GGCC 4 cut(s) 79, 114, 569, 935
ApeKI GCWGC 4 cut(s) 422, 557, 791, 1109
ApoI RAATTY 3 cut(s) 329, 899, 1018
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 2 cut(s) 569, 935
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 448
BalI TGGCCA 1 cut(s) 116
BauI CACGAG 1 cut(s) 185
BbvI GCAGC 4 cut(s) 434, 569, 778, 1121
BccI CCATC 6 cut(s) 125, 190, 644, 743, 914, 986
BceAI ACGGC 2 cut(s) 545, 855
BciT130I CCWGG 1 cut(s) 713
BclI TGATCA 1 cut(s) 1011
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 3 cut(s) 379, 830, 1062
BfmI CTRYAG 1 cut(s) 848
BfoI RGCGCY 1 cut(s) 498
BglI GCCNNNNNGGC 1 cut(s) 663
BisI GCNGC 5 cut(s) 423, 558, 792, 943, 1110
BlsI GCNGC 5 cut(s) 424, 559, 793, 944, 1111
Bme1390I CCNGG 2 cut(s) 395, 713
BmgBI CACGTC 2 cut(s) 212, 1072
BmgT120I GGNCC 2 cut(s) 569, 935
BmiI GGNNCC 1 cut(s) 710
BmrFI CCNGG 2 cut(s) 395, 713
BmsI GCATC 3 cut(s) 330, 354, 778
BoxI GACNNNNGTC 1 cut(s) 506
BpuEI CTTGAG 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 395
BsaJI CCNNGG 3 cut(s) 54, 76, 1080
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 567
Bse1I ACTGG 2 cut(s) 307, 869
BseBI CCWGG 1 cut(s) 713
BseDI CCNNGG 3 cut(s) 54, 76, 1080
BseGI GGATG 2 cut(s) 201, 925
BseLI CCNNNNNNNGG 1 cut(s) 567
BseNI ACTGG 2 cut(s) 307, 869
BseRI GAGGAG 2 cut(s) 365, 397
BseXI GCAGC 4 cut(s) 434, 569, 778, 1121
BseYI CCCAGC 1 cut(s) 537
BshFI GGCC 4 cut(s) 81, 116, 571, 937
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 567
BsmAI GTCTC 3 cut(s) 379, 830, 1062
BsmBI CGTCTC 2 cut(s) 830, 1062
BsmFI GGGAC 1 cut(s) 173
BsmI GAATGC 1 cut(s) 542
BsnI GGCC 4 cut(s) 81, 116, 571, 937
Bsp143I GATC 2 cut(s) 399, 1011
Bsp19I CCATGG 1 cut(s) 54
BspACI CCGC 3 cut(s) 551, 917, 942
BspANI GGCC 4 cut(s) 81, 116, 571, 937
BspLI GGNNCC 1 cut(s) 710
BsrI ACTGG 2 cut(s) 307, 869
BssECI CCNNGG 3 cut(s) 54, 76, 1080
BssMI GATC 2 cut(s) 399, 1011
BssSI CACGAG 1 cut(s) 185
BssT1I CCWWGG 3 cut(s) 54, 76, 1080
Bst2BI CACGAG 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 713
Bst4CI ACNGT 3 cut(s) 508, 734, 802
BstAPI GCANNNNNTGC 1 cut(s) 722
BstC8I GCNNGC 1 cut(s) 1077
BstDEI CTNAG 1 cut(s) 474
BstDSI CCRYGG 1 cut(s) 54
BstF5I GGATG 2 cut(s) 201, 925
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 2 cut(s) 402, 1014
BstMAI GTCTC 3 cut(s) 379, 830, 1062
BstMBI GATC 2 cut(s) 399, 1011
BstMWI GCNNNNNNNGC 6 cut(s) 548, 557, 610, 663, 722, 1109
BstNI CCWGG 1 cut(s) 713
BstPAI GACNNNNGTC 1 cut(s) 506
BstSCI CCNGG 2 cut(s) 393, 711
BstSFI CTRYAG 1 cut(s) 848
BstV1I GCAGC 4 cut(s) 434, 569, 778, 1121
BstXI CCANNNNNNTGG 2 cut(s) 55, 155
BsuRI GGCC 4 cut(s) 81, 116, 571, 937
BtgI CCRYGG 1 cut(s) 54
BtgZI GCGATG 2 cut(s) 377, 645
BtrI CACGTC 2 cut(s) 212, 1072
BtsCI GGATG 2 cut(s) 201, 925
BtsIMutI CAGTG 2 cut(s) 504, 937
Cac8I GCNNGC 1 cut(s) 1077
CaiI CAGNNNCTG 1 cut(s) 875
CfoI GCGC 1 cut(s) 497
Cfr13I GGNCC 2 cut(s) 569, 935
Csp6I GTAC 4 cut(s) 18, 310, 744, 1051
CviAII CATG 8 cut(s) 55, 172, 206, 403, 691, 939, 959, 1054
CviQI GTAC 4 cut(s) 18, 310, 744, 1051
DdeI CTNAG 1 cut(s) 474
DpnI GATC 2 cut(s) 401, 1013
DpnII GATC 2 cut(s) 399, 1011
EaeI YGGCCR 1 cut(s) 114
Eco130I CCWWGG 3 cut(s) 54, 76, 1080
EcoO109I RGGNCCY 1 cut(s) 569
EcoRI GAATTC 1 cut(s) 1018
EcoRII CCWGG 1 cut(s) 711
EcoT14I CCWWGG 3 cut(s) 54, 76, 1080
ErhI CCWWGG 3 cut(s) 54, 76, 1080
Esp3I CGTCTC 2 cut(s) 830, 1062
FaeI CATG 8 cut(s) 58, 175, 209, 406, 694, 942, 962, 1057
FaqI GGGAC 1 cut(s) 173
FatI CATG 8 cut(s) 54, 171, 205, 402, 690, 938, 958, 1053
FauNDI CATATG 1 cut(s) 721
FbaI TGATCA 1 cut(s) 1011
Fnu4HI GCNGC 5 cut(s) 423, 558, 792, 943, 1110
FokI GGATG 2 cut(s) 208, 932
Fsp4HI GCNGC 5 cut(s) 423, 558, 792, 943, 1110
GlaI GCGC 1 cut(s) 496
GluI GCNGC 5 cut(s) 423, 558, 792, 943, 1110
GsaI CCCAGC 1 cut(s) 541
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 4 cut(s) 81, 116, 571, 937
HapII CCGG 1 cut(s) 395
HhaI GCGC 1 cut(s) 497
Hin1II CATG 8 cut(s) 58, 175, 209, 406, 694, 942, 962, 1057
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 1 cut(s) 132
HinfI GANTC 3 cut(s) 224, 810, 1089
HpaII CCGG 1 cut(s) 395
HphI GGTGA 1 cut(s) 448
Hpy166II GTNNAC 5 cut(s) 18, 217, 312, 625, 646
Hpy188I TCNGA 2 cut(s) 610, 1129
Hpy188III TCNNGA 2 cut(s) 907, 1015
Hpy8I GTNNAC 5 cut(s) 18, 217, 312, 625, 646
HpyAV CCTTC 4 cut(s) 229, 289, 560, 1079
HpyCH4III ACNGT 3 cut(s) 508, 734, 802
HpyCH4IV ACGT 2 cut(s) 211, 1071
HpyCH4V TGCA 4 cut(s) 335, 762, 860, 1063
HpyF10VI GCNNNNNNNGC 6 cut(s) 548, 557, 610, 663, 722, 1109
HpyF3I CTNAG 1 cut(s) 474
HpySE526I ACGT 2 cut(s) 211, 1071
Hsp92II CATG 8 cut(s) 58, 175, 209, 406, 694, 942, 962, 1057
HspAI GCGC 1 cut(s) 495
Ksp22I TGATCA 1 cut(s) 1011
Kzo9I GATC 2 cut(s) 399, 1011
LmnI GCTCC 3 cut(s) 128, 430, 708
Lsp1109I GCAGC 4 cut(s) 434, 569, 778, 1121
LweI GCATC 3 cut(s) 330, 354, 778
MaeII ACGT 2 cut(s) 211, 1071
MaeIII GTNAC 4 cut(s) 245, 454, 499, 734
MalI GATC 2 cut(s) 401, 1013
MboI GATC 2 cut(s) 399, 1011
MboII GAAGA 3 cut(s) 998, 1001, 1043
MlsI TGGCCA 1 cut(s) 116
MluCI AATT 5 cut(s) 329, 436, 899, 910, 1018
MluNI TGGCCA 1 cut(s) 116
MlyI GAGTC 1 cut(s) 1083
MmeI TCCRAC 2 cut(s) 156, 432
MnlI CCTC 9 cut(s) 98, 138, 174, 181, 234, 343, 375, 401, 746
Mox20I TGGCCA 1 cut(s) 116
MscI TGGCCA 1 cut(s) 116
MseI TTAA 2 cut(s) 288, 953
MslI CAYNNNNRTG 3 cut(s) 210, 461, 1103
Msp20I TGGCCA 1 cut(s) 116
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 2 cut(s) 395, 713
Mva1269I GAATGC 1 cut(s) 542
MvaI CCWGG 1 cut(s) 713
MwoI GCNNNNNNNGC 6 cut(s) 548, 557, 610, 663, 722, 1109
NciI CCSGG 1 cut(s) 395
NcoI CCATGG 1 cut(s) 54
NdeI CATATG 1 cut(s) 721
NdeII GATC 2 cut(s) 399, 1011
NlaIII CATG 8 cut(s) 58, 175, 209, 406, 694, 942, 962, 1057
NlaIV GGNNCC 1 cut(s) 710
NmeAIII GCCGAG 2 cut(s) 297, 527
NmuCI GTSAC 2 cut(s) 454, 499
OliI CACNNNNGTG 1 cut(s) 461
PcsI WCGNNNNNNNCGW 1 cut(s) 208
PctI GAATGC 1 cut(s) 542
PfeI GAWTC 2 cut(s) 224, 810
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 5 cut(s) 424, 559, 793, 944, 1111
PleI GAGTC 1 cut(s) 1083
PpsI GAGTC 1 cut(s) 1083
PshAI GACNNNNGTC 1 cut(s) 506
PsiI TTATAA 2 cut(s) 278, 777
Psp6I CCWGG 1 cut(s) 711
PspFI CCCAGC 1 cut(s) 537
PspGI CCWGG 1 cut(s) 711
PspN4I GGNNCC 1 cut(s) 710
PspPI GGNCC 2 cut(s) 569, 935
PstNI CAGNNNCTG 1 cut(s) 875
RsaI GTAC 4 cut(s) 19, 311, 745, 1052
RsaNI GTAC 4 cut(s) 18, 310, 744, 1051
RseI CAYNNNNRTG 3 cut(s) 210, 461, 1103
SaqAI TTAA 2 cut(s) 288, 953
SatI GCNGC 5 cut(s) 423, 558, 792, 943, 1110
Sau3AI GATC 2 cut(s) 399, 1011
Sau96I GGNCC 2 cut(s) 569, 935
SchI GAGTC 1 cut(s) 1083
ScrFI CCNGG 2 cut(s) 395, 713
SfaNI GCATC 3 cut(s) 330, 354, 778
SfcI CTRYAG 1 cut(s) 848
SmiMI CAYNNNNRTG 3 cut(s) 210, 461, 1103
SmlI CTYRAG 1 cut(s) 129
SmoI CTYRAG 1 cut(s) 129
Sse9I AATT 5 cut(s) 329, 436, 899, 910, 1018
SsiI CCGC 3 cut(s) 551, 917, 942
StyD4I CCNGG 2 cut(s) 393, 711
StyI CCWWGG 3 cut(s) 54, 76, 1080
TaaI ACNGT 3 cut(s) 508, 734, 802
TaiI ACGT 2 cut(s) 214, 1074
TaqI TCGA 3 cut(s) 202, 442, 813
TasI AATT 5 cut(s) 329, 436, 899, 910, 1018
TatI WGTACW 1 cut(s) 309
TauI GCSGC 1 cut(s) 945
TfiI GAWTC 2 cut(s) 224, 810
Tru1I TTAA 2 cut(s) 288, 953
Tru9I TTAA 2 cut(s) 288, 953
TscAI CASTG 2 cut(s) 511, 937
TseFI GTSAC 2 cut(s) 454, 499
TseI GCWGC 4 cut(s) 422, 557, 791, 1109
Tsp45I GTSAC 2 cut(s) 454, 499
TspDTI ATGAA 2 cut(s) 462, 1070
TspRI CASTG 2 cut(s) 511, 937
XapI RAATTY 3 cut(s) 329, 899, 1018
XcmI CCANNNNNNNNNTGG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.