MD12G1202900.v1.1

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0019773
Physical Location & Seq
Forward (+)
28316102 .. 28317979
1878 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1202900.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGGCTCTTCTCATCACAAACCAAGGCCAATCCCTCCGCTTGGCCTCATTTCTCATGCTCATTTTTGCCTTCTGCTCCTCGAAAGCATTTTGTAGTCGCGAACTTTATGGCGGCAACAAATCACAGTTGTCAGGCATGAGGAGTGGATGGCTCAACATGGACGCGCTTTATGATGCACAAAAGGAGATGCGCTACAACATATTCAAGAACAATGTGGAATTTATACAAGCTTTTAACAAAAACAAGGACGGAAAAAAGTACACACTCAGTGTCAATAAGTTTGCGGACAGAACTAACGAGGAAATTCGGCCAATGCGCAATGGCTACAAGAGAAAGGAACGTTCAGGGATCATGTCCAACTCTTTGATAGCATCTCCTTTTCGATATGGAAATGTCCCTAATGTGCCATCTTATATGGATTGGAGATGGTCTGGCGCTGTGACCTCGGTCAAAAACCAAGGATATGAATGTGAACAAGAGCTAGTTGACTGTGACACGAGTGGTTACGATCACGGTTGCAATGGCGGTAATTCAAATGTTGCGTATGACTACATGATCAAACGAAACAGGAGCCTTGCAACTGAAGATAATTACCCTTACCAGGCTAAAGATGGAGGAATTTGCAAAACTACCAGTGTTGTTCCTACATCTGGTGCCATAACCATAACCGGCTACGAAAATGCATTGTTGAAGGCTGTTGCGAACCAGCCGGTTTCTGTTGCCATTGATATCGACGCAGAGGAATTCAAGTATTATTCTGGTGGCGTGTACACTGGATCATGTGGTACAAACTTCCGCCACTGTGGTGCACCACAGACCTGTCCTGGGTTCGAGTTCTTGCTTGGGAGGGTCCTAGGGTGTGCGTGTGTAACTGAGTGTGGGTGTGGGGGTGCATCGGAGAGGAACGGAGAGAGTTGTCAAGAGGGAGAGATAAGGGGGAGATATATATATATATATATATATAATCAGAAAAAGAAGGAAAAGGGGAAGGGAAACGGGGACAAAACAGGGGATGGGCCTCTTGGGCTCATGGCTGTGTTCGTAACAATGTCAAACCACCGCCGGTCGATCTGCTTGGCCTTATTGCTTGTGCTAATTTTGGCCTTCCGGTCCGCCCAAGCATCCTCCAGTAGTGTGGTTTCCGTCGAGGGTGACAATAGCAAAAGCATGTTGGAGAGGTTCGAAGGGTGGATGGCGGAATATGGACGAGTTTATAAGGATGAACAAGAGAAGGAGATGCGCTTCAAGATATTCAAATCCACTGTGGAGTATATAGAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009791 GO:0009814 GO:0009817 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010150 GO:0010224 GO:0010282 GO:0010623 GO:0012501 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0034284 GO:0034285 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0046688 GO:0048366 GO:0048367 GO:0048437 GO:0048468 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0071704 GO:0080187 GO:0090567 GO:0090693 GO:0097501 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1990169
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

428

Amino Acids

47.54

Weight (kDa)

8.48

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 58 - 102 9.5e-11 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 158 - 267 7e-22 Papain family cysteine protease
Inhibitor_I29 PF08246 394 - 426 1.5e-07 Cathepsin propeptide inhibitor domain (I29)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019773)

Species Orthologous Gene IDs
malus_domestica MD04G1190100.v1.1 MD04G1190200.v1.1 MD12G1202900.v1.1 MD12G1203000.v1.1
prunus_persica Prupe.4G153800_v2.0.a1
pyrus_communis pycom04g16840 pycom12g18940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1215
Acc16I TGCGCA 1 cut(s) 317
AccB1I GGYRCC 1 cut(s) 653
AccII CGCG 2 cut(s) 99, 164
AciI CCGC 8 cut(s) 37, 111, 284, 525, 796, 1060, 1113, 1196
AclI AACGTT 1 cut(s) 340
AclWI GGATC 2 cut(s) 356, 784
AcoI YGGCCR 1 cut(s) 308
AcsI RAATTY 4 cut(s) 218, 303, 618, 743
AcuI CTGAAG 1 cut(s) 603
AdeI CACNNNGTG 1 cut(s) 269
AfaI GTAC 3 cut(s) 260, 770, 787
AfiI CCNNNNNNNGG 4 cut(s) 40, 601, 650, 825
AgsI TTSAA 6 cut(s) 205, 534, 691, 748, 1246, 1255
AjnI CCWGG 2 cut(s) 600, 823
AleI CACNNNNGTG 1 cut(s) 804
AluBI AGCT 3 cut(s) 230, 481, 1280
AluI AGCT 3 cut(s) 230, 481, 1280
Alw21I GWGCWC 1 cut(s) 811
Alw44I GTGCAC 1 cut(s) 807
AlwI GGATC 2 cut(s) 356, 784
AoxI GGCC 6 cut(s) 25, 42, 308, 1016, 1077, 1101
ApaLI GTGCAC 1 cut(s) 807
ApoI RAATTY 4 cut(s) 218, 303, 618, 743
AspA2I CCTAGG 1 cut(s) 853
AspLEI GCGC 5 cut(s) 166, 192, 318, 437, 1242
AspS9I GGNCC 3 cut(s) 850, 1016, 1110
AsuHPI GGTGA 1 cut(s) 1163
AsuII TTCGAA 1 cut(s) 1182
AvaII GGWCC 2 cut(s) 850, 1110
AvrII CCTAGG 1 cut(s) 853
BaeGI GKGCMC 1 cut(s) 811
BanI GGYRCC 1 cut(s) 653
BanII GRGCYC 1 cut(s) 1029
BauI CACGAG 1 cut(s) 496
Bbv12I GWGCWC 1 cut(s) 811
BccI CCATC 6 cut(s) 141, 415, 420, 605, 1007, 1186
BciT130I CCWGG 2 cut(s) 602, 825
BclI TGATCA 1 cut(s) 555
BfaI CTAG 2 cut(s) 482, 854
BfoI RGCGCY 1 cut(s) 438
BglI GCCNNNNNGGC 1 cut(s) 1024
BisI GCNGC 1 cut(s) 112
BlnI CCTAGG 1 cut(s) 853
BlsI GCNGC 1 cut(s) 113
Bme1390I CCNGG 2 cut(s) 602, 825
Bme18I GGWCC 2 cut(s) 850, 1110
BmgT120I GGNCC 3 cut(s) 850, 1016, 1110
BmiI GGNNCC 3 cut(s) 572, 655, 851
BmrFI CCNGG 2 cut(s) 602, 825
BmsI GCATC 6 cut(s) 163, 177, 380, 902, 1130, 1227
BoxI GACNNNNGTC 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 1111
Bpu14I TTCGAA 1 cut(s) 1182
BsaJI CCNNGG 5 cut(s) 22, 444, 457, 824, 853
BsaWI WCCGGW 1 cut(s) 1107
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 601, 650, 825
Bse118I RCCGGY 3 cut(s) 668, 709, 1062
Bse1I ACTGG 3 cut(s) 633, 778, 1128
Bse3DI GCAATG 2 cut(s) 325, 526
BseBI CCWGG 2 cut(s) 602, 825
BseDI CCNNGG 5 cut(s) 22, 444, 457, 824, 853
BseGI GGATG 5 cut(s) 152, 1018, 1121, 1197, 1225
BseLI CCNNNNNNNGG 4 cut(s) 40, 601, 650, 825
BseMI GCAATG 2 cut(s) 325, 526
BseMII CTCAG 2 cut(s) 280, 864
BseNI ACTGG 3 cut(s) 633, 778, 1128
BseRI GAGGAG 2 cut(s) 67, 154
BseSI GKGCMC 1 cut(s) 811
Bsh1236I CGCG 2 cut(s) 99, 164
Bsh1285I CGRYCG 1 cut(s) 1067
BshFI GGCC 6 cut(s) 27, 44, 310, 1018, 1079, 1103
BshNI GGYRCC 1 cut(s) 653
BsiEI CGRYCG 1 cut(s) 1067
BsiHKAI GWGCWC 1 cut(s) 811
BsiSI CCGG 4 cut(s) 669, 710, 1063, 1108
BslFI GGGAC 2 cut(s) 380, 1013
BslI CCNNNNNNNGG 4 cut(s) 40, 601, 650, 825
BsmFI GGGAC 2 cut(s) 380, 1013
BsnI GGCC 6 cut(s) 27, 44, 310, 1018, 1079, 1103
Bsp119I TTCGAA 1 cut(s) 1182
Bsp1286I GDGCHC 2 cut(s) 811, 1029
Bsp1407I TGTACA 1 cut(s) 768
Bsp143I GATC 5 cut(s) 348, 508, 555, 776, 1068
Bsp68I TCGCGA 1 cut(s) 99
BspACI CCGC 8 cut(s) 37, 111, 284, 525, 796, 1060, 1113, 1196
BspANI GGCC 6 cut(s) 27, 44, 310, 1018, 1079, 1103
BspCNI CTCAG 2 cut(s) 279, 865
BspFNI CGCG 2 cut(s) 99, 164
BspLI GGNNCC 3 cut(s) 572, 655, 851
BspPI GGATC 2 cut(s) 356, 784
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 1182
BspT107I GGYRCC 1 cut(s) 653
BsrDI GCAATG 2 cut(s) 325, 526
BsrFI RCCGGY 3 cut(s) 668, 709, 1062
BsrGI TGTACA 1 cut(s) 768
BsrI ACTGG 3 cut(s) 633, 778, 1128
BssAI RCCGGY 3 cut(s) 668, 709, 1062
BssECI CCNNGG 5 cut(s) 22, 444, 457, 824, 853
BssMI GATC 5 cut(s) 348, 508, 555, 776, 1068
BssSI CACGAG 1 cut(s) 496
BssT1I CCWWGG 3 cut(s) 22, 457, 853
Bst2BI CACGAG 1 cut(s) 496
Bst2UI CCWGG 2 cut(s) 602, 825
Bst4CI ACNGT 5 cut(s) 126, 491, 515, 803, 1264
Bst6I CTCTTC 1 cut(s) 12
BstAUI TGTACA 1 cut(s) 768
BstBI TTCGAA 1 cut(s) 1182
BstDEI CTNAG 2 cut(s) 266, 873
BstF5I GGATG 5 cut(s) 152, 1018, 1121, 1197, 1225
BstFNI CGCG 2 cut(s) 99, 164
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 5 cut(s) 166, 192, 318, 437, 1242
BstKTI GATC 5 cut(s) 351, 511, 558, 779, 1071
BstMBI GATC 5 cut(s) 348, 508, 555, 776, 1068
BstMCI CGRYCG 1 cut(s) 1067
BstMWI GCNNNNNNNGC 1 cut(s) 1024
BstNI CCWGG 2 cut(s) 602, 825
BstNSI RCATGY 1 cut(s) 1171
BstPAI GACNNNNGTC 1 cut(s) 446
BstSCI CCNGG 2 cut(s) 600, 823
BstSLI GKGCMC 1 cut(s) 811
BstUI CGCG 2 cut(s) 99, 164
BstXI CCANNNNNNTGG 1 cut(s) 1135
BsuRI GGCC 6 cut(s) 27, 44, 310, 1018, 1079, 1103
BtsCI GGATG 5 cut(s) 152, 1018, 1121, 1197, 1225
BtsIMutI CAGTG 5 cut(s) 274, 640, 771, 799, 1260
BtuMI TCGCGA 1 cut(s) 99
CfoI GCGC 5 cut(s) 166, 192, 318, 437, 1242
Cfr10I RCCGGY 3 cut(s) 668, 709, 1062
Cfr13I GGNCC 3 cut(s) 850, 1016, 1110
CpoI CGGWCCG 1 cut(s) 1110
CseI GACGC 2 cut(s) 170, 743
Csp6I GTAC 3 cut(s) 259, 769, 786
CspI CGGWCCG 1 cut(s) 1110
CviAII CATG 8 cut(s) 55, 136, 157, 352, 553, 780, 1030, 1168
CviQI GTAC 3 cut(s) 259, 769, 786
DdeI CTNAG 2 cut(s) 266, 873
DpnI GATC 5 cut(s) 350, 510, 557, 778, 1070
DpnII GATC 5 cut(s) 348, 508, 555, 776, 1068
DraIII CACNNNGTG 1 cut(s) 269
EaeI YGGCCR 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
EciI GGCGGA 3 cut(s) 785, 1102, 1211
Eco130I CCWWGG 3 cut(s) 22, 457, 853
Eco24I GRGCYC 1 cut(s) 1029
Eco32I GATATC 1 cut(s) 730
Eco47I GGWCC 2 cut(s) 850, 1110
Eco57I CTGAAG 1 cut(s) 603
EcoO109I RGGNCCY 1 cut(s) 850
EcoRI GAATTC 1 cut(s) 743
EcoRII CCWGG 2 cut(s) 600, 823
EcoRV GATATC 1 cut(s) 730
EcoT14I CCWWGG 3 cut(s) 22, 457, 853
EcoT22I ATGCAT 1 cut(s) 685
EcoT38I GRGCYC 1 cut(s) 1029
ErhI CCWWGG 3 cut(s) 22, 457, 853
FaeI CATG 8 cut(s) 58, 139, 160, 355, 556, 783, 1033, 1171
FaqI GGGAC 2 cut(s) 380, 1013
FatI CATG 8 cut(s) 54, 135, 156, 351, 552, 779, 1029, 1167
FbaI TGATCA 1 cut(s) 555
Fnu4HI GCNGC 1 cut(s) 112
FokI GGATG 5 cut(s) 159, 1025, 1108, 1204, 1232
FriOI GRGCYC 1 cut(s) 1029
Fsp4HI GCNGC 1 cut(s) 112
FspBI CTAG 2 cut(s) 482, 854
FspI TGCGCA 1 cut(s) 317
GlaI GCGC 5 cut(s) 165, 191, 317, 436, 1241
GluI GCNGC 1 cut(s) 112
GsuI CTGGAG 1 cut(s) 1111
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 6 cut(s) 27, 44, 310, 1018, 1079, 1103
HapII CCGG 4 cut(s) 669, 710, 1063, 1108
HgaI GACGC 2 cut(s) 170, 743
HhaI GCGC 5 cut(s) 166, 192, 318, 437, 1242
Hin1II CATG 8 cut(s) 58, 139, 160, 355, 556, 783, 1033, 1171
Hin6I GCGC 5 cut(s) 164, 190, 316, 435, 1240
HinP1I GCGC 5 cut(s) 164, 190, 316, 435, 1240
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HindIII AAGCTT 2 cut(s) 228, 1278
HpaII CCGG 4 cut(s) 669, 710, 1063, 1108
HphI GGTGA 1 cut(s) 1163
Hpy166II GTNNAC 6 cut(s) 261, 473, 487, 769, 771, 809
Hpy188I TCNGA 2 cut(s) 898, 969
Hpy188III TCNNGA 4 cut(s) 98, 205, 920, 1246
Hpy8I GTNNAC 6 cut(s) 261, 473, 487, 769, 771, 809
Hpy99I CGWCG 2 cut(s) 737, 1148
HpyAV CCTTC 7 cut(s) 79, 685, 970, 982, 1114, 1178, 1225
HpyCH4III ACNGT 5 cut(s) 126, 491, 515, 803, 1264
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 7 cut(s) 176, 519, 578, 624, 683, 809, 893
HpyF10VI GCNNNNNNNGC 1 cut(s) 1024
HpyF3I CTNAG 2 cut(s) 266, 873
HpySE526I ACGT 1 cut(s) 340
Hsp92II CATG 8 cut(s) 58, 139, 160, 355, 556, 783, 1033, 1171
HspAI GCGC 5 cut(s) 164, 190, 316, 435, 1240
Ksp22I TGATCA 1 cut(s) 555
Kzo9I GATC 5 cut(s) 348, 508, 555, 776, 1068
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 80, 570
LweI GCATC 6 cut(s) 163, 177, 380, 902, 1130, 1227
MaeI CTAG 2 cut(s) 482, 854
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 6 cut(s) 439, 491, 503, 868, 1042, 1151
MalI GATC 5 cut(s) 350, 510, 557, 778, 1070
MboI GATC 5 cut(s) 348, 508, 555, 776, 1068
MboII GAAGA 1 cut(s) 596
MhlI GDGCHC 2 cut(s) 811, 1029
MluCI AATT 7 cut(s) 218, 303, 529, 589, 618, 743, 1095
MmeI TCCRAC 2 cut(s) 381, 1152
Mph1103I ATGCAT 1 cut(s) 685
MseI TTAA 2 cut(s) 234, 1282
MslI CAYNNNNRTG 2 cut(s) 804, 1034
MspI CCGG 4 cut(s) 669, 710, 1063, 1108
MspR9I CCNGG 2 cut(s) 602, 825
MvaI CCWGG 2 cut(s) 602, 825
MvnI CGCG 2 cut(s) 99, 164
MwoI GCNNNNNNNGC 1 cut(s) 1024
NdeII GATC 5 cut(s) 348, 508, 555, 776, 1068
NlaIII CATG 8 cut(s) 58, 139, 160, 355, 556, 783, 1033, 1171
NlaIV GGNNCC 3 cut(s) 572, 655, 851
NmuCI GTSAC 3 cut(s) 439, 491, 1151
NruI TCGCGA 1 cut(s) 99
NsbI TGCGCA 1 cut(s) 317
NsiI ATGCAT 1 cut(s) 685
NspI RCATGY 1 cut(s) 1171
NspV TTCGAA 1 cut(s) 1182
OliI CACNNNNGTG 1 cut(s) 804
PciSI GCTCTTC 1 cut(s) 12
PkrI GCNGC 1 cut(s) 113
PpuMI RGGWCCY 1 cut(s) 850
PshAI GACNNNNGTC 1 cut(s) 446
PsiI TTATAA 1 cut(s) 1215
Psp1406I AACGTT 1 cut(s) 340
Psp5II RGGWCCY 1 cut(s) 850
Psp6I CCWGG 2 cut(s) 600, 823
PspGI CCWGG 2 cut(s) 600, 823
PspN4I GGNNCC 3 cut(s) 572, 655, 851
PspPI GGNCC 3 cut(s) 850, 1016, 1110
PspPPI RGGWCCY 1 cut(s) 850
RruI TCGCGA 1 cut(s) 99
RsaI GTAC 3 cut(s) 260, 770, 787
RsaNI GTAC 3 cut(s) 259, 769, 786
RseI CAYNNNNRTG 2 cut(s) 804, 1034
Rsr2I CGGWCCG 1 cut(s) 1110
RsrII CGGWCCG 1 cut(s) 1110
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 234, 1282
SatI GCNGC 1 cut(s) 112
Sau3AI GATC 5 cut(s) 348, 508, 555, 776, 1068
Sau96I GGNCC 3 cut(s) 850, 1016, 1110
ScrFI CCNGG 2 cut(s) 602, 825
SduI GDGCHC 2 cut(s) 811, 1029
SetI ASST 7 cut(s) 232, 343, 446, 483, 821, 1181, 1282
SfaNI GCATC 6 cut(s) 163, 177, 380, 902, 1130, 1227
SfuI TTCGAA 1 cut(s) 1182
SinI GGWCC 2 cut(s) 850, 1110
SmiMI CAYNNNNRTG 2 cut(s) 804, 1034
Sse9I AATT 7 cut(s) 218, 303, 529, 589, 618, 743, 1095
SsiI CCGC 8 cut(s) 37, 111, 284, 525, 796, 1060, 1113, 1196
SspMI CTAG 2 cut(s) 482, 854
StyD4I CCNGG 2 cut(s) 600, 823
StyI CCWWGG 3 cut(s) 22, 457, 853
TaaI ACNGT 5 cut(s) 126, 491, 515, 803, 1264
TaiI ACGT 1 cut(s) 343
TaqI TCGA 7 cut(s) 80, 382, 732, 831, 1067, 1146, 1182
TaqII GACCGA 1 cut(s) 436
TasI AATT 7 cut(s) 218, 303, 529, 589, 618, 743, 1095
TatI WGTACW 2 cut(s) 258, 768
TauI GCSGC 1 cut(s) 114
Tru1I TTAA 2 cut(s) 234, 1282
Tru9I TTAA 2 cut(s) 234, 1282
TscAI CASTG 5 cut(s) 274, 640, 778, 806, 1267
TseFI GTSAC 3 cut(s) 439, 491, 1151
Tsp45I GTSAC 3 cut(s) 439, 491, 1151
TspDTI ATGAA 2 cut(s) 480, 1236
TspGWI ACGGA 3 cut(s) 264, 921, 1132
TspRI CASTG 5 cut(s) 274, 640, 778, 806, 1267
VneI GTGCAC 1 cut(s) 807
VpaK11BI GGWCC 2 cut(s) 850, 1110
XapI RAATTY 4 cut(s) 218, 303, 618, 743
XceI RCATGY 1 cut(s) 1171
XcmI CCANNNNNNNNNTGG 1 cut(s) 608
XmaJI CCTAGG 1 cut(s) 853
XspI CTAG 2 cut(s) 482, 854
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.