Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011060

Physical Location & Seq

Genomic Coordinates Reverse (-)

28126485 .. 28130582

Gene Structure 4098 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1192000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1908 bp

ATGGAACACAAGGAGGAAAAAGAAGGGGAAGAAGACGAATATGGGATTCTTCTATATTACAAGTACACCCAAATCCCAGACATCGACGACCTCTTTGCCTTCTACAACTCCAACTGCAACTCCCTCTCTCTCCTCGGCCGTGTCCGTCTCTCTCCTCTAGGTGTCAACGTCACCGTTGGCGGGAAGTTATCTGCGTTGAGGAAGCACATTGCCGCGGTTGAGTCGAATAGTTTGTTCCATGGAACTGACTTCAAGCTTGCGACTTGTGGCCGCCCGTTGAATGACAAGGTTGCTGAAGAATGTGGGTTCACTTCTCTCTCGGTTCGCATTGTCAAGGAATTGGTTACCCTTAGCTCTAATCCTCTGCTAAAATCACCAGACATTTCGGATGCTGGAAAGCATCTCTCCGCGGTTGAGTTTCATTCAGTTCTAGAAAGAGCTGGACAATTTGACAAAGAGAGTATAGATAACAACAGCGAACTTGTTCTGTTGGATGCAAGGAACTTATATGAGACCAGAATTGGGAAATTCAACACACCCAATGTGGCAACTTTGGATCCAGGCATTAGGCAGTACAGTGATCTGCCCTCGTGGATAGATGATAACTCTGATCAATTGCAAGGGAAATCTGTCCTCATGTATTGTACTGGAGGAATCAGATGTGAAATGGCATCGGCTTATATTAGGTCAAAAGGTGCTGGATTTGAGAACGTGTTTCAGTTATATGGTGGAATACAGCGTTACTTGGAACAATTTCCAGATGGTGGTTTTTTCAAGGGAAAGAACTTTGTTTTCGATCATCGGATTTCCGTTGGGAGTTCAGATGCAAATATTATGGGCACCTGCCTTCTCTGTGGCTTGTCGTTTGATGATTATTCTTCACGCCTCCGTTGCAGTTATTGTAGAATGCTTGTCTTAGTTTGTGAGAGTTGCCAGAGGAAGGGCTCTGTATACGTATGTGAGCTTTGCCAGAATCATCACAAGGGTGTTCGGTTGATTTCTTCCACCGAAAATGGAAGATTAAGTTCAACTCCACAACAAATTGAGACTGAAACTGTCTCTTCCGATGCTAAGCCCTCGCCTCAGCTGCCTTGGAGACAGGATGTTGACTCTCCACCAAGAAAACTAAGAATCTTATGCCTACATGGGTTTCGTCAGAATGCCTCGAGTTTTAAGGGAAGAACGGCATCATTAGCGAAGAAACTCAAAAACATTGCTGAGCTCGTGTTTGTGGATGCACCTCACGAATTGCCATTCATATACCAATCCTCAACCTTTGCTGAGTCACATCACAATTGTAATTCACCAACTTCATCACTACTACAACAAAGTCGTCCACCACCGCCCTTGGAGCATTGCAAGAAAAAATTTGCATGGTTTGTAGCACCCGATTTCGTAGAAAAGAATGAAGGGGGGGAATGGAAAATGGAAGAGAGTCCATTTGATCCTCTTCAGTACCAGCAGCAAACAGCGGGGTTTGATGAATCATTAGCATATATCAAAACAGTGTATGCTGAGGAGGGACCCTTTGACGGACTGTTGGGATTTTCTCAGGGAGCGGCCATGGCAGCTGCTTTAGTCTGTGCCCAACAAACACAAAGGAGATCATTAACCGCAGGCGGAATGGAGTTTAGGTTTGTGATTCTGTGCTCCGGGTTCGGGCTTACAAAACTAATGCAGTCCGAAGAGGAGCAAGAGAGAGTTCCGATGTTGAAATGCCCGTCGCTCCACATATTTGGCAGTGAGCATGGCAAAGACAGGCAGATTGCAAACCAAGCAAGCAGAGATCTTGCTTCTTTATTTGATGATGGTTGTTCTCTAATCATTGAACACGACTCGGGTCATATCATTCCCACTCGTCCTCCTTACATCGATCGGATTAAGGACTTTCTTCGCCGGTTTCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FSH1 Rhodanese_C

Protein Analysis

636

Amino Acids

70.95

Weight (kDa)

5.98

Isoelectric Point (pI)

47.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UPF0176_N	PF17773	14 - 113	1.2e-14	UPF0176 acylphosphatase like domain
Rhodanese_C	PF12368	259 - 324	4.5e-14	Rhodanase C-terminal
FSH1	PF03959	374 - 625	2.2e-46	Serine hydrolase (FSH1)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011060)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09280
fragaria_vesca	FvH4_6g07770 FvH4_6g07770 FvH4_6g07770
malus_domestica	MD04G1192000.v1.1
prunus_persica	Prupe.6G310600_v2.0.a1 Prupe.6G310600_v2.0.a1
pyrus_communis	pycom04g16950
rosa_chinensis	RchiOBHm_Chr3g0457491
rosa_laevigata	RLG00000025219
rosa_multiflora	Rmu_co8113448.1_g000001 Rmu_co8194280.1_g000001 Rmu_sc0007404.1_g000017
rosa_roxburghii	Rroxscaffold_6G00421830
rosa_rugosa	Rorug03G0020500
rosa_samantha	Rh3AG081200 Rh3BG083300 Rh3CG083500 Rh3DG084700
rosa_wichuraiana	Rw3G006960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	851
Acc36I	ACCTGC	1 cut(s)	851
AccB1I	GGYRCC	1 cut(s)	839
AccB7I	CCANNNNNTGG	1 cut(s)	764
AccBSI	CCGCTC	1 cut(s)	1559
AccI	GTMKAC	1 cut(s)	951
AccII	CGCG	2 cut(s)	215, 410
AclWI	GGATC	3 cut(s)	551, 564, 1439
AcoI	YGGCCR	3 cut(s)	136, 268, 1560
AcsI	RAATTY	2 cut(s)	527, 1367
AcuI	CTGAAG	2 cut(s)	315, 1436
AfaI	GTAC	4 cut(s)	65, 575, 646, 1457
AfiI	CCNNNNNNNGG	6 cut(s)	180, 522, 764, 940, 1532, 1659
AflIII	ACRYGT	1 cut(s)	711
AgsI	TTSAA	7 cut(s)	253, 280, 532, 775, 1029, 1714, 1829
AjnI	CCWGG	1 cut(s)	559
AleI	CACNNNNGTG	1 cut(s)	984
AluBI	AGCT	7 cut(s)	256, 354, 440, 964, 1087, 1222, 1571
AluI	AGCT	7 cut(s)	256, 354, 440, 964, 1087, 1222, 1571
Alw21I	GWGCWC	2 cut(s)	1224, 1652
Alw26I	GTCTC	5 cut(s)	152, 506, 1040, 1063, 1090
AlwI	GGATC	3 cut(s)	551, 564, 1439
Ama87I	CYCGRG	2 cut(s)	1165, 1837
AoxI	GGCC	3 cut(s)	136, 268, 1560
ApeKI	GCWGC	4 cut(s)	1087, 1462, 1568, 1571
ApoI	RAATTY	2 cut(s)	527, 1367
ArsI	GACNNNNNNTTYG	2 cut(s)	77, 109
Asp700I	GAANNNNTTC	2 cut(s)	483, 753
AspS9I	GGNCC	1 cut(s)	1523
AsuC2I	CCSGG	1 cut(s)	1654
AsuHPI	GGTGA	3 cut(s)	163, 366, 1296
AvaI	CYCGRG	2 cut(s)	1165, 1837
AvaII	GGWCC	1 cut(s)	1523
BaeGI	GKGCMC	2 cut(s)	842, 1588
BamHI	GGATCC	1 cut(s)	556
BanI	GGYRCC	1 cut(s)	839
BanII	GRGCYC	2 cut(s)	947, 1224
BauI	CACGAG	2 cut(s)	589, 1223
BbsI	GAAGAC	1 cut(s)	39
Bbv12I	GWGCWC	2 cut(s)	1224, 1652
BbvCI	CCTCAGC	2 cut(s)	1083, 1515
BbvI	GCAGC	4 cut(s)	1074, 1474, 1558, 1580
BccI	CCATC	2 cut(s)	755, 1802
BceAI	ACGGC	2 cut(s)	123, 1200
BcgI	CGANNNNNNTGC	2 cut(s)	77, 111
BciT130I	CCWGG	1 cut(s)	561
BclI	TGATCA	1 cut(s)	610
BcnI	CCSGG	1 cut(s)	1654
BcoDI	GTCTC	5 cut(s)	152, 506, 1040, 1063, 1090
BfaI	CTAG	2 cut(s)	158, 431
BfuAI	ACCTGC	1 cut(s)	851
BglII	AGATCT	1 cut(s)	1786
BisI	GCNGC	7 cut(s)	213, 271, 1088, 1463, 1560, 1569, 1572
BlpI	GCTNAGC	2 cut(s)	1071, 1218
BlsI	GCNGC	7 cut(s)	214, 272, 1089, 1464, 1561, 1570, 1573
Bme1390I	CCNGG	2 cut(s)	561, 1654
Bme18I	GGWCC	1 cut(s)	1523
BmeT110I	CYCGRG	2 cut(s)	1165, 1837
BmgT120I	GGNCC	1 cut(s)	1523
BmiI	GGNNCC	4 cut(s)	558, 841, 1524, 1525
BmrFI	CCNGG	2 cut(s)	561, 1654
BmsI	GCATC	8 cut(s)	379, 409, 484, 680, 814, 1057, 1196, 1225
BoxI	GACNNNNGTC	1 cut(s)	1839
BpiI	GAAGAC	1 cut(s)	39
BpmI	CTGGAG	1 cut(s)	669
Bpu10I	CCTNAGC	3 cut(s)	350, 1083, 1515
Bpu1102I	GCTNAGC	2 cut(s)	1071, 1218
BpuMI	CCSGG	1 cut(s)	1654
Bsa29I	ATCGAT	1 cut(s)	1872
BsaAI	YACGTR	1 cut(s)	955
BsaI	GGTCTC	1 cut(s)	506
BsaJI	CCNNGG	7 cut(s)	133, 213, 238, 408, 1091, 1347, 1563
BsaXI	ACNNNNNCTCC	6 cut(s)	104, 134, 1619, 1649, 1846, 1876
Bsc4I	CCNNNNNNNGG	6 cut(s)	180, 522, 764, 940, 1532, 1659
Bse118I	RCCGGY	1 cut(s)	1896
Bse1I	ACTGG	1 cut(s)	652
Bse3DI	GCAATG	3 cut(s)	207, 1212, 1354
BseBI	CCWGG	1 cut(s)	561
BseCI	ATCGAT	1 cut(s)	1872
BseDI	CCNNGG	7 cut(s)	133, 213, 238, 408, 1091, 1347, 1563
BseGI	GGATG	4 cut(s)	394, 499, 1108, 1240
BseLI	CCNNNNNNNGG	6 cut(s)	180, 522, 764, 940, 1532, 1659
BseMI	GCAATG	3 cut(s)	207, 1212, 1354
BseMII	CTCAG	5 cut(s)	1097, 1209, 1272, 1506, 1565
BseNI	ACTGG	1 cut(s)	652
BseRI	GAGGAG	4 cut(s)	122, 144, 1532, 1703
BseSI	GKGCMC	2 cut(s)	842, 1588
BseX3I	CGGCCG	1 cut(s)	136
BseXI	GCAGC	4 cut(s)	1074, 1474, 1558, 1580
Bsh1236I	CGCG	2 cut(s)	215, 410
Bsh1285I	CGRYCG	2 cut(s)	139, 1876
BshFI	GGCC	3 cut(s)	138, 270, 1562
BshNI	GGYRCC	1 cut(s)	839
BshVI	ATCGAT	1 cut(s)	1872
BsiEI	CGRYCG	2 cut(s)	139, 1876
BsiHKAI	GWGCWC	2 cut(s)	1224, 1652
BsiHKCI	CYCGRG	2 cut(s)	1165, 1837
BsiSI	CCGG	2 cut(s)	1653, 1897
BslFI	GGGAC	1 cut(s)	1536
BslI	CCNNNNNNNGG	6 cut(s)	180, 522, 764, 940, 1532, 1659
BsmAI	GTCTC	5 cut(s)	152, 506, 1040, 1063, 1090
BsmBI	CGTCTC	1 cut(s)	152
BsmFI	GGGAC	1 cut(s)	1536
BsmI	GAATGC	2 cut(s)	912, 1165
BsnI	GGCC	3 cut(s)	138, 270, 1562
Bso31I	GGTCTC	1 cut(s)	506
BsoBI	CYCGRG	2 cut(s)	1165, 1837
Bsp1286I	GDGCHC	5 cut(s)	842, 947, 1224, 1588, 1652
Bsp143I	GATC	8 cut(s)	556, 580, 610, 796, 1444, 1604, 1786, 1873
Bsp1720I	GCTNAGC	2 cut(s)	1071, 1218
Bsp19I	CCATGG	2 cut(s)	238, 1563
BspANI	GGCC	3 cut(s)	138, 270, 1562
BspCNI	CTCAG	5 cut(s)	1096, 1210, 1273, 1507, 1564
BspDI	ATCGAT	1 cut(s)	1872
BspFNI	CGCG	2 cut(s)	215, 410
BspLI	GGNNCC	4 cut(s)	558, 841, 1524, 1525
BspMI	ACCTGC	1 cut(s)	851
BspPI	GGATC	3 cut(s)	551, 564, 1439
BspT107I	GGYRCC	1 cut(s)	839
BspTNI	GGTCTC	1 cut(s)	506
BsrBI	CCGCTC	1 cut(s)	1559
BsrDI	GCAATG	3 cut(s)	207, 1212, 1354
BsrFI	RCCGGY	1 cut(s)	1896
BsrI	ACTGG	1 cut(s)	652
BssAI	RCCGGY	1 cut(s)	1896
BssECI	CCNNGG	7 cut(s)	133, 213, 238, 408, 1091, 1347, 1563
BssMI	GATC	8 cut(s)	556, 580, 610, 796, 1444, 1604, 1786, 1873
BssNAI	GTATAC	1 cut(s)	952
BssSI	CACGAG	2 cut(s)	589, 1223
BssT1I	CCWWGG	4 cut(s)	238, 1091, 1347, 1563
Bst1107I	GTATAC	1 cut(s)	952
Bst2BI	CACGAG	2 cut(s)	589, 1223
Bst2UI	CCWGG	1 cut(s)	561
Bst4CI	ACNGT	5 cut(s)	175, 578, 1057, 1507, 1539
Bst6I	CTCTTC	4 cut(s)	1066, 1425, 1455, 1680
BstBAI	YACGTR	1 cut(s)	955
BstC8I	GCNNGC	3 cut(s)	258, 1618, 1780
BstDEI	CTNAG	9 cut(s)	350, 916, 1071, 1083, 1127, 1218, 1281, 1515, 1551
BstDSI	CCRYGG	4 cut(s)	213, 238, 408, 1563
BstEII	GGTNACC	1 cut(s)	343
BstF5I	GGATG	4 cut(s)	394, 499, 1108, 1240
BstFNI	CGCG	2 cut(s)	215, 410
BstKTI	GATC	8 cut(s)	559, 583, 613, 799, 1447, 1607, 1789, 1876
BstMAI	GTCTC	5 cut(s)	152, 506, 1040, 1063, 1090
BstMBI	GATC	8 cut(s)	556, 580, 610, 796, 1444, 1604, 1786, 1873
BstMCI	CGRYCG	2 cut(s)	139, 1876
BstMWI	GCNNNNNNNGC	7 cut(s)	891, 1087, 1193, 1351, 1565, 1568, 1775
BstNI	CCWGG	1 cut(s)	561
BstPAI	GACNNNNGTC	1 cut(s)	1839
BstPI	GGTNACC	1 cut(s)	343
BstSCI	CCNGG	2 cut(s)	559, 1652
BstSLI	GKGCMC	2 cut(s)	842, 1588
BstSNI	TACGTA	1 cut(s)	955
BstUI	CGCG	2 cut(s)	215, 410
BstV1I	GCAGC	4 cut(s)	1074, 1474, 1558, 1580
BstV2I	GAAGAC	1 cut(s)	39
BstX2I	RGATCY	2 cut(s)	556, 1786
BstXI	CCANNNNNNTGG	1 cut(s)	1736
BstYI	RGATCY	2 cut(s)	556, 1786
BstZ17I	GTATAC	1 cut(s)	952
BstZI	CGGCCG	1 cut(s)	136
Bsu15I	ATCGAT	1 cut(s)	1872
BsuRI	GGCC	3 cut(s)	138, 270, 1562
BsuTUI	ATCGAT	1 cut(s)	1872
BtgI	CCRYGG	4 cut(s)	213, 238, 408, 1563
BtsCI	GGATG	4 cut(s)	394, 499, 1108, 1240
BtsI	GCAGTG	1 cut(s)	1747
BtsIMutI	CAGTG	3 cut(s)	583, 1512, 1747
BveI	ACCTGC	1 cut(s)	851
Cac8I	GCNNGC	3 cut(s)	258, 1618, 1780
Cfr10I	RCCGGY	1 cut(s)	1896
Cfr13I	GGNCC	1 cut(s)	1523
Cfr42I	CCGCGG	2 cut(s)	216, 411
ClaI	ATCGAT	1 cut(s)	1872
Csp6I	GTAC	4 cut(s)	64, 574, 645, 1456
CspCI	CAANNNNNGTGG	2 cut(s)	1329, 1364
CviAII	CATG	6 cut(s)	239, 637, 1145, 1374, 1564, 1748
CviQI	GTAC	4 cut(s)	64, 574, 645, 1456
DdeI	CTNAG	9 cut(s)	350, 916, 1071, 1083, 1127, 1218, 1281, 1515, 1551
DpnI	GATC	8 cut(s)	558, 582, 612, 798, 1446, 1606, 1788, 1875
DpnII	GATC	8 cut(s)	556, 580, 610, 796, 1444, 1604, 1786, 1873
EaeI	YGGCCR	3 cut(s)	136, 268, 1560
EagI	CGGCCG	1 cut(s)	136
Eam1104I	CTCTTC	4 cut(s)	1066, 1425, 1455, 1680
EarI	CTCTTC	4 cut(s)	1066, 1425, 1455, 1680
EciI	GGCGGA	1 cut(s)	1635
Ecl136II	GAGCTC	1 cut(s)	1222
EclXI	CGGCCG	1 cut(s)	136
Eco105I	TACGTA	1 cut(s)	955
Eco130I	CCWWGG	4 cut(s)	238, 1091, 1347, 1563
Eco24I	GRGCYC	2 cut(s)	947, 1224
Eco31I	GGTCTC	1 cut(s)	506
Eco47I	GGWCC	1 cut(s)	1523
Eco52I	CGGCCG	1 cut(s)	136
Eco53kI	GAGCTC	1 cut(s)	1222
Eco57I	CTGAAG	2 cut(s)	315, 1436
Eco88I	CYCGRG	2 cut(s)	1165, 1837
Eco91I	GGTNACC	1 cut(s)	343
EcoICRI	GAGCTC	1 cut(s)	1222
EcoO109I	RGGNCCY	1 cut(s)	1523
EcoO65I	GGTNACC	1 cut(s)	343
EcoRII	CCWGG	1 cut(s)	559
EcoT14I	CCWWGG	4 cut(s)	238, 1091, 1347, 1563
EcoT38I	GRGCYC	2 cut(s)	947, 1224
ErhI	CCWWGG	4 cut(s)	238, 1091, 1347, 1563
Esp3I	CGTCTC	1 cut(s)	152
FaeI	CATG	6 cut(s)	242, 640, 1148, 1377, 1567, 1751
FaqI	GGGAC	1 cut(s)	1536
FatI	CATG	6 cut(s)	238, 636, 1144, 1373, 1563, 1747
FauI	CCCGC	2 cut(s)	173, 1465
FbaI	TGATCA	1 cut(s)	610
FblI	GTMKAC	1 cut(s)	951
Fnu4HI	GCNGC	7 cut(s)	213, 271, 1088, 1463, 1560, 1569, 1572
FokI	GGATG	4 cut(s)	401, 506, 1115, 1247
FriOI	GRGCYC	2 cut(s)	947, 1224
Fsp4HI	GCNGC	7 cut(s)	213, 271, 1088, 1463, 1560, 1569, 1572
FspBI	CTAG	2 cut(s)	158, 431
GluI	GCNGC	7 cut(s)	213, 271, 1088, 1463, 1560, 1569, 1572
GsuI	CTGGAG	1 cut(s)	669
HaeIII	GGCC	3 cut(s)	138, 270, 1562
HapII	CCGG	2 cut(s)	1653, 1897
Hin1II	CATG	6 cut(s)	242, 640, 1148, 1377, 1567, 1751
HincII	GTYRAC	2 cut(s)	166, 1108
HindII	GTYRAC	2 cut(s)	166, 1108
HindIII	AAGCTT	1 cut(s)	254
HpaII	CCGG	2 cut(s)	1653, 1897
HphI	GGTGA	3 cut(s)	163, 366, 1296
Hpy166II	GTNNAC	6 cut(s)	66, 166, 309, 952, 1108, 1337
Hpy188III	TCNNGA	3 cut(s)	431, 758, 1244
Hpy8I	GTNNAC	6 cut(s)	66, 166, 309, 952, 1108, 1337
Hpy99I	CGWCG	2 cut(s)	89, 1726
HpyAV	CCTTC	5 cut(s)	17, 109, 857, 934, 1403
HpyCH4III	ACNGT	5 cut(s)	175, 578, 1057, 1507, 1539
HpyCH4IV	ACGT	3 cut(s)	168, 711, 954
HpyF10VI	GCNNNNNNNGC	7 cut(s)	891, 1087, 1193, 1351, 1565, 1568, 1775
HpyF3I	CTNAG	9 cut(s)	350, 916, 1071, 1083, 1127, 1218, 1281, 1515, 1551
HpySE526I	ACGT	3 cut(s)	168, 711, 954
Hsp92II	CATG	6 cut(s)	242, 640, 1148, 1377, 1567, 1751
KflI	GGGWCCC	1 cut(s)	1523
Ksp22I	TGATCA	1 cut(s)	610
KspI	CCGCGG	2 cut(s)	216, 411
Kzo9I	GATC	8 cut(s)	556, 580, 610, 796, 1444, 1604, 1786, 1873
LmnI	GCTCC	5 cut(s)	1351, 1556, 1655, 1690, 1731
Lsp1109I	GCAGC	4 cut(s)	1074, 1474, 1558, 1580
LweI	GCATC	8 cut(s)	379, 409, 484, 680, 814, 1057, 1196, 1225
MaeI	CTAG	2 cut(s)	158, 431
MaeII	ACGT	3 cut(s)	168, 711, 954
MaeIII	GTNAC	4 cut(s)	169, 343, 740, 1284
MalI	GATC	8 cut(s)	558, 582, 612, 798, 1446, 1606, 1788, 1875
MbiI	CCGCTC	1 cut(s)	1559
MboI	GATC	8 cut(s)	556, 580, 610, 796, 1444, 1604, 1786, 1873
MfeI	CAATTG	2 cut(s)	614, 1294
MflI	RGATCY	2 cut(s)	556, 1786
MhlI	GDGCHC	5 cut(s)	842, 947, 1224, 1588, 1652
MlyI	GAGTC	5 cut(s)	230, 1103, 1292, 1444, 1829
MmeI	TCCRAC	2 cut(s)	135, 471
MroXI	GAANNNNTTC	2 cut(s)	483, 753
MseI	TTAA	4 cut(s)	1022, 1173, 1610, 1881
MslI	CAYNNNNRTG	1 cut(s)	984
MspA1I	CMGCKG	5 cut(s)	215, 410, 1087, 1472, 1571
MspI	CCGG	2 cut(s)	1653, 1897
MspR9I	CCNGG	2 cut(s)	561, 1654
MunI	CAATTG	2 cut(s)	614, 1294
Mva1269I	GAATGC	2 cut(s)	912, 1165
MvaI	CCWGG	1 cut(s)	561
MvnI	CGCG	2 cut(s)	215, 410
MwoI	GCNNNNNNNGC	7 cut(s)	891, 1087, 1193, 1351, 1565, 1568, 1775
NciI	CCSGG	1 cut(s)	1654
NcoI	CCATGG	2 cut(s)	238, 1563
NdeII	GATC	8 cut(s)	556, 580, 610, 796, 1444, 1604, 1786, 1873
NlaIII	CATG	6 cut(s)	242, 640, 1148, 1377, 1567, 1751
NlaIV	GGNNCC	4 cut(s)	558, 841, 1524, 1525
NmeAIII	GCCGAG	1 cut(s)	114
NmuCI	GTSAC	2 cut(s)	169, 1284
OliI	CACNNNNGTG	1 cut(s)	984
PaeR7I	CTCGAG	1 cut(s)	1165
PaqCI	CACCTGC	1 cut(s)	851
PctI	GAATGC	2 cut(s)	912, 1165
PdmI	GAANNNNTTC	2 cut(s)	483, 753
PfeI	GAWTC	6 cut(s)	46, 654, 973, 1131, 1484, 1642
PflMI	CCANNNNNTGG	1 cut(s)	764
PkrI	GCNGC	7 cut(s)	214, 272, 1089, 1464, 1561, 1570, 1573
Ple19I	CGATCG	1 cut(s)	1876
PleI	GAGTC	5 cut(s)	229, 1103, 1291, 1443, 1829
PpsI	GAGTC	5 cut(s)	229, 1103, 1291, 1443, 1829
Ppu21I	YACGTR	1 cut(s)	955
PpuMI	RGGWCCY	1 cut(s)	1523
PshAI	GACNNNNGTC	1 cut(s)	1839
Psp124BI	GAGCTC	1 cut(s)	1224
Psp5II	RGGWCCY	1 cut(s)	1523
Psp6I	CCWGG	1 cut(s)	559
PspEI	GGTNACC	1 cut(s)	343
PspGI	CCWGG	1 cut(s)	559
PspN4I	GGNNCC	4 cut(s)	558, 841, 1524, 1525
PspPI	GGNCC	1 cut(s)	1523
PspPPI	RGGWCCY	1 cut(s)	1523
PspXI	VCTCGAGB	1 cut(s)	1165
PsuI	RGATCY	2 cut(s)	556, 1786
PvuI	CGATCG	1 cut(s)	1876
PvuII	CAGCTG	2 cut(s)	1087, 1571
RsaI	GTAC	4 cut(s)	65, 575, 646, 1457
RsaNI	GTAC	4 cut(s)	64, 574, 645, 1456
RseI	CAYNNNNRTG	1 cut(s)	984
SacI	GAGCTC	1 cut(s)	1224
SacII	CCGCGG	2 cut(s)	216, 411
SaqAI	TTAA	4 cut(s)	1022, 1173, 1610, 1881
SatI	GCNGC	7 cut(s)	213, 271, 1088, 1463, 1560, 1569, 1572
Sau3AI	GATC	8 cut(s)	556, 580, 610, 796, 1444, 1604, 1786, 1873
Sau96I	GGNCC	1 cut(s)	1523
SchI	GAGTC	5 cut(s)	230, 1103, 1292, 1444, 1829
ScrFI	CCNGG	2 cut(s)	561, 1654
SduI	GDGCHC	5 cut(s)	842, 947, 1224, 1588, 1652
SfaNI	GCATC	8 cut(s)	379, 409, 484, 680, 814, 1057, 1196, 1225
Sfr274I	CTCGAG	1 cut(s)	1165
Sfr303I	CCGCGG	2 cut(s)	216, 411
SgrBI	CCGCGG	2 cut(s)	216, 411
SinI	GGWCC	1 cut(s)	1523
SlaI	CTCGAG	1 cut(s)	1165
SmiMI	CAYNNNNRTG	1 cut(s)	984
SmlI	CTYRAG	1 cut(s)	1165
SmoI	CTYRAG	1 cut(s)	1165
SnaBI	TACGTA	1 cut(s)	955
SspI	AATATT	1 cut(s)	832
SspMI	CTAG	2 cut(s)	158, 431
SstI	GAGCTC	1 cut(s)	1224
StyD4I	CCNGG	2 cut(s)	559, 1652
StyI	CCWWGG	4 cut(s)	238, 1091, 1347, 1563
TaaI	ACNGT	5 cut(s)	175, 578, 1057, 1507, 1539
TaiI	ACGT	3 cut(s)	171, 714, 957
TaqI	TCGA	5 cut(s)	84, 224, 795, 1166, 1872
TatI	WGTACW	3 cut(s)	63, 573, 644
TauI	GCSGC	3 cut(s)	215, 273, 1562
TfiI	GAWTC	6 cut(s)	46, 654, 973, 1131, 1484, 1642
Tru1I	TTAA	4 cut(s)	1022, 1173, 1610, 1881
Tru9I	TTAA	4 cut(s)	1022, 1173, 1610, 1881
TscAI	CASTG	3 cut(s)	583, 1512, 1747
TseFI	GTSAC	2 cut(s)	169, 1284
TseI	GCWGC	4 cut(s)	1087, 1462, 1568, 1571
Tsp45I	GTSAC	2 cut(s)	169, 1284
TspDTI	ATGAA	5 cut(s)	410, 1246, 1302, 1422, 1497
TspGWI	ACGGA	4 cut(s)	134, 799, 878, 1548
TspRI	CASTG	3 cut(s)	583, 1512, 1747
Van91I	CCANNNNNTGG	1 cut(s)	764
VpaK11BI	GGWCC	1 cut(s)	1523
XapI	RAATTY	2 cut(s)	527, 1367
XbaI	TCTAGA	1 cut(s)	430
XhoI	CTCGAG	1 cut(s)	1165
XmiI	GTMKAC	1 cut(s)	951
XmnI	GAANNNNTTC	2 cut(s)	483, 753
XspI	CTAG	2 cut(s)	158, 431

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.