Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0011060

Physical Location & Seq

Genomic Coordinates Reverse (-)

43056496 .. 43060194

Gene Structure 3699 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025219

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1908 bp

ATGGATCCCAATATTAACAGCAAAGAAGAACAAGAAGATGACGACCAACATGGGGTTCTTCTATACTACAAGTACACCCAAATTCCAGACCTCGACGACCTCTTCCACTTCTACGACTCCAACTGCAACTCCCTCTCACTCCTCGGCCGCGTTCGTCTCTCTCCTCTAGGCGTCAACGTCACCGTTGGTGGGAAGTTACGTGCGTTGAACAAGCACATTGCTGCGGTTGAGTCGAATAGTTTGTTTGAAGGGACTGATTTCAAGCTTGCAGCTTGTGGCCGACCGTCGAATGATAAGGTTGCCGAGGAATGTGGGTTCACTTCTCTCTCAATCCGCATTGTCAAGGAATTGGTTACTCTTAGCTGTAATCCTCTGATAAAATCGCCTGAGATTTCACATGCTGGAAAGCATCTTTCCGCAGCCGAGTTTCATTCCGTTTTACAGAAAGCTGGGAAACTAGACAAAGAGAGCAAAGAACACAATAGAGAACTTGTTCTATTGGATGCAAGGAATCTATATGAAACGAGAATTGGGAAGTTCCACACACCAAATGTGGAAACTTTGGACCCGGGCATTAGGCAGTACAGTGATCTTCCCTCATGGATAGATGATAACTCTGATCAATTGCAAGGAAAATCAGTTCTTATGTATTGCACTGGAGGTATCAGGTGTGAAATGGCATCAGCCTATATTAGGTCAAAAGGTGCTGGTTTTGAGAACGTGTTTCAGTTATATGGTGGAATTCAGCGTTATTTGGAACAGTTTCCAGATGGTGGTTTTTTCAGAGGGAAGAACTTTGTTTTTGATCACCGGATTTCAGTTGGGAGTTCAGATGCAAATATTCTGGGTGCCTGTCTTCTCTGTCGCACCTCATATGATGATTATTCGTCACGCCTTCGTTGCACTTATTGTAGAATGCTCGTCTTAGTTTGTGATAGTTGCCAGAGGAAGGGCTCTGTATTTGTTTGTGAGCTTTGCCAGAATCATCACAAGGGTGTTGGGTTGACTTCTTCTTTTGAAAATGGCGGAATCAGCTCACTACTATCTGTTGAAACAGTCTCTTCCAGCACTAAGCTCTCATGTCAAGTGCCTTGCGGACAGGCTACTGGGACTCCAAGAAAATTGAGAATCTTGTGCTTACATGGGTTTCGTCAGAATGCCTCGAGTTTCAAAGGAAGAACTGCCTCATTAGCCAAGAAACTCAAAAGCATTGCTGAGCTCGTGTTTGTGGATGCACCTCATGAATTGGCATTCATATACCAACCATCCTCGGCCCTAAGAGAGTTGCCTCACAATTGTGCATCATCACCAACTTCACCACAGCAAGGTCCTCCTCTTCCACCACCTTCGGAGCACTGCAAGAAAAAATACGCATGGCTTGTAGCACCTAATTTCCATAAAAAGACTGAAAGTGGTAATTGGGAAACGGTAGATGGTCCATTCGATCCACTGCAGTACCAACACCAAACTGCAGGGTTCGAAGAATCATTAGCGTATATCAAAACCGTGTTTGCACAGGAGGGCCCATTTGACGGAGTGCTCGGATTTTCACAGGGAGCAGCAATGGCAGCCTCTGTAGCTTTTGCCCAACCAACACAAACTATACCATCAAAAACTGCGGAAATGCAGTTTAGGTTTGTGATTTTGTGCTCTGGCTTCGGCATCAAGTTACTAAAGCAAGAGTTCCATGAGGAGAGAATGATCAAGAAATGCCATTGCCCTTCCCTCCACATATTCGGCAAGGATCACGGCAAAGACCGACAGATTGCCACGCAAGAAAGCCGAGAACTCGCTTCCTTATTCGATGACGGTTGCTCCACCATCATCGAACACGACTCCGGCCACATCATCCCTGCTCGTCCTCCCTACATTGATCAGATCAACGACTTCCTCTCCCGCTTCCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FSH1 Rhodanese_C

Protein Analysis

636

Amino Acids

70.42

Weight (kDa)

6.76

Isoelectric Point (pI)

42.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UPF0176_N	PF17773	19 - 116	1.4e-14	UPF0176 acylphosphatase like domain
Rhodanese_C	PF12368	262 - 327	1.2e-13	Rhodanase C-terminal
FSH1	PF03959	373 - 627	1.2e-45	Serine hydrolase (FSH1)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011060)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09280
fragaria_vesca	FvH4_6g07770 FvH4_6g07770 FvH4_6g07770
malus_domestica	MD04G1192000.v1.1
prunus_persica	Prupe.6G310600_v2.0.a1 Prupe.6G310600_v2.0.a1
pyrus_communis	pycom04g16950
rosa_chinensis	RchiOBHm_Chr3g0457491
rosa_laevigata	RLG00000025219
rosa_multiflora	Rmu_co8113448.1_g000001 Rmu_co8194280.1_g000001 Rmu_sc0007404.1_g000017
rosa_roxburghii	Rroxscaffold_6G00421830
rosa_rugosa	Rorug03G0020500
rosa_samantha	Rh3AG081200 Rh3BG083300 Rh3CG083500 Rh3DG084700
rosa_wichuraiana	Rw3G006960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	848
AccB7I	CCANNNNNTGG	1 cut(s)	773
AccII	CGCG	1 cut(s)	150
AciI	CCGC	8 cut(s)	148, 224, 334, 417, 1026, 1095, 1619, 1897
AclWI	GGATC	3 cut(s)	12, 1439, 1752
AcoI	YGGCCR	3 cut(s)	145, 277, 1840
AcsI	RAATTY	2 cut(s)	81, 741
AcyI	GRCGYC	1 cut(s)	171
AfaI	GTAC	3 cut(s)	74, 584, 1457
AfiI	CCNNNNNNNGG	7 cut(s)	52, 189, 693, 773, 949, 1325, 1532
AflIII	ACRYGT	1 cut(s)	720
AgsI	TTSAA	6 cut(s)	208, 248, 262, 1019, 1052, 1171
AjuI	GAANNNNNNNTTGG	4 cut(s)	39, 71, 513, 545
AleI	CACNNNNGTG	2 cut(s)	993, 1296
AloI	GAACNNNNNNTCC	4 cut(s)	299, 331, 624, 656
AluBI	AGCT	9 cut(s)	265, 272, 363, 449, 973, 1035, 1075, 1219, 1580
AluI	AGCT	9 cut(s)	265, 272, 363, 449, 973, 1035, 1075, 1219, 1580
Alw21I	GWGCWC	4 cut(s)	1221, 1356, 1542, 1652
Alw26I	GTCTC	2 cut(s)	161, 1063
AlwI	GGATC	3 cut(s)	12, 1439, 1752
AlwNI	CAGNNNCTG	1 cut(s)	1574
Ama87I	CYCGRG	2 cut(s)	568, 1162
AoxI	GGCC	5 cut(s)	145, 277, 1272, 1522, 1840
ApaI	GGGCCC	1 cut(s)	1526
ApeKI	GCWGC	5 cut(s)	221, 269, 419, 1559, 1568
ApoI	RAATTY	2 cut(s)	81, 741
Asp700I	GAANNNNTTC	2 cut(s)	492, 762
AspS9I	GGNCC	6 cut(s)	565, 1273, 1328, 1436, 1522, 1523
AsuC2I	CCSGG	2 cut(s)	569, 570
AsuHPI	GGTGA	4 cut(s)	172, 800, 1299, 1308
AsuII	TTCGAA	1 cut(s)	1479
AvaI	CYCGRG	2 cut(s)	568, 1162
AvaII	GGWCC	3 cut(s)	565, 1328, 1436
BaeGI	GKGCMC	1 cut(s)	1526
BaeI	ACNNNNGTAYC	2 cut(s)	1439, 1472
BamHI	GGATCC	1 cut(s)	4
BanI	GGYRCC	1 cut(s)	848
BanII	GRGCYC	3 cut(s)	956, 1221, 1526
BauI	CACGAG	1 cut(s)	1220
BbsI	GAAGAC	1 cut(s)	848
Bbv12I	GWGCWC	4 cut(s)	1221, 1356, 1542, 1652
BbvI	GCAGC	5 cut(s)	208, 281, 431, 1571, 1580
BccI	CCATC	5 cut(s)	764, 1273, 1427, 1615, 1829
BceAI	ACGGC	1 cut(s)	1765
BclI	TGATCA	4 cut(s)	619, 805, 1701, 1873
BcnI	CCSGG	2 cut(s)	569, 570
BcoDI	GTCTC	2 cut(s)	161, 1063
BfaI	CTAG	2 cut(s)	167, 458
BfmI	CTRYAG	3 cut(s)	1451, 1470, 1575
BisI	GCNGC	6 cut(s)	148, 222, 270, 420, 1560, 1569
BlpI	GCTNAGC	1 cut(s)	1215
BlsI	GCNGC	6 cut(s)	149, 223, 271, 421, 1561, 1570
Bme1390I	CCNGG	2 cut(s)	569, 570
Bme18I	GGWCC	3 cut(s)	565, 1328, 1436
BmeT110I	CYCGRG	2 cut(s)	568, 1162
BmgT120I	GGNCC	6 cut(s)	565, 1273, 1328, 1436, 1522, 1523
BmiI	GGNNCC	4 cut(s)	6, 567, 850, 1524
BmrFI	CCNGG	2 cut(s)	569, 570
BmrI	ACTGGG	1 cut(s)	1116
BmsI	GCATC	7 cut(s)	418, 493, 689, 823, 1222, 1310, 1671
BmuI	ACTGGG	1 cut(s)	1116
BpiI	GAAGAC	1 cut(s)	848
BpmI	CTGGAG	1 cut(s)	678
Bpu1102I	GCTNAGC	1 cut(s)	1215
Bpu14I	TTCGAA	1 cut(s)	1479
BpuMI	CCSGG	2 cut(s)	569, 570
BsaAI	YACGTR	1 cut(s)	200
BsaHI	GRCGYC	1 cut(s)	171
BsaJI	CCNNGG	4 cut(s)	142, 303, 568, 1269
BsaWI	WCCGGW	1 cut(s)	810
BsaXI	ACNNNNNCTCC	8 cut(s)	113, 143, 1096, 1126, 1799, 1829, 1877, 1907
Bsc4I	CCNNNNNNNGG	7 cut(s)	52, 189, 693, 773, 949, 1325, 1532
Bse1I	ACTGG	2 cut(s)	661, 1111
Bse3DI	GCAATG	4 cut(s)	216, 1209, 1569, 1714
BseDI	CCNNGG	4 cut(s)	142, 303, 568, 1269
BseGI	GGATG	4 cut(s)	508, 1237, 1265, 1848
BseLI	CCNNNNNNNGG	7 cut(s)	52, 189, 693, 773, 949, 1325, 1532
BseMI	GCAATG	4 cut(s)	216, 1209, 1569, 1714
BseMII	CTCAG	2 cut(s)	378, 1206
BseNI	ACTGG	2 cut(s)	661, 1111
BseRI	GAGGAG	4 cut(s)	131, 153, 1323, 1706
BseSI	GKGCMC	1 cut(s)	1526
BseX3I	CGGCCG	1 cut(s)	145
BseXI	GCAGC	5 cut(s)	208, 281, 431, 1571, 1580
BseYI	CCCAGC	1 cut(s)	449
Bsh1236I	CGCG	1 cut(s)	150
Bsh1285I	CGRYCG	2 cut(s)	148, 284
BshFI	GGCC	5 cut(s)	147, 279, 1274, 1524, 1842
BshNI	GGYRCC	1 cut(s)	848
BsiEI	CGRYCG	2 cut(s)	148, 284
BsiHKAI	GWGCWC	4 cut(s)	1221, 1356, 1542, 1652
BsiHKCI	CYCGRG	2 cut(s)	568, 1162
BsiSI	CCGG	3 cut(s)	569, 811, 1839
BslFI	GGGAC	2 cut(s)	265, 1123
BslI	CCNNNNNNNGG	7 cut(s)	52, 189, 693, 773, 949, 1325, 1532
BsmAI	GTCTC	2 cut(s)	161, 1063
BsmBI	CGTCTC	1 cut(s)	161
BsmFI	GGGAC	2 cut(s)	265, 1123
BsmI	GAATGC	3 cut(s)	921, 1162, 1250
BsnI	GGCC	5 cut(s)	147, 279, 1274, 1524, 1842
BsoBI	CYCGRG	2 cut(s)	568, 1162
Bsp119I	TTCGAA	1 cut(s)	1479
Bsp120I	GGGCCC	1 cut(s)	1522
Bsp1286I	GDGCHC	6 cut(s)	956, 1221, 1356, 1526, 1542, 1652
Bsp143I	GATC	9 cut(s)	4, 589, 619, 805, 1444, 1701, 1744, 1873, 1878
Bsp1720I	GCTNAGC	1 cut(s)	1215
BspACI	CCGC	8 cut(s)	148, 224, 334, 417, 1026, 1095, 1619, 1897
BspANI	GGCC	5 cut(s)	147, 279, 1274, 1524, 1842
BspCNI	CTCAG	2 cut(s)	379, 1207
BspFNI	CGCG	1 cut(s)	150
BspHI	TCATGA	1 cut(s)	1240
BspLI	GGNNCC	4 cut(s)	6, 567, 850, 1524
BspMAI	CTGCAG	2 cut(s)	1455, 1474
BspPI	GGATC	3 cut(s)	12, 1439, 1752
BspT104I	TTCGAA	1 cut(s)	1479
BspT107I	GGYRCC	1 cut(s)	848
BsrDI	GCAATG	4 cut(s)	216, 1209, 1569, 1714
BsrI	ACTGG	2 cut(s)	661, 1111
BssECI	CCNNGG	4 cut(s)	142, 303, 568, 1269
BssMI	GATC	9 cut(s)	4, 589, 619, 805, 1444, 1701, 1744, 1873, 1878
BssNI	GRCGYC	1 cut(s)	171
BssSI	CACGAG	1 cut(s)	1220
Bst2BI	CACGAG	1 cut(s)	1220
Bst4CI	ACNGT	8 cut(s)	184, 285, 587, 762, 1057, 1429, 1507, 1811
Bst6I	CTCTTC	3 cut(s)	107, 1066, 1341
BstACI	GRCGYC	1 cut(s)	171
BstBAI	YACGTR	1 cut(s)	200
BstBI	TTCGAA	1 cut(s)	1479
BstC8I	GCNNGC	1 cut(s)	267
BstDEI	CTNAG	6 cut(s)	359, 387, 925, 1071, 1215, 1277
BstENI	CCTNNNNNAGG	1 cut(s)	691
BstF5I	GGATG	4 cut(s)	508, 1237, 1265, 1848
BstFNI	CGCG	1 cut(s)	150
BstKTI	GATC	9 cut(s)	7, 592, 622, 808, 1447, 1704, 1747, 1876, 1881
BstMAI	GTCTC	2 cut(s)	161, 1063
BstMBI	GATC	9 cut(s)	4, 589, 619, 805, 1444, 1701, 1744, 1873, 1878
BstMCI	CGRYCG	2 cut(s)	148, 284
BstMWI	GCNNNNNNNGC	6 cut(s)	900, 1032, 1190, 1565, 1568, 1577
BstNSI	RCATGY	1 cut(s)	401
BstSCI	CCNGG	2 cut(s)	567, 568
BstSFI	CTRYAG	3 cut(s)	1451, 1470, 1575
BstSLI	GKGCMC	1 cut(s)	1526
BstUI	CGCG	1 cut(s)	150
BstV1I	GCAGC	5 cut(s)	208, 281, 431, 1571, 1580
BstV2I	GAAGAC	1 cut(s)	848
BstX2I	RGATCY	1 cut(s)	4
BstYI	RGATCY	1 cut(s)	4
BstZI	CGGCCG	1 cut(s)	145
BsuRI	GGCC	5 cut(s)	147, 279, 1274, 1524, 1842
BtsCI	GGATG	4 cut(s)	508, 1237, 1265, 1848
BtsI	GCAGTG	2 cut(s)	1354, 1448
BtsIMutI	CAGTG	4 cut(s)	592, 654, 1354, 1448
Cac8I	GCNNGC	1 cut(s)	267
CaiI	CAGNNNCTG	1 cut(s)	1574
CciI	TCATGA	1 cut(s)	1240
Cfr13I	GGNCC	6 cut(s)	565, 1273, 1328, 1436, 1522, 1523
Cfr9I	CCCGGG	1 cut(s)	568
CseI	GACGC	1 cut(s)	160
Csp6I	GTAC	3 cut(s)	73, 583, 1456
CviAII	CATG	8 cut(s)	50, 398, 600, 1080, 1142, 1241, 1374, 1688
CviQI	GTAC	3 cut(s)	73, 583, 1456
DdeI	CTNAG	6 cut(s)	359, 387, 925, 1071, 1215, 1277
DpnI	GATC	9 cut(s)	6, 591, 621, 807, 1446, 1703, 1746, 1875, 1880
DpnII	GATC	9 cut(s)	4, 589, 619, 805, 1444, 1701, 1744, 1873, 1878
EaeI	YGGCCR	3 cut(s)	145, 277, 1840
EagI	CGGCCG	1 cut(s)	145
Eam1104I	CTCTTC	3 cut(s)	107, 1066, 1341
EarI	CTCTTC	3 cut(s)	107, 1066, 1341
EciI	GGCGGA	1 cut(s)	1041
Ecl136II	GAGCTC	1 cut(s)	1219
EclXI	CGGCCG	1 cut(s)	145
Eco24I	GRGCYC	3 cut(s)	956, 1221, 1526
Eco47I	GGWCC	3 cut(s)	565, 1328, 1436
Eco52I	CGGCCG	1 cut(s)	145
Eco53kI	GAGCTC	1 cut(s)	1219
Eco88I	CYCGRG	2 cut(s)	568, 1162
EcoICRI	GAGCTC	1 cut(s)	1219
EcoNI	CCTNNNNNAGG	1 cut(s)	691
EcoO109I	RGGNCCY	2 cut(s)	1328, 1522
EcoRI	GAATTC	1 cut(s)	741
EcoT38I	GRGCYC	3 cut(s)	956, 1221, 1526
Esp3I	CGTCTC	1 cut(s)	161
FaeI	CATG	8 cut(s)	53, 401, 603, 1083, 1145, 1244, 1377, 1691
FaqI	GGGAC	2 cut(s)	265, 1123
FatI	CATG	8 cut(s)	49, 397, 599, 1079, 1141, 1240, 1373, 1687
FauI	CCCGC	1 cut(s)	1904
FauNDI	CATATG	1 cut(s)	874
FbaI	TGATCA	4 cut(s)	619, 805, 1701, 1873
Fnu4HI	GCNGC	6 cut(s)	148, 222, 270, 420, 1560, 1569
FokI	GGATG	4 cut(s)	515, 1244, 1252, 1835
FriOI	GRGCYC	3 cut(s)	956, 1221, 1526
Fsp4HI	GCNGC	6 cut(s)	148, 222, 270, 420, 1560, 1569
FspBI	CTAG	2 cut(s)	167, 458
GluI	GCNGC	6 cut(s)	148, 222, 270, 420, 1560, 1569
GsaI	CCCAGC	1 cut(s)	453
GsuI	CTGGAG	1 cut(s)	678
HaeIII	GGCC	5 cut(s)	147, 279, 1274, 1524, 1842
HapII	CCGG	3 cut(s)	569, 811, 1839
HgaI	GACGC	1 cut(s)	160
Hin1I	GRCGYC	1 cut(s)	171
Hin1II	CATG	8 cut(s)	53, 401, 603, 1083, 1145, 1244, 1377, 1691
HincII	GTYRAC	2 cut(s)	175, 1005
HindII	GTYRAC	2 cut(s)	175, 1005
HindIII	AAGCTT	1 cut(s)	263
HinfI	GANTC	9 cut(s)	116, 230, 511, 982, 1029, 1111, 1128, 1484, 1835
HpaII	CCGG	3 cut(s)	569, 811, 1839
HphI	GGTGA	4 cut(s)	172, 800, 1299, 1308
Hpy166II	GTNNAC	4 cut(s)	75, 175, 318, 1005
Hpy188I	TCNGA	8 cut(s)	375, 619, 785, 832, 1155, 1351, 1544, 1878
Hpy188III	TCNNGA	4 cut(s)	86, 767, 1241, 1705
Hpy8I	GTNNAC	4 cut(s)	75, 175, 318, 1005
Hpy99I	CGWCG	2 cut(s)	98, 289
HpyAV	CCTTC	5 cut(s)	242, 905, 943, 1356, 1731
HpyCH4III	ACNGT	8 cut(s)	184, 285, 587, 762, 1057, 1429, 1507, 1811
HpyCH4IV	ACGT	3 cut(s)	177, 199, 720
HpyF10VI	GCNNNNNNNGC	6 cut(s)	900, 1032, 1190, 1565, 1568, 1577
HpyF3I	CTNAG	6 cut(s)	359, 387, 925, 1071, 1215, 1277
HpySE526I	ACGT	3 cut(s)	177, 199, 720
Hsp92I	GRCGYC	1 cut(s)	171
Hsp92II	CATG	8 cut(s)	53, 401, 603, 1083, 1145, 1244, 1377, 1691
Ksp22I	TGATCA	4 cut(s)	619, 805, 1701, 1873
Kzo9I	GATC	9 cut(s)	4, 589, 619, 805, 1444, 1701, 1744, 1873, 1878
LmnI	GCTCC	3 cut(s)	1351, 1556, 1820
Lsp1109I	GCAGC	5 cut(s)	208, 281, 431, 1571, 1580
LweI	GCATC	7 cut(s)	418, 493, 689, 823, 1222, 1310, 1671
MaeI	CTAG	2 cut(s)	167, 458
MaeII	ACGT	3 cut(s)	177, 199, 720
MaeIII	GTNAC	5 cut(s)	178, 195, 352, 888, 1668
MalI	GATC	9 cut(s)	6, 591, 621, 807, 1446, 1703, 1746, 1875, 1880
MboI	GATC	9 cut(s)	4, 589, 619, 805, 1444, 1701, 1744, 1873, 1878
MfeI	CAATTG	2 cut(s)	623, 1294
MflI	RGATCY	1 cut(s)	4
MhlI	GDGCHC	6 cut(s)	956, 1221, 1356, 1526, 1542, 1652
MlyI	GAGTC	4 cut(s)	110, 239, 1105, 1829
MmeI	TCCRAC	1 cut(s)	144
MroXI	GAANNNNTTC	2 cut(s)	492, 762
MseI	TTAA	1 cut(s)	15
MslI	CAYNNNNRTG	2 cut(s)	993, 1296
MspI	CCGG	3 cut(s)	569, 811, 1839
MspR9I	CCNGG	2 cut(s)	569, 570
MunI	CAATTG	2 cut(s)	623, 1294
Mva1269I	GAATGC	3 cut(s)	921, 1162, 1250
MvnI	CGCG	1 cut(s)	150
MwoI	GCNNNNNNNGC	6 cut(s)	900, 1032, 1190, 1565, 1568, 1577
NciI	CCSGG	2 cut(s)	569, 570
NdeI	CATATG	1 cut(s)	874
NdeII	GATC	9 cut(s)	4, 589, 619, 805, 1444, 1701, 1744, 1873, 1878
NlaIII	CATG	8 cut(s)	53, 401, 603, 1083, 1145, 1244, 1377, 1691
NlaIV	GGNNCC	4 cut(s)	6, 567, 850, 1524
NmeAIII	GCCGAG	5 cut(s)	123, 328, 448, 1250, 1808
NmuCI	GTSAC	2 cut(s)	178, 888
NspI	RCATGY	1 cut(s)	401
NspV	TTCGAA	1 cut(s)	1479
OliI	CACNNNNGTG	2 cut(s)	993, 1296
PaeR7I	CTCGAG	1 cut(s)	1162
PagI	TCATGA	1 cut(s)	1240
PctI	GAATGC	3 cut(s)	921, 1162, 1250
PdmI	GAANNNNTTC	2 cut(s)	492, 762
PfeI	GAWTC	5 cut(s)	511, 982, 1029, 1128, 1484
PflMI	CCANNNNNTGG	1 cut(s)	773
PkrI	GCNGC	6 cut(s)	149, 223, 271, 421, 1561, 1570
PleI	GAGTC	4 cut(s)	110, 238, 1105, 1829
PpsI	GAGTC	4 cut(s)	110, 238, 1105, 1829
Ppu21I	YACGTR	1 cut(s)	200
PpuMI	RGGWCCY	1 cut(s)	1328
Psp124BI	GAGCTC	1 cut(s)	1221
Psp5II	RGGWCCY	1 cut(s)	1328
PspFI	CCCAGC	1 cut(s)	449
PspN4I	GGNNCC	4 cut(s)	6, 567, 850, 1524
PspOMI	GGGCCC	1 cut(s)	1522
PspPI	GGNCC	6 cut(s)	565, 1273, 1328, 1436, 1522, 1523
PspPPI	RGGWCCY	1 cut(s)	1328
PspXI	VCTCGAGB	1 cut(s)	1162
PstI	CTGCAG	2 cut(s)	1455, 1474
PstNI	CAGNNNCTG	1 cut(s)	1574
PsuI	RGATCY	1 cut(s)	4
RsaI	GTAC	3 cut(s)	74, 584, 1457
RsaNI	GTAC	3 cut(s)	73, 583, 1456
RseI	CAYNNNNRTG	2 cut(s)	993, 1296
SacI	GAGCTC	1 cut(s)	1221
SaqAI	TTAA	1 cut(s)	15
SatI	GCNGC	6 cut(s)	148, 222, 270, 420, 1560, 1569
Sau3AI	GATC	9 cut(s)	4, 589, 619, 805, 1444, 1701, 1744, 1873, 1878
Sau96I	GGNCC	6 cut(s)	565, 1273, 1328, 1436, 1522, 1523
SchI	GAGTC	4 cut(s)	110, 239, 1105, 1829
ScrFI	CCNGG	2 cut(s)	569, 570
SduI	GDGCHC	6 cut(s)	956, 1221, 1356, 1526, 1542, 1652
SfaNI	GCATC	7 cut(s)	418, 493, 689, 823, 1222, 1310, 1671
SfcI	CTRYAG	3 cut(s)	1451, 1470, 1575
Sfr274I	CTCGAG	1 cut(s)	1162
SfuI	TTCGAA	1 cut(s)	1479
SinI	GGWCC	3 cut(s)	565, 1328, 1436
SlaI	CTCGAG	1 cut(s)	1162
SmaI	CCCGGG	1 cut(s)	570
SmiMI	CAYNNNNRTG	2 cut(s)	993, 1296
SmlI	CTYRAG	1 cut(s)	1162
SmoI	CTYRAG	1 cut(s)	1162
SsiI	CCGC	8 cut(s)	148, 224, 334, 417, 1026, 1095, 1619, 1897
SspI	AATATT	2 cut(s)	13, 841
SspMI	CTAG	2 cut(s)	167, 458
SstI	GAGCTC	1 cut(s)	1221
StyD4I	CCNGG	2 cut(s)	567, 568
TaaI	ACNGT	8 cut(s)	184, 285, 587, 762, 1057, 1429, 1507, 1811
TaiI	ACGT	3 cut(s)	180, 202, 723
TaqI	TCGA	8 cut(s)	93, 233, 287, 1163, 1443, 1479, 1803, 1827
TaqII	GACCGA	1 cut(s)	1773
TatI	WGTACW	2 cut(s)	72, 582
TauI	GCSGC	1 cut(s)	150
TfiI	GAWTC	5 cut(s)	511, 982, 1029, 1128, 1484
Tru1I	TTAA	1 cut(s)	15
Tru9I	TTAA	1 cut(s)	15
TscAI	CASTG	4 cut(s)	592, 661, 1361, 1455
TseFI	GTSAC	2 cut(s)	178, 888
TseI	GCWGC	5 cut(s)	221, 269, 419, 1559, 1568
Tsp45I	GTSAC	2 cut(s)	178, 888
TspDTI	ATGAA	4 cut(s)	419, 534, 1243, 1257
TspGWI	ACGGA	2 cut(s)	424, 1548
TspMI	CCCGGG	1 cut(s)	568
TspRI	CASTG	4 cut(s)	592, 661, 1361, 1455
Van91I	CCANNNNNTGG	1 cut(s)	773
VpaK11BI	GGWCC	3 cut(s)	565, 1328, 1436
XagI	CCTNNNNNAGG	1 cut(s)	691
XapI	RAATTY	2 cut(s)	81, 741
XceI	RCATGY	1 cut(s)	401
XhoI	CTCGAG	1 cut(s)	1162
XmaI	CCCGGG	1 cut(s)	568
XmnI	GAANNNNTTC	2 cut(s)	492, 762
XspI	CTAG	2 cut(s)	167, 458

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.