Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011572

Physical Location & Seq

Genomic Coordinates Reverse (-)

29727050 .. 29731040

Gene Structure 3991 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1213800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1605 bp

ATGACTCGCAGCACCCTCCCGAGGGCATTGGCAGCTTCGGATCTTCATATCATGGATGGAACTCGAAGAAATTCCACACTGATATGTGCGCCGGTAGTGGCGGATTCAGTTGATCAGATGCTGGTCCAAATGGGGAAGGCAAAGGAAGTTGGCGCTGACCTCGTTGAACTTCGGGTTGATTTTTTGAAGAACTTCAGTCCAAGACAAGACCTCAGTGTATTGATCAAACAAAGTCCTTTGCCTACCCTTGTCACTTACAGGCCAGCATGGGAAGGCGGTCAATACAAGGGTGACGACAAGCAGCGACAAGATGCACTGCGTGTAGCCATGGATTTGGGAGCGGACTTTATTGACGTCGAACTTAAGGTAGCCGACGAGTTCTACAATTCCATTCAAGGGAGGAAGCCAGAAAAGGTCAAAATCATTGTTTCTTCACACAACTATGAGAAAACTCCATCTGCCGAGGAAATTGGTGATCTTGTTGCAAGGATACAAGCTACCGGAGCTGACATAGTGAAGATCGCGACTACTGGCTTAGACATCACCGACTCTGCACGTGTATTTCAAGTATTAGCGCATTCTCAAGTCCCAATGATAGGGCTGGTTATGGGCGAGAAGGGTTTGATCTCACGCGTACTTAGTGCAAAATATGGTGCATTCCTCACTTTTGGTACAATAGAGGCGGGAGTAGTATCGGCCCCTGGCCAGCCCACCGTAAGAGATCTACTGGACTTATACAACTTCAGACTTATAGGGGCCGATACCAAAGTACATGGTGTGATCGGAAATCCTATCGGTCACAGCAAAATCCCTCATCTTTACAATGCAGCATTCAGATCTATCAATTTCAATGGAATTTATTTGCCTTTGTTGGTTGATAACGTTGCGAATTTTATTGACACCTATAACTCCCCCGACTTTGTTGGATACAGTTACACAATTCCTCACGACGACGCTGGGATTAAATGCTGTGATGAAATCGATCCAAATGCCAAGGAAATAGGAGCTATTAGTTGCATGATCAGGAACCCGGCTGATGGCAAATTAAAAGGCTACAACATGGACTATCTAGGAGCAATTGCAGCCATTGAGGAAGGACTTCAAGCATTAAACGGTTCAAGTAATGGATCCGGCTCCCCATTAGCTGGTAAACTATTTGTCGTCATTGGAGCTGGTGGTGCTGGAAAGGCACTTGCATATGGTGGAAAACAGAAAGGAGCAAGAGTGGTAGTTGCCAATCGCACATATGACAAAGCCAAGGCACTTGCAAACAAAGTTGGAGGAGAAGCTATCACTCTTGCTGAATTGGAAAATTTCCATCCAGAGGAGGGAATGGTTCTTGCAAACACCACACCGGTTGGAATGAAGCCGAGAATTGATCAAACACCCTTATCCAAGAACGCTTTGAAGAACTATTCTTTGGTGTTTGATGCCATTCATACACCTAAATGGACAAGACTCTTACAAGAAGCACAAGAGTCTGGAGCAGCCGTTGTTTCCGGGACGGAAATGTTCCTCAATCAAGCATTTGTACAGGTTGAAAAGTTTTCTGGTTCACCTGCACCAAAGCAACTGATCAGGGATGTGTTGGCAAGAAATGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004764 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009423 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016491 GO:0016614 GO:0016616 GO:0019632 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046417 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055114 GO:0061458 GO:0071704 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 map00400 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

DHquinase_I Shikimate_DH Shikimate_dh_N

Protein Analysis

535

Amino Acids

57.43

Weight (kDa)

6.53

Isoelectric Point (pI)

25.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHquinase_I	PF01487	29 - 246	2.7e-67	Type I 3-dehydroquinase
Shikimate_dh_N	PF08501	260 - 338	5.4e-17	Shikimate dehydrogenase substrate binding domain
Shikimate_DH	PF01488	381 - 448	2.1e-08	Shikimate / quinate 5-dehydrogenase
SDH_C	PF18317	501 - 530	2.5e-06	Shikimate 5'-dehydrogenase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011572)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g04670 FvH4_6g04670 FvH4_6g04670
malus_domestica	MD04G1213800.v1.1 MD12G1228800.v1.1
prunus_persica	Prupe.6G334000_v2.0.a1 Prupe.6G334000_v2.0.a1
pyrus_communis	pycom04g18930 pycom12g21340
rosa_chinensis	RchiOBHm_Chr3g0453141
rosa_laevigata	RLG00000025534
rosa_multiflora	Rmu_sc0007132.1_g000006 Rmu_sc0013481.1_g000002
rosa_roxburghii	Rroxscaffold_6G00430200
rosa_rugosa	Rorug02G0646800
rosa_samantha	Rh3AG049400 Rh3BG050600 Rh3CG050800 Rh3DG051400
rosa_wichuraiana	Rw3G003790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1567
AatII	GACGTC	1 cut(s)	357
Acc36I	ACCTGC	1 cut(s)	1567
AccB7I	CCANNNNNTGG	1 cut(s)	1145
AccBSI	CCGCTC	1 cut(s)	341
AccII	CGCG	2 cut(s)	524, 633
AciI	CCGC	4 cut(s)	101, 276, 341, 683
AclI	AACGTT	1 cut(s)	882
AclWI	GGATC	4 cut(s)	48, 977, 1122, 1135
AcoI	YGGCCR	1 cut(s)	703
AcsI	RAATTY	4 cut(s)	70, 855, 889, 1312
AcuI	CTGAAG	2 cut(s)	178, 727
AcvI	CACGTG	1 cut(s)	557
AcyI	GRCGYC	1 cut(s)	354
AdeI	CACNNNGTG	1 cut(s)	320
AfaI	GTAC	4 cut(s)	636, 673, 771, 1533
AfiI	CCNNNNNNNGG	8 cut(s)	21, 22, 396, 596, 1037, 1145, 1324, 1328
AflII	CTTAAG	1 cut(s)	362
AflIII	ACRYGT	2 cut(s)	556, 631
AgeI	ACCGGT	1 cut(s)	1354
AgsI	TTSAA	9 cut(s)	167, 187, 395, 566, 850, 1103, 1119, 1408, 1541
AjnI	CCWGG	1 cut(s)	700
AjuI	GAANNNNNNNTTGG	2 cut(s)	1403, 1435
AluBI	AGCT	7 cut(s)	35, 497, 506, 1007, 1145, 1172, 1289
AluI	AGCT	7 cut(s)	35, 497, 506, 1007, 1145, 1172, 1289
AlwI	GGATC	4 cut(s)	48, 977, 1122, 1135
AlwNI	CAGNNNCTG	1 cut(s)	121
Ama87I	CYCGRG	1 cut(s)	19
AoxI	GGCC	4 cut(s)	260, 696, 703, 756
ApeKI	GCWGC	6 cut(s)	9, 32, 301, 827, 1082, 1487
ApoI	RAATTY	4 cut(s)	70, 855, 889, 1312
AsiGI	ACCGGT	1 cut(s)	1354
Asp700I	GAANNNNTTC	3 cut(s)	70, 191, 1098
AspLEI	GCGC	3 cut(s)	91, 155, 577
AspS9I	GGNCC	3 cut(s)	124, 697, 756
AsuC2I	CCSGG	2 cut(s)	1031, 1501
AsuHPI	GGTGA	4 cut(s)	302, 485, 535, 1548
AvaI	CYCGRG	1 cut(s)	19
AvaII	GGWCC	1 cut(s)	124
BaeI	ACNNNNGTAYC	2 cut(s)	753, 786
BalI	TGGCCA	1 cut(s)	705
BamHI	GGATCC	1 cut(s)	1127
BbrPI	CACGTG	1 cut(s)	557
BbvI	GCAGC	6 cut(s)	21, 44, 313, 839, 1094, 1499
BccI	CCATC	4 cut(s)	50, 463, 1031, 1326
BceAI	ACGGC	1 cut(s)	1475
BcgI	CGANNNNNNTGC	2 cut(s)	971, 1005
BciT130I	CCWGG	1 cut(s)	702
BciVI	GTATCC	2 cut(s)	483, 920
BclI	TGATCA	5 cut(s)	112, 222, 1020, 1378, 1575
BcnI	CCSGG	2 cut(s)	1031, 1501
BfaI	CTAG	1 cut(s)	1070
BfoI	RGCGCY	1 cut(s)	156
BfrI	CTTAAG	1 cut(s)	362
BfuAI	ACCTGC	1 cut(s)	1567
BfuI	GTATCC	2 cut(s)	483, 920
BglII	AGATCT	2 cut(s)	721, 836
BisI	GCNGC	6 cut(s)	10, 33, 302, 828, 1083, 1488
BlsI	GCNGC	6 cut(s)	11, 34, 303, 829, 1084, 1489
Bme1390I	CCNGG	3 cut(s)	702, 1031, 1501
Bme18I	GGWCC	1 cut(s)	124
BmeT110I	CYCGRG	1 cut(s)	19
BmgT120I	GGNCC	3 cut(s)	124, 697, 756
BmiI	GGNNCC	5 cut(s)	699, 757, 1028, 1129, 1135
BmrFI	CCNGG	3 cut(s)	702, 1031, 1501
BmsI	GCATC	3 cut(s)	108, 301, 1420
BpmI	CTGGAG	1 cut(s)	1503
BpuEI	CTTGAG	1 cut(s)	567
BpuMI	CCSGG	2 cut(s)	1031, 1501
Bsa29I	ATCGAT	1 cut(s)	981
BsaAI	YACGTR	1 cut(s)	557
BsaHI	GRCGYC	1 cut(s)	354
BsaJI	CCNNGG	6 cut(s)	20, 327, 462, 700, 993, 1257
BsaWI	WCCGGW	2 cut(s)	500, 1354
BsaXI	ACNNNNNCTCC	4 cut(s)	1209, 1239, 1476, 1506
Bsc4I	CCNNNNNNNGG	8 cut(s)	21, 22, 396, 596, 1037, 1145, 1324, 1328
Bse118I	RCCGGY	2 cut(s)	91, 1354
Bse1I	ACTGG	2 cut(s)	535, 732
BseBI	CCWGG	1 cut(s)	702
BseCI	ATCGAT	1 cut(s)	981
BseDI	CCNNGG	6 cut(s)	20, 327, 462, 700, 993, 1257
BseGI	GGATG	3 cut(s)	61, 1318, 1588
BseLI	CCNNNNNNNGG	8 cut(s)	21, 22, 396, 596, 1037, 1145, 1324, 1328
BseMII	CTCAG	1 cut(s)	226
BseNI	ACTGG	2 cut(s)	535, 732
BseRI	GAGGAG	2 cut(s)	1296, 1340
BseXI	GCAGC	6 cut(s)	21, 44, 313, 839, 1094, 1499
BseYI	CCCAGC	1 cut(s)	956
BsgI	GTGCAG	2 cut(s)	537, 1545
Bsh1236I	CGCG	2 cut(s)	524, 633
BshFI	GGCC	4 cut(s)	262, 698, 705, 758
BshTI	ACCGGT	1 cut(s)	1354
BshVI	ATCGAT	1 cut(s)	981
BsiHKCI	CYCGRG	1 cut(s)	19
BsiSI	CCGG	6 cut(s)	92, 501, 1031, 1131, 1355, 1500
BslFI	GGGAC	2 cut(s)	572, 1516
BslI	CCNNNNNNNGG	8 cut(s)	21, 22, 396, 596, 1037, 1145, 1324, 1328
BsmFI	GGGAC	2 cut(s)	572, 1516
BsmI	GAATGC	3 cut(s)	577, 656, 830
BsnI	GGCC	4 cut(s)	262, 698, 705, 758
BsoBI	CYCGRG	1 cut(s)	19
Bsp1407I	TGTACA	1 cut(s)	1531
Bsp19I	CCATGG	1 cut(s)	327
Bsp68I	TCGCGA	1 cut(s)	524
BspACI	CCGC	4 cut(s)	101, 276, 341, 683
BspANI	GGCC	4 cut(s)	262, 698, 705, 758
BspCNI	CTCAG	1 cut(s)	225
BspDI	ATCGAT	1 cut(s)	981
BspFNI	CGCG	2 cut(s)	524, 633
BspLI	GGNNCC	5 cut(s)	699, 757, 1028, 1129, 1135
BspMI	ACCTGC	1 cut(s)	1567
BspPI	GGATC	4 cut(s)	48, 977, 1122, 1135
BspTI	CTTAAG	1 cut(s)	362
BsrBI	CCGCTC	1 cut(s)	341
BsrFI	RCCGGY	2 cut(s)	91, 1354
BsrGI	TGTACA	1 cut(s)	1531
BsrI	ACTGG	2 cut(s)	535, 732
BssAI	RCCGGY	2 cut(s)	91, 1354
BssECI	CCNNGG	6 cut(s)	20, 327, 462, 700, 993, 1257
BssNI	GRCGYC	1 cut(s)	354
BssT1I	CCWWGG	3 cut(s)	327, 993, 1257
Bst2UI	CCWGG	1 cut(s)	702
Bst4CI	ACNGT	3 cut(s)	715, 932, 1115
BstACI	GRCGYC	1 cut(s)	354
BstAFI	CTTAAG	1 cut(s)	362
BstAPI	GCANNNNNTGC	1 cut(s)	1598
BstAUI	TGTACA	1 cut(s)	1531
BstBAI	YACGTR	1 cut(s)	557
BstC8I	GCNNGC	2 cut(s)	264, 707
BstDEI	CTNAG	3 cut(s)	212, 535, 638
BstDSI	CCRYGG	1 cut(s)	327
BstF5I	GGATG	3 cut(s)	61, 1318, 1588
BstFNI	CGCG	2 cut(s)	524, 633
BstH2I	RGCGCY	1 cut(s)	156
BstHHI	GCGC	3 cut(s)	91, 155, 577
BstMWI	GCNNNNNNNGC	6 cut(s)	32, 503, 1082, 1178, 1187, 1598
BstNI	CCWGG	1 cut(s)	702
BstSCI	CCNGG	3 cut(s)	700, 1029, 1499
BstUI	CGCG	2 cut(s)	524, 633
BstV1I	GCAGC	6 cut(s)	21, 44, 313, 839, 1094, 1499
BstX2I	RGATCY	4 cut(s)	40, 721, 836, 1127
BstXI	CCANNNNNNTGG	1 cut(s)	334
BstYI	RGATCY	4 cut(s)	40, 721, 836, 1127
Bsu15I	ATCGAT	1 cut(s)	981
BsuI	GTATCC	2 cut(s)	483, 920
BsuRI	GGCC	4 cut(s)	262, 698, 705, 758
BsuTUI	ATCGAT	1 cut(s)	981
BtgI	CCRYGG	1 cut(s)	327
BtsCI	GGATG	3 cut(s)	61, 1318, 1588
BtsI	GCAGTG	1 cut(s)	314
BtsIMutI	CAGTG	3 cut(s)	77, 220, 314
BtuMI	TCGCGA	1 cut(s)	524
BveI	ACCTGC	1 cut(s)	1567
Cac8I	GCNNGC	2 cut(s)	264, 707
CaiI	CAGNNNCTG	1 cut(s)	121
CfoI	GCGC	3 cut(s)	91, 155, 577
Cfr10I	RCCGGY	2 cut(s)	91, 1354
Cfr13I	GGNCC	3 cut(s)	124, 697, 756
ClaI	ATCGAT	1 cut(s)	981
CseI	GACGC	1 cut(s)	962
Csp6I	GTAC	4 cut(s)	635, 672, 770, 1532
CspAI	ACCGGT	1 cut(s)	1354
CspCI	CAANNNNNGTGG	2 cut(s)	1339, 1374
CviAII	CATG	7 cut(s)	52, 267, 328, 773, 1018, 1060, 1602
CviQI	GTAC	4 cut(s)	635, 672, 770, 1532
DdeI	CTNAG	3 cut(s)	212, 535, 638
DraIII	CACNNNGTG	1 cut(s)	320
EaeI	YGGCCR	1 cut(s)	703
EciI	GGCGGA	1 cut(s)	116
Eco130I	CCWWGG	3 cut(s)	327, 993, 1257
Eco47I	GGWCC	1 cut(s)	124
Eco57I	CTGAAG	2 cut(s)	178, 727
Eco72I	CACGTG	1 cut(s)	557
Eco88I	CYCGRG	1 cut(s)	19
EcoRII	CCWGG	1 cut(s)	700
EcoT14I	CCWWGG	3 cut(s)	327, 993, 1257
EcoT22I	ATGCAT	1 cut(s)	1603
ErhI	CCWWGG	3 cut(s)	327, 993, 1257
FaeI	CATG	7 cut(s)	55, 270, 331, 776, 1021, 1063, 1605
FaqI	GGGAC	2 cut(s)	572, 1516
FatI	CATG	7 cut(s)	51, 266, 327, 772, 1017, 1059, 1601
FauI	CCCGC	1 cut(s)	676
FauNDI	CATATG	2 cut(s)	1198, 1246
FbaI	TGATCA	5 cut(s)	112, 222, 1020, 1378, 1575
Fnu4HI	GCNGC	6 cut(s)	10, 33, 302, 828, 1083, 1488
FokI	GGATG	3 cut(s)	68, 1305, 1595
Fsp4HI	GCNGC	6 cut(s)	10, 33, 302, 828, 1083, 1488
FspBI	CTAG	1 cut(s)	1070
GlaI	GCGC	3 cut(s)	90, 154, 576
GluI	GCNGC	6 cut(s)	10, 33, 302, 828, 1083, 1488
GsaI	CCCAGC	1 cut(s)	960
GsuI	CTGGAG	1 cut(s)	1503
HaeII	RGCGCY	1 cut(s)	156
HaeIII	GGCC	4 cut(s)	262, 698, 705, 758
HapII	CCGG	6 cut(s)	92, 501, 1031, 1131, 1355, 1500
HgaI	GACGC	1 cut(s)	962
HhaI	GCGC	3 cut(s)	91, 155, 577
Hin1I	GRCGYC	1 cut(s)	354
Hin1II	CATG	7 cut(s)	55, 270, 331, 776, 1021, 1063, 1605
Hin6I	GCGC	3 cut(s)	89, 153, 575
HinP1I	GCGC	3 cut(s)	89, 153, 575
HinfI	GANTC	5 cut(s)	4, 104, 548, 1458, 1478
HpaII	CCGG	6 cut(s)	92, 501, 1031, 1131, 1355, 1500
HphI	GGTGA	4 cut(s)	302, 485, 535, 1548
Hpy166II	GTNNAC	2 cut(s)	1151, 1556
Hpy188I	TCNGA	5 cut(s)	40, 117, 746, 785, 836
Hpy188III	TCNNGA	6 cut(s)	19, 523, 947, 1024, 1322, 1482
Hpy8I	GTNNAC	2 cut(s)	1151, 1556
Hpy99I	CGWCG	4 cut(s)	359, 377, 953, 956
HpyAV	CCTTC	4 cut(s)	130, 266, 610, 1088
HpyCH4III	ACNGT	3 cut(s)	715, 932, 1115
HpyCH4IV	ACGT	3 cut(s)	354, 556, 882
HpyF10VI	GCNNNNNNNGC	6 cut(s)	32, 503, 1082, 1178, 1187, 1598
HpyF3I	CTNAG	3 cut(s)	212, 535, 638
HpySE526I	ACGT	3 cut(s)	354, 556, 882
Hsp92I	GRCGYC	1 cut(s)	354
Hsp92II	CATG	7 cut(s)	55, 270, 331, 776, 1021, 1063, 1605
HspAI	GCGC	3 cut(s)	89, 153, 575
Ksp22I	TGATCA	5 cut(s)	112, 222, 1020, 1378, 1575
LmnI	GCTCC	8 cut(s)	338, 503, 1004, 1073, 1139, 1169, 1217, 1484
Lsp1109I	GCAGC	6 cut(s)	21, 44, 313, 839, 1094, 1499
LweI	GCATC	3 cut(s)	108, 301, 1420
MaeI	CTAG	1 cut(s)	1070
MaeII	ACGT	3 cut(s)	354, 556, 882
MaeIII	GTNAC	4 cut(s)	250, 290, 797, 932
MbiI	CCGCTC	1 cut(s)	341
MboII	GAAGA	6 cut(s)	35, 78, 199, 423, 529, 1420
MfeI	CAATTG	1 cut(s)	1077
MflI	RGATCY	4 cut(s)	40, 721, 836, 1127
MlsI	TGGCCA	1 cut(s)	705
MluI	ACGCGT	1 cut(s)	631
MluNI	TGGCCA	1 cut(s)	705
MlyI	GAGTC	3 cut(s)	542, 1452, 1487
MmeI	TCCRAC	3 cut(s)	904, 1258, 1339
Mox20I	TGGCCA	1 cut(s)	705
Mph1103I	ATGCAT	1 cut(s)	1603
MroXI	GAANNNNTTC	3 cut(s)	70, 191, 1098
MscI	TGGCCA	1 cut(s)	705
MseI	TTAA	4 cut(s)	363, 963, 1046, 1109
MslI	CAYNNNNRTG	3 cut(s)	82, 441, 1447
Msp20I	TGGCCA	1 cut(s)	705
MspCI	CTTAAG	1 cut(s)	362
MspI	CCGG	6 cut(s)	92, 501, 1031, 1131, 1355, 1500
MspR9I	CCNGG	3 cut(s)	702, 1031, 1501
MunI	CAATTG	1 cut(s)	1077
Mva1269I	GAATGC	3 cut(s)	577, 656, 830
MvaI	CCWGG	1 cut(s)	702
MvnI	CGCG	2 cut(s)	524, 633
MwoI	GCNNNNNNNGC	6 cut(s)	32, 503, 1082, 1178, 1187, 1598
NciI	CCSGG	2 cut(s)	1031, 1501
NcoI	CCATGG	1 cut(s)	327
NdeI	CATATG	2 cut(s)	1198, 1246
NlaIII	CATG	7 cut(s)	55, 270, 331, 776, 1021, 1063, 1605
NlaIV	GGNNCC	5 cut(s)	699, 757, 1028, 1129, 1135
NmeAIII	GCCGAG	2 cut(s)	487, 1395
NmuCI	GTSAC	3 cut(s)	250, 290, 797
NruI	TCGCGA	1 cut(s)	524
NsiI	ATGCAT	1 cut(s)	1603
PaqCI	CACCTGC	1 cut(s)	1567
PctI	GAATGC	3 cut(s)	577, 656, 830
PdmI	GAANNNNTTC	3 cut(s)	70, 191, 1098
PfeI	GAWTC	1 cut(s)	104
PflMI	CCANNNNNTGG	1 cut(s)	1145
PfoI	TCCNGGA	1 cut(s)	1499
PinAI	ACCGGT	1 cut(s)	1354
PkrI	GCNGC	6 cut(s)	11, 34, 303, 829, 1084, 1489
PleI	GAGTC	3 cut(s)	542, 1452, 1486
PmaCI	CACGTG	1 cut(s)	557
PmlI	CACGTG	1 cut(s)	557
PpsI	GAGTC	3 cut(s)	542, 1452, 1486
Ppu21I	YACGTR	1 cut(s)	557
Psp1406I	AACGTT	1 cut(s)	882
Psp6I	CCWGG	1 cut(s)	700
PspCI	CACGTG	1 cut(s)	557
PspFI	CCCAGC	1 cut(s)	956
PspGI	CCWGG	1 cut(s)	700
PspN4I	GGNNCC	5 cut(s)	699, 757, 1028, 1129, 1135
PspPI	GGNCC	3 cut(s)	124, 697, 756
PstNI	CAGNNNCTG	1 cut(s)	121
PsuI	RGATCY	4 cut(s)	40, 721, 836, 1127
RruI	TCGCGA	1 cut(s)	524
RsaI	GTAC	4 cut(s)	636, 673, 771, 1533
RsaNI	GTAC	4 cut(s)	635, 672, 770, 1532
RseI	CAYNNNNRTG	3 cut(s)	82, 441, 1447
SaqAI	TTAA	4 cut(s)	363, 963, 1046, 1109
SatI	GCNGC	6 cut(s)	10, 33, 302, 828, 1083, 1488
Sau96I	GGNCC	3 cut(s)	124, 697, 756
SchI	GAGTC	3 cut(s)	542, 1452, 1487
ScrFI	CCNGG	3 cut(s)	702, 1031, 1501
SfaNI	GCATC	3 cut(s)	108, 301, 1420
SinI	GGWCC	1 cut(s)	124
SmiMI	CAYNNNNRTG	3 cut(s)	82, 441, 1447
SmlI	CTYRAG	2 cut(s)	362, 582
SmoI	CTYRAG	2 cut(s)	362, 582
SsiI	CCGC	4 cut(s)	101, 276, 341, 683
SspMI	CTAG	1 cut(s)	1070
StyD4I	CCNGG	3 cut(s)	700, 1029, 1499
StyI	CCWWGG	3 cut(s)	327, 993, 1257
TaaI	ACNGT	3 cut(s)	715, 932, 1115
TaiI	ACGT	3 cut(s)	357, 559, 885
TaqI	TCGA	3 cut(s)	64, 357, 981
TaqII	GACCGA	1 cut(s)	785
TatI	WGTACW	2 cut(s)	769, 1531
TfiI	GAWTC	1 cut(s)	104
Tru1I	TTAA	4 cut(s)	363, 963, 1046, 1109
Tru9I	TTAA	4 cut(s)	363, 963, 1046, 1109
TscAI	CASTG	3 cut(s)	84, 220, 321
TseFI	GTSAC	3 cut(s)	250, 290, 797
TseI	GCWGC	6 cut(s)	9, 32, 301, 827, 1082, 1487
Tsp45I	GTSAC	3 cut(s)	250, 290, 797
TspDTI	ATGAA	4 cut(s)	35, 990, 1379, 1427
TspGWI	ACGGA	1 cut(s)	1520
TspRI	CASTG	3 cut(s)	84, 220, 321
Van91I	CCANNNNNTGG	1 cut(s)	1145
Vha464I	CTTAAG	1 cut(s)	362
VpaK11BI	GGWCC	1 cut(s)	124
XapI	RAATTY	4 cut(s)	70, 855, 889, 1312
XmnI	GAANNNNTTC	3 cut(s)	70, 191, 1098
XspI	CTAG	1 cut(s)	1070
ZraI	GACGTC	1 cut(s)	355
Zsp2I	ATGCAT	1 cut(s)	1603

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.