MD04G1223900.v1.1

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013796
Physical Location & Seq
Reverse (-)
30399951 .. 30402958
3008 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1223900.v1.1.491

Sequence Viewer

Length: 465 bp
ATGGTCAAGCACAGAATGGGAGAGGTCCTAACTGAAGAACAGATTGTTGAGTTTCAGGAAGCTTTCTGTCTTTTTGACAAGGATGGAGATGGCTGCATTACTATTGAAGAACTTGCCACTGCGGTCAAATTGTTGGATCCAAACCCTACGGAAGAAGAATTGCAGAACATGATAAGCGAAGTTGACGTTGATGGGAACGGAACCATAGAGTTCGGGGAGTTCTTGAATCTCATGGCAAGAAAAATGAAGGAAAACGACGCGGATGAAGAATTGAAAGAAGCTTTCAAAGTTTTTGACAAGGATCAGGATGGCTACATTTCACCTAATGAGTTGAGGCATGTGATGGTAAATCTGGGAGAAAGACTGACAGATGAAGAGGTAGAGCAGATGATCAGAGAAGCAGATTTGGATGGTGATGGCTTAGTGAATTACGAAGAATTTGTGAGAATGATGTTAGCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1

Protein Analysis

155

Amino Acids

17.69

Weight (kDa)

4.13

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 17 - 44 1.9e-08 EF hand domain
EF-hand_6 PF13405 17 - 44 2.8e-07 EF-hand domain
EF-hand_7 PF13499 18 - 78 3.6e-15 EF-hand domain pair
EF-hand_9 PF14658 21 - 80 1.4e-06 EF-hand domain
EF-hand_8 PF13833 30 - 80 2.8e-09 EF-hand domain pair
EF-hand_1 PF00036 53 - 80 1.5e-07 EF hand domain
EF-hand_7 PF13499 89 - 151 3.1e-20 EF-hand domain pair
AIF-1 PF21008 89 - 152 3e-06 Allograft inflammatory factor 1
EF-hand_1 PF00036 90 - 116 1.3e-09 EF hand domain
EF-hand_6 PF13405 90 - 119 2.2e-09 EF-hand domain
EF-hand_5 PF13202 91 - 115 2.6e-07 EF hand
EF-hand_8 PF13833 105 - 151 3.5e-10 EF-hand domain pair
EF-hand_1 PF00036 126 - 152 2.1e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013796)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03000
malus_domestica MD04G1223900.v1.1
prunus_persica Prupe.6G342600_v2.0.a1 Prupe.6G342600_v2.0.a1
pyrus_communis pycom04g19730
rosa_chinensis RchiOBHm_Chr3g0451781
rosa_laevigata RLG00000025650
rosa_multiflora Rmu_sc0006510.1_g000021
rosa_roxburghii Rroxscaffold_164G00436320 Rroxscaffold_6G00428970
rosa_rugosa Rorug02G0636000 Rorug02G0636100 Rorug02G0636200.1
rosa_samantha Rh3AG037700 Rh3CG037300 Rh3DG038100
rosa_wichuraiana Rw3G002810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 260
AciI CCGC 2 cut(s) 122, 260
AclWI GGATC 3 cut(s) 131, 144, 309
AcsI RAATTY 1 cut(s) 437
AcuI CTGAAG 1 cut(s) 54
AgsI TTSAA 4 cut(s) 107, 226, 274, 286
AluBI AGCT 3 cut(s) 62, 281, 458
AluI AGCT 3 cut(s) 62, 281, 458
AlwI GGATC 3 cut(s) 131, 144, 309
ApeKI GCWGC 1 cut(s) 93
ApoI RAATTY 1 cut(s) 437
AspS9I GGNCC 1 cut(s) 25
AsuHPI GGTGA 2 cut(s) 312, 425
AvaII GGWCC 1 cut(s) 25
BamHI GGATCC 1 cut(s) 136
BbvI GCAGC 1 cut(s) 80
BccI CCATC 7 cut(s) 77, 83, 185, 302, 337, 404, 410
BclI TGATCA 1 cut(s) 390
BisI GCNGC 1 cut(s) 94
BlsI GCNGC 1 cut(s) 95
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 2 cut(s) 138, 202
BseGI GGATG 4 cut(s) 88, 268, 313, 415
BseXI GCAGC 1 cut(s) 80
Bsh1236I CGCG 1 cut(s) 260
Bsp143I GATC 3 cut(s) 136, 301, 390
BspACI CCGC 2 cut(s) 122, 260
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 2 cut(s) 138, 202
BspPI GGATC 3 cut(s) 131, 144, 309
BssMI GATC 3 cut(s) 136, 301, 390
Bst6I CTCTTC 1 cut(s) 369
BstDEI CTNAG 1 cut(s) 421
BstF5I GGATG 4 cut(s) 88, 268, 313, 415
BstFNI CGCG 1 cut(s) 260
BstKTI GATC 3 cut(s) 139, 304, 393
BstMBI GATC 3 cut(s) 136, 301, 390
BstNSI RCATGY 1 cut(s) 341
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 1 cut(s) 80
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BtsCI GGATG 4 cut(s) 88, 268, 313, 415
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 25
CseI GACGC 1 cut(s) 266
CviAII CATG 3 cut(s) 169, 232, 338
CviJI RGCY 6 cut(s) 62, 93, 281, 312, 420, 458
CviKI_1 RGCY 6 cut(s) 62, 93, 281, 312, 420, 458
DdeI CTNAG 1 cut(s) 421
DpnI GATC 3 cut(s) 138, 303, 392
DpnII GATC 3 cut(s) 136, 301, 390
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 54
EcoO109I RGGNCCY 1 cut(s) 25
FaeI CATG 3 cut(s) 172, 235, 341
FaiI YATR 4 cut(s) 170, 206, 233, 339
FatI CATG 3 cut(s) 168, 231, 337
FbaI TGATCA 1 cut(s) 390
Fnu4HI GCNGC 1 cut(s) 94
FokI GGATG 4 cut(s) 95, 275, 320, 422
Fsp4HI GCNGC 1 cut(s) 94
GluI GCNGC 1 cut(s) 94
HgaI GACGC 1 cut(s) 266
Hin1II CATG 3 cut(s) 172, 235, 341
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HindIII AAGCTT 2 cut(s) 60, 279
HinfI GANTC 1 cut(s) 226
HphI GGTGA 2 cut(s) 312, 425
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 1 cut(s) 395
Hpy188III TCNNGA 3 cut(s) 56, 223, 305
Hpy8I GTNNAC 1 cut(s) 184
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 1 cut(s) 241
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 96, 163
HpyF3I CTNAG 1 cut(s) 421
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 3 cut(s) 172, 235, 341
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 3 cut(s) 136, 301, 390
LpnPI CCDG 3 cut(s) 41, 290, 338
Lsp1109I GCAGC 1 cut(s) 80
MaeII ACGT 1 cut(s) 186
MalI GATC 3 cut(s) 138, 303, 392
MboI GATC 3 cut(s) 136, 301, 390
MboII GAAGA 7 cut(s) 47, 119, 164, 167, 278, 386, 446
MflI RGATCY 1 cut(s) 136
MluCI AATT 5 cut(s) 128, 158, 269, 427, 437
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 3 cut(s) 16, 327, 370
MvnI CGCG 1 cut(s) 260
NdeII GATC 3 cut(s) 136, 301, 390
NlaIII CATG 3 cut(s) 172, 235, 341
NlaIV GGNNCC 2 cut(s) 138, 202
NspI RCATGY 1 cut(s) 341
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 1 cut(s) 95
PpuMI RGGWCCY 1 cut(s) 25
Psp5II RGGWCCY 1 cut(s) 25
PspN4I GGNNCC 2 cut(s) 138, 202
PspPI GGNCC 1 cut(s) 25
PspPPI RGGWCCY 1 cut(s) 25
PsuI RGATCY 1 cut(s) 136
SatI GCNGC 1 cut(s) 94
Sau3AI GATC 3 cut(s) 136, 301, 390
Sau96I GGNCC 1 cut(s) 25
SetI ASST 7 cut(s) 27, 64, 189, 283, 325, 381, 460
SinI GGWCC 1 cut(s) 25
Sse9I AATT 5 cut(s) 128, 158, 269, 427, 437
SsiI CCGC 2 cut(s) 122, 260
TaiI ACGT 1 cut(s) 189
TasI AATT 5 cut(s) 128, 158, 269, 427, 437
TfiI GAWTC 1 cut(s) 226
TscAI CASTG 1 cut(s) 124
TseI GCWGC 1 cut(s) 93
TspDTI ATGAA 3 cut(s) 260, 279, 387
TspGWI ACGGA 2 cut(s) 164, 213
TspRI CASTG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 25
XapI RAATTY 1 cut(s) 437
XceI RCATGY 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.