MD04G1225700.v1.1

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015230
Physical Location & Seq
Forward (+)
30553182 .. 30555026
1845 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1225700.v1.1.491

Sequence Viewer

Length: 381 bp
ATGCAGCAGGGTGGAGGATCTGAGACGGAGGTGACATGGGAGGACCAGCAGAACATCAACAAATTTGGCAGGTTGAATAACCGGTTTCACGAGCTCGAGGACGAGATCAAATTTGCCAAGGAATCAAAGGATAATCTGGAGGATGCTAGCAACGAGTTGATTCTCACTGATGAGGAGGTGGTCCGGTTCCAGATAGGTGAAGTCTTTGCCCACGTGCCGAAAGAAGAAGTGGAGAGCAGGATAGAAGAGATGCAAGAGGCGACGAGCAAAAGTTTAGAGAAACTTGAAGAAGAGAAGGCATCTATTCTTGCACAGATGGCTGAGTTGAAGAAAATTCTGTATGGAAAGTTCAAGGACTCCATCAATCTAGAGGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006457 GO:0008150 GO:0009987 GO:0016272 GO:0032991 GO:0044424 GO:0044444 GO:0044464 GO:0051087
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prefoldin_2

Protein Analysis

127

Amino Acids

14.59

Weight (kDa)

4.5

Isoelectric Point (pI)

55.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin_2 PF01920 16 - 119 4.4e-22 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08780
fragaria_vesca FvH4_6g02700
malus_domestica MD04G1225700.v1.1 MD12G1242900.v1.1
prunus_persica Prupe.6G344500_v2.0.a1
pyrus_communis pycom12g22190
rosa_chinensis RchiOBHm_Chr3g0450701
rosa_laevigata RLG00000025709
rosa_multiflora Rmu_sc0000078.1_g000033
rosa_roxburghii Rroxscaffold_6G00428360
rosa_rugosa Rorug02G0629800
rosa_samantha Rh3AG030600 Rh3BG031100 Rh3CG029900 Rh3DG030700
rosa_wichuraiana Rw3G002360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 60
AclWI GGATC 1 cut(s) 25
AcsI RAATTY 3 cut(s) 62, 110, 333
AcvI CACGTG 1 cut(s) 214
AgeI ACCGGT 1 cut(s) 81
AgsI TTSAA 4 cut(s) 76, 287, 328, 352
AluBI AGCT 1 cut(s) 94
AluI AGCT 1 cut(s) 94
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 1 cut(s) 25
Ama87I CYCGRG 1 cut(s) 95
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 3 cut(s) 62, 110, 333
AsiGI ACCGGT 1 cut(s) 81
AspS9I GGNCC 2 cut(s) 43, 181
AsuHPI GGTGA 2 cut(s) 43, 209
AsuNHI GCTAGC 1 cut(s) 146
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 2 cut(s) 43, 181
BanII GRGCYC 1 cut(s) 96
BauI CACGAG 1 cut(s) 89
BbrPI CACGTG 1 cut(s) 214
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 1 cut(s) 16
BccI CCATC 2 cut(s) 310, 368
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 2 cut(s) 147, 368
BfuAI ACCTGC 1 cut(s) 60
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme18I GGWCC 2 cut(s) 43, 181
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 2 cut(s) 43, 181
BmiI GGNNCC 1 cut(s) 188
BmsI GCATC 3 cut(s) 133, 240, 308
BmtI GCTAGC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 158
BsaAI YACGTR 1 cut(s) 214
BsaJI CCNNGG 1 cut(s) 117
BsaWI WCCGGW 2 cut(s) 81, 183
Bse118I RCCGGY 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 1 cut(s) 148
BseMII CTCAG 2 cut(s) 12, 312
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 1 cut(s) 16
BshTI ACCGGT 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 2 cut(s) 82, 184
BsmAI GTCTC 1 cut(s) 17
BsmBI CGTCTC 1 cut(s) 17
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 17, 105
BspCNI CTCAG 2 cut(s) 13, 313
BspLI GGNNCC 1 cut(s) 188
BspMI ACCTGC 1 cut(s) 60
BspOI GCTAGC 1 cut(s) 150
BspPI GGATC 1 cut(s) 25
BsrFI RCCGGY 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 2 cut(s) 17, 105
BssSI CACGAG 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 117
Bst2BI CACGAG 1 cut(s) 89
Bst6I CTCTTC 2 cut(s) 240, 285
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 148
BstDEI CTNAG 2 cut(s) 21, 321
BstF5I GGATG 1 cut(s) 148
BstKTI GATC 2 cut(s) 20, 108
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 2 cut(s) 17, 105
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstV1I GCAGC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BtsCI GGATG 1 cut(s) 148
BtsIMutI CAGTG 1 cut(s) 165
BveI ACCTGC 1 cut(s) 60
Cac8I GCNNGC 1 cut(s) 148
Cfr10I RCCGGY 1 cut(s) 81
Cfr13I GGNCC 2 cut(s) 43, 181
CspAI ACCGGT 1 cut(s) 81
CviAII CATG 1 cut(s) 36
CviJI RGCY 2 cut(s) 94, 320
CviKI_1 RGCY 2 cut(s) 94, 320
DdeI CTNAG 2 cut(s) 21, 321
DpnI GATC 2 cut(s) 19, 107
DpnII GATC 2 cut(s) 17, 105
Eam1104I CTCTTC 2 cut(s) 240, 285
EarI CTCTTC 2 cut(s) 240, 285
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 96
Eco47I GGWCC 2 cut(s) 43, 181
Eco53kI GAGCTC 1 cut(s) 94
Eco72I CACGTG 1 cut(s) 214
Eco88I CYCGRG 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 117
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 117
Esp3I CGTCTC 1 cut(s) 17
FaeI CATG 1 cut(s) 39
FaiI YATR 2 cut(s) 37, 342
FatI CATG 1 cut(s) 35
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 1 cut(s) 155
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 2 cut(s) 147, 368
GluI GCNGC 1 cut(s) 5
GsuI CTGGAG 1 cut(s) 158
HapII CCGG 2 cut(s) 82, 184
Hin1II CATG 1 cut(s) 39
HinfI GANTC 3 cut(s) 122, 160, 356
HpaII CCGG 2 cut(s) 82, 184
HphI GGTGA 2 cut(s) 43, 209
Hpy188I TCNGA 1 cut(s) 22
Hpy188III TCNNGA 4 cut(s) 89, 137, 190, 368
Hpy99I CGWCG 1 cut(s) 265
HpyAV CCTTC 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 3 cut(s) 4, 253, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
HpyF3I CTNAG 2 cut(s) 21, 321
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 1 cut(s) 39
Kzo9I GATC 2 cut(s) 17, 105
LpnPI CCDG 7 cut(s) 55, 59, 95, 122, 197, 203, 223
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 133, 240, 308
MaeI CTAG 2 cut(s) 147, 368
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 1 cut(s) 31
MalI GATC 2 cut(s) 19, 107
MboI GATC 2 cut(s) 17, 105
MboII GAAGA 5 cut(s) 236, 257, 299, 302, 340
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 3 cut(s) 62, 110, 333
MlyI GAGTC 1 cut(s) 350
MseI TTAA 1 cut(s) 379
MspI CCGG 2 cut(s) 82, 184
MwoI GCNNNNNNNGC 1 cut(s) 317
NdeII GATC 2 cut(s) 17, 105
NheI GCTAGC 1 cut(s) 146
NlaIII CATG 1 cut(s) 39
NlaIV GGNNCC 1 cut(s) 188
NmuCI GTSAC 1 cut(s) 31
PaeR7I CTCGAG 1 cut(s) 95
PfeI GAWTC 2 cut(s) 122, 160
PinAI ACCGGT 1 cut(s) 81
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 1 cut(s) 350
PmaCI CACGTG 1 cut(s) 214
PmlI CACGTG 1 cut(s) 214
PpsI GAGTC 1 cut(s) 350
Ppu21I YACGTR 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 96
PspCI CACGTG 1 cut(s) 214
PspN4I GGNNCC 1 cut(s) 188
PspPI GGNCC 2 cut(s) 43, 181
PspXI VCTCGAGB 1 cut(s) 95
PsrI GAACNNNNNNTAC 2 cut(s) 332, 364
PsuI RGATCY 1 cut(s) 17
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 379
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 2 cut(s) 17, 105
Sau96I GGNCC 2 cut(s) 43, 181
SchI GAGTC 1 cut(s) 350
SduI GDGCHC 1 cut(s) 96
SetI ASST 6 cut(s) 33, 74, 96, 180, 199, 216
SfaNI GCATC 3 cut(s) 133, 240, 308
Sfr274I CTCGAG 1 cut(s) 95
SinI GGWCC 2 cut(s) 43, 181
SlaI CTCGAG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 3 cut(s) 62, 110, 333
SspMI CTAG 2 cut(s) 147, 368
SstI GAGCTC 1 cut(s) 96
StyI CCWWGG 1 cut(s) 117
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 96
TasI AATT 3 cut(s) 62, 110, 333
TfiI GAWTC 2 cut(s) 122, 160
Tru1I TTAA 1 cut(s) 379
Tru9I TTAA 1 cut(s) 379
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 31
TspGWI ACGGA 1 cut(s) 41
TspRI CASTG 1 cut(s) 172
VpaK11BI GGWCC 2 cut(s) 43, 181
XapI RAATTY 3 cut(s) 62, 110, 333
XbaI TCTAGA 1 cut(s) 367
XhoI CTCGAG 1 cut(s) 95
XspI CTAG 2 cut(s) 147, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.