MD04G1227500.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007026
Physical Location & Seq
Forward (+)
30699576 .. 30704249
4674 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1227500.v1.1.491

Sequence Viewer

Length: 2535 bp
ATGGCTGCCTCGGGGACTTCATCTGCATTGACGCAGAAAGCCCCCCAGGATTGGTCTAAAGCTTTGGTACACGCTCTTTTTGAATGGCTGCTGATTTCTATGCTGTTTGTTTACGCCATATTCTCATATGTTATTACGAAGTTTGCTTATTACTGTGGATTGCAAGCGCCTTGTCTGCTGTGTTCAAGAATTGATGATGTTTTGGGAAAGGAAAAACTTGGTTATTATTGGGACCTTTTCTGTGGAAATCATAAGTCAGAGATTTCTTCGTTGGTTCTTTGCCGTACTCACAATAAGCTTGTAGATGTTCATGGAATGTGTGAAAGTTGCCTCTTCTCATTTGCAACATTTAATAGATCCAATGCCGAGACTTGCGGATTACTGGTGGGTAAGTTGGGGGATGATCCTAAATTTGATTTCGATGAGGACCCTTTACTCGTGGGTCATAATACTTGCTCTTCAAGCAGAACGTTGTGTTCTTGTTGCAATCAGCCATGGATTTCTCGGGGGCATTCTCAAACGTTGATTCAGACAAAAATATTTGGCGCTGATGCTGAACTCGATATGCCCCTATCTCGTTCTATTGAACATAATCAGAAAGAACCGAGGAAGGCACGAGATGAATCATATATGTCAGCAAGAACCACTCATATGAGAGAGAATGGGATTCACCCTATATCTCATATCCGATACACAGAACTCAAGGTTAACTCTGATACTGAATCGGAAGTTCATTGTTCTGACAATGAAGGTGCAAGTGCTTTGATTCATGAAAGAGACAATCCCAAGGAAGATAAATCAGCTCCGAGAGATCCAGCATCTGTTCCCAAGCCTTCAATTTTGGTCTCACAGACACAAGTTGATTCTAAGTCCCATGGTAGTACATCTGTAGCATCTACTGTTGCTATCAGCCATGGACTGGAGGAACTTAACTGGCAACAAGTTGGAAGCAAGGCTGATCTCCCTCCGTCAACTGCACCCATCGTTGACAATCACCCTCCATCATCAAATGCAATGAAACCCCCAATTGAAGTATCAAAACAAAGAATTGATATTACTGGAAATCGTGAAATTGATCAAACATCTGTGGCGGAATCTGGGGAATTGTACAAGGGAGAAGTCAAGCCACTTACAACATCTGAAACAGGTTTGGAAACAAACCTTGTTTCAAGCAACGAACAATGGATTGGGAAGGACTCTTCTAAAGTTACTGAAGATTTGAAGGTCTTGCTGTCACAGTTGTCTACCACTAGAGGGCTTGAGCAATCAACAAGCGCAACGAGTCCGAGATTGTCTGCAAATAGTGGAAGTGCAATGAGTCCGAGATTGTCTGCAAATAGTGGAAGCGCAATGAGTCCGAGATTGTCTGCAAATAGTGGAAGCGTAATGAGTCCAAGATTGTCTGCAAATAGTGGTGATTTGAAAGCTTCGGATACTTCTAGTTATTTTGGAATGCAGATACATAACAAGAGGATCTCACTTGAAAGAAATGAGTCTGGGCTGTCCGTGGATGGGAGCATTGTCAGTGAAATTGAGGGTGAGAGTATGGTTGATCGATTAAAGCGTCAGGTTGAGCATGATAAGAAAATTATGAGCGCTTTGTATAAGGAGTTGGAGGAAGAAAGAAATGCTTCTTCAATAGCTTCAGATCAAGCAATGGCCATGATTACAAGACTGCAAGAGGAGAAGGCAGCACTCCGTATGGAAGCACTCCAGCAACTAAGAATGATGGAAGAGCAAGCCGAGTATGATAATGAAGCTCTGCAAAAGACAGACGACCTTCTTGTCGAGAAGGAGAAGGAAGTACAAGATCTGGAAGCGGAGCTGGAATTTTATAGGAATAAGTATCCCAATGAATCGATGCTAGATAATCTGGCAGAGACAACCGGTGATGTTCATGCAAGGGATATTGGGGTTGTTCATTCCGAATCAAGCAGCATGGAACATAGTTCAAGTGTACATAAACATGTGGATAATGGAAAGCCTCATACACACAGCAAAGTTGAAGGAGCCGCTACAACTTTTGGTGACAAGGATCGTAGTAGTGTAAAAGCTACACTTTTGGATTTCGAAGATGAGAAGATACAAATCTTACAGTATTTAGAGAAGTTAGAGAAGTCACTTTCTTTGCTCTCTGCCAATGGGGTAAATTCAGATTCGTCCAAAGGTGATTGTTCAAAAAAGGGAGGTTCCCAAGAAAACCCTCTGTCCGTGCAACATGAAGAGTCTGTATCATTGTCGGGACATACTGAATCACTTAAAAGCCCTCAGTCAAATGACAAGGGACAGTGTGATATTCGTTGCAGTGGGCCAAACTCTGCAGATCTCTGTCAAGTAACAGATTTAGCTTCTCTCCGGATCCTGAGTTCCAATGTGATCAAGAGGTTGAAGGCACTCGAGGCCGACCGAGAGTTTCTTGAACATGCGATTAACTCACTTAAACACGGTGAGGAGGGACTGACGTTCATTCAAGATATAGCTTCTTACCTGGGAGAACTACGACAAATCGGGATAAGAGATCAAACATCAGCTTGA

Protein Analysis

845

Amino Acids

92.71

Weight (kDa)

5.32

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 519 - 609 3.1e-29 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1784
AccB7I CCANNNNNTGG 1 cut(s) 919
AccI GTMKAC 1 cut(s) 1244
AccIII TCCGGA 1 cut(s) 2355
AciI CCGC 4 cut(s) 375, 1091, 1820, 2013
AclI AACGTT 2 cut(s) 470, 521
AclWI GGATC 7 cut(s) 351, 398, 806, 1481, 2043, 2353, 2366
AcoI YGGCCR 1 cut(s) 1659
AcsI RAATTY 3 cut(s) 410, 1829, 2149
AcuI CTGAAG 2 cut(s) 1233, 1629
AfaI GTAC 6 cut(s) 69, 286, 883, 1109, 1806, 1959
AfeI AGCGCT 1 cut(s) 1597
AfiI CCNNNNNNNGG 4 cut(s) 51, 919, 1254, 1512
AflIII ACRYGT 1 cut(s) 1966
AgeI ACCGGT 1 cut(s) 1886
AjnI CCWGG 2 cut(s) 45, 2487
AjuI GAANNNNNNNTTGG 2 cut(s) 1170, 1202
AloI GAACNNNNNNTCC 2 cut(s) 2350, 2382
Alw26I GTCTC 4 cut(s) 362, 771, 850, 1874
AlwI GGATC 7 cut(s) 351, 398, 806, 1481, 2043, 2353, 2366
AlwNI CAGNNNCTG 1 cut(s) 821
Ama87I CYCGRG 3 cut(s) 10, 504, 2396
Aor13HI TCCGGA 1 cut(s) 2355
Aor51HI AGCGCT 1 cut(s) 1597
AoxI GGCC 3 cut(s) 1659, 2309, 2400
ApeKI GCWGC 4 cut(s) 5, 88, 1691, 1935
ApoI RAATTY 3 cut(s) 410, 1829, 2149
AsiGI ACCGGT 1 cut(s) 1886
Asp700I GAANNNNTTC 1 cut(s) 1630
AspLEI GCGC 5 cut(s) 169, 548, 1277, 1349, 1598
AspS9I GGNCC 3 cut(s) 232, 427, 2309
AsuHPI GGTGA 8 cut(s) 662, 986, 1427, 1550, 1901, 2039, 2180, 2459
AsuII TTCGAA 1 cut(s) 2070
AvaI CYCGRG 3 cut(s) 10, 504, 2396
AvaII GGWCC 2 cut(s) 232, 427
BalI TGGCCA 1 cut(s) 1661
BamHI GGATCC 1 cut(s) 2358
BauI CACGAG 2 cut(s) 437, 615
BbvI GCAGC 3 cut(s) 75, 1703, 1947
BccI CCATC 4 cut(s) 989, 1009, 1505, 1723
BceAI ACGGC 1 cut(s) 267
BciT130I CCWGG 2 cut(s) 47, 2489
BciVI GTATCC 2 cut(s) 1426, 1857
BclI TGATCA 2 cut(s) 1075, 2376
BcoDI GTCTC 4 cut(s) 362, 771, 850, 1874
BfaI CTAG 3 cut(s) 1251, 1440, 1865
BfmI CTRYAG 2 cut(s) 888, 2319
BfoI RGCGCY 3 cut(s) 170, 549, 1599
BfuI GTATCC 2 cut(s) 1426, 1857
BglII AGATCT 2 cut(s) 1810, 2323
BisI GCNGC 5 cut(s) 6, 89, 1692, 1936, 2013
BlsI GCNGC 5 cut(s) 7, 90, 1693, 1937, 2014
Bme1390I CCNGG 2 cut(s) 47, 2489
Bme18I GGWCC 2 cut(s) 232, 427
BmeT110I CYCGRG 3 cut(s) 10, 504, 2396
BmgT120I GGNCC 3 cut(s) 232, 427, 2309
BmiI GGNNCC 5 cut(s) 233, 429, 2011, 2191, 2360
BmrFI CCNGG 2 cut(s) 47, 2489
BmsI GCATC 4 cut(s) 541, 827, 902, 1851
BpmI CTGGAG 2 cut(s) 941, 1697
Bpu14I TTCGAA 1 cut(s) 2070
BpuEI CTTGAG 2 cut(s) 686, 1280
Bsa29I ATCGAT 2 cut(s) 1555, 1859
BsaBI GATNNNNATC 1 cut(s) 2087
BsaI GGTCTC 1 cut(s) 850
BsaJI CCNNGG 9 cut(s) 9, 45, 494, 605, 786, 874, 913, 1506, 2488
BsaWI WCCGGW 2 cut(s) 1886, 2355
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 919, 1254, 1512
Bse118I RCCGGY 1 cut(s) 1886
Bse1I ACTGG 4 cut(s) 387, 924, 938, 1063
Bse3DI GCAATG 4 cut(s) 1020, 1320, 1356, 1662
Bse8I GATNNNNATC 1 cut(s) 2087
BseAI TCCGGA 1 cut(s) 2355
BseBI CCWGG 2 cut(s) 47, 2489
BseCI ATCGAT 2 cut(s) 1555, 1859
BseDI CCNNGG 9 cut(s) 9, 45, 494, 605, 786, 874, 913, 1506, 2488
BseGI GGATG 2 cut(s) 406, 1516
BseJI GATNNNNATC 1 cut(s) 2087
BseLI CCNNNNNNNGG 4 cut(s) 51, 919, 1254, 1512
BseMI GCAATG 4 cut(s) 1020, 1320, 1356, 1662
BseMII CTCAG 2 cut(s) 2282, 2354
BseNI ACTGG 4 cut(s) 387, 924, 938, 1063
BseRI GAGGAG 2 cut(s) 1697, 2465
BseXI GCAGC 3 cut(s) 75, 1703, 1947
BsgI GTGCAG 1 cut(s) 960
Bsh1285I CGRYCG 1 cut(s) 2407
BshFI GGCC 3 cut(s) 1661, 2311, 2402
BshTI ACCGGT 1 cut(s) 1886
BshVI ATCGAT 2 cut(s) 1555, 1859
BsiEI CGRYCG 1 cut(s) 2407
BsiHKCI CYCGRG 3 cut(s) 10, 504, 2396
BsiSI CCGG 2 cut(s) 1887, 2356
BslFI GGGAC 6 cut(s) 28, 245, 856, 2256, 2298, 2469
BslI CCNNNNNNNGG 4 cut(s) 51, 919, 1254, 1512
BsmAI GTCTC 4 cut(s) 362, 771, 850, 1874
BsmFI GGGAC 6 cut(s) 28, 245, 856, 2256, 2298, 2469
BsmI GAATGC 2 cut(s) 511, 1458
BsnI GGCC 3 cut(s) 1661, 2311, 2402
Bso31I GGTCTC 1 cut(s) 850
BsoBI CYCGRG 3 cut(s) 10, 504, 2396
Bsp119I TTCGAA 1 cut(s) 2070
Bsp13I TCCGGA 1 cut(s) 2355
Bsp1407I TGTACA 2 cut(s) 1107, 1957
Bsp19I CCATGG 3 cut(s) 494, 874, 913
BspACI CCGC 4 cut(s) 375, 1091, 1820, 2013
BspANI GGCC 3 cut(s) 1661, 2311, 2402
BspCNI CTCAG 2 cut(s) 2281, 2355
BspDI ATCGAT 2 cut(s) 1555, 1859
BspEI TCCGGA 1 cut(s) 2355
BspHI TCATGA 1 cut(s) 769
BspLI GGNNCC 5 cut(s) 233, 429, 2011, 2191, 2360
BspMAI CTGCAG 1 cut(s) 2323
BspPI GGATC 7 cut(s) 351, 398, 806, 1481, 2043, 2353, 2366
BspQI GCTCTTC 2 cut(s) 463, 1728
BspT104I TTCGAA 1 cut(s) 2070
BspTNI GGTCTC 1 cut(s) 850
BsrDI GCAATG 4 cut(s) 1020, 1320, 1356, 1662
BsrFI RCCGGY 1 cut(s) 1886
BsrGI TGTACA 2 cut(s) 1107, 1957
BsrI ACTGG 4 cut(s) 387, 924, 938, 1063
BssAI RCCGGY 1 cut(s) 1886
BssECI CCNNGG 9 cut(s) 9, 45, 494, 605, 786, 874, 913, 1506, 2488
BssSI CACGAG 2 cut(s) 437, 615
BssT1I CCWWGG 4 cut(s) 494, 786, 874, 913
Bst2BI CACGAG 2 cut(s) 437, 615
Bst2UI CCWGG 2 cut(s) 47, 2489
Bst4CI ACNGT 6 cut(s) 155, 901, 1239, 2097, 2289, 2447
Bst6I CTCTTC 5 cut(s) 338, 463, 1204, 1728, 2217
BstAUI TGTACA 2 cut(s) 1107, 1957
BstBI TTCGAA 1 cut(s) 2070
BstC8I GCNNGC 2 cut(s) 165, 1740
BstDEI CTNAG 4 cut(s) 867, 1721, 2268, 2363
BstDSI CCRYGG 4 cut(s) 494, 874, 913, 1506
BstF5I GGATG 2 cut(s) 406, 1516
BstH2I RGCGCY 3 cut(s) 170, 549, 1599
BstHHI GCGC 5 cut(s) 169, 548, 1277, 1349, 1598
BstMAI GTCTC 4 cut(s) 362, 771, 850, 1874
BstMCI CGRYCG 1 cut(s) 2407
BstMWI GCNNNNNNNGC 3 cut(s) 175, 462, 2399
BstNI CCWGG 2 cut(s) 47, 2489
BstNSI RCATGY 2 cut(s) 1970, 2426
BstSCI CCNGG 2 cut(s) 45, 2487
BstSFI CTRYAG 2 cut(s) 888, 2319
BstV1I GCAGC 3 cut(s) 75, 1703, 1947
BstX2I RGATCY 6 cut(s) 356, 811, 1473, 1810, 2323, 2358
BstYI RGATCY 6 cut(s) 356, 811, 1473, 1810, 2323, 2358
Bsu15I ATCGAT 2 cut(s) 1555, 1859
BsuI GTATCC 2 cut(s) 1426, 1857
BsuRI GGCC 3 cut(s) 1661, 2311, 2402
BsuTUI ATCGAT 2 cut(s) 1555, 1859
BtgI CCRYGG 4 cut(s) 494, 874, 913, 1506
BtsCI GGATG 2 cut(s) 406, 1516
BtsI GCAGTG 1 cut(s) 2311
BtsIMutI CAGTG 3 cut(s) 1531, 2294, 2311
Cac8I GCNNGC 2 cut(s) 165, 1740
CaiI CAGNNNCTG 1 cut(s) 821
CciI TCATGA 1 cut(s) 769
CfoI GCGC 5 cut(s) 169, 548, 1277, 1349, 1598
Cfr10I RCCGGY 1 cut(s) 1886
Cfr13I GGNCC 3 cut(s) 232, 427, 2309
ClaI ATCGAT 2 cut(s) 1555, 1859
CseI GACGC 2 cut(s) 40, 1553
Csp6I GTAC 6 cut(s) 68, 285, 882, 1108, 1805, 1958
CspAI ACCGGT 1 cut(s) 1886
CviQI GTAC 6 cut(s) 68, 285, 882, 1108, 1805, 1958
DdeI CTNAG 4 cut(s) 867, 1721, 2268, 2363
DrdI GACNNNNNNGTC 1 cut(s) 1784
DseDI GACNNNNNNGTC 1 cut(s) 1784
EaeI YGGCCR 1 cut(s) 1659
Eam1104I CTCTTC 5 cut(s) 338, 463, 1204, 1728, 2217
EarI CTCTTC 5 cut(s) 338, 463, 1204, 1728, 2217
EciI GGCGGA 1 cut(s) 1106
Eco130I CCWWGG 4 cut(s) 494, 786, 874, 913
Eco31I GGTCTC 1 cut(s) 850
Eco47I GGWCC 2 cut(s) 232, 427
Eco47III AGCGCT 1 cut(s) 1597
Eco57I CTGAAG 2 cut(s) 1233, 1629
Eco88I CYCGRG 3 cut(s) 10, 504, 2396
EcoO109I RGGNCCY 2 cut(s) 232, 427
EcoRII CCWGG 2 cut(s) 45, 2487
EcoT14I CCWWGG 4 cut(s) 494, 786, 874, 913
ErhI CCWWGG 4 cut(s) 494, 786, 874, 913
FalI AAGNNNNNCTT 4 cut(s) 1615, 1647, 2043, 2075
FaqI GGGAC 6 cut(s) 28, 245, 856, 2256, 2298, 2469
FauNDI CATATG 2 cut(s) 127, 651
FbaI TGATCA 2 cut(s) 1075, 2376
FblI GTMKAC 1 cut(s) 1244
Fnu4HI GCNGC 5 cut(s) 6, 89, 1692, 1936, 2013
FokI GGATG 2 cut(s) 413, 1523
Fsp4HI GCNGC 5 cut(s) 6, 89, 1692, 1936, 2013
FspBI CTAG 3 cut(s) 1251, 1440, 1865
GlaI GCGC 5 cut(s) 168, 547, 1276, 1348, 1597
GluI GCNGC 5 cut(s) 6, 89, 1692, 1936, 2013
GsuI CTGGAG 2 cut(s) 941, 1697
HaeII RGCGCY 3 cut(s) 170, 549, 1599
HaeIII GGCC 3 cut(s) 1661, 2311, 2402
HapII CCGG 2 cut(s) 1887, 2356
HgaI GACGC 2 cut(s) 40, 1553
HhaI GCGC 5 cut(s) 169, 548, 1277, 1349, 1598
Hin6I GCGC 5 cut(s) 167, 546, 1275, 1347, 1596
HinP1I GCGC 5 cut(s) 167, 546, 1275, 1347, 1596
HincII GTYRAC 3 cut(s) 709, 972, 988
HindII GTYRAC 3 cut(s) 709, 972, 988
HindIII AAGCTT 3 cut(s) 60, 296, 1425
HpaI GTTAAC 1 cut(s) 709
HpaII CCGG 2 cut(s) 1887, 2356
HphI GGTGA 8 cut(s) 662, 986, 1427, 1550, 1901, 2039, 2180, 2459
Hpy166II GTNNAC 7 cut(s) 70, 112, 709, 972, 988, 1245, 1958
Hpy8I GTNNAC 7 cut(s) 70, 112, 709, 972, 988, 1245, 1958
HpyCH4III ACNGT 6 cut(s) 155, 901, 1239, 2097, 2289, 2447
HpyCH4IV ACGT 3 cut(s) 470, 521, 2462
HpyF10VI GCNNNNNNNGC 3 cut(s) 175, 462, 2399
HpyF3I CTNAG 4 cut(s) 867, 1721, 2268, 2363
HpySE526I ACGT 3 cut(s) 470, 521, 2462
HspAI GCGC 5 cut(s) 167, 546, 1275, 1347, 1596
Kpn2I TCCGGA 1 cut(s) 2355
Ksp22I TGATCA 2 cut(s) 1075, 2376
KspAI GTTAAC 1 cut(s) 709
LguI GCTCTTC 2 cut(s) 463, 1728
LmnI GCTCC 4 cut(s) 808, 1515, 1822, 2009
Lsp1109I GCAGC 3 cut(s) 75, 1703, 1947
LweI GCATC 4 cut(s) 541, 827, 902, 1851
MaeI CTAG 3 cut(s) 1251, 1440, 1865
MaeII ACGT 3 cut(s) 470, 521, 2462
MaeIII GTNAC 5 cut(s) 1207, 1233, 2027, 2118, 2335
MfeI CAATTG 1 cut(s) 1026
MflI RGATCY 6 cut(s) 356, 811, 1473, 1810, 2323, 2358
MlsI TGGCCA 1 cut(s) 1661
MluNI TGGCCA 1 cut(s) 1661
MlyI GAGTC 7 cut(s) 1190, 1291, 1327, 1363, 1399, 1502, 2234
MmeI TCCRAC 2 cut(s) 925, 1593
Mox20I TGGCCA 1 cut(s) 1661
MroI TCCGGA 1 cut(s) 2355
MroXI GAANNNNTTC 1 cut(s) 1630
MscI TGGCCA 1 cut(s) 1661
MseI TTAA 7 cut(s) 351, 708, 930, 1559, 2259, 2430, 2439
MslI CAYNNNNRTG 2 cut(s) 650, 1965
Msp20I TGGCCA 1 cut(s) 1661
MspI CCGG 2 cut(s) 1887, 2356
MspR9I CCNGG 2 cut(s) 47, 2489
MunI CAATTG 1 cut(s) 1026
Mva1269I GAATGC 2 cut(s) 511, 1458
MvaI CCWGG 2 cut(s) 47, 2489
MwoI GCNNNNNNNGC 3 cut(s) 175, 462, 2399
NcoI CCATGG 3 cut(s) 494, 874, 913
NdeI CATATG 2 cut(s) 127, 651
NlaIV GGNNCC 5 cut(s) 233, 429, 2011, 2191, 2360
NmeAIII GCCGAG 2 cut(s) 391, 1768
NmuCI GTSAC 3 cut(s) 1233, 2027, 2118
NspI RCATGY 2 cut(s) 1970, 2426
NspV TTCGAA 1 cut(s) 2070
PaeR7I CTCGAG 1 cut(s) 2396
PagI TCATGA 1 cut(s) 769
PciI ACATGT 1 cut(s) 1966
PciSI GCTCTTC 2 cut(s) 463, 1728
PcsI WCGNNNNNNNCGW 1 cut(s) 1561
PctI GAATGC 2 cut(s) 511, 1458
PdmI GAANNNNTTC 1 cut(s) 1630
PflMI CCANNNNNTGG 1 cut(s) 919
PinAI ACCGGT 1 cut(s) 1886
PkrI GCNGC 5 cut(s) 7, 90, 1693, 1937, 2014
PleI GAGTC 7 cut(s) 1190, 1290, 1326, 1362, 1398, 1501, 2233
PpsI GAGTC 7 cut(s) 1190, 1290, 1326, 1362, 1398, 1501, 2233
PpuMI RGGWCCY 2 cut(s) 232, 427
PscI ACATGT 1 cut(s) 1966
Psp1406I AACGTT 2 cut(s) 470, 521
Psp5II RGGWCCY 2 cut(s) 232, 427
Psp6I CCWGG 2 cut(s) 45, 2487
PspGI CCWGG 2 cut(s) 45, 2487
PspN4I GGNNCC 5 cut(s) 233, 429, 2011, 2191, 2360
PspPI GGNCC 3 cut(s) 232, 427, 2309
PspPPI RGGWCCY 2 cut(s) 232, 427
PspXI VCTCGAGB 1 cut(s) 2396
PstI CTGCAG 1 cut(s) 2323
PstNI CAGNNNCTG 1 cut(s) 821
PsuI RGATCY 6 cut(s) 356, 811, 1473, 1810, 2323, 2358
RsaI GTAC 6 cut(s) 69, 286, 883, 1109, 1806, 1959
RsaNI GTAC 6 cut(s) 68, 285, 882, 1108, 1805, 1958
RseI CAYNNNNRTG 2 cut(s) 650, 1965
SapI GCTCTTC 2 cut(s) 463, 1728
SaqAI TTAA 7 cut(s) 351, 708, 930, 1559, 2259, 2430, 2439
SatI GCNGC 5 cut(s) 6, 89, 1692, 1936, 2013
Sau96I GGNCC 3 cut(s) 232, 427, 2309
SchI GAGTC 7 cut(s) 1190, 1291, 1327, 1363, 1399, 1502, 2234
ScrFI CCNGG 2 cut(s) 47, 2489
SfaNI GCATC 4 cut(s) 541, 827, 902, 1851
SfcI CTRYAG 2 cut(s) 888, 2319
Sfr274I CTCGAG 1 cut(s) 2396
SfuI TTCGAA 1 cut(s) 2070
SinI GGWCC 2 cut(s) 232, 427
SlaI CTCGAG 1 cut(s) 2396
SmiMI CAYNNNNRTG 2 cut(s) 650, 1965
SmlI CTYRAG 3 cut(s) 701, 1259, 2396
SmoI CTYRAG 3 cut(s) 701, 1259, 2396
SsiI CCGC 4 cut(s) 375, 1091, 1820, 2013
SspI AATATT 1 cut(s) 540
SspMI CTAG 3 cut(s) 1251, 1440, 1865
StyD4I CCNGG 2 cut(s) 45, 2487
StyI CCWWGG 4 cut(s) 494, 786, 874, 913
TaaI ACNGT 6 cut(s) 155, 901, 1239, 2097, 2289, 2447
TaiI ACGT 3 cut(s) 473, 524, 2465
TaqI TCGA 7 cut(s) 420, 561, 1555, 1788, 1859, 2070, 2397
TaqII GACCGA 1 cut(s) 2421
TatI WGTACW 4 cut(s) 881, 1107, 1804, 1957
TauI GCSGC 1 cut(s) 2015
Tru1I TTAA 7 cut(s) 351, 708, 930, 1559, 2259, 2430, 2439
Tru9I TTAA 7 cut(s) 351, 708, 930, 1559, 2259, 2430, 2439
TscAI CASTG 3 cut(s) 1531, 2294, 2311
TseFI GTSAC 3 cut(s) 1233, 2027, 2118
TseI GCWGC 4 cut(s) 5, 88, 1691, 1935
Tsp45I GTSAC 3 cut(s) 1233, 2027, 2118
TspGWI ACGGA 4 cut(s) 957, 1495, 1688, 2200
TspRI CASTG 3 cut(s) 1531, 2294, 2311
Van91I CCANNNNNTGG 1 cut(s) 919
VpaK11BI GGWCC 2 cut(s) 232, 427
XapI RAATTY 3 cut(s) 410, 1829, 2149
XceI RCATGY 2 cut(s) 1970, 2426
XhoI CTCGAG 1 cut(s) 2396
XmiI GTMKAC 1 cut(s) 1244
XmnI GAANNNNTTC 1 cut(s) 1630
XspI CTAG 3 cut(s) 1251, 1440, 1865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.