Rh3CG027100

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0007026
Physical Location & Seq
Reverse (-)
1889935 .. 1894891
4957 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG027100.1

Sequence Viewer

Length: 2778 bp
ATGGCTGCCCCGGGGACTATGTCTACTAATCATCACAAAGTTCCTTGGGGTCCGGCATTGGTGTCAGCTAGTCTCGAGTGGTTACTGATTTTTATGCTGGTTGTGGATGCCATATTCTCTTATGTAATTACGAAGTTTGCTCATTACTGGGGATTGCAAACACCATGCCTCCTGTGCTCCAGACTTGATCATGTCTTGGGCAATGAAAAACCAGGTTATTATTGGGACTTGATCTGCGGCAGTCATAAGTCAGAGATTTCTTCCTTAGTTCTTTGTCGTGCACACAACAAGCTTGTAGATGTTAATGGAATGTGTGAAAGTTGCCTATTCTCTTTTGCAACCATTAATAGATCCAATGCTGAGACATACAGGTTATTGGTGGGCAAGTTGGGGGAAGCTCCTAATTCTAAGGCTGACCATGAGATTGAGGACCCAGTACTCGGGGGCCAATATGTTTGCTTTTCCGGCAGAGCACATTGTTCTTGTTGCAATGAGCCGCAAATTTCTGAGGGGTATTCTCATAAGTTGATTCACTCTAAATTGTTTGGGTCTGAGGCTGAACTTGAGCCCCTATCTGGTGCTATTAGACAAAACCTTGAAGAACTGAGGAAGGCACAAGATAAATCATCTAGGTCATTGAGAGCTTCTCATTTGAGAGATACTGGGCTCCCTTTATCTCATGTTGGATACACAGAGCTCAAAGGTTCTGACACTGAATCAGAAGTTCATTTTTCTGACGGGGAGGATGCAAGTGCTTCAATTCATGAAAAGGGAAAAGGTGTTGAACCTCATACTATTACTCCGGTTCTGATAGATCCACCTTCAGTGTCCAAGACTTTAGTTTCAGCATCAGAGATGACATTAGATAATATGGAGTCCCATGGTAATACATCTGCTCGTGTGAGAGATATTAGGCTTCATGCTTTATCTCATGTCGGATACACAGAGCTTAAGGTCACTTCAGATACTGAATCGGAAGTTCATTTCTCTGATGATGATTATGCAGGTTCTCTGACTCATGAAATGGATGGTCCCAAGGAAGATTCCTCTGCTCAAGCTGTTGAGCCTCATGTTATTCCTCAAGTACTAACAGATCCACCATCTGTGTCCAAGCCCTCACTTTTGGTATCACAGGCACAAATAGATCGTATAGAGTCCCTAGGTAGTACATCTGTAGCATCTGCAGTTACTATTGGTCATGGATTGGAGGAACTTAACTTGAAAGAAGCTAGAAGAACACCTGATTATCATGCCTTATCTGAACCACTACTTGAGAATGCCTCTCCATCATCAAAGGCAATGGAAGCCCCAGTTGAAGTGTCAAAGAGAAGCTTAGATGTTACAGGAACTCGTGAAATTGATCAAGCATATGTAGCAGAATCTGGGGAATTTCACAAGAGAGCTGTCAGGCCACTTAAAACATCTGAAGTACGTTTAGAAACAAACGCAGTTTCAAGCAACACTGATCAGCAAGTGGTTAATGTTTTAGATCTCGGTGATGCTTATAAGATCGTTGTTGGTAGTCAAGGAAGACAATTGCCAGGTGTACTTGCGGAACAATGGATTGGAAAGGACTCTTCAAGAGTTACTGAAGATTTGAAGATCCTGTTGTCACAATTGTCTGCTAATCGAGGGATTGAGCAATCAACAAATGAAATTATCAGTCCAACAAATAGTGGTGATCTGAAAACTATGGGGATGCAGATACGTCAACAGAGGATCTCACTAGAAAGAAATGAGTCTGGGTTGTCTATTGATGGGAGCACTGTCAGTGAAATTGAGGGTGAGAGTATGCTTGATCGATTAAAACGACAGGTTGAGCATGACAAGAAACTTTTGAGTTCTTTGTACAAGGAGTTGGAGGAAGAAAGAAATGCTTCTGCGATTTCTTCAGATCAAGCAATGGCCATGATTACAAGGCTGCAAGAGGAGAAGGCGGCATTTCATATGGAAGCACTTCAGTCCCTAAGAATGATGGAAGAGCAAGCTGAGTATGATAATGAAGCACTGCATAAGATAGATGACCTTCTTGCTGAAAAGGAGAAGGAAGTACAAGATCTAGAAGCAGAGCTAGAATTTTACAGGATTAAGTACCCAAATGAATCAATGCTAGAGTGTCTGGGAGAGACAACTTCTGATATGCAGGCTAGGGATATTGTGGTTGATCATTCAGTACCTAGCAGCCTTGCAGAGAGTCATAAAAATACGGATGCTGGAAAGCCTTGTACAGACAGTAAAGATGGAGGAGCGGGGATGATTCTCAGTGATGAGAAAACTGGTAGTTTAAAGAACTCACTGAAGGATTTTGAGGTAGAGAAGAAACAGATCTTGCAATGCTTAGAGAAGTTGGAGAAGACACTTTCTTTGTTCTCTAGTAATGGGGTACAGACGGATTCATCTAAAGGAAATTGTTCTGAAAATGGAGGAGATGGGGCTGGCAAGTCAAATCTGCAGAATTCCGAACAGCACTCTCAAGAAAATATTAAGAAAGAAGAAAATGATTTTTCCAAGCAACATGAAGTGCCTGCATTGTCGGGACATAGTTCACCACACGAGATCCCCCAGCCCTGTCAAGCGAATGATTTAGCATCTCTATCGGTTCTGGCTTTCAATCTGAATAAGAGATTGAAGGCAATTGAGGCAGTCCTAGAATTTCTTGAACAAGCAATTACCTCTCTCAAATATGGTGAGGAGGCACTGAAGTTCATTCAGGAGATCGCTTCTCACCTAGCGGAACTACGGAAGATTGGGATTAGAAAGGATCAGACTTCAGCTTGA

Protein Analysis

925

Amino Acids

101.64

Weight (kDa)

5.12

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 601 - 691 9.4e-29 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1506
Acc36I ACCTGC 1 cut(s) 995
AccBSI CCGCTC 1 cut(s) 2249
AccI GTMKAC 1 cut(s) 23
AciI CCGC 6 cut(s) 237, 497, 1553, 1937, 2249, 2732
AclWI GGATC 7 cut(s) 345, 809, 1088, 1597, 1727, 2551, 2769
AcoI YGGCCR 1 cut(s) 1905
AcsI RAATTY 5 cut(s) 501, 1388, 2075, 2455, 2651
AcuI CTGAAG 9 cut(s) 807, 945, 1446, 1611, 1875, 1943, 2318, 2720, 2754
AfiI CCNNNNNNNGG 2 cut(s) 440, 575
AflII CTTAAG 1 cut(s) 950
AjnI CCWGG 2 cut(s) 211, 1540
AjuI GAANNNNNNNTTGG 2 cut(s) 1548, 1580
Alw21I GWGCWC 5 cut(s) 179, 283, 475, 699, 1766
Alw26I GTCTC 3 cut(s) 77, 356, 2120
Alw44I GTGCAC 1 cut(s) 279
AlwI GGATC 7 cut(s) 345, 809, 1088, 1597, 1727, 2551, 2769
AlwNI CAGNNNCTG 2 cut(s) 968, 1382
Ama87I CYCGRG 3 cut(s) 10, 74, 440
AoxI GGCC 3 cut(s) 445, 1409, 1905
ApaLI GTGCAC 1 cut(s) 279
ApeKI GCWGC 3 cut(s) 5, 1921, 2181
ApoI RAATTY 5 cut(s) 501, 1388, 2075, 2455, 2651
ArsI GACNNNNNNTTYG 2 cut(s) 2347, 2379
AseI ATTAAT 1 cut(s) 345
Asp700I GAANNNNTTC 3 cut(s) 1876, 1956, 2410
AspA2I CCTAGG 1 cut(s) 1159
AspS9I GGNCC 4 cut(s) 50, 430, 445, 1031
AsuC2I CCSGG 2 cut(s) 11, 12
AsuHPI GGTGA 6 cut(s) 1508, 1691, 1796, 2538, 2699, 2717
AvaI CYCGRG 3 cut(s) 10, 74, 440
AvaII GGWCC 3 cut(s) 50, 430, 1031
AvrII CCTAGG 1 cut(s) 1159
BaeGI GKGCMC 1 cut(s) 283
BalI TGGCCA 1 cut(s) 1907
BanII GRGCYC 3 cut(s) 570, 669, 699
BauI CACGAG 3 cut(s) 897, 1350, 2552
BbsI GAAGAC 2 cut(s) 1537, 2360
Bbv12I GWGCWC 5 cut(s) 179, 283, 475, 699, 1766
BbvI GCAGC 2 cut(s) 1908, 2193
BccI CCATC 7 cut(s) 1022, 1108, 1294, 1751, 1969, 2234, 2423
BcgI CGANNNNNNTGC 2 cut(s) 2577, 2611
BciT130I CCWGG 2 cut(s) 213, 1542
BciVI GTATCC 2 cut(s) 680, 932
BclI TGATCA 4 cut(s) 187, 1360, 1465, 2164
BcnI CCSGG 2 cut(s) 11, 12
BcoDI GTCTC 3 cut(s) 77, 356, 2120
BfmI CTRYAG 3 cut(s) 1173, 1182, 2450
BfrI CTTAAG 1 cut(s) 950
BfuAI ACCTGC 1 cut(s) 995
BfuI GTATCC 2 cut(s) 680, 932
BglII AGATCT 3 cut(s) 1489, 2056, 2325
BisI GCNGC 6 cut(s) 6, 238, 497, 1922, 1938, 2182
BlnI CCTAGG 1 cut(s) 1159
BlsI GCNGC 6 cut(s) 7, 239, 498, 1923, 1939, 2183
BmcAI AGTACT 2 cut(s) 438, 1086
Bme1390I CCNGG 4 cut(s) 11, 12, 213, 1542
Bme18I GGWCC 3 cut(s) 50, 430, 1031
BmeT110I CYCGRG 3 cut(s) 10, 74, 440
BmgT120I GGNCC 4 cut(s) 50, 430, 445, 1031
BmiI GGNNCC 5 cut(s) 51, 432, 446, 668, 1033
BmrFI CCNGG 4 cut(s) 11, 12, 213, 1542
BmrI ACTGGG 4 cut(s) 157, 428, 672, 1304
BmsI GCATC 8 cut(s) 97, 736, 857, 1187, 1489, 1689, 2200, 2597
BmuI ACTGGG 4 cut(s) 157, 428, 672, 1304
BpiI GAAGAC 2 cut(s) 1537, 2360
BplI GAGNNNNNCTC 4 cut(s) 631, 663, 1265, 1297
BpmI CTGGAG 1 cut(s) 163
BpuEI CTTGAG 5 cut(s) 584, 1038, 1065, 1292, 2457
BpuMI CCSGG 2 cut(s) 11, 12
Bsa29I ATCGAT 1 cut(s) 1801
BsaJI CCNNGG 7 cut(s) 9, 10, 11, 44, 880, 1035, 1159
BsaWI WCCGGW 1 cut(s) 802
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 784, 814
Bsc4I CCNNNNNNNGG 2 cut(s) 440, 575
Bse1I ACTGG 5 cut(s) 152, 434, 667, 1310, 2281
Bse3DI GCAATG 5 cut(s) 208, 496, 1305, 1908, 2339
BseBI CCWGG 2 cut(s) 213, 1542
BseCI ATCGAT 1 cut(s) 1801
BseDI CCNNGG 7 cut(s) 9, 10, 11, 44, 880, 1035, 1159
BseGI GGATG 6 cut(s) 112, 751, 1033, 1704, 2215, 2259
BseLI CCNNNNNNNGG 2 cut(s) 440, 575
BseMI GCAATG 5 cut(s) 208, 496, 1305, 1908, 2339
BseMII CTCAG 6 cut(s) 351, 498, 543, 596, 1980, 2275
BseNI ACTGG 5 cut(s) 152, 434, 667, 1310, 2281
BseRI GAGGAG 4 cut(s) 1943, 2259, 2439, 2705
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 2 cut(s) 1908, 2193
BseYI CCCAGC 1 cut(s) 2562
BshFI GGCC 3 cut(s) 447, 1411, 1907
BshVI ATCGAT 1 cut(s) 1801
BsiHKAI GWGCWC 5 cut(s) 179, 283, 475, 699, 1766
BsiHKCI CYCGRG 3 cut(s) 10, 74, 440
BsiSI CCGG 4 cut(s) 11, 53, 465, 803
BslFI GGGAC 7 cut(s) 28, 239, 862, 1017, 1141, 1948, 2550
BslI CCNNNNNNNGG 2 cut(s) 440, 575
BsmAI GTCTC 3 cut(s) 77, 356, 2120
BsmFI GGGAC 7 cut(s) 28, 239, 862, 1017, 1141, 1948, 2550
BsmI GAATGC 1 cut(s) 1282
BsnI GGCC 3 cut(s) 447, 1411, 1907
BsoBI CYCGRG 3 cut(s) 10, 74, 440
Bsp1286I GDGCHC 7 cut(s) 179, 283, 475, 570, 669, 699, 1766
Bsp1407I TGTACA 2 cut(s) 1848, 2225
Bsp19I CCATGG 1 cut(s) 880
BspACI CCGC 6 cut(s) 237, 497, 1553, 1937, 2249, 2732
BspANI GGCC 3 cut(s) 447, 1411, 1907
BspCNI CTCAG 6 cut(s) 352, 499, 544, 597, 1981, 2274
BspDI ATCGAT 1 cut(s) 1801
BspHI TCATGA 2 cut(s) 763, 1018
BspLI GGNNCC 5 cut(s) 51, 432, 446, 668, 1033
BspMAI CTGCAG 2 cut(s) 1186, 2454
BspMI ACCTGC 1 cut(s) 995
BspPI GGATC 7 cut(s) 345, 809, 1088, 1597, 1727, 2551, 2769
BspQI GCTCTTC 1 cut(s) 1974
BspTI CTTAAG 1 cut(s) 950
BsrBI CCGCTC 1 cut(s) 2249
BsrDI GCAATG 5 cut(s) 208, 496, 1305, 1908, 2339
BsrGI TGTACA 2 cut(s) 1848, 2225
BsrI ACTGG 5 cut(s) 152, 434, 667, 1310, 2281
BssECI CCNNGG 7 cut(s) 9, 10, 11, 44, 880, 1035, 1159
BssSI CACGAG 3 cut(s) 897, 1350, 2552
BssT1I CCWWGG 4 cut(s) 44, 880, 1035, 1159
Bst2BI CACGAG 3 cut(s) 897, 1350, 2552
Bst2UI CCWGG 2 cut(s) 213, 1542
Bst4CI ACNGT 2 cut(s) 1768, 2234
Bst6I CTCTTC 2 cut(s) 1582, 1974
BstAFI CTTAAG 1 cut(s) 950
BstAUI TGTACA 2 cut(s) 1848, 2225
BstC8I GCNNGC 4 cut(s) 1986, 2145, 2437, 2526
BstDSI CCRYGG 1 cut(s) 880
BstF5I GGATG 6 cut(s) 112, 751, 1033, 1704, 2215, 2259
BstMAI GTCTC 3 cut(s) 77, 356, 2120
BstMWI GCNNNNNNNGC 5 cut(s) 174, 465, 1304, 1373, 2639
BstNI CCWGG 2 cut(s) 213, 1542
BstSCI CCNGG 4 cut(s) 9, 10, 211, 1540
BstSFI CTRYAG 3 cut(s) 1173, 1182, 2450
BstSLI GKGCMC 1 cut(s) 283
BstV1I GCAGC 2 cut(s) 1908, 2193
BstV2I GAAGAC 2 cut(s) 1537, 2360
BstX2I RGATCY 9 cut(s) 350, 814, 1093, 1489, 1602, 1719, 2056, 2325, 2556
BstYI RGATCY 9 cut(s) 350, 814, 1093, 1489, 1602, 1719, 2056, 2325, 2556
Bsu15I ATCGAT 1 cut(s) 1801
BsuI GTATCC 2 cut(s) 680, 932
BsuRI GGCC 3 cut(s) 447, 1411, 1907
BsuTUI ATCGAT 1 cut(s) 1801
BtgI CCRYGG 1 cut(s) 880
BtsCI GGATG 6 cut(s) 112, 751, 1033, 1704, 2215, 2259
BtsI GCAGTG 1 cut(s) 2006
BtsIMutI CAGTG 9 cut(s) 711, 831, 1461, 1764, 1777, 2006, 2269, 2294, 2696
BveI ACCTGC 1 cut(s) 995
Cac8I GCNNGC 4 cut(s) 1986, 2145, 2437, 2526
CaiI CAGNNNCTG 2 cut(s) 968, 1382
CciI TCATGA 2 cut(s) 763, 1018
Cfr13I GGNCC 4 cut(s) 50, 430, 445, 1031
Cfr9I CCCGGG 1 cut(s) 10
ClaI ATCGAT 1 cut(s) 1801
CsiI ACCWGGT 1 cut(s) 211
DraI TTTAAA 1 cut(s) 2286
EaeI YGGCCR 1 cut(s) 1905
Eam1104I CTCTTC 2 cut(s) 1582, 1974
EarI CTCTTC 2 cut(s) 1582, 1974
Ecl136II GAGCTC 1 cut(s) 697
Eco130I CCWWGG 4 cut(s) 44, 880, 1035, 1159
Eco24I GRGCYC 3 cut(s) 570, 669, 699
Eco47I GGWCC 3 cut(s) 50, 430, 1031
Eco53kI GAGCTC 1 cut(s) 697
Eco57I CTGAAG 9 cut(s) 807, 945, 1446, 1611, 1875, 1943, 2318, 2720, 2754
Eco88I CYCGRG 3 cut(s) 10, 74, 440
EcoICRI GAGCTC 1 cut(s) 697
EcoO109I RGGNCCY 1 cut(s) 430
EcoRI GAATTC 1 cut(s) 2455
EcoRII CCWGG 2 cut(s) 211, 1540
EcoT14I CCWWGG 4 cut(s) 44, 880, 1035, 1159
EcoT38I GRGCYC 3 cut(s) 570, 669, 699
ErhI CCWWGG 4 cut(s) 44, 880, 1035, 1159
FalI AAGNNNNNCTT 4 cut(s) 1316, 1348, 1861, 1893
FaqI GGGAC 7 cut(s) 28, 239, 862, 1017, 1141, 1948, 2550
FauI CCCGC 1 cut(s) 2242
FauNDI CATATG 2 cut(s) 1369, 1947
FbaI TGATCA 4 cut(s) 187, 1360, 1465, 2164
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 6 cut(s) 6, 238, 497, 1922, 1938, 2182
FokI GGATG 6 cut(s) 119, 758, 1040, 1711, 2222, 2266
FriOI GRGCYC 3 cut(s) 570, 669, 699
Fsp4HI GCNGC 6 cut(s) 6, 238, 497, 1922, 1938, 2182
GluI GCNGC 6 cut(s) 6, 238, 497, 1922, 1938, 2182
GsaI CCCAGC 1 cut(s) 2566
GsuI CTGGAG 1 cut(s) 163
HaeIII GGCC 3 cut(s) 447, 1411, 1907
HapII CCGG 4 cut(s) 11, 53, 465, 803
HincII GTYRAC 1 cut(s) 1712
HindII GTYRAC 1 cut(s) 1712
HindIII AAGCTT 2 cut(s) 290, 1330
HpaII CCGG 4 cut(s) 11, 53, 465, 803
HphI GGTGA 6 cut(s) 1508, 1691, 1796, 2538, 2699, 2717
Hpy166II GTNNAC 5 cut(s) 24, 281, 1547, 1712, 2546
Hpy8I GTNNAC 5 cut(s) 24, 281, 1547, 1712, 2546
HpyAV CCTTC 7 cut(s) 604, 831, 1927, 2036, 2038, 2293, 2623
HpyCH4III ACNGT 2 cut(s) 1768, 2234
HpyCH4IV ACGT 2 cut(s) 1432, 1708
HpyF10VI GCNNNNNNNGC 5 cut(s) 174, 465, 1304, 1373, 2639
HpySE526I ACGT 2 cut(s) 1432, 1708
Ksp22I TGATCA 4 cut(s) 187, 1360, 1465, 2164
LguI GCTCTTC 1 cut(s) 1974
LmnI GCTCC 5 cut(s) 182, 403, 672, 1761, 2246
Lsp1109I GCAGC 2 cut(s) 1908, 2193
LweI GCATC 8 cut(s) 97, 736, 857, 1187, 1489, 1689, 2200, 2597
MabI ACCWGGT 1 cut(s) 211
MaeII ACGT 2 cut(s) 1432, 1708
MaeIII GTNAC 6 cut(s) 81, 955, 1186, 1339, 1585, 1611
MbiI CCGCTC 1 cut(s) 2249
MfeI CAATTG 3 cut(s) 1535, 1616, 2634
MflI RGATCY 9 cut(s) 350, 814, 1093, 1489, 1602, 1719, 2056, 2325, 2556
MhlI GDGCHC 7 cut(s) 179, 283, 475, 570, 669, 699, 1766
MlsI TGGCCA 1 cut(s) 1907
MluNI TGGCCA 1 cut(s) 1907
MlyI GAGTC 6 cut(s) 884, 1009, 1163, 1568, 1748, 2203
MmeI TCCRAC 5 cut(s) 664, 916, 1691, 1839, 2328
Mox20I TGGCCA 1 cut(s) 1907
MroXI GAANNNNTTC 3 cut(s) 1876, 1956, 2410
MscI TGGCCA 1 cut(s) 1907
Msp20I TGGCCA 1 cut(s) 1907
MspCI CTTAAG 1 cut(s) 950
MspI CCGG 4 cut(s) 11, 53, 465, 803
MspR9I CCNGG 4 cut(s) 11, 12, 213, 1542
MunI CAATTG 3 cut(s) 1535, 1616, 2634
Mva1269I GAATGC 1 cut(s) 1282
MvaI CCWGG 2 cut(s) 213, 1542
MwoI GCNNNNNNNGC 5 cut(s) 174, 465, 1304, 1373, 2639
NciI CCSGG 2 cut(s) 11, 12
NcoI CCATGG 1 cut(s) 880
NdeI CATATG 2 cut(s) 1369, 1947
NlaIV GGNNCC 5 cut(s) 51, 432, 446, 668, 1033
NmuCI GTSAC 2 cut(s) 955, 1611
PaeR7I CTCGAG 1 cut(s) 74
PagI TCATGA 2 cut(s) 763, 1018
PciSI GCTCTTC 1 cut(s) 1974
PcsI WCGNNNNNNNCGW 1 cut(s) 1807
PctI GAATGC 1 cut(s) 1282
PdmI GAANNNNTTC 3 cut(s) 1876, 1956, 2410
PfeI GAWTC 8 cut(s) 529, 716, 971, 1043, 1379, 2102, 2257, 2393
PflFI GACNNNGTC 1 cut(s) 19
PkrI GCNGC 6 cut(s) 7, 239, 498, 1923, 1939, 2183
PleI GAGTC 6 cut(s) 883, 1009, 1162, 1568, 1747, 2202
PpsI GAGTC 6 cut(s) 883, 1009, 1162, 1568, 1747, 2202
PpuMI RGGWCCY 1 cut(s) 430
PshBI ATTAAT 1 cut(s) 345
PsiI TTATAA 1 cut(s) 1506
Psp124BI GAGCTC 1 cut(s) 699
Psp5II RGGWCCY 1 cut(s) 430
Psp6I CCWGG 2 cut(s) 211, 1540
PspFI CCCAGC 1 cut(s) 2562
PspGI CCWGG 2 cut(s) 211, 1540
PspN4I GGNNCC 5 cut(s) 51, 432, 446, 668, 1033
PspPI GGNCC 4 cut(s) 50, 430, 445, 1031
PspPPI RGGWCCY 1 cut(s) 430
PstI CTGCAG 2 cut(s) 1186, 2454
PstNI CAGNNNCTG 2 cut(s) 968, 1382
PsuI RGATCY 9 cut(s) 350, 814, 1093, 1489, 1602, 1719, 2056, 2325, 2556
PsyI GACNNNGTC 1 cut(s) 19
SacI GAGCTC 1 cut(s) 699
SapI GCTCTTC 1 cut(s) 1974
SatI GCNGC 6 cut(s) 6, 238, 497, 1922, 1938, 2182
Sau96I GGNCC 4 cut(s) 50, 430, 445, 1031
ScaI AGTACT 2 cut(s) 438, 1086
SchI GAGTC 6 cut(s) 884, 1009, 1163, 1568, 1748, 2203
ScrFI CCNGG 4 cut(s) 11, 12, 213, 1542
SduI GDGCHC 7 cut(s) 179, 283, 475, 570, 669, 699, 1766
SexAI ACCWGGT 1 cut(s) 211
SfaNI GCATC 8 cut(s) 97, 736, 857, 1187, 1489, 1689, 2200, 2597
SfcI CTRYAG 3 cut(s) 1173, 1182, 2450
Sfr274I CTCGAG 1 cut(s) 74
SinI GGWCC 3 cut(s) 50, 430, 1031
SlaI CTCGAG 1 cut(s) 74
SmaI CCCGGG 1 cut(s) 12
SmlI CTYRAG 7 cut(s) 74, 563, 950, 1053, 1080, 1271, 2472
SmoI CTYRAG 7 cut(s) 74, 563, 950, 1053, 1080, 1271, 2472
SsiI CCGC 6 cut(s) 237, 497, 1553, 1937, 2249, 2732
SspI AATATT 1 cut(s) 2482
SstI GAGCTC 1 cut(s) 699
StyD4I CCNGG 4 cut(s) 9, 10, 211, 1540
StyI CCWWGG 4 cut(s) 44, 880, 1035, 1159
TaaI ACNGT 2 cut(s) 1768, 2234
TaiI ACGT 2 cut(s) 1435, 1711
TaqI TCGA 3 cut(s) 75, 1630, 1801
TatI WGTACW 7 cut(s) 436, 1084, 1166, 1546, 1848, 2050, 2225
TauI GCSGC 3 cut(s) 240, 499, 1940
TfiI GAWTC 8 cut(s) 529, 716, 971, 1043, 1379, 2102, 2257, 2393
TscAI CASTG 9 cut(s) 718, 831, 1468, 1771, 1777, 2013, 2269, 2301, 2703
TseFI GTSAC 2 cut(s) 955, 1611
TseI GCWGC 3 cut(s) 5, 1921, 2181
Tsp45I GTSAC 2 cut(s) 955, 1611
TspGWI ACGGA 3 cut(s) 2222, 2405, 2755
TspMI CCCGGG 1 cut(s) 10
TspRI CASTG 9 cut(s) 718, 831, 1468, 1771, 1777, 2013, 2269, 2301, 2703
Tth111I GACNNNGTC 1 cut(s) 19
Vha464I CTTAAG 1 cut(s) 950
VneI GTGCAC 1 cut(s) 279
VpaK11BI GGWCC 3 cut(s) 50, 430, 1031
VspI ATTAAT 1 cut(s) 345
XapI RAATTY 5 cut(s) 501, 1388, 2075, 2455, 2651
XbaI TCTAGA 1 cut(s) 2059
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XhoI CTCGAG 1 cut(s) 74
XmaI CCCGGG 1 cut(s) 10
XmaJI CCTAGG 1 cut(s) 1159
XmiI GTMKAC 1 cut(s) 23
XmnI GAANNNNTTC 3 cut(s) 1876, 1956, 2410
ZrmI AGTACT 2 cut(s) 438, 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.