Rw3G002160

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0007026
Physical Location & Seq
Reverse (-)
1918693 .. 1923280
4588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G002160.1

Sequence Viewer

Length: 2655 bp
ATGGCTGCCCCGGGGACTATGTCTACTAATCATCACAAAGTTCCTTGGGGTCCAGCATTGGTGTCAGCTAGTCTCGAGTGGTTACTGATTTTTATGCTGGTTGTGGATGCCATATTCTCATATGTAATTACGAAGTTTGCTCATTACTGGGGATTGCAAACACCATGCCTCCTGTGCTCCAGACTTGATCATGTCTTGGGCAATGAAAAACCAGGTTATTATTGGGACTTGATCTGCGGCAGTCATAAGTCAGAGATTTCTTCCTTAGTTCTTTGTCGTGCACACAACAAGCTTGTAGATGTTAATGGAATGTGTGAAAGTTGCCTATTCTCTTATGCAACCATTAATAGATCCAATGCTGAGACATACAGGTTATTGGTGGGCAAGTTGGGGGAAGCTCCTAATTCTAAGGCTGACCATGAGATTGAGGACCCAGTACTTGGGGGCCAATATGTTTGCTTTTCCGGCAGAGCACATTGTTCTTGTTGCAATGAGCCACAAATTTCTGAGGGGTATTCTCATAAGTTGATTCACTCTAAATTGTTTGGGTCTGAGGCTGAACTTGAGCCCCTATCTGGTGCTATTAGACAAAACCCTGAAGAACTGAGGAAGGCACAAGATAAATCATCTAAGTCATTGAGAGCTTCTCATTTGAGAGATACTGGGCTCCCTTTATCTCATGTTGGATACACAGAGCTCAAAGGTTCTGACACTGAATCAGAAGTTCATTTTTCTGACGGGGAGGATGCAAGTGCTTCAATTCATGAAAAGGGAAAAGGTGTTGAACCTCGTACTATTACTCCGGTTCTGATAGATCCACCTTCAGTGTCCAAGACTTTAGTTTCAGCATCAGAGATGACATTAGATAATAGGGAGTCCCATGGTTCTCTGACTCATGAAATGGATGGTCCCAAGGAAGATTCCCCTGCTCAAGCTGTTGAGCCTCATGTTATTCCTCAAGTACTAACAGATCCACCGTCTGTGTCCAAGCCCTCACTTTTGGTATCACAGGCACAAATAGATCGTATAGAGTCCCTAGGTAGTACATCTGTAGCATCTGCAGTTACTATTGGTCATGGATTGGAGGAACTTAACTTGAAAGAAGCTAGAAGAACACCTGATTATCATGCCTTATCTGAACCACTACTTGAGAATGCCTCTCCATCATCAAAGGCAATGGAAGCCCCAGTTGAAGTATCAAAGAGAAGCTTAGATGTTACAGGAACTCGTGAAATTGATCAAGCATATGTAGCAGAATCTGGGGAATTTCACAAGAGAGCTGTCAGGCCACTTAAAACATCTGAAGTACGTTTAGAAACAAACGCAGTTTCAAGCAACACTGATCAGCAAGTGGTTAATGTTTTAGATCTCGGTGATGCTTATAAGATCGTTGTTGGTAGTCAAGGAAGACAATTGCCAGGTGTACTTGCGGAACAATGGATTGGAAAGGACTCTTCAAGAGTTACTGAAGATTTGAAGATCCTGTTGTCACAATTGTCTGCCAATCGAGGGATTGAGCAATCAACAAATGAAATTATCAGTCCAACAAATAGTGGTGATCTGAAAACTATGGGGATGCAGATACGTCAACAGAGGATCTCACTAGAAAGAAATGAGTCTGGGTTGTCTATTGATGGGAGCACTGTCAGTGAAATTGAGGGTGAGAGTATGCTTGATCGATTAAAACGACAGGTTGAGCATGACAAGAAACTTTTGAGTTCTTTGTACAAGGAGTTGGAGGAAGAAAGAAATGCTTCTGCGATTTCTTCAGATCAAGCAATGGCCATGATTACAAGGCTGCAAGAGGAAAAGGCGGCACTTCATATGGAAGCACTTCAGTCCCTAAGAATGATGGAAGAGCAAGCTGAGTATGATAATGAAGCACTGCATAAGATAGATGACCTTCTTGCTGAAAAGGAGAAGGAAGTACAAGATCTAGAAGCAGAGCTAGAATTTTACAGGATTAAGTACCCAAATGAATCAATGCTAGAGTGTCTGGGAGAGACAACTTCTGATATGCAGGCTAGGGATATTGTGGTTGATCATTCAGTACCTAGCAGCCTTGCAGAGAGTCATAAAAATACGGATGCTGGAAAGCCTTGTACAGACAGTGAAGATGGGGGAGTGGGGATGATTCTCAGTGATGAGAAAACTGGTAGTTTAAAGAACTCACTGAAGGATTTTGAGGTAGAGAAGAAACAGATCTTGCAATGCTTAGAGAAGTTGGAGAAGACACTTTCTTTGTTTTCTAGTAATGGGGTAAAGACGGATTCATCTAAAGGAAATTGTTCTGAAAACGGAGGAGATGGGGCTGGCAAGTCAAATCTGCAGAATTCCGAACAGCACTCTCAAGAAAATATTAAGAAAGAAGAAAATGATTTTTCCAAGCAACATGAAGTGCCTGCATTGTCAGGACATAGTTCACCACACGAGATCCCCCAGCCCTGTCAAGCGAATGATTTAGCATCTCTATCGGTCCTGGCTTCCAATCTGAATAAGAGATTGAAGGCACTTGAGGCAGTCCTAGAATTTCTTGAACAAGCAATTACCTCTCTCAAATATGGTGAGGAGGCACTGAAGTTCATTCAGGAGATCGCTTCTCACCTAGCGGAACTACGGAAGATTGGGATTAGAAAGGATCAGACTTCAGCTTGA

Protein Analysis

884

Amino Acids

97.02

Weight (kDa)

5.14

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 560 - 650 9.9e-29 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1383
AccB7I CCANNNNNTGG 1 cut(s) 440
AccI GTMKAC 1 cut(s) 23
AciI CCGC 4 cut(s) 237, 1430, 1814, 2609
AclWI GGATC 7 cut(s) 345, 809, 965, 1474, 1604, 2428, 2646
AcoI YGGCCR 1 cut(s) 1782
AcsI RAATTY 5 cut(s) 501, 1265, 1952, 2332, 2528
AcuI CTGAAG 9 cut(s) 618, 807, 1323, 1488, 1752, 1820, 2195, 2597, 2631
AfiI CCNNNNNNNGG 3 cut(s) 440, 575, 1509
AgsI TTSAA 9 cut(s) 759, 785, 1099, 1193, 1332, 1458, 1477, 2506, 2537
AjnI CCWGG 3 cut(s) 211, 1417, 2478
AjuI GAANNNNNNNTTGG 2 cut(s) 1425, 1457
Alw21I GWGCWC 5 cut(s) 179, 283, 475, 699, 1643
Alw26I GTCTC 3 cut(s) 77, 356, 1997
Alw44I GTGCAC 1 cut(s) 279
AlwI GGATC 7 cut(s) 345, 809, 965, 1474, 1604, 2428, 2646
AlwNI CAGNNNCTG 1 cut(s) 1259
Ama87I CYCGRG 2 cut(s) 10, 74
AoxI GGCC 3 cut(s) 445, 1286, 1782
ApaLI GTGCAC 1 cut(s) 279
ApeKI GCWGC 3 cut(s) 5, 1798, 2058
ApoI RAATTY 5 cut(s) 501, 1265, 1952, 2332, 2528
ArsI GACNNNNNNTTYG 2 cut(s) 2224, 2256
AseI ATTAAT 1 cut(s) 345
Asp700I GAANNNNTTC 3 cut(s) 1753, 1833, 2287
AspA2I CCTAGG 1 cut(s) 1036
AspS9I GGNCC 5 cut(s) 50, 430, 445, 908, 2476
AsuC2I CCSGG 2 cut(s) 11, 12
AsuHPI GGTGA 6 cut(s) 1385, 1568, 1673, 2415, 2576, 2594
AvaI CYCGRG 2 cut(s) 10, 74
AvaII GGWCC 4 cut(s) 50, 430, 908, 2476
AvrII CCTAGG 1 cut(s) 1036
BaeGI GKGCMC 1 cut(s) 283
BalI TGGCCA 1 cut(s) 1784
BanII GRGCYC 3 cut(s) 570, 669, 699
BauI CACGAG 2 cut(s) 1227, 2429
BbsI GAAGAC 2 cut(s) 1414, 2237
Bbv12I GWGCWC 5 cut(s) 179, 283, 475, 699, 1643
BbvI GCAGC 2 cut(s) 1785, 2070
BccI CCATC 6 cut(s) 899, 1171, 1628, 1846, 2111, 2300
BcgI CGANNNNNNTGC 2 cut(s) 2454, 2488
BciT130I CCWGG 3 cut(s) 213, 1419, 2480
BciVI GTATCC 1 cut(s) 680
BclI TGATCA 4 cut(s) 187, 1237, 1342, 2041
BcnI CCSGG 2 cut(s) 11, 12
BcoDI GTCTC 3 cut(s) 77, 356, 1997
BfmI CTRYAG 3 cut(s) 1050, 1059, 2327
BfuI GTATCC 1 cut(s) 680
BglII AGATCT 3 cut(s) 1366, 1933, 2202
BisI GCNGC 5 cut(s) 6, 238, 1799, 1815, 2059
BlnI CCTAGG 1 cut(s) 1036
BlsI GCNGC 5 cut(s) 7, 239, 1800, 1816, 2060
BmcAI AGTACT 2 cut(s) 438, 963
Bme1390I CCNGG 5 cut(s) 11, 12, 213, 1419, 2480
Bme18I GGWCC 4 cut(s) 50, 430, 908, 2476
BmeT110I CYCGRG 2 cut(s) 10, 74
BmgT120I GGNCC 5 cut(s) 50, 430, 445, 908, 2476
BmiI GGNNCC 5 cut(s) 51, 432, 446, 668, 910
BmrFI CCNGG 5 cut(s) 11, 12, 213, 1419, 2480
BmrI ACTGGG 4 cut(s) 157, 428, 672, 1181
BmsI GCATC 8 cut(s) 97, 736, 857, 1064, 1366, 1566, 2077, 2474
BmuI ACTGGG 4 cut(s) 157, 428, 672, 1181
BpiI GAAGAC 2 cut(s) 1414, 2237
BplI GAGNNNNNCTC 4 cut(s) 631, 663, 1142, 1174
BpmI CTGGAG 1 cut(s) 163
BpuEI CTTGAG 6 cut(s) 584, 915, 942, 1169, 2334, 2534
BpuMI CCSGG 2 cut(s) 11, 12
Bsa29I ATCGAT 1 cut(s) 1678
BsaJI CCNNGG 7 cut(s) 9, 10, 11, 44, 880, 912, 1036
BsaWI WCCGGW 1 cut(s) 802
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 784, 814
Bsc4I CCNNNNNNNGG 3 cut(s) 440, 575, 1509
Bse1I ACTGG 5 cut(s) 152, 434, 667, 1187, 2158
Bse3DI GCAATG 5 cut(s) 208, 496, 1182, 1785, 2216
BseBI CCWGG 3 cut(s) 213, 1419, 2480
BseCI ATCGAT 1 cut(s) 1678
BseDI CCNNGG 7 cut(s) 9, 10, 11, 44, 880, 912, 1036
BseGI GGATG 6 cut(s) 112, 751, 910, 1581, 2092, 2136
BseLI CCNNNNNNNGG 3 cut(s) 440, 575, 1509
BseMI GCAATG 5 cut(s) 208, 496, 1182, 1785, 2216
BseMII CTCAG 6 cut(s) 351, 498, 543, 596, 1857, 2152
BseNI ACTGG 5 cut(s) 152, 434, 667, 1187, 2158
BseRI GAGGAG 2 cut(s) 2316, 2582
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 2 cut(s) 1785, 2070
BseYI CCCAGC 1 cut(s) 2439
BshFI GGCC 3 cut(s) 447, 1288, 1784
BshVI ATCGAT 1 cut(s) 1678
BsiHKAI GWGCWC 5 cut(s) 179, 283, 475, 699, 1643
BsiHKCI CYCGRG 2 cut(s) 10, 74
BsiSI CCGG 3 cut(s) 11, 465, 803
BslFI GGGAC 6 cut(s) 28, 239, 862, 894, 1018, 1825
BslI CCNNNNNNNGG 3 cut(s) 440, 575, 1509
BsmAI GTCTC 3 cut(s) 77, 356, 1997
BsmFI GGGAC 6 cut(s) 28, 239, 862, 894, 1018, 1825
BsmI GAATGC 1 cut(s) 1159
BsnI GGCC 3 cut(s) 447, 1288, 1784
BsoBI CYCGRG 2 cut(s) 10, 74
Bsp1286I GDGCHC 7 cut(s) 179, 283, 475, 570, 669, 699, 1643
Bsp1407I TGTACA 2 cut(s) 1725, 2102
Bsp19I CCATGG 1 cut(s) 880
BspACI CCGC 4 cut(s) 237, 1430, 1814, 2609
BspANI GGCC 3 cut(s) 447, 1288, 1784
BspCNI CTCAG 6 cut(s) 352, 499, 544, 597, 1858, 2151
BspDI ATCGAT 1 cut(s) 1678
BspHI TCATGA 2 cut(s) 763, 895
BspLI GGNNCC 5 cut(s) 51, 432, 446, 668, 910
BspMAI CTGCAG 2 cut(s) 1063, 2331
BspPI GGATC 7 cut(s) 345, 809, 965, 1474, 1604, 2428, 2646
BspQI GCTCTTC 1 cut(s) 1851
BsrDI GCAATG 5 cut(s) 208, 496, 1182, 1785, 2216
BsrGI TGTACA 2 cut(s) 1725, 2102
BsrI ACTGG 5 cut(s) 152, 434, 667, 1187, 2158
BssECI CCNNGG 7 cut(s) 9, 10, 11, 44, 880, 912, 1036
BssSI CACGAG 2 cut(s) 1227, 2429
BssT1I CCWWGG 4 cut(s) 44, 880, 912, 1036
Bst2BI CACGAG 2 cut(s) 1227, 2429
Bst2UI CCWGG 3 cut(s) 213, 1419, 2480
Bst4CI ACNGT 3 cut(s) 978, 1645, 2111
Bst6I CTCTTC 2 cut(s) 1459, 1851
BstAUI TGTACA 2 cut(s) 1725, 2102
BstC8I GCNNGC 4 cut(s) 1863, 2022, 2314, 2403
BstDSI CCRYGG 1 cut(s) 880
BstF5I GGATG 6 cut(s) 112, 751, 910, 1581, 2092, 2136
BstMAI GTCTC 3 cut(s) 77, 356, 1997
BstMWI GCNNNNNNNGC 5 cut(s) 174, 465, 1181, 1250, 2516
BstNI CCWGG 3 cut(s) 213, 1419, 2480
BstSCI CCNGG 5 cut(s) 9, 10, 211, 1417, 2478
BstSFI CTRYAG 3 cut(s) 1050, 1059, 2327
BstSLI GKGCMC 1 cut(s) 283
BstV1I GCAGC 2 cut(s) 1785, 2070
BstV2I GAAGAC 2 cut(s) 1414, 2237
BstX2I RGATCY 9 cut(s) 350, 814, 970, 1366, 1479, 1596, 1933, 2202, 2433
BstYI RGATCY 9 cut(s) 350, 814, 970, 1366, 1479, 1596, 1933, 2202, 2433
Bsu15I ATCGAT 1 cut(s) 1678
BsuI GTATCC 1 cut(s) 680
BsuRI GGCC 3 cut(s) 447, 1288, 1784
BsuTUI ATCGAT 1 cut(s) 1678
BtgI CCRYGG 1 cut(s) 880
BtsCI GGATG 6 cut(s) 112, 751, 910, 1581, 2092, 2136
BtsI GCAGTG 1 cut(s) 1883
Cac8I GCNNGC 4 cut(s) 1863, 2022, 2314, 2403
CaiI CAGNNNCTG 1 cut(s) 1259
CciI TCATGA 2 cut(s) 763, 895
Cfr13I GGNCC 5 cut(s) 50, 430, 445, 908, 2476
Cfr9I CCCGGG 1 cut(s) 10
ClaI ATCGAT 1 cut(s) 1678
CsiI ACCWGGT 1 cut(s) 211
DraI TTTAAA 1 cut(s) 2163
EaeI YGGCCR 1 cut(s) 1782
Eam1104I CTCTTC 2 cut(s) 1459, 1851
EarI CTCTTC 2 cut(s) 1459, 1851
Ecl136II GAGCTC 1 cut(s) 697
Eco130I CCWWGG 4 cut(s) 44, 880, 912, 1036
Eco24I GRGCYC 3 cut(s) 570, 669, 699
Eco47I GGWCC 4 cut(s) 50, 430, 908, 2476
Eco53kI GAGCTC 1 cut(s) 697
Eco57I CTGAAG 9 cut(s) 618, 807, 1323, 1488, 1752, 1820, 2195, 2597, 2631
Eco88I CYCGRG 2 cut(s) 10, 74
EcoICRI GAGCTC 1 cut(s) 697
EcoO109I RGGNCCY 1 cut(s) 430
EcoRI GAATTC 1 cut(s) 2332
EcoRII CCWGG 3 cut(s) 211, 1417, 2478
EcoT14I CCWWGG 4 cut(s) 44, 880, 912, 1036
EcoT38I GRGCYC 3 cut(s) 570, 669, 699
ErhI CCWWGG 4 cut(s) 44, 880, 912, 1036
FalI AAGNNNNNCTT 4 cut(s) 1193, 1225, 1738, 1770
FaqI GGGAC 6 cut(s) 28, 239, 862, 894, 1018, 1825
FauNDI CATATG 3 cut(s) 121, 1246, 1824
FbaI TGATCA 4 cut(s) 187, 1237, 1342, 2041
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 5 cut(s) 6, 238, 1799, 1815, 2059
FokI GGATG 6 cut(s) 119, 758, 917, 1588, 2099, 2143
FriOI GRGCYC 3 cut(s) 570, 669, 699
Fsp4HI GCNGC 5 cut(s) 6, 238, 1799, 1815, 2059
GluI GCNGC 5 cut(s) 6, 238, 1799, 1815, 2059
GsaI CCCAGC 1 cut(s) 2443
GsuI CTGGAG 1 cut(s) 163
HaeIII GGCC 3 cut(s) 447, 1288, 1784
HapII CCGG 3 cut(s) 11, 465, 803
HincII GTYRAC 1 cut(s) 1589
HindII GTYRAC 1 cut(s) 1589
HindIII AAGCTT 2 cut(s) 290, 1207
HpaII CCGG 3 cut(s) 11, 465, 803
HphI GGTGA 6 cut(s) 1385, 1568, 1673, 2415, 2576, 2594
Hpy166II GTNNAC 5 cut(s) 24, 281, 1424, 1589, 2423
Hpy8I GTNNAC 5 cut(s) 24, 281, 1424, 1589, 2423
HpyAV CCTTC 6 cut(s) 604, 831, 1913, 1915, 2170, 2500
HpyCH4III ACNGT 3 cut(s) 978, 1645, 2111
HpyCH4IV ACGT 2 cut(s) 1309, 1585
HpyF10VI GCNNNNNNNGC 5 cut(s) 174, 465, 1181, 1250, 2516
HpySE526I ACGT 2 cut(s) 1309, 1585
Ksp22I TGATCA 4 cut(s) 187, 1237, 1342, 2041
LguI GCTCTTC 1 cut(s) 1851
LmnI GCTCC 4 cut(s) 182, 403, 672, 1638
Lsp1109I GCAGC 2 cut(s) 1785, 2070
LweI GCATC 8 cut(s) 97, 736, 857, 1064, 1366, 1566, 2077, 2474
MabI ACCWGGT 1 cut(s) 211
MaeII ACGT 2 cut(s) 1309, 1585
MaeIII GTNAC 5 cut(s) 81, 1063, 1216, 1462, 1488
MfeI CAATTG 2 cut(s) 1412, 1493
MflI RGATCY 9 cut(s) 350, 814, 970, 1366, 1479, 1596, 1933, 2202, 2433
MhlI GDGCHC 7 cut(s) 179, 283, 475, 570, 669, 699, 1643
MlsI TGGCCA 1 cut(s) 1784
MluNI TGGCCA 1 cut(s) 1784
MlyI GAGTC 6 cut(s) 884, 886, 1040, 1445, 1625, 2080
MmeI TCCRAC 4 cut(s) 664, 1568, 1716, 2205
Mox20I TGGCCA 1 cut(s) 1784
MroXI GAANNNNTTC 3 cut(s) 1753, 1833, 2287
MscI TGGCCA 1 cut(s) 1784
MseI TTAA 9 cut(s) 303, 345, 1092, 1293, 1356, 1682, 1965, 2162, 2361
Msp20I TGGCCA 1 cut(s) 1784
MspI CCGG 3 cut(s) 11, 465, 803
MspR9I CCNGG 5 cut(s) 11, 12, 213, 1419, 2480
MunI CAATTG 2 cut(s) 1412, 1493
Mva1269I GAATGC 1 cut(s) 1159
MvaI CCWGG 3 cut(s) 213, 1419, 2480
MwoI GCNNNNNNNGC 5 cut(s) 174, 465, 1181, 1250, 2516
NciI CCSGG 2 cut(s) 11, 12
NcoI CCATGG 1 cut(s) 880
NdeI CATATG 3 cut(s) 121, 1246, 1824
NlaIV GGNNCC 5 cut(s) 51, 432, 446, 668, 910
NmuCI GTSAC 1 cut(s) 1488
PaeR7I CTCGAG 1 cut(s) 74
PagI TCATGA 2 cut(s) 763, 895
PciSI GCTCTTC 1 cut(s) 1851
PcsI WCGNNNNNNNCGW 1 cut(s) 1684
PctI GAATGC 1 cut(s) 1159
PdmI GAANNNNTTC 3 cut(s) 1753, 1833, 2287
PfeI GAWTC 7 cut(s) 529, 716, 920, 1256, 1979, 2134, 2270
PflFI GACNNNGTC 1 cut(s) 19
PflMI CCANNNNNTGG 1 cut(s) 440
PkrI GCNGC 5 cut(s) 7, 239, 1800, 1816, 2060
PleI GAGTC 6 cut(s) 883, 886, 1039, 1445, 1624, 2079
PpsI GAGTC 6 cut(s) 883, 886, 1039, 1445, 1624, 2079
PpuMI RGGWCCY 1 cut(s) 430
PshBI ATTAAT 1 cut(s) 345
PsiI TTATAA 1 cut(s) 1383
Psp124BI GAGCTC 1 cut(s) 699
Psp5II RGGWCCY 1 cut(s) 430
Psp6I CCWGG 3 cut(s) 211, 1417, 2478
PspFI CCCAGC 1 cut(s) 2439
PspGI CCWGG 3 cut(s) 211, 1417, 2478
PspN4I GGNNCC 5 cut(s) 51, 432, 446, 668, 910
PspPI GGNCC 5 cut(s) 50, 430, 445, 908, 2476
PspPPI RGGWCCY 1 cut(s) 430
PstI CTGCAG 2 cut(s) 1063, 2331
PstNI CAGNNNCTG 1 cut(s) 1259
PsuI RGATCY 9 cut(s) 350, 814, 970, 1366, 1479, 1596, 1933, 2202, 2433
PsyI GACNNNGTC 1 cut(s) 19
SacI GAGCTC 1 cut(s) 699
SapI GCTCTTC 1 cut(s) 1851
SaqAI TTAA 9 cut(s) 303, 345, 1092, 1293, 1356, 1682, 1965, 2162, 2361
SatI GCNGC 5 cut(s) 6, 238, 1799, 1815, 2059
Sau96I GGNCC 5 cut(s) 50, 430, 445, 908, 2476
ScaI AGTACT 2 cut(s) 438, 963
SchI GAGTC 6 cut(s) 884, 886, 1040, 1445, 1625, 2080
ScrFI CCNGG 5 cut(s) 11, 12, 213, 1419, 2480
SduI GDGCHC 7 cut(s) 179, 283, 475, 570, 669, 699, 1643
SexAI ACCWGGT 1 cut(s) 211
SfaNI GCATC 8 cut(s) 97, 736, 857, 1064, 1366, 1566, 2077, 2474
SfcI CTRYAG 3 cut(s) 1050, 1059, 2327
Sfr274I CTCGAG 1 cut(s) 74
SinI GGWCC 4 cut(s) 50, 430, 908, 2476
SlaI CTCGAG 1 cut(s) 74
SmaI CCCGGG 1 cut(s) 12
SmlI CTYRAG 7 cut(s) 74, 563, 930, 957, 1148, 2349, 2513
SmoI CTYRAG 7 cut(s) 74, 563, 930, 957, 1148, 2349, 2513
SsiI CCGC 4 cut(s) 237, 1430, 1814, 2609
SspI AATATT 1 cut(s) 2359
SstI GAGCTC 1 cut(s) 699
StyD4I CCNGG 5 cut(s) 9, 10, 211, 1417, 2478
StyI CCWWGG 4 cut(s) 44, 880, 912, 1036
TaaI ACNGT 3 cut(s) 978, 1645, 2111
TaiI ACGT 2 cut(s) 1312, 1588
TaqI TCGA 3 cut(s) 75, 1507, 1678
TaqII GACCGA 1 cut(s) 2464
TatI WGTACW 7 cut(s) 436, 961, 1043, 1423, 1725, 1927, 2102
TauI GCSGC 2 cut(s) 240, 1817
TfiI GAWTC 7 cut(s) 529, 716, 920, 1256, 1979, 2134, 2270
Tru1I TTAA 9 cut(s) 303, 345, 1092, 1293, 1356, 1682, 1965, 2162, 2361
Tru9I TTAA 9 cut(s) 303, 345, 1092, 1293, 1356, 1682, 1965, 2162, 2361
TseFI GTSAC 1 cut(s) 1488
TseI GCWGC 3 cut(s) 5, 1798, 2058
Tsp45I GTSAC 1 cut(s) 1488
TspGWI ACGGA 4 cut(s) 2099, 2282, 2313, 2632
TspMI CCCGGG 1 cut(s) 10
Tth111I GACNNNGTC 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 440
VneI GTGCAC 1 cut(s) 279
VpaK11BI GGWCC 4 cut(s) 50, 430, 908, 2476
VspI ATTAAT 1 cut(s) 345
XapI RAATTY 5 cut(s) 501, 1265, 1952, 2332, 2528
XbaI TCTAGA 1 cut(s) 1936
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XhoI CTCGAG 1 cut(s) 74
XmaI CCCGGG 1 cut(s) 10
XmaJI CCTAGG 1 cut(s) 1036
XmiI GTMKAC 1 cut(s) 23
XmnI GAANNNNTTC 3 cut(s) 1753, 1833, 2287
ZrmI AGTACT 2 cut(s) 438, 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.