Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006168

Physical Location & Seq

Genomic Coordinates Reverse (-)

31189961 .. 31194483

Gene Structure 4523 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1232700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGCGCAGACTCTTCCCTCCAAACCCAATCAAAACTCCAACTCTCTTCAACTCCCTCCGTCTGCGATTGAACTCGCTCCGATCCCACTGTGCCAACTCAATGGCCTTACCTCCGCCGAAGTCAGCCATGGCTTCCGCCGTCGTCGACAACGAGGTGGGTCTCGCCCGCCGCTTCTGGATCAAGTTCAACCGAGAATCGATTTTCGCTATGTACACTCCCTTCTCGCTCTGTTTGGCTTCTGGGAATCTCAAGATTGAAACTTTCCGCAATTATATTGCCCAAGATGTTCACTTTCTCAAGGCCTTCGCTCACGCGTATGAATTGGCAGAAGATTGTGCAGACGATGATGATGCAAAGCCTGTGATTTCTGAGTTGAGGAAGGCAGTTCGGCAGGAGCTGAAAATGCATGATTCATTTGTGAAGGGATGGGGTTTACAGGGTGCTAAAGAGGCCCCTATCAACTCTGCTGCCGTGAAGTACACAGATTTCTTATTGGCAACAGCATCTGGAAAAGTTGAAGGAGTCAAGGGTCCTGGTAAACTTGCAACTCCATTTGAAAGAACCAAAGTTGCTGCTTACACCCTTGGCGCTATGACTCCTTGCATGAGACTGTATGCCTTTCTTGGTAAAGAGTTCAAGGCCCTTCTAGATCCCAACGAAGGCAGTCACCCGTACTTGAAGTGGATTGACAATTATTCTTCTGAAAGTTTTCAGGCATCAGCTGTACAAACCGAAGAGTTGCTCGATAAACTAAGTGTCTCTTTGACAGGCGAGGAACTTGACATAATCGAAAAGCTTTATCACCAAGCAATGAAACTTGAAATAGAGTTCTTCTCTGCTCAGCCCCTTGTTCAGCCAACTGTAGTTCCTCTGATCAAAGAGCATAACCCTGCAGAAGATCGGCTCGTGATATTTTCTGATTTTGACTTGACTTGTACGATCGTCGATTCATCTGCCATTTTGGCTGAAATTGCAATAGTAACAGCACCAAAATCTGATCAAAAATCTGATCAACATCAGCCCGAAAATCATATTGCTCGGATGTCATCAGCTGATCTCAGGAATACATGGGGTCTTCTTTCCAGGCAGTACACAGAAGAGTATGAGCAATGCATAGAAAGCATAGTTCCCACTGAAAAAGCGGTGTTTGACTATGAAAAGTTGCATAAAGCACTAGAGAAACTTTCAGATTTTGAGAGGAAGGCAAACAATAGAGTTACAAAGTCAGGAGTACTCAAGGGTCTTAATCTCGAAGATATAAAAAGAGCTGGTGAACGTCTCATTCTTCAAGATGGTTGTATTAATTTCTTCCAGAAAATTGTCAAGAGTGAAAACTTGAATACAAATGTTCATGTTCTTTCGTACTGTTGGTGTGGTGATCTCATAAGGTCGGCCTTTTCATCAGGTGGTTTACCCGAGCTGAATGTGCATGCAAATGAGTTTACTTTTAAGGAATCCATATCCACAGGTGACATTGTTAAGAAGGTGGAGTCTCCTATTAACAAGGTTCAATCTTTCAAAGAAATATTGAAAAATTGCAGCAATGACAGAAAGAACTTGACCGTTTACATTGGAGACTCAGTTGGTGATTTACTCTGTCTGCTGGAGGCAGATATTGGTATCGTAATTGGGTCAAGTTCAAGCCTTAGGAGAGTGGCGACTCAGTTTGGGGTATCTTTTGTTCCTTTGTTTGCGGGTTTAGTTAAGAAACAGAAAGAATGCACAGATGGAAGGTCTTCTAATTGGAAAGGGTTAACCGGTATTCTTTACACAGTGAATAGTTGGGCGGAAATACATGCCTTCATTTTGGGTTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009110 GO:0009228 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0017144 GO:0018130 GO:0019438 GO:0034641 GO:0042131 GO:0042364 GO:0042578 GO:0042723 GO:0042724 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0072527 GO:0072528 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TENA_THI-4

Protein Analysis

606

Amino Acids

67.47

Weight (kDa)

6.53

Isoelectric Point (pI)

40.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TENA_THI-4	PF03070	73 - 170	1.9e-08	TENA/THI-4/PQQC family
TENA_THI-4	PF03070	190 - 279	1.1e-07	TENA/THI-4/PQQC family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006168)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G32470
fragaria_vesca	FvH4_6g01520 FvH4_6g01520 FvH4_6g01520 FvH4_6g01520 FvH4_6g01520 FvH4_6g01520
malus_domestica	MD04G1232700.v1.1 MD12G1250300.v1.1
prunus_persica	Prupe.6G352500_v2.0.a1 Prupe.6G352500_v2.0.a1 Prupe.6G352500_v2.0.a1 Prupe.6G352500_v2.0.a1 Prupe.6G352500_v2.0.a1
pyrus_communis	pycom04g20600 pycom12g23040
rosa_chinensis	RchiOBHm_Chr3g0449211
rosa_laevigata	RLG00000025845
rosa_multiflora	Rmu_sc0004874.1_g000007
rosa_roxburghii	Rroxscaffold_6G00427250
rosa_rugosa	Rorug02G0618800 Rorug02G0618900
rosa_samantha	Rh3AG017100 Rh3BG017200 Rh3CG016200 Rh3DG017500
rosa_wichuraiana	Rw3G001300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	5
AccI	GTMKAC	1 cut(s)	144
AccII	CGCG	1 cut(s)	314
AciI	CCGC	8 cut(s)	113, 135, 166, 169, 265, 1142, 1694, 1787
AclWI	GGATC	3 cut(s)	75, 185, 644
AfaI	GTAC	8 cut(s)	212, 479, 674, 726, 937, 1091, 1233, 1364
AfiI	CCNNNNNNNGG	1 cut(s)	659
AflIII	ACRYGT	1 cut(s)	312
AgeI	ACCGGT	1 cut(s)	1757
AjnI	CCWGG	2 cut(s)	532, 1082
AluBI	AGCT	6 cut(s)	397, 722, 796, 1052, 1268, 1420
AluI	AGCT	6 cut(s)	397, 722, 796, 1052, 1268, 1420
Alw26I	GTCTC	6 cut(s)	164, 601, 763, 1283, 1497, 1569
AlwI	GGATC	3 cut(s)	75, 185, 644
AlwNI	CAGNNNCTG	2 cut(s)	397, 506
Ama87I	CYCGRG	1 cut(s)	1415
AoxI	GGCC	5 cut(s)	102, 300, 450, 639, 1392
ApeKI	GCWGC	3 cut(s)	467, 572, 1539
AseI	ATTAAT	1 cut(s)	1302
AsiGI	ACCGGT	1 cut(s)	1757
Asp700I	GAANNNNTTC	2 cut(s)	708, 1735
AspLEI	GCGC	2 cut(s)	6, 590
AspS9I	GGNCC	3 cut(s)	451, 530, 640
AsuHPI	GGTGA	6 cut(s)	659, 794, 1283, 1388, 1481, 1598
AvaI	CYCGRG	1 cut(s)	1415
AvaII	GGWCC	1 cut(s)	530
AxyI	CCTNAGG	1 cut(s)	1646
BarI	GAAGNNNNNNTAC	2 cut(s)	203, 235
BauI	CACGAG	1 cut(s)	905
BbsI	GAAGAC	2 cut(s)	1067, 1728
BbvI	GCAGC	3 cut(s)	454, 559, 1551
BccI	CCATC	3 cut(s)	420, 1286, 1721
BceAI	ACGGC	2 cut(s)	122, 455
BcgI	CGANNNNNNTGC	4 cut(s)	332, 366, 935, 969
BciT130I	CCWGG	2 cut(s)	534, 1084
BclI	TGATCA	3 cut(s)	873, 997, 1009
BcoDI	GTCTC	6 cut(s)	164, 601, 763, 1283, 1497, 1569
BfaI	CTAG	2 cut(s)	647, 1175
BfmI	CTRYAG	2 cut(s)	861, 891
BfoI	RGCGCY	1 cut(s)	591
BglI	GCCNNNNNGGC	1 cut(s)	962
BisI	GCNGC	4 cut(s)	169, 468, 573, 1540
BlpI	GCTNAGC	1 cut(s)	840
BlsI	GCNGC	4 cut(s)	170, 469, 574, 1541
BmcAI	AGTACT	1 cut(s)	1233
Bme1390I	CCNGG	2 cut(s)	534, 1084
Bme18I	GGWCC	1 cut(s)	530
BmeT110I	CYCGRG	1 cut(s)	1415
BmgT120I	GGNCC	3 cut(s)	451, 530, 640
BmiI	GGNNCC	2 cut(s)	453, 531
BmrFI	CCNGG	2 cut(s)	534, 1084
BmsI	GCATC	3 cut(s)	340, 512, 725
BpiI	GAAGAC	2 cut(s)	1067, 1728
BplI	GAGNNNNNCTC	2 cut(s)	818, 850
BpmI	CTGGAG	1 cut(s)	1625
Bpu1102I	GCTNAGC	1 cut(s)	840
BpuEI	CTTGAG	3 cut(s)	233, 281, 1220
Bsa29I	ATCGAT	1 cut(s)	197
BsaBI	GATNNNNATC	1 cut(s)	1014
BsaI	GGTCTC	1 cut(s)	164
BsaJI	CCNNGG	2 cut(s)	126, 583
BsaWI	WCCGGW	1 cut(s)	1757
BsaXI	ACNNNNNCTCC	2 cut(s)	1566, 1596
Bsc4I	CCNNNNNNNGG	1 cut(s)	659
Bse118I	RCCGGY	1 cut(s)	1757
Bse21I	CCTNAGG	1 cut(s)	1646
Bse3DI	GCAATG	3 cut(s)	816, 1115, 1549
Bse8I	GATNNNNATC	1 cut(s)	1014
BseBI	CCWGG	2 cut(s)	534, 1084
BseCI	ATCGAT	1 cut(s)	197
BseDI	CCNNGG	2 cut(s)	126, 583
BseGI	GGATG	2 cut(s)	431, 1047
BseJI	GATNNNNATC	1 cut(s)	1014
BseLI	CCNNNNNNNGG	1 cut(s)	659
BseMI	GCAATG	3 cut(s)	816, 1115, 1549
BseMII	CTCAG	5 cut(s)	360, 854, 1072, 1593, 1676
BseXI	GCAGC	3 cut(s)	454, 559, 1551
BsgI	GTGCAG	1 cut(s)	357
Bsh1236I	CGCG	1 cut(s)	314
Bsh1285I	CGRYCG	1 cut(s)	942
BshFI	GGCC	5 cut(s)	104, 302, 452, 641, 1394
BshTI	ACCGGT	1 cut(s)	1757
BshVI	ATCGAT	1 cut(s)	197
BsiEI	CGRYCG	1 cut(s)	942
BsiHKCI	CYCGRG	1 cut(s)	1415
BsiSI	CCGG	1 cut(s)	1758
BslI	CCNNNNNNNGG	1 cut(s)	659
BsmAI	GTCTC	6 cut(s)	164, 601, 763, 1283, 1497, 1569
BsmBI	CGTCTC	1 cut(s)	1283
BsmI	GAATGC	1 cut(s)	1724
BsnI	GGCC	5 cut(s)	104, 302, 452, 641, 1394
Bso31I	GGTCTC	1 cut(s)	164
BsoBI	CYCGRG	1 cut(s)	1415
Bsp1407I	TGTACA	2 cut(s)	210, 724
Bsp1720I	GCTNAGC	1 cut(s)	840
Bsp19I	CCATGG	1 cut(s)	126
BspACI	CCGC	8 cut(s)	113, 135, 166, 169, 265, 1142, 1694, 1787
BspANI	GGCC	5 cut(s)	104, 302, 452, 641, 1394
BspCNI	CTCAG	5 cut(s)	361, 853, 1071, 1592, 1675
BspDI	ATCGAT	1 cut(s)	197
BspFNI	CGCG	1 cut(s)	314
BspLI	GGNNCC	2 cut(s)	453, 531
BspMAI	CTGCAG	1 cut(s)	895
BspPI	GGATC	3 cut(s)	75, 185, 644
BspTNI	GGTCTC	1 cut(s)	164
BsrDI	GCAATG	3 cut(s)	816, 1115, 1549
BsrFI	RCCGGY	1 cut(s)	1757
BsrGI	TGTACA	2 cut(s)	210, 724
BssAI	RCCGGY	1 cut(s)	1757
BssECI	CCNNGG	2 cut(s)	126, 583
BssSI	CACGAG	1 cut(s)	905
BssT1I	CCWWGG	2 cut(s)	126, 583
Bst2BI	CACGAG	1 cut(s)	905
Bst2UI	CCWGG	2 cut(s)	534, 1084
Bst4CI	ACNGT	6 cut(s)	89, 612, 862, 1367, 1564, 1774
Bst6I	CTCTTC	4 cut(s)	17, 50, 729, 1092
BstAUI	TGTACA	2 cut(s)	210, 724
BstC8I	GCNNGC	2 cut(s)	166, 1431
BstDEI	CTNAG	7 cut(s)	369, 752, 840, 1058, 1579, 1646, 1662
BstDSI	CCRYGG	1 cut(s)	126
BstF5I	GGATG	2 cut(s)	431, 1047
BstFNI	CGCG	1 cut(s)	314
BstH2I	RGCGCY	1 cut(s)	591
BstHHI	GCGC	2 cut(s)	6, 590
BstMAI	GTCTC	6 cut(s)	164, 601, 763, 1283, 1497, 1569
BstMCI	CGRYCG	1 cut(s)	942
BstMWI	GCNNNNNNNGC	6 cut(s)	403, 449, 962, 971, 1119, 1426
BstNI	CCWGG	2 cut(s)	534, 1084
BstNSI	RCATGY	2 cut(s)	1433, 1799
BstSCI	CCNGG	2 cut(s)	532, 1082
BstSFI	CTRYAG	2 cut(s)	861, 891
BstUI	CGCG	1 cut(s)	314
BstV1I	GCAGC	3 cut(s)	454, 559, 1551
BstV2I	GAAGAC	2 cut(s)	1067, 1728
BstX2I	RGATCY	1 cut(s)	649
BstXI	CCANNNNNNTGG	1 cut(s)	100
BstYI	RGATCY	1 cut(s)	649
Bsu15I	ATCGAT	1 cut(s)	197
Bsu36I	CCTNAGG	1 cut(s)	1646
BsuRI	GGCC	5 cut(s)	104, 302, 452, 641, 1394
BsuTUI	ATCGAT	1 cut(s)	197
BtgI	CCRYGG	1 cut(s)	126
BtsCI	GGATG	2 cut(s)	431, 1047
BtsIMutI	CAGTG	3 cut(s)	85, 1131, 1779
Cac8I	GCNNGC	2 cut(s)	166, 1431
CaiI	CAGNNNCTG	2 cut(s)	397, 506
CfoI	GCGC	2 cut(s)	6, 590
Cfr10I	RCCGGY	1 cut(s)	1757
Cfr13I	GGNCC	3 cut(s)	451, 530, 640
ClaI	ATCGAT	1 cut(s)	197
Csp6I	GTAC	8 cut(s)	211, 478, 673, 725, 936, 1090, 1232, 1363
CspAI	ACCGGT	1 cut(s)	1757
CviAII	CATG	7 cut(s)	127, 407, 604, 1068, 1352, 1430, 1796
CviQI	GTAC	8 cut(s)	211, 478, 673, 725, 936, 1090, 1232, 1363
DdeI	CTNAG	7 cut(s)	369, 752, 840, 1058, 1579, 1646, 1662
Eam1104I	CTCTTC	4 cut(s)	17, 50, 729, 1092
EarI	CTCTTC	4 cut(s)	17, 50, 729, 1092
EciI	GGCGGA	3 cut(s)	102, 124, 1802
Eco130I	CCWWGG	2 cut(s)	126, 583
Eco147I	AGGCCT	1 cut(s)	302
Eco31I	GGTCTC	1 cut(s)	164
Eco47I	GGWCC	1 cut(s)	530
Eco81I	CCTNAGG	1 cut(s)	1646
Eco88I	CYCGRG	1 cut(s)	1415
EcoO109I	RGGNCCY	3 cut(s)	451, 530, 640
EcoRII	CCWGG	2 cut(s)	532, 1082
EcoT14I	CCWWGG	2 cut(s)	126, 583
EcoT22I	ATGCAT	2 cut(s)	408, 1115
ErhI	CCWWGG	2 cut(s)	126, 583
Esp3I	CGTCTC	1 cut(s)	1283
FaeI	CATG	7 cut(s)	130, 410, 607, 1071, 1355, 1433, 1799
FalI	AAGNNNNNCTT	2 cut(s)	745, 777
FatI	CATG	7 cut(s)	126, 406, 603, 1067, 1351, 1429, 1795
FauI	CCCGC	2 cut(s)	173, 1687
FbaI	TGATCA	3 cut(s)	873, 997, 1009
FblI	GTMKAC	1 cut(s)	144
Fnu4HI	GCNGC	4 cut(s)	169, 468, 573, 1540
FokI	GGATG	2 cut(s)	438, 1054
Fsp4HI	GCNGC	4 cut(s)	169, 468, 573, 1540
FspBI	CTAG	2 cut(s)	647, 1175
FspI	TGCGCA	1 cut(s)	5
GlaI	GCGC	2 cut(s)	5, 589
GluI	GCNGC	4 cut(s)	169, 468, 573, 1540
GsuI	CTGGAG	1 cut(s)	1625
HaeII	RGCGCY	1 cut(s)	591
HaeIII	GGCC	5 cut(s)	104, 302, 452, 641, 1394
HapII	CCGG	1 cut(s)	1758
HhaI	GCGC	2 cut(s)	6, 590
Hin1II	CATG	7 cut(s)	130, 410, 607, 1071, 1355, 1433, 1799
Hin6I	GCGC	2 cut(s)	4, 588
HinP1I	GCGC	2 cut(s)	4, 588
HincII	GTYRAC	2 cut(s)	145, 1755
HindII	GTYRAC	2 cut(s)	145, 1755
HindIII	AAGCTT	1 cut(s)	794
HpaI	GTTAAC	1 cut(s)	1755
HpaII	CCGG	1 cut(s)	1758
HphI	GGTGA	6 cut(s)	659, 794, 1283, 1388, 1481, 1598
Hpy188I	TCNGA	9 cut(s)	80, 370, 703, 873, 919, 997, 1009, 1041, 1189
Hpy99I	CGWCG	3 cut(s)	143, 146, 947
HpyCH4III	ACNGT	6 cut(s)	89, 612, 862, 1367, 1564, 1774
HpyCH4IV	ACGT	1 cut(s)	1276
HpyF10VI	GCNNNNNNNGC	6 cut(s)	403, 449, 962, 971, 1119, 1426
HpyF3I	CTNAG	7 cut(s)	369, 752, 840, 1058, 1579, 1646, 1662
HpySE526I	ACGT	1 cut(s)	1276
Hsp92II	CATG	7 cut(s)	130, 410, 607, 1071, 1355, 1433, 1799
HspAI	GCGC	2 cut(s)	4, 588
Ksp22I	TGATCA	3 cut(s)	873, 997, 1009
KspAI	GTTAAC	1 cut(s)	1755
LmnI	GCTCC	2 cut(s)	81, 394
Lsp1109I	GCAGC	3 cut(s)	454, 559, 1551
LweI	GCATC	3 cut(s)	340, 512, 725
MaeI	CTAG	2 cut(s)	647, 1175
MaeII	ACGT	1 cut(s)	1276
MaeIII	GTNAC	4 cut(s)	665, 979, 1216, 1469
MflI	RGATCY	1 cut(s)	649
MluCI	AATT	9 cut(s)	268, 320, 691, 969, 1303, 1317, 1534, 1626, 1741
MluI	ACGCGT	1 cut(s)	312
MlyI	GAGTC	6 cut(s)	3, 531, 589, 1499, 1571, 1654
MmeI	TCCRAC	1 cut(s)	62
Mph1103I	ATGCAT	2 cut(s)	408, 1115
MroXI	GAANNNNTTC	2 cut(s)	708, 1735
MseI	TTAA	7 cut(s)	1245, 1302, 1449, 1479, 1500, 1704, 1754
MslI	CAYNNNNRTG	2 cut(s)	315, 1434
MspA1I	CMGCKG	2 cut(s)	722, 1052
MspI	CCGG	1 cut(s)	1758
MspR9I	CCNGG	2 cut(s)	534, 1084
Mva1269I	GAATGC	1 cut(s)	1724
MvaI	CCWGG	2 cut(s)	534, 1084
MvnI	CGCG	1 cut(s)	314
MwoI	GCNNNNNNNGC	6 cut(s)	403, 449, 962, 971, 1119, 1426
NcoI	CCATGG	1 cut(s)	126
NlaIII	CATG	7 cut(s)	130, 410, 607, 1071, 1355, 1433, 1799
NlaIV	GGNNCC	2 cut(s)	453, 531
NmuCI	GTSAC	2 cut(s)	665, 1469
NsbI	TGCGCA	1 cut(s)	5
NsiI	ATGCAT	2 cut(s)	408, 1115
NspI	RCATGY	2 cut(s)	1433, 1799
PaeI	GCATGC	1 cut(s)	1433
PceI	AGGCCT	1 cut(s)	302
PcsI	WCGNNNNNNNCGW	1 cut(s)	147
PctI	GAATGC	1 cut(s)	1724
PdmI	GAANNNNTTC	2 cut(s)	708, 1735
PfeI	GAWTC	5 cut(s)	194, 244, 410, 947, 1454
PinAI	ACCGGT	1 cut(s)	1757
PkrI	GCNGC	4 cut(s)	170, 469, 574, 1541
Ple19I	CGATCG	1 cut(s)	942
PleI	GAGTC	6 cut(s)	3, 530, 589, 1498, 1571, 1654
PpsI	GAGTC	6 cut(s)	3, 530, 589, 1498, 1571, 1654
PpuMI	RGGWCCY	1 cut(s)	530
PshBI	ATTAAT	1 cut(s)	1302
Psp5II	RGGWCCY	1 cut(s)	530
Psp6I	CCWGG	2 cut(s)	532, 1082
PspGI	CCWGG	2 cut(s)	532, 1082
PspN4I	GGNNCC	2 cut(s)	453, 531
PspPI	GGNCC	3 cut(s)	451, 530, 640
PspPPI	RGGWCCY	1 cut(s)	530
PsrI	GAACNNNNNNTAC	2 cut(s)	1665, 1697
PstI	CTGCAG	1 cut(s)	895
PstNI	CAGNNNCTG	2 cut(s)	397, 506
PsuI	RGATCY	1 cut(s)	649
PvuI	CGATCG	1 cut(s)	942
PvuII	CAGCTG	2 cut(s)	722, 1052
RsaI	GTAC	8 cut(s)	212, 479, 674, 726, 937, 1091, 1233, 1364
RsaNI	GTAC	8 cut(s)	211, 478, 673, 725, 936, 1090, 1232, 1363
RseI	CAYNNNNRTG	2 cut(s)	315, 1434
SalI	GTCGAC	1 cut(s)	143
SaqAI	TTAA	7 cut(s)	1245, 1302, 1449, 1479, 1500, 1704, 1754
SatI	GCNGC	4 cut(s)	169, 468, 573, 1540
Sau96I	GGNCC	3 cut(s)	451, 530, 640
ScaI	AGTACT	1 cut(s)	1233
SchI	GAGTC	6 cut(s)	3, 531, 589, 1499, 1571, 1654
ScrFI	CCNGG	2 cut(s)	534, 1084
SfaNI	GCATC	3 cut(s)	340, 512, 725
SfcI	CTRYAG	2 cut(s)	861, 891
SinI	GGWCC	1 cut(s)	530
SmiMI	CAYNNNNRTG	2 cut(s)	315, 1434
SmlI	CTYRAG	3 cut(s)	248, 296, 1235
SmoI	CTYRAG	3 cut(s)	248, 296, 1235
SphI	GCATGC	1 cut(s)	1433
Sse9I	AATT	9 cut(s)	268, 320, 691, 969, 1303, 1317, 1534, 1626, 1741
SseBI	AGGCCT	1 cut(s)	302
SsiI	CCGC	8 cut(s)	113, 135, 166, 169, 265, 1142, 1694, 1787
SspI	AATATT	1 cut(s)	1527
SspMI	CTAG	2 cut(s)	647, 1175
StuI	AGGCCT	1 cut(s)	302
StyD4I	CCNGG	2 cut(s)	532, 1082
StyI	CCWWGG	2 cut(s)	126, 583
TaaI	ACNGT	6 cut(s)	89, 612, 862, 1367, 1564, 1774
TaiI	ACGT	1 cut(s)	1279
TaqI	TCGA	6 cut(s)	144, 197, 744, 789, 945, 1251
TasI	AATT	9 cut(s)	268, 320, 691, 969, 1303, 1317, 1534, 1626, 1741
TatI	WGTACW	5 cut(s)	210, 477, 724, 1089, 1231
TauI	GCSGC	1 cut(s)	171
TfiI	GAWTC	5 cut(s)	194, 244, 410, 947, 1454
Tru1I	TTAA	7 cut(s)	1245, 1302, 1449, 1479, 1500, 1704, 1754
Tru9I	TTAA	7 cut(s)	1245, 1302, 1449, 1479, 1500, 1704, 1754
TscAI	CASTG	3 cut(s)	92, 1138, 1779
TseFI	GTSAC	2 cut(s)	665, 1469
TseI	GCWGC	3 cut(s)	467, 572, 1539
Tsp45I	GTSAC	2 cut(s)	665, 1469
TspDTI	ATGAA	8 cut(s)	333, 402, 827, 939, 1170, 1340, 1389, 1792
TspGWI	ACGGA	1 cut(s)	47
TspRI	CASTG	3 cut(s)	92, 1138, 1779
VpaK11BI	GGWCC	1 cut(s)	530
VspI	ATTAAT	1 cut(s)	1302
XbaI	TCTAGA	1 cut(s)	646
XceI	RCATGY	2 cut(s)	1433, 1799
XmiI	GTMKAC	1 cut(s)	144
XmnI	GAANNNNTTC	2 cut(s)	708, 1735
XspI	CTAG	2 cut(s)	647, 1175
ZrmI	AGTACT	1 cut(s)	1233
Zsp2I	ATGCAT	2 cut(s)	408, 1115

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.