Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006168

Physical Location & Seq

Genomic Coordinates Reverse (-)

32053839 .. 32058813

Gene Structure 4975 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1250300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1806 bp

ATGCGCATACTCTTCCCCCCAAACCCAATCAAAACCCCAACTCTCTTCAACTTCCTCCGTCTGCGATTCAACTCGCTCCGATCCCACTGTGCCAACTCAATGGCCGTACCTCCGCCGAAGTCAGCCATGGCTTCCGCCGTCGTCGACAACGAGGTGGGTCTCGCCCGCCGCTTCTGGATCAAGTTCAAGCGAGAATCGATTTTCGCTAAGTACACTCCCTTCACGCTCTGTTTGGCTGCTGGGAATCTAAAGATTGAAACTTTCCGCGATTATATTGCCCAAGATGTTCACTTTCTCAAGGCCTTCGCTCAAGCGTATGAATTGGCAGAAGATTGTGCAGACGATGATGATGCAAAGCCTGTGATTTCTGAGTTGAGGAGGGCAGTTCTGCAGGAGCTGAAAATGCATGATTCATTTGTGAAGGAATGGGGTTTACAGGGTGCTGAAGAGACCCCTATCAACTCCGCTGCAGTGAAGTACACAGATTTCTTATTGGCAACAGCCTCTGGAAAAGTTGAAGGAGTCAAGGGACCTGGTAAACTTGCAACTCCATTTGAAAGAACCAAAGTGGCTGCTTACACCCTTGGCGCTATGACTCCTTGCATGAGGCTGTATGCCTTTCTTGGTAAGGAGTTCCAGGCACTTCTAGATCCCAGCAAAGGCAGTCACCCGTACTTGAAGTGGATTGACAGTTATTCTTCTGAAAGTTTTCAGGCATCAGCTGTGCAAATCGAAGAGTTGCTCGATAAACTAAGTGTCTCTTTGACAGGCGAGGAGCTTGACATCATCGAAAATCTTTATCACCAAGCAATGAAACTTGAGATCGAGTTCTTCTCTGCTCAGTCTCTTGTTCAGCCAACTGCAGTTCCTCTGATCAGAGAACATAACCCTGCAGAAGATCGGCTCATGATATTTTCTGATTTCGATTTGACTTGTACAGTCGTTGATTCATCTGCCATTTTGGCTGAAATTGCAATAGTAACAGCACCAAAATCTGATCAACATCAACCCGAAAATCATCTTGCTCGGATGTCTTCGGCTGATCTCAGGAATACATGGGGTCTTCTTTCCAGGCAGTACACAGAAGAGTATGAGCAATGCATAGAAAGCATTGTTCCCACTGAAAAAGCCGTGTTTGACTATGAAAATTTGCTAAAAGCACTAGAGAAACTTTCAGATTTTGAGAGGAAGGCAAACAATAGAGTCACGAAGTCTGAAGTACTCAAGGGTCTAAATCTCGAAGATATAAAAAGAGCTGGTGAACGTCTCATTCTTCAAGATGGTTGTATTAATTTCTTCCAGAAAATTGCCAAGAGTGAAAACATGAATGCAAATGTTCATGTTCTTTCATACTGTTGGTGTGGTGATCTCATAAGATCGGCCTTTTCATCAGGGGGTTTAAACGAGCTGGATGTACATGCAAATGAGTTTACCTTCGAGGAATCCATCTCCACAGGTGATATTGTTAAGAAGGTGGAGTCCCCTATTGACAAGGTTAAATCTTTTAAAGATATTTTGAAAAATTGCAGCAATGACAGAAAGAACTTGACTGTTTACATTGGAGACTCAGTGGGTGACTTACTTTGTCTGCTGGAGGCGGATATTGGAATCGTAATTGGGTCAAGTTCAAGCCTTAGGAGAGTGGCGACTCAGTTTGGGGTATCTTTTGTTCCGTTGTTCCCGGGTTTAGTTAAGAAACAGAAAGAATGCACAGATGGAAGGTCTCCTAGTTGGAAAGGGTTAACCGGTATTCTTTACACAGTGAATAGTTGGGCGGAAATACATGCCTTCATTTTGGGGTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009110 GO:0009228 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0017144 GO:0018130 GO:0019438 GO:0034641 GO:0042131 GO:0042364 GO:0042578 GO:0042723 GO:0042724 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0072527 GO:0072528 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TENA_THI-4

Protein Analysis

602

Amino Acids

67.1

Weight (kDa)

5.57

Isoelectric Point (pI)

42.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TENA_THI-4	PF03070	73 - 170	2.9e-09	TENA/THI-4/PQQC family
TENA_THI-4	PF03070	190 - 280	1.5e-06	TENA/THI-4/PQQC family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006168)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G32470
fragaria_vesca	FvH4_6g01520 FvH4_6g01520 FvH4_6g01520 FvH4_6g01520 FvH4_6g01520 FvH4_6g01520
malus_domestica	MD04G1232700.v1.1 MD12G1250300.v1.1
prunus_persica	Prupe.6G352500_v2.0.a1 Prupe.6G352500_v2.0.a1 Prupe.6G352500_v2.0.a1 Prupe.6G352500_v2.0.a1 Prupe.6G352500_v2.0.a1
pyrus_communis	pycom04g20600 pycom12g23040
rosa_chinensis	RchiOBHm_Chr3g0449211
rosa_laevigata	RLG00000025845
rosa_multiflora	Rmu_sc0004874.1_g000007
rosa_roxburghii	Rroxscaffold_6G00427250
rosa_rugosa	Rorug02G0618800 Rorug02G0618900
rosa_samantha	Rh3AG017100 Rh3BG017200 Rh3CG016200 Rh3DG017500
rosa_wichuraiana	Rw3G001300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	5
AccI	GTMKAC	1 cut(s)	144
AccII	CGCG	1 cut(s)	267
AciI	CCGC	8 cut(s)	113, 135, 166, 169, 265, 465, 1598, 1775
AclWI	GGATC	3 cut(s)	75, 185, 644
AcoI	YGGCCR	1 cut(s)	102
AcsI	RAATTY	1 cut(s)	1147
AcuI	CTGAAG	2 cut(s)	465, 1236
AfaI	GTAC	8 cut(s)	108, 212, 479, 674, 937, 1079, 1221, 1416
AfiI	CCNNNNNNNGG	1 cut(s)	659
AgeI	ACCGGT	1 cut(s)	1745
AjnI	CCWGG	3 cut(s)	532, 636, 1070
AluBI	AGCT	5 cut(s)	397, 722, 778, 1256, 1408
AluI	AGCT	5 cut(s)	397, 722, 778, 1256, 1408
Alw26I	GTCTC	7 cut(s)	164, 443, 763, 849, 1271, 1557, 1728
AlwI	GGATC	3 cut(s)	75, 185, 644
AlwNI	CAGNNNCTG	2 cut(s)	397, 506
Ama87I	CYCGRG	1 cut(s)	1681
AoxI	GGCC	3 cut(s)	102, 300, 1380
ApeKI	GCWGC	4 cut(s)	236, 467, 572, 1527
ApoI	RAATTY	1 cut(s)	1147
AseI	ATTAAT	1 cut(s)	1290
AsiGI	ACCGGT	1 cut(s)	1745
Asp700I	GAANNNNTTC	1 cut(s)	708
AspLEI	GCGC	2 cut(s)	6, 590
AspS9I	GGNCC	1 cut(s)	530
AsuC2I	CCSGG	2 cut(s)	1682, 1683
AsuHPI	GGTGA	6 cut(s)	659, 794, 1271, 1376, 1469, 1586
AvaI	CYCGRG	1 cut(s)	1681
AvaII	GGWCC	1 cut(s)	530
AxyI	CCTNAGG	1 cut(s)	1634
BarI	GAAGNNNNNNTAC	2 cut(s)	203, 235
BbsI	GAAGAC	2 cut(s)	1026, 1055
BbvI	GCAGC	4 cut(s)	223, 454, 559, 1539
BccI	CCATC	3 cut(s)	1274, 1454, 1709
BceAI	ACGGC	3 cut(s)	89, 122, 1115
BcgI	CGANNNNNNTGC	4 cut(s)	257, 291, 332, 366
BciT130I	CCWGG	3 cut(s)	534, 638, 1072
BclI	TGATCA	2 cut(s)	873, 997
BcnI	CCSGG	2 cut(s)	1682, 1683
BcoDI	GTCTC	7 cut(s)	164, 443, 763, 849, 1271, 1557, 1728
BfaI	CTAG	3 cut(s)	647, 1163, 1728
BfmI	CTRYAG	4 cut(s)	389, 468, 861, 891
BfoI	RGCGCY	1 cut(s)	591
BglI	GCCNNNNNGGC	1 cut(s)	962
BisI	GCNGC	5 cut(s)	169, 237, 468, 573, 1528
BlsI	GCNGC	5 cut(s)	170, 238, 469, 574, 1529
BmcAI	AGTACT	1 cut(s)	1221
Bme1390I	CCNGG	5 cut(s)	534, 638, 1072, 1682, 1683
Bme18I	GGWCC	1 cut(s)	530
BmeT110I	CYCGRG	1 cut(s)	1681
BmgT120I	GGNCC	1 cut(s)	530
BmiI	GGNNCC	1 cut(s)	531
BmrFI	CCNGG	5 cut(s)	534, 638, 1072, 1682, 1683
BmsI	GCATC	2 cut(s)	340, 725
BpiI	GAAGAC	2 cut(s)	1026, 1055
BplI	GAGNNNNNCTC	2 cut(s)	818, 850
BpmI	CTGGAG	1 cut(s)	1613
BpuEI	CTTGAG	4 cut(s)	281, 294, 839, 1208
BpuMI	CCSGG	2 cut(s)	1682, 1683
Bsa29I	ATCGAT	1 cut(s)	197
BsaBI	GATNNNNATC	1 cut(s)	1002
BsaI	GGTCTC	3 cut(s)	164, 443, 1728
BsaJI	CCNNGG	3 cut(s)	126, 583, 1681
BsaWI	WCCGGW	1 cut(s)	1745
BsaXI	ACNNNNNCTCC	2 cut(s)	1554, 1584
Bsc4I	CCNNNNNNNGG	1 cut(s)	659
Bse118I	RCCGGY	1 cut(s)	1745
Bse21I	CCTNAGG	1 cut(s)	1634
Bse3DI	GCAATG	3 cut(s)	816, 1103, 1537
Bse8I	GATNNNNATC	1 cut(s)	1002
BseBI	CCWGG	3 cut(s)	534, 638, 1072
BseCI	ATCGAT	1 cut(s)	197
BseDI	CCNNGG	3 cut(s)	126, 583, 1681
BseGI	GGATG	2 cut(s)	1035, 1417
BseJI	GATNNNNATC	1 cut(s)	1002
BseLI	CCNNNNNNNGG	1 cut(s)	659
BseMI	GCAATG	3 cut(s)	816, 1103, 1537
BseMII	CTCAG	5 cut(s)	360, 854, 1060, 1581, 1664
BseRI	GAGGAG	2 cut(s)	391, 788
BseXI	GCAGC	4 cut(s)	223, 454, 559, 1539
BseYI	CCCAGC	2 cut(s)	239, 653
BsgI	GTGCAG	1 cut(s)	357
Bsh1236I	CGCG	1 cut(s)	267
BshFI	GGCC	3 cut(s)	104, 302, 1382
BshTI	ACCGGT	1 cut(s)	1745
BshVI	ATCGAT	1 cut(s)	197
BsiHKCI	CYCGRG	1 cut(s)	1681
BsiSI	CCGG	2 cut(s)	1682, 1746
BslFI	GGGAC	2 cut(s)	543, 1465
BslI	CCNNNNNNNGG	1 cut(s)	659
BsmAI	GTCTC	7 cut(s)	164, 443, 763, 849, 1271, 1557, 1728
BsmBI	CGTCTC	1 cut(s)	1271
BsmFI	GGGAC	2 cut(s)	543, 1465
BsmI	GAATGC	2 cut(s)	1333, 1712
BsnI	GGCC	3 cut(s)	104, 302, 1382
Bso31I	GGTCTC	3 cut(s)	164, 443, 1728
BsoBI	CYCGRG	1 cut(s)	1681
Bsp1407I	TGTACA	2 cut(s)	935, 1414
Bsp19I	CCATGG	1 cut(s)	126
BspACI	CCGC	8 cut(s)	113, 135, 166, 169, 265, 465, 1598, 1775
BspANI	GGCC	3 cut(s)	104, 302, 1382
BspCNI	CTCAG	5 cut(s)	361, 853, 1059, 1580, 1663
BspDI	ATCGAT	1 cut(s)	197
BspFNI	CGCG	1 cut(s)	267
BspHI	TCATGA	1 cut(s)	906
BspLI	GGNNCC	1 cut(s)	531
BspMAI	CTGCAG	4 cut(s)	393, 472, 865, 895
BspPI	GGATC	3 cut(s)	75, 185, 644
BspTNI	GGTCTC	3 cut(s)	164, 443, 1728
BsrDI	GCAATG	3 cut(s)	816, 1103, 1537
BsrFI	RCCGGY	1 cut(s)	1745
BsrGI	TGTACA	2 cut(s)	935, 1414
BssAI	RCCGGY	1 cut(s)	1745
BssECI	CCNNGG	3 cut(s)	126, 583, 1681
BssT1I	CCWWGG	2 cut(s)	126, 583
Bst2UI	CCWGG	3 cut(s)	534, 638, 1072
Bst4CI	ACNGT	6 cut(s)	89, 692, 940, 1355, 1552, 1762
Bst6I	CTCTTC	5 cut(s)	17, 50, 441, 729, 1080
BstAUI	TGTACA	2 cut(s)	935, 1414
BstC8I	GCNNGC	1 cut(s)	166
BstDEI	CTNAG	8 cut(s)	207, 369, 752, 840, 1046, 1567, 1634, 1650
BstDSI	CCRYGG	1 cut(s)	126
BstF5I	GGATG	2 cut(s)	1035, 1417
BstFNI	CGCG	1 cut(s)	267
BstH2I	RGCGCY	1 cut(s)	591
BstHHI	GCGC	2 cut(s)	6, 590
BstMAI	GTCTC	7 cut(s)	164, 443, 763, 849, 1271, 1557, 1728
BstMWI	GCNNNNNNNGC	4 cut(s)	403, 962, 971, 1107
BstNI	CCWGG	3 cut(s)	534, 638, 1072
BstNSI	RCATGY	2 cut(s)	1421, 1787
BstSCI	CCNGG	5 cut(s)	532, 636, 1070, 1680, 1681
BstSFI	CTRYAG	4 cut(s)	389, 468, 861, 891
BstUI	CGCG	1 cut(s)	267
BstV1I	GCAGC	4 cut(s)	223, 454, 559, 1539
BstV2I	GAAGAC	2 cut(s)	1026, 1055
BstX2I	RGATCY	1 cut(s)	649
BstXI	CCANNNNNNTGG	1 cut(s)	100
BstYI	RGATCY	1 cut(s)	649
Bsu15I	ATCGAT	1 cut(s)	197
Bsu36I	CCTNAGG	1 cut(s)	1634
BsuRI	GGCC	3 cut(s)	104, 302, 1382
BsuTUI	ATCGAT	1 cut(s)	197
BtgI	CCRYGG	1 cut(s)	126
BtsCI	GGATG	2 cut(s)	1035, 1417
BtsI	GCAGTG	1 cut(s)	477
BtsIMutI	CAGTG	5 cut(s)	85, 477, 1119, 1575, 1767
Cac8I	GCNNGC	1 cut(s)	166
CaiI	CAGNNNCTG	2 cut(s)	397, 506
CciI	TCATGA	1 cut(s)	906
CfoI	GCGC	2 cut(s)	6, 590
Cfr10I	RCCGGY	1 cut(s)	1745
Cfr13I	GGNCC	1 cut(s)	530
Cfr9I	CCCGGG	1 cut(s)	1681
ClaI	ATCGAT	1 cut(s)	197
CsiI	ACCWGGT	1 cut(s)	532
Csp6I	GTAC	8 cut(s)	107, 211, 478, 673, 936, 1078, 1220, 1415
CspAI	ACCGGT	1 cut(s)	1745
CviAII	CATG	9 cut(s)	127, 407, 604, 907, 1056, 1324, 1340, 1418, 1784
CviQI	GTAC	8 cut(s)	107, 211, 478, 673, 936, 1078, 1220, 1415
DdeI	CTNAG	8 cut(s)	207, 369, 752, 840, 1046, 1567, 1634, 1650
DraI	TTTAAA	2 cut(s)	1401, 1507
EaeI	YGGCCR	1 cut(s)	102
Eam1104I	CTCTTC	5 cut(s)	17, 50, 441, 729, 1080
EarI	CTCTTC	5 cut(s)	17, 50, 441, 729, 1080
EciI	GGCGGA	4 cut(s)	102, 124, 1613, 1790
Eco130I	CCWWGG	2 cut(s)	126, 583
Eco147I	AGGCCT	1 cut(s)	302
Eco31I	GGTCTC	3 cut(s)	164, 443, 1728
Eco47I	GGWCC	1 cut(s)	530
Eco57I	CTGAAG	2 cut(s)	465, 1236
Eco81I	CCTNAGG	1 cut(s)	1634
Eco88I	CYCGRG	1 cut(s)	1681
EcoO109I	RGGNCCY	1 cut(s)	530
EcoRII	CCWGG	3 cut(s)	532, 636, 1070
EcoT14I	CCWWGG	2 cut(s)	126, 583
EcoT22I	ATGCAT	2 cut(s)	408, 1103
ErhI	CCWWGG	2 cut(s)	126, 583
Esp3I	CGTCTC	1 cut(s)	1271
FaeI	CATG	9 cut(s)	130, 410, 607, 910, 1059, 1327, 1343, 1421, 1787
FalI	AAGNNNNNCTT	2 cut(s)	745, 777
FaqI	GGGAC	2 cut(s)	543, 1465
FatI	CATG	9 cut(s)	126, 406, 603, 906, 1055, 1323, 1339, 1417, 1783
FauI	CCCGC	1 cut(s)	173
FbaI	TGATCA	2 cut(s)	873, 997
FblI	GTMKAC	1 cut(s)	144
Fnu4HI	GCNGC	5 cut(s)	169, 237, 468, 573, 1528
FokI	GGATG	2 cut(s)	1042, 1424
Fsp4HI	GCNGC	5 cut(s)	169, 237, 468, 573, 1528
FspAI	RTGCGCAY	1 cut(s)	5
FspBI	CTAG	3 cut(s)	647, 1163, 1728
FspI	TGCGCA	1 cut(s)	5
GlaI	GCGC	2 cut(s)	5, 589
GluI	GCNGC	5 cut(s)	169, 237, 468, 573, 1528
GsaI	CCCAGC	2 cut(s)	243, 657
GsuI	CTGGAG	1 cut(s)	1613
HaeII	RGCGCY	1 cut(s)	591
HaeIII	GGCC	3 cut(s)	104, 302, 1382
HapII	CCGG	2 cut(s)	1682, 1746
HhaI	GCGC	2 cut(s)	6, 590
Hin1II	CATG	9 cut(s)	130, 410, 607, 910, 1059, 1327, 1343, 1421, 1787
Hin6I	GCGC	2 cut(s)	4, 588
HinP1I	GCGC	2 cut(s)	4, 588
HincII	GTYRAC	2 cut(s)	145, 1743
HindII	GTYRAC	2 cut(s)	145, 1743
HpaI	GTTAAC	1 cut(s)	1743
HpaII	CCGG	2 cut(s)	1682, 1746
HphI	GGTGA	6 cut(s)	659, 794, 1271, 1376, 1469, 1586
Hpy188III	TCNNGA	9 cut(s)	175, 507, 647, 907, 1048, 1207, 1238, 1277, 1300
Hpy99I	CGWCG	2 cut(s)	143, 146
HpyAV	CCTTC	9 cut(s)	229, 313, 415, 512, 1183, 1444, 1465, 1713, 1798
HpyCH4III	ACNGT	6 cut(s)	89, 692, 940, 1355, 1552, 1762
HpyCH4IV	ACGT	1 cut(s)	1264
HpyF10VI	GCNNNNNNNGC	4 cut(s)	403, 962, 971, 1107
HpyF3I	CTNAG	8 cut(s)	207, 369, 752, 840, 1046, 1567, 1634, 1650
HpySE526I	ACGT	1 cut(s)	1264
Hsp92II	CATG	9 cut(s)	130, 410, 607, 910, 1059, 1327, 1343, 1421, 1787
HspAI	GCGC	2 cut(s)	4, 588
Ksp22I	TGATCA	2 cut(s)	873, 997
KspAI	GTTAAC	1 cut(s)	1743
LmnI	GCTCC	3 cut(s)	81, 394, 775
Lsp1109I	GCAGC	4 cut(s)	223, 454, 559, 1539
LweI	GCATC	2 cut(s)	340, 725
MabI	ACCWGGT	1 cut(s)	532
MaeI	CTAG	3 cut(s)	647, 1163, 1728
MaeII	ACGT	1 cut(s)	1264
MaeIII	GTNAC	4 cut(s)	665, 979, 1204, 1574
MflI	RGATCY	1 cut(s)	649
MluCI	AATT	7 cut(s)	320, 969, 1147, 1291, 1305, 1522, 1614
MlyI	GAGTC	6 cut(s)	531, 589, 1212, 1487, 1559, 1642
MmeI	TCCRAC	1 cut(s)	1712
Mph1103I	ATGCAT	2 cut(s)	408, 1103
MroXI	GAANNNNTTC	1 cut(s)	708
MseI	TTAA	7 cut(s)	1290, 1400, 1467, 1497, 1506, 1692, 1742
MslI	CAYNNNNRTG	1 cut(s)	1422
MspA1I	CMGCKG	2 cut(s)	467, 722
MspI	CCGG	2 cut(s)	1682, 1746
MspR9I	CCNGG	5 cut(s)	534, 638, 1072, 1682, 1683
MssI	GTTTAAAC	1 cut(s)	1401
Mva1269I	GAATGC	2 cut(s)	1333, 1712
MvaI	CCWGG	3 cut(s)	534, 638, 1072
MvnI	CGCG	1 cut(s)	267
MwoI	GCNNNNNNNGC	4 cut(s)	403, 962, 971, 1107
NciI	CCSGG	2 cut(s)	1682, 1683
NcoI	CCATGG	1 cut(s)	126
NlaIII	CATG	9 cut(s)	130, 410, 607, 910, 1059, 1327, 1343, 1421, 1787
NlaIV	GGNNCC	1 cut(s)	531
NmuCI	GTSAC	3 cut(s)	665, 1204, 1574
NsbI	TGCGCA	1 cut(s)	5
NsiI	ATGCAT	2 cut(s)	408, 1103
NspI	RCATGY	2 cut(s)	1421, 1787
PagI	TCATGA	1 cut(s)	906
PceI	AGGCCT	1 cut(s)	302
PcsI	WCGNNNNNNNCGW	1 cut(s)	147
PctI	GAATGC	2 cut(s)	1333, 1712
PdmI	GAANNNNTTC	1 cut(s)	708
PfeI	GAWTC	7 cut(s)	66, 194, 244, 410, 947, 1442, 1608
PinAI	ACCGGT	1 cut(s)	1745
PkrI	GCNGC	5 cut(s)	170, 238, 469, 574, 1529
PleI	GAGTC	6 cut(s)	530, 589, 1211, 1486, 1559, 1642
PmeI	GTTTAAAC	1 cut(s)	1401
PpsI	GAGTC	6 cut(s)	530, 589, 1211, 1486, 1559, 1642
PpuMI	RGGWCCY	1 cut(s)	530
PshBI	ATTAAT	1 cut(s)	1290
Psp5II	RGGWCCY	1 cut(s)	530
Psp6I	CCWGG	3 cut(s)	532, 636, 1070
PspFI	CCCAGC	2 cut(s)	239, 653
PspGI	CCWGG	3 cut(s)	532, 636, 1070
PspN4I	GGNNCC	1 cut(s)	531
PspPI	GGNCC	1 cut(s)	530
PspPPI	RGGWCCY	1 cut(s)	530
PsrI	GAACNNNNNNTAC	2 cut(s)	1653, 1685
PstI	CTGCAG	4 cut(s)	393, 472, 865, 895
PstNI	CAGNNNCTG	2 cut(s)	397, 506
PsuI	RGATCY	1 cut(s)	649
PvuII	CAGCTG	1 cut(s)	722
RsaI	GTAC	8 cut(s)	108, 212, 479, 674, 937, 1079, 1221, 1416
RsaNI	GTAC	8 cut(s)	107, 211, 478, 673, 936, 1078, 1220, 1415
RseI	CAYNNNNRTG	1 cut(s)	1422
SalI	GTCGAC	1 cut(s)	143
SaqAI	TTAA	7 cut(s)	1290, 1400, 1467, 1497, 1506, 1692, 1742
SatI	GCNGC	5 cut(s)	169, 237, 468, 573, 1528
Sau96I	GGNCC	1 cut(s)	530
ScaI	AGTACT	1 cut(s)	1221
SchI	GAGTC	6 cut(s)	531, 589, 1212, 1487, 1559, 1642
ScrFI	CCNGG	5 cut(s)	534, 638, 1072, 1682, 1683
SexAI	ACCWGGT	1 cut(s)	532
SfaNI	GCATC	2 cut(s)	340, 725
SfcI	CTRYAG	4 cut(s)	389, 468, 861, 891
SinI	GGWCC	1 cut(s)	530
SmaI	CCCGGG	1 cut(s)	1683
SmiMI	CAYNNNNRTG	1 cut(s)	1422
SmlI	CTYRAG	4 cut(s)	296, 309, 818, 1223
SmoI	CTYRAG	4 cut(s)	296, 309, 818, 1223
Sse9I	AATT	7 cut(s)	320, 969, 1147, 1291, 1305, 1522, 1614
SseBI	AGGCCT	1 cut(s)	302
SsiI	CCGC	8 cut(s)	113, 135, 166, 169, 265, 465, 1598, 1775
SspMI	CTAG	3 cut(s)	647, 1163, 1728
StuI	AGGCCT	1 cut(s)	302
StyD4I	CCNGG	5 cut(s)	532, 636, 1070, 1680, 1681
StyI	CCWWGG	2 cut(s)	126, 583
TaaI	ACNGT	6 cut(s)	89, 692, 940, 1355, 1552, 1762
TaiI	ACGT	1 cut(s)	1267
TaqI	TCGA	9 cut(s)	144, 197, 732, 744, 789, 825, 924, 1239, 1437
TasI	AATT	7 cut(s)	320, 969, 1147, 1291, 1305, 1522, 1614
TatI	WGTACW	6 cut(s)	210, 477, 935, 1077, 1219, 1414
TauI	GCSGC	1 cut(s)	171
TfiI	GAWTC	7 cut(s)	66, 194, 244, 410, 947, 1442, 1608
Tru1I	TTAA	7 cut(s)	1290, 1400, 1467, 1497, 1506, 1692, 1742
Tru9I	TTAA	7 cut(s)	1290, 1400, 1467, 1497, 1506, 1692, 1742
TscAI	CASTG	5 cut(s)	92, 477, 1126, 1575, 1767
TseFI	GTSAC	3 cut(s)	665, 1204, 1574
TseI	GCWGC	4 cut(s)	236, 467, 572, 1527
Tsp45I	GTSAC	3 cut(s)	665, 1204, 1574
TspGWI	ACGGA	2 cut(s)	47, 1662
TspMI	CCCGGG	1 cut(s)	1681
TspRI	CASTG	5 cut(s)	92, 477, 1126, 1575, 1767
VpaK11BI	GGWCC	1 cut(s)	530
VspI	ATTAAT	1 cut(s)	1290
XapI	RAATTY	1 cut(s)	1147
XbaI	TCTAGA	1 cut(s)	646
XceI	RCATGY	2 cut(s)	1421, 1787
XmaI	CCCGGG	1 cut(s)	1681
XmiI	GTMKAC	1 cut(s)	144
XmnI	GAANNNNTTC	1 cut(s)	708
XspI	CTAG	3 cut(s)	647, 1163, 1728
ZrmI	AGTACT	1 cut(s)	1221
Zsp2I	ATGCAT	2 cut(s)	408, 1103

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.