MD04G1241900.v1.1

Monothiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011891
Physical Location & Seq
Reverse (-)
31876529 .. 31878689
2161 bp
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UTR
Exon/CDS
Intron
MD04G1241900.v1.1.491

Sequence Viewer

Length: 549 bp
ATGTGGTGTGTTGGGGCCTTGGACTCAAAAACCGTAACTCCGTCTGCCTCTATTCCTCTTGCTGGAGCTGCGAGCAGATCCCCTACCAATTCCCTCGCTTCACTCCACTTCCACGGTGCCTCTCGGCGTCCCTGCCCTCCTTCCGCCGTCTCTCTTTCATATCACTCGTCCTTCGGACCCAAGCTCTCTTCGAAGAAGACCCTCTCATTCCGATGCTACGCCGCATTAACTCCGGAGCTGAAGAACACATTGGACAAAGTTGTTACTTCACACAAAGTGGTTCTTTTTATGAAGGGAACAAAGGATTTTCCACAATGTGGTTTTTCAAGCACTTGCGTACAGATATTGAGGTCTTTGAATGTACCGTTCGAAACAATAAACATACTAGAAAATGAACTTTTGCGCCAGGGATTGAAGGAGTACTCGAGTTGGCCAACATTTCCTCAACTCTACATTGAAGGAGAGTTTTTCGGAGGCTGTGACATCACTGTTGATGCATATAAAAGCGGGGAGTTGCAGGAACTATTGGAGAAGGCAATGTGCTCGTAA

Protein Analysis

183

Amino Acids

19.89

Weight (kDa)

8.46

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 93 - 156 9.9e-21 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54900
fragaria_vesca FvH4_6g00720 FvH4_6g00720
malus_domestica MD04G1241900.v1.1 MD12G1258800.v1.1
prunus_persica Prupe.6G359300_v2.0.a1 Prupe.6G359300_v2.0.a1
pyrus_communis pycom04g21340 pycom12g23730
rosa_chinensis RchiOBHm_Chr3g0448191
rosa_laevigata RLG00000025939
rosa_multiflora Rmu_sc0013667.1_g000004
rosa_roxburghii Rroxscaffold_6G00426330
rosa_rugosa Rorug02G0608500
rosa_samantha Rh3AG008700 Rh3BG008200 Rh3CG008300 Rh3DG008900
rosa_wichuraiana Rw3G000690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 116
AccB7I CCANNNNNTGG 1 cut(s) 317
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 3 cut(s) 144, 222, 507
AclWI GGATC 1 cut(s) 72
AcoI YGGCCR 1 cut(s) 431
AcuI CTGAAG 1 cut(s) 260
AcyI GRCGYC 1 cut(s) 127
AdeI CACNNNGTG 2 cut(s) 277, 317
AfaI GTAC 3 cut(s) 339, 363, 422
AfiI CCNNNNNNNGG 2 cut(s) 62, 317
AgsI TTSAA 4 cut(s) 327, 358, 415, 458
AjnI CCWGG 1 cut(s) 405
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 3 cut(s) 68, 184, 238
AluI AGCT 3 cut(s) 68, 184, 238
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 1 cut(s) 154
AlwI GGATC 1 cut(s) 72
Ama87I CYCGRG 1 cut(s) 424
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 2 cut(s) 15, 431
ApeKI GCWGC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 2 cut(s) 15, 176
AsuII TTCGAA 2 cut(s) 191, 369
AvaI CYCGRG 1 cut(s) 424
AvaII GGWCC 1 cut(s) 176
BalI TGGCCA 1 cut(s) 433
BanI GGYRCC 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 1 cut(s) 55
BceAI ACGGC 1 cut(s) 131
BcgI CGANNNNNNTGC 1 cut(s) 525
BciT130I CCWGG 1 cut(s) 407
BcoDI GTCTC 1 cut(s) 154
BfaI CTAG 1 cut(s) 386
BisI GCNGC 2 cut(s) 69, 222
BlsI GCNGC 2 cut(s) 70, 223
BmcAI AGTACT 1 cut(s) 422
Bme1390I CCNGG 1 cut(s) 407
Bme18I GGWCC 1 cut(s) 176
BmeT110I CYCGRG 1 cut(s) 424
BmgT120I GGNCC 2 cut(s) 15, 176
BmiI GGNNCC 3 cut(s) 16, 118, 178
BmrFI CCNGG 1 cut(s) 407
BmsI GCATC 2 cut(s) 203, 484
BpiI GAAGAC 1 cut(s) 203
BpmI CTGGAG 1 cut(s) 84
Bpu14I TTCGAA 2 cut(s) 191, 369
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 3 cut(s) 18, 112, 406
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 317
Bse3DI GCAATG 1 cut(s) 543
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 1 cut(s) 407
BseDI CCNNGG 3 cut(s) 18, 112, 406
BseLI CCNNNNNNNGG 2 cut(s) 62, 317
BseMI GCAATG 1 cut(s) 543
BseXI GCAGC 1 cut(s) 55
BshFI GGCC 2 cut(s) 17, 433
BshNI GGYRCC 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 545
BsiHKCI CYCGRG 1 cut(s) 424
BsiSI CCGG 1 cut(s) 233
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 2 cut(s) 62, 317
BsmAI GTCTC 1 cut(s) 154
BsmBI CGTCTC 1 cut(s) 154
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 2 cut(s) 17, 433
BsoBI CYCGRG 1 cut(s) 424
Bsp119I TTCGAA 2 cut(s) 191, 369
Bsp1286I GDGCHC 1 cut(s) 545
Bsp13I TCCGGA 1 cut(s) 232
Bsp143I GATC 1 cut(s) 77
BspACI CCGC 3 cut(s) 144, 222, 507
BspANI GGCC 2 cut(s) 17, 433
BspEI TCCGGA 1 cut(s) 232
BspLI GGNNCC 3 cut(s) 16, 118, 178
BspPI GGATC 1 cut(s) 72
BspT104I TTCGAA 2 cut(s) 191, 369
BspT107I GGYRCC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 543
BssECI CCNNGG 3 cut(s) 18, 112, 406
BssMI GATC 1 cut(s) 77
BssNI GRCGYC 1 cut(s) 127
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 4 cut(s) 34, 116, 366, 490
Bst6I CTCTTC 1 cut(s) 193
BstACI GRCGYC 1 cut(s) 127
BstBI TTCGAA 2 cut(s) 191, 369
BstC8I GCNNGC 1 cut(s) 73
BstDSI CCRYGG 1 cut(s) 112
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 1 cut(s) 80
BstMAI GTCTC 1 cut(s) 154
BstMBI GATC 1 cut(s) 77
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 407
BstSCI CCNGG 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 55
BstV2I GAAGAC 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
BsuRI GGCC 2 cut(s) 17, 433
BtgI CCRYGG 1 cut(s) 112
BtsIMutI CAGTG 1 cut(s) 486
Cac8I GCNNGC 1 cut(s) 73
CfoI GCGC 1 cut(s) 405
Cfr13I GGNCC 2 cut(s) 15, 176
CseI GACGC 1 cut(s) 116
Csp6I GTAC 3 cut(s) 338, 362, 421
CviJI RGCY 6 cut(s) 17, 68, 184, 238, 433, 477
CviKI_1 RGCY 6 cut(s) 17, 68, 184, 238, 433, 477
CviQI GTAC 3 cut(s) 338, 362, 421
DpnI GATC 1 cut(s) 79
DpnII GATC 1 cut(s) 77
DraIII CACNNNGTG 2 cut(s) 277, 317
EaeI YGGCCR 1 cut(s) 431
Eam1104I CTCTTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 193
EciI GGCGGA 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 424
EcoO109I RGGNCCY 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 405
EcoT14I CCWWGG 1 cut(s) 18
EcoT22I ATGCAT 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 18
Esp3I CGTCTC 1 cut(s) 154
FaiI YATR 5 cut(s) 160, 290, 383, 499, 501
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FaqI GGGAC 1 cut(s) 114
FauI CCCGC 1 cut(s) 500
Fnu4HI GCNGC 2 cut(s) 69, 222
Fsp4HI GCNGC 2 cut(s) 69, 222
FspBI CTAG 1 cut(s) 386
GlaI GCGC 1 cut(s) 404
GluI GCNGC 2 cut(s) 69, 222
GsuI CTGGAG 1 cut(s) 84
HaeIII GGCC 2 cut(s) 17, 433
HapII CCGG 1 cut(s) 233
HgaI GACGC 1 cut(s) 116
HhaI GCGC 1 cut(s) 405
Hin1I GRCGYC 1 cut(s) 127
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HinfI GANTC 1 cut(s) 23
HpaII CCGG 1 cut(s) 233
Hpy188I TCNGA 3 cut(s) 176, 212, 473
Hpy188III TCNNGA 1 cut(s) 233
HpyAV CCTTC 6 cut(s) 150, 181, 286, 409, 452, 526
HpyCH4III ACNGT 4 cut(s) 34, 116, 366, 490
HpyCH4V TGCA 2 cut(s) 497, 517
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
Hsp92I GRCGYC 1 cut(s) 127
HspAI GCGC 1 cut(s) 403
Kpn2I TCCGGA 1 cut(s) 232
Kzo9I GATC 1 cut(s) 77
LmnI GCTCC 2 cut(s) 65, 235
LpnPI CCDG 6 cut(s) 48, 145, 246, 392, 419, 503
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 2 cut(s) 203, 484
MaeI CTAG 1 cut(s) 386
MaeIII GTNAC 3 cut(s) 34, 262, 479
MalI GATC 1 cut(s) 79
MboI GATC 1 cut(s) 77
MboII GAAGA 4 cut(s) 180, 205, 208, 253
MflI RGATCY 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 545
MlsI TGGCCA 1 cut(s) 433
MluCI AATT 1 cut(s) 88
MluNI TGGCCA 1 cut(s) 433
MlyI GAGTC 1 cut(s) 17
MnlI CCTC 9 cut(s) 58, 66, 104, 130, 147, 212, 342, 453, 467
Mox20I TGGCCA 1 cut(s) 433
Mph1103I ATGCAT 1 cut(s) 499
MroI TCCGGA 1 cut(s) 232
MscI TGGCCA 1 cut(s) 433
MseI TTAA 1 cut(s) 227
MslI CAYNNNNRTG 1 cut(s) 211
Msp20I TGGCCA 1 cut(s) 433
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 1 cut(s) 407
MvaI CCWGG 1 cut(s) 407
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 1 cut(s) 77
NlaIV GGNNCC 3 cut(s) 16, 118, 178
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 1 cut(s) 479
NsiI ATGCAT 1 cut(s) 499
NspV TTCGAA 2 cut(s) 191, 369
PaeR7I CTCGAG 1 cut(s) 424
PflMI CCANNNNNTGG 1 cut(s) 317
PkrI GCNGC 2 cut(s) 70, 223
PleI GAGTC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 405
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 3 cut(s) 16, 118, 178
PspPI GGNCC 2 cut(s) 15, 176
PspXI VCTCGAGB 1 cut(s) 424
PsuI RGATCY 1 cut(s) 77
RsaI GTAC 3 cut(s) 339, 363, 422
RsaNI GTAC 3 cut(s) 338, 362, 421
RseI CAYNNNNRTG 1 cut(s) 211
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 2 cut(s) 69, 222
Sau3AI GATC 1 cut(s) 77
Sau96I GGNCC 2 cut(s) 15, 176
ScaI AGTACT 1 cut(s) 422
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 1 cut(s) 407
SduI GDGCHC 1 cut(s) 545
SetI ASST 4 cut(s) 70, 186, 240, 353
SfaNI GCATC 2 cut(s) 203, 484
Sfr274I CTCGAG 1 cut(s) 424
SfuI TTCGAA 2 cut(s) 191, 369
SinI GGWCC 1 cut(s) 176
SlaI CTCGAG 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 211
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
Sse9I AATT 1 cut(s) 88
SsiI CCGC 3 cut(s) 144, 222, 507
SspMI CTAG 1 cut(s) 386
StyD4I CCNGG 1 cut(s) 405
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 4 cut(s) 34, 116, 366, 490
TaqI TCGA 3 cut(s) 191, 369, 425
TasI AATT 1 cut(s) 88
TatI WGTACW 1 cut(s) 420
TauI GCSGC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 3 cut(s) 147, 305, 408
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 1 cut(s) 493
Van91I CCANNNNNTGG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 176
XhoI CTCGAG 1 cut(s) 424
XspI CTAG 1 cut(s) 386
ZrmI AGTACT 1 cut(s) 422
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.