MD05G1001600.v1.1
ERF Family

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012009
Physical Location & Seq
Forward (+)
811376 .. 821703
10328 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1001600.v1.1.491

Sequence Viewer

Length: 2784 bp
ATGGAGGCGATCGAGGAATTGTTGCAGCTATCAGACTCGATGCGGCAAGGCGCTGCTATCCTTGCCGACGAAGATGTTGACGAGAACACAACCTCCTCTCGCAGGGCTTCCAGTTTCCTTAACGTCGTCGCTCTCGGCAATGTTGGTGCGGGTAAATCAGCAGTTTTGAACAGTTTGATTGGACATCCTGTTTTGCCAACTGGTGAAAATGGTGCTACTCGAGCTCCCATTAGCATTGATTTGCAGAGGGATGGCTCCTTGAGCAGCAAATCTATTATATTGCAGATTGACAATAAATCTCAGCAAGTTTCTGCAAGTGCTTTACGGCATTCTCTGCAAGGTAGGCTGGGAAACGCCACAGGAAAAACCCGTGATGAAATTAATTTGAAACTACGAACAAGTACAGCCCCACCCCTCAAATTGATTGATTTACCTGGGCTTGATCAAAGGATCATGGATGAGTCTATGATTAGTGAATATGCTGAGCACAATGATTCAATTTTGCTCGTTATAGTTCCTGCATCCCAAGCACCTGAAGTTGCTTCATATCGAGCCCTTAGAGTCGTCAAGGAGTATGATGCAGATGGTACCAGAACTGTTGGTGTTATTAGCAAGATAGACCAAGCTGCTTCAGATCAGAAAGCACTTGCTGCAGTCCAGGCTCTTCTGTTAAATCAAGGACCATCAAGAGCATCTGATATCCCTTGGGTTGCTTTAATAGGTCAATCGGTTTCAATTGCTTCTGCACAGTCTGGAGGTTCTGAAAGTTCTCTAGAAACTGCCTGGAGGGCTGAAAGTGAAAGTCTGAAGTCTATTTTGACCGGAGCTCCTCAAAGTAAGCTAGGAAGAATAGCGTTGGTGGATGCTCTGGCACAGCAGATTCGTAGCCGTATGAAAGTTCGACTTCCAAACCTCCTTTCTGGGTTACAAGGGAAGTCTCAAATAGTTCAGGATGAGTTAGTAAAGCTTGGTGCATCTATGGTGCAGAGTGCTGAGGGTACCAGATCACTAGCTTTGGAGCTTTGCCGTGAGTTTGAGGATAAGTTTCTCCAACACATTACAACTGGTGAGGGTTCTGGATGGAAAATTGTTGGAAGTTTTGAAGGAAATTTCCCAAATAGAATAAAACAACTCCCATTAGACCGACATTTTGATATCAATAATGTGAAAAGGATTGTGTTGGAAGCAGATGGTTATCAACCTTATCTCATATCCCCTGAAAAGGGGTTGAGGTCTTTAATAAAAGGGGTCTTAGAGCTTGCAAAGGAGCCATCACGTCTCTGTGTGGATGAGGTACATCGTGTCCTGGTAGATATAGTTTCTGCTGCTGCAAATGCTACGTCAGGTCTTGGAAGATACCCTCCTTTCAAGAGGGAGGTTGTGGCCATTGCAAGTGCTGCATTGGATGCGTTTAAGAATGAAGCAAAGAAAATGGTGGTTGCACTGGTAGATATGGAGCGTGCTTTTGTGCCTCCCCAACACTTCATTCGCTTGGTGCAAAGGAGGATGGAGAGGCAGCGTAGAGAGGAAGAAGTGAAAAATCGATCTTCAAAGAAAGGACAAGAGGCAGAGCAATCAATAATGAATCGGGCAACGAGTCCTCAAACAGGGGGGGAGCAAGCTGGTGGAAGCATGAAATCAATGAAGGATAAGGATAAATCTGGTAAGGACGGCAAAGAAGTACAGGAGGCAACTGGTTTGAAGACCGCAGGTCCTGAAGGAGAAATAACAGCAGGTTTCTTGTATAAAAAAAGTGCCAAAACTAATGGATGGAGCAAGCGATGGTTTGTTTTGAATGAAAAGACTGGAAAGCTTGGTTACACCAAAAAGCAAGAGGAGAGACACTTCCGTGGTGTCATCACTTTGGAGGAGTGTAATATTGAAGAGGCTTCAGATGAGGATGAACCACCTCCATCAAAGAGCTCAAAGGACAAAAAGTCAAATGGACCAGAAAAATCACCGAGTCTTGTGTTTAAGATAACTAGCAAAGTTCCATATAAAACTGTTCTGAAAGCGCACAGTGCCGTTGTGCTAAAGGCAGAGACTGTGGCTGACAAGCTAGAGTGGATAAATAAGATTGGAAAGGTTATTCAACCTTCTAAAGGACTAATGAGGGGTGCTTCAACTGAAGGCGGTCCTACAATGCGACAAAGTCTTTCTGATGGTTCTCTTGATACAATGACTCGAAGACCGGCTGACCCTGAGGAAGAACTCCGGTGGATGTCTCAAGAAGTTCGTGGTTATGTTGAAGCTGTTTTGAATAGTTTGGCTGCTAATGTCCCTAAGGCAGTTGTTCTTTGCCAAGTTGAAAAGGCCAAGGAAGACATGTTAAATCAGCTATATAGCTCTGTCAGTGCTCAAAGCACAGCAAAGATTGAAGAGCTGCTTCAGGAGGATCAGAATGTGAAGCGGAGGAGGGAGCGCTACCAGAAACAGTCATCACTCCTTTCCAAACTAACACGCCAACTGAGCATTCATGACAACCGAGCAGCTGCTGCATCCAGCTGGTCCAATGGTGGTAGTGGTGCACCAGAAAGCAGCCCAAGATCTTCATCCAGTGGTGCATCAGGTGATGACTGGAGATCCGCCTTTGATGCCGCCGCTAATGGCCCAGTTGACCGTAGTTCTTCATTATCTAGATCTTCATCCAATGGTCACAGCCGACATTATAGTGATCCAGCACAAAATGGTGATGTGAACTCTGGTCCAAACTCTGGTAGCCGTCGTACTCCAAATCGGTTGCCACCTGCACCGCCAGGTTCTTCAGGCAGCAGATACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000266 GO:0000902 GO:0000904 GO:0001101 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006892 GO:0006896 GO:0006897 GO:0006898 GO:0006996 GO:0007005 GO:0007034 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0009504 GO:0009506 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0012505 GO:0015631 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0021700 GO:0022622 GO:0030054 GO:0030135 GO:0030136 GO:0030139 GO:0030154 GO:0030276 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032878 GO:0032989 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045334 GO:0046907 GO:0048193 GO:0048285 GO:0048364 GO:0048468 GO:0048469 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055044 GO:0065007 GO:0071695 GO:0071840 GO:0071944 GO:0072583 GO:0090558 GO:0090627 GO:0097305 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0099402 GO:1901700 GO:1905392 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko04721 ko04961 ko05100 map04072 map04144 map04721 map04961 map05100
Pfam Domains
Protein Families
Dynamin_M Dynamin_N GED PH

Protein Analysis

928

Amino Acids

100.5

Weight (kDa)

9.15

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_N PF00350 42 - 205 1.2e-29 Dynamin family
Dynamin_M PF01031 257 - 494 1.5e-23 Dynamin central region
PH PF00169 578 - 697 5.8e-11 PH domain
PH_8 PF15409 580 - 631 5.6e-06 Pleckstrin homology domain
GED PF02212 736 - 818 2.2e-13 Dynamin GTPase effector domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10290 AT1G59610
fragaria_vesca FvH4_2g00460 FvH4_2g00460
malus_domestica MD05G1001600.v1.1 MD10G1002800.v1.1
prunus_persica Prupe.8G004400_v2.0.a1
pyrus_communis pycom05g00170 pycom10g00220
rosa_chinensis RchiOBHm_Chr6g0250411
rosa_laevigata RLG00000015541
rosa_multiflora Rmu_sc0001209.1_g000036
rosa_roxburghii Rroxscaffold_7G00217250
rosa_rugosa Rorug05G0496400 Rorug05G0496400
rosa_samantha Rh6AG005800 Rh6DG003800
rosa_wichuraiana Rw6G000640 Rw6G000710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2755
Acc36I ACCTGC 3 cut(s) 1700, 1724, 2755
Acc65I GGTACC 2 cut(s) 587, 998
AccB1I GGYRCC 2 cut(s) 587, 998
AccB7I CCANNNNNTGG 1 cut(s) 2714
AciI CCGC 9 cut(s) 43, 149, 1707, 2133, 2410, 2586, 2598, 2601, 2753
AclWI GGATC 4 cut(s) 458, 2403, 2577, 2669
AcoI YGGCCR 1 cut(s) 1383
AcsI RAATTY 1 cut(s) 1108
AcuI CTGAAG 8 cut(s) 555, 615, 827, 1737, 1875, 2148, 2372, 2748
AfaI GTAC 6 cut(s) 403, 589, 1000, 1296, 1683, 2728
AfeI AGCGCT 1 cut(s) 2423
AfiI CCNNNNNNNGG 6 cut(s) 102, 1222, 1223, 1607, 2102, 2714
AflIII ACRYGT 1 cut(s) 2325
AhdI GACNNNNNGTC 2 cut(s) 1710, 1936
AjiI CACGTC 1 cut(s) 1277
AjnI CCWGG 5 cut(s) 433, 657, 782, 1305, 2755
AjuI GAANNNNNNNTTGG 6 cut(s) 901, 933, 1470, 1502, 2457, 2489
AleI CACNNNNGTG 1 cut(s) 1848
AloI GAACNNNNNNTCC 2 cut(s) 77, 109
Alw21I GWGCWC 6 cut(s) 226, 489, 829, 1925, 2359, 2530
Alw26I GTCTC 5 cut(s) 942, 1283, 1834, 2036, 2229
Alw44I GTGCAC 1 cut(s) 2526
AlwI GGATC 4 cut(s) 458, 2403, 2577, 2669
AlwNI CAGNNNCTG 3 cut(s) 1715, 2045, 2495
Ama87I CYCGRG 1 cut(s) 219
Aor51HI AGCGCT 1 cut(s) 2423
AoxI GGCC 3 cut(s) 1383, 2313, 2608
ApaLI GTGCAC 1 cut(s) 2526
ApoI RAATTY 1 cut(s) 1108
ArsI GACNNNNNNTTYG 4 cut(s) 1133, 1165, 1325, 1357
AseI ATTAAT 1 cut(s) 381
Asp718I GGTACC 2 cut(s) 587, 998
AspLEI GCGC 3 cut(s) 53, 2017, 2424
AspS9I GGNCC 7 cut(s) 680, 1712, 1946, 2135, 2508, 2609, 2705
AsuHPI GGTGA 5 cut(s) 215, 1079, 1950, 2582, 2702
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 6 cut(s) 680, 1712, 1946, 2135, 2508, 2705
AxyI CCTNAGG 2 cut(s) 2202, 2283
BaeGI GKGCMC 1 cut(s) 2530
BalI TGGCCA 1 cut(s) 1385
BanI GGYRCC 2 cut(s) 587, 998
BanII GRGCYC 4 cut(s) 226, 556, 829, 1925
BbsI GAAGAC 3 cut(s) 1709, 2194, 2328
Bbv12I GWGCWC 6 cut(s) 226, 489, 829, 1925, 2359, 2530
BbvCI CCTCAGC 1 cut(s) 993
BceAI ACGGC 6 cut(s) 341, 873, 1011, 1687, 2009, 2706
BciT130I CCWGG 5 cut(s) 435, 659, 784, 1307, 2757
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 5 cut(s) 942, 1283, 1834, 2036, 2229
BfaI CTAG 6 cut(s) 773, 842, 1010, 1983, 2060, 2637
BfmI CTRYAG 1 cut(s) 651
BfoI RGCGCY 2 cut(s) 54, 2425
BfuAI ACCTGC 3 cut(s) 1700, 1724, 2755
BglI GCCNNNNNGGC 1 cut(s) 788
BglII AGATCT 2 cut(s) 2546, 2639
BlpI GCTNAGC 1 cut(s) 483
Bme1390I CCNGG 5 cut(s) 435, 659, 784, 1307, 2757
Bme18I GGWCC 6 cut(s) 680, 1712, 1946, 2135, 2508, 2705
BmeRI GACNNNNNGTC 2 cut(s) 1710, 1936
BmeT110I CYCGRG 1 cut(s) 219
BmgBI CACGTC 1 cut(s) 1277
BmgT120I GGNCC 7 cut(s) 680, 1712, 1946, 2135, 2508, 2609, 2705
BmiI GGNNCC 4 cut(s) 256, 589, 1000, 1269
BmrFI CCNGG 5 cut(s) 435, 659, 784, 1307, 2757
BmrI ACTGGG 1 cut(s) 2606
BmuI ACTGGG 1 cut(s) 2606
BpiI GAAGAC 3 cut(s) 1709, 2194, 2328
BpmI CTGGAG 3 cut(s) 774, 805, 2599
Bpu10I CCTNAGC 1 cut(s) 993
Bpu1102I GCTNAGC 1 cut(s) 483
BpuEI CTTGAG 2 cut(s) 280, 2211
Bsa29I ATCGAT 1 cut(s) 1543
BsaBI GATNNNNATC 3 cut(s) 1194, 2551, 2644
BsaJI CCNNGG 4 cut(s) 434, 704, 1849, 2316
BsaWI WCCGGW 2 cut(s) 821, 2214
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 208, 238, 811, 841
Bsc4I CCNNNNNNNGG 6 cut(s) 102, 1222, 1223, 1607, 2102, 2714
Bse118I RCCGGY 1 cut(s) 2191
Bse1I ACTGG 9 cut(s) 111, 205, 1069, 1449, 1699, 1810, 2556, 2582, 2612
Bse21I CCTNAGG 2 cut(s) 2202, 2283
Bse3DI GCAATG 2 cut(s) 145, 1386
Bse8I GATNNNNATC 3 cut(s) 1194, 2551, 2644
BseBI CCWGG 5 cut(s) 435, 659, 784, 1307, 2757
BseCI ATCGAT 1 cut(s) 1543
BseDI CCNNGG 4 cut(s) 434, 704, 1849, 2316
BseJI GATNNNNATC 3 cut(s) 1194, 2551, 2644
BseLI CCNNNNNNNGG 6 cut(s) 102, 1222, 1223, 1607, 2102, 2714
BseMI GCAATG 2 cut(s) 145, 1386
BseMII CTCAG 5 cut(s) 314, 474, 984, 2193, 2459
BseNI ACTGG 9 cut(s) 111, 205, 1069, 1449, 1699, 1810, 2556, 2582, 2612
BseRI GAGGAG 5 cut(s) 85, 819, 1850, 1883, 2428
BseSI GKGCMC 1 cut(s) 2530
BseYI CCCAGC 1 cut(s) 346
BsgI GTGCAG 3 cut(s) 729, 1004, 2733
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 1385, 2315, 2610
BshNI GGYRCC 2 cut(s) 587, 998
BshVI ATCGAT 1 cut(s) 1543
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 6 cut(s) 226, 489, 829, 1925, 2359, 2530
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 3 cut(s) 822, 2192, 2215
BslFI GGGAC 1 cut(s) 2264
BslI CCNNNNNNNGG 6 cut(s) 102, 1222, 1223, 1607, 2102, 2714
BsmAI GTCTC 5 cut(s) 942, 1283, 1834, 2036, 2229
BsmBI CGTCTC 1 cut(s) 1283
BsmFI GGGAC 1 cut(s) 2264
BsmI GAATGC 2 cut(s) 328, 2472
BsnI GGCC 3 cut(s) 1385, 2315, 2610
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 7 cut(s) 226, 489, 556, 829, 1925, 2359, 2530
Bsp1720I GCTNAGC 1 cut(s) 483
BspACI CCGC 9 cut(s) 43, 149, 1707, 2133, 2410, 2586, 2598, 2601, 2753
BspANI GGCC 3 cut(s) 1385, 2315, 2610
BspCNI CTCAG 5 cut(s) 313, 475, 985, 2194, 2460
BspDI ATCGAT 1 cut(s) 1543
BspHI TCATGA 1 cut(s) 2476
BspLI GGNNCC 4 cut(s) 256, 589, 1000, 1269
BspMAI CTGCAG 1 cut(s) 655
BspMI ACCTGC 3 cut(s) 1700, 1724, 2755
BspPI GGATC 4 cut(s) 458, 2403, 2577, 2669
BspQI GCTCTTC 2 cut(s) 669, 2373
BspT107I GGYRCC 2 cut(s) 587, 998
BsrDI GCAATG 2 cut(s) 145, 1386
BsrFI RCCGGY 1 cut(s) 2191
BsrI ACTGG 9 cut(s) 111, 205, 1069, 1449, 1699, 1810, 2556, 2582, 2612
BssAI RCCGGY 1 cut(s) 2191
BssECI CCNNGG 4 cut(s) 434, 704, 1849, 2316
BssT1I CCWWGG 2 cut(s) 704, 2316
Bst2UI CCWGG 5 cut(s) 435, 659, 784, 1307, 2757
Bst4CI ACNGT 8 cut(s) 173, 598, 750, 2005, 2021, 2047, 2436, 2621
Bst6I CTCTTC 3 cut(s) 669, 1878, 2373
BstAPI GCANNNNNTGC 5 cut(s) 334, 650, 1397, 1406, 2495
BstC8I GCNNGC 4 cut(s) 1260, 1461, 1620, 1778
BstDEI CTNAG 8 cut(s) 300, 483, 557, 993, 1252, 2202, 2283, 2468
BstDSI CCRYGG 1 cut(s) 1849
BstENI CCTNNNNNAGG 3 cut(s) 100, 1605, 2100
BstH2I RGCGCY 2 cut(s) 54, 2425
BstHHI GCGC 3 cut(s) 53, 2017, 2424
BstMAI GTCTC 5 cut(s) 942, 1283, 1834, 2036, 2229
BstMCI CGRYCG 1 cut(s) 12
BstNI CCWGG 5 cut(s) 435, 659, 784, 1307, 2757
BstNSI RCATGY 1 cut(s) 2329
BstSCI CCNGG 5 cut(s) 433, 657, 782, 1305, 2755
BstSFI CTRYAG 1 cut(s) 651
BstSLI GKGCMC 1 cut(s) 2530
BstV2I GAAGAC 3 cut(s) 1709, 2194, 2328
BstX2I RGATCY 3 cut(s) 2546, 2582, 2639
BstYI RGATCY 3 cut(s) 2546, 2582, 2639
Bsu15I ATCGAT 1 cut(s) 1543
Bsu36I CCTNAGG 2 cut(s) 2202, 2283
BsuRI GGCC 3 cut(s) 1385, 2315, 2610
BsuTUI ATCGAT 1 cut(s) 1543
BtgI CCRYGG 1 cut(s) 1849
BtgZI GCGATG 1 cut(s) 1795
BtrI CACGTC 1 cut(s) 1277
BtsIMutI CAGTG 4 cut(s) 1442, 2026, 2359, 2563
BveI ACCTGC 3 cut(s) 1700, 1724, 2755
Cac8I GCNNGC 4 cut(s) 1260, 1461, 1620, 1778
CaiI CAGNNNCTG 3 cut(s) 1715, 2045, 2495
CciI TCATGA 1 cut(s) 2476
CfoI GCGC 3 cut(s) 53, 2017, 2424
Cfr10I RCCGGY 1 cut(s) 2191
Cfr13I GGNCC 7 cut(s) 680, 1712, 1946, 2135, 2508, 2609, 2705
ClaI ATCGAT 1 cut(s) 1543
Csp6I GTAC 6 cut(s) 402, 588, 999, 1295, 1682, 2727
CviAII CATG 4 cut(s) 454, 1633, 2326, 2477
CviQI GTAC 6 cut(s) 402, 588, 999, 1295, 1682, 2727
DdeI CTNAG 8 cut(s) 300, 483, 557, 993, 1252, 2202, 2283, 2468
DriI GACNNNNNGTC 2 cut(s) 1710, 1936
EaeI YGGCCR 1 cut(s) 1383
Eam1104I CTCTTC 3 cut(s) 669, 1878, 2373
Eam1105I GACNNNNNGTC 2 cut(s) 1710, 1936
EarI CTCTTC 3 cut(s) 669, 1878, 2373
EciI GGCGGA 1 cut(s) 2575
Ecl136II GAGCTC 3 cut(s) 224, 827, 1923
Eco130I CCWWGG 2 cut(s) 704, 2316
Eco24I GRGCYC 4 cut(s) 226, 556, 829, 1925
Eco32I GATATC 2 cut(s) 700, 1156
Eco47I GGWCC 6 cut(s) 680, 1712, 1946, 2135, 2508, 2705
Eco47III AGCGCT 1 cut(s) 2423
Eco53kI GAGCTC 3 cut(s) 224, 827, 1923
Eco57I CTGAAG 8 cut(s) 555, 615, 827, 1737, 1875, 2148, 2372, 2748
Eco81I CCTNAGG 2 cut(s) 2202, 2283
Eco88I CYCGRG 1 cut(s) 219
EcoICRI GAGCTC 3 cut(s) 224, 827, 1923
EcoNI CCTNNNNNAGG 3 cut(s) 100, 1605, 2100
EcoO109I RGGNCCY 1 cut(s) 1712
EcoRII CCWGG 5 cut(s) 433, 657, 782, 1305, 2755
EcoRV GATATC 2 cut(s) 700, 1156
EcoT14I CCWWGG 2 cut(s) 704, 2316
EcoT38I GRGCYC 4 cut(s) 226, 556, 829, 1925
ErhI CCWWGG 2 cut(s) 704, 2316
Esp3I CGTCTC 1 cut(s) 1283
FaeI CATG 4 cut(s) 457, 1636, 2329, 2480
FalI AAGNNNNNCTT 4 cut(s) 888, 920, 2370, 2402
FaqI GGGAC 1 cut(s) 2264
FatI CATG 4 cut(s) 453, 1632, 2325, 2476
FauI CCCGC 1 cut(s) 142
FbaI TGATCA 1 cut(s) 442
FriOI GRGCYC 4 cut(s) 226, 556, 829, 1925
FspBI CTAG 6 cut(s) 773, 842, 1010, 1983, 2060, 2637
GlaI GCGC 3 cut(s) 52, 2016, 2423
GsaI CCCAGC 1 cut(s) 350
GsuI CTGGAG 3 cut(s) 774, 805, 2599
HaeII RGCGCY 2 cut(s) 54, 2425
HaeIII GGCC 3 cut(s) 1385, 2315, 2610
HapII CCGG 3 cut(s) 822, 2192, 2215
HhaI GCGC 3 cut(s) 53, 2017, 2424
Hin1II CATG 4 cut(s) 457, 1636, 2329, 2480
Hin6I GCGC 3 cut(s) 51, 2015, 2422
HinP1I GCGC 3 cut(s) 51, 2015, 2422
HincII GTYRAC 2 cut(s) 79, 2617
HindII GTYRAC 2 cut(s) 79, 2617
HindIII AAGCTT 2 cut(s) 965, 1811
HinfI GANTC 9 cut(s) 35, 461, 494, 561, 880, 1585, 1597, 1963, 2182
HpaII CCGG 3 cut(s) 822, 2192, 2215
HphI GGTGA 5 cut(s) 215, 1079, 1950, 2582, 2702
Hpy166II GTNNAC 4 cut(s) 79, 2528, 2617, 2698
Hpy8I GTNNAC 4 cut(s) 79, 2528, 2617, 2698
Hpy99I CGWCG 4 cut(s) 71, 128, 131, 2727
HpyAV CCTTC 5 cut(s) 1097, 1639, 1712, 2106, 2123
HpyCH4III ACNGT 8 cut(s) 173, 598, 750, 2005, 2021, 2047, 2436, 2621
HpyCH4IV ACGT 3 cut(s) 123, 1276, 1340
HpyF3I CTNAG 8 cut(s) 300, 483, 557, 993, 1252, 2202, 2283, 2468
HpySE526I ACGT 3 cut(s) 123, 1276, 1340
Hsp92II CATG 4 cut(s) 457, 1636, 2329, 2480
HspAI GCGC 3 cut(s) 51, 2015, 2422
KpnI GGTACC 2 cut(s) 591, 1002
Ksp22I TGATCA 1 cut(s) 442
LguI GCTCTTC 2 cut(s) 669, 2373
MaeI CTAG 6 cut(s) 773, 842, 1010, 1983, 2060, 2637
MaeII ACGT 3 cut(s) 123, 1276, 1340
MaeIII GTNAC 3 cut(s) 924, 1817, 2654
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 3 cut(s) 2546, 2582, 2639
MhlI GDGCHC 7 cut(s) 226, 489, 556, 829, 1925, 2359, 2530
MlsI TGGCCA 1 cut(s) 1385
MluCI AATT 8 cut(s) 17, 378, 382, 419, 498, 735, 1086, 1108
MluNI TGGCCA 1 cut(s) 1385
MlyI GAGTC 6 cut(s) 29, 470, 570, 1606, 1972, 2176
MmeI TCCRAC 3 cut(s) 1072, 1075, 1161
Mox20I TGGCCA 1 cut(s) 1385
MscI TGGCCA 1 cut(s) 1385
MseI TTAA 8 cut(s) 120, 381, 671, 716, 1238, 1413, 1974, 2330
MslI CAYNNNNRTG 2 cut(s) 1848, 2670
Msp20I TGGCCA 1 cut(s) 1385
MspA1I CMGCKG 2 cut(s) 2492, 2505
MspI CCGG 3 cut(s) 822, 2192, 2215
MspR9I CCNGG 5 cut(s) 435, 659, 784, 1307, 2757
MunI CAATTG 1 cut(s) 735
Mva1269I GAATGC 2 cut(s) 328, 2472
MvaI CCWGG 5 cut(s) 435, 659, 784, 1307, 2757
NlaIII CATG 4 cut(s) 457, 1636, 2329, 2480
NlaIV GGNNCC 4 cut(s) 256, 589, 1000, 1269
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 1 cut(s) 2654
NspI RCATGY 1 cut(s) 2329
OliI CACNNNNGTG 1 cut(s) 1848
PaeR7I CTCGAG 1 cut(s) 219
PagI TCATGA 1 cut(s) 2476
PaqCI CACCTGC 1 cut(s) 2755
PciI ACATGT 1 cut(s) 2325
PciSI GCTCTTC 2 cut(s) 669, 2373
PctI GAATGC 2 cut(s) 328, 2472
PfeI GAWTC 3 cut(s) 494, 880, 1585
PflFI GACNNNGTC 1 cut(s) 2151
PflMI CCANNNNNTGG 1 cut(s) 2714
Ple19I CGATCG 1 cut(s) 12
PleI GAGTC 6 cut(s) 29, 469, 569, 1605, 1971, 2176
PpsI GAGTC 6 cut(s) 29, 469, 569, 1605, 1971, 2176
PpuMI RGGWCCY 1 cut(s) 1712
PscI ACATGT 1 cut(s) 2325
PshBI ATTAAT 1 cut(s) 381
Psp124BI GAGCTC 3 cut(s) 226, 829, 1925
Psp5II RGGWCCY 1 cut(s) 1712
Psp6I CCWGG 5 cut(s) 433, 657, 782, 1305, 2755
PspFI CCCAGC 1 cut(s) 346
PspGI CCWGG 5 cut(s) 433, 657, 782, 1305, 2755
PspN4I GGNNCC 4 cut(s) 256, 589, 1000, 1269
PspPI GGNCC 7 cut(s) 680, 1712, 1946, 2135, 2508, 2609, 2705
PspPPI RGGWCCY 1 cut(s) 1712
PspXI VCTCGAGB 1 cut(s) 219
PstI CTGCAG 1 cut(s) 655
PstNI CAGNNNCTG 3 cut(s) 1715, 2045, 2495
PsuI RGATCY 3 cut(s) 2546, 2582, 2639
PsyI GACNNNGTC 1 cut(s) 2151
PvuI CGATCG 1 cut(s) 12
PvuII CAGCTG 2 cut(s) 2492, 2505
RsaI GTAC 6 cut(s) 403, 589, 1000, 1296, 1683, 2728
RsaNI GTAC 6 cut(s) 402, 588, 999, 1295, 1682, 2727
RseI CAYNNNNRTG 2 cut(s) 1848, 2670
SacI GAGCTC 3 cut(s) 226, 829, 1925
SapI GCTCTTC 2 cut(s) 669, 2373
SaqAI TTAA 8 cut(s) 120, 381, 671, 716, 1238, 1413, 1974, 2330
Sau96I GGNCC 7 cut(s) 680, 1712, 1946, 2135, 2508, 2609, 2705
SchI GAGTC 6 cut(s) 29, 470, 570, 1606, 1972, 2176
ScrFI CCNGG 5 cut(s) 435, 659, 784, 1307, 2757
SduI GDGCHC 7 cut(s) 226, 489, 556, 829, 1925, 2359, 2530
SfcI CTRYAG 1 cut(s) 651
Sfr274I CTCGAG 1 cut(s) 219
SinI GGWCC 6 cut(s) 680, 1712, 1946, 2135, 2508, 2705
SlaI CTCGAG 1 cut(s) 219
SmiMI CAYNNNNRTG 2 cut(s) 1848, 2670
SmlI CTYRAG 3 cut(s) 219, 259, 2226
SmoI CTYRAG 3 cut(s) 219, 259, 2226
Sse9I AATT 8 cut(s) 17, 378, 382, 419, 498, 735, 1086, 1108
SsiI CCGC 9 cut(s) 43, 149, 1707, 2133, 2410, 2586, 2598, 2601, 2753
SspI AATATT 1 cut(s) 1879
SspMI CTAG 6 cut(s) 773, 842, 1010, 1983, 2060, 2637
SstI GAGCTC 3 cut(s) 226, 829, 1925
StyD4I CCNGG 5 cut(s) 433, 657, 782, 1305, 2755
StyI CCWWGG 2 cut(s) 704, 2316
TaaI ACNGT 8 cut(s) 173, 598, 750, 2005, 2021, 2047, 2436, 2621
TaiI ACGT 3 cut(s) 126, 1279, 1343
TaqI TCGA 7 cut(s) 12, 38, 220, 550, 901, 1543, 2185
TaqII GACCGA 1 cut(s) 1158
TasI AATT 8 cut(s) 17, 378, 382, 419, 498, 735, 1086, 1108
TatI WGTACW 2 cut(s) 401, 1681
TauI GCSGC 3 cut(s) 46, 2600, 2603
TfiI GAWTC 3 cut(s) 494, 880, 1585
Tru1I TTAA 8 cut(s) 120, 381, 671, 716, 1238, 1413, 1974, 2330
Tru9I TTAA 8 cut(s) 120, 381, 671, 716, 1238, 1413, 1974, 2330
TscAI CASTG 4 cut(s) 1449, 2026, 2359, 2563
TseFI GTSAC 1 cut(s) 2654
Tsp45I GTSAC 1 cut(s) 2654
TspGWI ACGGA 1 cut(s) 1838
TspRI CASTG 4 cut(s) 1449, 2026, 2359, 2563
Tth111I GACNNNGTC 1 cut(s) 2151
Van91I CCANNNNNTGG 1 cut(s) 2714
VneI GTGCAC 1 cut(s) 2526
VpaK11BI GGWCC 6 cut(s) 680, 1712, 1946, 2135, 2508, 2705
VspI ATTAAT 1 cut(s) 381
XagI CCTNNNNNAGG 3 cut(s) 100, 1605, 2100
XapI RAATTY 1 cut(s) 1108
XbaI TCTAGA 2 cut(s) 772, 2636
XceI RCATGY 1 cut(s) 2329
XhoI CTCGAG 1 cut(s) 219
XspI CTAG 6 cut(s) 773, 842, 1010, 1983, 2060, 2637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.