pycom10g00220
ERF Family

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012009
Physical Location & Seq
Forward (+)
332199 .. 342778
10580 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00220.1

Sequence Viewer

Length: 2697 bp
ATGGAGGCGATCGAGGAGTTGTTGCAGCTATCAGACTCGATGCGGCAAGGCGCCGCTATACTTGCCGACGAAGATGTTGACGAGAACACAACCTCCTCTCGCAGGGCTTCCAGTTTCCTTAACGTCGTCGCTCTCGGCAATGTTGGTGCGGGTAAATCAGCAGTTTTGAACAGTTTGATTGGACATCCTGTTTTGCCAACCGGTGAAAATGGTGCTACTCGAGCTCCCATTAGCATTGATTTGCAGAGGGATGGTTCTTTGAGCAGCAAATCTATTATACTGCAGATTGACAATAAATCTCAACAAGTTTCTGCAAGTGCTTTACGGCATTCACTGCAAGGTAGGCTGGGTAACGCTACAGGAAAAACCCGAGATGAAATTAATTTGAAACTACGGACAAGTACAGCCCCACCCCTCAAATTGATTGATTTACCTGGGCTTGATCAACGGATCATGGATGAGTCTATGATTAGTGAATATGCTGAGCACAATGATTCAATTTTGCTCGTTATAATACCTGCATCTCAAGCACCGGAAGTTGCTTCATCTCGAGCCCTCAGAGTTGTCAAGGAGTACGATGCAGATGGTACCAGAACCGTTGGTGTTATTAGCAAGATAGACCAAGCTGCTTCAGATCAGAAATCACTTGCTGCAGTCCAGGCACTTCTGTTAAATCAAGGGCCATCAAGGGCATCCGATATTCCTTGGGTTGCTTTGATAGGTCAATCGGTTGCAATAGCTTCTGCACAGTCTGGAGGTTCTGAAAGTTCCCTAGAAACTGCCTGGAGGGCTGAAAGCGAAAGTCTGAAGTCTATTTTGACCGGAGCTCCCCAAAGTAAGCTTGGCAGAATAGCCTTGGTGGATGCTCTGGCACAGCAGATTCGTAGCCGTATGAAAGTTCGACTTCCAAACCTCCTTTCTGGGTTACAAGGGAAGTCTCAAATAGTTCAGGATGAATTAGTGAAGCTTGGTGCGTCTATGGTGCAGAGTGCTGAGGGCACCAGATCTCTAGCTTTGGAGCTTTGCCGTGAGTTTGAGGATAAGTTTCTCCAACACATTACATCTGGTGAGGGTTCTGGATGGAAAATTGTTGCAAGTTTTGAAGGAAATTTTCCAAATAGAATAAAACAACTCCCATTAGACCGACATTTTGATATCAATAATGTGAAGAGGATTGTGTTGGAAGCAGATGGTTATCAACCATATCTCATATCCCCCGAAAAGGGGTTGAGATCTTTAATAAAAGGGGTCTTAGAGCTTGCAAAAGAACCATCACGTCTCTGTGTGGATGAGGTACACCGTGTCCTGGTAGATATAGTTTCTGCTGCTGCAAATGCTACACCAGGTCTTGGAAGATACCCTCCTTTCAAGAGGGAGGTTGTGGCCATTGCAAGTACTGCATTGGATGCGTTTAAGAATGAATCAAAGAAAATGGTGGTTGCACTGGTAGATATGGAGCGTGCTTTTGTGCCTCCTCAACACTTCATTCGCTTGGTGCAAAGGAGGATGGAGAGGCAGCGTAGAGAGGAAGAAGTGAAAAATCGATCTTCAAAGAAAGGACAAGAGGCAGAGCAAGCAATAATGAATCGGGCAACTAGCCCTCAAACAGGAGGTGAACAAGCTGGTGGTAGCATGAAATCAATGAAGGATAAGGATAAATCTGGTAAGGACGGCAAAGATGTGCAGGAGGCAACTGGTTTGAAGACTGCAGGTCCTGATGGCGAAATAACAGCAGGTTTCTTGTATAAAAGAAGTGCCAAAACTAATGGGTGGAGCAAGCGATGGTTTGTTTTGAATGAAAAGACTGGAAAGCTTGGCTACACCAAAAAGCAAGAGGAGAGACACTTTCGTGGTGTCATCACTTTGGAGGAGTGTAATATTGAAGAGGCTTCAGATGAGGATGAACCACCTCCATCAAAGAGTTCAAGGGACAAAAAGTCAAATGGACCAGAAAAATCACCCAGCCTTGTGTTTAAGATAACTAGTAAAGTTCCATATAAAACTGTTCTGAAAGCACACAGCGCCGTTGTGCTAAAGGCAGAGACTGTGGCAGACAAGCTAGAGTGGATAAACAAGATTGGAAAGGTTATTCAACCTTCTAAAGGACTACCCAGGGGTGCATCAACTGAAGGTGGTCCTACAATGCGACAAAGTCTTTCTGATGGTTCTCTTGATACAATGACTCGAAGACCTGCTGATCCTGAGGAAGAACTCCGGTGGATGTCTCAAGAAGTTCGGGGTTATGTTGAAGCTGTTTTGAATAGTTTGGCTGCTAATGTCCCTAAGGCGGTTGTTCTTTGCCAAGTTGAAAAGGCGAAGGAAGACATGTTAAATCAGCTATATAGCTCTGTCAGTGCTCAAAGCACAGCAAAGATTGAAGAGCTGCTTCAAGAGGATCAGAATGTGAAGGGTAGGAGGGAACGCTACCAGAAACAGTCACTACTCCTTTCCAAACTAACACGCCAACTCAGCATTCATGACAACCGAGCAGCTGCTGCATCCAGCTATTCTGTTGGTGGTGAGTATTGGACTAAAGCATGTCAAGATCTTCGTCCAGTGGTGCATCAGGTGATGACTGGAGATCCGCCTTTGATGCCGCCGCAAATGGTCCAGTTGACCGTAGTTCTTCATTATCTAGATCTTCGTCTAATGGTCACAACCGACATTATAGTGATCCAGCACAAAATGGTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000266 GO:0000902 GO:0000904 GO:0001101 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006892 GO:0006896 GO:0006897 GO:0006898 GO:0006996 GO:0007005 GO:0007034 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0009504 GO:0009506 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0012505 GO:0015631 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0021700 GO:0022622 GO:0030054 GO:0030135 GO:0030136 GO:0030139 GO:0030154 GO:0030276 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032878 GO:0032989 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045334 GO:0046907 GO:0048193 GO:0048285 GO:0048364 GO:0048468 GO:0048469 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055044 GO:0065007 GO:0071695 GO:0071840 GO:0071944 GO:0072583 GO:0090558 GO:0090627 GO:0097305 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0099402 GO:1901700 GO:1905392 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko04721 ko04961 ko05100 map04072 map04144 map04721 map04961 map05100
Pfam Domains
Protein Families
Dynamin_M Dynamin_N GED PH

Protein Analysis

899

Amino Acids

98.27

Weight (kDa)

8.9

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_N PF00350 42 - 205 3.5e-30 Dynamin family
Dynamin_M PF01031 257 - 494 6.8e-24 Dynamin central region
PH PF00169 578 - 697 1.1e-10 PH domain
PH_8 PF15409 580 - 631 2.9e-06 Pleckstrin homology domain
GED PF02212 736 - 818 9.4e-13 Dynamin GTPase effector domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10290 AT1G59610
fragaria_vesca FvH4_2g00460 FvH4_2g00460
malus_domestica MD05G1001600.v1.1 MD10G1002800.v1.1
prunus_persica Prupe.8G004400_v2.0.a1
pyrus_communis pycom05g00170 pycom10g00220
rosa_chinensis RchiOBHm_Chr6g0250411
rosa_laevigata RLG00000015541
rosa_multiflora Rmu_sc0001209.1_g000036
rosa_roxburghii Rroxscaffold_7G00217250
rosa_rugosa Rorug05G0496400 Rorug05G0496400
rosa_samantha Rh6AG005800 Rh6DG003800
rosa_wichuraiana Rw6G000640 Rw6G000710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 512
Acc36I ACCTGC 4 cut(s) 526, 1700, 1724, 2200
Acc65I GGTACC 1 cut(s) 587
AccB1I GGYRCC 3 cut(s) 50, 587, 998
AccB7I CCANNNNNTGG 1 cut(s) 1349
AciI CCGC 7 cut(s) 43, 54, 149, 2288, 2585, 2597, 2600
AclWI GGATC 5 cut(s) 458, 2192, 2403, 2576, 2668
AcoI YGGCCR 1 cut(s) 1383
AcsI RAATTY 1 cut(s) 1108
AcuI CTGAAG 4 cut(s) 615, 827, 1875, 2148
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 5 cut(s) 403, 575, 589, 1296, 1396
AfiI CCNNNNNNNGG 9 cut(s) 102, 1222, 1223, 1224, 1306, 1349, 1607, 2102, 2287
AflIII ACRYGT 1 cut(s) 2325
AgeI ACCGGT 1 cut(s) 200
AhdI GACNNNNNGTC 2 cut(s) 1710, 1936
AhlI ACTAGT 1 cut(s) 1982
AjiI CACGTC 1 cut(s) 1277
AjnI CCWGG 6 cut(s) 433, 657, 782, 1305, 1342, 2111
AjuI GAANNNNNNNTTGG 4 cut(s) 901, 933, 2457, 2489
AleI CACNNNNGTG 1 cut(s) 1848
AloI GAACNNNNNNTCC 2 cut(s) 77, 109
Alw21I GWGCWC 4 cut(s) 226, 489, 829, 2359
Alw26I GTCTC 5 cut(s) 942, 1283, 1834, 2036, 2229
AlwI GGATC 5 cut(s) 458, 2192, 2403, 2576, 2668
AlwNI CAGNNNCTG 3 cut(s) 1715, 2045, 2495
Ama87I CYCGRG 3 cut(s) 219, 369, 549
AoxI GGCC 2 cut(s) 680, 1383
ApoI RAATTY 1 cut(s) 1108
ArsI GACNNNNNNTTYG 2 cut(s) 1133, 1165
AseI ATTAAT 1 cut(s) 381
AsiGI ACCGGT 1 cut(s) 200
Asp718I GGTACC 1 cut(s) 587
AspLEI GCGC 2 cut(s) 53, 2024
AspS9I GGNCC 5 cut(s) 680, 1712, 1946, 2135, 2608
AsuHPI GGTGA 6 cut(s) 215, 1079, 1625, 1950, 2531, 2581
AvaI CYCGRG 3 cut(s) 219, 369, 549
AvaII GGWCC 4 cut(s) 1712, 1946, 2135, 2608
AxyI CCTNAGG 2 cut(s) 2202, 2283
BaeGI GKGCMC 1 cut(s) 1001
BalI TGGCCA 1 cut(s) 1385
BanI GGYRCC 3 cut(s) 50, 587, 998
BanII GRGCYC 3 cut(s) 226, 556, 829
BbsI GAAGAC 3 cut(s) 1709, 2194, 2328
Bbv12I GWGCWC 4 cut(s) 226, 489, 829, 2359
BbvCI CCTCAGC 1 cut(s) 993
BceAI ACGGC 5 cut(s) 341, 873, 1011, 1687, 2009
BciT130I CCWGG 6 cut(s) 435, 659, 784, 1307, 1344, 2113
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 5 cut(s) 942, 1283, 1834, 2036, 2229
BcuI ACTAGT 1 cut(s) 1982
BfaI CTAG 6 cut(s) 773, 1010, 1596, 1983, 2060, 2636
BfmI CTRYAG 4 cut(s) 281, 357, 651, 1707
BfoI RGCGCY 2 cut(s) 54, 2025
BfuAI ACCTGC 4 cut(s) 526, 1700, 1724, 2200
BglI GCCNNNNNGGC 1 cut(s) 788
BglII AGATCT 4 cut(s) 1004, 1232, 2545, 2638
BlpI GCTNAGC 1 cut(s) 483
BmcAI AGTACT 1 cut(s) 1396
Bme1390I CCNGG 6 cut(s) 435, 659, 784, 1307, 1344, 2113
Bme18I GGWCC 4 cut(s) 1712, 1946, 2135, 2608
BmeRI GACNNNNNGTC 2 cut(s) 1710, 1936
BmeT110I CYCGRG 3 cut(s) 219, 369, 549
BmgBI CACGTC 1 cut(s) 1277
BmgT120I GGNCC 5 cut(s) 680, 1712, 1946, 2135, 2608
BmiI GGNNCC 3 cut(s) 52, 589, 1000
BmrFI CCNGG 6 cut(s) 435, 659, 784, 1307, 1344, 2113
BpiI GAAGAC 3 cut(s) 1709, 2194, 2328
BpmI CTGGAG 3 cut(s) 774, 805, 2598
Bpu10I CCTNAGC 1 cut(s) 993
Bpu1102I GCTNAGC 1 cut(s) 483
BpuEI CTTGAG 2 cut(s) 510, 2211
Bsa29I ATCGAT 1 cut(s) 1543
BsaBI GATNNNNATC 1 cut(s) 1194
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 5 cut(s) 434, 704, 855, 2111, 2112
BsaWI WCCGGW 4 cut(s) 200, 532, 821, 2214
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 208, 238, 811, 841
Bsc4I CCNNNNNNNGG 9 cut(s) 102, 1222, 1223, 1224, 1306, 1349, 1607, 2102, 2287
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 7 cut(s) 111, 1449, 1699, 1810, 2555, 2581, 2611
Bse21I CCTNAGG 2 cut(s) 2202, 2283
Bse3DI GCAATG 2 cut(s) 145, 1386
Bse8I GATNNNNATC 1 cut(s) 1194
BseBI CCWGG 6 cut(s) 435, 659, 784, 1307, 1344, 2113
BseCI ATCGAT 1 cut(s) 1543
BseDI CCNNGG 5 cut(s) 434, 704, 855, 2111, 2112
BseJI GATNNNNATC 1 cut(s) 1194
BseLI CCNNNNNNNGG 9 cut(s) 102, 1222, 1223, 1224, 1306, 1349, 1607, 2102, 2287
BseMI GCAATG 2 cut(s) 145, 1386
BseMII CTCAG 5 cut(s) 474, 571, 984, 2193, 2482
BseNI ACTGG 7 cut(s) 111, 1449, 1699, 1810, 2555, 2581, 2611
BseRI GAGGAG 5 cut(s) 29, 85, 1464, 1850, 1883
BseSI GKGCMC 1 cut(s) 1001
BseYI CCCAGC 2 cut(s) 346, 1961
BsgI GTGCAG 3 cut(s) 729, 1004, 1703
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 682, 1385
BshNI GGYRCC 3 cut(s) 50, 587, 998
BshTI ACCGGT 1 cut(s) 200
BshVI ATCGAT 1 cut(s) 1543
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 4 cut(s) 226, 489, 829, 2359
BsiHKCI CYCGRG 3 cut(s) 219, 369, 549
BsiSI CCGG 4 cut(s) 201, 533, 822, 2215
BslFI GGGAC 2 cut(s) 1943, 2264
BslI CCNNNNNNNGG 9 cut(s) 102, 1222, 1223, 1224, 1306, 1349, 1607, 2102, 2287
BsmAI GTCTC 5 cut(s) 942, 1283, 1834, 2036, 2229
BsmBI CGTCTC 1 cut(s) 1283
BsmFI GGGAC 2 cut(s) 1943, 2264
BsmI GAATGC 2 cut(s) 328, 2472
BsnI GGCC 2 cut(s) 682, 1385
BsoBI CYCGRG 3 cut(s) 219, 369, 549
Bsp1286I GDGCHC 6 cut(s) 226, 489, 556, 829, 1001, 2359
Bsp1720I GCTNAGC 1 cut(s) 483
BspACI CCGC 7 cut(s) 43, 54, 149, 2288, 2585, 2597, 2600
BspANI GGCC 2 cut(s) 682, 1385
BspCNI CTCAG 5 cut(s) 475, 570, 985, 2194, 2481
BspDI ATCGAT 1 cut(s) 1543
BspHI TCATGA 1 cut(s) 2476
BspLI GGNNCC 3 cut(s) 52, 589, 1000
BspMAI CTGCAG 3 cut(s) 285, 655, 1711
BspMI ACCTGC 4 cut(s) 526, 1700, 1724, 2200
BspPI GGATC 5 cut(s) 458, 2192, 2403, 2576, 2668
BspQI GCTCTTC 1 cut(s) 2373
BspT107I GGYRCC 3 cut(s) 50, 587, 998
BsrDI GCAATG 2 cut(s) 145, 1386
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 7 cut(s) 111, 1449, 1699, 1810, 2555, 2581, 2611
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 5 cut(s) 434, 704, 855, 2111, 2112
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 2 cut(s) 704, 855
Bst2UI CCWGG 6 cut(s) 435, 659, 784, 1307, 1344, 2113
Bst4CI ACNGT 8 cut(s) 173, 598, 750, 1301, 2005, 2047, 2436, 2620
Bst6I CTCTTC 3 cut(s) 1163, 1878, 2373
BstACI GRCGYC 1 cut(s) 51
BstAPI GCANNNNNTGC 4 cut(s) 334, 1397, 1406, 2495
BstC8I GCNNGC 4 cut(s) 1260, 1461, 1575, 1778
BstDEI CTNAG 7 cut(s) 483, 557, 993, 1252, 2202, 2283, 2468
BstENI CCTNNNNNAGG 3 cut(s) 100, 1605, 2100
BstH2I RGCGCY 2 cut(s) 54, 2025
BstHHI GCGC 2 cut(s) 53, 2024
BstMAI GTCTC 5 cut(s) 942, 1283, 1834, 2036, 2229
BstMCI CGRYCG 1 cut(s) 12
BstNI CCWGG 6 cut(s) 435, 659, 784, 1307, 1344, 2113
BstNSI RCATGY 2 cut(s) 2329, 2541
BstSCI CCNGG 6 cut(s) 433, 657, 782, 1305, 1342, 2111
BstSFI CTRYAG 4 cut(s) 281, 357, 651, 1707
BstSLI GKGCMC 1 cut(s) 1001
BstV2I GAAGAC 3 cut(s) 1709, 2194, 2328
BstX2I RGATCY 5 cut(s) 1004, 1232, 2545, 2581, 2638
BstYI RGATCY 5 cut(s) 1004, 1232, 2545, 2581, 2638
Bsu15I ATCGAT 1 cut(s) 1543
Bsu36I CCTNAGG 2 cut(s) 2202, 2283
BsuRI GGCC 2 cut(s) 682, 1385
BsuTUI ATCGAT 1 cut(s) 1543
BtgZI GCGATG 1 cut(s) 1795
BtrI CACGTC 1 cut(s) 1277
BtsI GCAGTG 1 cut(s) 332
BtsIMutI CAGTG 4 cut(s) 332, 1442, 2359, 2562
BveI ACCTGC 4 cut(s) 526, 1700, 1724, 2200
Cac8I GCNNGC 4 cut(s) 1260, 1461, 1575, 1778
CaiI CAGNNNCTG 3 cut(s) 1715, 2045, 2495
CciI TCATGA 1 cut(s) 2476
CfoI GCGC 2 cut(s) 53, 2024
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 5 cut(s) 680, 1712, 1946, 2135, 2608
ClaI ATCGAT 1 cut(s) 1543
CseI GACGC 1 cut(s) 963
CsiI ACCWGGT 1 cut(s) 1342
Csp6I GTAC 5 cut(s) 402, 574, 588, 1295, 1395
CspAI ACCGGT 1 cut(s) 200
CviAII CATG 5 cut(s) 454, 1633, 2326, 2477, 2538
CviQI GTAC 5 cut(s) 402, 574, 588, 1295, 1395
DdeI CTNAG 7 cut(s) 483, 557, 993, 1252, 2202, 2283, 2468
DinI GGCGCC 1 cut(s) 52
DriI GACNNNNNGTC 2 cut(s) 1710, 1936
EaeI YGGCCR 1 cut(s) 1383
Eam1104I CTCTTC 3 cut(s) 1163, 1878, 2373
Eam1105I GACNNNNNGTC 2 cut(s) 1710, 1936
EarI CTCTTC 3 cut(s) 1163, 1878, 2373
EciI GGCGGA 1 cut(s) 2574
Ecl136II GAGCTC 2 cut(s) 224, 827
Eco130I CCWWGG 2 cut(s) 704, 855
Eco24I GRGCYC 3 cut(s) 226, 556, 829
Eco32I GATATC 1 cut(s) 1156
Eco47I GGWCC 4 cut(s) 1712, 1946, 2135, 2608
Eco53kI GAGCTC 2 cut(s) 224, 827
Eco57I CTGAAG 4 cut(s) 615, 827, 1875, 2148
Eco81I CCTNAGG 2 cut(s) 2202, 2283
Eco88I CYCGRG 3 cut(s) 219, 369, 549
EcoICRI GAGCTC 2 cut(s) 224, 827
EcoNI CCTNNNNNAGG 3 cut(s) 100, 1605, 2100
EcoO109I RGGNCCY 1 cut(s) 1712
EcoRII CCWGG 6 cut(s) 433, 657, 782, 1305, 1342, 2111
EcoRV GATATC 1 cut(s) 1156
EcoT14I CCWWGG 2 cut(s) 704, 855
EcoT38I GRGCYC 3 cut(s) 226, 556, 829
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 2 cut(s) 704, 855
Esp3I CGTCTC 1 cut(s) 1283
FaeI CATG 5 cut(s) 457, 1636, 2329, 2480, 2541
FalI AAGNNNNNCTT 4 cut(s) 888, 920, 2370, 2402
FaqI GGGAC 2 cut(s) 1943, 2264
FatI CATG 5 cut(s) 453, 1632, 2325, 2476, 2537
FauI CCCGC 1 cut(s) 142
FbaI TGATCA 1 cut(s) 442
FriOI GRGCYC 3 cut(s) 226, 556, 829
FspBI CTAG 6 cut(s) 773, 1010, 1596, 1983, 2060, 2636
GlaI GCGC 2 cut(s) 52, 2023
GsaI CCCAGC 2 cut(s) 350, 1965
GsuI CTGGAG 3 cut(s) 774, 805, 2598
HaeII RGCGCY 2 cut(s) 54, 2025
HaeIII GGCC 2 cut(s) 682, 1385
HapII CCGG 4 cut(s) 201, 533, 822, 2215
HgaI GACGC 1 cut(s) 963
HhaI GCGC 2 cut(s) 53, 2024
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 5 cut(s) 457, 1636, 2329, 2480, 2541
Hin6I GCGC 2 cut(s) 51, 2022
HinP1I GCGC 2 cut(s) 51, 2022
HincII GTYRAC 2 cut(s) 79, 2616
HindII GTYRAC 2 cut(s) 79, 2616
HindIII AAGCTT 3 cut(s) 839, 965, 1811
HinfI GANTC 7 cut(s) 35, 461, 494, 880, 1421, 1585, 2182
HpaII CCGG 4 cut(s) 201, 533, 822, 2215
HphI GGTGA 6 cut(s) 215, 1079, 1625, 1950, 2531, 2581
Hpy166II GTNNAC 4 cut(s) 79, 1297, 1616, 2616
Hpy8I GTNNAC 4 cut(s) 79, 1297, 1616, 2616
Hpy99I CGWCG 3 cut(s) 71, 128, 131
HpyAV CCTTC 6 cut(s) 1097, 1639, 2106, 2123, 2311, 2401
HpyCH4III ACNGT 8 cut(s) 173, 598, 750, 1301, 2005, 2047, 2436, 2620
HpyCH4IV ACGT 2 cut(s) 123, 1276
HpyF3I CTNAG 7 cut(s) 483, 557, 993, 1252, 2202, 2283, 2468
HpySE526I ACGT 2 cut(s) 123, 1276
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 5 cut(s) 457, 1636, 2329, 2480, 2541
HspAI GCGC 2 cut(s) 51, 2022
KasI GGCGCC 1 cut(s) 50
KpnI GGTACC 1 cut(s) 591
Ksp22I TGATCA 1 cut(s) 442
LguI GCTCTTC 1 cut(s) 2373
LmnI GCTCC 6 cut(s) 229, 824, 832, 1018, 1456, 1773
MabI ACCWGGT 1 cut(s) 1342
MaeI CTAG 6 cut(s) 773, 1010, 1596, 1983, 2060, 2636
MaeII ACGT 2 cut(s) 123, 1276
MaeIII GTNAC 4 cut(s) 350, 924, 2436, 2653
MflI RGATCY 5 cut(s) 1004, 1232, 2545, 2581, 2638
MhlI GDGCHC 6 cut(s) 226, 489, 556, 829, 1001, 2359
MlsI TGGCCA 1 cut(s) 1385
MluCI AATT 7 cut(s) 378, 382, 419, 498, 956, 1086, 1108
MluNI TGGCCA 1 cut(s) 1385
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 3 cut(s) 29, 470, 2176
MmeI TCCRAC 2 cut(s) 1075, 1161
Mox20I TGGCCA 1 cut(s) 1385
MscI TGGCCA 1 cut(s) 1385
MseI TTAA 7 cut(s) 120, 381, 671, 1238, 1413, 1974, 2330
MslI CAYNNNNRTG 2 cut(s) 1848, 2669
Msp20I TGGCCA 1 cut(s) 1385
MspA1I CMGCKG 1 cut(s) 2492
MspI CCGG 4 cut(s) 201, 533, 822, 2215
MspR9I CCNGG 6 cut(s) 435, 659, 784, 1307, 1344, 2113
Mva1269I GAATGC 2 cut(s) 328, 2472
MvaI CCWGG 6 cut(s) 435, 659, 784, 1307, 1344, 2113
NarI GGCGCC 1 cut(s) 51
NlaIII CATG 5 cut(s) 457, 1636, 2329, 2480, 2541
NlaIV GGNNCC 3 cut(s) 52, 589, 1000
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 2 cut(s) 2436, 2653
NspI RCATGY 2 cut(s) 2329, 2541
OliI CACNNNNGTG 1 cut(s) 1848
PaeR7I CTCGAG 2 cut(s) 219, 549
PagI TCATGA 1 cut(s) 2476
PasI CCCWGGG 1 cut(s) 2112
PciI ACATGT 1 cut(s) 2325
PciSI GCTCTTC 1 cut(s) 2373
PctI GAATGC 2 cut(s) 328, 2472
PfeI GAWTC 4 cut(s) 494, 880, 1421, 1585
PflFI GACNNNGTC 1 cut(s) 2151
PflMI CCANNNNNTGG 1 cut(s) 1349
PinAI ACCGGT 1 cut(s) 200
Ple19I CGATCG 1 cut(s) 12
PleI GAGTC 3 cut(s) 29, 469, 2176
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 3 cut(s) 29, 469, 2176
PpuMI RGGWCCY 1 cut(s) 1712
PscI ACATGT 1 cut(s) 2325
PshBI ATTAAT 1 cut(s) 381
PsiI TTATAA 1 cut(s) 512
Psp124BI GAGCTC 2 cut(s) 226, 829
Psp5II RGGWCCY 1 cut(s) 1712
Psp6I CCWGG 6 cut(s) 433, 657, 782, 1305, 1342, 2111
PspFI CCCAGC 2 cut(s) 346, 1961
PspGI CCWGG 6 cut(s) 433, 657, 782, 1305, 1342, 2111
PspN4I GGNNCC 3 cut(s) 52, 589, 1000
PspPI GGNCC 5 cut(s) 680, 1712, 1946, 2135, 2608
PspPPI RGGWCCY 1 cut(s) 1712
PspXI VCTCGAGB 1 cut(s) 219
PstI CTGCAG 3 cut(s) 285, 655, 1711
PstNI CAGNNNCTG 3 cut(s) 1715, 2045, 2495
PsuI RGATCY 5 cut(s) 1004, 1232, 2545, 2581, 2638
PsyI GACNNNGTC 1 cut(s) 2151
PvuI CGATCG 1 cut(s) 12
PvuII CAGCTG 1 cut(s) 2492
RsaI GTAC 5 cut(s) 403, 575, 589, 1296, 1396
RsaNI GTAC 5 cut(s) 402, 574, 588, 1295, 1395
RseI CAYNNNNRTG 2 cut(s) 1848, 2669
SacI GAGCTC 2 cut(s) 226, 829
SapI GCTCTTC 1 cut(s) 2373
SaqAI TTAA 7 cut(s) 120, 381, 671, 1238, 1413, 1974, 2330
Sau96I GGNCC 5 cut(s) 680, 1712, 1946, 2135, 2608
ScaI AGTACT 1 cut(s) 1396
SchI GAGTC 3 cut(s) 29, 470, 2176
ScrFI CCNGG 6 cut(s) 435, 659, 784, 1307, 1344, 2113
SduI GDGCHC 6 cut(s) 226, 489, 556, 829, 1001, 2359
SexAI ACCWGGT 1 cut(s) 1342
SfcI CTRYAG 4 cut(s) 281, 357, 651, 1707
SfoI GGCGCC 1 cut(s) 52
Sfr274I CTCGAG 2 cut(s) 219, 549
SinI GGWCC 4 cut(s) 1712, 1946, 2135, 2608
SlaI CTCGAG 2 cut(s) 219, 549
SmiMI CAYNNNNRTG 2 cut(s) 1848, 2669
SmlI CTYRAG 4 cut(s) 219, 525, 549, 2226
SmoI CTYRAG 4 cut(s) 219, 525, 549, 2226
SpeI ACTAGT 1 cut(s) 1982
Sse9I AATT 7 cut(s) 378, 382, 419, 498, 956, 1086, 1108
SsiI CCGC 7 cut(s) 43, 54, 149, 2288, 2585, 2597, 2600
SspDI GGCGCC 1 cut(s) 50
SspI AATATT 1 cut(s) 1879
SspMI CTAG 6 cut(s) 773, 1010, 1596, 1983, 2060, 2636
SstI GAGCTC 2 cut(s) 226, 829
StyD4I CCNGG 6 cut(s) 433, 657, 782, 1305, 1342, 2111
StyI CCWWGG 2 cut(s) 704, 855
TaaI ACNGT 8 cut(s) 173, 598, 750, 1301, 2005, 2047, 2436, 2620
TaiI ACGT 2 cut(s) 126, 1279
TaqI TCGA 7 cut(s) 12, 38, 220, 550, 901, 1543, 2185
TaqII GACCGA 1 cut(s) 1158
TasI AATT 7 cut(s) 378, 382, 419, 498, 956, 1086, 1108
TatI WGTACW 2 cut(s) 401, 1394
TauI GCSGC 4 cut(s) 46, 56, 2599, 2602
TfiI GAWTC 4 cut(s) 494, 880, 1421, 1585
Tru1I TTAA 7 cut(s) 120, 381, 671, 1238, 1413, 1974, 2330
Tru9I TTAA 7 cut(s) 120, 381, 671, 1238, 1413, 1974, 2330
TscAI CASTG 4 cut(s) 339, 1449, 2359, 2562
TseFI GTSAC 2 cut(s) 2436, 2653
Tsp45I GTSAC 2 cut(s) 2436, 2653
TspGWI ACGGA 2 cut(s) 409, 463
TspRI CASTG 4 cut(s) 339, 1449, 2359, 2562
Tth111I GACNNNGTC 1 cut(s) 2151
Van91I CCANNNNNTGG 1 cut(s) 1349
VpaK11BI GGWCC 4 cut(s) 1712, 1946, 2135, 2608
VspI ATTAAT 1 cut(s) 381
XagI CCTNNNNNAGG 3 cut(s) 100, 1605, 2100
XapI RAATTY 1 cut(s) 1108
XbaI TCTAGA 1 cut(s) 2635
XceI RCATGY 2 cut(s) 2329, 2541
XcmI CCANNNNNNNNNTGG 1 cut(s) 839
XhoI CTCGAG 2 cut(s) 219, 549
XspI CTAG 6 cut(s) 773, 1010, 1596, 1983, 2060, 2636
ZrmI AGTACT 1 cut(s) 1396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.