MD05G1038500.v1.1

Exostosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014147
Physical Location & Seq
Reverse (-)
6522572 .. 6524984
2413 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1038500.v1.1.491

Sequence Viewer

Length: 1464 bp
ATGGCAGTGAAGGGCACCGCCACAACGACACCTTCTTCATCCGTTTGCTCTGTTCCCACTCTCTTCTTCGCATTCACTCTCCTCTGCGCTCTCTCTCTTTCTCTCTTCTTCCTCTCCAATCCTTCTCCTTCTTCCTCTCCTTCTCCTTCTTCCTCATCTTTCATTTCCCTAAACGCCCCTCTCTCTCCCCAAAATTCCCTCAAAGTCTACCTAGCAGACCTCCCCAGATCCCTCAACTATGGCCTCCTCGAAAAGTACTGGGCTTCCGGCCCCGATTCCCGCCTCCAAACGGACACCGACCACCAAATCCCAGACTCCCATTTGCCCAAAAACCTTGAATTTCCACCCTATCCAGATAATCCATTGATTAAACAGTACAGCGCCGAGTACTGGATTTTGGGGGACCTGATGACCCCTCCGGAGCACAGAACTGGGTCGTTTGCCTCCCGGGTTTTCGATGCCGACGAAGCCGACGTTGTTTTCGTCCCCTTTTTCGCGACCTTGAGCGCAGAATTGCAGCTGGCTACGGCTAAAGGGGTGTTTAGGAAGAAGGCTGGGAATGAGGACTATGAGAGGCAGAGGCAAGTGGTGGACTTTGTGAAGAACACTGAGGCTTGGAAAAGGTCAGGTGGAAGAGACCATGTGTTTGTGCTTACTGACCCAGTGGCAATGTGGCACGTTCGAGCAGAGATAGCCCCGGCGGTCCTTCTCGTTGTAGATTTTGGAGGGTGGTACACACTCGAATCCAGGTCATCAAACGGTAACTCATCAGATGTGATTCAGCACACACAAGTGTCACTACTCAAAGATGTCATTGTGCCCTACACCCATCTACTTCCCCGATTGCAACTGACAGAAAATAAGAAGCGTCAAACGCTACTTTACTTTAAAGGAGCTAAACATAGGCACCGGGGAGGCTTAGTTCGAGAAACATTGTGGGACTTGTTGGTTAATGAGCCAGATGTTATCATGGAAGAAGGCTTCCCTAACGCCACTGGGAGGGAACAGTCCATAAAAGGGATGAGAACTTCAGAGTTTTGCTTGCATCCAGCTGGGGACACTCCCACTTCATGTCGGCTGTTCGATGCAATGCAAAGTCTCTGTATACCCGTTATTGTCAGTGACAACATAGAACTTCCTTTCGAAGGGATGGTAGATTATTCAGAGTTTTCTGTTTTTGTGGCAGTCGATGATGCGTTGAAACCGAACTGGATTGTGAGCCATCTTAGGACCTTTACTAGAGAGCAGAGGGATAGATTTCGTCGAACAATGGCACAGTTTCAGCCCATGTTTGAGTATGACAATGGTCATCCTGGTGGCATCGGGCCAATTCCTCCCGACGGTGCAGTGAACCACATGTGGAAGATGGTTCATCAAAAGTTGCCAATGATCAAGGAATCAATCGTTCGTGAAAGGAGAAAACCTCCCGGTGTTTCAGTTCCACGCCGTTGCCACTGTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

488

Amino Acids

54.74

Weight (kDa)

6.74

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 64 - 408 3.2e-70 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34270
fragaria_vesca FvH4_2g12430
malus_domestica MD05G1038500.v1.1 MD10G1044300.v1.1
prunus_persica Prupe.8G050800_v2.0.a1
pyrus_communis pycom05g03140 pycom10g03090
rosa_chinensis RchiOBHm_Chr6g0272591
rosa_laevigata RLG00000013663
rosa_multiflora Rmu_sc0003080.1_g000005
rosa_roxburghii Rroxscaffold_7G00196090
rosa_rugosa Rorug06G0070100
rosa_samantha Rh6AG186500 Rh6BG189800 Rh6CG187600 Rh6DG179500
rosa_wichuraiana Rw6G016080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 14, 906
AccI GTMKAC 2 cut(s) 207, 1105
AccII CGCG 1 cut(s) 497
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 3 cut(s) 18, 280, 701
AclWI GGATC 1 cut(s) 222
AcsI RAATTY 2 cut(s) 193, 338
AcuI CTGAAG 1 cut(s) 1014
AfaI GTAC 5 cut(s) 257, 377, 389, 734, 1459
AfiI CCNNNNNNNGG 6 cut(s) 289, 390, 999, 1017, 1145, 1340
AflIII ACRYGT 1 cut(s) 1356
AgsI TTSAA 2 cut(s) 338, 1201
AjnI CCWGG 2 cut(s) 746, 1312
AleI CACNNNNGTG 1 cut(s) 791
AloI GAACNNNNNNTCC 2 cut(s) 906, 938
AluBI AGCT 3 cut(s) 520, 896, 1052
AluI AGCT 3 cut(s) 520, 896, 1052
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 2 cut(s) 630, 1103
AlwI GGATC 1 cut(s) 222
Ama87I CYCGRG 1 cut(s) 447
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 3 cut(s) 241, 268, 1325
ApeKI GCWGC 1 cut(s) 517
ApoI RAATTY 2 cut(s) 193, 338
ArsI GACNNNNNNTTYG 4 cut(s) 464, 496, 629, 661
AspLEI GCGC 3 cut(s) 89, 383, 509
AspS9I GGNCC 5 cut(s) 269, 403, 703, 1230, 1325
AsuC2I CCSGG 5 cut(s) 448, 449, 698, 911, 1428
AsuII TTCGAA 1 cut(s) 1143
AvaI CYCGRG 1 cut(s) 447
AvaII GGWCC 3 cut(s) 403, 703, 1230
BaeGI GKGCMC 2 cut(s) 17, 822
BanI GGYRCC 2 cut(s) 14, 906
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 1 cut(s) 529
BccI CCATC 4 cut(s) 837, 1144, 1230, 1360
BceAI ACGGC 2 cut(s) 543, 1431
BciT130I CCWGG 2 cut(s) 748, 1314
BclI TGATCA 1 cut(s) 1389
BcnI CCSGG 5 cut(s) 448, 449, 698, 911, 1428
BcoDI GTCTC 2 cut(s) 630, 1103
BfaI CTAG 2 cut(s) 212, 1239
BfoI RGCGCY 1 cut(s) 384
BisI GCNGC 1 cut(s) 518
BlsI GCNGC 1 cut(s) 519
BmcAI AGTACT 2 cut(s) 257, 389
Bme1390I CCNGG 7 cut(s) 448, 449, 698, 748, 911, 1314, 1428
Bme18I GGWCC 3 cut(s) 403, 703, 1230
BmeT110I CYCGRG 1 cut(s) 447
BmgT120I GGNCC 5 cut(s) 269, 403, 703, 1230, 1325
BmiI GGNNCC 4 cut(s) 16, 271, 404, 908
BmrFI CCNGG 7 cut(s) 448, 449, 698, 748, 911, 1314, 1428
BmrI ACTGGG 4 cut(s) 268, 441, 656, 1005
BmsI GCATC 5 cut(s) 448, 1054, 1075, 1183, 1329
BmuI ACTGGG 4 cut(s) 268, 441, 656, 1005
BoxI GACNNNNGTC 1 cut(s) 1305
BplI GAGNNNNNCTC 2 cut(s) 1408, 1440
Bpu14I TTCGAA 1 cut(s) 1143
BpuEI CTTGAG 1 cut(s) 523
BpuMI CCSGG 5 cut(s) 448, 449, 698, 911, 1428
BsaAI YACGTR 1 cut(s) 1461
BsaI GGTCTC 1 cut(s) 630
BsaJI CCNNGG 3 cut(s) 447, 696, 910
BsaWI WCCGGW 1 cut(s) 418
BsaXI ACNNNNNCTCC 2 cut(s) 906, 936
Bsc4I CCNNNNNNNGG 6 cut(s) 289, 390, 999, 1017, 1145, 1340
Bse1I ACTGG 6 cut(s) 263, 395, 436, 662, 1000, 1214
Bse3DI GCAATG 2 cut(s) 675, 1095
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 2 cut(s) 748, 1314
BseDI CCNNGG 3 cut(s) 447, 696, 910
BseGI GGATG 5 cut(s) 38, 1026, 1045, 1155, 1309
BseLI CCNNNNNNNGG 6 cut(s) 289, 390, 999, 1017, 1145, 1340
BseMI GCAATG 2 cut(s) 675, 1095
BseMII CTCAG 1 cut(s) 600
BseNI ACTGG 6 cut(s) 263, 395, 436, 662, 1000, 1214
BseRI GAGGAG 2 cut(s) 71, 236
BseSI GKGCMC 2 cut(s) 17, 822
BseXI GCAGC 1 cut(s) 529
BseYI CCCAGC 2 cut(s) 554, 1052
BsgI GTGCAG 1 cut(s) 1365
Bsh1236I CGCG 1 cut(s) 497
BshFI GGCC 3 cut(s) 243, 270, 1327
BshNI GGYRCC 2 cut(s) 14, 906
BsiHKAI GWGCWC 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 6 cut(s) 267, 419, 448, 698, 910, 1428
BslFI GGGAC 4 cut(s) 416, 470, 953, 1070
BslI CCNNNNNNNGG 6 cut(s) 289, 390, 999, 1017, 1145, 1340
BsmAI GTCTC 2 cut(s) 630, 1103
BsmFI GGGAC 4 cut(s) 416, 470, 953, 1070
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 3 cut(s) 243, 270, 1327
Bso31I GGTCTC 1 cut(s) 630
BsoBI CYCGRG 1 cut(s) 447
Bsp119I TTCGAA 1 cut(s) 1143
Bsp1286I GDGCHC 3 cut(s) 17, 426, 822
Bsp13I TCCGGA 1 cut(s) 418
Bsp143I GATC 2 cut(s) 227, 1389
Bsp68I TCGCGA 1 cut(s) 497
BspACI CCGC 3 cut(s) 18, 280, 701
BspANI GGCC 3 cut(s) 243, 270, 1327
BspCNI CTCAG 1 cut(s) 601
BspEI TCCGGA 1 cut(s) 418
BspFNI CGCG 1 cut(s) 497
BspLI GGNNCC 4 cut(s) 16, 271, 404, 908
BspPI GGATC 1 cut(s) 222
BspT104I TTCGAA 1 cut(s) 1143
BspT107I GGYRCC 2 cut(s) 14, 906
BspTNI GGTCTC 1 cut(s) 630
BsrDI GCAATG 2 cut(s) 675, 1095
BsrI ACTGG 6 cut(s) 263, 395, 436, 662, 1000, 1214
BssECI CCNNGG 3 cut(s) 447, 696, 910
BssMI GATC 2 cut(s) 227, 1389
BssNAI GTATAC 1 cut(s) 1106
Bst1107I GTATAC 1 cut(s) 1106
Bst2UI CCWGG 2 cut(s) 748, 1314
Bst4CI ACNGT 6 cut(s) 375, 761, 1008, 1278, 1343, 1457
Bst6I CTCTTC 3 cut(s) 68, 110, 628
BstBAI YACGTR 1 cut(s) 1461
BstBI TTCGAA 1 cut(s) 1143
BstC8I GCNNGC 2 cut(s) 522, 1043
BstDEI CTNAG 3 cut(s) 609, 919, 1226
BstENI CCTNNNNNAGG 1 cut(s) 1143
BstF5I GGATG 5 cut(s) 38, 1026, 1045, 1155, 1309
BstFNI CGCG 1 cut(s) 497
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 3 cut(s) 89, 383, 509
BstKTI GATC 2 cut(s) 230, 1392
BstMAI GTCTC 2 cut(s) 630, 1103
BstMBI GATC 2 cut(s) 227, 1389
BstMWI GCNNNNNNNGC 3 cut(s) 467, 692, 874
BstNI CCWGG 2 cut(s) 748, 1314
BstNSI RCATGY 1 cut(s) 1360
BstPAI GACNNNNGTC 1 cut(s) 1305
BstSCI CCNGG 7 cut(s) 446, 447, 696, 746, 909, 1312, 1426
BstSLI GKGCMC 2 cut(s) 17, 822
BstUI CGCG 1 cut(s) 497
BstV1I GCAGC 1 cut(s) 529
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
BstZ17I GTATAC 1 cut(s) 1106
BsuRI GGCC 3 cut(s) 243, 270, 1327
BtsCI GGATG 5 cut(s) 38, 1026, 1045, 1155, 1309
BtsI GCAGTG 2 cut(s) 12, 1353
BtsIMutI CAGTG 7 cut(s) 12, 606, 669, 993, 1126, 1353, 1453
BtuMI TCGCGA 1 cut(s) 497
Cac8I GCNNGC 2 cut(s) 522, 1043
CfoI GCGC 3 cut(s) 89, 383, 509
Cfr13I GGNCC 5 cut(s) 269, 403, 703, 1230, 1325
Cfr9I CCCGGG 1 cut(s) 447
CseI GACGC 1 cut(s) 857
Csp6I GTAC 5 cut(s) 256, 376, 388, 733, 1458
CviAII CATG 5 cut(s) 641, 970, 1071, 1288, 1357
CviQI GTAC 5 cut(s) 256, 376, 388, 733, 1458
DdeI CTNAG 3 cut(s) 609, 919, 1226
DpnI GATC 2 cut(s) 229, 1391
DpnII GATC 2 cut(s) 227, 1389
DraI TTTAAA 1 cut(s) 889
Eam1104I CTCTTC 3 cut(s) 68, 110, 628
EarI CTCTTC 3 cut(s) 68, 110, 628
Eco31I GGTCTC 1 cut(s) 630
Eco47I GGWCC 3 cut(s) 403, 703, 1230
Eco57I CTGAAG 1 cut(s) 1014
Eco88I CYCGRG 1 cut(s) 447
EcoNI CCTNNNNNAGG 1 cut(s) 1143
EcoO109I RGGNCCY 2 cut(s) 403, 1230
EcoRII CCWGG 2 cut(s) 746, 1312
FaeI CATG 5 cut(s) 644, 973, 1074, 1291, 1360
FaqI GGGAC 4 cut(s) 416, 470, 953, 1070
FatI CATG 5 cut(s) 640, 969, 1070, 1287, 1356
FauI CCCGC 1 cut(s) 287
FbaI TGATCA 1 cut(s) 1389
FblI GTMKAC 2 cut(s) 207, 1105
Fnu4HI GCNGC 1 cut(s) 518
FokI GGATG 5 cut(s) 25, 1032, 1033, 1162, 1296
Fsp4HI GCNGC 1 cut(s) 518
FspBI CTAG 2 cut(s) 212, 1239
GlaI GCGC 3 cut(s) 88, 382, 508
GluI GCNGC 1 cut(s) 518
GsaI CCCAGC 2 cut(s) 558, 1056
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 3 cut(s) 243, 270, 1327
HapII CCGG 6 cut(s) 267, 419, 448, 698, 910, 1428
HgaI GACGC 1 cut(s) 857
HhaI GCGC 3 cut(s) 89, 383, 509
Hin1II CATG 5 cut(s) 644, 973, 1074, 1291, 1360
Hin6I GCGC 3 cut(s) 87, 381, 507
HinP1I GCGC 3 cut(s) 87, 381, 507
HinfI GANTC 5 cut(s) 275, 314, 743, 778, 1397
HpaII CCGG 6 cut(s) 267, 419, 448, 698, 910, 1428
Hpy166II GTNNAC 5 cut(s) 208, 592, 735, 1106, 1351
Hpy188I TCNGA 3 cut(s) 772, 1033, 1165
Hpy188III TCNNGA 6 cut(s) 353, 419, 496, 926, 1337, 1409
Hpy8I GTNNAC 5 cut(s) 208, 592, 735, 1106, 1351
Hpy99I CGWCG 4 cut(s) 467, 476, 1266, 1343
HpyCH4III ACNGT 6 cut(s) 375, 761, 1008, 1278, 1343, 1457
HpyCH4IV ACGT 3 cut(s) 474, 678, 1460
HpyCH4V TGCA 6 cut(s) 517, 847, 1045, 1088, 1093, 1346
HpyF10VI GCNNNNNNNGC 3 cut(s) 467, 692, 874
HpyF3I CTNAG 3 cut(s) 609, 919, 1226
HpySE526I ACGT 3 cut(s) 474, 678, 1460
Hsp92II CATG 5 cut(s) 644, 973, 1074, 1291, 1360
HspAI GCGC 3 cut(s) 87, 381, 507
Kpn2I TCCGGA 1 cut(s) 418
Ksp22I TGATCA 1 cut(s) 1389
Kzo9I GATC 2 cut(s) 227, 1389
LmnI GCTCC 2 cut(s) 421, 893
Lsp1109I GCAGC 1 cut(s) 529
LweI GCATC 5 cut(s) 448, 1054, 1075, 1183, 1329
MaeI CTAG 2 cut(s) 212, 1239
MaeII ACGT 3 cut(s) 474, 678, 1460
MaeIII GTNAC 3 cut(s) 761, 795, 1121
MalI GATC 2 cut(s) 229, 1391
MboI GATC 2 cut(s) 227, 1389
MflI RGATCY 1 cut(s) 227
MhlI GDGCHC 3 cut(s) 17, 426, 822
MluCI AATT 4 cut(s) 193, 338, 512, 1329
MlyI GAGTC 1 cut(s) 308
MroI TCCGGA 1 cut(s) 418
MseI TTAA 3 cut(s) 369, 888, 951
MslI CAYNNNNRTG 2 cut(s) 791, 1314
MspA1I CMGCKG 2 cut(s) 520, 1052
MspI CCGG 6 cut(s) 267, 419, 448, 698, 910, 1428
MspR9I CCNGG 7 cut(s) 448, 449, 698, 748, 911, 1314, 1428
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 2 cut(s) 748, 1314
MvnI CGCG 1 cut(s) 497
MwoI GCNNNNNNNGC 3 cut(s) 467, 692, 874
NciI CCSGG 5 cut(s) 448, 449, 698, 911, 1428
NdeII GATC 2 cut(s) 227, 1389
NlaIII CATG 5 cut(s) 644, 973, 1074, 1291, 1360
NlaIV GGNNCC 4 cut(s) 16, 271, 404, 908
NmeAIII GCCGAG 1 cut(s) 409
NmuCI GTSAC 2 cut(s) 795, 1121
NruI TCGCGA 1 cut(s) 497
NspI RCATGY 1 cut(s) 1360
NspV TTCGAA 1 cut(s) 1143
OliI CACNNNNGTG 1 cut(s) 791
PciI ACATGT 1 cut(s) 1356
PcsI WCGNNNNNNNCGW 3 cut(s) 462, 471, 480
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 4 cut(s) 275, 743, 778, 1397
PkrI GCNGC 1 cut(s) 519
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 1461
PpuMI RGGWCCY 2 cut(s) 403, 1230
PscI ACATGT 1 cut(s) 1356
PshAI GACNNNNGTC 1 cut(s) 1305
Psp5II RGGWCCY 2 cut(s) 403, 1230
Psp6I CCWGG 2 cut(s) 746, 1312
PspFI CCCAGC 2 cut(s) 554, 1052
PspGI CCWGG 2 cut(s) 746, 1312
PspN4I GGNNCC 4 cut(s) 16, 271, 404, 908
PspPI GGNCC 5 cut(s) 269, 403, 703, 1230, 1325
PspPPI RGGWCCY 2 cut(s) 403, 1230
PsuI RGATCY 1 cut(s) 227
PvuII CAGCTG 2 cut(s) 520, 1052
RruI TCGCGA 1 cut(s) 497
RsaI GTAC 5 cut(s) 257, 377, 389, 734, 1459
RsaNI GTAC 5 cut(s) 256, 376, 388, 733, 1458
RseI CAYNNNNRTG 2 cut(s) 791, 1314
SaqAI TTAA 3 cut(s) 369, 888, 951
SatI GCNGC 1 cut(s) 518
Sau3AI GATC 2 cut(s) 227, 1389
Sau96I GGNCC 5 cut(s) 269, 403, 703, 1230, 1325
ScaI AGTACT 2 cut(s) 257, 389
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 7 cut(s) 448, 449, 698, 748, 911, 1314, 1428
SduI GDGCHC 3 cut(s) 17, 426, 822
SfaNI GCATC 5 cut(s) 448, 1054, 1075, 1183, 1329
SfuI TTCGAA 1 cut(s) 1143
SinI GGWCC 3 cut(s) 403, 703, 1230
SmaI CCCGGG 1 cut(s) 449
SmiMI CAYNNNNRTG 2 cut(s) 791, 1314
SmlI CTYRAG 1 cut(s) 502
SmoI CTYRAG 1 cut(s) 502
Sse9I AATT 4 cut(s) 193, 338, 512, 1329
SsiI CCGC 3 cut(s) 18, 280, 701
SspMI CTAG 2 cut(s) 212, 1239
StyD4I CCNGG 7 cut(s) 446, 447, 696, 746, 909, 1312, 1426
TaaI ACNGT 6 cut(s) 375, 761, 1008, 1278, 1343, 1457
TaiI ACGT 3 cut(s) 477, 681, 1463
TaqI TCGA 9 cut(s) 249, 456, 682, 741, 925, 1083, 1143, 1188, 1264
TasI AATT 4 cut(s) 193, 338, 512, 1329
TatI WGTACW 3 cut(s) 255, 375, 387
TfiI GAWTC 4 cut(s) 275, 743, 778, 1397
Tru1I TTAA 3 cut(s) 369, 888, 951
Tru9I TTAA 3 cut(s) 369, 888, 951
TscAI CASTG 7 cut(s) 12, 613, 669, 1000, 1126, 1353, 1460
TseFI GTSAC 2 cut(s) 795, 1121
TseI GCWGC 1 cut(s) 517
Tsp45I GTSAC 2 cut(s) 795, 1121
TspDTI ATGAA 4 cut(s) 27, 151, 1059, 1361
TspGWI ACGGA 2 cut(s) 31, 305
TspMI CCCGGG 1 cut(s) 447
TspRI CASTG 7 cut(s) 12, 613, 669, 1000, 1126, 1353, 1460
VpaK11BI GGWCC 3 cut(s) 403, 703, 1230
XagI CCTNNNNNAGG 1 cut(s) 1143
XapI RAATTY 2 cut(s) 193, 338
XceI RCATGY 1 cut(s) 1360
XcmI CCANNNNNNNNNTGG 1 cut(s) 669
XmaI CCCGGG 1 cut(s) 447
XmiI GTMKAC 2 cut(s) 207, 1105
XspI CTAG 2 cut(s) 212, 1239
ZrmI AGTACT 2 cut(s) 257, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.