MD05G1048000.v1.1

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015198
Physical Location & Seq
Forward (+)
8158245 .. 8159311
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1048000.v1.1.491

Sequence Viewer

Length: 510 bp
ATGTTGGTGGTGGACCATGTTGGGCCTATGAATTTTTATATATGCTATGGAAAAATTGCTGTTAGTATATGCTATGGAAGGCATCATTCTTTGAATTGCTTAGCATTTGGATTGAGTGGTGCAGAGATTGTTTTGAACCCTTCCACCACTGTGAGTGAACTCAGTGAACCGATGTGGCCCATTGAGGCCTGCAACGCTGCAATTGCAAATAGCTACTTTGTTGGATCAATCAACCATGTAGGGACTGAGGTTTTCCCAAATCCATTCACTTTCGGTGATGGGAAGCCAGAGCATATAGATCTCGGGCATTTCTACGGATGCAGCCATGTTTCAGCCTCAGATGCCTCATACACTCCATCTTTGTCACGCCATAGGGATGGCTTGTTGATCTCAGACATGGACCTCAACCTCTGCAGGCAGATAAAGACAAACTTGCTTGCTCGTTACTTGAAGCTGGATTTTGAGCCCCAAGTCATTTCTGATCCTTGGTTACATAATAAGTCTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001505 GO:0001889 GO:0003674 GO:0003824 GO:0003837 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006206 GO:0006207 GO:0006208 GO:0006210 GO:0006212 GO:0006213 GO:0006520 GO:0006725 GO:0006807 GO:0007154 GO:0007275 GO:0008150 GO:0008152 GO:0008270 GO:0008652 GO:0009056 GO:0009058 GO:0009112 GO:0009116 GO:0009164 GO:0009605 GO:0009987 GO:0009991 GO:0016053 GO:0016787 GO:0016810 GO:0016811 GO:0017144 GO:0018130 GO:0019439 GO:0019482 GO:0019483 GO:0019752 GO:0019856 GO:0019859 GO:0019860 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033396 GO:0034641 GO:0034655 GO:0034656 GO:0042133 GO:0042136 GO:0042737 GO:0043167 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043292 GO:0043436 GO:0043562 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044444 GO:0044449 GO:0044464 GO:0046112 GO:0046113 GO:0046135 GO:0046394 GO:0046483 GO:0046700 GO:0046872 GO:0046914 GO:0048513 GO:0048731 GO:0048732 GO:0048856 GO:0050896 GO:0051716 GO:0055086 GO:0055120 GO:0061008 GO:0065007 GO:0065008 GO:0071496 GO:0071704 GO:0072527 GO:0072528 GO:0072529 GO:0099080 GO:0099081 GO:0099512 GO:1901135 GO:1901136 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901657 GO:1901658
KEGG Pathways
Metabolic & Signaling
ko00240 ko00410 ko00770 ko00983 ko01100 map00240 map00410 map00770 map00983 map01100
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

170

Amino Acids

18.66

Weight (kDa)

5.77

Isoelectric Point (pI)

32.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 16 - 142 2.6e-15 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015198)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64370
fragaria_vesca FvH4_2g11570
malus_domestica MD05G1048000.v1.1 MD10G1054600.v1.1
prunus_persica Prupe.8G070200_v2.0.a1
pyrus_communis pycom10g04150
rosa_chinensis RchiOBHm_Chr6g0270931
rosa_laevigata RLG00000013759
rosa_multiflora Rmu_sc0010958.1_g000001
rosa_roxburghii Rroxscaffold_7G00197490
rosa_rugosa Rorug06G0057300
rosa_samantha Rh6AG176900 Rh6BG180200 Rh6CG176300 Rh6DG168500
rosa_wichuraiana Rw6G015070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 232, 476
AcsI RAATTY 1 cut(s) 31
AgsI TTSAA 3 cut(s) 94, 136, 451
AleI CACNNNNGTG 1 cut(s) 149
AluBI AGCT 2 cut(s) 213, 454
AluI AGCT 2 cut(s) 213, 454
AlwI GGATC 2 cut(s) 232, 476
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 3 cut(s) 23, 176, 186
ApeKI GCWGC 2 cut(s) 197, 321
ApoI RAATTY 1 cut(s) 31
AspS9I GGNCC 4 cut(s) 13, 23, 177, 400
AsuHPI GGTGA 1 cut(s) 287
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 2 cut(s) 13, 400
BanII GRGCYC 1 cut(s) 468
BbvI GCAGC 2 cut(s) 184, 333
BccI CCATC 3 cut(s) 272, 364, 371
BfmI CTRYAG 1 cut(s) 412
BglII AGATCT 1 cut(s) 298
BisI GCNGC 2 cut(s) 198, 322
BlpI GCTNAGC 1 cut(s) 100
BlsI GCNGC 2 cut(s) 199, 323
Bme18I GGWCC 2 cut(s) 13, 400
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 4 cut(s) 13, 23, 177, 400
BmsI GCATC 3 cut(s) 91, 308, 331
Bpu1102I GCTNAGC 1 cut(s) 100
BsaJI CCNNGG 1 cut(s) 485
BseDI CCNNGG 1 cut(s) 485
BseGI GGATG 2 cut(s) 323, 382
BseMII CTCAG 4 cut(s) 175, 237, 351, 405
BseXI GCAGC 2 cut(s) 184, 333
BsgI GTGCAG 1 cut(s) 141
BshFI GGCC 3 cut(s) 25, 178, 188
BsiHKCI CYCGRG 1 cut(s) 302
BslFI GGGAC 1 cut(s) 256
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 3 cut(s) 25, 178, 188
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 468
Bsp143I GATC 4 cut(s) 224, 298, 387, 481
Bsp1720I GCTNAGC 1 cut(s) 100
BspANI GGCC 3 cut(s) 25, 178, 188
BspCNI CTCAG 4 cut(s) 174, 238, 350, 404
BspMAI CTGCAG 1 cut(s) 416
BspPI GGATC 2 cut(s) 232, 476
BssECI CCNNGG 1 cut(s) 485
BssMI GATC 4 cut(s) 224, 298, 387, 481
BssT1I CCWWGG 1 cut(s) 485
Bst4CI ACNGT 1 cut(s) 151
BstC8I GCNNGC 3 cut(s) 190, 416, 438
BstDEI CTNAG 5 cut(s) 100, 161, 246, 337, 391
BstF5I GGATG 2 cut(s) 323, 382
BstKTI GATC 4 cut(s) 227, 301, 390, 484
BstMBI GATC 4 cut(s) 224, 298, 387, 481
BstMWI GCNNNNNNNGC 3 cut(s) 194, 203, 341
BstSFI CTRYAG 1 cut(s) 412
BstV1I GCAGC 2 cut(s) 184, 333
BstX2I RGATCY 1 cut(s) 298
BstXI CCANNNNNNTGG 1 cut(s) 377
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 3 cut(s) 25, 178, 188
BtsCI GGATG 2 cut(s) 323, 382
BtsIMutI CAGTG 2 cut(s) 147, 169
Cac8I GCNNGC 3 cut(s) 190, 416, 438
Cfr13I GGNCC 4 cut(s) 13, 23, 177, 400
CviAII CATG 4 cut(s) 17, 236, 326, 397
DdeI CTNAG 5 cut(s) 100, 161, 246, 337, 391
DpnI GATC 4 cut(s) 226, 300, 389, 483
DpnII GATC 4 cut(s) 224, 298, 387, 481
Eco130I CCWWGG 1 cut(s) 485
Eco147I AGGCCT 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 468
Eco47I GGWCC 2 cut(s) 13, 400
Eco88I CYCGRG 1 cut(s) 302
EcoT14I CCWWGG 1 cut(s) 485
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 4 cut(s) 20, 239, 329, 400
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 1 cut(s) 256
FatI CATG 4 cut(s) 16, 235, 325, 396
Fnu4HI GCNGC 2 cut(s) 198, 322
FokI GGATG 2 cut(s) 330, 389
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 2 cut(s) 198, 322
GluI GCNGC 2 cut(s) 198, 322
HaeIII GGCC 3 cut(s) 25, 178, 188
Hin1II CATG 4 cut(s) 20, 239, 329, 400
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 3 cut(s) 13, 158, 167
Hpy188I TCNGA 3 cut(s) 340, 394, 481
Hpy8I GTNNAC 3 cut(s) 13, 158, 167
HpyAV CCTTC 2 cut(s) 72, 150
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4V TGCA 6 cut(s) 122, 192, 200, 206, 321, 414
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 203, 341
HpyF3I CTNAG 5 cut(s) 100, 161, 246, 337, 391
Hsp92II CATG 4 cut(s) 20, 239, 329, 400
Kzo9I GATC 4 cut(s) 224, 298, 387, 481
LpnPI CCDG 4 cut(s) 202, 300, 400, 440
Lsp1109I GCAGC 2 cut(s) 184, 333
LweI GCATC 3 cut(s) 91, 308, 331
MaeIII GTNAC 3 cut(s) 363, 443, 489
MalI GATC 4 cut(s) 226, 300, 389, 483
MboI GATC 4 cut(s) 224, 298, 387, 481
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 468
MluCI AATT 4 cut(s) 31, 54, 94, 201
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 6 cut(s) 178, 241, 346, 355, 413, 419
MslI CAYNNNNRTG 2 cut(s) 149, 375
MunI CAATTG 1 cut(s) 201
MwoI GCNNNNNNNGC 3 cut(s) 194, 203, 341
NdeII GATC 4 cut(s) 224, 298, 387, 481
NlaIII CATG 4 cut(s) 20, 239, 329, 400
NmuCI GTSAC 1 cut(s) 363
OliI CACNNNNGTG 1 cut(s) 149
PceI AGGCCT 1 cut(s) 188
PkrI GCNGC 2 cut(s) 199, 323
PspPI GGNCC 4 cut(s) 13, 23, 177, 400
PstI CTGCAG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 298
RseI CAYNNNNRTG 2 cut(s) 149, 375
SatI GCNGC 2 cut(s) 198, 322
Sau3AI GATC 4 cut(s) 224, 298, 387, 481
Sau96I GGNCC 4 cut(s) 13, 23, 177, 400
SduI GDGCHC 1 cut(s) 468
SetI ASST 5 cut(s) 215, 252, 405, 411, 456
SfaNI GCATC 3 cut(s) 91, 308, 331
SfcI CTRYAG 1 cut(s) 412
SinI GGWCC 2 cut(s) 13, 400
SmiMI CAYNNNNRTG 2 cut(s) 149, 375
Sse9I AATT 4 cut(s) 31, 54, 94, 201
SseBI AGGCCT 1 cut(s) 188
StuI AGGCCT 1 cut(s) 188
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 1 cut(s) 151
TasI AATT 4 cut(s) 31, 54, 94, 201
TscAI CASTG 2 cut(s) 154, 169
TseFI GTSAC 1 cut(s) 363
TseI GCWGC 2 cut(s) 197, 321
Tsp45I GTSAC 1 cut(s) 363
TspDTI ATGAA 1 cut(s) 44
TspGWI ACGGA 2 cut(s) 330, 495
TspRI CASTG 2 cut(s) 154, 169
VpaK11BI GGWCC 2 cut(s) 13, 400
XapI RAATTY 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.