Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012712

Physical Location & Seq

Genomic Coordinates Reverse (-)

12710047 .. 12714325

Gene Structure 4279 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1067600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2190 bp

ATGGGTTCCTCTGACGATCAGAATTCGTCATCAGATTCTCCACCGTCGGACTCTTCATCATCTTCGAACAACTCATCGCCTCCCTCCAACAGCAACTCGTCCAAAAATTCAAACAACAACTCAAAAGACTCGGATAATTCATCGTCTTCCAACAACTCGAAAAACTCGCCGCCTTCCAACAACAACAACAATTCCAACGACAACTCCAACAATTCCAACGACAACTCCAACAATTCCAACAACAACTCAAACGGGAACTCAAATGGCAACAAAAATGGCAATTCAAATGGCAACTCGAATGGAAACTCGAACGGCGACTCCTCGAATGGAAACTCGAACGGCGACTCCTCGAATGGAAACTCGAACGGCGACTCAAATGGAAACTCAGACTCAAACGACAACAATAACTCAAACGACAACAACTTGAACAATAACTCCAATTCCCAAAAGTCCCCTCCACAAAACTCCAACTCTCAAAAATCTCCTCCTTCTCAGAACAATGGAGGGGACAAAAACTCCAATTCCCAAAAGTCACCCCCACAAAACTCCAACTCCCAAAAATCTCCCTCTTCCCAGAACAACGGGGACAATAACTCCAACTCCCAAAAGTCCCCTCCACAAAACTCCAATTCCCAAAAATCTCCACCCAAATCATCCCAGGGGAATTGGTCACCGAAGTCGTCTGATGATTGGTCACCCCCAGCACTTGGATCATCAAGCAAGTCACATCGCTCACCATCATCAGACAATGATGGATCGCCGCCACCATCGCACTCAGGGAGCTCCACGCCTATCATAGTAGGAGTTGTAGCTGCGGCAGGGGTGTTCTTGCTTGTCATGGTTTTGTTCTTCTTGGCTTGCTCTAAGAGAAGGAAGAAGAGACAGACTTCTTCCCACCTCATGGAGAACCCTGAACCATATCGTCCAAATGGAAGCGGTGAGTATTGGCAAGGTGAACCTAAAAGCTCCGAGCACGTTCTTAATATGCCTCCTTCTGGCCGCGCGCCAAATGGAGGTTGGACTATGTCATCCGGTGGAGCACCCCCCACGATGAGCAGCGAAATGAGCTCCGCCAACTTCTCCGGTCCACACGGTCCCCCTCTGCCACCTCCACACCCTAATGTGGCATTCGGGTTCAACAAGAGCACCTTCACCTATGAAGAGCTGGCGGCTGCAACCTCAGGGTTTGACCAAGCCAAGCTACTAGGCCAAGGTGGCTTTGGGTTTGTGCACAAAGGGGTGTTGCCAAATGGAAAGGAAATTGCCGTTAAGAGCCTCAAAGCGGGTAGCGGACAAGGTGACCGTGAATTTGCAGCCGAGGTTGAGATCATCAGCCGTGTTCATCATCGCCACCTTGTGTCGCTTGTTGGGTACTGCCTTGCAGGAGAAAAGAAATTGTTGGTGTATGAATATGTTGCTAATAACAACCTCGAGTTCCACCTTCGGGGCGCGAATCGGCCTCCCTTGGAATGGACCAACAGGGTCAAAATTGCTGTGGGATCGGCTAAAGGGCTTGCTTATTTGCACGAAGACTGTCACCCGAAGATTATCCACCGTGACATTAAAGCTGCAAATATTCTGATTGACTATAGTTTCGAGGCTAAGGTGGCAGATTTTGGACTAGCAAAGCTAAACCAGGAAAATGTCACCCATGTATCTACTCGTGTCATGGGAACATTTGGGTATTTGGCTCCTGAGTACGCATCGAGTGGCAAGCTGACCGAGAAGTCTGACGTCTTCTCTTTTGGTATTATGCTTCTGGAGCTGATCACTGGGCGCCCCCCGGTGGACTTGTCTGGGGAATGTGAGGAAGATACCTTAGTAGACTGGGCAAGGCCTCTCTGCATGAAAGCAATAGATACTGGGGACTATTCTGAATTGTTGGATCCACGACTAGAGAACAATTACGACCAGGAACAGGTGGCACGCATGGTGGCGTGTGCGGCTGCGAGTGTTCGACACTCTGCAAAGAAGCGTCCGAAAATGAGCCAGGTTGTTCGTGCATTGGAAGGCGATGTCTCAATGGAAGACTTGCACGAGGGGATTAAAGGGCACTTTGGGTCGTCTGGCTCGGCTTCCACCGATAGTGATAATGATTCGTATAACGCGAAATTGAAAGGGCTTAGGAAGCCGGCGTTGATGGACAGCACAGAATATCCAAGCAGCGAGTACGGTGCTACAACCGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 ko05169 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164 map05169

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

730

Amino Acids

77.37

Weight (kDa)

6.01

Isoelectric Point (pI)

49.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	397 - 599	3.9e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	399 - 669	5.1e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49270 AT3G18810 AT4G34440 AT4G34440
fragaria_vesca	FvH4_2g08640
malus_domestica	MD05G1067600.v1.1 MD10G1077600.v1.1
prunus_persica	Prupe.8G098300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0264041
rosa_laevigata	RLG00000014294
rosa_roxburghii	Rroxscaffold_176G00431380 Rroxscaffold_7G00203640
rosa_rugosa	Rorug06G0007400 Rorug06G0007500
rosa_samantha	Rh6AG128700 Rh6CG123900 Rh6DG110700
rosa_wichuraiana	Rw6G011120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1726
AatII	GACGTC	1 cut(s)	1737
AccB1I	GGYRCC	1 cut(s)	1776
AccB7I	CCANNNNNTGG	2 cut(s)	707, 901
AccI	GTMKAC	1 cut(s)	1824
AccII	CGCG	4 cut(s)	1002, 1004, 1451, 2108
AclWI	GGATC	5 cut(s)	718, 763, 1507, 1880, 1893
AcoI	YGGCCR	1 cut(s)	997
AcsI	RAATTY	3 cut(s)	22, 106, 1307
AcyI	GRCGYC	2 cut(s)	1734, 1777
AdeI	CACNNNGTG	1 cut(s)	1357
AfaI	GTAC	3 cut(s)	1373, 1700, 2171
AgsI	TTSAA	5 cut(s)	111, 285, 427, 1138, 2116
AjnI	CCWGG	4 cut(s)	657, 1635, 1911, 1989
AloI	GAACNNNNNNTCC	6 cut(s)	302, 329, 334, 361, 419, 451
Alw21I	GWGCWC	6 cut(s)	785, 975, 1042, 1070, 1148, 1233
Alw26I	GTCTC	2 cut(s)	874, 2023
Alw44I	GTGCAC	1 cut(s)	1229
AlwI	GGATC	5 cut(s)	718, 763, 1507, 1880, 1893
Ama87I	CYCGRG	1 cut(s)	1430
AoxI	GGCC	4 cut(s)	997, 1207, 1457, 1835
ApaLI	GTGCAC	1 cut(s)	1229
ApeKI	GCWGC	7 cut(s)	812, 1056, 1172, 1313, 1568, 1946, 2163
ApoI	RAATTY	3 cut(s)	22, 106, 1307
ArsI	GACNNNNNNTTYG	4 cut(s)	1292, 1324, 1577, 1609
AspLEI	GCGC	4 cut(s)	1004, 1006, 1451, 1779
AspS9I	GGNCC	3 cut(s)	1085, 1094, 1473
AsuC2I	CCSGG	1 cut(s)	1784
AsuII	TTCGAA	1 cut(s)	65
AvaI	CYCGRG	1 cut(s)	1430
AvaII	GGWCC	3 cut(s)	1085, 1094, 1473
AxyI	CCTNAGG	1 cut(s)	1180
BaeGI	GKGCMC	2 cut(s)	1233, 2055
BaeI	ACNNNNGTAYC	4 cut(s)	1638, 1671, 1806, 1839
BamHI	GGATCC	1 cut(s)	1885
BanI	GGYRCC	1 cut(s)	1776
BanII	GRGCYC	2 cut(s)	785, 1070
BauI	CACGAG	2 cut(s)	1662, 2036
BbsI	GAAGAC	4 cut(s)	138, 1536, 1729, 2034
Bbv12I	GWGCWC	6 cut(s)	785, 975, 1042, 1070, 1148, 1233
BbvI	GCAGC	7 cut(s)	799, 1068, 1159, 1325, 1555, 1933, 2175
BccI	CCATC	4 cut(s)	745, 746, 775, 2134
BceAI	ACGGC	5 cut(s)	328, 355, 382, 1250, 1320
BcgI	CGANNNNNNTGC	2 cut(s)	2156, 2190
BciT130I	CCWGG	4 cut(s)	659, 1637, 1913, 1991
BclI	TGATCA	1 cut(s)	1767
BcnI	CCSGG	1 cut(s)	1784
BcoDI	GTCTC	2 cut(s)	874, 2023
BfaI	CTAG	3 cut(s)	1205, 1622, 1895
BfmI	CTRYAG	1 cut(s)	1588
BfoI	RGCGCY	1 cut(s)	1780
BglI	GCCNNNNNGGC	1 cut(s)	1215
Bme1390I	CCNGG	5 cut(s)	659, 1637, 1784, 1913, 1991
Bme18I	GGWCC	3 cut(s)	1085, 1094, 1473
BmeT110I	CYCGRG	1 cut(s)	1430
BmgT120I	GGNCC	3 cut(s)	1085, 1094, 1473
BmiI	GGNNCC	5 cut(s)	7, 1096, 1692, 1778, 1887
BmrFI	CCNGG	5 cut(s)	659, 1637, 1784, 1913, 1991
BmrI	ACTGGG	3 cut(s)	1782, 1837, 1872
BmsI	GCATC	1 cut(s)	1712
BmuI	ACTGGG	3 cut(s)	1782, 1837, 1872
BpiI	GAAGAC	4 cut(s)	138, 1536, 1729, 2034
BpmI	CTGGAG	1 cut(s)	1781
Bpu10I	CCTNAGC	2 cut(s)	1602, 2123
Bpu14I	TTCGAA	1 cut(s)	65
BpuMI	CCSGG	1 cut(s)	1784
BsaHI	GRCGYC	2 cut(s)	1734, 1777
BsaJI	CCNNGG	6 cut(s)	657, 658, 1210, 1317, 1464, 1782
BsaWI	WCCGGW	2 cut(s)	1031, 1082
Bse118I	RCCGGY	1 cut(s)	2131
Bse1I	ACTGG	3 cut(s)	1777, 1832, 1867
Bse21I	CCTNAGG	1 cut(s)	1180
BseBI	CCWGG	4 cut(s)	659, 1637, 1913, 1991
BseDI	CCNNGG	6 cut(s)	657, 658, 1210, 1317, 1464, 1782
BseGI	GGATG	2 cut(s)	653, 1028
BseMII	CTCAG	5 cut(s)	399, 506, 789, 1194, 1686
BseNI	ACTGG	3 cut(s)	1777, 1832, 1867
BsePI	GCGCGC	1 cut(s)	1002
BseRI	GAGGAG	3 cut(s)	310, 337, 474
BseSI	GKGCMC	2 cut(s)	1233, 2055
BseXI	GCAGC	7 cut(s)	799, 1068, 1159, 1325, 1555, 1933, 2175
BseYI	CCCAGC	1 cut(s)	700
Bsh1236I	CGCG	4 cut(s)	1002, 1004, 1451, 2108
BshFI	GGCC	4 cut(s)	999, 1209, 1459, 1837
BshNI	GGYRCC	1 cut(s)	1776
BsiHKAI	GWGCWC	6 cut(s)	785, 975, 1042, 1070, 1148, 1233
BsiHKCI	CYCGRG	1 cut(s)	1430
BsiSI	CCGG	4 cut(s)	1032, 1083, 1784, 2132
BslFI	GGGAC	6 cut(s)	436, 521, 595, 599, 1080, 1880
BsmAI	GTCTC	2 cut(s)	874, 2023
BsmFI	GGGAC	6 cut(s)	436, 521, 595, 599, 1080, 1880
BsmI	GAATGC	1 cut(s)	1127
BsnI	GGCC	4 cut(s)	999, 1209, 1459, 1837
BsoBI	CYCGRG	1 cut(s)	1430
Bsp119I	TTCGAA	1 cut(s)	65
Bsp1286I	GDGCHC	7 cut(s)	785, 975, 1042, 1070, 1148, 1233, 2055
Bsp143I	GATC	7 cut(s)	16, 710, 755, 1326, 1499, 1767, 1885
BspANI	GGCC	4 cut(s)	999, 1209, 1459, 1837
BspCNI	CTCAG	5 cut(s)	398, 505, 788, 1193, 1687
BspFNI	CGCG	4 cut(s)	1002, 1004, 1451, 2108
BspLI	GGNNCC	5 cut(s)	7, 1096, 1692, 1778, 1887
BspPI	GGATC	5 cut(s)	718, 763, 1507, 1880, 1893
BspQI	GCTCTTC	1 cut(s)	1155
BspT104I	TTCGAA	1 cut(s)	65
BspT107I	GGYRCC	1 cut(s)	1776
BsrFI	RCCGGY	1 cut(s)	2131
BsrI	ACTGG	3 cut(s)	1777, 1832, 1867
BssAI	RCCGGY	1 cut(s)	2131
BssECI	CCNNGG	6 cut(s)	657, 658, 1210, 1317, 1464, 1782
BssHII	GCGCGC	1 cut(s)	1002
BssMI	GATC	7 cut(s)	16, 710, 755, 1326, 1499, 1767, 1885
BssNI	GRCGYC	2 cut(s)	1734, 1777
BssSI	CACGAG	2 cut(s)	1662, 2036
BssT1I	CCWWGG	2 cut(s)	1210, 1464
Bst2BI	CACGAG	2 cut(s)	1662, 2036
Bst2UI	CCWGG	4 cut(s)	659, 1637, 1913, 1991
Bst4CI	ACNGT	6 cut(s)	45, 1094, 1304, 1535, 1556, 2174
Bst6I	CTCTTC	4 cut(s)	58, 574, 872, 1155
BstACI	GRCGYC	2 cut(s)	1734, 1777
BstBI	TTCGAA	1 cut(s)	65
BstC8I	GCNNGC	7 cut(s)	859, 1004, 1167, 1515, 1715, 1927, 2133
BstDEI	CTNAG	9 cut(s)	385, 492, 775, 864, 1180, 1602, 1695, 1819, 2123
BstEII	GGTNACC	3 cut(s)	669, 693, 1298
BstF5I	GGATG	2 cut(s)	653, 1028
BstFNI	CGCG	4 cut(s)	1002, 1004, 1451, 2108
BstH2I	RGCGCY	1 cut(s)	1780
BstHHI	GCGC	4 cut(s)	1004, 1006, 1451, 1779
BstKTI	GATC	7 cut(s)	19, 713, 758, 1329, 1502, 1770, 1888
BstMAI	GTCTC	2 cut(s)	874, 2023
BstMBI	GATC	7 cut(s)	16, 710, 755, 1326, 1499, 1767, 1885
BstMWI	GCNNNNNNNGC	7 cut(s)	769, 1065, 1215, 1607, 1762, 1943, 2128
BstNI	CCWGG	4 cut(s)	659, 1637, 1913, 1991
BstPI	GGTNACC	3 cut(s)	669, 693, 1298
BstSCI	CCNGG	5 cut(s)	657, 1635, 1782, 1911, 1989
BstSFI	CTRYAG	1 cut(s)	1588
BstSLI	GKGCMC	2 cut(s)	1233, 2055
BstUI	CGCG	4 cut(s)	1002, 1004, 1451, 2108
BstV1I	GCAGC	7 cut(s)	799, 1068, 1159, 1325, 1555, 1933, 2175
BstV2I	GAAGAC	4 cut(s)	138, 1536, 1729, 2034
BstX2I	RGATCY	1 cut(s)	1885
BstYI	RGATCY	1 cut(s)	1885
Bsu36I	CCTNAGG	1 cut(s)	1180
BsuRI	GGCC	4 cut(s)	999, 1209, 1459, 1837
BtgZI	GCGATG	5 cut(s)	60, 713, 753, 1331, 2028
BtsCI	GGATG	2 cut(s)	653, 1028
BtsIMutI	CAGTG	1 cut(s)	1770
Cac8I	GCNNGC	7 cut(s)	859, 1004, 1167, 1515, 1715, 1927, 2133
CfoI	GCGC	4 cut(s)	1004, 1006, 1451, 1779
Cfr10I	RCCGGY	1 cut(s)	2131
Cfr13I	GGNCC	3 cut(s)	1085, 1094, 1473
CseI	GACGC	1 cut(s)	1964
Csp6I	GTAC	3 cut(s)	1372, 1699, 2170
CviAII	CATG	6 cut(s)	838, 901, 1652, 1669, 1846, 1930
CviQI	GTAC	3 cut(s)	1372, 1699, 2170
DdeI	CTNAG	9 cut(s)	385, 492, 775, 864, 1180, 1602, 1695, 1819, 2123
DinI	GGCGCC	1 cut(s)	1778
DpnI	GATC	7 cut(s)	18, 712, 757, 1328, 1501, 1769, 1887
DpnII	GATC	7 cut(s)	16, 710, 755, 1326, 1499, 1767, 1885
DraIII	CACNNNGTG	1 cut(s)	1357
DrdI	GACNNNNNNGTC	1 cut(s)	1726
DseDI	GACNNNNNNGTC	1 cut(s)	1726
EaeI	YGGCCR	1 cut(s)	997
Eam1104I	CTCTTC	4 cut(s)	58, 574, 872, 1155
EarI	CTCTTC	4 cut(s)	58, 574, 872, 1155
EciI	GGCGGA	1 cut(s)	1060
Ecl136II	GAGCTC	2 cut(s)	783, 1068
Eco130I	CCWWGG	2 cut(s)	1210, 1464
Eco147I	AGGCCT	1 cut(s)	1837
Eco24I	GRGCYC	2 cut(s)	785, 1070
Eco47I	GGWCC	3 cut(s)	1085, 1094, 1473
Eco53kI	GAGCTC	2 cut(s)	783, 1068
Eco81I	CCTNAGG	1 cut(s)	1180
Eco88I	CYCGRG	1 cut(s)	1430
Eco91I	GGTNACC	3 cut(s)	669, 693, 1298
EcoICRI	GAGCTC	2 cut(s)	783, 1068
EcoO65I	GGTNACC	3 cut(s)	669, 693, 1298
EcoRI	GAATTC	1 cut(s)	22
EcoRII	CCWGG	4 cut(s)	657, 1635, 1911, 1989
EcoT14I	CCWWGG	2 cut(s)	1210, 1464
EcoT38I	GRGCYC	2 cut(s)	785, 1070
EgeI	GGCGCC	1 cut(s)	1778
EheI	GGCGCC	1 cut(s)	1778
ErhI	CCWWGG	2 cut(s)	1210, 1464
FaeI	CATG	6 cut(s)	841, 904, 1655, 1672, 1849, 1933
FalI	AAGNNNNNCTT	2 cut(s)	1133, 1165
FaqI	GGGAC	6 cut(s)	436, 521, 595, 599, 1080, 1880
FatI	CATG	6 cut(s)	837, 900, 1651, 1668, 1845, 1929
FauI	CCCGC	1 cut(s)	1276
FbaI	TGATCA	1 cut(s)	1767
FblI	GTMKAC	1 cut(s)	1824
FokI	GGATG	2 cut(s)	640, 1015
FriOI	GRGCYC	2 cut(s)	785, 1070
FspBI	CTAG	3 cut(s)	1205, 1622, 1895
GlaI	GCGC	4 cut(s)	1003, 1005, 1450, 1778
GsaI	CCCAGC	1 cut(s)	704
GsuI	CTGGAG	1 cut(s)	1781
HaeII	RGCGCY	1 cut(s)	1780
HaeIII	GGCC	4 cut(s)	999, 1209, 1459, 1837
HapII	CCGG	4 cut(s)	1032, 1083, 1784, 2132
HgaI	GACGC	1 cut(s)	1964
HhaI	GCGC	4 cut(s)	1004, 1006, 1451, 1779
Hin1I	GRCGYC	2 cut(s)	1734, 1777
Hin1II	CATG	6 cut(s)	841, 904, 1655, 1672, 1849, 1933
Hin6I	GCGC	4 cut(s)	1002, 1004, 1449, 1777
HinP1I	GCGC	4 cut(s)	1002, 1004, 1449, 1777
HinfI	GANTC	9 cut(s)	35, 50, 128, 317, 344, 371, 389, 1453, 2096
HpaII	CCGG	4 cut(s)	1032, 1083, 1784, 2132
Hpy166II	GTNNAC	5 cut(s)	956, 1088, 1231, 1789, 1825
Hpy188III	TCNNGA	2 cut(s)	1694, 1760
Hpy8I	GTNNAC	5 cut(s)	956, 1088, 1231, 1789, 1825
Hpy99I	CGWCG	1 cut(s)	49
HpyAV	CCTTC	7 cut(s)	183, 498, 864, 1002, 1159, 1451, 2003
HpyCH4III	ACNGT	6 cut(s)	45, 1094, 1304, 1535, 1556, 2174
HpyCH4IV	ACGT	2 cut(s)	975, 1734
HpyF10VI	GCNNNNNNNGC	7 cut(s)	769, 1065, 1215, 1607, 1762, 1943, 2128
HpyF3I	CTNAG	9 cut(s)	385, 492, 775, 864, 1180, 1602, 1695, 1819, 2123
HpySE526I	ACGT	2 cut(s)	975, 1734
Hsp92I	GRCGYC	2 cut(s)	1734, 1777
Hsp92II	CATG	6 cut(s)	841, 904, 1655, 1672, 1849, 1933
HspAI	GCGC	4 cut(s)	1002, 1004, 1449, 1777
KasI	GGCGCC	1 cut(s)	1776
KroI	GCCGGC	1 cut(s)	2131
KroNI	GCCGGC	1 cut(s)	2133
Ksp22I	TGATCA	1 cut(s)	1767
Kzo9I	GATC	7 cut(s)	16, 710, 755, 1326, 1499, 1767, 1885
LguI	GCTCTTC	1 cut(s)	1155
LmnI	GCTCC	7 cut(s)	780, 788, 971, 1037, 1073, 1696, 1762
Lsp1109I	GCAGC	7 cut(s)	799, 1068, 1159, 1325, 1555, 1933, 2175
LweI	GCATC	1 cut(s)	1712
MaeI	CTAG	3 cut(s)	1205, 1622, 1895
MaeII	ACGT	2 cut(s)	975, 1734
MaeIII	GTNAC	8 cut(s)	531, 669, 693, 723, 1298, 1535, 1556, 1645
MalI	GATC	7 cut(s)	18, 712, 757, 1328, 1501, 1769, 1887
MboI	GATC	7 cut(s)	16, 710, 755, 1326, 1499, 1767, 1885
MflI	RGATCY	1 cut(s)	1885
MhlI	GDGCHC	7 cut(s)	785, 975, 1042, 1070, 1148, 1233, 2055
Mly113I	GGCGCC	1 cut(s)	1777
MlyI	GAGTC	6 cut(s)	44, 122, 311, 338, 365, 383
MroNI	GCCGGC	1 cut(s)	2131
MseI	TTAA	4 cut(s)	981, 1269, 1563, 2046
MslI	CAYNNNNRTG	1 cut(s)	1119
MspI	CCGG	4 cut(s)	1032, 1083, 1784, 2132
MspR9I	CCNGG	5 cut(s)	659, 1637, 1784, 1913, 1991
Mva1269I	GAATGC	1 cut(s)	1127
MvaI	CCWGG	4 cut(s)	659, 1637, 1913, 1991
MvnI	CGCG	4 cut(s)	1002, 1004, 1451, 2108
MwoI	GCNNNNNNNGC	7 cut(s)	769, 1065, 1215, 1607, 1762, 1943, 2128
NaeI	GCCGGC	1 cut(s)	2133
NarI	GGCGCC	1 cut(s)	1777
NciI	CCSGG	1 cut(s)	1784
NdeII	GATC	7 cut(s)	16, 710, 755, 1326, 1499, 1767, 1885
NgoMIV	GCCGGC	1 cut(s)	2131
NlaIII	CATG	6 cut(s)	841, 904, 1655, 1672, 1849, 1933
NlaIV	GGNNCC	5 cut(s)	7, 1096, 1692, 1778, 1887
NmeAIII	GCCGAG	2 cut(s)	1342, 2051
NmuCI	GTSAC	8 cut(s)	531, 669, 693, 723, 1298, 1535, 1556, 1645
NspV	TTCGAA	1 cut(s)	65
PaeR7I	CTCGAG	1 cut(s)	1430
PasI	CCCWGGG	1 cut(s)	658
PauI	GCGCGC	1 cut(s)	1002
PceI	AGGCCT	1 cut(s)	1837
PciSI	GCTCTTC	1 cut(s)	1155
PcsI	WCGNNNNNNNCGW	1 cut(s)	2105
PctI	GAATGC	1 cut(s)	1127
PdiI	GCCGGC	1 cut(s)	2133
PfeI	GAWTC	3 cut(s)	35, 1453, 2096
PflFI	GACNNNGTC	1 cut(s)	1024
PflMI	CCANNNNNTGG	2 cut(s)	707, 901
PleI	GAGTC	6 cut(s)	44, 122, 311, 338, 365, 383
PluTI	GGCGCC	1 cut(s)	1780
PpsI	GAGTC	6 cut(s)	44, 122, 311, 338, 365, 383
Psp124BI	GAGCTC	2 cut(s)	785, 1070
Psp6I	CCWGG	4 cut(s)	657, 1635, 1911, 1989
PspEI	GGTNACC	3 cut(s)	669, 693, 1298
PspFI	CCCAGC	1 cut(s)	700
PspGI	CCWGG	4 cut(s)	657, 1635, 1911, 1989
PspN4I	GGNNCC	5 cut(s)	7, 1096, 1692, 1778, 1887
PspPI	GGNCC	3 cut(s)	1085, 1094, 1473
PspXI	VCTCGAGB	1 cut(s)	1430
PsuI	RGATCY	1 cut(s)	1885
PsyI	GACNNNGTC	1 cut(s)	1024
PteI	GCGCGC	1 cut(s)	1002
RsaI	GTAC	3 cut(s)	1373, 1700, 2171
RsaNI	GTAC	3 cut(s)	1372, 1699, 2170
RseI	CAYNNNNRTG	1 cut(s)	1119
SacI	GAGCTC	2 cut(s)	785, 1070
SapI	GCTCTTC	1 cut(s)	1155
SaqAI	TTAA	4 cut(s)	981, 1269, 1563, 2046
Sau3AI	GATC	7 cut(s)	16, 710, 755, 1326, 1499, 1767, 1885
Sau96I	GGNCC	3 cut(s)	1085, 1094, 1473
SchI	GAGTC	6 cut(s)	44, 122, 311, 338, 365, 383
ScrFI	CCNGG	5 cut(s)	659, 1637, 1784, 1913, 1991
SduI	GDGCHC	7 cut(s)	785, 975, 1042, 1070, 1148, 1233, 2055
SfaNI	GCATC	1 cut(s)	1712
SfcI	CTRYAG	1 cut(s)	1588
SfoI	GGCGCC	1 cut(s)	1778
Sfr274I	CTCGAG	1 cut(s)	1430
SfuI	TTCGAA	1 cut(s)	65
SinI	GGWCC	3 cut(s)	1085, 1094, 1473
SlaI	CTCGAG	1 cut(s)	1430
SmiMI	CAYNNNNRTG	1 cut(s)	1119
SmlI	CTYRAG	1 cut(s)	1430
SmoI	CTYRAG	1 cut(s)	1430
SseBI	AGGCCT	1 cut(s)	1837
SspDI	GGCGCC	1 cut(s)	1776
SspI	AATATT	1 cut(s)	1576
SspMI	CTAG	3 cut(s)	1205, 1622, 1895
SstI	GAGCTC	2 cut(s)	785, 1070
StuI	AGGCCT	1 cut(s)	1837
StyD4I	CCNGG	5 cut(s)	657, 1635, 1782, 1911, 1989
StyI	CCWWGG	2 cut(s)	1210, 1464
TaaI	ACNGT	6 cut(s)	45, 1094, 1304, 1535, 1556, 2174
TaiI	ACGT	2 cut(s)	978, 1737
TaqII	GACCGA	1 cut(s)	1736
TauI	GCSGC	6 cut(s)	172, 763, 818, 1002, 1172, 1946
TfiI	GAWTC	3 cut(s)	35, 1453, 2096
Tru1I	TTAA	4 cut(s)	981, 1269, 1563, 2046
Tru9I	TTAA	4 cut(s)	981, 1269, 1563, 2046
TscAI	CASTG	1 cut(s)	1777
TseFI	GTSAC	8 cut(s)	531, 669, 693, 723, 1298, 1535, 1556, 1645
TseI	GCWGC	7 cut(s)	812, 1056, 1172, 1313, 1568, 1946, 2163
Tsp45I	GTSAC	8 cut(s)	531, 669, 693, 723, 1298, 1535, 1556, 1645
TspDTI	ATGAA	6 cut(s)	45, 129, 1173, 1331, 1422, 1862
TspRI	CASTG	1 cut(s)	1777
Tth111I	GACNNNGTC	1 cut(s)	1024
Van91I	CCANNNNNTGG	2 cut(s)	707, 901
VneI	GTGCAC	1 cut(s)	1229
VpaK11BI	GGWCC	3 cut(s)	1085, 1094, 1473
XapI	RAATTY	3 cut(s)	22, 106, 1307
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1014, 1217
XhoI	CTCGAG	1 cut(s)	1430
XmiI	GTMKAC	1 cut(s)	1824
XspI	CTAG	3 cut(s)	1205, 1622, 1895
ZraI	GACGTC	1 cut(s)	1735

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.