Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010375

Physical Location & Seq

Genomic Coordinates Reverse (-)

13026495 .. 13045068

Gene Structure 18574 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1068200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6096 bp

ATGGAGGCGTTGATCGAGCTGTGCGATCTGATTGCGGAGCACCCGGGGCAATTCACCGAGAAGCTTTCGTGGATTTGCGGCCGCTGTCCGCCTCCAGAGTTTCTTCTCGGCGGATCGCCGAGGGTTTCGCGGATTCAGCTCAATGCCGTGCTCGCCATCGCTCGCTTCATCTCCAAATGCCCCGATTCCGCCGATCTCCGCCCGAAATCGGTGGTTCTCGAGTTCCTGCGCTCGGTTCCCGCGTCGTTCAATCGGTCGTTTTGGCCCCAATCGTTTGGAAACGACTCGATCGCTTCTTTCTTCTCCGATTTTTTGGGTTACGTTTCTAAAGCCACCGAATTGTCTCCCGATTTTGCTACAGAGATTGCTGGGTTCACTGGTGAGGTCATAGTTTTGGCAATTAGCAGTGGCGGCGAAGATTTAGGGATTTCTCGGGCTTTTCTCATGGCACTGTCGGAACATTTCCCGCCAATTTTGCCTTCCGATGCTGAGAAGTTGATTACAATGCTTATTGATCATTTTGCGGTTTCGGGACCAGTGGTTCAGTCCCCGGTGACACCGAGGCGAATTGGGGCGAACTCGGAGACCTCATCTGCACAGAGCTCCCCAATGAACGGAAACCATTACCAAGCGAATGAGGGTTCCAGCCCCAGAAATGAGGCGAGTAATGTGTCAGGTTCATCAGGTAGTATATCATCAAGGGGGTCGGTGATGATGAATGGGAGCAGCATTGTGTGGAAGAGTGGTGTTGATCAATTAGGTGTGAATTTCGGTCTCGGTGATGGAGGCGGAGCTGTGATGCTTAGGCAGCAAGTTTCTTCATTTGAGGAAGAATCTGTTGAGAATTTGGAGAAGCAAGATATTGCTTTCAAATTGGTAGCACATATTTTGGATAAAGTCCGCATTGATTCTGCGCTTTTGGAGCAGGTCAGATTTATTGCAAAGAGGCAGCTACAGTCTATGTCAGTGTTTCTGAAGATTAGAAAGCGGGATTGGACTGAACATGGGGCACTTTTAAAAGTGAGAATTAATACGAAACTGTCAGTTTATCAAGCTGCTGCCAAGTTGACTCTTAGTTGCCTGTCTTGCTATGATACGGATATGAAATCAGCCAAGAAGTTGGCACATGAGACTCTGGCATTGTTGATGGATGCTTCTGAAGCCTGTTTGCTCTCCGTGTGGCGGAAAATGAAAGTTTGTGAAGAGCTCTTTGCTTCTTTGCTATCTGGGCTTGCACAAATTGCTGTGAAGCGTGGAGGCCAGGCACTACGTATTTTGCTCATCCGCCTAAAACCTGTTGTGTTAACTGTATGCGCACAGGCTGATACTTGGGCAACCAGCCAGGGAGCAACGTTTGAGAGTGTGATGAAGACAAGTTGTGAGATAATTGAATCTTGTTGGACCAAGGAGCGTGCTCCAGTTGACACATTCATAATGGGATTAGCTACAAGTATTCGTCAACGAAATGACTATGAGGAACAGGTTGATAAAGACAAAGAGGCACTTCCTGTTGTGCAGCTTAATGTTATACGCTTGCTAGCCGATCTGAATGTTGCTGTTAAGAAGTCTGAAGTCGTGGACATGATATTACCTCTATTTATTGAAAGCTTAGAAGAGGGCGATGCTTCGAGTCCTAGTTTATTGCGACTTCGGCTCCTTGATGCTGTATCACGCATGGCAAGTTTAGGTTTTGAGAAGTCCTACCGGGAGACTGTTGTTCTAATGACAAGGAGTTACTTAAGTAAACTTTCAAGTTTAGGATCTGCTGAAAGCAAAACTGTGCCACCAGAAGCCACAACAGAGCGCCTTGAGACTCTTCCTGCAGGATTTCTTCTGATTGCTAGTGGTCTTGCAAATGCTAAATTACGTTCAGACTATCGTCACCGTCTACTGTCTTTATGTTCAGATGTAGGCCTCGCTGCTGAATCCAAAAGTGGAAGGAGCGGAGGAGATTTTTTGGGACCACTACTTCCCGCTGTAGCTGAGATATGTTCTGATTTTGATCCTACAGGAGATGTGGAGCCTTCACTTTTAAAGTTATTTCGCAACCTGTGGTTCTATGTTGCTCTTTTTGGCTTAGCACCCCCCGTACAGAATGCTCAGCAACCTGCAAAGCCACTTTCCACTTCACTCAATAGTGTAGGAAGCATGGGTACTATTCCACTACAAGCTGTGGGTGGCCCATACATGTGGAGTGCACAGTGGTCTTCTGCTGTTCAGCGAATTGCTCAAGGAACACCTCCACTTGTTGTTAGCTCAGTGAAATGGCTTGAAGATGAATTGGAACTCAATGCTCTCTACAATCCCGGTAGTCGTCGAGGGAGTGGGAATGAGAAAGCTGCTCTAGCCCAAAGAGCTGCTCTTTCTACTGCTCTAGGAGGGCGAGTTGATGTTGCAGCAATGAACACTATATCAGGTGTGAAAGCTACCTATCTTCTTGCAGTAGCATTCTTGGAAATCATACGATTCAGCAGCAATGGTGGCATCCTCAATGGTGGCACTAGTTCAGCTGCTTCTAGAAGTGCATTCAGTTGTGTTTTTGAATACCTAAAAACTCCAAATCTTGTGCCCGCTGTCTTCCAATGCTTGATGGCAACTGTGCATAGGGCTTTTGAAACAGCAGTGTCGTGGCTGGAAGATCGAATATCTGAAACAGGCAATGAAGCTGAGGTTAGGGAATCTACTCTTTCTACCCATGCTTGCTTTCTCATCAAAAGCATGTCACAGAGAGAGGAACACATTCGGGAGGTTGCTGTGAACTTGCTGAGTCAGCTTAAAGATAGGTGTCCACAGGTCTTGTGGAATTCATCTTGTGTTGATTCCCTGCTATTTTCAATTAATAATGATTCGCCTTCTACTATTGTCAATGATCCTGCTTGGGCGGTGACAGTTCGTTCTTTGTACCAAAAGATTGTTCGGGAGTGGATTGTTAAATCACTTTCACATGCTCCTTGCTCTAGCCAGGGTCTTCTCCAGGAAAAGCTTTGTAAAGCAAACACATGGCAAAGATCTCAGCATACAACTGATGTTGTATCTCTTTTATCAGAAATACGGATTGGTACAGGTAAAATTGATTGCTGGAAAGGCCTACAAACGGCTAATATTCCTGCAGTAATGGCCGCTGCAGCTGCAGCATCAGGAGCAAACTTGAAACTATTAGAAGCCTTCAATTTGGAGGTGCTCAGTACCGGCATAGTTAGTGCAACAGTTAAGTGCAACCATGCTGGAGAAATTGCTGGCATGAGAAGCTTGTATAACAGCATTGGTGGATTTCAATCTGGTACGACACCAACTGGATTTGGTATTGGTGCGGGCCTTCAAAGGTTGATCTCAGGACCTCGTCAACAGACACAAGCCGAGGACGATTCATTTAATGGGATCTTGCTCACAAAGTTTGTGCGTTTACTACAGCAATTTGTGAATTCTGCAGAAAAAGGTGTGGAAGTGGACAAGTCTCAGTTTCGCCAGATTTGTTCTCAGGCAACTGCATTACTACTGTCAAATCTGGGCTCTAATTCAAAATCAAATGTAGAGGGCTTCTCACAACTGCTCCGTCTTCTCTGTTGGTGTCCAGCTTACATTTCCACGCCTGATGCAATGGAAACTGGCGTTTTTGTCTGGACTTGGTTAGTTTCTGCTGCGCCTGAACTGGGATCTCTAGTCCTTGCGGAGCTTGTTGATGCATGGTTGTGGACCATTGATACAAAACGTGGTATCTTTGCATCTGATGTGAAATATTCTGGCCCTGCAGCAAAATTAAGGCCTCACCTTTCTCCTGGAGAGCCAGAACCGCAGCCTGAAATTGATCCTGTTGAGCAAATAATGGCTCATAGATTATGGCTTGGATTTTTCATGGATCGCTTTGAGGTAGTTCGACACAACAGTGTTGAGCAGCTCTTACTTCTTGGACGAATGTTGCAAGGGATAACAAAGATTCCCTGGAATTTTTCGCGCCATCCTGCTGCTACTGGTACATTTTTTACTGTCATGCTTCTTGGACTGAAGTTCTGCTCATGCCAATCCCAGCGTAATTTGCAGAACTTTAAAACAGGGCTTCAGTTGCTGGAAGACCGGATATATAGGACCTCTTTGGGTTGGTTTGCCTATGAGCCTGAATGGTATGATACCAGCTATATGAATTTTTCTCAGAGTGAAGCTCAATCCATCTCCTTATTTGTCCACTACCTTTCAAATGAGCGAGTAGATGCAGCAGTCTATTCTGATTCAAAAGGGAGTGGACGAGAAAATGGGACCACTTTGGTTGATGCGAACGATCAATATCATCCTGTCTGGGGTCAAATGGAGAACTATGCTGTGGGAAGAGAAAAACGGAAGCAGCTCCTTTTGATGTTATGCCAGCACGAGGCTGACAGGCTTGAAGTTTGGTCGCAACCAACTAATACAAAGGAGAGTGCCTCTTCACGGCAAAAAATTAGTTCGGATAAATGGGTTGAGCATGCTAGGACCGCTTTTGCTGTAGATCCTCGAATTGCTTTATCCTTGGCCTCAAGGTTCCCAACAAACACATTTTTGAAGGCTGAAGTTACCCAGTTGGTTCAGTCGCATATCTTGGATATCCGCTCCATTCCTGAAGCATTGCCGTATTTTGTGACCCCAAAAGCAGTTGATGAAAATTCGGCACTTTTGCAACAATTGCCACATTGGGCTGCTTGCTCAATTACACAAGCTCTAGAGTTCCTTATTCCTGCATATAAGGGTCATCCTCGTGTCATGGCATACGTTCTTAGGGTTTTGGAGTCTTATCCTCCAGACCGAGTAACTTTCTTCATGCCACAGCTAGTGCAGGCTCTGCGATATGATGATGAGAGGTTAGTAGAAGGCTATCTGCTTAGAGCAGCTCAAAGAAGTGATATCTTTGCCCATATTTTAATCTGGCACTTACAGGGTGAGACATCTGTTCCAGAATCAGAAAGAAAAGATGACGTCCCTGTGAAGAATAGTTCGTTTCAAGAGTTGTTGCCTCATGTTAGACAGCATATCATTGATGGTTTCAGTCCCAAGGCACTTGATATTTTTCAAAGAGAATTTGATTTCTTTGACAAGGTTACATCTATTTCCGGTGTACTCTTTCCGCTTCCGAAGGAAGAACGCCGAGCTGGTATCCGGAGAGAGTTGGAGAAAATTGAAGTTGAGGGAGAGGATCTCTATTTGCCAACTGCTCCTACCAAGCTTGTAAGGGGAATTCAGGTAGATAGTGGAATACCCTTGCAATCTGCAGCAAAAGTTCCTATAATGATAACATTTAATGTTGTCGATCGGGTTGGGGACCGCAGTGATGTAAAACCACAAGCTTGTATTTTCAAGGTTGGAGATGATTGCCGCCAGGATGTTCTTGCTCTTCAAGTGATTTCCCTGTTGAGAGACATATTTGAAGCAGTTGGAATTAATCTTTATTTGTTTCCGTATGGTGTTCTCCCAACTGGCCCAGAGAGAGGCATTATTGAGGTTGTACCTAATGCACGGAGCAGAAGTCAGATGGGTGAAACTACGGATGGTGGTTTGTTTGAGATTTTTCAACAGGATTATGGACCTGTTGGGTCTGCCAGCTTTGAAGCTGCCCGCGAAAACTTCATCATTAGTAGTGCTGGTTATGCAGTTGCCAGCCTTTTACTTCAACCAAAGGATCGACACAATGGGAATCTTCTGTTTGACAACGTGGGAAGGCTTGTTCATATTGATTTTGGTTTCATCTTGGAAACTTCACCGGGTGGAAACATGCGCTTTGAAAGTGCACACTTTAAGCTCAGCCATGAGATGACACAATTATTAGACCCTTCTGGGGTTATGAAGAGCGATACATGGAACCAATTTGTGAGCTTGTGCGTTAAAGGGTACCTTGCTGCCCGCCGATACATGGATGGGATCATCAACACAGTGTCGTTAATGCTAGACAGTGGGTTGCCTTGCTTCAGCCGCGGTGACCCTATTGGAAATCTTCGTAAGAGATTTCATCCAGAGATGAGTGAGCGTGAGGCGGCCAATTTTATGATCCATGTGTGCACAGATGCCTACAACAAATGGACGACCGCTGGGTACGACTTGATACAGTATCTGCAGCAGGGCATCGAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005543 GO:0005546 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0008092 GO:0008150 GO:0008152 GO:0008289 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019637 GO:0023052 GO:0030029 GO:0030036 GO:0030054 GO:0030258 GO:0035091 GO:0035556 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048471 GO:0050789 GO:0050794 GO:0050896 GO:0051015 GO:0051716 GO:0052742 GO:0055044 GO:0065007 GO:0070273 GO:0070300 GO:0071704 GO:0071840 GO:0071944 GO:1901564 GO:1901981 GO:1902936

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04011 ko04070 map00562 map01100 map04011 map04070

Pfam Domains

Protein Families

PI3Ka PI3_PI4_kinase

Protein Analysis

2032

Amino Acids

223.89

Weight (kDa)

6.05

Isoelectric Point (pI)

44.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI4K_N	PF19274	290 - 1470	0.0	PI4-kinase N-terminal region
PI3Ka	PF00613	1519 - 1642	6.1e-23	Phosphoinositide 3-kinase family, accessory domain (PIK domain)
PI3_PI4_kinase	PF00454	1771 - 1978	8.1e-35	Phosphatidylinositol 3- and 4-kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49340 AT1G49340 AT1G49340 AT1G49340
fragaria_vesca	FvH4_2g08470 FvH4_2g08470
malus_domestica	MD05G1068200.v1.1 MD10G1078200.v1.1
prunus_persica	Prupe.8G100100_v2.0.a1 Prupe.8G100100_v2.0.a1
pyrus_communis	pycom10g06150
rosa_chinensis	RchiOBHm_Chr6g0263811
rosa_laevigata	RLG00000014311
rosa_multiflora	Rmu_sc0005300.1_g000009
rosa_roxburghii	Rroxscaffold_176G00431230 Rroxscaffold_7G00203880
rosa_rugosa	Rorug06G0006500
rosa_samantha	Rh6AG125900 Rh6CG120700 Rh6DG108400
rosa_wichuraiana	Rw6G010940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	4947
Acc16I	TGCGCA	1 cut(s)	1315
Acc36I	ACCTGC	2 cut(s)	916, 2116
Acc65I	GGTACC	1 cut(s)	5853
AccB1I	GGYRCC	1 cut(s)	5853
AccBSI	CCGCTC	2 cut(s)	1944, 4581
AccI	GTMKAC	1 cut(s)	1888
AccII	CGCG	5 cut(s)	130, 242, 3952, 5583, 5937
AccIII	TCCGGA	1 cut(s)	5124
AclI	AACGTT	1 cut(s)	1352
AcoI	YGGCCR	3 cut(s)	79, 3117, 5997
AcsI	RAATTY	9 cut(s)	766, 844, 2803, 3421, 3943, 4138, 4633, 5045, 5202
AcuI	CTGAAG	8 cut(s)	995, 1179, 1590, 4022, 4040, 4560, 4611, 5914
AcyI	GRCGYC	1 cut(s)	4944
AdeI	CACNNNGTG	1 cut(s)	5729
AflII	CTTAAG	1 cut(s)	1738
AflIII	ACRYGT	1 cut(s)	2187
AhlI	ACTAGT	1 cut(s)	2501
AjnI	CCWGG	7 cut(s)	1260, 1341, 2961, 2973, 3775, 3938, 5343
AjuI	GAANNNNNNNTTGG	8 cut(s)	463, 495, 976, 1008, 2898, 2930, 3039, 3071
AleI	CACNNNNGTG	1 cut(s)	3882
AloI	GAACNNNNNNTCC	4 cut(s)	525, 557, 5673, 5705
Alw21I	GWGCWC	9 cut(s)	42, 153, 605, 1209, 1417, 2200, 3183, 5755, 6023
Alw26I	GTCTC	9 cut(s)	348, 578, 779, 1124, 1703, 1805, 3459, 4904, 5376
Alw44I	GTGCAC	3 cut(s)	2196, 5751, 6019
AlwNI	CAGNNNCTG	3 cut(s)	4063, 4810, 6073
Ama87I	CYCGRG	3 cut(s)	43, 218, 432
Aor13HI	TCCGGA	1 cut(s)	5124
ApaLI	GTGCAC	3 cut(s)	2196, 5751, 6019
ApoI	RAATTY	9 cut(s)	766, 844, 2803, 3421, 3943, 4138, 4633, 5045, 5202
AseI	ATTAAT	3 cut(s)	1029, 2838, 5406
Asp700I	GAANNNNTTC	1 cut(s)	2739
Asp718I	GGTACC	1 cut(s)	5853
AspLEI	GCGC	7 cut(s)	231, 916, 1316, 1806, 3643, 3954, 5742
AsuC2I	CCSGG	6 cut(s)	44, 45, 551, 1706, 2307, 5727
AsuNHI	GCTAGC	1 cut(s)	1537
AvaI	CYCGRG	3 cut(s)	43, 218, 432
BaeGI	GKGCMC	5 cut(s)	1012, 2200, 2571, 5755, 6023
BanI	GGYRCC	1 cut(s)	5853
BanII	GRGCYC	3 cut(s)	605, 1209, 3512
BarI	GAAGNNNNNNTAC	2 cut(s)	2137, 2169
BauI	CACGAG	2 cut(s)	4361, 4725
BbsI	GAAGAC	6 cut(s)	1376, 2199, 2569, 2960, 3548, 4074
Bbv12I	GWGCWC	9 cut(s)	42, 153, 605, 1209, 1417, 2200, 3183, 5755, 6023
BbvCI	CCTCAGC	1 cut(s)	2667
BceAI	ACGGC	4 cut(s)	131, 3111, 4439, 4585
BcgI	CGANNNNNNTGC	2 cut(s)	14, 48
BciT130I	CCWGG	7 cut(s)	1262, 1343, 2963, 2975, 3777, 3940, 5345
BciVI	GTATCC	1 cut(s)	5132
BclI	TGATCA	2 cut(s)	514, 751
BcnI	CCSGG	6 cut(s)	44, 45, 551, 1706, 2307, 5727
BcoDI	GTCTC	9 cut(s)	348, 578, 779, 1124, 1703, 1805, 3459, 4904, 5376
BcuI	ACTAGT	1 cut(s)	2501
BfoI	RGCGCY	1 cut(s)	1807
BfrI	CTTAAG	1 cut(s)	1738
BfuAI	ACCTGC	2 cut(s)	916, 2116
BfuI	GTATCC	1 cut(s)	5132
BglII	AGATCT	1 cut(s)	3008
BlpI	GCTNAGC	3 cut(s)	2077, 2100, 5765
BmeT110I	CYCGRG	3 cut(s)	43, 218, 432
BmrI	ACTGGG	2 cut(s)	3659, 4543
BmtI	GCTAGC	1 cut(s)	1541
BmuI	ACTGGG	2 cut(s)	3659, 4543
BoxI	GACNNNNGTC	1 cut(s)	1878
BpiI	GAAGAC	6 cut(s)	1376, 2199, 2569, 2960, 3548, 4074
BplI	GAGNNNNNCTC	8 cut(s)	3524, 3556, 4141, 4173, 4400, 4432, 5148, 5180
BpmI	CTGGAG	6 cut(s)	78, 1401, 2957, 3246, 3798, 4752
Bpu10I	CCTNAGC	2 cut(s)	803, 2667
Bpu1102I	GCTNAGC	3 cut(s)	2077, 2100, 5765
BpuEI	CTTGAG	3 cut(s)	1829, 2214, 4492
BpuMI	CCSGG	6 cut(s)	44, 45, 551, 1706, 2307, 5727
BsaAI	YACGTR	1 cut(s)	1271
BsaBI	GATNNNNATC	3 cut(s)	2001, 3274, 4278
BsaHI	GRCGYC	1 cut(s)	4944
BsaI	GGTCTC	2 cut(s)	578, 779
BsaWI	WCCGGW	3 cut(s)	4071, 5078, 5124
BsaXI	ACNNNNNCTCC	8 cut(s)	1638, 1668, 2370, 2400, 3534, 3564, 5476, 5506
Bse118I	RCCGGY	1 cut(s)	3188
Bse1I	ACTGG	9 cut(s)	382, 536, 1418, 3298, 3610, 3654, 3973, 4549, 5446
Bse3DI	GCAATG	5 cut(s)	2406, 2482, 2665, 3603, 4595
Bse8I	GATNNNNATC	3 cut(s)	2001, 3274, 4278
BseAI	TCCGGA	1 cut(s)	5124
BseBI	CCWGG	7 cut(s)	1262, 1343, 2963, 2975, 3777, 3940, 5345
BseJI	GATNNNNATC	3 cut(s)	2001, 3274, 4278
BseMI	GCAATG	5 cut(s)	2406, 2482, 2665, 3603, 4595
BseNI	ACTGG	9 cut(s)	382, 536, 1418, 3298, 3610, 3654, 3973, 4549, 5446
BseRI	GAGGAG	1 cut(s)	1962
BseSI	GKGCMC	5 cut(s)	1012, 2200, 2571, 5755, 6023
BseX3I	CGGCCG	1 cut(s)	79
BseYI	CCCAGC	3 cut(s)	368, 4023, 6050
BsgI	GTGCAG	3 cut(s)	579, 1535, 4823
Bsh1236I	CGCG	5 cut(s)	130, 242, 3952, 5583, 5937
Bsh1285I	CGRYCG	5 cut(s)	82, 257, 291, 5278, 6048
BshNI	GGYRCC	1 cut(s)	5853
BsiEI	CGRYCG	5 cut(s)	82, 257, 291, 5278, 6048
BsiHKAI	GWGCWC	9 cut(s)	42, 153, 605, 1209, 1417, 2200, 3183, 5755, 6023
BsiHKCI	CYCGRG	3 cut(s)	43, 218, 432
BsiSI	CCGG	9 cut(s)	44, 551, 1705, 2307, 3189, 4072, 5079, 5125, 5726
BslFI	GGGAC	7 cut(s)	532, 546, 1974, 4264, 4931, 5001, 5300
BsmAI	GTCTC	9 cut(s)	348, 578, 779, 1124, 1703, 1805, 3459, 4904, 5376
BsmFI	GGGAC	7 cut(s)	532, 546, 1974, 4264, 4931, 5001, 5300
BsmI	GAATGC	3 cut(s)	2101, 2447, 2525
Bso31I	GGTCTC	2 cut(s)	578, 779
BsoBI	CYCGRG	3 cut(s)	43, 218, 432
Bsp13I	TCCGGA	1 cut(s)	5124
Bsp1720I	GCTNAGC	3 cut(s)	2077, 2100, 5765
BspEI	TCCGGA	1 cut(s)	5124
BspFNI	CGCG	5 cut(s)	130, 242, 3952, 5583, 5937
BspMAI	CTGCAG	8 cut(s)	1825, 3112, 3127, 3133, 3430, 3751, 5239, 6078
BspMI	ACCTGC	2 cut(s)	916, 2116
BspOI	GCTAGC	1 cut(s)	1541
BspQI	GCTCTTC	3 cut(s)	1197, 5364, 5804
BspT107I	GGYRCC	1 cut(s)	5853
BspTI	CTTAAG	1 cut(s)	1738
BspTNI	GGTCTC	2 cut(s)	578, 779
BsrBI	CCGCTC	2 cut(s)	1944, 4581
BsrDI	GCAATG	5 cut(s)	2406, 2482, 2665, 3603, 4595
BsrFI	RCCGGY	1 cut(s)	3188
BsrI	ACTGG	9 cut(s)	382, 536, 1418, 3298, 3610, 3654, 3973, 4549, 5446
BssAI	RCCGGY	1 cut(s)	3188
BssNI	GRCGYC	1 cut(s)	4944
BssSI	CACGAG	2 cut(s)	4361, 4725
BssT1I	CCWWGG	3 cut(s)	1404, 4500, 5019
Bst2BI	CACGAG	2 cut(s)	4361, 4725
Bst2UI	CCWGG	7 cut(s)	1262, 1343, 2963, 2975, 3777, 3940, 5345
Bst6I	CTCTTC	8 cut(s)	734, 1197, 1608, 1821, 4315, 4423, 5364, 5804
BstACI	GRCGYC	1 cut(s)	4944
BstAFI	CTTAAG	1 cut(s)	1738
BstAPI	GCANNNNNTGC	3 cut(s)	1241, 4654, 4810
BstBAI	YACGTR	1 cut(s)	1271
BstDSI	CCRYGG	1 cut(s)	5935
BstEII	GGTNACC	1 cut(s)	5939
BstFNI	CGCG	5 cut(s)	130, 242, 3952, 5583, 5937
BstH2I	RGCGCY	1 cut(s)	1807
BstHHI	GCGC	7 cut(s)	231, 916, 1316, 1806, 3643, 3954, 5742
BstMAI	GTCTC	9 cut(s)	348, 578, 779, 1124, 1703, 1805, 3459, 4904, 5376
BstMCI	CGRYCG	5 cut(s)	82, 257, 291, 5278, 6048
BstNI	CCWGG	7 cut(s)	1262, 1343, 2963, 2975, 3777, 3940, 5345
BstNSI	RCATGY	5 cut(s)	2191, 2722, 2948, 4460, 5740
BstPAI	GACNNNNGTC	1 cut(s)	1878
BstPI	GGTNACC	1 cut(s)	5939
BstSLI	GKGCMC	5 cut(s)	1012, 2200, 2571, 5755, 6023
BstSNI	TACGTA	1 cut(s)	1271
BstUI	CGCG	5 cut(s)	130, 242, 3952, 5583, 5937
BstV2I	GAAGAC	6 cut(s)	1376, 2199, 2569, 2960, 3548, 4074
BstX2I	RGATCY	6 cut(s)	1760, 3008, 3378, 3653, 4480, 5161
BstXI	CCANNNNNNTGG	3 cut(s)	275, 1120, 2190
BstYI	RGATCY	6 cut(s)	1760, 3008, 3378, 3653, 4480, 5161
BstZI	CGGCCG	1 cut(s)	79
BsuI	GTATCC	1 cut(s)	5132
BtgI	CCRYGG	1 cut(s)	5935
BtgZI	GCGATG	2 cut(s)	142, 1635
BtsI	GCAGTG	3 cut(s)	412, 2628, 5299
BveI	ACCTGC	2 cut(s)	916, 2116
CaiI	CAGNNNCTG	3 cut(s)	4063, 4810, 6073
CciNI	GCGGCCGC	1 cut(s)	79
CfoI	GCGC	7 cut(s)	231, 916, 1316, 1806, 3643, 3954, 5742
Cfr10I	RCCGGY	1 cut(s)	3188
Cfr42I	CCGCGG	1 cut(s)	5938
Cfr9I	CCCGGG	1 cut(s)	43
CseI	GACGC	1 cut(s)	231
CspCI	CAANNNNNGTGG	4 cut(s)	2778, 2813, 4243, 4278
DraI	TTTAAA	3 cut(s)	1017, 2034, 4045
DraIII	CACNNNGTG	1 cut(s)	5729
EaeI	YGGCCR	3 cut(s)	79, 3117, 5997
EagI	CGGCCG	1 cut(s)	79
Eam1104I	CTCTTC	8 cut(s)	734, 1197, 1608, 1821, 4315, 4423, 5364, 5804
EarI	CTCTTC	8 cut(s)	734, 1197, 1608, 1821, 4315, 4423, 5364, 5804
EciI	GGCGGA	7 cut(s)	78, 126, 178, 188, 804, 1198, 1274
Ecl136II	GAGCTC	2 cut(s)	603, 1207
EclXI	CGGCCG	1 cut(s)	79
Eco105I	TACGTA	1 cut(s)	1271
Eco130I	CCWWGG	3 cut(s)	1404, 4500, 5019
Eco147I	AGGCCT	3 cut(s)	1914, 3087, 3763
Eco24I	GRGCYC	3 cut(s)	605, 1209, 3512
Eco31I	GGTCTC	2 cut(s)	578, 779
Eco32I	GATATC	2 cut(s)	4576, 4873
Eco52I	CGGCCG	1 cut(s)	79
Eco53kI	GAGCTC	2 cut(s)	603, 1207
Eco57I	CTGAAG	8 cut(s)	995, 1179, 1590, 4022, 4040, 4560, 4611, 5914
Eco88I	CYCGRG	3 cut(s)	43, 218, 432
Eco91I	GGTNACC	1 cut(s)	5939
EcoICRI	GAGCTC	2 cut(s)	603, 1207
EcoO109I	RGGNCCY	2 cut(s)	3335, 4083
EcoO65I	GGTNACC	1 cut(s)	5939
EcoRI	GAATTC	3 cut(s)	2803, 3421, 5202
EcoRII	CCWGG	7 cut(s)	1260, 1341, 2961, 2973, 3775, 3938, 5343
EcoRV	GATATC	2 cut(s)	4576, 4873
EcoT14I	CCWWGG	3 cut(s)	1404, 4500, 5019
EcoT22I	ATGCAT	1 cut(s)	3685
EcoT38I	GRGCYC	3 cut(s)	605, 1209, 3512
ErhI	CCWWGG	3 cut(s)	1404, 4500, 5019
FalI	AAGNNNNNCTT	4 cut(s)	1488, 1520, 5841, 5873
FaqI	GGGAC	7 cut(s)	532, 546, 1974, 4264, 4931, 5001, 5300
FauI	CCCGC	8 cut(s)	247, 474, 981, 1981, 2578, 3304, 5588, 5873
FbaI	TGATCA	2 cut(s)	514, 751
FblI	GTMKAC	1 cut(s)	1888
FriOI	GRGCYC	3 cut(s)	605, 1209, 3512
FspAI	RTGCGCAY	1 cut(s)	1315
FspI	TGCGCA	1 cut(s)	1315
GlaI	GCGC	7 cut(s)	230, 915, 1315, 1805, 3642, 3953, 5741
GsaI	CCCAGC	3 cut(s)	372, 4027, 6054
GsuI	CTGGAG	6 cut(s)	78, 1401, 2957, 3246, 3798, 4752
HaeII	RGCGCY	1 cut(s)	1807
HapII	CCGG	9 cut(s)	44, 551, 1705, 2307, 3189, 4072, 5079, 5125, 5726
HgaI	GACGC	1 cut(s)	231
HhaI	GCGC	7 cut(s)	231, 916, 1316, 1806, 3643, 3954, 5742
Hin1I	GRCGYC	1 cut(s)	4944
Hin6I	GCGC	7 cut(s)	229, 914, 1314, 1804, 3641, 3952, 5740
HinP1I	GCGC	7 cut(s)	229, 914, 1314, 1804, 3641, 3952, 5740
HincII	GTYRAC	5 cut(s)	1068, 1305, 1423, 1460, 3344
HindII	GTYRAC	5 cut(s)	1068, 1305, 1423, 1460, 3344
HindIII	AAGCTT	6 cut(s)	62, 1606, 2981, 3247, 5189, 5310
HpaI	GTTAAC	1 cut(s)	1305
HpaII	CCGG	9 cut(s)	44, 551, 1705, 2307, 3189, 4072, 5079, 5125, 5726
Hpy99I	CGWCG	2 cut(s)	247, 2319
HpyCH4IV	ACGT	8 cut(s)	321, 1270, 1352, 1867, 3709, 4740, 4944, 5676
HpySE526I	ACGT	8 cut(s)	321, 1270, 1352, 1867, 3709, 4740, 4944, 5676
Hsp92I	GRCGYC	1 cut(s)	4944
HspAI	GCGC	7 cut(s)	229, 914, 1314, 1804, 3641, 3952, 5740
Kpn2I	TCCGGA	1 cut(s)	5124
KpnI	GGTACC	1 cut(s)	5857
Ksp22I	TGATCA	2 cut(s)	514, 751
KspAI	GTTAAC	1 cut(s)	1305
KspI	CCGCGG	1 cut(s)	5938
LguI	GCTCTTC	3 cut(s)	1197, 5364, 5804
MaeII	ACGT	8 cut(s)	321, 1270, 1352, 1867, 3709, 4740, 4944, 5676
MbiI	CCGCTC	2 cut(s)	1944, 4581
MfeI	CAATTG	1 cut(s)	4652
MflI	RGATCY	6 cut(s)	1760, 3008, 3378, 3653, 4480, 5161
MlyI	GAGTC	7 cut(s)	278, 1063, 1126, 1639, 1807, 2776, 4766
MmeI	TCCRAC	5 cut(s)	435, 1379, 5115, 5308, 5380
Mph1103I	ATGCAT	1 cut(s)	3685
MroI	TCCGGA	1 cut(s)	5124
MroXI	GAANNNNTTC	1 cut(s)	2739
MslI	CAYNNNNRTG	4 cut(s)	2188, 3688, 3882, 4725
MspA1I	CMGCKG	8 cut(s)	84, 1976, 2510, 2573, 3122, 3128, 5937, 6050
MspCI	CTTAAG	1 cut(s)	1738
MspI	CCGG	9 cut(s)	44, 551, 1705, 2307, 3189, 4072, 5079, 5125, 5726
MunI	CAATTG	1 cut(s)	4652
Mva1269I	GAATGC	3 cut(s)	2101, 2447, 2525
MvaI	CCWGG	7 cut(s)	1262, 1343, 2963, 2975, 3777, 3940, 5345
MvnI	CGCG	5 cut(s)	130, 242, 3952, 5583, 5937
NciI	CCSGG	6 cut(s)	44, 45, 551, 1706, 2307, 5727
NheI	GCTAGC	1 cut(s)	1537
NmeAIII	GCCGAG	4 cut(s)	87, 144, 3382, 5138
NmuCI	GTSAC	6 cut(s)	553, 1880, 2721, 2884, 4609, 5939
NotI	GCGGCCGC	1 cut(s)	79
NsbI	TGCGCA	1 cut(s)	1315
NsiI	ATGCAT	1 cut(s)	3685
NspI	RCATGY	5 cut(s)	2191, 2722, 2948, 4460, 5740
OliI	CACNNNNGTG	1 cut(s)	3882
PaeI	GCATGC	1 cut(s)	4460
PaeR7I	CTCGAG	1 cut(s)	218
PceI	AGGCCT	3 cut(s)	1914, 3087, 3763
PciI	ACATGT	1 cut(s)	2187
PciSI	GCTCTTC	3 cut(s)	1197, 5364, 5804
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 239
PctI	GAATGC	3 cut(s)	2101, 2447, 2525
PdmI	GAANNNNTTC	1 cut(s)	2739
PflFI	GACNNNGTC	1 cut(s)	3339
PfoI	TCCNGGA	2 cut(s)	2973, 3775
Ple19I	CGATCG	2 cut(s)	291, 5278
PleI	GAGTC	7 cut(s)	278, 1063, 1126, 1638, 1807, 2775, 4765
PpsI	GAGTC	7 cut(s)	278, 1063, 1126, 1638, 1807, 2775, 4765
Ppu21I	YACGTR	1 cut(s)	1271
PpuMI	RGGWCCY	2 cut(s)	3335, 4083
PscI	ACATGT	1 cut(s)	2187
PshAI	GACNNNNGTC	1 cut(s)	1878
PshBI	ATTAAT	3 cut(s)	1029, 2838, 5406
Psp124BI	GAGCTC	2 cut(s)	605, 1209
Psp1406I	AACGTT	1 cut(s)	1352
Psp5II	RGGWCCY	2 cut(s)	3335, 4083
Psp6I	CCWGG	7 cut(s)	1260, 1341, 2961, 2973, 3775, 3938, 5343
PspEI	GGTNACC	1 cut(s)	5939
PspFI	CCCAGC	3 cut(s)	368, 4023, 6050
PspGI	CCWGG	7 cut(s)	1260, 1341, 2961, 2973, 3775, 3938, 5343
PspPPI	RGGWCCY	2 cut(s)	3335, 4083
PstI	CTGCAG	8 cut(s)	1825, 3112, 3127, 3133, 3430, 3751, 5239, 6078
PstNI	CAGNNNCTG	3 cut(s)	4063, 4810, 6073
PsuI	RGATCY	6 cut(s)	1760, 3008, 3378, 3653, 4480, 5161
PsyI	GACNNNGTC	1 cut(s)	3339
PvuI	CGATCG	2 cut(s)	291, 5278
PvuII	CAGCTG	2 cut(s)	2510, 3128
RseI	CAYNNNNRTG	4 cut(s)	2188, 3688, 3882, 4725
SacI	GAGCTC	2 cut(s)	605, 1209
SacII	CCGCGG	1 cut(s)	5938
SapI	GCTCTTC	3 cut(s)	1197, 5364, 5804
SbfI	CCTGCAGG	1 cut(s)	1825
SchI	GAGTC	7 cut(s)	278, 1063, 1126, 1639, 1807, 2776, 4766
SdaI	CCTGCAGG	1 cut(s)	1825
Sfr274I	CTCGAG	1 cut(s)	218
Sfr303I	CCGCGG	1 cut(s)	5938
SgrBI	CCGCGG	1 cut(s)	5938
SlaI	CTCGAG	1 cut(s)	218
SmaI	CCCGGG	1 cut(s)	45
SmiMI	CAYNNNNRTG	4 cut(s)	2188, 3688, 3882, 4725
SmlI	CTYRAG	5 cut(s)	218, 1738, 1808, 2229, 4507
SmoI	CTYRAG	5 cut(s)	218, 1738, 1808, 2229, 4507
SnaBI	TACGTA	1 cut(s)	1271
SpeI	ACTAGT	1 cut(s)	2501
SphI	GCATGC	1 cut(s)	4460
Sse8387I	CCTGCAGG	1 cut(s)	1825
SseBI	AGGCCT	3 cut(s)	1914, 3087, 3763
SspI	AATATT	2 cut(s)	3103, 3737
SstI	GAGCTC	2 cut(s)	605, 1209
StuI	AGGCCT	3 cut(s)	1914, 3087, 3763
StyI	CCWWGG	3 cut(s)	1404, 4500, 5019
TaiI	ACGT	8 cut(s)	324, 1273, 1355, 1870, 3712, 4743, 4947, 5679
TaqII	GACCGA	3 cut(s)	243, 761, 4788
TatI	WGTACW	1 cut(s)	5083
TauI	GCSGC	7 cut(s)	81, 84, 414, 3122, 5343, 5937, 5999
TseFI	GTSAC	6 cut(s)	553, 1880, 2721, 2884, 4609, 5939
Tsp45I	GTSAC	6 cut(s)	553, 1880, 2721, 2884, 4609, 5939
TspGWI	ACGGA	9 cut(s)	630, 1112, 1165, 3067, 3542, 4345, 5412, 5497, 5525
TspMI	CCCGGG	1 cut(s)	43
Tth111I	GACNNNGTC	1 cut(s)	3339
Vha464I	CTTAAG	1 cut(s)	1738
VneI	GTGCAC	3 cut(s)	2196, 5751, 6019
VspI	ATTAAT	3 cut(s)	1029, 2838, 5406
XapI	RAATTY	9 cut(s)	766, 844, 2803, 3421, 3943, 4138, 4633, 5045, 5202
XbaI	TCTAGA	2 cut(s)	2516, 4690
XceI	RCATGY	5 cut(s)	2191, 2722, 2948, 4460, 5740
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2796
XhoI	CTCGAG	1 cut(s)	218
XmaI	CCCGGG	1 cut(s)	43
XmiI	GTMKAC	1 cut(s)	1888
XmnI	GAANNNNTTC	1 cut(s)	2739
ZraI	GACGTC	1 cut(s)	4945
Zsp2I	ATGCAT	1 cut(s)	3685

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.