Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010375

Physical Location & Seq

Genomic Coordinates Reverse (-)

11143629 .. 11174044

Gene Structure 30416 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1078200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6096 bp

ATGGAGGCATTGATGGAGCTCTGCGACCTGATTGCGGACCACCCGGCGCAATTCACCCAGAAGCTTTCGTGGATTTGTGGCCGGTGTCCGCCTCCCGAGATTTTTCTGGGCGGATCACCTAGGGTTTCGCAGATTCAGCTCAATGCTGTCCTCGCCGTCGCGCGCTTCATGTCCAAATGCCCCGATTCTGCTGATCTCCGCCCGAAATCGGTGGTTCTCGAGTTCCTACGCTCGGTTCCCGCGTCGTTCAATCGGTCGTTTTGGCCCCAATCGTTTGGAAACGACTCGATTGCTTCTTTCTTCTTGGATTTTTTGGGGTACGTGTGTAAAGCTACCGAATTGTCTCCCGATTTTGCTACAGAGATTGTTGGGTTCACTGGTGAGGTTGTAGTTTTGGCAATTACCAGTGGCGGCGAAGATTTAGGTATTTGTCGGGCTTTCCTCATGGCCCTATCAGAACATTTCCTTCCAATTTTGCCTTCGGATGCCGAGAAGTTGATTACAATGCTTATTGACCAGTTTGCGGCTTCGGGACCGGTGGTTCAGTCCCCGGTTACGCCAAGACGAATTGGGGCCAACTCAGAGACCTCGTCTGCGCAGAGCTCCCCAATGAATGGAAACCATTACCAAGCGAATGAGAGCTCCAGCCCCAGAAATGAGGCGAGCAATGTGTCGGGTTCATCGGGTAGTATATCGTCAAGGGGGTCGGTGATGATGAATGGGAGCAGCATTGTGTGGAAGAGTGGTGTTGATCAACTGGGTGTGAATTTCGGTCTTGGTGAGGGAGGCGGAGCTGTGATGCTTAGGCAGCAAGTTTCTTCATTTGAGGAAGAGTCTGTTGAGAATTTGGAGAAGCAAGAGATTGCTTTCAAATTGGTAGCTCATATTTTGGATAAAGTCAGTATCGATTCTGCGCTTTTGGATCGGGTCAGATTAATCGCAAAGAGGCAGCTACAGTCTACGTCAGTGTTTCTGAAGATTAGAAAGCGGGATTGGACTGAACATGGGGCACTTTTGAAAGCAAGAATCAATACGAAACTGTCAGTTTATCAAGCTGCTGCCAAGTTGACTCTTAGTTGCCTGGCTTGCTATGATACTGACGTGAAATCAGCAAAGAAGTTGGCACATGAGAGTCTGGCATTGTTGATGGATGCTTCTGAAGCCTGTTTACTCTCCGTGTGGCGGAAAATGAGAGTTTGTGAAGAGCTCTTTGCTTCTTTGCTATCTGGGCTTGCTCAAATTGCTGTCAAGCGTGGGGGCCAGGCATTACGTATTTTGCTCATCCGCCTCAAACCTGTTGTGTTAACTGTATGCGCACAGGCTGATACTTGGGCAACCAGCCAGGCACCAATGTTTGAGAGTGTGATGAAGACGAGTTGTGAGATAATTGAATCTTGTTGGACTAAGGAGCGTGCGCCGGTTGACACATTCATAATGGGATTAGCGACAAGTATTCGTGAACGAAATGACTATGAGGAACAGGTTGATAAAGATAAAGAGGCACTTCCTGTTGTGCAGCTTAATGTTATACGCTTGCTAGCCGATCTGAATGTTGCTGTGAAGAAGTCTGAAGTTGTGGACATGATATTACCTCTATTTATTGAAAGCTTAGAAGAGGGCGATGCTTCGAGTCCTAGTTTATTGCGACTCCGGCTCCTTGATGCTGTATCACGCATGGCAAGTTTAGGTTTTGAGAAGTCCTACCGGGAGACTGTTGTTCTAATGACAAGGAGTTACTTAAGTAAACTTTCAAGTTTAGGATCTGCTGAAAGCAAAACTGTGCCACCAGAAGCCACAACAGAGCGCCTTGAGACTCTTCCTGCAGGATTTCTTCTGATTGCTAGTGATCTTACAAATGCTAAATTACGTTCAGACTATCGTCACCGTCTACTGTCTTTATGCTCAGATGTAGGCCTCGCTGCTGAATCCAAAAGCGGAAGGAGTGGAGGAGATTTTTTGGGACCACTACTTCTTGCTGTAGCTGAGATATGTTCTGATTTTGATCCTACAGTAGATGTGGAGCCTTCACTTTTAAAGTTATTTCGCAACCTGTGGTTCTATGTTGCTCTTTTTGGCTTAGCACCTCCCATACAGAATGCTCAGCAACCTGCAAAGTCACTTTCCACGTCACTCAATAGTGTAGGAAGCATGGGTACTATTCCACTTCAAGCTGTGGGTGGCCCATACATGTGGAGTGCACAGTGGTCTTCAGCTGTCCAGCAAATTGCTCAAGGAACACCTCCACTTGTTGTTAGCTCAGTGAAATGGCTTGAAGATGAATTGGAACTTAATGCTCTTCAAAATCCCGGCAGTCGTCAAGGGAGTGGGAATGAGAAAGTTGCTCTGGCCCAAAGAGTTGCTCTTTCTACTGCTCTAGGAGGGCGAGTTGAGGTTGCAGCAATAAACACTATATCAGGTGTGAAAGCTACCTATCTTCTTGCGGTAGCATTCTTGGAAATCATACGATTCAGCAGCAATGGTGGCATCCTCAATGGTGTCACTAGTTCAGCTTCTTCTAGAAGTGCATTCAGTTGTGTTTTTGAATACCTAAAAACTCCAAATCTTGTGCCCGCTGTCTTCCAATGCTTGACGGCAACTGTGCATAGGGCTTTTGAAACAGCAGTGACGTGGCTGGAAGATCGAATATCCGAAACAGGCAATGAAGCTGAGGTTAGGGAATCAACACTTTCTACCCATGCTTGCTTTCTGATAAAAAGTATGTCACAGAGAGAGGAACATATCCGGGAAGCTTCTGTGAACTTGCTGAGTCAGCTTAAAGACAGGTTTCCACAGGTCTTGTGGAATTCCTCTTGTGTTGATTCCCTGCTATTTTCAATTTATAATGATTCGCCTACTACTGTTGTCAATGATCCTGCTTGGGTGCTGACAGTTCGTTCTTTGTACCAAAAGATTGTTCGAGAGTGGATCGTTAAATCACTTTCACATGCTCCTTGCTCTAGCCAGGGTCTTCTCCAGGAAAAGCTTTGTAAAGCAAACACGTGGCAAAGATCGCAGCATACAACTGATGTGGTATCTCTTTTATCAGAAATACGAATCGGCACAGGTAAAATTGATTGCTGGAATGGAATACAAACAGCTAATATTCCTGCAGTCATGGCCGCTGCAGCTGCAGCATCAGGAGCAAACTTGAAATTATTAGAAGCCTTCAATTTGGAGGTGCTCAGTACCGGCATCGTTAGTGCAACAGTTAAGTGCAACCATGCTGGAGAAATTGCTGGCATGAGAAGCTTGTATAACAGCATTGGTGGATTTCAATCTGGTACAACACCGACTGGATTTGGTATTGGTGCCGGCCTTCAAAGGTTGATCTCAGGAGCTCGTCAACAGACACAAGCTGAGGACGATTCATTTAATGGAATCTTACTCACAAAGTTTGTGCGTTTACTACAGCAATTTGTTAATTCTGCAGAGAAAGGTGTGGAAGTGGAGAAGTCTCAGTTTCGCCAGATTTGTTCTCAAGCAACTGCATTACTGCTGTCAAATCTGGGCTCTAATTCAAAATCAAATGTAGAGGGCTTCTCACAACTTCTTCGTCTTCTCTGTTGGTGTCCAGCTTACATTTCTACGCCCGACGCAATGGAAACTGGCGTTTTTGTTTGGACTTGGTTAGTTTCTGCTGCGCCTGAACTGGGATCTCTAGTCCTTGCGGAGCTTGTTGATGCATGGTTGTGGACCATTGATACAAGACGTGGTATCTTTGCATCTGATGTGAAACATTCTGGGCCTGCAGCAAAATTAAGGCCTCACCTTTCTCCTGGAGAGCCAGAATCGCAGCCAGACATTGATCCTGTTGAGCAAATAATGGCTCATAGATTATGGCTTGGATTTCTCATGGATCGCTTTGAGGTAGTTCGACACAACAGTGTTGAGCAGCTCTTGCTTCTTGGACGAATGTTGCAAGGGATAACAAAGATTCCCTGGAATTTTTCGCGCCATCCTGCTGCTACTGGTACCTTTTTTACTGTCATGCTTCTTGGACTCAAGTTCTGCTCATGCCAATCCCAGCGAAATTTGCAGAACTTTAAAACAGGGCTTCAGTTGCTGGAAGATCGGATATATAGGACCTCTTTAGGTTGGTTTGCATACGAGCCTGAATGGTATGATACTAGCTATATGAATTTTTCTCAGAGTGAAGCTCAATCCATCTCTTTATTTGTCCACTACCTTTCAAATGAGCGAGTAGATGCAGCAGTCTATTCTGATTCAAAAGGGAGAGGACGAGAAAATGGGACCACTTTGGTTGATGCGAACGATCAACATCATCCAGTCTGGGGTCAAATGGAGAACTATGCTGTGGGAAGAGAAAAACGGAAGCAGCTACTTTTGATGCTATGCCAGCATGAGGGTGACAGGCTTGAAGTTTGGTCGCAACCAACTAATACAAAGGAGAGTGCCTCTTCACGGCAAAAAATTAGTTCGGATAAATGGATTGAGCATGCTAGGACTGCTTTTGCTGTAGATCCTCGAATTGCTTTATCCCTGGCGTCACGGTTCCCAACAAACACATTCTTAAAGGCTGAGGTTACCCAGTTGGTTCAGTCACATATCGTTGATATCCGCTCCATTCCGGAAGCATTGCCATATTTTGTTACCCCAAAGGCAGTTGATGAAAATTCGGCACTTTTGCAACAATTGCCACACTGGGCTGCTTGCTCAATTACACAAGCTCTTGAGTTCCTTACTCCTGCATATAAGGGCCATCCTCGTGTCATGGCATATGTTCTTAGGGTTCTGGAGTCTTATCCTCCTGAACGAGTAACTTTCTTCATGCCACAGCTAGTGCAGGCTCTGCGTTATGATGATGAGAGGTTAGTAGAAGGATATCTGCTTAGAGCAGCACAAAGAAGTGATATATTTGCCCATATTTTAATCTGGCACTTACAGGGTGAGACGTTTGTTCCAGAACCGGAAAAAAAAGATGCTGTTCCTGTGAAGAATAGTTCGTTTCAGGAGTTGTTGCCTCTTGTTAGACAGCATATCATTGATGGTTTCAGTCCCAAGGCACTTGATATTTTTCAAAGAGAATTTGATTTCTTTGACAAGGTTACATCTATTTCTGGTGTACTCTTTCCACTTCCAAAGGAAGAACGCCGAGCTGGTATCCGGAGAGAGTTGGAGAAAATTGAAGTTGAGGGAGAGGATCTCTATTTGCCAACTGCTCCTACCAAGCTTGTAAGGGGCATTCAGGTTGATAGTGGAATACCCTTGCAATCTGCAGCAAAAGTTCCTATAATGATAACATTTAATGTAGTCGATCGGGTTGGGGACCGCAGTGATGTAAAACCACAAGCTTGTATTTTCAAGGTTGGAGATGATTGCCGCCAGGATGTTCTTGCTCTTCAAGTGATTTCCCTATTGAGAGACATATTTGAAGCAGTTGGAATTAATCTTTATTTGTTTCCCTATGGTGTACTCCCAACTGGCCCAGAGAGAGGCATTATTGAGGTTGTACCTAATACGCGGAGCAGAAGTCAGATGGGTGAAACTACTGATGGTGGTTTGTATGAGATTTTTCAACAGGATTACGGACCTGTTGGGTCTGCCAGCTTTGAAGCTGCACGTGAAAACTTCATCATTAGCAGTGCTGGTTATGCAGTTGCCAGCCTTTTACTTCAACCGAAGGATCGACACAATGGGAATCTTCTGTTTGACAGCGTGGGAAGGCTTGTTCATATTGATTTTGGTTTCATTTTGGAAACCTCACCAGGTGGAAACATGCGTTTTGAAAGTGCACACTTTAAGCTCAGCCACGAGATGACACAATTATTAGACCCTTCGGGGGTTATGAAAAGCGATACCTGGAACCAATTTGTGAGCTTGTGCGTTAAGGGTTACCTTGCTGCCCGCCGATACATGGATGGGATTATCAACACGGTATCCCTAATGCTAGACAGTGGGCTACCTTGCTTCAGCCGGGGCGACCCTATTGGAAATCTACGTAAGAGATTTCATCCAGAGATGAGTGAGCGTGAGGCGGCCAATTTTATGATCCATGTATGCACGGATGCCTACAACAAATGGACCACCGCTGGGTACGACTTGATACAGTATCTGCAGCAGGGCATCGAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005543 GO:0005546 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0008092 GO:0008150 GO:0008152 GO:0008289 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019637 GO:0023052 GO:0030029 GO:0030036 GO:0030054 GO:0030258 GO:0035091 GO:0035556 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048471 GO:0050789 GO:0050794 GO:0050896 GO:0051015 GO:0051716 GO:0052742 GO:0055044 GO:0065007 GO:0070273 GO:0070300 GO:0071704 GO:0071840 GO:0071944 GO:1901564 GO:1901981 GO:1902936

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04011 ko04070 map00562 map01100 map04011 map04070

Pfam Domains

Protein Families

PI3Ka PI3_PI4_kinase

Protein Analysis

2032

Amino Acids

224.12

Weight (kDa)

5.95

Isoelectric Point (pI)

43.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI4K_N	PF19274	290 - 1470	0.0	PI4-kinase N-terminal region
PI3Ka	PF00613	1511 - 1644	2.6e-23	Phosphoinositide 3-kinase family, accessory domain (PIK domain)
PI3_PI4_kinase	PF00454	1771 - 1978	2e-35	Phosphatidylinositol 3- and 4-kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49340 AT1G49340 AT1G49340 AT1G49340
fragaria_vesca	FvH4_2g08470 FvH4_2g08470
malus_domestica	MD05G1068200.v1.1 MD10G1078200.v1.1
prunus_persica	Prupe.8G100100_v2.0.a1 Prupe.8G100100_v2.0.a1
pyrus_communis	pycom10g06150
rosa_chinensis	RchiOBHm_Chr6g0263811
rosa_laevigata	RLG00000014311
rosa_multiflora	Rmu_sc0005300.1_g000009
rosa_roxburghii	Rroxscaffold_176G00431230 Rroxscaffold_7G00203880
rosa_rugosa	Rorug06G0006500
rosa_samantha	Rh6AG125900 Rh6CG120700 Rh6DG108400
rosa_wichuraiana	Rw6G010940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2841
Acc16I	TGCGCA	2 cut(s)	597, 1315
Acc36I	ACCTGC	1 cut(s)	2116
Acc65I	GGTACC	1 cut(s)	3971
AccB1I	GGYRCC	3 cut(s)	1345, 3308, 3971
AccB7I	CCANNNNNTGG	1 cut(s)	614
AccBSI	CCGCTC	1 cut(s)	4581
AccI	GTMKAC	2 cut(s)	959, 1888
AccII	CGCG	5 cut(s)	161, 163, 242, 3952, 5482
AccIII	TCCGGA	2 cut(s)	4588, 5124
AcoI	YGGCCR	3 cut(s)	79, 3117, 5997
AcsI	RAATTY	8 cut(s)	766, 844, 2803, 3943, 4030, 4138, 4633, 5045
AcuI	CTGAAG	6 cut(s)	995, 1179, 1590, 2193, 4040, 5914
AcvI	CACGTG	2 cut(s)	3000, 5582
AcyI	GRCGYC	1 cut(s)	4505
AdeI	CACNNNGTG	1 cut(s)	5729
AflII	CTTAAG	1 cut(s)	1738
AflIII	ACRYGT	3 cut(s)	321, 2187, 2997
AgeI	ACCGGT	1 cut(s)	535
AhlI	ACTAGT	1 cut(s)	2501
AjiI	CACGTC	4 cut(s)	1102, 2127, 2628, 3710
AjuI	GAANNNNNNNTTGG	6 cut(s)	463, 495, 976, 1008, 2898, 2930
AleI	CACNNNNGTG	1 cut(s)	3882
AloI	GAACNNNNNNTCC	4 cut(s)	525, 557, 5673, 5705
Alw21I	GWGCWC	8 cut(s)	21, 605, 644, 1209, 2200, 3183, 3340, 5755
Alw26I	GTCTC	7 cut(s)	348, 578, 1703, 1805, 3459, 4904, 5376
Alw44I	GTGCAC	2 cut(s)	2196, 5751
AlwNI	CAGNNNCTG	3 cut(s)	4063, 4810, 6073
Ama87I	CYCGRG	2 cut(s)	95, 218
Aor13HI	TCCGGA	2 cut(s)	4588, 5124
ApaLI	GTGCAC	2 cut(s)	2196, 5751
ApoI	RAATTY	8 cut(s)	766, 844, 2803, 3943, 4030, 4138, 4633, 5045
AseI	ATTAAT	2 cut(s)	935, 5406
AsiGI	ACCGGT	1 cut(s)	535
Asp718I	GGTACC	1 cut(s)	3971
AspA2I	CCTAGG	1 cut(s)	119
AsuC2I	CCSGG	6 cut(s)	44, 551, 1706, 2307, 2744, 5936
AsuNHI	GCTAGC	1 cut(s)	1537
AvaI	CYCGRG	2 cut(s)	95, 218
AvaII	GGWCC	9 cut(s)	37, 533, 1961, 3693, 4083, 4251, 5287, 5549, 6042
AvrII	CCTAGG	1 cut(s)	119
BaeGI	GKGCMC	4 cut(s)	1012, 2200, 2571, 5755
BaeI	ACNNNNGTAYC	4 cut(s)	310, 343, 1086, 1119
BanI	GGYRCC	3 cut(s)	1345, 3308, 3971
BanII	GRGCYC	6 cut(s)	21, 605, 644, 1209, 3340, 3512
BarI	GAAGNNNNNNTAC	4 cut(s)	1152, 1184, 2137, 2169
BauI	CACGAG	2 cut(s)	4725, 5771
BbrPI	CACGTG	2 cut(s)	3000, 5582
BbsI	GAAGAC	5 cut(s)	1376, 2199, 2569, 2960, 3548
Bbv12I	GWGCWC	8 cut(s)	21, 605, 644, 1209, 2200, 3183, 3340, 5755
BbvCI	CCTCAGC	3 cut(s)	2667, 3357, 4539
BccI	CCATC	9 cut(s)	7, 1141, 3963, 4172, 4728, 5000, 5491, 5507, 5873
BceAI	ACGGC	3 cut(s)	140, 2607, 4439
BcgI	CGANNNNNNTGC	4 cut(s)	14, 48, 272, 306
BciVI	GTATCC	2 cut(s)	5132, 5908
BclI	TGATCA	1 cut(s)	751
BcnI	CCSGG	6 cut(s)	44, 551, 1706, 2307, 2744, 5936
BcoDI	GTCTC	7 cut(s)	348, 578, 1703, 1805, 3459, 4904, 5376
BcuI	ACTAGT	1 cut(s)	2501
BfoI	RGCGCY	1 cut(s)	1807
BfrI	CTTAAG	1 cut(s)	1738
BfuAI	ACCTGC	1 cut(s)	2116
BfuI	GTATCC	2 cut(s)	5132, 5908
BlnI	CCTAGG	1 cut(s)	119
BlpI	GCTNAGC	3 cut(s)	2077, 2100, 5765
Bme18I	GGWCC	9 cut(s)	37, 533, 1961, 3693, 4083, 4251, 5287, 5549, 6042
BmeT110I	CYCGRG	2 cut(s)	95, 218
BmgBI	CACGTC	4 cut(s)	1102, 2127, 2628, 3710
BmrI	ACTGGG	4 cut(s)	767, 3659, 4543, 4672
BmtI	GCTAGC	1 cut(s)	1541
BmuI	ACTGGG	4 cut(s)	767, 3659, 4543, 4672
BoxI	GACNNNNGTC	1 cut(s)	1878
BpiI	GAAGAC	5 cut(s)	1376, 2199, 2569, 2960, 3548
BplI	GAGNNNNNCTC	8 cut(s)	3524, 3556, 4141, 4173, 4400, 4432, 5148, 5180
BpmI	CTGGAG	5 cut(s)	628, 2957, 3246, 3798, 4775
Bpu10I	CCTNAGC	4 cut(s)	803, 2667, 3357, 4539
Bpu1102I	GCTNAGC	3 cut(s)	2077, 2100, 5765
BpuEI	CTTGAG	5 cut(s)	1829, 2214, 3462, 3986, 4712
BpuMI	CCSGG	6 cut(s)	44, 551, 1706, 2307, 2744, 5936
Bsa29I	ATCGAT	1 cut(s)	906
BsaAI	YACGTR	5 cut(s)	322, 1271, 3000, 5582, 5960
BsaBI	GATNNNNATC	3 cut(s)	2001, 3274, 4278
BsaHI	GRCGYC	1 cut(s)	4505
BsaI	GGTCTC	1 cut(s)	578
BsaJI	CCNNGG	7 cut(s)	119, 549, 2962, 3938, 4500, 5019, 5935
BsaWI	WCCGGW	4 cut(s)	535, 4588, 4927, 5124
BsaXI	ACNNNNNCTCC	6 cut(s)	1638, 1668, 2370, 2400, 5476, 5506
Bse118I	RCCGGY	5 cut(s)	81, 535, 1417, 3188, 3311
Bse3DI	GCAATG	5 cut(s)	673, 2482, 2665, 3603, 4595
Bse8I	GATNNNNATC	3 cut(s)	2001, 3274, 4278
BseAI	TCCGGA	2 cut(s)	4588, 5124
BseCI	ATCGAT	1 cut(s)	906
BseDI	CCNNGG	7 cut(s)	119, 549, 2962, 3938, 4500, 5019, 5935
BseJI	GATNNNNATC	3 cut(s)	2001, 3274, 4278
BseMI	GCAATG	5 cut(s)	673, 2482, 2665, 3603, 4595
BsePI	GCGCGC	1 cut(s)	161
BseRI	GAGGAG	1 cut(s)	1962
BseSI	GKGCMC	4 cut(s)	1012, 2200, 2571, 5755
BseYI	CCCAGC	2 cut(s)	4023, 6050
BsgI	GTGCAG	3 cut(s)	1535, 4823, 5562
Bsh1236I	CGCG	5 cut(s)	161, 163, 242, 3952, 5482
Bsh1285I	CGRYCG	2 cut(s)	257, 5278
BshNI	GGYRCC	3 cut(s)	1345, 3308, 3971
BshTI	ACCGGT	1 cut(s)	535
BshVI	ATCGAT	1 cut(s)	906
BsiEI	CGRYCG	2 cut(s)	257, 5278
BsiHKAI	GWGCWC	8 cut(s)	21, 605, 644, 1209, 2200, 3183, 3340, 5755
BsiHKCI	CYCGRG	2 cut(s)	95, 218
BslFI	GGGAC	6 cut(s)	532, 546, 1974, 4264, 5001, 5300
BsmAI	GTCTC	7 cut(s)	348, 578, 1703, 1805, 3459, 4904, 5376
BsmBI	CGTCTC	1 cut(s)	4904
BsmFI	GGGAC	6 cut(s)	532, 546, 1974, 4264, 5001, 5300
BsmI	GAATGC	4 cut(s)	2101, 2447, 2525, 5202
Bso31I	GGTCTC	1 cut(s)	578
BsoBI	CYCGRG	2 cut(s)	95, 218
Bsp13I	TCCGGA	2 cut(s)	4588, 5124
Bsp1720I	GCTNAGC	3 cut(s)	2077, 2100, 5765
BspDI	ATCGAT	1 cut(s)	906
BspEI	TCCGGA	2 cut(s)	4588, 5124
BspFNI	CGCG	5 cut(s)	161, 163, 242, 3952, 5482
BspMAI	CTGCAG	8 cut(s)	1825, 3112, 3127, 3133, 3430, 3751, 5239, 6078
BspMI	ACCTGC	1 cut(s)	2116
BspOI	GCTAGC	1 cut(s)	1541
BspQI	GCTCTTC	3 cut(s)	1197, 2301, 5364
BspT107I	GGYRCC	3 cut(s)	1345, 3308, 3971
BspTI	CTTAAG	1 cut(s)	1738
BspTNI	GGTCTC	1 cut(s)	578
BsrBI	CCGCTC	1 cut(s)	4581
BsrDI	GCAATG	5 cut(s)	673, 2482, 2665, 3603, 4595
BsrFI	RCCGGY	5 cut(s)	81, 535, 1417, 3188, 3311
BssAI	RCCGGY	5 cut(s)	81, 535, 1417, 3188, 3311
BssECI	CCNNGG	7 cut(s)	119, 549, 2962, 3938, 4500, 5019, 5935
BssHII	GCGCGC	1 cut(s)	161
BssNI	GRCGYC	1 cut(s)	4505
BssSI	CACGAG	2 cut(s)	4725, 5771
BssT1I	CCWWGG	2 cut(s)	119, 5019
Bst2BI	CACGAG	2 cut(s)	4725, 5771
Bst6I	CTCTTC	9 cut(s)	734, 825, 1197, 1608, 1821, 2301, 4315, 4423, 5364
BstACI	GRCGYC	1 cut(s)	4505
BstAFI	CTTAAG	1 cut(s)	1738
BstAPI	GCANNNNNTGC	3 cut(s)	3898, 4654, 4810
BstBAI	YACGTR	5 cut(s)	322, 1271, 3000, 5582, 5960
BstEII	GGTNACC	2 cut(s)	4543, 5852
BstFNI	CGCG	5 cut(s)	161, 163, 242, 3952, 5482
BstH2I	RGCGCY	1 cut(s)	1807
BstMAI	GTCTC	7 cut(s)	348, 578, 1703, 1805, 3459, 4904, 5376
BstMCI	CGRYCG	2 cut(s)	257, 5278
BstNSI	RCATGY	4 cut(s)	2191, 2948, 4460, 5740
BstPAI	GACNNNNGTC	1 cut(s)	1878
BstPI	GGTNACC	2 cut(s)	4543, 5852
BstSLI	GKGCMC	4 cut(s)	1012, 2200, 2571, 5755
BstSNI	TACGTA	2 cut(s)	1271, 5960
BstUI	CGCG	5 cut(s)	161, 163, 242, 3952, 5482
BstV2I	GAAGAC	5 cut(s)	1376, 2199, 2569, 2960, 3548
BstX2I	RGATCY	4 cut(s)	1760, 3653, 4480, 5161
BstXI	CCANNNNNNTGG	2 cut(s)	275, 2190
BstYI	RGATCY	4 cut(s)	1760, 3653, 4480, 5161
Bsu15I	ATCGAT	1 cut(s)	906
BsuI	GTATCC	2 cut(s)	5132, 5908
BsuTUI	ATCGAT	1 cut(s)	906
BtgZI	GCGATG	1 cut(s)	1635
BtrI	CACGTC	4 cut(s)	1102, 2127, 2628, 3710
BtsI	GCAGTG	3 cut(s)	2628, 5299, 5608
BveI	ACCTGC	1 cut(s)	2116
CaiI	CAGNNNCTG	3 cut(s)	4063, 4810, 6073
Cfr10I	RCCGGY	5 cut(s)	81, 535, 1417, 3188, 3311
ClaI	ATCGAT	1 cut(s)	906
CseI	GACGC	3 cut(s)	231, 3602, 4494
CsiI	ACCWGGT	1 cut(s)	5725
CspAI	ACCGGT	1 cut(s)	535
CspCI	CAANNNNNGTGG	6 cut(s)	2778, 2813, 3009, 3044, 4243, 4278
DraI	TTTAAA	2 cut(s)	2034, 4045
DraIII	CACNNNGTG	1 cut(s)	5729
EaeI	YGGCCR	3 cut(s)	79, 3117, 5997
Eam1104I	CTCTTC	9 cut(s)	734, 825, 1197, 1608, 1821, 2301, 4315, 4423, 5364
EarI	CTCTTC	9 cut(s)	734, 825, 1197, 1608, 1821, 2301, 4315, 4423, 5364
EciI	GGCGGA	6 cut(s)	78, 126, 188, 804, 1198, 1274
Ecl136II	GAGCTC	5 cut(s)	19, 603, 642, 1207, 3338
Eco105I	TACGTA	2 cut(s)	1271, 5960
Eco130I	CCWWGG	2 cut(s)	119, 5019
Eco147I	AGGCCT	2 cut(s)	1914, 3763
Eco24I	GRGCYC	6 cut(s)	21, 605, 644, 1209, 3340, 3512
Eco31I	GGTCTC	1 cut(s)	578
Eco32I	GATATC	2 cut(s)	4576, 4844
Eco47I	GGWCC	9 cut(s)	37, 533, 1961, 3693, 4083, 4251, 5287, 5549, 6042
Eco53kI	GAGCTC	5 cut(s)	19, 603, 642, 1207, 3338
Eco57I	CTGAAG	6 cut(s)	995, 1179, 1590, 2193, 4040, 5914
Eco72I	CACGTG	2 cut(s)	3000, 5582
Eco88I	CYCGRG	2 cut(s)	95, 218
Eco91I	GGTNACC	2 cut(s)	4543, 5852
EcoICRI	GAGCTC	5 cut(s)	19, 603, 642, 1207, 3338
EcoO109I	RGGNCCY	1 cut(s)	4083
EcoO65I	GGTNACC	2 cut(s)	4543, 5852
EcoRI	GAATTC	1 cut(s)	2803
EcoRV	GATATC	2 cut(s)	4576, 4844
EcoT14I	CCWWGG	2 cut(s)	119, 5019
EcoT22I	ATGCAT	1 cut(s)	3685
EcoT38I	GRGCYC	6 cut(s)	21, 605, 644, 1209, 3340, 3512
ErhI	CCWWGG	2 cut(s)	119, 5019
Esp3I	CGTCTC	1 cut(s)	4904
FalI	AAGNNNNNCTT	2 cut(s)	1488, 1520
FaqI	GGGAC	6 cut(s)	532, 546, 1974, 4264, 5001, 5300
FauI	CCCGC	4 cut(s)	247, 981, 2578, 5873
FauNDI	CATATG	1 cut(s)	4738
FbaI	TGATCA	1 cut(s)	751
FblI	GTMKAC	2 cut(s)	959, 1888
FriOI	GRGCYC	6 cut(s)	21, 605, 644, 1209, 3340, 3512
FspAI	RTGCGCAY	1 cut(s)	1315
FspI	TGCGCA	2 cut(s)	597, 1315
GsaI	CCCAGC	2 cut(s)	4027, 6054
GsuI	CTGGAG	5 cut(s)	628, 2957, 3246, 3798, 4775
HaeII	RGCGCY	1 cut(s)	1807
HgaI	GACGC	3 cut(s)	231, 3602, 4494
Hin1I	GRCGYC	1 cut(s)	4505
HincII	GTYRAC	4 cut(s)	1068, 1305, 1423, 3344
HindII	GTYRAC	4 cut(s)	1068, 1305, 1423, 3344
HindIII	AAGCTT	7 cut(s)	62, 1606, 2748, 2981, 3247, 5189, 5310
HpaI	GTTAAC	1 cut(s)	1305
Hpy99I	CGWCG	3 cut(s)	161, 247, 3596
Hsp92I	GRCGYC	1 cut(s)	4505
Kpn2I	TCCGGA	2 cut(s)	4588, 5124
KpnI	GGTACC	1 cut(s)	3975
KroI	GCCGGC	1 cut(s)	3311
KroNI	GCCGGC	1 cut(s)	3313
Ksp22I	TGATCA	1 cut(s)	751
KspAI	GTTAAC	1 cut(s)	1305
LguI	GCTCTTC	3 cut(s)	1197, 2301, 5364
MabI	ACCWGGT	1 cut(s)	5725
MbiI	CCGCTC	1 cut(s)	4581
MfeI	CAATTG	1 cut(s)	4652
MflI	RGATCY	4 cut(s)	1760, 3653, 4480, 5161
MmeI	TCCRAC	4 cut(s)	1379, 5115, 5308, 5380
Mph1103I	ATGCAT	1 cut(s)	3685
MroI	TCCGGA	2 cut(s)	4588, 5124
MroNI	GCCGGC	1 cut(s)	3311
MslI	CAYNNNNRTG	5 cut(s)	2188, 3688, 3882, 4365, 4725
MspA1I	CMGCKG	5 cut(s)	2213, 2573, 3122, 3128, 6050
MspCI	CTTAAG	1 cut(s)	1738
MunI	CAATTG	1 cut(s)	4652
Mva1269I	GAATGC	4 cut(s)	2101, 2447, 2525, 5202
MvnI	CGCG	5 cut(s)	161, 163, 242, 3952, 5482
NaeI	GCCGGC	1 cut(s)	3313
NciI	CCSGG	6 cut(s)	44, 551, 1706, 2307, 2744, 5936
NdeI	CATATG	1 cut(s)	4738
NgoMIV	GCCGGC	1 cut(s)	3311
NheI	GCTAGC	1 cut(s)	1537
NmeAIII	GCCGAG	2 cut(s)	514, 5138
NmuCI	GTSAC	9 cut(s)	1880, 2115, 2127, 2497, 2623, 2721, 4367, 4506, 4560
NsbI	TGCGCA	2 cut(s)	597, 1315
NsiI	ATGCAT	1 cut(s)	3685
NspI	RCATGY	4 cut(s)	2191, 2948, 4460, 5740
OliI	CACNNNNGTG	1 cut(s)	3882
PaeI	GCATGC	1 cut(s)	4460
PaeR7I	CTCGAG	1 cut(s)	218
PauI	GCGCGC	1 cut(s)	161
PceI	AGGCCT	2 cut(s)	1914, 3763
PciI	ACATGT	1 cut(s)	2187
PciSI	GCTCTTC	3 cut(s)	1197, 2301, 5364
PcsI	WCGNNNNNNNCGW	1 cut(s)	239
PctI	GAATGC	4 cut(s)	2101, 2447, 2525, 5202
PdiI	GCCGGC	1 cut(s)	3313
PflFI	GACNNNGTC	1 cut(s)	589
PflMI	CCANNNNNTGG	1 cut(s)	614
PfoI	TCCNGGA	3 cut(s)	2742, 2973, 3775
PinAI	ACCGGT	1 cut(s)	535
Ple19I	CGATCG	1 cut(s)	5278
PmaCI	CACGTG	2 cut(s)	3000, 5582
PmlI	CACGTG	2 cut(s)	3000, 5582
Ppu21I	YACGTR	5 cut(s)	322, 1271, 3000, 5582, 5960
PpuMI	RGGWCCY	1 cut(s)	4083
PscI	ACATGT	1 cut(s)	2187
PshAI	GACNNNNGTC	1 cut(s)	1878
PshBI	ATTAAT	2 cut(s)	935, 5406
PsiI	TTATAA	1 cut(s)	2841
Psp124BI	GAGCTC	5 cut(s)	21, 605, 644, 1209, 3340
Psp5II	RGGWCCY	1 cut(s)	4083
PspCI	CACGTG	2 cut(s)	3000, 5582
PspEI	GGTNACC	2 cut(s)	4543, 5852
PspFI	CCCAGC	2 cut(s)	4023, 6050
PspPPI	RGGWCCY	1 cut(s)	4083
PstI	CTGCAG	8 cut(s)	1825, 3112, 3127, 3133, 3430, 3751, 5239, 6078
PstNI	CAGNNNCTG	3 cut(s)	4063, 4810, 6073
PsuI	RGATCY	4 cut(s)	1760, 3653, 4480, 5161
PsyI	GACNNNGTC	1 cut(s)	589
PteI	GCGCGC	1 cut(s)	161
PvuI	CGATCG	1 cut(s)	5278
PvuII	CAGCTG	2 cut(s)	2213, 3128
RseI	CAYNNNNRTG	5 cut(s)	2188, 3688, 3882, 4365, 4725
SacI	GAGCTC	5 cut(s)	21, 605, 644, 1209, 3340
SapI	GCTCTTC	3 cut(s)	1197, 2301, 5364
SbfI	CCTGCAGG	1 cut(s)	1825
SdaI	CCTGCAGG	1 cut(s)	1825
SexAI	ACCWGGT	1 cut(s)	5725
Sfr274I	CTCGAG	1 cut(s)	218
SinI	GGWCC	9 cut(s)	37, 533, 1961, 3693, 4083, 4251, 5287, 5549, 6042
SlaI	CTCGAG	1 cut(s)	218
SmiMI	CAYNNNNRTG	5 cut(s)	2188, 3688, 3882, 4365, 4725
SmlI	CTYRAG	7 cut(s)	218, 1738, 1808, 2229, 3477, 4001, 4691
SmoI	CTYRAG	7 cut(s)	218, 1738, 1808, 2229, 3477, 4001, 4691
SnaBI	TACGTA	2 cut(s)	1271, 5960
SpeI	ACTAGT	1 cut(s)	2501
SphI	GCATGC	1 cut(s)	4460
Sse8387I	CCTGCAGG	1 cut(s)	1825
SseBI	AGGCCT	2 cut(s)	1914, 3763
SspI	AATATT	1 cut(s)	3103
SstI	GAGCTC	5 cut(s)	21, 605, 644, 1209, 3340
StuI	AGGCCT	2 cut(s)	1914, 3763
StyI	CCWWGG	2 cut(s)	119, 5019
TaqII	GACCGA	2 cut(s)	243, 761
TatI	WGTACW	2 cut(s)	5083, 5431
TauI	GCSGC	5 cut(s)	414, 527, 3122, 5343, 5999
TseFI	GTSAC	9 cut(s)	1880, 2115, 2127, 2497, 2623, 2721, 4367, 4506, 4560
Tsp45I	GTSAC	9 cut(s)	1880, 2115, 2127, 2497, 2623, 2721, 4367, 4506, 4560
TspGWI	ACGGA	4 cut(s)	1165, 4345, 5562, 6038
Tth111I	GACNNNGTC	1 cut(s)	589
Van91I	CCANNNNNTGG	1 cut(s)	614
Vha464I	CTTAAG	1 cut(s)	1738
VneI	GTGCAC	2 cut(s)	2196, 5751
VpaK11BI	GGWCC	9 cut(s)	37, 533, 1961, 3693, 4083, 4251, 5287, 5549, 6042
VspI	ATTAAT	2 cut(s)	935, 5406
XapI	RAATTY	8 cut(s)	766, 844, 2803, 3943, 4030, 4138, 4633, 5045
XbaI	TCTAGA	1 cut(s)	2516
XceI	RCATGY	4 cut(s)	2191, 2948, 4460, 5740
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2796
XhoI	CTCGAG	1 cut(s)	218
XmaJI	CCTAGG	1 cut(s)	119
XmiI	GTMKAC	2 cut(s)	959, 1888
Zsp2I	ATGCAT	1 cut(s)	3685

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.