Belongs to the PI3 PI4-kinase family
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G49340 AT1G49340 AT1G49340 AT1G49340 |
| fragaria_vesca | FvH4_2g08470 FvH4_2g08470 |
| malus_domestica | MD05G1068200.v1.1 MD10G1078200.v1.1 |
| prunus_persica | Prupe.8G100100_v2.0.a1 Prupe.8G100100_v2.0.a1 |
| pyrus_communis | pycom10g06150 |
| rosa_chinensis | RchiOBHm_Chr6g0263811 |
| rosa_laevigata | RLG00000014311 |
| rosa_multiflora | Rmu_sc0005300.1_g000009 |
| rosa_roxburghii | Rroxscaffold_176G00431230 Rroxscaffold_7G00203880 |
| rosa_rugosa | Rorug06G0006500 |
| rosa_samantha | Rh6AG125900 Rh6CG120700 Rh6DG108400 |
| rosa_wichuraiana | Rw6G010940 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 387 |
| Acc16I | TGCGCA | 1 cut(s) | 1277 |
| Acc36I | ACCTGC | 1 cut(s) | 922 |
| Acc65I | GGTACC | 1 cut(s) | 5859 |
| AccB1I | GGYRCC | 4 cut(s) | 91, 1510, 2086, 5859 |
| AccBSI | CCGCTC | 4 cut(s) | 255, 800, 4207, 4590 |
| AccI | GTMKAC | 2 cut(s) | 599, 1584 |
| AccII | CGCG | 3 cut(s) | 35, 581, 5587 |
| AcoI | YGGCCR | 1 cut(s) | 801 |
| AcuI | CTGAAG | 8 cut(s) | 1185, 1596, 2688, 3126, 4049, 4569, 4620, 5920 |
| AcvI | CACGTG | 1 cut(s) | 3006 |
| AcyI | GRCGYC | 3 cut(s) | 92, 384, 560 |
| AfaI | GTAC | 9 cut(s) | 1536, 1864, 2161, 3193, 3982, 4761, 5091, 5478, 5861 |
| AflIII | ACRYGT | 3 cut(s) | 1810, 3003, 5037 |
| AgeI | ACCGGT | 1 cut(s) | 553 |
| AjiI | CACGTC | 1 cut(s) | 5038 |
| AjnI | CCWGG | 9 cut(s) | 1028, 1266, 2311, 2457, 2967, 3784, 3947, 5718, 5825 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1200, 1232, 2904, 2936 |
| Alw44I | GTGCAC | 1 cut(s) | 5757 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 1273, 4072, 4819, 6079 |
| Ama87I | CYCGRG | 4 cut(s) | 120, 3337, 4734, 5981 |
| ApaLI | GTGCAC | 1 cut(s) | 5757 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 754, 786 |
| AseI | ATTAAT | 2 cut(s) | 941, 5412 |
| AsiGI | ACCGGT | 1 cut(s) | 553 |
| Asp718I | GGTACC | 1 cut(s) | 5859 |
| AspLEI | GCGC | 6 cut(s) | 94, 1278, 3802, 4038, 4399, 5587 |
| AsuC2I | CCSGG | 4 cut(s) | 44, 130, 2750, 5733 |
| AsuII | TTCGAA | 1 cut(s) | 5577 |
| AsuNHI | GCTAGC | 2 cut(s) | 1543, 1847 |
| AvaI | CYCGRG | 4 cut(s) | 120, 3337, 4734, 5981 |
| AvaII | GGWCC | 8 cut(s) | 1407, 1967, 2186, 3702, 4473, 5293, 5797, 6048 |
| AxyI | CCTNAGG | 1 cut(s) | 980 |
| BaeGI | GKGCMC | 1 cut(s) | 5761 |
| BanI | GGYRCC | 4 cut(s) | 91, 1510, 2086, 5859 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 1158, 1190, 2143, 2175, 3715, 3747 |
| BauI | CACGAG | 1 cut(s) | 5693 |
| BbrPI | CACGTG | 1 cut(s) | 3006 |
| BbsI | GAAGAC | 7 cut(s) | 684, 1382, 2575, 2966, 3557, 4055, 4083 |
| BbvCI | CCTCAGC | 1 cut(s) | 2673 |
| BceAI | ACGGC | 5 cut(s) | 131, 1298, 2564, 3951, 5387 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 887, 921 |
| BciT130I | CCWGG | 9 cut(s) | 1030, 1268, 2313, 2459, 2969, 3786, 3949, 5720, 5827 |
| BclI | TGATCA | 3 cut(s) | 498, 748, 4282 |
| BcnI | CCSGG | 4 cut(s) | 44, 130, 2750, 5733 |
| BfmI | CTRYAG | 9 cut(s) | 959, 1827, 1983, 2013, 2978, 3114, 3435, 4323, 4485 |
| BfoI | RGCGCY | 2 cut(s) | 95, 3803 |
| BfuAI | ACCTGC | 1 cut(s) | 922 |
| BlpI | GCTNAGC | 1 cut(s) | 5771 |
| BmcAI | AGTACT | 3 cut(s) | 1864, 3193, 4761 |
| Bme18I | GGWCC | 8 cut(s) | 1407, 1967, 2186, 3702, 4473, 5293, 5797, 6048 |
| BmeT110I | CYCGRG | 4 cut(s) | 120, 3337, 4734, 5981 |
| BmgBI | CACGTC | 1 cut(s) | 5038 |
| BmrI | ACTGGG | 2 cut(s) | 3668, 4681 |
| BmtI | GCTAGC | 2 cut(s) | 1547, 1851 |
| BmuI | ACTGGG | 2 cut(s) | 3668, 4681 |
| BoxI | GACNNNNGTC | 2 cut(s) | 84, 1884 |
| BpiI | GAAGAC | 7 cut(s) | 684, 1382, 2575, 2966, 3557, 4055, 4083 |
| BplI | GAGNNNNNCTC | 8 cut(s) | 103, 135, 3533, 3565, 4150, 4182, 5154, 5186 |
| BpmI | CTGGAG | 7 cut(s) | 399, 1407, 2480, 3252, 3476, 4698, 4784 |
| Bpu10I | CCTNAGC | 2 cut(s) | 809, 2673 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 5771 |
| Bpu14I | TTCGAA | 1 cut(s) | 5577 |
| BpuEI | CTTGAG | 2 cut(s) | 3995, 4721 |
| BpuMI | CCSGG | 4 cut(s) | 44, 130, 2750, 5733 |
| BsaAI | YACGTR | 1 cut(s) | 3006 |
| BsaBI | GATNNNNATC | 3 cut(s) | 2007, 3280, 4287 |
| BsaHI | GRCGYC | 3 cut(s) | 92, 384, 560 |
| BsaI | GGTCTC | 2 cut(s) | 138, 5940 |
| BsaJI | CCNNGG | 9 cut(s) | 119, 563, 1348, 1410, 2311, 2968, 3366, 3947, 5025 |
| BsaWI | WCCGGW | 3 cut(s) | 553, 2660, 4080 |
| Bse118I | RCCGGY | 2 cut(s) | 368, 553 |
| Bse21I | CCTNAGG | 1 cut(s) | 980 |
| Bse3DI | GCAATG | 7 cut(s) | 544, 1842, 2341, 2412, 2488, 3612, 4604 |
| Bse8I | GATNNNNATC | 3 cut(s) | 2007, 3280, 4287 |
| BseBI | CCWGG | 9 cut(s) | 1030, 1268, 2313, 2459, 2969, 3786, 3949, 5720, 5827 |
| BseDI | CCNNGG | 9 cut(s) | 119, 563, 1348, 1410, 2311, 2968, 3366, 3947, 5025 |
| BseJI | GATNNNNATC | 3 cut(s) | 2007, 3280, 4287 |
| BseMI | GCAATG | 7 cut(s) | 544, 1842, 2341, 2412, 2488, 3612, 4604 |
| BseRI | GAGGAG | 2 cut(s) | 232, 2235 |
| BseSI | GKGCMC | 1 cut(s) | 5761 |
| BseYI | CCCAGC | 3 cut(s) | 917, 3317, 5570 |
| BsgI | GTGCAG | 4 cut(s) | 1541, 3276, 4832, 6010 |
| Bsh1236I | CGCG | 3 cut(s) | 35, 581, 5587 |
| Bsh1285I | CGRYCG | 3 cut(s) | 82, 253, 5284 |
| BshNI | GGYRCC | 4 cut(s) | 91, 1510, 2086, 5859 |
| BshTI | ACCGGT | 1 cut(s) | 553 |
| BsiEI | CGRYCG | 3 cut(s) | 82, 253, 5284 |
| BsiHKCI | CYCGRG | 4 cut(s) | 120, 3337, 4734, 5981 |
| BslFI | GGGAC | 5 cut(s) | 1458, 3946, 4740, 5237, 5306 |
| BsmBI | CGTCTC | 3 cut(s) | 375, 4180, 4913 |
| BsmFI | GGGAC | 5 cut(s) | 1458, 3946, 4740, 5237, 5306 |
| BsmI | GAATGC | 6 cut(s) | 1458, 2302, 2453, 2531, 4969, 5127 |
| Bso31I | GGTCTC | 2 cut(s) | 138, 5940 |
| BsoBI | CYCGRG | 4 cut(s) | 120, 3337, 4734, 5981 |
| Bsp119I | TTCGAA | 1 cut(s) | 5577 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 5771 |
| BspFNI | CGCG | 3 cut(s) | 35, 581, 5587 |
| BspMAI | CTGCAG | 5 cut(s) | 963, 1831, 3118, 3439, 4327 |
| BspMI | ACCTGC | 1 cut(s) | 922 |
| BspOI | GCTAGC | 2 cut(s) | 1547, 1851 |
| BspQI | GCTCTTC | 3 cut(s) | 1203, 2307, 5370 |
| BspT104I | TTCGAA | 1 cut(s) | 5577 |
| BspT107I | GGYRCC | 4 cut(s) | 91, 1510, 2086, 5859 |
| BspTNI | GGTCTC | 2 cut(s) | 138, 5940 |
| BsrBI | CCGCTC | 4 cut(s) | 255, 800, 4207, 4590 |
| BsrDI | GCAATG | 7 cut(s) | 544, 1842, 2341, 2412, 2488, 3612, 4604 |
| BsrFI | RCCGGY | 2 cut(s) | 368, 553 |
| BssAI | RCCGGY | 2 cut(s) | 368, 553 |
| BssECI | CCNNGG | 9 cut(s) | 119, 563, 1348, 1410, 2311, 2968, 3366, 3947, 5025 |
| BssNI | GRCGYC | 3 cut(s) | 92, 384, 560 |
| BssSI | CACGAG | 1 cut(s) | 5693 |
| BssT1I | CCWWGG | 3 cut(s) | 1348, 1410, 5025 |
| Bst2BI | CACGAG | 1 cut(s) | 5693 |
| Bst2UI | CCWGG | 9 cut(s) | 1030, 1268, 2313, 2459, 2969, 3786, 3949, 5720, 5827 |
| BstACI | GRCGYC | 3 cut(s) | 92, 384, 560 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 967, 3907, 4663, 4819 |
| BstBAI | YACGTR | 1 cut(s) | 3006 |
| BstBI | TTCGAA | 1 cut(s) | 5577 |
| BstEII | GGTNACC | 2 cut(s) | 124, 626 |
| BstFNI | CGCG | 3 cut(s) | 35, 581, 5587 |
| BstH2I | RGCGCY | 2 cut(s) | 95, 3803 |
| BstHHI | GCGC | 6 cut(s) | 94, 1278, 3802, 4038, 4399, 5587 |
| BstMCI | CGRYCG | 3 cut(s) | 82, 253, 5284 |
| BstNI | CCWGG | 9 cut(s) | 1030, 1268, 2313, 2459, 2969, 3786, 3949, 5720, 5827 |
| BstNSI | RCATGY | 2 cut(s) | 974, 1173 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 84, 1884 |
| BstPI | GGTNACC | 2 cut(s) | 124, 626 |
| BstSFI | CTRYAG | 9 cut(s) | 959, 1827, 1983, 2013, 2978, 3114, 3435, 4323, 4485 |
| BstSLI | GKGCMC | 1 cut(s) | 5761 |
| BstUI | CGCG | 3 cut(s) | 35, 581, 5587 |
| BstV2I | GAAGAC | 7 cut(s) | 684, 1382, 2575, 2966, 3557, 4055, 4083 |
| BstX2I | RGATCY | 3 cut(s) | 1766, 4489, 5167 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 1126, 2196, 3383, 5885 |
| BstYI | RGATCY | 3 cut(s) | 1766, 4489, 5167 |
| Bsu36I | CCTNAGG | 1 cut(s) | 980 |
| BtgZI | GCGATG | 1 cut(s) | 1370 |
| BtrI | CACGTC | 1 cut(s) | 5038 |
| BtsI | GCAGTG | 6 cut(s) | 691, 3288, 5099, 5231, 5614, 6051 |
| BveI | ACCTGC | 1 cut(s) | 922 |
| CaiI | CAGNNNCTG | 4 cut(s) | 1273, 4072, 4819, 6079 |
| CfoI | GCGC | 6 cut(s) | 94, 1278, 3802, 4038, 4399, 5587 |
| Cfr10I | RCCGGY | 2 cut(s) | 368, 553 |
| CseI | GACGC | 3 cut(s) | 568, 610, 678 |
| Csp6I | GTAC | 9 cut(s) | 1535, 1863, 2160, 3192, 3981, 4760, 5090, 5477, 5860 |
| CspAI | ACCGGT | 1 cut(s) | 553 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1166, 1201, 2784, 2819 |
| CviQI | GTAC | 9 cut(s) | 1535, 1863, 2160, 3192, 3981, 4760, 5090, 5477, 5860 |
| DinI | GGCGCC | 1 cut(s) | 93 |
| DraI | TTTAAA | 3 cut(s) | 2040, 4054, 4236 |
| EaeI | YGGCCR | 1 cut(s) | 801 |
| EciI | GGCGGA | 4 cut(s) | 188, 804, 1204, 1280 |
| Ecl136II | GAGCTC | 4 cut(s) | 19, 609, 1213, 1421 |
| Eco130I | CCWWGG | 3 cut(s) | 1348, 1410, 5025 |
| Eco147I | AGGCCT | 3 cut(s) | 1920, 3170, 3772 |
| Eco31I | GGTCTC | 2 cut(s) | 138, 5940 |
| Eco32I | GATATC | 1 cut(s) | 4853 |
| Eco47I | GGWCC | 8 cut(s) | 1407, 1967, 2186, 3702, 4473, 5293, 5797, 6048 |
| Eco53kI | GAGCTC | 4 cut(s) | 19, 609, 1213, 1421 |
| Eco57I | CTGAAG | 8 cut(s) | 1185, 1596, 2688, 3126, 4049, 4569, 4620, 5920 |
| Eco72I | CACGTG | 1 cut(s) | 3006 |
| Eco81I | CCTNAGG | 1 cut(s) | 980 |
| Eco88I | CYCGRG | 4 cut(s) | 120, 3337, 4734, 5981 |
| Eco91I | GGTNACC | 2 cut(s) | 124, 626 |
| EcoICRI | GAGCTC | 4 cut(s) | 19, 609, 1213, 1421 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1270, 4092 |
| EcoO65I | GGTNACC | 2 cut(s) | 124, 626 |
| EcoRI | GAATTC | 2 cut(s) | 350, 2809 |
| EcoRII | CCWGG | 9 cut(s) | 1028, 1266, 2311, 2457, 2967, 3784, 3947, 5718, 5825 |
| EcoRV | GATATC | 1 cut(s) | 4853 |
| EcoT14I | CCWWGG | 3 cut(s) | 1348, 1410, 5025 |
| EcoT22I | ATGCAT | 2 cut(s) | 972, 3694 |
| EgeI | GGCGCC | 1 cut(s) | 93 |
| EheI | GGCGCC | 1 cut(s) | 93 |
| ErhI | CCWWGG | 3 cut(s) | 1348, 1410, 5025 |
| Esp3I | CGTCTC | 3 cut(s) | 375, 4180, 4913 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 5343, 5375 |
| FaqI | GGGAC | 5 cut(s) | 1458, 3946, 4740, 5237, 5306 |
| FauI | CCCGC | 1 cut(s) | 4200 |
| FauNDI | CATATG | 3 cut(s) | 2950, 4747, 5431 |
| FbaI | TGATCA | 3 cut(s) | 498, 748, 4282 |
| FblI | GTMKAC | 2 cut(s) | 599, 1584 |
| FspI | TGCGCA | 1 cut(s) | 1277 |
| GlaI | GCGC | 6 cut(s) | 93, 1277, 3801, 4037, 4398, 5586 |
| GsaI | CCCAGC | 3 cut(s) | 921, 3321, 5574 |
| GsuI | CTGGAG | 7 cut(s) | 399, 1407, 2480, 3252, 3476, 4698, 4784 |
| HaeII | RGCGCY | 2 cut(s) | 95, 3803 |
| HgaI | GACGC | 3 cut(s) | 568, 610, 678 |
| HhaI | GCGC | 6 cut(s) | 94, 1278, 3802, 4038, 4399, 5587 |
| Hin1I | GRCGYC | 3 cut(s) | 92, 384, 560 |
| Hin6I | GCGC | 6 cut(s) | 92, 1276, 3800, 4036, 4397, 5585 |
| HinP1I | GCGC | 6 cut(s) | 92, 1276, 3800, 4036, 4397, 5585 |
| HincII | GTYRAC | 4 cut(s) | 600, 1074, 2688, 5011 |
| HindII | GTYRAC | 4 cut(s) | 600, 1074, 2688, 5011 |
| HindIII | AAGCTT | 8 cut(s) | 62, 1612, 1751, 2987, 3941, 5195, 5316, 5768 |
| Hsp92I | GRCGYC | 3 cut(s) | 92, 384, 560 |
| HspAI | GCGC | 6 cut(s) | 92, 1276, 3800, 4036, 4397, 5585 |
| KasI | GGCGCC | 1 cut(s) | 91 |
| KpnI | GGTACC | 1 cut(s) | 5863 |
| KroI | GCCGGC | 1 cut(s) | 368 |
| KroNI | GCCGGC | 1 cut(s) | 370 |
| Ksp22I | TGATCA | 3 cut(s) | 498, 748, 4282 |
| LguI | GCTCTTC | 3 cut(s) | 1203, 2307, 5370 |
| MbiI | CCGCTC | 4 cut(s) | 255, 800, 4207, 4590 |
| MfeI | CAATTG | 1 cut(s) | 5789 |
| MflI | RGATCY | 3 cut(s) | 1766, 4489, 5167 |
| Mly113I | GGCGCC | 1 cut(s) | 92 |
| MmeI | TCCRAC | 6 cut(s) | 1385, 2268, 5121, 5314, 5386, 5566 |
| Mph1103I | ATGCAT | 2 cut(s) | 972, 3694 |
| MroNI | GCCGGC | 1 cut(s) | 368 |
| MslI | CAYNNNNRTG | 5 cut(s) | 2194, 3697, 4254, 5301, 5883 |
| MspA1I | CMGCKG | 5 cut(s) | 84, 958, 1928, 4220, 4561 |
| MunI | CAATTG | 1 cut(s) | 5789 |
| Mva1269I | GAATGC | 6 cut(s) | 1458, 2302, 2453, 2531, 4969, 5127 |
| MvaI | CCWGG | 9 cut(s) | 1030, 1268, 2313, 2459, 2969, 3786, 3949, 5720, 5827 |
| MvnI | CGCG | 3 cut(s) | 35, 581, 5587 |
| NaeI | GCCGGC | 1 cut(s) | 370 |
| NarI | GGCGCC | 1 cut(s) | 92 |
| NciI | CCSGG | 4 cut(s) | 44, 130, 2750, 5733 |
| NdeI | CATATG | 3 cut(s) | 2950, 4747, 5431 |
| NgoMIV | GCCGGC | 1 cut(s) | 368 |
| NheI | GCTAGC | 2 cut(s) | 1543, 1847 |
| NmeAIII | GCCGAG | 4 cut(s) | 385, 514, 588, 3391 |
| NmuCI | GTSAC | 8 cut(s) | 99, 124, 549, 556, 760, 2501, 2890, 4569 |
| NsbI | TGCGCA | 1 cut(s) | 1277 |
| NsiI | ATGCAT | 2 cut(s) | 972, 3694 |
| NspI | RCATGY | 2 cut(s) | 974, 1173 |
| NspV | TTCGAA | 1 cut(s) | 5577 |
| PaeR7I | CTCGAG | 1 cut(s) | 4734 |
| PceI | AGGCCT | 3 cut(s) | 1920, 3170, 3772 |
| PciSI | GCTCTTC | 3 cut(s) | 1203, 2307, 5370 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 593 |
| PctI | GAATGC | 6 cut(s) | 1458, 2302, 2453, 2531, 4969, 5127 |
| PdiI | GCCGGC | 1 cut(s) | 370 |
| PfeI | GAWTC | 6 cut(s) | 1931, 2825, 2852, 3511, 4934, 5209 |
| PflFI | GACNNNGTC | 3 cut(s) | 130, 386, 595 |
| PfoI | TCCNGGA | 3 cut(s) | 2457, 2748, 5718 |
| PinAI | ACCGGT | 1 cut(s) | 553 |
| Ple19I | CGATCG | 1 cut(s) | 5284 |
| PluTI | GGCGCC | 1 cut(s) | 95 |
| PmaCI | CACGTG | 1 cut(s) | 3006 |
| PmlI | CACGTG | 1 cut(s) | 3006 |
| Ppu21I | YACGTR | 1 cut(s) | 3006 |
| PshAI | GACNNNNGTC | 2 cut(s) | 84, 1884 |
| PshBI | ATTAAT | 2 cut(s) | 941, 5412 |
| Psp124BI | GAGCTC | 4 cut(s) | 21, 611, 1215, 1423 |
| Psp6I | CCWGG | 9 cut(s) | 1028, 1266, 2311, 2457, 2967, 3784, 3947, 5718, 5825 |
| PspCI | CACGTG | 1 cut(s) | 3006 |
| PspEI | GGTNACC | 2 cut(s) | 124, 626 |
| PspFI | CCCAGC | 3 cut(s) | 917, 3317, 5570 |
| PspGI | CCWGG | 9 cut(s) | 1028, 1266, 2311, 2457, 2967, 3784, 3947, 5718, 5825 |
| PspXI | VCTCGAGB | 1 cut(s) | 4734 |
| PstI | CTGCAG | 5 cut(s) | 963, 1831, 3118, 3439, 4327 |
| PstNI | CAGNNNCTG | 4 cut(s) | 1273, 4072, 4819, 6079 |
| PsuI | RGATCY | 3 cut(s) | 1766, 4489, 5167 |
| PsyI | GACNNNGTC | 3 cut(s) | 130, 386, 595 |
| PvuI | CGATCG | 1 cut(s) | 5284 |
| PvuII | CAGCTG | 4 cut(s) | 958, 1928, 4220, 4561 |
| RsaI | GTAC | 9 cut(s) | 1536, 1864, 2161, 3193, 3982, 4761, 5091, 5478, 5861 |
| RsaNI | GTAC | 9 cut(s) | 1535, 1863, 2160, 3192, 3981, 4760, 5090, 5477, 5860 |
| RseI | CAYNNNNRTG | 5 cut(s) | 2194, 3697, 4254, 5301, 5883 |
| SacI | GAGCTC | 4 cut(s) | 21, 611, 1215, 1423 |
| SalI | GTCGAC | 1 cut(s) | 598 |
| SapI | GCTCTTC | 3 cut(s) | 1203, 2307, 5370 |
| SbfI | CCTGCAGG | 1 cut(s) | 1831 |
| ScaI | AGTACT | 3 cut(s) | 1864, 3193, 4761 |
| SdaI | CCTGCAGG | 1 cut(s) | 1831 |
| SfcI | CTRYAG | 9 cut(s) | 959, 1827, 1983, 2013, 2978, 3114, 3435, 4323, 4485 |
| SfoI | GGCGCC | 1 cut(s) | 93 |
| Sfr274I | CTCGAG | 1 cut(s) | 4734 |
| SfuI | TTCGAA | 1 cut(s) | 5577 |
| SgrAI | CRCCGGYG | 1 cut(s) | 553 |
| SgrDI | CGTCGACG | 1 cut(s) | 598 |
| SinI | GGWCC | 8 cut(s) | 1407, 1967, 2186, 3702, 4473, 5293, 5797, 6048 |
| SlaI | CTCGAG | 1 cut(s) | 4734 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 2194, 3697, 4254, 5301, 5883 |
| SmlI | CTYRAG | 3 cut(s) | 4010, 4700, 4734 |
| SmoI | CTYRAG | 3 cut(s) | 4010, 4700, 4734 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 1831 |
| SseBI | AGGCCT | 3 cut(s) | 1920, 3170, 3772 |
| SspDI | GGCGCC | 1 cut(s) | 91 |
| SspI | AATATT | 2 cut(s) | 3109, 3746 |
| SstI | GAGCTC | 4 cut(s) | 21, 611, 1215, 1423 |
| StuI | AGGCCT | 3 cut(s) | 1920, 3170, 3772 |
| StyI | CCWWGG | 3 cut(s) | 1348, 1410, 5025 |
| TatI | WGTACW | 4 cut(s) | 1862, 3191, 4759, 5089 |
| TauI | GCSGC | 9 cut(s) | 789, 803, 1660, 3131, 3137, 4990, 5349, 5585, 6005 |
| TfiI | GAWTC | 6 cut(s) | 1931, 2825, 2852, 3511, 4934, 5209 |
| TseFI | GTSAC | 8 cut(s) | 99, 124, 549, 556, 760, 2501, 2890, 4569 |
| Tsp45I | GTSAC | 8 cut(s) | 99, 124, 549, 556, 760, 2501, 2890, 4569 |
| Tth111I | GACNNNGTC | 3 cut(s) | 130, 386, 595 |
| VneI | GTGCAC | 1 cut(s) | 5757 |
| VpaK11BI | GGWCC | 8 cut(s) | 1407, 1967, 2186, 3702, 4473, 5293, 5797, 6048 |
| VspI | ATTAAT | 2 cut(s) | 941, 5412 |
| XbaI | TCTAGA | 1 cut(s) | 2522 |
| XceI | RCATGY | 2 cut(s) | 974, 1173 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2802 |
| XhoI | CTCGAG | 1 cut(s) | 4734 |
| XmiI | GTMKAC | 2 cut(s) | 599, 1584 |
| ZraI | GACGTC | 1 cut(s) | 385 |
| ZrmI | AGTACT | 3 cut(s) | 1864, 3193, 4761 |
| Zsp2I | ATGCAT | 2 cut(s) | 972, 3694 |