MD05G1100100.v1.1

DNA-directed DNA polymerase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0017237
Physical Location & Seq
Reverse (-)
20738306 .. 20739824
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1100100.v1.1.491

Sequence Viewer

Length: 681 bp
ATGGAACTATCATCCGTATCAATGATTTCAACTTCCAACCTCTCCATGCCAAATCCAACTGTTCATTTTCGCCGCACCATAAGCGTTTTAATCCGAACCAGAACCCGAATTGTTTCTTCATTACTCACCCCCACTACCCTTACCAAGAAAAGTAGAGAGGCCGGCACCGGACCCACAATGCCAACCATGGCTGAGATACTGGACTCATCAAAATCCCAGAAGCTGGACCTGCAGCTCCAAACTGTGGGACCCTTTTTCAGAATCACGGCCAGAAGCTTGGGGACGAAGAAAGAGCTCGGAAAGGCCGAGGGGCTCATAAGGGTCTGGCTGAAAGGGAGGATCCTTCACTTGGAGTCCATCAGACTCAAGAGAGAGACTCTGGGGATGGAGAAGTCGATATTTGGGATCGGTTTGTTTCTTGGAGCTGTTGCCGTTCGGTATGGATACGATTGCGGTTGCGGAACGGCTGAGCTGCTGGCTATCAATGACTCCGATATTTACCACCACAAGCTTGTGCGGTTCTATTCAAGAATCGGATTCAAGGCTGTACATGAAGTAACTGGTTCGACATTTGGAGACTTCGCCCATATGCTGGTCTGGGGAGGAATCGGGACCCGAATGGATGCCAGTGTTGAAGCGCTACTGATCAAATGGTGCACCAGGTTCAAATCTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.08

Weight (kDa)

10.05

Isoelectric Point (pI)

32.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017237)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04342
malus_domestica MD05G1100100.v1.1 MD05G1100400.v1.1
prunus_persica Prupe.8G144800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0253801
rosa_laevigata RLG00000014969
rosa_roxburghii Rroxscaffold_7G00211510
rosa_rugosa Rorug05G0547300
rosa_samantha Rh6AG062900 Rh6BG056200 Rh6CG056300 Rh6DG054100
rosa_wichuraiana Rw6G005600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 237
AccB1I GGYRCC 1 cut(s) 164
AccB7I CCANNNNNTGG 3 cut(s) 223, 244, 592
AciI CCGC 4 cut(s) 73, 453, 459, 517
AclWI GGATC 3 cut(s) 334, 347, 413
AcoI YGGCCR 1 cut(s) 267
AfaI GTAC 1 cut(s) 549
AfeI AGCGCT 1 cut(s) 639
AfiI CCNNNNNNNGG 4 cut(s) 223, 244, 349, 592
AgsI TTSAA 5 cut(s) 30, 528, 541, 635, 667
AjnI CCWGG 1 cut(s) 659
AluBI AGCT 7 cut(s) 223, 235, 276, 295, 425, 472, 511
AluI AGCT 7 cut(s) 223, 235, 276, 295, 425, 472, 511
Alw21I GWGCWC 2 cut(s) 297, 659
Alw26I GTCTC 2 cut(s) 368, 570
Alw44I GTGCAC 1 cut(s) 655
AlwI GGATC 3 cut(s) 334, 347, 413
AlwNI CAGNNNCTG 1 cut(s) 223
Aor51HI AGCGCT 1 cut(s) 639
AoxI GGCC 3 cut(s) 159, 267, 303
ApaLI GTGCAC 1 cut(s) 655
ApeKI GCWGC 2 cut(s) 232, 472
Asp700I GAANNNNTTC 1 cut(s) 112
AspLEI GCGC 1 cut(s) 640
AspS9I GGNCC 4 cut(s) 170, 226, 248, 612
AsuHPI GGTGA 1 cut(s) 118
AvaII GGWCC 4 cut(s) 170, 226, 248, 612
BaeGI GKGCMC 1 cut(s) 659
BamHI GGATCC 1 cut(s) 339
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 2 cut(s) 297, 315
Bbv12I GWGCWC 2 cut(s) 297, 659
BbvI GCAGC 2 cut(s) 244, 459
BccI CCATC 2 cut(s) 365, 379
BceAI ACGGC 3 cut(s) 282, 416, 480
BciT130I CCWGG 1 cut(s) 661
BciVI GTATCC 1 cut(s) 437
BclI TGATCA 1 cut(s) 645
BcoDI GTCTC 2 cut(s) 368, 570
BfmI CTRYAG 1 cut(s) 230
BfoI RGCGCY 1 cut(s) 641
BfuAI ACCTGC 1 cut(s) 237
BfuI GTATCC 1 cut(s) 437
BisI GCNGC 3 cut(s) 73, 233, 473
BlpI GCTNAGC 1 cut(s) 468
BlsI GCNGC 3 cut(s) 74, 234, 474
Bme1390I CCNGG 1 cut(s) 661
Bme18I GGWCC 4 cut(s) 170, 226, 248, 612
BmgT120I GGNCC 4 cut(s) 170, 226, 248, 612
BmiI GGNNCC 7 cut(s) 166, 172, 249, 250, 341, 613, 614
BmrFI CCNGG 1 cut(s) 661
BmsI GCATC 1 cut(s) 613
BplI GAGNNNNNCTC 2 cut(s) 361, 393
Bpu1102I GCTNAGC 1 cut(s) 468
BpuEI CTTGAG 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 186, 306
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 244, 349, 592
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 3 cut(s) 204, 565, 627
BseBI CCWGG 1 cut(s) 661
BseDI CCNNGG 2 cut(s) 186, 306
BseGI GGATG 3 cut(s) 11, 390, 628
BseLI CCNNNNNNNGG 4 cut(s) 223, 244, 349, 592
BseMII CTCAG 2 cut(s) 183, 459
BseNI ACTGG 3 cut(s) 204, 565, 627
BseSI GKGCMC 1 cut(s) 659
BseXI GCAGC 2 cut(s) 244, 459
BshFI GGCC 3 cut(s) 161, 269, 305
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 2 cut(s) 297, 659
BsiSI CCGG 2 cut(s) 162, 168
BslFI GGGAC 3 cut(s) 261, 295, 625
BslI CCNNNNNNNGG 4 cut(s) 223, 244, 349, 592
BsmAI GTCTC 2 cut(s) 368, 570
BsmFI GGGAC 3 cut(s) 261, 295, 625
BsnI GGCC 3 cut(s) 161, 269, 305
Bsp1286I GDGCHC 3 cut(s) 297, 315, 659
Bsp1407I TGTACA 1 cut(s) 547
Bsp143I GATC 3 cut(s) 339, 405, 645
Bsp1720I GCTNAGC 1 cut(s) 468
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 4 cut(s) 73, 453, 459, 517
BspANI GGCC 3 cut(s) 161, 269, 305
BspCNI CTCAG 2 cut(s) 184, 460
BspLI GGNNCC 7 cut(s) 166, 172, 249, 250, 341, 613, 614
BspMAI CTGCAG 1 cut(s) 234
BspMI ACCTGC 1 cut(s) 237
BspPI GGATC 3 cut(s) 334, 347, 413
BspT107I GGYRCC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 161
BsrGI TGTACA 1 cut(s) 547
BsrI ACTGG 3 cut(s) 204, 565, 627
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 186, 306
BssMI GATC 3 cut(s) 339, 405, 645
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 661
Bst4CI ACNGT 2 cut(s) 61, 244
BstAUI TGTACA 1 cut(s) 547
BstC8I GCNNGC 2 cut(s) 163, 477
BstDEI CTNAG 2 cut(s) 192, 468
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 3 cut(s) 11, 390, 628
BstH2I RGCGCY 1 cut(s) 641
BstHHI GCGC 1 cut(s) 640
BstKTI GATC 3 cut(s) 342, 408, 648
BstMAI GTCTC 2 cut(s) 368, 570
BstMBI GATC 3 cut(s) 339, 405, 645
BstMWI GCNNNNNNNGC 2 cut(s) 81, 229
BstNI CCWGG 1 cut(s) 661
BstSCI CCNGG 1 cut(s) 659
BstSFI CTRYAG 1 cut(s) 230
BstSLI GKGCMC 1 cut(s) 659
BstV1I GCAGC 2 cut(s) 244, 459
BstX2I RGATCY 1 cut(s) 339
BstXI CCANNNNNNTGG 1 cut(s) 277
BstYI RGATCY 1 cut(s) 339
BsuI GTATCC 1 cut(s) 437
BsuRI GGCC 3 cut(s) 161, 269, 305
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 3 cut(s) 11, 390, 628
BtsIMutI CAGTG 1 cut(s) 634
BveI ACCTGC 1 cut(s) 237
Cac8I GCNNGC 2 cut(s) 163, 477
CaiI CAGNNNCTG 1 cut(s) 223
CfoI GCGC 1 cut(s) 640
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 4 cut(s) 170, 226, 248, 612
CsiI ACCWGGT 1 cut(s) 659
Csp6I GTAC 1 cut(s) 548
CviAII CATG 3 cut(s) 46, 187, 551
CviQI GTAC 1 cut(s) 548
DdeI CTNAG 2 cut(s) 192, 468
DpnI GATC 3 cut(s) 341, 407, 647
DpnII GATC 3 cut(s) 339, 405, 645
EaeI YGGCCR 1 cut(s) 267
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 2 cut(s) 297, 315
Eco47I GGWCC 4 cut(s) 170, 226, 248, 612
Eco47III AGCGCT 1 cut(s) 639
Eco53kI GAGCTC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 295
EcoO109I RGGNCCY 2 cut(s) 248, 612
EcoRII CCWGG 1 cut(s) 659
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 2 cut(s) 297, 315
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 3 cut(s) 49, 190, 554
FaiI YATR 8 cut(s) 47, 80, 188, 317, 441, 552, 588, 590
FaqI GGGAC 3 cut(s) 261, 295, 625
FatI CATG 3 cut(s) 45, 186, 550
FauNDI CATATG 1 cut(s) 588
FbaI TGATCA 1 cut(s) 645
Fnu4HI GCNGC 3 cut(s) 73, 233, 473
FokI GGATG 2 cut(s) 397, 635
FriOI GRGCYC 2 cut(s) 297, 315
Fsp4HI GCNGC 3 cut(s) 73, 233, 473
GlaI GCGC 1 cut(s) 639
GluI GCNGC 3 cut(s) 73, 233, 473
HaeII RGCGCY 1 cut(s) 641
HaeIII GGCC 3 cut(s) 161, 269, 305
HapII CCGG 2 cut(s) 162, 168
HhaI GCGC 1 cut(s) 640
Hin1II CATG 3 cut(s) 49, 190, 554
Hin6I GCGC 1 cut(s) 638
HinP1I GCGC 1 cut(s) 638
HindIII AAGCTT 2 cut(s) 274, 509
HinfI GANTC 9 cut(s) 203, 261, 353, 363, 376, 488, 531, 537, 606
HpaII CCGG 2 cut(s) 162, 168
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 1 cut(s) 657
Hpy188I TCNGA 6 cut(s) 95, 260, 299, 362, 493, 536
Hpy188III TCNNGA 3 cut(s) 367, 528, 610
Hpy8I GTNNAC 1 cut(s) 657
HpyAV CCTTC 1 cut(s) 353
HpyCH4III ACNGT 2 cut(s) 61, 244
HpyCH4V TGCA 2 cut(s) 232, 657
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 229
HpyF3I CTNAG 2 cut(s) 192, 468
Hsp92II CATG 3 cut(s) 49, 190, 554
HspAI GCGC 1 cut(s) 638
KflI GGGWCCC 2 cut(s) 248, 612
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 645
Kzo9I GATC 3 cut(s) 339, 405, 645
LmnI GCTCC 2 cut(s) 240, 422
Lsp1109I GCAGC 2 cut(s) 244, 459
LweI GCATC 1 cut(s) 613
MabI ACCWGGT 1 cut(s) 659
MaeIII GTNAC 1 cut(s) 556
MalI GATC 3 cut(s) 341, 407, 647
MboI GATC 3 cut(s) 339, 405, 645
MboII GAAGA 2 cut(s) 108, 298
MflI RGATCY 1 cut(s) 339
MhlI GDGCHC 3 cut(s) 297, 315, 659
MluCI AATT 1 cut(s) 108
MlyI GAGTC 5 cut(s) 197, 357, 362, 370, 482
MmeI TCCRAC 2 cut(s) 60, 80
MnlI CCTC 5 cut(s) 50, 151, 301, 330, 596
MroNI GCCGGC 1 cut(s) 161
MroXI GAANNNNTTC 1 cut(s) 112
MseI TTAA 1 cut(s) 89
MspI CCGG 2 cut(s) 162, 168
MspR9I CCNGG 1 cut(s) 661
MvaI CCWGG 1 cut(s) 661
MwoI GCNNNNNNNGC 2 cut(s) 81, 229
NaeI GCCGGC 1 cut(s) 163
NcoI CCATGG 1 cut(s) 186
NdeI CATATG 1 cut(s) 588
NdeII GATC 3 cut(s) 339, 405, 645
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 3 cut(s) 49, 190, 554
NlaIV GGNNCC 7 cut(s) 166, 172, 249, 250, 341, 613, 614
NmeAIII GCCGAG 1 cut(s) 331
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PdiI GCCGGC 1 cut(s) 163
PdmI GAANNNNTTC 1 cut(s) 112
PfeI GAWTC 4 cut(s) 261, 531, 537, 606
PflMI CCANNNNNTGG 3 cut(s) 223, 244, 592
PkrI GCNGC 3 cut(s) 74, 234, 474
PleI GAGTC 5 cut(s) 197, 357, 361, 370, 482
PpsI GAGTC 5 cut(s) 197, 357, 361, 370, 482
PpuMI RGGWCCY 2 cut(s) 248, 612
Psp124BI GAGCTC 1 cut(s) 297
Psp5II RGGWCCY 2 cut(s) 248, 612
Psp6I CCWGG 1 cut(s) 659
PspGI CCWGG 1 cut(s) 659
PspN4I GGNNCC 7 cut(s) 166, 172, 249, 250, 341, 613, 614
PspPI GGNCC 4 cut(s) 170, 226, 248, 612
PspPPI RGGWCCY 2 cut(s) 248, 612
PstI CTGCAG 1 cut(s) 234
PstNI CAGNNNCTG 1 cut(s) 223
PsuI RGATCY 1 cut(s) 339
RsaI GTAC 1 cut(s) 549
RsaNI GTAC 1 cut(s) 548
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 89
SatI GCNGC 3 cut(s) 73, 233, 473
Sau3AI GATC 3 cut(s) 339, 405, 645
Sau96I GGNCC 4 cut(s) 170, 226, 248, 612
SchI GAGTC 5 cut(s) 197, 357, 362, 370, 482
ScrFI CCNGG 1 cut(s) 661
SduI GDGCHC 3 cut(s) 297, 315, 659
SexAI ACCWGGT 1 cut(s) 659
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 1 cut(s) 230
SinI GGWCC 4 cut(s) 170, 226, 248, 612
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 1 cut(s) 108
SsiI CCGC 4 cut(s) 73, 453, 459, 517
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 659
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 2 cut(s) 61, 244
TaqI TCGA 2 cut(s) 395, 566
TasI AATT 1 cut(s) 108
TatI WGTACW 1 cut(s) 547
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 4 cut(s) 261, 531, 537, 606
Tru1I TTAA 1 cut(s) 89
Tru9I TTAA 1 cut(s) 89
TscAI CASTG 1 cut(s) 634
TseI GCWGC 2 cut(s) 232, 472
TspDTI ATGAA 3 cut(s) 53, 108, 567
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 1 cut(s) 634
Van91I CCANNNNNTGG 3 cut(s) 223, 244, 592
VneI GTGCAC 1 cut(s) 655
VpaK11BI GGWCC 4 cut(s) 170, 226, 248, 612
XmnI GAANNNNTTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.