MD05G1110600.v1.1

Protein trichome birefringence-like 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0018513
Physical Location & Seq
Reverse (-)
22749010 .. 22750980
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1110600.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGTAGCTTTGATGTTCGTCTGTATGTGGTCAGCCGATGCTAGTGGTGGGGTGCTGGAATATCTTGGATACAAGGAGGGTTATAGAGTTGATGTTGACATTCCAGACGGCACATGGGCAGAAGCTCTAAGCTTTCATGACATTCTGATCTTTAATACAGGGCACTCGTATGTAATGTTCATGGAGAAGAGAACGAAAGCAGGTGCAATCAAATTTTTTCGTACTCAGTCACCTAGACATTTTGAAGGGGGTGACTGGGACCAAGGTGGCTCTTGTAATCGGGTAAATCCTTTATCGGTAGAGCAGGTCGAAGATCTCTTCTCAGTGAAGAATAATGGTACAAACGTGGAGGCACGCCTGGTGAATCAACATCTCTACAAGTCCCTTAAAGGATCAAGTTTCCAGATTTTGAACATAACCCACATGAGCGAGTTCAGAGCAGATGGCCATCCATCCACAGCCAGTGGGAAGAAGCACGACGACTGCATGCACTGGTGCATGCCAGGTTAA

Protein Analysis

170

Amino Acids

18.99

Weight (kDa)

6.03

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 67 - 169 7.6e-27 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018513)

Species Orthologous Gene IDs
malus_domestica MD05G1110600.v1.1
rosa_chinensis RchiOBHm_Chr6g0247311
rosa_roxburghii Rroxscaffold_180G00433560
rosa_samantha Rh6AG039100 Rh6AG039300 Rh6AG040100 Rh6BG034600 Rh6CG034500 Rh6DG032200
rosa_wichuraiana Rw0G020480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 191
Acc36I ACCTGC 2 cut(s) 191, 295
AclWI GGATC 1 cut(s) 400
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 1 cut(s) 212
AfaI GTAC 2 cut(s) 223, 340
AgsI TTSAA 2 cut(s) 245, 412
AjnI CCWGG 2 cut(s) 357, 502
AluBI AGCT 3 cut(s) 8, 125, 132
AluI AGCT 3 cut(s) 8, 125, 132
AlwI GGATC 1 cut(s) 400
AoxI GGCC 1 cut(s) 445
ApoI RAATTY 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 259
AsuHPI GGTGA 3 cut(s) 222, 263, 373
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 165
BaeI ACNNNNGTAYC 2 cut(s) 330, 363
BalI TGGCCA 1 cut(s) 447
BccI CCATC 3 cut(s) 437, 456, 460
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 2 cut(s) 359, 504
BciVI GTATCC 1 cut(s) 62
BfaI CTAG 2 cut(s) 42, 234
BfuAI ACCTGC 2 cut(s) 191, 295
BfuI GTATCC 1 cut(s) 62
BglII AGATCT 1 cut(s) 313
Bme1390I CCNGG 2 cut(s) 359, 504
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 1 cut(s) 260
BmrFI CCNGG 2 cut(s) 359, 504
BmrI ACTGGG 1 cut(s) 265
BmsI GCATC 1 cut(s) 28
BmuI ACTGGG 1 cut(s) 265
BsaBI GATNNNNATC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 262
Bse1I ACTGG 3 cut(s) 260, 462, 497
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 2 cut(s) 359, 504
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 2 cut(s) 448, 452
BseJI GATNNNNATC 1 cut(s) 447
BseMII CTCAG 2 cut(s) 239, 336
BseNI ACTGG 3 cut(s) 260, 462, 497
BseSI GKGCMC 1 cut(s) 165
BshFI GGCC 1 cut(s) 447
BslFI GGGAC 2 cut(s) 272, 367
BsmFI GGGAC 2 cut(s) 272, 367
BsnI GGCC 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 165
Bsp143I GATC 3 cut(s) 147, 313, 392
BspANI GGCC 1 cut(s) 447
BspCNI CTCAG 2 cut(s) 238, 335
BspHI TCATGA 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 260
BspMI ACCTGC 2 cut(s) 191, 295
BspPI GGATC 1 cut(s) 400
BsrI ACTGG 3 cut(s) 260, 462, 497
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 3 cut(s) 147, 313, 392
BssT1I CCWWGG 1 cut(s) 262
Bst2UI CCWGG 2 cut(s) 359, 504
Bst6I CTCTTC 2 cut(s) 182, 323
BstC8I GCNNGC 3 cut(s) 355, 488, 500
BstDEI CTNAG 3 cut(s) 128, 225, 322
BstF5I GGATG 2 cut(s) 448, 452
BstKTI GATC 3 cut(s) 150, 316, 395
BstMBI GATC 3 cut(s) 147, 313, 392
BstNI CCWGG 2 cut(s) 359, 504
BstNSI RCATGY 2 cut(s) 490, 502
BstSCI CCNGG 2 cut(s) 357, 502
BstSLI GKGCMC 1 cut(s) 165
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BsuI GTATCC 1 cut(s) 62
BsuRI GGCC 1 cut(s) 447
BtsCI GGATG 2 cut(s) 448, 452
BtsIMutI CAGTG 3 cut(s) 330, 469, 490
BveI ACCTGC 2 cut(s) 191, 295
Cac8I GCNNGC 3 cut(s) 355, 488, 500
CciI TCATGA 1 cut(s) 136
Cfr13I GGNCC 1 cut(s) 259
Csp6I GTAC 2 cut(s) 222, 339
CviAII CATG 6 cut(s) 114, 137, 181, 424, 487, 499
CviJI RGCY 7 cut(s) 8, 35, 125, 132, 270, 447, 461
CviKI_1 RGCY 7 cut(s) 8, 35, 125, 132, 270, 447, 461
CviQI GTAC 2 cut(s) 222, 339
DdeI CTNAG 3 cut(s) 128, 225, 322
DpnI GATC 3 cut(s) 149, 315, 394
DpnII GATC 3 cut(s) 147, 313, 392
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 2 cut(s) 182, 323
EarI CTCTTC 2 cut(s) 182, 323
Eco130I CCWWGG 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 259
EcoRII CCWGG 2 cut(s) 357, 502
EcoT14I CCWWGG 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 262
FaeI CATG 6 cut(s) 117, 140, 184, 427, 490, 502
FaqI GGGAC 2 cut(s) 272, 367
FatI CATG 6 cut(s) 113, 136, 180, 423, 486, 498
FokI GGATG 2 cut(s) 435, 439
FspBI CTAG 2 cut(s) 42, 234
HaeIII GGCC 1 cut(s) 447
Hin1II CATG 6 cut(s) 117, 140, 184, 427, 490, 502
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 1 cut(s) 130
HinfI GANTC 1 cut(s) 364
HphI GGTGA 3 cut(s) 222, 263, 373
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 2 cut(s) 147, 437
Hpy188III TCNNGA 3 cut(s) 104, 137, 403
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 1 cut(s) 482
HpyAV CCTTC 1 cut(s) 239
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 4 cut(s) 206, 486, 490, 498
HpyF3I CTNAG 3 cut(s) 128, 225, 322
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 6 cut(s) 117, 140, 184, 427, 490, 502
Kzo9I GATC 3 cut(s) 147, 313, 392
LweI GCATC 1 cut(s) 28
MaeI CTAG 2 cut(s) 42, 234
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 2 cut(s) 228, 251
MalI GATC 3 cut(s) 149, 315, 394
MboI GATC 3 cut(s) 147, 313, 392
MboII GAAGA 5 cut(s) 199, 310, 323, 340, 481
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 165
MlsI TGGCCA 1 cut(s) 447
MluCI AATT 1 cut(s) 212
MluNI TGGCCA 1 cut(s) 447
MnlI CCTC 2 cut(s) 70, 343
Mox20I TGGCCA 1 cut(s) 447
MscI TGGCCA 1 cut(s) 447
MseI TTAA 3 cut(s) 153, 387, 508
MslI CAYNNNNRTG 1 cut(s) 168
Msp20I TGGCCA 1 cut(s) 447
MspR9I CCNGG 2 cut(s) 359, 504
MvaI CCWGG 2 cut(s) 359, 504
NdeII GATC 3 cut(s) 147, 313, 392
NlaIII CATG 6 cut(s) 117, 140, 184, 427, 490, 502
NlaIV GGNNCC 1 cut(s) 260
NmuCI GTSAC 2 cut(s) 228, 251
NspI RCATGY 2 cut(s) 490, 502
PaeI GCATGC 2 cut(s) 490, 502
PagI TCATGA 1 cut(s) 136
PaqCI CACCTGC 1 cut(s) 191
PfeI GAWTC 1 cut(s) 364
Psp6I CCWGG 2 cut(s) 357, 502
PspGI CCWGG 2 cut(s) 357, 502
PspN4I GGNNCC 1 cut(s) 260
PspPI GGNCC 1 cut(s) 259
PsrI GAACNNNNNNTAC 2 cut(s) 161, 193
PsuI RGATCY 1 cut(s) 313
RsaI GTAC 2 cut(s) 223, 340
RsaNI GTAC 2 cut(s) 222, 339
RseI CAYNNNNRTG 1 cut(s) 168
SaqAI TTAA 3 cut(s) 153, 387, 508
Sau3AI GATC 3 cut(s) 147, 313, 392
Sau96I GGNCC 1 cut(s) 259
ScrFI CCNGG 2 cut(s) 359, 504
SduI GDGCHC 1 cut(s) 165
SetI ASST 9 cut(s) 10, 127, 134, 205, 235, 268, 309, 348, 508
SfaNI GCATC 1 cut(s) 28
SinI GGWCC 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 168
SphI GCATGC 2 cut(s) 490, 502
Sse9I AATT 1 cut(s) 212
SspMI CTAG 2 cut(s) 42, 234
StyD4I CCNGG 2 cut(s) 357, 502
StyI CCWWGG 1 cut(s) 262
TaiI ACGT 1 cut(s) 348
TaqI TCGA 1 cut(s) 309
TasI AATT 1 cut(s) 212
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 3 cut(s) 153, 387, 508
Tru9I TTAA 3 cut(s) 153, 387, 508
TscAI CASTG 3 cut(s) 330, 469, 497
TseFI GTSAC 2 cut(s) 228, 251
Tsp45I GTSAC 2 cut(s) 228, 251
TspDTI ATGAA 2 cut(s) 125, 169
TspRI CASTG 3 cut(s) 330, 469, 497
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 1 cut(s) 212
XceI RCATGY 2 cut(s) 490, 502
XcmI CCANNNNNNNNNTGG 1 cut(s) 111
XspI CTAG 2 cut(s) 42, 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.