Rh6AG040100

Protein trichome birefringence-like 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0018513
Physical Location & Seq
Forward (+)
5532950 .. 5534525
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG040100.1

Sequence Viewer

Length: 684 bp
ATGGTTAGTGACAATTCTAATGGTGGTGTGCTGGAATCTGTTGGATATAAAGAGGGTTATAGAGTTGATGTTGACATTCCGGTTGACACATGGGCAGAATCTCTAAGCTTTCATGACATTCTGATCTTCAACACAGGGCACTGGTATTGGGCTCCTGCAAAGTTTGACCCTATGAAGTCGCCTCTGCTTTTTTTTGAAAATGATCAGCCTGTGGTTCCTCCAGTACTTCCTGATGTTGGCCTCGATATGGTTATGAAACACATGGTAATGTTTGTGGAGAAGAGACTGAAGCCTGGTGCAATCAAATTCTTTCGTACTCAATCACCTATACATTTTGAAGGAGGTGACTGGAACCAAGTTGGTTCTTGTAACCGGCAGAACCCTTTGTTGCCAGAGCAGGTTGAAGAACTCTTCTCATTGAAGAATAATGGAACAAATGTAGAGGCACGACTCGTGAATCAACACCTTTACAAGGCCCTTGAGGGGTCAGGTTTCCATATTTTGGACATAACCCATCTGAGTGAGTTCAGAGCAGATGCCCATCCATCAGCAGCTGGCGGGAAGAAGCACGATGATTGTATGCACTGGTGCTTGCCAGGAATAACAGATACTTGGAATGACTTGTTCATAGCACAGTTAAATAATTATTTCATTCCACTGAAATTCTCTTGCGCAAGTCGTTGA

Protein Analysis

227

Amino Acids

25.72

Weight (kDa)

5.5

Isoelectric Point (pI)

30.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 17 - 211 8.9e-45 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018513)

Species Orthologous Gene IDs
malus_domestica MD05G1110600.v1.1
rosa_chinensis RchiOBHm_Chr6g0247311
rosa_roxburghii Rroxscaffold_180G00433560
rosa_samantha Rh6AG039100 Rh6AG039300 Rh6AG040100 Rh6BG034600 Rh6CG034500 Rh6DG032200
rosa_wichuraiana Rw0G020480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 673
Acc36I ACCTGC 1 cut(s) 388
AccB7I CCANNNNNTGG 1 cut(s) 502
AciI CCGC 1 cut(s) 558
AcsI RAATTY 2 cut(s) 305, 662
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 2 cut(s) 225, 316
AfiI CCNNNNNNNGG 5 cut(s) 236, 247, 472, 483, 502
AgsI TTSAA 5 cut(s) 130, 197, 338, 404, 421
AjnI CCWGG 2 cut(s) 292, 595
AluBI AGCT 2 cut(s) 108, 554
AluI AGCT 2 cut(s) 108, 554
Alw26I GTCTC 1 cut(s) 277
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 2 cut(s) 238, 474
ApeKI GCWGC 1 cut(s) 551
ApoI RAATTY 2 cut(s) 305, 662
AspLEI GCGC 1 cut(s) 674
AspS9I GGNCC 1 cut(s) 475
AsuHPI GGTGA 2 cut(s) 315, 356
BaeGI GKGCMC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 154
BauI CACGAG 1 cut(s) 452
BbvI GCAGC 1 cut(s) 563
BccI CCATC 3 cut(s) 522, 549, 553
BciT130I CCWGG 2 cut(s) 294, 597
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 277
BfuAI ACCTGC 1 cut(s) 388
BisI GCNGC 1 cut(s) 552
BlsI GCNGC 1 cut(s) 553
BmcAI AGTACT 1 cut(s) 225
Bme1390I CCNGG 2 cut(s) 294, 597
BmgT120I GGNCC 1 cut(s) 475
BmiI GGNNCC 3 cut(s) 153, 216, 353
BmrFI CCNGG 2 cut(s) 294, 597
BmsI GCATC 1 cut(s) 526
BpmI CTGGAG 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 500
BsaBI GATNNNNATC 1 cut(s) 540
BsaWI WCCGGW 1 cut(s) 79
Bsc4I CCNNNNNNNGG 5 cut(s) 236, 247, 472, 483, 502
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 4 cut(s) 146, 221, 353, 590
Bse8I GATNNNNATC 1 cut(s) 540
BseBI CCWGG 2 cut(s) 294, 597
BseGI GGATG 1 cut(s) 541
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 5 cut(s) 236, 247, 472, 483, 502
BseMII CTCAG 1 cut(s) 509
BseNI ACTGG 4 cut(s) 146, 221, 353, 590
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 1 cut(s) 563
BshFI GGCC 2 cut(s) 240, 476
BsiSI CCGG 2 cut(s) 80, 373
BslI CCNNNNNNNGG 5 cut(s) 236, 247, 472, 483, 502
BsmAI GTCTC 1 cut(s) 277
BsnI GGCC 2 cut(s) 240, 476
Bsp1286I GDGCHC 2 cut(s) 141, 154
Bsp143I GATC 2 cut(s) 123, 202
BspACI CCGC 1 cut(s) 558
BspANI GGCC 2 cut(s) 240, 476
BspCNI CTCAG 1 cut(s) 510
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 3 cut(s) 153, 216, 353
BspMI ACCTGC 1 cut(s) 388
BsrFI RCCGGY 1 cut(s) 372
BsrI ACTGG 4 cut(s) 146, 221, 353, 590
BssAI RCCGGY 1 cut(s) 372
BssMI GATC 2 cut(s) 123, 202
BssSI CACGAG 1 cut(s) 452
Bst2BI CACGAG 1 cut(s) 452
Bst2UI CCWGG 2 cut(s) 294, 597
Bst4CI ACNGT 1 cut(s) 636
Bst6I CTCTTC 2 cut(s) 275, 416
BstC8I GCNNGC 2 cut(s) 556, 593
BstDEI CTNAG 2 cut(s) 104, 518
BstENI CCTNNNNNAGG 1 cut(s) 470
BstF5I GGATG 1 cut(s) 541
BstHHI GCGC 1 cut(s) 674
BstKTI GATC 2 cut(s) 126, 205
BstMAI GTCTC 1 cut(s) 277
BstMBI GATC 2 cut(s) 123, 202
BstNI CCWGG 2 cut(s) 294, 597
BstSCI CCNGG 2 cut(s) 292, 595
BstSLI GKGCMC 1 cut(s) 141
BstV1I GCAGC 1 cut(s) 563
BsuRI GGCC 2 cut(s) 240, 476
BtsCI GGATG 1 cut(s) 541
BtsIMutI CAGTG 3 cut(s) 139, 583, 656
BveI ACCTGC 1 cut(s) 388
Cac8I GCNNGC 2 cut(s) 556, 593
CaiI CAGNNNCTG 1 cut(s) 554
CciI TCATGA 1 cut(s) 112
CfoI GCGC 1 cut(s) 674
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 1 cut(s) 475
Csp6I GTAC 2 cut(s) 224, 315
CviAII CATG 3 cut(s) 90, 113, 262
CviJI RGCY 7 cut(s) 108, 152, 208, 240, 292, 476, 554
CviKI_1 RGCY 7 cut(s) 108, 152, 208, 240, 292, 476, 554
CviQI GTAC 2 cut(s) 224, 315
DdeI CTNAG 2 cut(s) 104, 518
DpnI GATC 2 cut(s) 125, 204
DpnII GATC 2 cut(s) 123, 202
Eam1104I CTCTTC 2 cut(s) 275, 416
EarI CTCTTC 2 cut(s) 275, 416
Eco24I GRGCYC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 308
EcoNI CCTNNNNNAGG 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 475
EcoRII CCWGG 2 cut(s) 292, 595
EcoT38I GRGCYC 1 cut(s) 154
FaeI CATG 3 cut(s) 93, 116, 265
FatI CATG 3 cut(s) 89, 112, 261
FauI CCCGC 1 cut(s) 551
FbaI TGATCA 1 cut(s) 202
Fnu4HI GCNGC 1 cut(s) 552
FokI GGATG 1 cut(s) 528
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 1 cut(s) 552
FspI TGCGCA 1 cut(s) 673
GlaI GCGC 1 cut(s) 673
GluI GCNGC 1 cut(s) 552
GsuI CTGGAG 1 cut(s) 204
HaeIII GGCC 2 cut(s) 240, 476
HapII CCGG 2 cut(s) 80, 373
HhaI GCGC 1 cut(s) 674
Hin1II CATG 3 cut(s) 93, 116, 265
Hin6I GCGC 1 cut(s) 672
HinP1I GCGC 1 cut(s) 672
HincII GTYRAC 2 cut(s) 73, 85
HindII GTYRAC 2 cut(s) 73, 85
HindIII AAGCTT 1 cut(s) 106
HinfI GANTC 4 cut(s) 35, 98, 450, 457
HpaII CCGG 2 cut(s) 80, 373
HphI GGTGA 2 cut(s) 315, 356
Hpy166II GTNNAC 2 cut(s) 73, 85
Hpy188I TCNGA 3 cut(s) 123, 519, 530
Hpy188III TCNNGA 3 cut(s) 113, 230, 454
Hpy8I GTNNAC 2 cut(s) 73, 85
HpyAV CCTTC 1 cut(s) 332
HpyCH4III ACNGT 1 cut(s) 636
HpyCH4V TGCA 3 cut(s) 158, 299, 583
HpyF3I CTNAG 2 cut(s) 104, 518
Hsp92II CATG 3 cut(s) 93, 116, 265
HspAI GCGC 1 cut(s) 672
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 2 cut(s) 123, 202
LmnI GCTCC 1 cut(s) 157
Lsp1109I GCAGC 1 cut(s) 563
LweI GCATC 1 cut(s) 526
MaeIII GTNAC 3 cut(s) 8, 344, 368
MalI GATC 2 cut(s) 125, 204
MboI GATC 2 cut(s) 123, 202
MboII GAAGA 6 cut(s) 118, 292, 403, 416, 433, 574
MhlI GDGCHC 2 cut(s) 141, 154
MluCI AATT 4 cut(s) 13, 305, 643, 662
MlyI GAGTC 1 cut(s) 444
MmeI TCCRAC 1 cut(s) 22
MnlI CCTC 7 cut(s) 46, 192, 228, 251, 335, 436, 475
MseI TTAA 1 cut(s) 638
MslI CAYNNNNRTG 2 cut(s) 266, 519
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 2 cut(s) 80, 373
MspR9I CCNGG 2 cut(s) 294, 597
MvaI CCWGG 2 cut(s) 294, 597
NdeII GATC 2 cut(s) 123, 202
NlaIII CATG 3 cut(s) 93, 116, 265
NlaIV GGNNCC 3 cut(s) 153, 216, 353
NmuCI GTSAC 2 cut(s) 8, 344
NsbI TGCGCA 1 cut(s) 673
PagI TCATGA 1 cut(s) 112
PfeI GAWTC 3 cut(s) 35, 98, 457
PflMI CCANNNNNTGG 1 cut(s) 502
PkrI GCNGC 1 cut(s) 553
PleI GAGTC 1 cut(s) 444
PpsI GAGTC 1 cut(s) 444
Psp6I CCWGG 2 cut(s) 292, 595
PspGI CCWGG 2 cut(s) 292, 595
PspN4I GGNNCC 3 cut(s) 153, 216, 353
PspPI GGNCC 1 cut(s) 475
PstNI CAGNNNCTG 1 cut(s) 554
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 2 cut(s) 225, 316
RsaNI GTAC 2 cut(s) 224, 315
RseI CAYNNNNRTG 2 cut(s) 266, 519
SaqAI TTAA 1 cut(s) 638
SatI GCNGC 1 cut(s) 552
Sau3AI GATC 2 cut(s) 123, 202
Sau96I GGNCC 1 cut(s) 475
ScaI AGTACT 1 cut(s) 225
SchI GAGTC 1 cut(s) 444
ScrFI CCNGG 2 cut(s) 294, 597
SduI GDGCHC 2 cut(s) 141, 154
SetI ASST 7 cut(s) 110, 328, 346, 402, 468, 493, 556
SfaNI GCATC 1 cut(s) 526
SmiMI CAYNNNNRTG 2 cut(s) 266, 519
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 4 cut(s) 13, 305, 643, 662
SsiI CCGC 1 cut(s) 558
StyD4I CCNGG 2 cut(s) 292, 595
TaaI ACNGT 1 cut(s) 636
TaqI TCGA 1 cut(s) 243
TasI AATT 4 cut(s) 13, 305, 643, 662
TatI WGTACW 1 cut(s) 223
TfiI GAWTC 3 cut(s) 35, 98, 457
Tru1I TTAA 1 cut(s) 638
Tru9I TTAA 1 cut(s) 638
TscAI CASTG 3 cut(s) 146, 590, 663
TseFI GTSAC 2 cut(s) 8, 344
TseI GCWGC 1 cut(s) 551
Tsp45I GTSAC 2 cut(s) 8, 344
TspDTI ATGAA 5 cut(s) 101, 188, 269, 616, 640
TspRI CASTG 3 cut(s) 146, 590, 663
Van91I CCANNNNNTGG 1 cut(s) 502
XagI CCTNNNNNAGG 1 cut(s) 470
XapI RAATTY 2 cut(s) 305, 662
ZrmI AGTACT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.