Rh6AG039300

Protein trichome birefringence-like 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0018513
Physical Location & Seq
Forward (+)
5444323 .. 5450554
6232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG039300.1

Sequence Viewer

Length: 486 bp
ATGAAGTCGCCTGTGCTTTTCTTTGAAAATGGTCAGCCTGTGGTTCCTCCAGTACATCCTGATGTTGGCCTCGATATGGTTATGAAACACATGGTAATGTTTGTGGAGAAGAGACTGAAACCTGGTGCAATCAAATTCTTTCGTACTCAATCACCTAGACATTTTGAAGGAGGTGACTGGAACCAAGGTGGTTCTTGTAACCGGCAAAACCCTTTGTTGCTAGAGCAGGTTGAGGAACTCTTCTCATTGAAGAATAATGGAACAAATGTAGAGGCAAGACTCGTGAATCAACACCTTTACAAGGCCCTTGAGGGGTCAGGTTTCCTTATTTTGGACATAACCCACCTGAGTGAGTTCAGAGCAGATGCCCATCCATCTGCAACTGGTGGGAAGAAACATGATGATTGTATGCACTGGTGCTTGCCAGGAATAACAGATACTTGGAATGACTTGCTCATAGCACAGTTAAATAATGTCCTGATTTAG

Protein Analysis

161

Amino Acids

18.14

Weight (kDa)

6.22

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 7 - 154 7.6e-37 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018513)

Species Orthologous Gene IDs
malus_domestica MD05G1110600.v1.1
rosa_chinensis RchiOBHm_Chr6g0247311
rosa_roxburghii Rroxscaffold_180G00433560
rosa_samantha Rh6AG039100 Rh6AG039300 Rh6AG040100 Rh6BG034600 Rh6CG034500 Rh6DG032200
rosa_wichuraiana Rw0G020480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 217
AcsI RAATTY 1 cut(s) 134
AfaI GTAC 2 cut(s) 54, 145
AfiI CCNNNNNNNGG 5 cut(s) 65, 76, 301, 312, 331
AgsI TTSAA 3 cut(s) 26, 167, 250
AjnI CCWGG 2 cut(s) 121, 424
AleI CACNNNNGTG 1 cut(s) 348
Alw26I GTCTC 1 cut(s) 106
AoxI GGCC 2 cut(s) 67, 303
ApoI RAATTY 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 304
AsuHPI GGTGA 2 cut(s) 144, 185
BauI CACGAG 1 cut(s) 281
BccI CCATC 2 cut(s) 378, 382
BciT130I CCWGG 2 cut(s) 123, 426
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 2 cut(s) 156, 221
BfuAI ACCTGC 1 cut(s) 217
Bme1390I CCNGG 2 cut(s) 123, 426
BmgT120I GGNCC 1 cut(s) 304
BmiI GGNNCC 2 cut(s) 45, 182
BmrFI CCNGG 2 cut(s) 123, 426
BmsI GCATC 1 cut(s) 355
BpmI CTGGAG 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 184
Bsc4I CCNNNNNNNGG 5 cut(s) 65, 76, 301, 312, 331
Bse118I RCCGGY 1 cut(s) 201
Bse1I ACTGG 4 cut(s) 50, 182, 388, 419
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 2 cut(s) 123, 426
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 2 cut(s) 55, 370
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 5 cut(s) 65, 76, 301, 312, 331
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 4 cut(s) 50, 182, 388, 419
BshFI GGCC 2 cut(s) 69, 305
BsiSI CCGG 1 cut(s) 202
BslI CCNNNNNNNGG 5 cut(s) 65, 76, 301, 312, 331
BsmAI GTCTC 1 cut(s) 106
BsnI GGCC 2 cut(s) 69, 305
BspANI GGCC 2 cut(s) 69, 305
BspCNI CTCAG 1 cut(s) 339
BspLI GGNNCC 2 cut(s) 45, 182
BspMI ACCTGC 1 cut(s) 217
BsrFI RCCGGY 1 cut(s) 201
BsrI ACTGG 4 cut(s) 50, 182, 388, 419
BssAI RCCGGY 1 cut(s) 201
BssECI CCNNGG 1 cut(s) 184
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 184
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 2 cut(s) 123, 426
Bst4CI ACNGT 1 cut(s) 465
Bst6I CTCTTC 2 cut(s) 104, 245
BstC8I GCNNGC 1 cut(s) 422
BstDEI CTNAG 1 cut(s) 347
BstENI CCTNNNNNAGG 1 cut(s) 299
BstF5I GGATG 2 cut(s) 55, 370
BstMAI GTCTC 1 cut(s) 106
BstNI CCWGG 2 cut(s) 123, 426
BstSCI CCNGG 2 cut(s) 121, 424
BsuRI GGCC 2 cut(s) 69, 305
BtsCI GGATG 2 cut(s) 55, 370
BtsIMutI CAGTG 1 cut(s) 412
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 1 cut(s) 422
Cfr10I RCCGGY 1 cut(s) 201
Cfr13I GGNCC 1 cut(s) 304
CsiI ACCWGGT 1 cut(s) 121
Csp6I GTAC 2 cut(s) 53, 144
CviAII CATG 2 cut(s) 91, 398
CviJI RGCY 3 cut(s) 37, 69, 305
CviKI_1 RGCY 3 cut(s) 37, 69, 305
CviQI GTAC 2 cut(s) 53, 144
DdeI CTNAG 1 cut(s) 347
Eam1104I CTCTTC 2 cut(s) 104, 245
EarI CTCTTC 2 cut(s) 104, 245
Eco130I CCWWGG 1 cut(s) 184
EcoNI CCTNNNNNAGG 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 304
EcoRII CCWGG 2 cut(s) 121, 424
EcoT14I CCWWGG 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 2 cut(s) 94, 401
FaiI YATR 7 cut(s) 77, 83, 92, 338, 399, 410, 458
FatI CATG 2 cut(s) 90, 397
FokI GGATG 2 cut(s) 42, 357
FspBI CTAG 2 cut(s) 156, 221
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 2 cut(s) 69, 305
HapII CCGG 1 cut(s) 202
Hin1II CATG 2 cut(s) 94, 401
HinfI GANTC 2 cut(s) 279, 286
HpaII CCGG 1 cut(s) 202
HphI GGTGA 2 cut(s) 144, 185
Hpy188I TCNGA 1 cut(s) 359
Hpy188III TCNNGA 3 cut(s) 59, 283, 478
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 1 cut(s) 465
HpyCH4V TGCA 3 cut(s) 128, 380, 412
HpyF3I CTNAG 1 cut(s) 347
Hsp92II CATG 2 cut(s) 94, 401
LweI GCATC 1 cut(s) 355
MabI ACCWGGT 1 cut(s) 121
MaeI CTAG 2 cut(s) 156, 221
MaeIII GTNAC 2 cut(s) 173, 197
MboII GAAGA 4 cut(s) 121, 232, 262, 403
MluCI AATT 1 cut(s) 134
MlyI GAGTC 1 cut(s) 273
MnlI CCTC 6 cut(s) 57, 80, 164, 226, 265, 304
MseI TTAA 1 cut(s) 467
MslI CAYNNNNRTG 3 cut(s) 60, 95, 348
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 2 cut(s) 123, 426
MvaI CCWGG 2 cut(s) 123, 426
NlaIII CATG 2 cut(s) 94, 401
NlaIV GGNNCC 2 cut(s) 45, 182
NmuCI GTSAC 1 cut(s) 173
OliI CACNNNNGTG 1 cut(s) 348
PfeI GAWTC 1 cut(s) 286
PleI GAGTC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 273
Psp6I CCWGG 2 cut(s) 121, 424
PspGI CCWGG 2 cut(s) 121, 424
PspN4I GGNNCC 2 cut(s) 45, 182
PspPI GGNCC 1 cut(s) 304
RsaI GTAC 2 cut(s) 54, 145
RsaNI GTAC 2 cut(s) 53, 144
RseI CAYNNNNRTG 3 cut(s) 60, 95, 348
SaqAI TTAA 1 cut(s) 467
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 2 cut(s) 123, 426
SetI ASST 8 cut(s) 124, 157, 175, 190, 231, 297, 322, 348
SexAI ACCWGGT 1 cut(s) 121
SfaNI GCATC 1 cut(s) 355
SmiMI CAYNNNNRTG 3 cut(s) 60, 95, 348
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 1 cut(s) 134
SspMI CTAG 2 cut(s) 156, 221
StyD4I CCNGG 2 cut(s) 121, 424
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 1 cut(s) 465
TaqI TCGA 1 cut(s) 72
TasI AATT 1 cut(s) 134
TatI WGTACW 1 cut(s) 52
TfiI GAWTC 1 cut(s) 286
Tru1I TTAA 1 cut(s) 467
Tru9I TTAA 1 cut(s) 467
TscAI CASTG 1 cut(s) 419
TseFI GTSAC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 2 cut(s) 17, 98
TspRI CASTG 1 cut(s) 419
XagI CCTNNNNNAGG 1 cut(s) 299
XapI RAATTY 1 cut(s) 134
XspI CTAG 2 cut(s) 156, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.