Rh6BG034600

Protein trichome birefringence-like 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0018513
Physical Location & Seq
Forward (+)
5395809 .. 5397569
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG034600.1

Sequence Viewer

Length: 804 bp
ATGTTTGTTTCGCTGTTCTGTTCGCTGAAACGCGTGTCGAGTGAAGTAAAGAAGTGGCGGCCAGTTGGTGCTGATCGCGGGTTCACATTTCTTCAGTACAATGTTACGCTTGCGTATCATCGGACAAATCTTTTGGCTCGTTACGGTAGGTGGTTGGCCAATTCTAATGGTGGCGTGCTTGGATATAAAGAGGGTTATAGAGTTGATGTTGACATTCCGGTTGACACATGGGCCGAATCTCTAAGCTTTCATGACATTTTGATATTCAACACAGGGCACTGGTATTGGGCTCCTGCAAAGTTTGACCCTATGAAGTCGCCGTTGCTTTTCTTTGAAAATGGTCAACCTGTGGTTCCTCCAGTACTTCCTGATGTTGGCCTTGATATGGTTATGAAACACATGGTAATGTTTGTGGAGAAGAGACTGAAGCCTGGTGCAATCAAATTCTTTCGTACTCAATCACCTAGACATTTTGAAGGAGGTGACTGGAACCAAGGTGGTTCTTGTAACCGGCAAAACCCTTTGTTGCCAGAGCAGGTTGAGGAACTCTTCTCATTGAAGAATAATGGAACAAATGTAGAGGCACGACTCGTGAATCAACACCTTTACAAGGCCCTTGAGGGGTCAGGTTTCCATATTTTGGACATAACCCACCTGAGTGAGTTCAGAGCGGATGCCCATCCATCTGCAGCTGGTGGGAAGAAGCACGATGATTGTATGCACTGGTGCTTGCCAGGAATAACAGATACTTGGAATGACTTGTTCATAGCACAGTTAAATAATGTCAAATTTAGAACAAAATGA

Protein Analysis

267

Amino Acids

30.5

Weight (kDa)

8.61

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 2 - 257 6.8e-54 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018513)

Species Orthologous Gene IDs
malus_domestica MD05G1110600.v1.1
rosa_chinensis RchiOBHm_Chr6g0247311
rosa_roxburghii Rroxscaffold_180G00433560
rosa_samantha Rh6AG039100 Rh6AG039300 Rh6AG040100 Rh6BG034600 Rh6CG034500 Rh6DG032200
rosa_wichuraiana Rw0G020480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 526
AccB7I CCANNNNNTGG 1 cut(s) 640
AccBSI CCGCTC 1 cut(s) 671
AccII CGCG 2 cut(s) 33, 78
AciI CCGC 3 cut(s) 58, 78, 671
AcoI YGGCCR 2 cut(s) 59, 156
AcsI RAATTY 2 cut(s) 443, 788
AcuI CTGAAG 2 cut(s) 77, 446
AfaI GTAC 3 cut(s) 98, 363, 454
AfiI CCNNNNNNNGG 5 cut(s) 374, 385, 610, 621, 640
AflIII ACRYGT 1 cut(s) 31
AgsI TTSAA 4 cut(s) 268, 335, 476, 559
AjnI CCWGG 2 cut(s) 430, 733
AleI CACNNNNGTG 1 cut(s) 657
AluBI AGCT 2 cut(s) 246, 692
AluI AGCT 2 cut(s) 246, 692
Alw26I GTCTC 1 cut(s) 415
AoxI GGCC 5 cut(s) 59, 156, 231, 376, 612
ApeKI GCWGC 1 cut(s) 689
ApoI RAATTY 2 cut(s) 443, 788
ArsI GACNNNNNNTTYG 2 cut(s) 115, 147
AspS9I GGNCC 2 cut(s) 231, 613
AsuHPI GGTGA 2 cut(s) 453, 494
BaeGI GKGCMC 1 cut(s) 279
BalI TGGCCA 1 cut(s) 158
BanII GRGCYC 1 cut(s) 292
BauI CACGAG 1 cut(s) 590
BbvI GCAGC 1 cut(s) 701
BccI CCATC 2 cut(s) 687, 691
BceAI ACGGC 1 cut(s) 304
BciT130I CCWGG 2 cut(s) 432, 735
BcoDI GTCTC 1 cut(s) 415
BfaI CTAG 1 cut(s) 465
BfmI CTRYAG 1 cut(s) 687
BfuAI ACCTGC 1 cut(s) 526
BisI GCNGC 2 cut(s) 59, 690
BlsI GCNGC 2 cut(s) 60, 691
BmcAI AGTACT 1 cut(s) 363
Bme1390I CCNGG 2 cut(s) 432, 735
BmgT120I GGNCC 2 cut(s) 231, 613
BmiI GGNNCC 3 cut(s) 291, 354, 491
BmrFI CCNGG 2 cut(s) 432, 735
BmsI GCATC 1 cut(s) 664
BpmI CTGGAG 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 638
BsaBI GATNNNNATC 1 cut(s) 678
BsaJI CCNNGG 1 cut(s) 493
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 5 cut(s) 374, 385, 610, 621, 640
Bse118I RCCGGY 1 cut(s) 510
Bse1I ACTGG 5 cut(s) 62, 284, 359, 491, 728
Bse8I GATNNNNATC 1 cut(s) 678
BseBI CCWGG 2 cut(s) 432, 735
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 2 cut(s) 679, 679
BseJI GATNNNNATC 1 cut(s) 678
BseLI CCNNNNNNNGG 5 cut(s) 374, 385, 610, 621, 640
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 5 cut(s) 62, 284, 359, 491, 728
BseSI GKGCMC 1 cut(s) 279
BseXI GCAGC 1 cut(s) 701
Bsh1236I CGCG 2 cut(s) 33, 78
BshFI GGCC 5 cut(s) 61, 158, 233, 378, 614
BsiSI CCGG 2 cut(s) 218, 511
BslI CCNNNNNNNGG 5 cut(s) 374, 385, 610, 621, 640
BsmAI GTCTC 1 cut(s) 415
BsnI GGCC 5 cut(s) 61, 158, 233, 378, 614
Bsp1286I GDGCHC 2 cut(s) 279, 292
Bsp143I GATC 1 cut(s) 73
BspACI CCGC 3 cut(s) 58, 78, 671
BspANI GGCC 5 cut(s) 61, 158, 233, 378, 614
BspCNI CTCAG 1 cut(s) 648
BspFNI CGCG 2 cut(s) 33, 78
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 3 cut(s) 291, 354, 491
BspMAI CTGCAG 1 cut(s) 691
BspMI ACCTGC 1 cut(s) 526
BsrBI CCGCTC 1 cut(s) 671
BsrFI RCCGGY 1 cut(s) 510
BsrI ACTGG 5 cut(s) 62, 284, 359, 491, 728
BssAI RCCGGY 1 cut(s) 510
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 1 cut(s) 73
BssSI CACGAG 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 493
Bst2BI CACGAG 1 cut(s) 590
Bst2UI CCWGG 2 cut(s) 432, 735
Bst4CI ACNGT 2 cut(s) 146, 774
Bst6I CTCTTC 2 cut(s) 413, 554
BstC8I GCNNGC 3 cut(s) 111, 176, 731
BstDEI CTNAG 2 cut(s) 242, 656
BstENI CCTNNNNNAGG 1 cut(s) 608
BstF5I GGATG 2 cut(s) 679, 679
BstFNI CGCG 2 cut(s) 33, 78
BstKTI GATC 1 cut(s) 76
BstMAI GTCTC 1 cut(s) 415
BstMBI GATC 1 cut(s) 73
BstNI CCWGG 2 cut(s) 432, 735
BstSCI CCNGG 2 cut(s) 430, 733
BstSFI CTRYAG 1 cut(s) 687
BstSLI GKGCMC 1 cut(s) 279
BstUI CGCG 2 cut(s) 33, 78
BstV1I GCAGC 1 cut(s) 701
BsuRI GGCC 5 cut(s) 61, 158, 233, 378, 614
BtsCI GGATG 2 cut(s) 679, 679
BtsIMutI CAGTG 2 cut(s) 277, 721
BveI ACCTGC 1 cut(s) 526
Cac8I GCNNGC 3 cut(s) 111, 176, 731
CciI TCATGA 1 cut(s) 250
Cfr10I RCCGGY 1 cut(s) 510
Cfr13I GGNCC 2 cut(s) 231, 613
Csp6I GTAC 3 cut(s) 97, 362, 453
CviAII CATG 3 cut(s) 228, 251, 400
CviQI GTAC 3 cut(s) 97, 362, 453
DdeI CTNAG 2 cut(s) 242, 656
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
EaeI YGGCCR 2 cut(s) 59, 156
Eam1104I CTCTTC 2 cut(s) 413, 554
EarI CTCTTC 2 cut(s) 413, 554
Eco130I CCWWGG 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 292
Eco57I CTGAAG 2 cut(s) 77, 446
EcoNI CCTNNNNNAGG 1 cut(s) 608
EcoO109I RGGNCCY 1 cut(s) 613
EcoRII CCWGG 2 cut(s) 430, 733
EcoT14I CCWWGG 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 493
FaeI CATG 3 cut(s) 231, 254, 403
FatI CATG 3 cut(s) 227, 250, 399
FauI CCCGC 1 cut(s) 71
Fnu4HI GCNGC 2 cut(s) 59, 690
FokI GGATG 2 cut(s) 666, 686
FriOI GRGCYC 1 cut(s) 292
Fsp4HI GCNGC 2 cut(s) 59, 690
FspBI CTAG 1 cut(s) 465
GluI GCNGC 2 cut(s) 59, 690
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 5 cut(s) 61, 158, 233, 378, 614
HapII CCGG 2 cut(s) 218, 511
Hin1II CATG 3 cut(s) 231, 254, 403
HincII GTYRAC 3 cut(s) 211, 223, 344
HindII GTYRAC 3 cut(s) 211, 223, 344
HindIII AAGCTT 1 cut(s) 244
HinfI GANTC 3 cut(s) 236, 588, 595
HpaII CCGG 2 cut(s) 218, 511
HphI GGTGA 2 cut(s) 453, 494
Hpy166II GTNNAC 4 cut(s) 84, 211, 223, 344
Hpy188I TCNGA 2 cut(s) 123, 668
Hpy188III TCNNGA 3 cut(s) 251, 368, 592
Hpy8I GTNNAC 4 cut(s) 84, 211, 223, 344
HpyAV CCTTC 1 cut(s) 470
HpyCH4III ACNGT 2 cut(s) 146, 774
HpyCH4V TGCA 4 cut(s) 296, 437, 689, 721
HpyF3I CTNAG 2 cut(s) 242, 656
Hsp92II CATG 3 cut(s) 231, 254, 403
Kzo9I GATC 1 cut(s) 73
LmnI GCTCC 1 cut(s) 295
Lsp1109I GCAGC 1 cut(s) 701
LweI GCATC 1 cut(s) 664
MaeI CTAG 1 cut(s) 465
MaeIII GTNAC 4 cut(s) 103, 140, 482, 506
MalI GATC 1 cut(s) 75
MbiI CCGCTC 1 cut(s) 671
MboI GATC 1 cut(s) 73
MboII GAAGA 5 cut(s) 83, 430, 541, 571, 712
MhlI GDGCHC 2 cut(s) 279, 292
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 3 cut(s) 160, 443, 788
MluI ACGCGT 1 cut(s) 31
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 1 cut(s) 582
MnlI CCTC 6 cut(s) 184, 366, 473, 535, 574, 613
Mox20I TGGCCA 1 cut(s) 158
MscI TGGCCA 1 cut(s) 158
MseI TTAA 1 cut(s) 776
MslI CAYNNNNRTG 2 cut(s) 404, 657
Msp20I TGGCCA 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 692
MspI CCGG 2 cut(s) 218, 511
MspR9I CCNGG 2 cut(s) 432, 735
MvaI CCWGG 2 cut(s) 432, 735
MvnI CGCG 2 cut(s) 33, 78
NdeII GATC 1 cut(s) 73
NlaIII CATG 3 cut(s) 231, 254, 403
NlaIV GGNNCC 3 cut(s) 291, 354, 491
NmuCI GTSAC 1 cut(s) 482
OliI CACNNNNGTG 1 cut(s) 657
PagI TCATGA 1 cut(s) 250
PfeI GAWTC 2 cut(s) 236, 595
PflMI CCANNNNNTGG 1 cut(s) 640
PkrI GCNGC 2 cut(s) 60, 691
PleI GAGTC 1 cut(s) 582
PpsI GAGTC 1 cut(s) 582
Psp6I CCWGG 2 cut(s) 430, 733
PspGI CCWGG 2 cut(s) 430, 733
PspN4I GGNNCC 3 cut(s) 291, 354, 491
PspPI GGNCC 2 cut(s) 231, 613
PstI CTGCAG 1 cut(s) 691
PvuII CAGCTG 1 cut(s) 692
RsaI GTAC 3 cut(s) 98, 363, 454
RsaNI GTAC 3 cut(s) 97, 362, 453
RseI CAYNNNNRTG 2 cut(s) 404, 657
SaqAI TTAA 1 cut(s) 776
SatI GCNGC 2 cut(s) 59, 690
Sau3AI GATC 1 cut(s) 73
Sau96I GGNCC 2 cut(s) 231, 613
ScaI AGTACT 1 cut(s) 363
SchI GAGTC 1 cut(s) 582
ScrFI CCNGG 2 cut(s) 432, 735
SduI GDGCHC 2 cut(s) 279, 292
SfaNI GCATC 1 cut(s) 664
SfcI CTRYAG 1 cut(s) 687
SmiMI CAYNNNNRTG 2 cut(s) 404, 657
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
Sse9I AATT 3 cut(s) 160, 443, 788
SsiI CCGC 3 cut(s) 58, 78, 671
SspMI CTAG 1 cut(s) 465
StyD4I CCNGG 2 cut(s) 430, 733
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 2 cut(s) 146, 774
TaqI TCGA 1 cut(s) 38
TasI AATT 3 cut(s) 160, 443, 788
TatI WGTACW 2 cut(s) 96, 361
TauI GCSGC 1 cut(s) 61
TfiI GAWTC 2 cut(s) 236, 595
Tru1I TTAA 1 cut(s) 776
Tru9I TTAA 1 cut(s) 776
TscAI CASTG 2 cut(s) 284, 728
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 1 cut(s) 689
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 4 cut(s) 239, 326, 407, 754
TspRI CASTG 2 cut(s) 284, 728
Van91I CCANNNNNTGG 1 cut(s) 640
XagI CCTNNNNNAGG 1 cut(s) 608
XapI RAATTY 2 cut(s) 443, 788
XspI CTAG 1 cut(s) 465
ZrmI AGTACT 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.