MD05G1165800.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008443
Physical Location & Seq
Forward (+)
29492763 .. 29496593
3831 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1165800.v1.1.491

Sequence Viewer

Length: 1707 bp
ATGGAGAATGATTTTTTCCTAAATGCCGGAATCCCACCTCCGCTTCACTTTGAACAAGCATCCTCAATGCCGGCATGGCGGTCTTCTTTCTCGACGGCCATAGACATCCAGTCCTCTACCGCGGACCGGAATTGCTCTTTGGAGCAGTCCCCAGACTGCTTCTACAATCCCAACTGGGTCAACAAGTCAACCAACCAGAACATCCACTTCGAATCAGCCCTGAGTCCAATGGTCTCATCTCCGGCGGCGTCCAATTCCAATATCTCGAACGAGAGCTTCATGATCAGAGAACTGATTGGAAAGCTCGGAAACATCAGAGGCACCGGCGAAATCTCGCCGCATTCCGAGTCGTTGCTGGGAATTCAAAATTCTTACATGGGTAGAAATGGAAATGCAAGTGCCAACACTTCAAGCTACAGCACTCCGTTGAACTCGCCGCCCAAACTAAGGCTCCCCGTTGCGGATCACCATTTGAAGAAAGAAAACCTACCTAGTTTGGGAAACTCCATGGCCTTGAAATCCAGCGTGGCCGAGTTCTCAGCCGACCCTGGATTCGCCGAGAGGGCAGCAAAGTTTTCGTGTTTCGGGAGCAGGAGCTTCAATGGTAGAACGCCACAGCTAGGAATGAACAATAACAACAGTACTGAACAACCGCCGTTTCGATCCCATCCCGCCGCGAAAAATGGCGGCAAGCTTCCTCGTGTTTCGAGCAGTCCGTCGCTGAAGGCACTCGGATCTCAAACGAGTATGCAGGAAAAGATGAGTGCTCTGCTGCAGGATAGGAGTGAGTTCCCCATTTCCCGAGAGGAATCGACGATTTCTGAGCAGAATCCAAATGGGGAGACCAATTCCATGGATTTGAATTCTAGGAAAAGAAAATCAGTTTCCAAGGGAAAAGCAAAGGATCCTCCTCCTCCAATATCCCCATCTCCAACTTCCACAAAGGGGACTGAAGCTGATGAAAATTCGAATGCAAAGAGATGCAAGCCAAGTGAAAACAATGGGAATGACCAAAATGGCTCTGTGAAAGCAGAGGAGGATGCAAAGGGAAGCACTAGTTCTGATGAGAAGCAAACCAAGACTGGTCCAAAGCCACCTGAGCCTCCCAAGGATTATATTCATGTAAGAGCAAGAAGGGGTCAAGCAACTGATAGCCATAGCCTTGCTGAAAGGGTAAGAAGAGAAAAAATTAGTGAAAGAATGAAGCTTCTTCAAGATCTTGTGCCGAATTGCAACAAGGTGACTGGAAAAGCACTTATGCTTGATGAGATTATTAATTATGTGCAGTCATTACAACGCCAAGTCGAGTTTCTCTCCATGAAGTTGTCTTCTGTGAACACCAGGCTGGATTTCAACATGGAAACCCTAATGTCAAAAGAGATATTTCAGCAAAACGGTGGTTTGCCACAGCATCCAATATTCCCATCAGATTCCTCGGCACAAGCCATTTATGGACACCAACGCCAGCAAAATCCAGTACTTTCTAATGGGGCTGTGGACCCCTTGGATAATACCTCACTATACCAAAGCCTTGGGATGCAATTACCTCCTCTCAGTGGTTTTAGTAGTGAACGCATTCCTCAGTTTGCAGCATTTGGTGAAGATGATCTACAAACCATTGTTCAGATGGGTTTTGGCCAAAATCCAACCAGGGAATCAGAATTGCATGGTTCAAATCAAGTATCCCACATGAAAATTGAGCTCTGA

Protein Analysis

569

Amino Acids

62.13

Weight (kDa)

7.2

Isoelectric Point (pI)

54.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 385 - 432 6.3e-07 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 320
AccII CGCG 2 cut(s) 122, 677
AclWI GGATC 5 cut(s) 471, 657, 742, 899, 912
AcoI YGGCCR 3 cut(s) 96, 528, 1636
AcsI RAATTY 4 cut(s) 360, 367, 862, 964
AcuI CTGAAG 2 cut(s) 743, 972
AcyI GRCGYC 1 cut(s) 248
AfaI GTAC 2 cut(s) 643, 1479
AfiI CCNNNNNNNGG 7 cut(s) 126, 447, 460, 497, 620, 945, 1556
AhdI GACNNNNNGTC 1 cut(s) 109
AhlI ACTAGT 1 cut(s) 1055
AjnI CCWGG 3 cut(s) 547, 1340, 1649
AjuI GAANNNNNNNTTGG 4 cut(s) 122, 154, 1293, 1325
AluBI AGCT 9 cut(s) 276, 304, 414, 597, 619, 694, 956, 1207, 1702
AluI AGCT 9 cut(s) 276, 304, 414, 597, 619, 694, 956, 1207, 1702
Alw21I GWGCWC 2 cut(s) 769, 1704
Alw26I GTCTC 2 cut(s) 238, 836
AlwI GGATC 5 cut(s) 471, 657, 742, 899, 912
Ama87I CYCGRG 1 cut(s) 801
AoxI GGCC 4 cut(s) 96, 510, 528, 1636
ApeKI GCWGC 3 cut(s) 566, 772, 1589
ApoI RAATTY 4 cut(s) 360, 367, 862, 964
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AseI ATTAAT 1 cut(s) 1275
Asp700I GAANNNNTTC 1 cut(s) 1575
AspS9I GGNCC 3 cut(s) 124, 1085, 1498
AsuHPI GGTGA 3 cut(s) 458, 1252, 1610
AsuII TTCGAA 2 cut(s) 210, 968
AvaI CYCGRG 1 cut(s) 801
AvaII GGWCC 3 cut(s) 124, 1085, 1498
BalI TGGCCA 1 cut(s) 1638
BamHI GGATCC 1 cut(s) 904
BanI GGYRCC 1 cut(s) 320
BanII GRGCYC 1 cut(s) 1704
BarI GAAGNNNNNNTAC 2 cut(s) 1593, 1625
BauI CACGAG 1 cut(s) 699
BbsI GAAGAC 2 cut(s) 75, 1320
Bbv12I GWGCWC 2 cut(s) 769, 1704
BbvI GCAGC 3 cut(s) 578, 759, 1601
BccI CCATC 4 cut(s) 675, 934, 1432, 1621
BceAI ACGGC 2 cut(s) 111, 640
BcgI CGANNNNNNTGC 2 cut(s) 558, 592
BciT130I CCWGG 3 cut(s) 549, 1342, 1651
BciVI GTATCC 1 cut(s) 1693
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 238, 836
BcuI ACTAGT 1 cut(s) 1055
BfaI CTAG 4 cut(s) 492, 620, 867, 1056
BfmI CTRYAG 2 cut(s) 415, 773
BfuI GTATCC 1 cut(s) 1693
BglI GCCNNNNNGGC 2 cut(s) 76, 563
BglII AGATCT 1 cut(s) 1216
BisI GCNGC 8 cut(s) 246, 338, 437, 567, 675, 688, 773, 1590
BlsI GCNGC 8 cut(s) 247, 339, 438, 568, 676, 689, 774, 1591
BmcAI AGTACT 2 cut(s) 643, 1479
Bme1390I CCNGG 3 cut(s) 549, 1342, 1651
Bme18I GGWCC 3 cut(s) 124, 1085, 1498
BmeRI GACNNNNNGTC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 801
BmgT120I GGNCC 3 cut(s) 124, 1085, 1498
BmiI GGNNCC 4 cut(s) 322, 452, 906, 1500
BmrFI CCNGG 3 cut(s) 549, 1342, 1651
BmrI ACTGGG 1 cut(s) 184
BmsI GCATC 5 cut(s) 68, 971, 1030, 1420, 1527
BmuI ACTGGG 1 cut(s) 184
BpiI GAAGAC 2 cut(s) 75, 1320
BplI GAGNNNNNCTC 2 cut(s) 1298, 1330
Bpu10I CCTNAGC 1 cut(s) 1098
Bpu14I TTCGAA 2 cut(s) 210, 968
BsaHI GRCGYC 1 cut(s) 248
BsaI GGTCTC 2 cut(s) 238, 836
BsaWI WCCGGW 1 cut(s) 126
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bsc4I CCNNNNNNNGG 7 cut(s) 126, 447, 460, 497, 620, 945, 1556
Bse118I RCCGGY 2 cut(s) 70, 323
Bse1I ACTGG 5 cut(s) 109, 179, 1087, 1249, 1475
BseBI CCWGG 3 cut(s) 549, 1342, 1651
BseGI GGATG 7 cut(s) 59, 105, 201, 667, 1045, 1411, 1542
BseLI CCNNNNNNNGG 7 cut(s) 126, 447, 460, 497, 620, 945, 1556
BseMII CTCAG 6 cut(s) 212, 552, 813, 1089, 1567, 1595
BseNI ACTGG 5 cut(s) 109, 179, 1087, 1249, 1475
BseRI GAGGAG 4 cut(s) 900, 903, 1049, 1539
BseXI GCAGC 3 cut(s) 578, 759, 1601
BseYI CCCAGC 1 cut(s) 355
BsgI GTGCAG 1 cut(s) 1304
Bsh1236I CGCG 2 cut(s) 122, 677
BshFI GGCC 4 cut(s) 98, 512, 530, 1638
BshNI GGYRCC 1 cut(s) 320
BsiHKAI GWGCWC 2 cut(s) 769, 1704
BsiHKCI CYCGRG 1 cut(s) 801
BsiSI CCGG 5 cut(s) 27, 71, 127, 242, 324
BslFI GGGAC 2 cut(s) 133, 961
BslI CCNNNNNNNGG 7 cut(s) 126, 447, 460, 497, 620, 945, 1556
BsmAI GTCTC 2 cut(s) 238, 836
BsmFI GGGAC 2 cut(s) 133, 961
BsmI GAATGC 3 cut(s) 340, 976, 1575
BsnI GGCC 4 cut(s) 98, 512, 530, 1638
Bso31I GGTCTC 2 cut(s) 238, 836
BsoBI CYCGRG 1 cut(s) 801
Bsp119I TTCGAA 2 cut(s) 210, 968
Bsp1286I GDGCHC 2 cut(s) 769, 1704
Bsp143I GATC 7 cut(s) 282, 463, 662, 734, 904, 1216, 1606
Bsp19I CCATGG 2 cut(s) 507, 852
BspANI GGCC 4 cut(s) 98, 512, 530, 1638
BspCNI CTCAG 6 cut(s) 213, 551, 814, 1090, 1566, 1594
BspFNI CGCG 2 cut(s) 122, 677
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 4 cut(s) 322, 452, 906, 1500
BspMAI CTGCAG 1 cut(s) 777
BspPI GGATC 5 cut(s) 471, 657, 742, 899, 912
BspT104I TTCGAA 2 cut(s) 210, 968
BspT107I GGYRCC 1 cut(s) 320
BspTNI GGTCTC 2 cut(s) 238, 836
BsrFI RCCGGY 2 cut(s) 70, 323
BsrI ACTGG 5 cut(s) 109, 179, 1087, 1249, 1475
BssAI RCCGGY 2 cut(s) 70, 323
BssMI GATC 7 cut(s) 282, 463, 662, 734, 904, 1216, 1606
BssNI GRCGYC 1 cut(s) 248
BssSI CACGAG 1 cut(s) 699
BssT1I CCWWGG 6 cut(s) 507, 852, 888, 1107, 1503, 1531
Bst2BI CACGAG 1 cut(s) 699
Bst2UI CCWGG 3 cut(s) 549, 1342, 1651
Bst4CI ACNGT 2 cut(s) 641, 1397
Bst6I CTCTTC 1 cut(s) 1174
BstACI GRCGYC 1 cut(s) 248
BstBI TTCGAA 2 cut(s) 210, 968
BstC8I GCNNGC 4 cut(s) 72, 692, 986, 1466
BstDEI CTNAG 7 cut(s) 221, 446, 538, 822, 1098, 1553, 1581
BstDSI CCRYGG 3 cut(s) 120, 507, 852
BstF5I GGATG 7 cut(s) 59, 105, 201, 667, 1045, 1411, 1542
BstFNI CGCG 2 cut(s) 122, 677
BstKTI GATC 7 cut(s) 285, 466, 665, 737, 907, 1219, 1609
BstMAI GTCTC 2 cut(s) 238, 836
BstMBI GATC 7 cut(s) 282, 463, 662, 734, 904, 1216, 1606
BstMWI GCNNNNNNNGC 3 cut(s) 76, 563, 1099
BstNI CCWGG 3 cut(s) 549, 1342, 1651
BstSCI CCNGG 3 cut(s) 547, 1340, 1649
BstSFI CTRYAG 2 cut(s) 415, 773
BstUI CGCG 2 cut(s) 122, 677
BstV1I GCAGC 3 cut(s) 578, 759, 1601
BstV2I GAAGAC 2 cut(s) 75, 1320
BstX2I RGATCY 3 cut(s) 734, 904, 1216
BstXI CCANNNNNNTGG 2 cut(s) 853, 1532
BstYI RGATCY 3 cut(s) 734, 904, 1216
BsuI GTATCC 1 cut(s) 1693
BsuRI GGCC 4 cut(s) 98, 512, 530, 1638
BtgI CCRYGG 3 cut(s) 120, 507, 852
BtsCI GGATG 7 cut(s) 59, 105, 201, 667, 1045, 1411, 1542
BtsIMutI CAGTG 1 cut(s) 1561
Cac8I GCNNGC 4 cut(s) 72, 692, 986, 1466
CciI TCATGA 1 cut(s) 279
Cfr10I RCCGGY 2 cut(s) 70, 323
Cfr13I GGNCC 3 cut(s) 124, 1085, 1498
Cfr42I CCGCGG 1 cut(s) 123
CpoI CGGWCCG 1 cut(s) 124
CseI GACGC 1 cut(s) 237
Csp6I GTAC 2 cut(s) 642, 1478
CspI CGGWCCG 1 cut(s) 124
CviQI GTAC 2 cut(s) 642, 1478
DdeI CTNAG 7 cut(s) 221, 446, 538, 822, 1098, 1553, 1581
DpnI GATC 7 cut(s) 284, 465, 664, 736, 906, 1218, 1608
DpnII GATC 7 cut(s) 282, 463, 662, 734, 904, 1216, 1606
DriI GACNNNNNGTC 1 cut(s) 109
EaeI YGGCCR 3 cut(s) 96, 528, 1636
Eam1104I CTCTTC 1 cut(s) 1174
Eam1105I GACNNNNNGTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 1174
Ecl136II GAGCTC 1 cut(s) 1702
Eco130I CCWWGG 6 cut(s) 507, 852, 888, 1107, 1503, 1531
Eco24I GRGCYC 1 cut(s) 1704
Eco31I GGTCTC 2 cut(s) 238, 836
Eco47I GGWCC 3 cut(s) 124, 1085, 1498
Eco53kI GAGCTC 1 cut(s) 1702
Eco57I CTGAAG 2 cut(s) 743, 972
Eco88I CYCGRG 1 cut(s) 801
EcoICRI GAGCTC 1 cut(s) 1702
EcoRI GAATTC 2 cut(s) 360, 862
EcoRII CCWGG 3 cut(s) 547, 1340, 1649
EcoT14I CCWWGG 6 cut(s) 507, 852, 888, 1107, 1503, 1531
EcoT38I GRGCYC 1 cut(s) 1704
ErhI CCWWGG 6 cut(s) 507, 852, 888, 1107, 1503, 1531
FaqI GGGAC 2 cut(s) 133, 961
FauI CCCGC 1 cut(s) 679
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 8 cut(s) 246, 338, 437, 567, 675, 688, 773, 1590
FokI GGATG 7 cut(s) 46, 92, 188, 654, 1052, 1398, 1549
FriOI GRGCYC 1 cut(s) 1704
Fsp4HI GCNGC 8 cut(s) 246, 338, 437, 567, 675, 688, 773, 1590
FspBI CTAG 4 cut(s) 492, 620, 867, 1056
GluI GCNGC 8 cut(s) 246, 338, 437, 567, 675, 688, 773, 1590
GsaI CCCAGC 1 cut(s) 359
HaeIII GGCC 4 cut(s) 98, 512, 530, 1638
HapII CCGG 5 cut(s) 27, 71, 127, 242, 324
HgaI GACGC 1 cut(s) 237
Hin1I GRCGYC 1 cut(s) 248
HincII GTYRAC 2 cut(s) 181, 189
HindII GTYRAC 2 cut(s) 181, 189
HindIII AAGCTT 2 cut(s) 692, 1205
HinfI GANTC 9 cut(s) 30, 212, 223, 347, 552, 809, 829, 1430, 1655
HpaII CCGG 5 cut(s) 27, 71, 127, 242, 324
HphI GGTGA 3 cut(s) 458, 1252, 1610
Hpy166II GTNNAC 5 cut(s) 181, 189, 1336, 1498, 1571
Hpy188III TCNNGA 6 cut(s) 91, 265, 280, 586, 801, 1214
Hpy8I GTNNAC 5 cut(s) 181, 189, 1336, 1498, 1571
Hpy99I CGWCG 3 cut(s) 97, 721, 817
HpyAV CCTTC 2 cut(s) 718, 1128
HpyCH4III ACNGT 2 cut(s) 641, 1397
HpyF10VI GCNNNNNNNGC 3 cut(s) 76, 563, 1099
HpyF3I CTNAG 7 cut(s) 221, 446, 538, 822, 1098, 1553, 1581
Hsp92I GRCGYC 1 cut(s) 248
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
Ksp22I TGATCA 1 cut(s) 282
KspI CCGCGG 1 cut(s) 123
Kzo9I GATC 7 cut(s) 282, 463, 662, 734, 904, 1216, 1606
LmnI GCTCC 4 cut(s) 142, 456, 588, 594
Lsp1109I GCAGC 3 cut(s) 578, 759, 1601
LweI GCATC 5 cut(s) 68, 971, 1030, 1420, 1527
MaeI CTAG 4 cut(s) 492, 620, 867, 1056
MaeIII GTNAC 1 cut(s) 1240
MalI GATC 7 cut(s) 284, 465, 664, 736, 906, 1218, 1608
MboI GATC 7 cut(s) 282, 463, 662, 734, 904, 1216, 1606
MboII GAAGA 6 cut(s) 75, 487, 1191, 1202, 1320, 1613
MflI RGATCY 3 cut(s) 734, 904, 1216
MhlI GDGCHC 2 cut(s) 769, 1704
MlsI TGGCCA 1 cut(s) 1638
MluNI TGGCCA 1 cut(s) 1638
MlyI GAGTC 2 cut(s) 232, 356
MmeI TCCRAC 2 cut(s) 956, 1670
Mox20I TGGCCA 1 cut(s) 1638
MroNI GCCGGC 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 1575
MscI TGGCCA 1 cut(s) 1638
MseI TTAA 1 cut(s) 1275
Msp20I TGGCCA 1 cut(s) 1638
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 5 cut(s) 27, 71, 127, 242, 324
MspR9I CCNGG 3 cut(s) 549, 1342, 1651
Mva1269I GAATGC 3 cut(s) 340, 976, 1575
MvaI CCWGG 3 cut(s) 549, 1342, 1651
MvnI CGCG 2 cut(s) 122, 677
MwoI GCNNNNNNNGC 3 cut(s) 76, 563, 1099
NaeI GCCGGC 1 cut(s) 72
NcoI CCATGG 2 cut(s) 507, 852
NdeII GATC 7 cut(s) 282, 463, 662, 734, 904, 1216, 1606
NgoMIV GCCGGC 1 cut(s) 70
NlaIV GGNNCC 4 cut(s) 322, 452, 906, 1500
NmeAIII GCCGAG 3 cut(s) 556, 583, 1415
NmuCI GTSAC 1 cut(s) 1240
NspV TTCGAA 2 cut(s) 210, 968
PagI TCATGA 1 cut(s) 279
PcsI WCGNNNNNNNCGW 1 cut(s) 713
PctI GAATGC 3 cut(s) 340, 976, 1575
PdiI GCCGGC 1 cut(s) 72
PdmI GAANNNNTTC 1 cut(s) 1575
PfeI GAWTC 7 cut(s) 30, 212, 552, 809, 829, 1430, 1655
PkrI GCNGC 8 cut(s) 247, 339, 438, 568, 676, 689, 774, 1591
PleI GAGTC 2 cut(s) 231, 355
PpsI GAGTC 2 cut(s) 231, 355
PshBI ATTAAT 1 cut(s) 1275
Psp124BI GAGCTC 1 cut(s) 1704
Psp6I CCWGG 3 cut(s) 547, 1340, 1649
PspFI CCCAGC 1 cut(s) 355
PspGI CCWGG 3 cut(s) 547, 1340, 1649
PspN4I GGNNCC 4 cut(s) 322, 452, 906, 1500
PspPI GGNCC 3 cut(s) 124, 1085, 1498
PstI CTGCAG 1 cut(s) 777
PsuI RGATCY 3 cut(s) 734, 904, 1216
RsaI GTAC 2 cut(s) 643, 1479
RsaNI GTAC 2 cut(s) 642, 1478
Rsr2I CGGWCCG 1 cut(s) 124
RsrII CGGWCCG 1 cut(s) 124
SacI GAGCTC 1 cut(s) 1704
SacII CCGCGG 1 cut(s) 123
SaqAI TTAA 1 cut(s) 1275
SatI GCNGC 8 cut(s) 246, 338, 437, 567, 675, 688, 773, 1590
Sau3AI GATC 7 cut(s) 282, 463, 662, 734, 904, 1216, 1606
Sau96I GGNCC 3 cut(s) 124, 1085, 1498
ScaI AGTACT 2 cut(s) 643, 1479
SchI GAGTC 2 cut(s) 232, 356
ScrFI CCNGG 3 cut(s) 549, 1342, 1651
SduI GDGCHC 2 cut(s) 769, 1704
SfaNI GCATC 5 cut(s) 68, 971, 1030, 1420, 1527
SfcI CTRYAG 2 cut(s) 415, 773
Sfr303I CCGCGG 1 cut(s) 123
SfuI TTCGAA 2 cut(s) 210, 968
SgrAI CRCCGGYG 1 cut(s) 323
SgrBI CCGCGG 1 cut(s) 123
SinI GGWCC 3 cut(s) 124, 1085, 1498
SpeI ACTAGT 1 cut(s) 1055
SspI AATATT 1 cut(s) 1419
SspMI CTAG 4 cut(s) 492, 620, 867, 1056
SstI GAGCTC 1 cut(s) 1704
StyD4I CCNGG 3 cut(s) 547, 1340, 1649
StyI CCWWGG 6 cut(s) 507, 852, 888, 1107, 1503, 1531
TaaI ACNGT 2 cut(s) 641, 1397
TaqI TCGA 8 cut(s) 92, 210, 266, 661, 707, 812, 968, 1305
TatI WGTACW 2 cut(s) 641, 1477
TauI GCSGC 5 cut(s) 248, 340, 439, 677, 690
TfiI GAWTC 7 cut(s) 30, 212, 552, 809, 829, 1430, 1655
Tru1I TTAA 1 cut(s) 1275
Tru9I TTAA 1 cut(s) 1275
TscAI CASTG 1 cut(s) 1561
TseFI GTSAC 1 cut(s) 1240
TseI GCWGC 3 cut(s) 566, 772, 1589
Tsp45I GTSAC 1 cut(s) 1240
TspDTI ATGAA 7 cut(s) 268, 641, 975, 1109, 1217, 1334, 1706
TspGWI ACGGA 2 cut(s) 414, 705
TspRI CASTG 1 cut(s) 1561
VpaK11BI GGWCC 3 cut(s) 124, 1085, 1498
VspI ATTAAT 1 cut(s) 1275
XapI RAATTY 4 cut(s) 360, 367, 862, 964
XcmI CCANNNNNNNNNTGG 1 cut(s) 1624
XmnI GAANNNNTTC 1 cut(s) 1575
XspI CTAG 4 cut(s) 492, 620, 867, 1056
ZrmI AGTACT 2 cut(s) 643, 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.