Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009912

Physical Location & Seq

Genomic Coordinates Forward (+)

29646084 .. 29648745

Gene Structure 2662 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1166600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1575 bp

ATGGCAGCAGCAGCAGCTCAGTGCTCCAGTTCCTTCATTTTATATTTTTATATTTTTATATCTGATTTTTCCTCAAATATCAAATCTTTGAGCTCCAAAAGTCACACATTCAGAATGTTTCTCTCTTTTTATTCTGGTAGCTCAAAACCTACAAAAATGGAGCTGGGTCTTCTTCCCGTGCTCATCCTCTGCCTCTCCGCTTCCATTTCCAGTGCGGAGCTCGTCGCCAAGGTGGGTGTGAACTACGGCCAGCTGGGCAACAACCTCCCCCCTCCGTCGCAGTCGGTGAAGCTCATCCAATCCCTCAATGCGAAGCGCGTCAAGCTCTACGACGCCAACCCCAAAATCCTCGCCGCCCTTCGCAACACTGACCTCCAGGTCTCCATCATGGTCCCCAACGAGCTCATCAGCAACATCTCCTCCAATCAAACTCTAGCCGATCAATGGGTCCGCACCAACGTCGTACCCTTCTACCCGGACACCCTGATTCGGTACCTCCTCGTGGGAAACGAAATCCTCAGCCAACCCAATAAGCAAATCTGGTTCAATCTCGTACCCGCAATGCGGAAAATCAAAGCTGCTCTAAAGACCCACCGGATTACAAAGGTGAAAGTCGGAACCCCATTGGCCATGGACGTCCTCGAATCGTCGTTTCCGCCTTCGAACGGCACGTTTCGGTCTGATATTTCGGGTTCGGTCATCAAACCCATGTTGAAATTCATCAACAAAACCAAGTCTTTCTTCTTCGTCGACTTGTACACGTATTTCCCCTGGTCCTCCGACCCGACCCACATCGATTTGGGCTACGCTCTCTTAGAATCCAAGAACATTACGGTCACCGACCCGGCTTCCGGCTTAACCTACCACAATCTCTTCGACCAAATGGTGGACGCTTTGATTTTCGCAATGAAAAGACTCGGGTACCCGGATACCCGCATTTGGATAGCCGAAACTGGTTGGCCCAGCGGCGGCGACTATGACCAGATTGGCGCTAACATCCGCAACGCCGCCACTTACAACCGAAACGTAGTAAAAAAATTCAACGCCAAACCACCGGTGGGGACGCCGGCCCGACCTGGTGCAGTGCTCCCGAGCTTCGTCTTTGCTCTATTCAACGAGAACACCAAACCGGGTCCCAGCACGGAGCGGAATTTCGGGTTTCTATACCCAAACGGGTCGAATGTGTACCCTATTGACTTAAGCGGGAAGACGCCCGAGTCGGAGTACGCGCCGTTGCCGGTGGCGACGAATAACGAGCCGTATAAGGGGCCGATTTGGTGCATGGTGGCGAAGGGAGCCAATCAGAGCGCGGTGGCGTCGGCGCTATCGTACGCCTGCTCGCAGGGGAATAAAACTTGTGATCCGATTCAACCCGGAGGAAAGTGCTTTAAACCGGATTCGTTGGCTTGGCACGCGAGCTATGCGTTTAGCGCGTATTGGTCTCAGTTTAGGAAGGCTGGTGGGAGTTGTTACTTTGGCGGGCTCGCTACTCAAACCATCAAGGATCCAAGTTACGGATCCTGCAAGTTCCCCGGTGCCAAACTATTAGCGACGAAGGATACAATGGCACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001871 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010208 GO:0010584 GO:0010927 GO:0016020 GO:0016043 GO:0016787 GO:0016798 GO:0022414 GO:0022607 GO:0030198 GO:0030246 GO:0030247 GO:0031224 GO:0031225 GO:0031226 GO:0032501 GO:0032502 GO:0032989 GO:0043062 GO:0044085 GO:0044425 GO:0044459 GO:0044464 GO:0045229 GO:0046658 GO:0048229 GO:0048316 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0061458 GO:0071840 GO:0071944 GO:0085029

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_17 X8

Protein Analysis

525

Amino Acids

57.37

Weight (kDa)

9.38

Isoelectric Point (pI)

43.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_17	PF00332	78 - 399	2.5e-97	Glycosyl hydrolases family 17
X8	PF07983	426 - 497	1.8e-18	X8 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009912)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07320
fragaria_vesca	FvH4_2g18182 FvH4_2g18200
malus_domestica	MD05G1166600.v1.1 MD10G1156600.v1.1
prunus_persica	Prupe.8G194400_v2.0.a1
pyrus_communis	pycom05g15540 pycom10g13480
rosa_chinensis	RchiOBHm_Chr6g0283561 RchiOBHm_Chr6g0283571
rosa_laevigata	RLG00000012816
rosa_multiflora	Rmu_sc0005719.1_g000009 Rmu_sc0007194.1_g000001
rosa_roxburghii	Rroxscaffold_7G00185370 Rroxscaffold_7G00185400
rosa_rugosa	Rorug06G0155800
rosa_samantha	Rh6AG267700 Rh6BG270200 Rh6CG270000 Rh6DG263600
rosa_wichuraiana	Rw6G023180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	377, 1216
AatII	GACGTC	1 cut(s)	639
Acc65I	GGTACC	2 cut(s)	492, 921
AccB1I	GGYRCC	4 cut(s)	492, 921, 1535, 1567
AccB7I	CCANNNNNTGG	1 cut(s)	886
AccBSI	CCGCTC	1 cut(s)	1147
AccI	GTMKAC	1 cut(s)	750
AccII	CGCG	5 cut(s)	318, 1229, 1310, 1415, 1433
AclWI	GGATC	5 cut(s)	1355, 1499, 1512, 1512, 1525
AcoI	YGGCCR	2 cut(s)	247, 627
AcsI	RAATTY	3 cut(s)	716, 1037, 1150
AcyI	GRCGYC	5 cut(s)	333, 636, 1064, 1211, 1316
AfaI	GTAC	8 cut(s)	465, 494, 555, 758, 923, 1187, 1226, 1331
AflII	CTTAAG	1 cut(s)	1198
AflIII	ACRYGT	1 cut(s)	759
AgeI	ACCGGT	1 cut(s)	1054
AgsI	TTSAA	5 cut(s)	547, 715, 1042, 1114, 1370
AjnI	CCWGG	3 cut(s)	375, 770, 1075
AjuI	GAANNNNNNNTTGG	2 cut(s)	725, 757
Alw21I	GWGCWC	6 cut(s)	26, 95, 183, 222, 405, 1089
Alw26I	GTCTC	2 cut(s)	385, 1446
AlwI	GGATC	5 cut(s)	1355, 1499, 1512, 1512, 1525
Ama87I	CYCGRG	3 cut(s)	917, 1090, 1214
AoxI	GGCC	5 cut(s)	247, 627, 959, 1068, 1268
ApeKI	GCWGC	5 cut(s)	5, 8, 11, 14, 578
ApoI	RAATTY	3 cut(s)	716, 1037, 1150
AsiGI	ACCGGT	1 cut(s)	1054
Asp718I	GGTACC	2 cut(s)	492, 921
AspLEI	GCGC	6 cut(s)	318, 992, 1231, 1310, 1324, 1433
AspS9I	GGNCC	7 cut(s)	391, 448, 774, 960, 1069, 1133, 1268
AsuC2I	CCSGG	6 cut(s)	476, 845, 926, 1131, 1374, 1533
AsuHPI	GGTGA	3 cut(s)	298, 619, 829
AsuII	TTCGAA	1 cut(s)	662
AvaI	CYCGRG	3 cut(s)	917, 1090, 1214
AvaII	GGWCC	4 cut(s)	391, 448, 774, 1133
BaeI	ACNNNNGTAYC	2 cut(s)	905, 938
BalI	TGGCCA	1 cut(s)	629
BamHI	GGATCC	2 cut(s)	1504, 1517
BanI	GGYRCC	4 cut(s)	492, 921, 1535, 1567
BanII	GRGCYC	4 cut(s)	95, 222, 405, 1485
BauI	CACGAG	1 cut(s)	500
BbsI	GAAGAC	2 cut(s)	161, 1214
Bbv12I	GWGCWC	6 cut(s)	26, 95, 183, 222, 405, 1089
BbvCI	CCTCAGC	1 cut(s)	518
BbvI	GCAGC	5 cut(s)	17, 20, 23, 26, 565
BccI	CCATC	2 cut(s)	392, 1505
BceAI	ACGGC	4 cut(s)	262, 682, 1216, 1243
BcgI	CGANNNNNNTGC	2 cut(s)	442, 476
BciT130I	CCWGG	3 cut(s)	377, 772, 1077
BciVI	GTATCC	2 cut(s)	922, 1552
BcnI	CCSGG	6 cut(s)	476, 845, 926, 1131, 1374, 1533
BcoDI	GTCTC	2 cut(s)	385, 1446
BfaI	CTAG	1 cut(s)	434
BfoI	RGCGCY	2 cut(s)	993, 1325
BfrI	CTTAAG	1 cut(s)	1198
BfuI	GTATCC	2 cut(s)	922, 1552
BglI	GCCNNNNNGGC	1 cut(s)	255
BisI	GCNGC	9 cut(s)	6, 9, 12, 15, 354, 579, 967, 970, 1008
BlsI	GCNGC	9 cut(s)	7, 10, 13, 16, 355, 580, 968, 971, 1009
Bme1390I	CCNGG	9 cut(s)	377, 476, 772, 845, 926, 1077, 1131, 1374, 1533
Bme18I	GGWCC	4 cut(s)	391, 448, 774, 1133
BmeT110I	CYCGRG	3 cut(s)	917, 1090, 1214
BmgT120I	GGNCC	7 cut(s)	391, 448, 774, 960, 1069, 1133, 1268
BmrFI	CCNGG	9 cut(s)	377, 476, 772, 845, 926, 1077, 1131, 1374, 1533
BpiI	GAAGAC	2 cut(s)	161, 1214
BpmI	CTGGAG	2 cut(s)	10, 359
Bpu10I	CCTNAGC	1 cut(s)	518
Bpu14I	TTCGAA	1 cut(s)	662
BpuMI	CCSGG	6 cut(s)	476, 845, 926, 1131, 1374, 1533
Bsa29I	ATCGAT	1 cut(s)	795
BsaAI	YACGTR	1 cut(s)	762
BsaHI	GRCGYC	5 cut(s)	333, 636, 1064, 1211, 1316
BsaI	GGTCTC	2 cut(s)	385, 1446
BsaJI	CCNNGG	4 cut(s)	228, 630, 770, 1531
BsaWI	WCCGGW	3 cut(s)	594, 1054, 1393
BsaXI	ACNNNNNCTCC	2 cut(s)	404, 434
Bse118I	RCCGGY	3 cut(s)	1054, 1066, 1237
Bse1I	ACTGG	3 cut(s)	27, 210, 958
Bse3DI	GCAATG	2 cut(s)	567, 912
BseBI	CCWGG	3 cut(s)	377, 772, 1077
BseCI	ATCGAT	1 cut(s)	795
BseDI	CCNNGG	4 cut(s)	228, 630, 770, 1531
BseGI	GGATG	3 cut(s)	183, 294, 996
BseMI	GCAATG	2 cut(s)	567, 912
BseMII	CTCAG	3 cut(s)	32, 532, 1457
BseNI	ACTGG	3 cut(s)	27, 210, 958
BseRI	GAGGAG	2 cut(s)	409, 488
BseXI	GCAGC	5 cut(s)	17, 20, 23, 26, 565
BseYI	CCCAGC	4 cut(s)	163, 253, 962, 1136
BsgI	GTGCAG	1 cut(s)	1101
Bsh1236I	CGCG	5 cut(s)	318, 1229, 1310, 1415, 1433
BshFI	GGCC	5 cut(s)	249, 629, 961, 1070, 1270
BshNI	GGYRCC	4 cut(s)	492, 921, 1535, 1567
BshTI	ACCGGT	1 cut(s)	1054
BshVI	ATCGAT	1 cut(s)	795
BsiHKAI	GWGCWC	6 cut(s)	26, 95, 183, 222, 405, 1089
BsiHKCI	CYCGRG	3 cut(s)	917, 1090, 1214
BsiWI	CGTACG	1 cut(s)	1329
BslFI	GGGAC	3 cut(s)	377, 1075, 1119
BsmAI	GTCTC	2 cut(s)	385, 1446
BsmFI	GGGAC	3 cut(s)	377, 1075, 1119
BsnI	GGCC	5 cut(s)	249, 629, 961, 1070, 1270
Bso31I	GGTCTC	2 cut(s)	385, 1446
BsoBI	CYCGRG	3 cut(s)	917, 1090, 1214
Bsp119I	TTCGAA	1 cut(s)	662
Bsp1286I	GDGCHC	7 cut(s)	26, 95, 183, 222, 405, 1089, 1485
Bsp1407I	TGTACA	1 cut(s)	756
Bsp143I	GATC	4 cut(s)	439, 1360, 1504, 1517
Bsp19I	CCATGG	1 cut(s)	630
BspANI	GGCC	5 cut(s)	249, 629, 961, 1070, 1270
BspCNI	CTCAG	3 cut(s)	31, 531, 1456
BspDI	ATCGAT	1 cut(s)	795
BspFNI	CGCG	5 cut(s)	318, 1229, 1310, 1415, 1433
BspPI	GGATC	5 cut(s)	1355, 1499, 1512, 1512, 1525
BspT104I	TTCGAA	1 cut(s)	662
BspT107I	GGYRCC	4 cut(s)	492, 921, 1535, 1567
BspTI	CTTAAG	1 cut(s)	1198
BspTNI	GGTCTC	2 cut(s)	385, 1446
BsrBI	CCGCTC	1 cut(s)	1147
BsrDI	GCAATG	2 cut(s)	567, 912
BsrFI	RCCGGY	3 cut(s)	1054, 1066, 1237
BsrGI	TGTACA	1 cut(s)	756
BsrI	ACTGG	3 cut(s)	27, 210, 958
BssAI	RCCGGY	3 cut(s)	1054, 1066, 1237
BssECI	CCNNGG	4 cut(s)	228, 630, 770, 1531
BssMI	GATC	4 cut(s)	439, 1360, 1504, 1517
BssNI	GRCGYC	5 cut(s)	333, 636, 1064, 1211, 1316
BssSI	CACGAG	1 cut(s)	500
BssT1I	CCWWGG	2 cut(s)	228, 630
Bst2BI	CACGAG	1 cut(s)	500
Bst2UI	CCWGG	3 cut(s)	377, 772, 1077
Bst4CI	ACNGT	1 cut(s)	835
Bst6I	CTCTTC	1 cut(s)	878
BstACI	GRCGYC	5 cut(s)	333, 636, 1064, 1211, 1316
BstAFI	CTTAAG	1 cut(s)	1198
BstAUI	TGTACA	1 cut(s)	756
BstBAI	YACGTR	1 cut(s)	762
BstBI	TTCGAA	1 cut(s)	662
BstC8I	GCNNGC	8 cut(s)	251, 1068, 1336, 1340, 1413, 1417, 1481, 1485
BstDEI	CTNAG	4 cut(s)	18, 518, 814, 1443
BstDSI	CCRYGG	1 cut(s)	630
BstEII	GGTNACC	1 cut(s)	835
BstF5I	GGATG	3 cut(s)	183, 294, 996
BstFNI	CGCG	5 cut(s)	318, 1229, 1310, 1415, 1433
BstH2I	RGCGCY	2 cut(s)	993, 1325
BstHHI	GCGC	6 cut(s)	318, 992, 1231, 1310, 1324, 1433
BstKTI	GATC	4 cut(s)	442, 1363, 1507, 1520
BstMAI	GTCTC	2 cut(s)	385, 1446
BstMBI	GATC	4 cut(s)	439, 1360, 1504, 1517
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 14, 255, 322, 1295, 1412, 1421, 1430
BstNI	CCWGG	3 cut(s)	377, 772, 1077
BstPI	GGTNACC	1 cut(s)	835
BstSCI	CCNGG	9 cut(s)	375, 474, 770, 843, 924, 1075, 1129, 1372, 1531
BstUI	CGCG	5 cut(s)	318, 1229, 1310, 1415, 1433
BstV1I	GCAGC	5 cut(s)	17, 20, 23, 26, 565
BstV2I	GAAGAC	2 cut(s)	161, 1214
BstX2I	RGATCY	2 cut(s)	1504, 1517
BstYI	RGATCY	2 cut(s)	1504, 1517
Bsu15I	ATCGAT	1 cut(s)	795
BsuI	GTATCC	2 cut(s)	922, 1552
BsuRI	GGCC	5 cut(s)	249, 629, 961, 1070, 1270
BsuTUI	ATCGAT	1 cut(s)	795
BtgI	CCRYGG	1 cut(s)	630
BtsCI	GGATG	3 cut(s)	183, 294, 996
BtsI	GCAGTG	1 cut(s)	1089
BtsIMutI	CAGTG	4 cut(s)	26, 217, 366, 1089
Cac8I	GCNNGC	8 cut(s)	251, 1068, 1336, 1340, 1413, 1417, 1481, 1485
CfoI	GCGC	6 cut(s)	318, 992, 1231, 1310, 1324, 1433
Cfr10I	RCCGGY	3 cut(s)	1054, 1066, 1237
Cfr13I	GGNCC	7 cut(s)	391, 448, 774, 960, 1069, 1133, 1268
ClaI	ATCGAT	1 cut(s)	795
CseI	GACGC	6 cut(s)	307, 341, 899, 1072, 1219, 1305
CsiI	ACCWGGT	1 cut(s)	1075
Csp6I	GTAC	8 cut(s)	464, 493, 554, 757, 922, 1186, 1225, 1330
CspAI	ACCGGT	1 cut(s)	1054
CviAII	CATG	5 cut(s)	388, 631, 709, 1282, 1572
CviQI	GTAC	8 cut(s)	464, 493, 554, 757, 922, 1186, 1225, 1330
DdeI	CTNAG	4 cut(s)	18, 518, 814, 1443
DpnI	GATC	4 cut(s)	441, 1362, 1506, 1519
DpnII	GATC	4 cut(s)	439, 1360, 1504, 1517
DraI	TTTAAA	1 cut(s)	1390
DrdI	GACNNNNNNGTC	2 cut(s)	377, 1216
DseDI	GACNNNNNNGTC	2 cut(s)	377, 1216
EaeI	YGGCCR	2 cut(s)	247, 627
Eam1104I	CTCTTC	1 cut(s)	878
EarI	CTCTTC	1 cut(s)	878
EciI	GGCGGA	1 cut(s)	645
Ecl136II	GAGCTC	3 cut(s)	93, 220, 403
Eco130I	CCWWGG	2 cut(s)	228, 630
Eco24I	GRGCYC	4 cut(s)	95, 222, 405, 1485
Eco31I	GGTCTC	2 cut(s)	385, 1446
Eco47I	GGWCC	4 cut(s)	391, 448, 774, 1133
Eco53kI	GAGCTC	3 cut(s)	93, 220, 403
Eco88I	CYCGRG	3 cut(s)	917, 1090, 1214
Eco91I	GGTNACC	1 cut(s)	835
EcoICRI	GAGCTC	3 cut(s)	93, 220, 403
EcoO109I	RGGNCCY	1 cut(s)	1133
EcoO65I	GGTNACC	1 cut(s)	835
EcoRII	CCWGG	3 cut(s)	375, 770, 1075
EcoT14I	CCWWGG	2 cut(s)	228, 630
EcoT38I	GRGCYC	4 cut(s)	95, 222, 405, 1485
ErhI	CCWWGG	2 cut(s)	228, 630
FaeI	CATG	5 cut(s)	391, 634, 712, 1285, 1575
FalI	AAGNNNNNCTT	2 cut(s)	725, 757
FaqI	GGGAC	3 cut(s)	377, 1075, 1119
FatI	CATG	5 cut(s)	387, 630, 708, 1281, 1571
FauI	CCCGC	4 cut(s)	565, 941, 1196, 1472
FblI	GTMKAC	1 cut(s)	750
Fnu4HI	GCNGC	9 cut(s)	6, 9, 12, 15, 354, 579, 967, 970, 1008
FokI	GGATG	3 cut(s)	170, 281, 983
FriOI	GRGCYC	4 cut(s)	95, 222, 405, 1485
Fsp4HI	GCNGC	9 cut(s)	6, 9, 12, 15, 354, 579, 967, 970, 1008
FspBI	CTAG	1 cut(s)	434
GlaI	GCGC	6 cut(s)	317, 991, 1230, 1309, 1323, 1432
GluI	GCNGC	9 cut(s)	6, 9, 12, 15, 354, 579, 967, 970, 1008
GsaI	CCCAGC	4 cut(s)	167, 257, 966, 1140
GsuI	CTGGAG	2 cut(s)	10, 359
HaeII	RGCGCY	2 cut(s)	993, 1325
HaeIII	GGCC	5 cut(s)	249, 629, 961, 1070, 1270
HgaI	GACGC	6 cut(s)	307, 341, 899, 1072, 1219, 1305
HhaI	GCGC	6 cut(s)	318, 992, 1231, 1310, 1324, 1433
Hin1I	GRCGYC	5 cut(s)	333, 636, 1064, 1211, 1316
Hin1II	CATG	5 cut(s)	391, 634, 712, 1285, 1575
Hin6I	GCGC	6 cut(s)	316, 990, 1229, 1308, 1322, 1431
HinP1I	GCGC	6 cut(s)	316, 990, 1229, 1308, 1322, 1431
HincII	GTYRAC	1 cut(s)	751
HindII	GTYRAC	1 cut(s)	751
HinfI	GANTC	7 cut(s)	487, 644, 818, 915, 1217, 1366, 1397
HphI	GGTGA	3 cut(s)	298, 619, 829
Hpy166II	GTNNAC	5 cut(s)	241, 751, 759, 889, 1186
Hpy188I	TCNGA	8 cut(s)	64, 113, 617, 682, 781, 1222, 1305, 1365
Hpy188III	TCNNGA	1 cut(s)	1090
Hpy8I	GTNNAC	5 cut(s)	241, 751, 759, 889, 1186
Hpy99I	CGWCG	9 cut(s)	227, 280, 335, 464, 652, 752, 1249, 1321, 1555
HpyAV	CCTTC	7 cut(s)	43, 368, 478, 669, 1285, 1447, 1549
HpyCH4III	ACNGT	1 cut(s)	835
HpyCH4IV	ACGT	5 cut(s)	459, 636, 671, 761, 1026
HpyCH4V	TGCA	3 cut(s)	1082, 1281, 1524
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 14, 255, 322, 1295, 1412, 1421, 1430
HpyF3I	CTNAG	4 cut(s)	18, 518, 814, 1443
HpySE526I	ACGT	5 cut(s)	459, 636, 671, 761, 1026
Hsp92I	GRCGYC	5 cut(s)	333, 636, 1064, 1211, 1316
Hsp92II	CATG	5 cut(s)	391, 634, 712, 1285, 1575
HspAI	GCGC	6 cut(s)	316, 990, 1229, 1308, 1322, 1431
KflI	GGGWCCC	1 cut(s)	1133
KpnI	GGTACC	2 cut(s)	496, 925
KroI	GCCGGC	1 cut(s)	1066
KroNI	GCCGGC	1 cut(s)	1068
Kzo9I	GATC	4 cut(s)	439, 1360, 1504, 1517
LmnI	GCTCC	7 cut(s)	29, 98, 160, 217, 1092, 1144, 1295
Lsp1109I	GCAGC	5 cut(s)	17, 20, 23, 26, 565
MabI	ACCWGGT	1 cut(s)	1075
MaeI	CTAG	1 cut(s)	434
MaeII	ACGT	5 cut(s)	459, 636, 671, 761, 1026
MaeIII	GTNAC	4 cut(s)	101, 835, 1469, 1511
MalI	GATC	4 cut(s)	441, 1362, 1506, 1519
MbiI	CCGCTC	1 cut(s)	1147
MboI	GATC	4 cut(s)	439, 1360, 1504, 1517
MboII	GAAGA	6 cut(s)	161, 164, 733, 736, 865, 1219
MflI	RGATCY	2 cut(s)	1504, 1517
MhlI	GDGCHC	7 cut(s)	26, 95, 183, 222, 405, 1089, 1485
MlsI	TGGCCA	1 cut(s)	629
MluCI	AATT	3 cut(s)	716, 1037, 1150
MluNI	TGGCCA	1 cut(s)	629
MlyI	GAGTC	2 cut(s)	909, 1226
MmeI	TCCRAC	3 cut(s)	595, 804, 1200
Mox20I	TGGCCA	1 cut(s)	629
MroNI	GCCGGC	1 cut(s)	1066
MscI	TGGCCA	1 cut(s)	629
MseI	TTAA	3 cut(s)	857, 1199, 1389
Msp20I	TGGCCA	1 cut(s)	629
MspA1I	CMGCKG	2 cut(s)	253, 966
MspCI	CTTAAG	1 cut(s)	1198
MspR9I	CCNGG	9 cut(s)	377, 476, 772, 845, 926, 1077, 1131, 1374, 1533
MvaI	CCWGG	3 cut(s)	377, 772, 1077
MvnI	CGCG	5 cut(s)	318, 1229, 1310, 1415, 1433
MwoI	GCNNNNNNNGC	8 cut(s)	11, 14, 255, 322, 1295, 1412, 1421, 1430
NaeI	GCCGGC	1 cut(s)	1068
NciI	CCSGG	6 cut(s)	476, 845, 926, 1131, 1374, 1533
NcoI	CCATGG	1 cut(s)	630
NdeII	GATC	4 cut(s)	439, 1360, 1504, 1517
NgoMIV	GCCGGC	1 cut(s)	1066
NlaIII	CATG	5 cut(s)	391, 634, 712, 1285, 1575
NmuCI	GTSAC	2 cut(s)	101, 835
NspV	TTCGAA	1 cut(s)	662
PcsI	WCGNNNNNNNCGW	3 cut(s)	507, 668, 1325
PdiI	GCCGGC	1 cut(s)	1068
PfeI	GAWTC	5 cut(s)	487, 644, 818, 1366, 1397
Pfl23II	CGTACG	1 cut(s)	1329
PflMI	CCANNNNNTGG	1 cut(s)	886
PinAI	ACCGGT	1 cut(s)	1054
PkrI	GCNGC	9 cut(s)	7, 10, 13, 16, 355, 580, 968, 971, 1009
PleI	GAGTC	2 cut(s)	909, 1225
PpsI	GAGTC	2 cut(s)	909, 1225
Ppu21I	YACGTR	1 cut(s)	762
PpuMI	RGGWCCY	1 cut(s)	1133
Psp124BI	GAGCTC	3 cut(s)	95, 222, 405
Psp5II	RGGWCCY	1 cut(s)	1133
Psp6I	CCWGG	3 cut(s)	375, 770, 1075
PspEI	GGTNACC	1 cut(s)	835
PspFI	CCCAGC	4 cut(s)	163, 253, 962, 1136
PspGI	CCWGG	3 cut(s)	375, 770, 1075
PspLI	CGTACG	1 cut(s)	1329
PspPI	GGNCC	7 cut(s)	391, 448, 774, 960, 1069, 1133, 1268
PspPPI	RGGWCCY	1 cut(s)	1133
PsuI	RGATCY	2 cut(s)	1504, 1517
PvuII	CAGCTG	1 cut(s)	253
RsaI	GTAC	8 cut(s)	465, 494, 555, 758, 923, 1187, 1226, 1331
RsaNI	GTAC	8 cut(s)	464, 493, 554, 757, 922, 1186, 1225, 1330
SacI	GAGCTC	3 cut(s)	95, 222, 405
SalI	GTCGAC	1 cut(s)	749
SaqAI	TTAA	3 cut(s)	857, 1199, 1389
SatI	GCNGC	9 cut(s)	6, 9, 12, 15, 354, 579, 967, 970, 1008
Sau3AI	GATC	4 cut(s)	439, 1360, 1504, 1517
Sau96I	GGNCC	7 cut(s)	391, 448, 774, 960, 1069, 1133, 1268
SchI	GAGTC	2 cut(s)	909, 1226
ScrFI	CCNGG	9 cut(s)	377, 476, 772, 845, 926, 1077, 1131, 1374, 1533
SduI	GDGCHC	7 cut(s)	26, 95, 183, 222, 405, 1089, 1485
SexAI	ACCWGGT	1 cut(s)	1075
SfuI	TTCGAA	1 cut(s)	662
SgrAI	CRCCGGYG	1 cut(s)	1054
SinI	GGWCC	4 cut(s)	391, 448, 774, 1133
SmlI	CTYRAG	1 cut(s)	1198
SmoI	CTYRAG	1 cut(s)	1198
Sse9I	AATT	3 cut(s)	716, 1037, 1150
SspMI	CTAG	1 cut(s)	434
SstI	GAGCTC	3 cut(s)	95, 222, 405
StyD4I	CCNGG	9 cut(s)	375, 474, 770, 843, 924, 1075, 1129, 1372, 1531
StyI	CCWWGG	2 cut(s)	228, 630
TaaI	ACNGT	1 cut(s)	835
TaiI	ACGT	5 cut(s)	462, 639, 674, 764, 1029
TaqI	TCGA	6 cut(s)	642, 662, 750, 795, 876, 1178
TaqII	GACCGA	2 cut(s)	666, 685
TasI	AATT	3 cut(s)	716, 1037, 1150
TatI	WGTACW	1 cut(s)	756
TauI	GCSGC	4 cut(s)	356, 969, 972, 1010
TfiI	GAWTC	5 cut(s)	487, 644, 818, 1366, 1397
Tru1I	TTAA	3 cut(s)	857, 1199, 1389
Tru9I	TTAA	3 cut(s)	857, 1199, 1389
TscAI	CASTG	4 cut(s)	26, 217, 373, 1089
TseFI	GTSAC	2 cut(s)	101, 835
TseI	GCWGC	5 cut(s)	5, 8, 11, 14, 578
Tsp45I	GTSAC	2 cut(s)	101, 835
TspDTI	ATGAA	3 cut(s)	25, 709, 923
TspGWI	ACGGA	3 cut(s)	264, 1157, 1530
TspRI	CASTG	4 cut(s)	26, 217, 373, 1089
Van91I	CCANNNNNTGG	1 cut(s)	886
Vha464I	CTTAAG	1 cut(s)	1198
VpaK11BI	GGWCC	4 cut(s)	391, 448, 774, 1133
XapI	RAATTY	3 cut(s)	716, 1037, 1150
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1054
XmiI	GTMKAC	1 cut(s)	750
XspI	CTAG	1 cut(s)	434
ZraI	GACGTC	1 cut(s)	637

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.