Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0014018

Physical Location & Seq

Genomic Coordinates Reverse (-)

30110401 .. 30112262

Gene Structure 1862 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1172600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGCAGTTACTTCCATGGCCAAGGCGCCTCAGAGCTCTCCTCTTATCCTGCACGGATAAGACCTCCATAGCTAAAGTCCATGCTCTTATGATATTGACTAGTACCCTCGCCGATGGAAGCTTCAACGCTCGACTCATAGCGTCCTACGCGCGTATTGGGGATACTGTGTCCGCCTGCAAAGTGTTCGATAAATTGCCTCAACGAGGCGTGAATGCATGGAATGCCATGATAGTCGCGTATTCTCGGCAAGAGTACCCGTCTCAAGTTTTAAGTTTATATCATCAGATGGTAGCGGATGGAGTTAGACCTGATAGTTCGACTTTTACTGTCGCGATTAAGGCGTGCGCAAGCATGTTGGACTTGGAGATTGGTGAGGAAATTTGGTCTAAGGCGGTGGATTGCGGGTACGAACGTGATGTGTTTGTGGGATCATCTGTTTTGAACTTGTACGCCAAGAGCGGGAAGATGGATGAAGCAGTGGTGGTGTTTGAGAAGATGCCTAGAAAGGACCTTGTTTGTTGGACAACAATGATAACAGGGTTTGTGCAGAGTGGAAGGCCAATGGAAGCAGTTGAGATGTTTAGGGGAATGCAACATGAGGGAATCGAATTTGATGGGGTTTCGATGACGGGGTTGGTACAGGCTTGTGCAAGTCTTGGGGACTTGAGATTGAGTCTTTCTGTTCACGGGTTTATGATTCGGAGAGGTCTTCGTATGGATGTTATGGTTCAAACCAGCCTTGCCCATATGTATGCAAAGAACGGGCATTTGGAGCTAGCTTATAGGGTGTTCGATAGGATGCCTTACAAGAATGCCATTTCGTGGGGTGCTTTGATTTCCGGCTTTGCTCAAAATGGCTTCGCAGGGCGTGCACTCGAAATGCTAGTAGAGATGCAGGGCAGTGGATTTGAACCCGATTCAGCGTGTCTTGTGAGCGCGCTTCTAGCATGTTCACAAGTAGGGTTTTTGAAATGGGGTAAATCCGCACATGCATTTATCTTGAAGAGGCTTGATTTTGATGAAGTTTTGGGTACTGCAGTGATTGACATGTATTCGAAATGCGGATCCCTTTCTTACGCACGTGCTCTCTTCGATCAGATTATTTCCAGAGACTTAATCTCGTGGAATGCTATGATTACAAGCTACGGAATTCATGGTCATGCAGAGGAAGCTCTAGCACTCTTCCTCCAGATGACGGAGACAAACATAAAGCCCGATCATGCTACTTTTGCTTCTCTTCTTTCAGCCTTCAGTCATGCAGGCCTCGTACAAGAAGGCCAGTATTGGTTCGACGTCATGGTCAATAAGTACAAAACCACTCCGGGGGAGAAACATTATGCTTGCATGGTTGACCTACTGGCTCGCGCCGGACGAGTGGAAGAGGCTTATGAGCTAATAAATTCTGTGAATACTGAACGTGGCCTTGCCGTTTGGGTTGCCCTCCTGGCAGGTTGCCACAATCATGGGAAGTCGTCGATTGGAGAGATCGTGGCAAAGAAGATCCTCGATTTGAATCCGGATGACTTAGGAGTTTACGCTTTGGTCTCGAACTTCTACGCAATGGCGAGGAAGTGGGATGATGTAGCAGACATGAAGAAGATGATGAAACAGAGAGGAACAAAGAAAGTGCCTGGTTATAGTGTTGTGGAAGTGAATGGAGAGCTCCATGCTTTTCTCATGGAAGATAAGACACACCAACATCATGGCGATATCATGCAAATCCTGAATAAGTTGGATCATGACATGAGAGCCATCGGATATGTTCCCAGGAATGAATTTCTGTTGCACAATTCGGAAGACGAACATTCCTTCCAGCAAAGAAAATTTATCAAAGTGCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

614

Amino Acids

68.36

Weight (kDa)

6.6

Isoelectric Point (pI)

34.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	70 - 116	5.1e-10	PPR repeat family
PPR_2	PF13041	170 - 217	1.4e-08	PPR repeat family
PPR	PF01535	172 - 202	3.4e-07	PPR repeat
PPR_2	PF13041	371 - 418	1e-10	PPR repeat family
PPR	PF01535	373 - 400	4.8e-06	PPR repeat
E_motif	PF20431	492 - 550	8.4e-16	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014018)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25060
fragaria_vesca	FvH4_2g18670
malus_domestica	MD05G1172600.v1.1
prunus_persica	Prupe.8G200000_v2.0.a1
pyrus_communis	pycom05g15920
rosa_chinensis	RchiOBHm_Chr6g0284231
rosa_laevigata	RLG00000012754
rosa_multiflora	Rmu_sc0002698.1_g000026 Rmu_sc0002698.1_g000027 Rmu_sc0024251.1_g000001
rosa_roxburghii	Rroxscaffold_7G00184760
rosa_rugosa	Rorug06G0162100
rosa_samantha	Rh6AG275000 Rh6BG275500 Rh6CG277200 Rh6DG269900
rosa_wichuraiana	Rw6G023660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1298
AatII	GACGTC	1 cut(s)	1296
Acc16I	TGCGCA	1 cut(s)	346
Acc36I	ACCTGC	1 cut(s)	1439
AccB1I	GGYRCC	1 cut(s)	24
AccB7I	CCANNNNNTGG	1 cut(s)	822
AccBSI	CCGCTC	1 cut(s)	459
AccII	CGCG	6 cut(s)	149, 151, 236, 332, 938, 1365
AccIII	TCCGGA	1 cut(s)	1516
AciI	CCGC	7 cut(s)	171, 293, 392, 402, 459, 984, 1062
AclWI	GGATC	5 cut(s)	436, 1059, 1072, 1495, 1743
AcoI	YGGCCR	1 cut(s)	17
AcsI	RAATTY	6 cut(s)	378, 608, 1149, 1399, 1775, 1823
AcuI	CTGAAG	1 cut(s)	1234
AcvI	CACGTG	1 cut(s)	1082
AcyI	GRCGYC	2 cut(s)	25, 1293
AfaI	GTAC	8 cut(s)	103, 254, 407, 449, 639, 1033, 1269, 1310
AfiI	CCNNNNNNNGG	5 cut(s)	203, 459, 822, 1195, 1323
AflIII	ACRYGT	1 cut(s)	1047
AgsI	TTSAA	7 cut(s)	124, 442, 731, 911, 970, 1003, 1513
AhlI	ACTAGT	1 cut(s)	98
AjnI	CCWGG	3 cut(s)	1443, 1630, 1766
AjuI	GAANNNNNNNTTGG	2 cut(s)	752, 784
AloI	GAACNNNNNNTCC	2 cut(s)	1794, 1826
AluBI	AGCT	9 cut(s)	35, 71, 120, 775, 779, 1143, 1172, 1393, 1663
AluI	AGCT	9 cut(s)	35, 71, 120, 775, 779, 1143, 1172, 1393, 1663
Alw21I	GWGCWC	5 cut(s)	37, 874, 1087, 1665, 1839
Alw26I	GTCTC	4 cut(s)	264, 1104, 1193, 1549
Alw44I	GTGCAC	1 cut(s)	870
AlwI	GGATC	5 cut(s)	436, 1059, 1072, 1495, 1743
Aor13HI	TCCGGA	1 cut(s)	1516
AoxI	GGCC	5 cut(s)	17, 557, 1261, 1276, 1420
ApaLI	GTGCAC	1 cut(s)	870
ApoI	RAATTY	6 cut(s)	378, 608, 1149, 1399, 1775, 1823
AspLEI	GCGC	6 cut(s)	27, 151, 347, 938, 940, 1367
AspS9I	GGNCC	1 cut(s)	508
AsuC2I	CCSGG	1 cut(s)	1323
AsuHPI	GGTGA	1 cut(s)	383
AsuII	TTCGAA	1 cut(s)	1055
AsuNHI	GCTAGC	1 cut(s)	775
AvaII	GGWCC	1 cut(s)	508
BaeGI	GKGCMC	1 cut(s)	874
BaeI	ACNNNNGTAYC	4 cut(s)	397, 430, 1023, 1056
BalI	TGGCCA	1 cut(s)	19
BamHI	GGATCC	1 cut(s)	1064
BanI	GGYRCC	1 cut(s)	24
BanII	GRGCYC	2 cut(s)	37, 1665
BauI	CACGAG	1 cut(s)	1120
BbrPI	CACGTG	1 cut(s)	1082
BbsI	GAAGAC	2 cut(s)	701, 1803
Bbv12I	GWGCWC	5 cut(s)	37, 874, 1087, 1665, 1839
BccI	CCATC	6 cut(s)	107, 280, 290, 460, 608, 1760
BceAI	ACGGC	1 cut(s)	1412
BcgI	CGANNNNNNTGC	2 cut(s)	166, 200
BciT130I	CCWGG	3 cut(s)	1445, 1632, 1768
BciVI	GTATCC	1 cut(s)	154
BcnI	CCSGG	1 cut(s)	1323
BcoDI	GTCTC	4 cut(s)	264, 1104, 1193, 1549
BcuI	ACTAGT	1 cut(s)	98
BfaI	CTAG	6 cut(s)	99, 501, 776, 884, 944, 1175
BfmI	CTRYAG	1 cut(s)	1035
BfoI	RGCGCY	1 cut(s)	28
BfuAI	ACCTGC	1 cut(s)	1439
BfuI	GTATCC	1 cut(s)	154
BglI	GCCNNNNNGGC	1 cut(s)	1445
Bme1390I	CCNGG	4 cut(s)	1323, 1445, 1632, 1768
Bme18I	GGWCC	1 cut(s)	508
BmgT120I	GGNCC	1 cut(s)	508
BmiI	GGNNCC	2 cut(s)	26, 1066
BmrFI	CCNGG	4 cut(s)	1323, 1445, 1632, 1768
BmsI	GCATC	3 cut(s)	486, 789, 882
BmtI	GCTAGC	1 cut(s)	779
BpiI	GAAGAC	2 cut(s)	701, 1803
BplI	GAGNNNNNCTC	2 cut(s)	24, 56
BpmI	CTGGAG	1 cut(s)	1172
Bpu14I	TTCGAA	1 cut(s)	1055
BpuEI	CTTGAG	2 cut(s)	246, 685
BpuMI	CCSGG	1 cut(s)	1323
BsaAI	YACGTR	1 cut(s)	1082
BsaBI	GATNNNNATC	1 cut(s)	1512
BsaHI	GRCGYC	2 cut(s)	25, 1293
BsaI	GGTCTC	1 cut(s)	1549
BsaJI	CCNNGG	4 cut(s)	14, 20, 1322, 1766
BsaWI	WCCGGW	1 cut(s)	1516
BsaXI	ACNNNNNCTCC	4 cut(s)	1170, 1200, 1473, 1503
Bsc4I	CCNNNNNNNGG	5 cut(s)	203, 459, 822, 1195, 1323
Bse1I	ACTGG	2 cut(s)	1279, 1362
Bse3DI	GCAATG	1 cut(s)	1566
Bse8I	GATNNNNATC	1 cut(s)	1512
BseAI	TCCGGA	1 cut(s)	1516
BseBI	CCWGG	3 cut(s)	1445, 1632, 1768
BseDI	CCNNGG	4 cut(s)	14, 20, 1322, 1766
BseGI	GGATG	6 cut(s)	301, 475, 724, 804, 1525, 1582
BseJI	GATNNNNATC	1 cut(s)	1512
BseLI	CCNNNNNNNGG	5 cut(s)	203, 459, 822, 1195, 1323
BseMI	GCAATG	1 cut(s)	1566
BseMII	CTCAG	1 cut(s)	43
BseNI	ACTGG	2 cut(s)	1279, 1362
BsePI	GCGCGC	1 cut(s)	936
BseRI	GAGGAG	1 cut(s)	29
BseSI	GKGCMC	1 cut(s)	874
BsgI	GTGCAG	2 cut(s)	34, 566
Bsh1236I	CGCG	6 cut(s)	149, 151, 236, 332, 938, 1365
BshFI	GGCC	5 cut(s)	19, 559, 1263, 1278, 1422
BshNI	GGYRCC	1 cut(s)	24
BsiHKAI	GWGCWC	5 cut(s)	37, 874, 1087, 1665, 1839
BsiSI	CCGG	4 cut(s)	840, 1322, 1368, 1517
BslFI	GGGAC	1 cut(s)	674
BslI	CCNNNNNNNGG	5 cut(s)	203, 459, 822, 1195, 1323
BsmAI	GTCTC	4 cut(s)	264, 1104, 1193, 1549
BsmBI	CGTCTC	1 cut(s)	264
BsmFI	GGGAC	1 cut(s)	674
BsmI	GAATGC	5 cut(s)	217, 226, 594, 817, 1132
BsnI	GGCC	5 cut(s)	19, 559, 1263, 1278, 1422
Bso31I	GGTCTC	1 cut(s)	1549
Bsp119I	TTCGAA	1 cut(s)	1055
Bsp1286I	GDGCHC	5 cut(s)	37, 874, 1087, 1665, 1839
Bsp13I	TCCGGA	1 cut(s)	1516
Bsp143I	GATC	7 cut(s)	428, 1064, 1093, 1216, 1485, 1500, 1735
Bsp19I	CCATGG	1 cut(s)	14
Bsp68I	TCGCGA	1 cut(s)	332
BspACI	CCGC	7 cut(s)	171, 293, 392, 402, 459, 984, 1062
BspANI	GGCC	5 cut(s)	19, 559, 1263, 1278, 1422
BspCNI	CTCAG	1 cut(s)	42
BspEI	TCCGGA	1 cut(s)	1516
BspFNI	CGCG	6 cut(s)	149, 151, 236, 332, 938, 1365
BspHI	TCATGA	1 cut(s)	1738
BspLI	GGNNCC	2 cut(s)	26, 1066
BspMAI	CTGCAG	1 cut(s)	1039
BspMI	ACCTGC	1 cut(s)	1439
BspOI	GCTAGC	1 cut(s)	779
BspPI	GGATC	5 cut(s)	436, 1059, 1072, 1495, 1743
BspT104I	TTCGAA	1 cut(s)	1055
BspT107I	GGYRCC	1 cut(s)	24
BspTNI	GGTCTC	1 cut(s)	1549
BsrBI	CCGCTC	1 cut(s)	459
BsrDI	GCAATG	1 cut(s)	1566
BsrI	ACTGG	2 cut(s)	1279, 1362
BssECI	CCNNGG	4 cut(s)	14, 20, 1322, 1766
BssHII	GCGCGC	1 cut(s)	936
BssMI	GATC	7 cut(s)	428, 1064, 1093, 1216, 1485, 1500, 1735
BssNI	GRCGYC	2 cut(s)	25, 1293
BssSI	CACGAG	1 cut(s)	1120
BssT1I	CCWWGG	2 cut(s)	14, 20
Bst2BI	CACGAG	1 cut(s)	1120
Bst2UI	CCWGG	3 cut(s)	1445, 1632, 1768
Bst4CI	ACNGT	2 cut(s)	166, 328
Bst6I	CTCTTC	5 cut(s)	998, 1094, 1187, 1242, 1374
BstACI	GRCGYC	2 cut(s)	25, 1293
BstAPI	GCANNNNNTGC	2 cut(s)	221, 869
BstBAI	YACGTR	1 cut(s)	1082
BstBI	TTCGAA	1 cut(s)	1055
BstC8I	GCNNGC	9 cut(s)	175, 343, 349, 777, 870, 938, 1261, 1342, 1363
BstDEI	CTNAG	3 cut(s)	29, 387, 1525
BstDSI	CCRYGG	1 cut(s)	14
BstENI	CCTNNNNNAGG	1 cut(s)	201
BstF5I	GGATG	6 cut(s)	301, 475, 724, 804, 1525, 1582
BstFNI	CGCG	6 cut(s)	149, 151, 236, 332, 938, 1365
BstH2I	RGCGCY	1 cut(s)	28
BstHHI	GCGC	6 cut(s)	27, 151, 347, 938, 940, 1367
BstKTI	GATC	7 cut(s)	431, 1067, 1096, 1219, 1488, 1503, 1738
BstMAI	GTCTC	4 cut(s)	264, 1104, 1193, 1549
BstMBI	GATC	7 cut(s)	428, 1064, 1093, 1216, 1485, 1500, 1735
BstMWI	GCNNNNNNNGC	9 cut(s)	146, 221, 338, 772, 869, 944, 1169, 1229, 1445
BstNI	CCWGG	3 cut(s)	1445, 1632, 1768
BstNSI	RCATGY	4 cut(s)	355, 951, 992, 1051
BstSCI	CCNGG	4 cut(s)	1321, 1443, 1630, 1766
BstSFI	CTRYAG	1 cut(s)	1035
BstSLI	GKGCMC	1 cut(s)	874
BstUI	CGCG	6 cut(s)	149, 151, 236, 332, 938, 1365
BstV2I	GAAGAC	2 cut(s)	701, 1803
BstX2I	RGATCY	2 cut(s)	1064, 1500
BstXI	CCANNNNNNTGG	2 cut(s)	1463, 1703
BstYI	RGATCY	2 cut(s)	1064, 1500
BsuI	GTATCC	1 cut(s)	154
BsuRI	GGCC	5 cut(s)	19, 559, 1263, 1278, 1422
BtgI	CCRYGG	1 cut(s)	14
BtsCI	GGATG	6 cut(s)	301, 475, 724, 804, 1525, 1582
BtsI	GCAGTG	3 cut(s)	483, 907, 1044
BtsIMutI	CAGTG	3 cut(s)	483, 907, 1044
BtuMI	TCGCGA	1 cut(s)	332
BveI	ACCTGC	1 cut(s)	1439
Cac8I	GCNNGC	9 cut(s)	175, 343, 349, 777, 870, 938, 1261, 1342, 1363
CciI	TCATGA	1 cut(s)	1738
CfoI	GCGC	6 cut(s)	27, 151, 347, 938, 940, 1367
Cfr13I	GGNCC	1 cut(s)	508
CseI	GACGC	1 cut(s)	129
Csp6I	GTAC	8 cut(s)	102, 253, 406, 448, 638, 1032, 1268, 1309
CviQI	GTAC	8 cut(s)	102, 253, 406, 448, 638, 1032, 1268, 1309
DdeI	CTNAG	3 cut(s)	29, 387, 1525
DinI	GGCGCC	1 cut(s)	26
DpnI	GATC	7 cut(s)	430, 1066, 1095, 1218, 1487, 1502, 1737
DpnII	GATC	7 cut(s)	428, 1064, 1093, 1216, 1485, 1500, 1735
DrdI	GACNNNNNNGTC	1 cut(s)	1298
DseDI	GACNNNNNNGTC	1 cut(s)	1298
EaeI	YGGCCR	1 cut(s)	17
Eam1104I	CTCTTC	5 cut(s)	998, 1094, 1187, 1242, 1374
EarI	CTCTTC	5 cut(s)	998, 1094, 1187, 1242, 1374
EciI	GGCGGA	1 cut(s)	160
Ecl136II	GAGCTC	2 cut(s)	35, 1663
Eco130I	CCWWGG	2 cut(s)	14, 20
Eco147I	AGGCCT	1 cut(s)	1263
Eco24I	GRGCYC	2 cut(s)	37, 1665
Eco31I	GGTCTC	1 cut(s)	1549
Eco32I	GATATC	1 cut(s)	1711
Eco47I	GGWCC	1 cut(s)	508
Eco53kI	GAGCTC	2 cut(s)	35, 1663
Eco57I	CTGAAG	1 cut(s)	1234
Eco72I	CACGTG	1 cut(s)	1082
EcoICRI	GAGCTC	2 cut(s)	35, 1663
EcoNI	CCTNNNNNAGG	1 cut(s)	201
EcoO109I	RGGNCCY	1 cut(s)	508
EcoRI	GAATTC	1 cut(s)	1149
EcoRII	CCWGG	3 cut(s)	1443, 1630, 1766
EcoRV	GATATC	1 cut(s)	1711
EcoT14I	CCWWGG	2 cut(s)	14, 20
EcoT22I	ATGCAT	2 cut(s)	217, 994
EcoT38I	GRGCYC	2 cut(s)	37, 1665
EgeI	GGCGCC	1 cut(s)	26
EheI	GGCGCC	1 cut(s)	26
ErhI	CCWWGG	2 cut(s)	14, 20
Esp3I	CGTCTC	1 cut(s)	264
FaqI	GGGAC	1 cut(s)	674
FauI	CCCGC	2 cut(s)	395, 452
FauNDI	CATATG	1 cut(s)	747
FokI	GGATG	6 cut(s)	308, 482, 731, 811, 1532, 1589
FriOI	GRGCYC	2 cut(s)	37, 1665
FspBI	CTAG	6 cut(s)	99, 501, 776, 884, 944, 1175
FspI	TGCGCA	1 cut(s)	346
GlaI	GCGC	6 cut(s)	26, 150, 346, 937, 939, 1366
GsuI	CTGGAG	1 cut(s)	1172
HaeII	RGCGCY	1 cut(s)	28
HaeIII	GGCC	5 cut(s)	19, 559, 1263, 1278, 1422
HapII	CCGG	4 cut(s)	840, 1322, 1368, 1517
HgaI	GACGC	1 cut(s)	129
HhaI	GCGC	6 cut(s)	27, 151, 347, 938, 940, 1367
Hin1I	GRCGYC	2 cut(s)	25, 1293
Hin6I	GCGC	6 cut(s)	25, 149, 345, 936, 938, 1365
HinP1I	GCGC	6 cut(s)	25, 149, 345, 936, 938, 1365
HincII	GTYRAC	1 cut(s)	1351
HindII	GTYRAC	1 cut(s)	1351
HindIII	AAGCTT	1 cut(s)	118
HinfI	GANTC	6 cut(s)	132, 603, 673, 697, 917, 1513
HpaII	CCGG	4 cut(s)	840, 1322, 1368, 1517
HphI	GGTGA	1 cut(s)	383
Hpy166II	GTNNAC	5 cut(s)	685, 872, 953, 1351, 1534
Hpy188I	TCNGA	6 cut(s)	32, 285, 702, 1098, 1757, 1795
Hpy188III	TCNNGA	8 cut(s)	331, 1000, 1107, 1189, 1517, 1546, 1723, 1739
Hpy8I	GTNNAC	5 cut(s)	685, 872, 953, 1351, 1534
Hpy99I	CGWCG	2 cut(s)	1295, 1477
HpyAV	CCTTC	4 cut(s)	549, 1258, 1268, 1819
HpyCH4III	ACNGT	2 cut(s)	166, 328
HpyCH4IV	ACGT	4 cut(s)	412, 1081, 1293, 1417
HpyF10VI	GCNNNNNNNGC	9 cut(s)	146, 221, 338, 772, 869, 944, 1169, 1229, 1445
HpyF3I	CTNAG	3 cut(s)	29, 387, 1525
HpySE526I	ACGT	4 cut(s)	412, 1081, 1293, 1417
Hsp92I	GRCGYC	2 cut(s)	25, 1293
HspAI	GCGC	6 cut(s)	25, 149, 345, 936, 938, 1365
KasI	GGCGCC	1 cut(s)	24
Kpn2I	TCCGGA	1 cut(s)	1516
Kzo9I	GATC	7 cut(s)	428, 1064, 1093, 1216, 1485, 1500, 1735
LmnI	GCTCC	2 cut(s)	772, 1668
LweI	GCATC	3 cut(s)	486, 789, 882
MaeI	CTAG	6 cut(s)	99, 501, 776, 884, 944, 1175
MaeII	ACGT	4 cut(s)	412, 1081, 1293, 1417
MaeIII	GTNAC	1 cut(s)	6
MalI	GATC	7 cut(s)	430, 1066, 1095, 1218, 1487, 1502, 1737
MbiI	CCGCTC	1 cut(s)	459
MboI	GATC	7 cut(s)	428, 1064, 1093, 1216, 1485, 1500, 1735
MflI	RGATCY	2 cut(s)	1064, 1500
MhlI	GDGCHC	5 cut(s)	37, 874, 1087, 1665, 1839
MlsI	TGGCCA	1 cut(s)	19
MluCI	AATT	8 cut(s)	191, 378, 608, 1149, 1399, 1775, 1789, 1823
MluNI	TGGCCA	1 cut(s)	19
Mly113I	GGCGCC	1 cut(s)	25
MlyI	GAGTC	2 cut(s)	126, 682
MmeI	TCCRAC	3 cut(s)	336, 500, 1713
Mox20I	TGGCCA	1 cut(s)	19
Mph1103I	ATGCAT	2 cut(s)	217, 994
MroI	TCCGGA	1 cut(s)	1516
MscI	TGGCCA	1 cut(s)	19
MseI	TTAA	3 cut(s)	269, 336, 1115
MslI	CAYNNNNRTG	3 cut(s)	750, 1158, 1461
Msp20I	TGGCCA	1 cut(s)	19
MspI	CCGG	4 cut(s)	840, 1322, 1368, 1517
MspR9I	CCNGG	4 cut(s)	1323, 1445, 1632, 1768
Mva1269I	GAATGC	5 cut(s)	217, 226, 594, 817, 1132
MvaI	CCWGG	3 cut(s)	1445, 1632, 1768
MvnI	CGCG	6 cut(s)	149, 151, 236, 332, 938, 1365
MwoI	GCNNNNNNNGC	9 cut(s)	146, 221, 338, 772, 869, 944, 1169, 1229, 1445
NarI	GGCGCC	1 cut(s)	25
NciI	CCSGG	1 cut(s)	1323
NcoI	CCATGG	1 cut(s)	14
NdeI	CATATG	1 cut(s)	747
NdeII	GATC	7 cut(s)	428, 1064, 1093, 1216, 1485, 1500, 1735
NheI	GCTAGC	1 cut(s)	775
NlaIV	GGNNCC	2 cut(s)	26, 1066
NmeAIII	GCCGAG	1 cut(s)	223
NruI	TCGCGA	1 cut(s)	332
NsbI	TGCGCA	1 cut(s)	346
NsiI	ATGCAT	2 cut(s)	217, 994
NspI	RCATGY	4 cut(s)	355, 951, 992, 1051
NspV	TTCGAA	1 cut(s)	1055
PagI	TCATGA	1 cut(s)	1738
PauI	GCGCGC	1 cut(s)	936
PceI	AGGCCT	1 cut(s)	1263
PciI	ACATGT	1 cut(s)	1047
PcsI	WCGNNNNNNNCGW	1 cut(s)	1369
PctI	GAATGC	5 cut(s)	217, 226, 594, 817, 1132
PfeI	GAWTC	4 cut(s)	603, 697, 917, 1513
PflMI	CCANNNNNTGG	1 cut(s)	822
PleI	GAGTC	2 cut(s)	126, 681
PluTI	GGCGCC	1 cut(s)	28
PmaCI	CACGTG	1 cut(s)	1082
PmlI	CACGTG	1 cut(s)	1082
PpsI	GAGTC	2 cut(s)	126, 681
Ppu21I	YACGTR	1 cut(s)	1082
PpuMI	RGGWCCY	1 cut(s)	508
PscI	ACATGT	1 cut(s)	1047
Psp124BI	GAGCTC	2 cut(s)	37, 1665
Psp5II	RGGWCCY	1 cut(s)	508
Psp6I	CCWGG	3 cut(s)	1443, 1630, 1766
PspCI	CACGTG	1 cut(s)	1082
PspGI	CCWGG	3 cut(s)	1443, 1630, 1766
PspN4I	GGNNCC	2 cut(s)	26, 1066
PspPI	GGNCC	1 cut(s)	508
PspPPI	RGGWCCY	1 cut(s)	508
PstI	CTGCAG	1 cut(s)	1039
PsuI	RGATCY	2 cut(s)	1064, 1500
PteI	GCGCGC	1 cut(s)	936
RruI	TCGCGA	1 cut(s)	332
RsaI	GTAC	8 cut(s)	103, 254, 407, 449, 639, 1033, 1269, 1310
RsaNI	GTAC	8 cut(s)	102, 253, 406, 448, 638, 1032, 1268, 1309
RseI	CAYNNNNRTG	3 cut(s)	750, 1158, 1461
SacI	GAGCTC	2 cut(s)	37, 1665
SaqAI	TTAA	3 cut(s)	269, 336, 1115
Sau3AI	GATC	7 cut(s)	428, 1064, 1093, 1216, 1485, 1500, 1735
Sau96I	GGNCC	1 cut(s)	508
SchI	GAGTC	2 cut(s)	126, 682
ScrFI	CCNGG	4 cut(s)	1323, 1445, 1632, 1768
SduI	GDGCHC	5 cut(s)	37, 874, 1087, 1665, 1839
SfaNI	GCATC	3 cut(s)	486, 789, 882
SfcI	CTRYAG	1 cut(s)	1035
SfoI	GGCGCC	1 cut(s)	26
SfuI	TTCGAA	1 cut(s)	1055
SinI	GGWCC	1 cut(s)	508
SmiMI	CAYNNNNRTG	3 cut(s)	750, 1158, 1461
SmlI	CTYRAG	2 cut(s)	261, 664
SmoI	CTYRAG	2 cut(s)	261, 664
SpeI	ACTAGT	1 cut(s)	98
Sse9I	AATT	8 cut(s)	191, 378, 608, 1149, 1399, 1775, 1789, 1823
SseBI	AGGCCT	1 cut(s)	1263
SsiI	CCGC	7 cut(s)	171, 293, 392, 402, 459, 984, 1062
SspDI	GGCGCC	1 cut(s)	24
SspMI	CTAG	6 cut(s)	99, 501, 776, 884, 944, 1175
SstI	GAGCTC	2 cut(s)	37, 1665
StuI	AGGCCT	1 cut(s)	1263
StyD4I	CCNGG	4 cut(s)	1321, 1443, 1630, 1766
StyI	CCWWGG	2 cut(s)	14, 20
TaaI	ACNGT	2 cut(s)	166, 328
TaiI	ACGT	4 cut(s)	415, 1084, 1296, 1420
TasI	AATT	8 cut(s)	191, 378, 608, 1149, 1399, 1775, 1789, 1823
TatI	WGTACW	1 cut(s)	1308
TfiI	GAWTC	4 cut(s)	603, 697, 917, 1513
Tru1I	TTAA	3 cut(s)	269, 336, 1115
Tru9I	TTAA	3 cut(s)	269, 336, 1115
TscAI	CASTG	3 cut(s)	483, 907, 1044
TspDTI	ATGAA	6 cut(s)	486, 1035, 1142, 1607, 1619, 1788
TspGWI	ACGGA	3 cut(s)	68, 1161, 1211
TspRI	CASTG	3 cut(s)	483, 907, 1044
Van91I	CCANNNNNTGG	1 cut(s)	822
VneI	GTGCAC	1 cut(s)	870
VpaK11BI	GGWCC	1 cut(s)	508
XagI	CCTNNNNNAGG	1 cut(s)	201
XapI	RAATTY	6 cut(s)	378, 608, 1149, 1399, 1775, 1823
XceI	RCATGY	4 cut(s)	355, 951, 992, 1051
XspI	CTAG	6 cut(s)	99, 501, 776, 884, 944, 1175
ZraI	GACGTC	1 cut(s)	1294
Zsp2I	ATGCAT	2 cut(s)	217, 994

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.