Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0011392

Physical Location & Seq

Genomic Coordinates Forward (+)

30447369 .. 30450921

Gene Structure 3553 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1177400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1800 bp

ATGGTGAAGAAAAGCAAAAAGAGCAAGAGTAAGAGAGTGACGTTGAGTAAGAAGTACAAGGTGATAAGGAAGGTGAAGGAGCACCACAAAAAGAAGGCCAAGGACGCCAAGAAGCTCGGCTTCAAGAACAAGAAGGTCGAGAAGGACCCGGGTATTCCTAATGACTGGCCCTTCAAGGAGCAGGAGCTCAAGGCTCTCGAAGTCCGTCGGGCTAAATATATCGAAGAAGAAGAGCAAAAGAAGGCTGCCCGCAAAGAAAGGGCTAAAAAGAGGAAGATGGGGATTTTAGAGGACGACGATGTCCCCAAGTCAGAGGAAGTGGCAGAAGAAGAGAGTATTGATGATTCCACTGCCTTCGGGAATAATAGAGATAGCTCAGATAGGGCTTTCTACAAGGAGTTGGTTAAAGTTATTGAAGCCTCAGATGTCATTTTGGAAGTTCTTGATGCTCGAGACCCCCTGGGTACCCGATGTATTGATATGGAAAAGATGGTGATGAAATCGGGTCCTAACAAGCATCTCGTCTTACTTCTGAACAAAATTGATCTTGTCCCACGAGAAGCTGCTGAGAAATGGCTGACTTATCTTAGGGAAGAGTTACCAGCTGTTGCCTTCAAGTGCAACACCCAAGAGCAAAGGTCAAACTTAGGGTGGAAATCATCTTCGAAGAAATCAAAACCAAGCAGCCTTCTCCAGACAAGCGACTGTCTGGGAGCTGAAACTCTTCTAAAATTGTTGAAGAACTATTCCAGAAGTCATGAGATCAAGAAATCAATTACTGTGGGTGTTATCGGCCTTCCCAATGTCGGAAAGAGTAGTCTGATTAATAGTTTGAAGAGAAGCCGTGTTGTCAATGTTGGTTCTACTCCAGGATTAACAAGATCACTGCAAGAAGTTCAGTTGGATAATAATGTCAAATTGCTGGACTGCCCTGGTGTTGTAATGCTTAGATCTAAAGAGAATGATGCCTCTATAGCACTACGAAACTGCAAAAGAATTGAGAAGTTAGAGGATCCAATTGCTCCAGTAAAGGAAATTTTGAAGCTTTGCCCAGAAAGACTTTTGGTGACTCTATACAAGCTCCCAAGTTTCGATTCAGTTGATGACTTTCTGCATAAGGTGGCTACAGTTAGGGGTAAGCTAAAAAAGGGTGGTGTTGTAGATATTGGTGCTGCCGCAAGAATTGTGCTGCACGACTGGAACGAGGGTAAAATTCCATACTACACTATGCCCCCAGTTAGGAATCAAGATCATCCTTCAGAGGCAAACATTGTTTCGCAGCTGGGCAAGGCGTTTAACATTGATGAAGTATACAATGCAGAATCTTCATTCATCGGAAGCCTCAAGTCTGTTAGTGATTTCCATCCTGTCGAAGTTCCTCCCAGTTGCCCGCTCAATTTCAATGAAGATATTGAAGAGGTTGATACGGCAGAAGGACCATCAAGTCATGGTGATGGAAGTCCTGAAGATATGGTTGACGGAGACGATGACCAGTCCATGGCATGTGAAGAAGATGATGGTGGCAAACCCAAGGAAAAGAAAGCTATTAGAACTATCAGGCAAAATGAGAAATTGTACACAGCTGAAGGTATTCTCAATCCTAAGCTAAAGAAAGCAGAAAGGAAGAAAAAGAAAAAGGCCAAGTCAGCCGCAGCCTCAGTCGACGCTATGGATGCTGACGGTGACTATGACTTTAAAGTGGATTACAAGAACAAGGGATCCGCTATGGATGTTAGTGATGGTCAACTTATAGGAAAGGTTCCGATGTCTGGTACTGGATTTGATGAACCGGAGAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003924 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005911 GO:0006355 GO:0007275 GO:0008150 GO:0009506 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0010073 GO:0010074 GO:0010077 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0019827 GO:0022414 GO:0022613 GO:0030054 GO:0030856 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0042254 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045595 GO:0045604 GO:0045682 GO:0045892 GO:0045934 GO:0045995 GO:0048316 GO:0048366 GO:0048367 GO:0048437 GO:0048444 GO:0048507 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0055044 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071840 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0098727 GO:0099402 GO:1902679 GO:1903506 GO:1903507 GO:1905392 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

GN3L_Grn1 MMR_HSR1

Protein Analysis

600

Amino Acids

66.87

Weight (kDa)

8.93

Isoelectric Point (pI)

46.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GN3L_Grn1	PF08701	16 - 90	8.4e-19	GNL3L/Grn1 putative GTPase
MMR_HSR1	PF01926	260 - 325	1.7e-15	50S ribosome-binding GTPase
FeoB_N	PF02421	260 - 327	1.8e-08	Ferrous iron transport protein B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011392)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07050
fragaria_vesca	FvH4_3g29200 FvH4_3g29200
malus_domestica	MD05G1177400.v1.1 MD10G1165800.v1.1
prunus_persica	Prupe.8G204300_v2.0.a1
pyrus_communis	pycom05g16330 pycom05g16380 pycom10g14300
rosa_chinensis	RchiOBHm_Chr6g0285031
rosa_laevigata	RLG00000012684
rosa_multiflora	Rmu_sc0002478.1_g000012
rosa_roxburghii	Rroxscaffold_7G00183820 Rroxscaffold_7G00184090
rosa_rugosa	Rorug06G0170100
rosa_samantha	Rh6AG281800 Rh6BG282400 Rh6CG284100 Rh6DG277300
rosa_wichuraiana	Rw6G024210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	299, 1443
Acc65I	GGTACC	1 cut(s)	464
AccB1I	GGYRCC	1 cut(s)	464
AccB7I	CCANNNNNTGG	1 cut(s)	1498
AccBSI	CCGCTC	1 cut(s)	1393
AccI	GTMKAC	2 cut(s)	1311, 1662
AciI	CCGC	5 cut(s)	250, 1176, 1391, 1650, 1722
AclWI	GGATC	4 cut(s)	1007, 1020, 1713, 1726
AcsI	RAATTY	2 cut(s)	1035, 1212
AcuI	CTGAAG	3 cut(s)	1242, 1485, 1605
AcyI	GRCGYC	1 cut(s)	105
AfaI	GTAC	4 cut(s)	56, 466, 1577, 1774
AfiI	CCNNNNNNNGG	4 cut(s)	806, 1239, 1498, 1769
AgsI	TTSAA	9 cut(s)	124, 175, 416, 616, 739, 835, 1042, 1402, 1415
AjnI	CCWGG	3 cut(s)	459, 868, 931
AjuI	GAANNNNNNNTTGG	2 cut(s)	1078, 1110
AloI	GAACNNNNNNTCC	2 cut(s)	1703, 1735
Alw21I	GWGCWC	2 cut(s)	84, 189
Alw26I	GTCTC	2 cut(s)	447, 1476
AlwI	GGATC	4 cut(s)	1007, 1020, 1713, 1726
Ama87I	CYCGRG	2 cut(s)	148, 450
AoxI	GGCC	4 cut(s)	96, 167, 793, 1638
ApeKI	GCWGC	7 cut(s)	245, 563, 684, 1172, 1189, 1279, 1652
ApoI	RAATTY	2 cut(s)	1035, 1212
AseI	ATTAAT	1 cut(s)	825
Asp700I	GAANNNNTTC	2 cut(s)	723, 1590
Asp718I	GGTACC	1 cut(s)	464
AspS9I	GGNCC	4 cut(s)	145, 168, 506, 1436
AsuC2I	CCSGG	2 cut(s)	149, 150
AsuHPI	GGTGA	7 cut(s)	16, 73, 85, 505, 1078, 1463, 1694
AsuII	TTCGAA	1 cut(s)	665
AvaI	CYCGRG	2 cut(s)	148, 450
AvaII	GGWCC	3 cut(s)	145, 506, 1436
BamHI	GGATCC	2 cut(s)	1012, 1718
BanI	GGYRCC	1 cut(s)	464
BanII	GRGCYC	1 cut(s)	189
BauI	CACGAG	1 cut(s)	555
Bbv12I	GWGCWC	2 cut(s)	84, 189
BbvI	GCAGC	7 cut(s)	232, 550, 696, 1159, 1176, 1291, 1664
BccI	CCATC	7 cut(s)	271, 484, 1371, 1447, 1448, 1511, 1733
BceAI	ACGGC	2 cut(s)	828, 1443
BciT130I	CCWGG	3 cut(s)	461, 870, 933
BcnI	CCSGG	2 cut(s)	149, 150
BcoDI	GTCTC	2 cut(s)	447, 1476
BfmI	CTRYAG	2 cut(s)	972, 1125
BglII	AGATCT	1 cut(s)	950
BisI	GCNGC	9 cut(s)	246, 564, 685, 1173, 1176, 1190, 1280, 1650, 1653
BlsI	GCNGC	9 cut(s)	247, 565, 686, 1174, 1177, 1191, 1281, 1651, 1654
Bme1390I	CCNGG	5 cut(s)	149, 150, 461, 870, 933
Bme18I	GGWCC	3 cut(s)	145, 506, 1436
BmeT110I	CYCGRG	2 cut(s)	148, 450
BmgT120I	GGNCC	4 cut(s)	145, 168, 506, 1436
BmiI	GGNNCC	6 cut(s)	147, 466, 507, 1014, 1720, 1761
BmrFI	CCNGG	5 cut(s)	149, 150, 461, 870, 933
BmrI	ACTGGG	2 cut(s)	1229, 1377
BmsI	GCATC	4 cut(s)	436, 526, 955, 1663
BmuI	ACTGGG	2 cut(s)	1229, 1377
BpmI	CTGGAG	3 cut(s)	677, 852, 1008
Bpu10I	CCTNAGC	1 cut(s)	1602
Bpu14I	TTCGAA	1 cut(s)	665
BpuEI	CTTGAG	2 cut(s)	173, 1328
BpuMI	CCSGG	2 cut(s)	149, 150
BsaBI	GATNNNNATC	1 cut(s)	1362
BsaHI	GRCGYC	1 cut(s)	105
BsaI	GGTCTC	1 cut(s)	447
BsaJI	CCNNGG	7 cut(s)	99, 148, 459, 460, 931, 1497, 1530
BsaWI	WCCGGW	1 cut(s)	1789
Bsc4I	CCNNNNNNNGG	4 cut(s)	806, 1239, 1498, 1769
Bse1I	ACTGG	7 cut(s)	170, 1025, 1202, 1235, 1383, 1492, 1780
Bse8I	GATNNNNATC	1 cut(s)	1362
BseBI	CCWGG	3 cut(s)	461, 870, 933
BseDI	CCNNGG	7 cut(s)	99, 148, 459, 460, 931, 1497, 1530
BseGI	GGATG	4 cut(s)	1252, 1363, 1678, 1735
BseJI	GATNNNNATC	1 cut(s)	1362
BseLI	CCNNNNNNNGG	4 cut(s)	806, 1239, 1498, 1769
BseMII	CTCAG	4 cut(s)	390, 435, 558, 1671
BseNI	ACTGG	7 cut(s)	170, 1025, 1202, 1235, 1383, 1492, 1780
BseXI	GCAGC	7 cut(s)	232, 550, 696, 1159, 1176, 1291, 1664
BseYI	CCCAGC	1 cut(s)	1282
BsgI	GTGCAG	1 cut(s)	1175
BshFI	GGCC	4 cut(s)	98, 169, 795, 1640
BshNI	GGYRCC	1 cut(s)	464
BsiHKAI	GWGCWC	2 cut(s)	84, 189
BsiHKCI	CYCGRG	2 cut(s)	148, 450
BsiSI	CCGG	2 cut(s)	149, 1790
BslFI	GGGAC	2 cut(s)	287, 536
BslI	CCNNNNNNNGG	4 cut(s)	806, 1239, 1498, 1769
BsmAI	GTCTC	2 cut(s)	447, 1476
BsmBI	CGTCTC	1 cut(s)	1476
BsmFI	GGGAC	2 cut(s)	287, 536
BsnI	GGCC	4 cut(s)	98, 169, 795, 1640
Bso31I	GGTCTC	1 cut(s)	447
BsoBI	CYCGRG	2 cut(s)	148, 450
Bsp119I	TTCGAA	1 cut(s)	665
Bsp1286I	GDGCHC	2 cut(s)	84, 189
Bsp1407I	TGTACA	1 cut(s)	1575
Bsp143I	GATC	7 cut(s)	544, 762, 881, 950, 1012, 1249, 1718
Bsp19I	CCATGG	1 cut(s)	1497
BspACI	CCGC	5 cut(s)	250, 1176, 1391, 1650, 1722
BspANI	GGCC	4 cut(s)	98, 169, 795, 1640
BspCNI	CTCAG	4 cut(s)	389, 434, 559, 1670
BspHI	TCATGA	1 cut(s)	757
BspLI	GGNNCC	6 cut(s)	147, 466, 507, 1014, 1720, 1761
BspPI	GGATC	4 cut(s)	1007, 1020, 1713, 1726
BspQI	GCTCTTC	1 cut(s)	225
BspT104I	TTCGAA	1 cut(s)	665
BspT107I	GGYRCC	1 cut(s)	464
BspTNI	GGTCTC	1 cut(s)	447
BsrBI	CCGCTC	1 cut(s)	1393
BsrGI	TGTACA	1 cut(s)	1575
BsrI	ACTGG	7 cut(s)	170, 1025, 1202, 1235, 1383, 1492, 1780
BssECI	CCNNGG	7 cut(s)	99, 148, 459, 460, 931, 1497, 1530
BssMI	GATC	7 cut(s)	544, 762, 881, 950, 1012, 1249, 1718
BssNAI	GTATAC	1 cut(s)	1312
BssNI	GRCGYC	1 cut(s)	105
BssSI	CACGAG	1 cut(s)	555
BssT1I	CCWWGG	3 cut(s)	99, 1497, 1530
Bst1107I	GTATAC	1 cut(s)	1312
Bst2BI	CACGAG	1 cut(s)	555
Bst2UI	CCWGG	3 cut(s)	461, 870, 933
Bst4CI	ACNGT	4 cut(s)	707, 781, 1129, 1682
Bst6I	CTCTTC	6 cut(s)	225, 324, 588, 729, 830, 1410
BstACI	GRCGYC	1 cut(s)	105
BstAUI	TGTACA	1 cut(s)	1575
BstBI	TTCGAA	1 cut(s)	665
BstC8I	GCNNGC	2 cut(s)	250, 1391
BstDEI	CTNAG	8 cut(s)	376, 421, 567, 587, 646, 947, 1602, 1657
BstDSI	CCRYGG	1 cut(s)	1497
BstF5I	GGATG	4 cut(s)	1252, 1363, 1678, 1735
BstKTI	GATC	7 cut(s)	547, 765, 884, 953, 1015, 1252, 1721
BstMAI	GTCTC	2 cut(s)	447, 1476
BstMBI	GATC	7 cut(s)	544, 762, 881, 950, 1012, 1249, 1718
BstMWI	GCNNNNNNNGC	5 cut(s)	21, 104, 974, 1646, 1673
BstNI	CCWGG	3 cut(s)	461, 870, 933
BstNSI	RCATGY	1 cut(s)	1506
BstSCI	CCNGG	5 cut(s)	147, 148, 459, 868, 931
BstSFI	CTRYAG	2 cut(s)	972, 1125
BstV1I	GCAGC	7 cut(s)	232, 550, 696, 1159, 1176, 1291, 1664
BstX2I	RGATCY	3 cut(s)	950, 1012, 1718
BstYI	RGATCY	3 cut(s)	950, 1012, 1718
BstZ17I	GTATAC	1 cut(s)	1312
BsuRI	GGCC	4 cut(s)	98, 169, 795, 1640
BtgI	CCRYGG	1 cut(s)	1497
BtsCI	GGATG	4 cut(s)	1252, 1363, 1678, 1735
BtsI	GCAGTG	2 cut(s)	348, 884
BtsIMutI	CAGTG	2 cut(s)	348, 884
Cac8I	GCNNGC	2 cut(s)	250, 1391
CciI	TCATGA	1 cut(s)	757
Cfr13I	GGNCC	4 cut(s)	145, 168, 506, 1436
Cfr9I	CCCGGG	1 cut(s)	148
CseI	GACGC	2 cut(s)	113, 1673
Csp6I	GTAC	4 cut(s)	55, 465, 1576, 1773
CspCI	CAANNNNNGTGG	2 cut(s)	762, 797
CviAII	CATG	4 cut(s)	758, 1448, 1498, 1503
CviQI	GTAC	4 cut(s)	55, 465, 1576, 1773
DdeI	CTNAG	8 cut(s)	376, 421, 567, 587, 646, 947, 1602, 1657
DpnI	GATC	7 cut(s)	546, 764, 883, 952, 1014, 1251, 1720
DpnII	GATC	7 cut(s)	544, 762, 881, 950, 1012, 1249, 1718
DraI	TTTAAA	1 cut(s)	1696
DrdI	GACNNNNNNGTC	2 cut(s)	299, 1443
DseDI	GACNNNNNNGTC	2 cut(s)	299, 1443
Eam1104I	CTCTTC	6 cut(s)	225, 324, 588, 729, 830, 1410
EarI	CTCTTC	6 cut(s)	225, 324, 588, 729, 830, 1410
Ecl136II	GAGCTC	1 cut(s)	187
Eco130I	CCWWGG	3 cut(s)	99, 1497, 1530
Eco24I	GRGCYC	1 cut(s)	189
Eco31I	GGTCTC	1 cut(s)	447
Eco47I	GGWCC	3 cut(s)	145, 506, 1436
Eco53kI	GAGCTC	1 cut(s)	187
Eco57I	CTGAAG	3 cut(s)	1242, 1485, 1605
Eco88I	CYCGRG	2 cut(s)	148, 450
EcoICRI	GAGCTC	1 cut(s)	187
EcoO109I	RGGNCCY	2 cut(s)	145, 506
EcoRII	CCWGG	3 cut(s)	459, 868, 931
EcoT14I	CCWWGG	3 cut(s)	99, 1497, 1530
EcoT38I	GRGCYC	1 cut(s)	189
ErhI	CCWWGG	3 cut(s)	99, 1497, 1530
Esp3I	CGTCTC	1 cut(s)	1476
FaeI	CATG	4 cut(s)	761, 1451, 1501, 1506
FalI	AAGNNNNNCTT	2 cut(s)	104, 136
FaqI	GGGAC	2 cut(s)	287, 536
FatI	CATG	4 cut(s)	757, 1447, 1497, 1502
FauI	CCCGC	2 cut(s)	257, 1398
FblI	GTMKAC	2 cut(s)	1311, 1662
Fnu4HI	GCNGC	9 cut(s)	246, 564, 685, 1173, 1176, 1190, 1280, 1650, 1653
FokI	GGATG	4 cut(s)	1239, 1350, 1685, 1742
FriOI	GRGCYC	1 cut(s)	189
Fsp4HI	GCNGC	9 cut(s)	246, 564, 685, 1173, 1176, 1190, 1280, 1650, 1653
GluI	GCNGC	9 cut(s)	246, 564, 685, 1173, 1176, 1190, 1280, 1650, 1653
GsaI	CCCAGC	1 cut(s)	1286
GsuI	CTGGAG	3 cut(s)	677, 852, 1008
HaeIII	GGCC	4 cut(s)	98, 169, 795, 1640
HapII	CCGG	2 cut(s)	149, 1790
HgaI	GACGC	2 cut(s)	113, 1673
Hin1I	GRCGYC	1 cut(s)	105
Hin1II	CATG	4 cut(s)	761, 1451, 1501, 1506
HincII	GTYRAC	3 cut(s)	1477, 1663, 1745
HindII	GTYRAC	3 cut(s)	1477, 1663, 1745
HindIII	AAGCTT	1 cut(s)	1043
HinfI	GANTC	5 cut(s)	344, 1069, 1094, 1243, 1322
HpaII	CCGG	2 cut(s)	149, 1790
HphI	GGTGA	7 cut(s)	16, 73, 85, 505, 1078, 1463, 1694
Hpy166II	GTNNAC	5 cut(s)	1312, 1477, 1578, 1663, 1745
Hpy188I	TCNGA	9 cut(s)	313, 379, 424, 534, 809, 822, 1261, 1337, 1764
Hpy8I	GTNNAC	5 cut(s)	1312, 1477, 1578, 1663, 1745
Hpy99I	CGWCG	3 cut(s)	210, 299, 1667
HpyCH4III	ACNGT	4 cut(s)	707, 781, 1129, 1682
HpyCH4IV	ACGT	1 cut(s)	41
HpyCH4V	TGCA	6 cut(s)	621, 889, 990, 1114, 1192, 1319
HpyF10VI	GCNNNNNNNGC	5 cut(s)	21, 104, 974, 1646, 1673
HpyF3I	CTNAG	8 cut(s)	376, 421, 567, 587, 646, 947, 1602, 1657
HpySE526I	ACGT	1 cut(s)	41
Hsp92I	GRCGYC	1 cut(s)	105
Hsp92II	CATG	4 cut(s)	761, 1451, 1501, 1506
KpnI	GGTACC	1 cut(s)	468
Kzo9I	GATC	7 cut(s)	544, 762, 881, 950, 1012, 1249, 1718
LguI	GCTCTTC	1 cut(s)	225
LmnI	GCTCC	6 cut(s)	79, 178, 184, 713, 1027, 1086
Lsp1109I	GCAGC	7 cut(s)	232, 550, 696, 1159, 1176, 1291, 1664
LweI	GCATC	4 cut(s)	436, 526, 955, 1663
MaeII	ACGT	1 cut(s)	41
MaeIII	GTNAC	4 cut(s)	37, 597, 1066, 1682
MalI	GATC	7 cut(s)	546, 764, 883, 952, 1014, 1251, 1720
MbiI	CCGCTC	1 cut(s)	1393
MboI	GATC	7 cut(s)	544, 762, 881, 950, 1012, 1249, 1718
MfeI	CAATTG	1 cut(s)	1017
MflI	RGATCY	3 cut(s)	950, 1012, 1718
MhlI	GDGCHC	2 cut(s)	84, 189
MlyI	GAGTC	1 cut(s)	1063
MmeI	TCCRAC	2 cut(s)	787, 882
MroXI	GAANNNNTTC	2 cut(s)	723, 1590
MseI	TTAA	5 cut(s)	405, 825, 875, 1296, 1695
MslI	CAYNNNNRTG	1 cut(s)	1452
MspA1I	CMGCKG	3 cut(s)	605, 1282, 1583
MspI	CCGG	2 cut(s)	149, 1790
MspR9I	CCNGG	5 cut(s)	149, 150, 461, 870, 933
MunI	CAATTG	1 cut(s)	1017
MvaI	CCWGG	3 cut(s)	461, 870, 933
MwoI	GCNNNNNNNGC	5 cut(s)	21, 104, 974, 1646, 1673
NciI	CCSGG	2 cut(s)	149, 150
NcoI	CCATGG	1 cut(s)	1497
NdeII	GATC	7 cut(s)	544, 762, 881, 950, 1012, 1249, 1718
NlaIII	CATG	4 cut(s)	761, 1451, 1501, 1506
NlaIV	GGNNCC	6 cut(s)	147, 466, 507, 1014, 1720, 1761
NmeAIII	GCCGAG	1 cut(s)	96
NmuCI	GTSAC	3 cut(s)	37, 1066, 1682
NspI	RCATGY	1 cut(s)	1506
NspV	TTCGAA	1 cut(s)	665
PaeR7I	CTCGAG	1 cut(s)	450
PagI	TCATGA	1 cut(s)	757
PasI	CCCWGGG	1 cut(s)	460
PciSI	GCTCTTC	1 cut(s)	225
PcsI	WCGNNNNNNNCGW	1 cut(s)	1200
PdmI	GAANNNNTTC	2 cut(s)	723, 1590
PfeI	GAWTC	4 cut(s)	344, 1094, 1243, 1322
PflFI	GACNNNGTC	1 cut(s)	299
PflMI	CCANNNNNTGG	1 cut(s)	1498
PfoI	TCCNGGA	1 cut(s)	868
PkrI	GCNGC	9 cut(s)	247, 565, 686, 1174, 1177, 1191, 1281, 1651, 1654
PleI	GAGTC	1 cut(s)	1063
PpsI	GAGTC	1 cut(s)	1063
PpuMI	RGGWCCY	2 cut(s)	145, 506
PshBI	ATTAAT	1 cut(s)	825
Psp124BI	GAGCTC	1 cut(s)	189
Psp5II	RGGWCCY	2 cut(s)	145, 506
Psp6I	CCWGG	3 cut(s)	459, 868, 931
PspFI	CCCAGC	1 cut(s)	1282
PspGI	CCWGG	3 cut(s)	459, 868, 931
PspN4I	GGNNCC	6 cut(s)	147, 466, 507, 1014, 1720, 1761
PspPI	GGNCC	4 cut(s)	145, 168, 506, 1436
PspPPI	RGGWCCY	2 cut(s)	145, 506
PsuI	RGATCY	3 cut(s)	950, 1012, 1718
PsyI	GACNNNGTC	1 cut(s)	299
PvuII	CAGCTG	3 cut(s)	605, 1282, 1583
RsaI	GTAC	4 cut(s)	56, 466, 1577, 1774
RsaNI	GTAC	4 cut(s)	55, 465, 1576, 1773
RseI	CAYNNNNRTG	1 cut(s)	1452
SacI	GAGCTC	1 cut(s)	189
SalI	GTCGAC	1 cut(s)	1661
SapI	GCTCTTC	1 cut(s)	225
SaqAI	TTAA	5 cut(s)	405, 825, 875, 1296, 1695
SatI	GCNGC	9 cut(s)	246, 564, 685, 1173, 1176, 1190, 1280, 1650, 1653
Sau3AI	GATC	7 cut(s)	544, 762, 881, 950, 1012, 1249, 1718
Sau96I	GGNCC	4 cut(s)	145, 168, 506, 1436
SchI	GAGTC	1 cut(s)	1063
ScrFI	CCNGG	5 cut(s)	149, 150, 461, 870, 933
SduI	GDGCHC	2 cut(s)	84, 189
SfaNI	GCATC	4 cut(s)	436, 526, 955, 1663
SfcI	CTRYAG	2 cut(s)	972, 1125
Sfr274I	CTCGAG	1 cut(s)	450
SfuI	TTCGAA	1 cut(s)	665
SinI	GGWCC	3 cut(s)	145, 506, 1436
SlaI	CTCGAG	1 cut(s)	450
SmaI	CCCGGG	1 cut(s)	150
SmiMI	CAYNNNNRTG	1 cut(s)	1452
SmlI	CTYRAG	3 cut(s)	188, 450, 1343
SmoI	CTYRAG	3 cut(s)	188, 450, 1343
SsiI	CCGC	5 cut(s)	250, 1176, 1391, 1650, 1722
SstI	GAGCTC	1 cut(s)	189
StyD4I	CCNGG	5 cut(s)	147, 148, 459, 868, 931
StyI	CCWWGG	3 cut(s)	99, 1497, 1530
TaaI	ACNGT	4 cut(s)	707, 781, 1129, 1682
TaiI	ACGT	1 cut(s)	44
TaqI	TCGA	8 cut(s)	138, 198, 222, 451, 665, 1092, 1371, 1662
TatI	WGTACW	2 cut(s)	54, 1575
TauI	GCSGC	2 cut(s)	1178, 1652
TfiI	GAWTC	4 cut(s)	344, 1094, 1243, 1322
Tru1I	TTAA	5 cut(s)	405, 825, 875, 1296, 1695
Tru9I	TTAA	5 cut(s)	405, 825, 875, 1296, 1695
TscAI	CASTG	2 cut(s)	355, 891
TseFI	GTSAC	3 cut(s)	37, 1066, 1682
TseI	GCWGC	7 cut(s)	245, 563, 684, 1172, 1189, 1279, 1652
Tsp45I	GTSAC	3 cut(s)	37, 1066, 1682
TspDTI	ATGAA	6 cut(s)	512, 1317, 1320, 1321, 1419, 1800
TspGWI	ACGGA	2 cut(s)	194, 1494
TspMI	CCCGGG	1 cut(s)	148
TspRI	CASTG	2 cut(s)	355, 891
Tth111I	GACNNNGTC	1 cut(s)	299
Van91I	CCANNNNNTGG	1 cut(s)	1498
VpaK11BI	GGWCC	3 cut(s)	145, 506, 1436
VspI	ATTAAT	1 cut(s)	825
XapI	RAATTY	2 cut(s)	1035, 1212
XceI	RCATGY	1 cut(s)	1506
XhoI	CTCGAG	1 cut(s)	450
XmaI	CCCGGG	1 cut(s)	148
XmiI	GTMKAC	2 cut(s)	1311, 1662
XmnI	GAANNNNTTC	2 cut(s)	723, 1590

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.