MD05G1179600.v1.1

Kinase-like protein TMKL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015402
Physical Location & Seq
Reverse (-)
30738166 .. 30741110
2945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1179600.v1.1.491

Sequence Viewer

Length: 2016 bp
ATGGAGGTTTCGAAACGTTTCTCTCTCTACATTTTCCTCCTCTGCTTCCTCCTACCCTTAAACAGCACCCCCACTGAGTCTGTTTCTGCAGATGTCGAGCTTCTCCTGGGAAAAATCAAAGCTTCACTGCAAGGTAACTCTCAGAACTTGCTCTTATCTTCCTGGAACTCCTCTGTGCCTCTCTGCCAATGGAGGGGACTCAAATGGGTTTTCTCCAACGGCTCTCTTTTGCTCTGCACCGACCTATCCTCCCCCCAATGGACCAATTTGTCTCTCTCCAGAGACCCATCTCTCCACCTTTTCTCCCTTCAGCTCCCATCCGCCAATCTCTCTGGTTCGCTCCCCCGAGAGCTGGAGGAGTTCTCCATGCTCCAAAGTTTGTACCTTAACATCAATTTCCTCAGTGGGACCGTCCCATTGGAGCTTGGGTACAGCTCTTCCCTCTCTGACATTGATTTGGGAGGAAATTTGTTGAATGGGGTTTTGCCACCTTCGATTTGGAACTTGTGTGACAATCTGGTTTCGCTTCGGGTTCATGCGAATTCGCTATCCGGGTCGGTTCCTGAACCCGCATTGCGAAACTCTAGCTGCAAGAATTTTCAGTTTCTTGATTTTGGTGGGAACAAGTTTTCTGGGAATTTCCCAGAATTTGTAACCCAGTTTGGTGGGCTTAAAGAGCTTGATCTTGGGAGTAACATGTTTTCGGGTCCGATTCCTGAGGGTTTAGCGCAGTTAAACCTCGAAAAATTGAACCTTTCACATAATAATTTTAGTGGGGTGTTGCCTGTTTTTGGTGGATCAAAGTTTGGTGTGGAGGCCTTTGAGGGTAACAACCCTGGACTTTGTGGGGCACCTTTGAAAAGTTGCAGTGGAAGCTCTGGGTTGAGTCCCGGGGCAATTGCCGGCATTGTTATCGGTTTGATGACGGCAACTGTGGTTTTGGCTTCATTGTGTATTGGATATGTGCAAAACAAGAAGAAAAACAGCAGGGAAGAGAGTGAAGATGAGTTGGAGGAAGAAGAAGATGAGGAAAGTGGCGGCGGTGGGGGTGGTGATCAAGGGAGGCTTATTTTGTTTCAGGGCGGTGAGCATTTGACGTTGGAGGATGTGTTGAATGCCACAGGACAAGTTTTGGAGAAGACGAATTATGGGACTCTTTACAAGGCAAAGCTTTCAGATGGAGTGGCCATTACTTTGAGGTTGTTGAGGGAAGGTAGTTGCAAGGATGGGAGCTCTTGTCTTCATGTGGTGAAACAATTGGGGAGGATTCGGCATGAGAATTTGATTCCGCTGAGAGCTTTCTACCAGGGGAAGAGAGGGGAGAAGCTTCTGATATATGACCATCTTCCTCACAGAAGCCTTCATGATCTTTTACATGAAACCAGAGCAGGGAAACCTGTGCTGAACTGGGCTCGGCGACACAAGATTGCACTGGGAATAGCTCGAGGACTAGCATATCTTCATACCGGTCTTGAAACACCTATCACTCATGGGACAGTAAAATCTAAGAATGTGCTAGTGGATGAGTTTTCTGTAGCTAGGCTCACAGAATATGGGCTTGACAAGCTAATGATCCCTACAGTAGCTGATGAAATGGTGGCGGTTGCAAAGAATGACGGTTACAAGGCACCAGAGCTTCAAAAGATGAAGAAATGTAGTTCTAGGACAGATGTTTATGCATTTGGAATTCTTCTGTTGGAAATTTTGATAGGCAAGAAGCCTGGTAAAACCGGGAGGAGTGGAGAATTTGTGGATTTGCCTTCGATGGTGAAAGTTGCAGTCTTGGAGGAGACAACACTGGAGGTTTTCGATGTGGAGGTACTGAAGGGGGTGAGGAGTCCCATGGAAGAAGGTCTGTTTCAGGCTCTGAAGCTCGCAATGGGTTGCTGTGCACCGGTTGCATCGGTTAGGCCCTCCATGGATGAAGTTGTGAAGCAGTTGGAGGAGAACAGGCCAAGGAATAGGTCTACTTTGTACAGCCCCACAGAGACGAGTGAGATTGGTACCCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

672

Amino Acids

72.93

Weight (kDa)

5.9

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 375 - 647 6.8e-24 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 383 - 645 1.4e-20 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24660
fragaria_vesca FvH4_2g19550
malus_domestica MD05G1179600.v1.1 MD10G1169200.v1.1
prunus_persica Prupe.8G206900_v2.0.a1
pyrus_communis pycom05g16610 pycom10g14600
rosa_chinensis RchiOBHm_Chr6g0285321
rosa_laevigata RLG00000012654
rosa_multiflora Rmu_sc0011958.1_g000002
rosa_roxburghii Rroxscaffold_7G00183360
rosa_rugosa Rorug06G0173500
rosa_samantha Rh6BG285000 Rh6CG286500 Rh6DG279500
rosa_wichuraiana Rw6G024460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 268
Acc65I GGTACC 1 cut(s) 2003
AccB1I GGYRCC 3 cut(s) 850, 1627, 2003
AccI GTMKAC 1 cut(s) 1967
AciI CCGC 7 cut(s) 321, 570, 1038, 1041, 1083, 1289, 1601
AclI AACGTT 1 cut(s) 16
AclWI GGATC 2 cut(s) 805, 1567
AcoI YGGCCR 1 cut(s) 1185
AcsI RAATTY 9 cut(s) 466, 541, 595, 637, 647, 1279, 1686, 1701, 1745
AcuI CTGAAG 3 cut(s) 293, 1844, 1889
AfaI GTAC 5 cut(s) 383, 431, 1821, 1976, 2005
AfiI CCNNNNNNNGG 5 cut(s) 193, 258, 352, 791, 1389
AflIII ACRYGT 1 cut(s) 696
AgeI ACCGGT 2 cut(s) 1466, 1894
AgsI TTSAA 6 cut(s) 475, 751, 859, 1114, 1475, 1640
AjnI CCWGG 5 cut(s) 105, 161, 835, 1305, 1720
Alw21I GWGCWC 2 cut(s) 1235, 1894
Alw26I GTCTC 4 cut(s) 276, 276, 1784, 1982
Alw44I GTGCAC 1 cut(s) 1890
AlwI GGATC 2 cut(s) 805, 1567
AlwNI CAGNNNCTG 2 cut(s) 1586, 1867
Ama87I CYCGRG 3 cut(s) 345, 890, 1443
AoxI GGCC 4 cut(s) 816, 1185, 1910, 1952
ApaLI GTGCAC 1 cut(s) 1890
ApeKI GCWGC 1 cut(s) 588
ApoI RAATTY 9 cut(s) 466, 541, 595, 637, 647, 1279, 1686, 1701, 1745
AsiGI ACCGGT 2 cut(s) 1466, 1894
Asp700I GAANNNNTTC 1 cut(s) 17
Asp718I GGTACC 1 cut(s) 2003
AspLEI GCGC 1 cut(s) 730
AspS9I GGNCC 4 cut(s) 261, 408, 707, 1911
AsuC2I CCSGG 4 cut(s) 553, 891, 892, 1732
AsuHPI GGTGA 5 cut(s) 1064, 1097, 1261, 1780, 1843
AsuII TTCGAA 1 cut(s) 11
AvaI CYCGRG 3 cut(s) 345, 890, 1443
AvaII GGWCC 3 cut(s) 261, 408, 707
AxyI CCTNAGG 1 cut(s) 717
BaeGI GKGCMC 2 cut(s) 853, 1894
BalI TGGCCA 1 cut(s) 1187
BanI GGYRCC 3 cut(s) 850, 1627, 2003
BanII GRGCYC 2 cut(s) 1235, 1414
BbsI GAAGAC 2 cut(s) 1145, 1232
Bbv12I GWGCWC 2 cut(s) 1235, 1894
BbvI GCAGC 1 cut(s) 575
BccI CCATC 6 cut(s) 295, 325, 1172, 1220, 1350, 1759
BceAI ACGGC 2 cut(s) 235, 942
BcgI CGANNNNNNTGC 2 cut(s) 895, 929
BciT130I CCWGG 5 cut(s) 107, 163, 837, 1307, 1722
BclI TGATCA 1 cut(s) 1054
BcnI CCSGG 4 cut(s) 553, 891, 892, 1732
BcoDI GTCTC 4 cut(s) 276, 276, 1784, 1982
BfaI CTAG 5 cut(s) 585, 1451, 1517, 1539, 1662
BfmI CTRYAG 3 cut(s) 87, 1533, 1578
BisI GCNGC 2 cut(s) 589, 1039
BlsI GCNGC 2 cut(s) 590, 1040
Bme1390I CCNGG 9 cut(s) 107, 163, 553, 837, 891, 892, 1307, 1722, 1732
Bme18I GGWCC 3 cut(s) 261, 408, 707
BmeT110I CYCGRG 3 cut(s) 345, 890, 1443
BmgT120I GGNCC 4 cut(s) 261, 408, 707, 1911
BmiI GGNNCC 6 cut(s) 409, 561, 708, 852, 1629, 2005
BmrFI CCNGG 9 cut(s) 107, 163, 553, 837, 891, 892, 1307, 1722, 1732
BmrI ACTGGG 3 cut(s) 652, 1417, 1442
BmsI GCATC 1 cut(s) 1910
BmuI ACTGGG 3 cut(s) 652, 1417, 1442
BpiI GAAGAC 2 cut(s) 1145, 1232
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 3 cut(s) 262, 374, 1820
Bpu14I TTCGAA 1 cut(s) 11
BpuMI CCSGG 4 cut(s) 553, 891, 892, 1732
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 8 cut(s) 106, 835, 890, 891, 1306, 1842, 1917, 1955
BsaWI WCCGGW 2 cut(s) 1466, 1894
Bsc4I CCNNNNNNNGG 5 cut(s) 193, 258, 352, 791, 1389
Bse118I RCCGGY 3 cut(s) 902, 1466, 1894
Bse1I ACTGG 4 cut(s) 658, 1412, 1437, 1803
Bse21I CCTNAGG 1 cut(s) 717
Bse3DI GCAATG 2 cut(s) 572, 1884
BseBI CCWGG 5 cut(s) 107, 163, 837, 1307, 1722
BseDI CCNNGG 8 cut(s) 106, 835, 890, 891, 1306, 1842, 1917, 1955
BseGI GGATG 5 cut(s) 317, 1111, 1231, 1528, 1927
BseLI CCNNNNNNNGG 5 cut(s) 193, 258, 352, 791, 1389
BseMI GCAATG 2 cut(s) 572, 1884
BseMII CTCAG 5 cut(s) 66, 155, 415, 708, 1283
BseNI ACTGG 4 cut(s) 658, 1412, 1437, 1803
BseRI GAGGAG 7 cut(s) 29, 160, 371, 1750, 1802, 1849, 1958
BseSI GKGCMC 2 cut(s) 853, 1894
BseXI GCAGC 1 cut(s) 575
BsgI GTGCAG 1 cut(s) 220
BshFI GGCC 4 cut(s) 818, 1187, 1912, 1954
BshNI GGYRCC 3 cut(s) 850, 1627, 2003
BshTI ACCGGT 2 cut(s) 1466, 1894
BsiHKAI GWGCWC 2 cut(s) 1235, 1894
BsiHKCI CYCGRG 3 cut(s) 345, 890, 1443
BsiSI CCGG 6 cut(s) 552, 891, 903, 1467, 1731, 1895
BslFI GGGAC 7 cut(s) 210, 398, 421, 873, 1165, 1507, 1824
BslI CCNNNNNNNGG 5 cut(s) 193, 258, 352, 791, 1389
BsmAI GTCTC 4 cut(s) 276, 276, 1784, 1982
BsmBI CGTCTC 1 cut(s) 1982
BsmFI GGGAC 7 cut(s) 210, 398, 421, 873, 1165, 1507, 1824
BsmI GAATGC 1 cut(s) 1120
BsnI GGCC 4 cut(s) 818, 1187, 1912, 1954
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 3 cut(s) 345, 890, 1443
Bsp119I TTCGAA 1 cut(s) 11
Bsp1286I GDGCHC 4 cut(s) 853, 1235, 1414, 1894
Bsp1407I TGTACA 1 cut(s) 1974
Bsp143I GATC 5 cut(s) 682, 797, 1054, 1366, 1572
Bsp19I CCATGG 2 cut(s) 1842, 1917
BspACI CCGC 7 cut(s) 321, 570, 1038, 1041, 1083, 1289, 1601
BspANI GGCC 4 cut(s) 818, 1187, 1912, 1954
BspCNI CTCAG 5 cut(s) 67, 154, 414, 709, 1284
BspHI TCATGA 1 cut(s) 1363
BspLI GGNNCC 6 cut(s) 409, 561, 708, 852, 1629, 2005
BspMAI CTGCAG 1 cut(s) 91
BspPI GGATC 2 cut(s) 805, 1567
BspQI GCTCTTC 1 cut(s) 442
BspT104I TTCGAA 1 cut(s) 11
BspT107I GGYRCC 3 cut(s) 850, 1627, 2003
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 2 cut(s) 572, 1884
BsrFI RCCGGY 3 cut(s) 902, 1466, 1894
BsrGI TGTACA 1 cut(s) 1974
BsrI ACTGG 4 cut(s) 658, 1412, 1437, 1803
BssAI RCCGGY 3 cut(s) 902, 1466, 1894
BssECI CCNNGG 8 cut(s) 106, 835, 890, 891, 1306, 1842, 1917, 1955
BssMI GATC 5 cut(s) 682, 797, 1054, 1366, 1572
BssT1I CCWWGG 3 cut(s) 1842, 1917, 1955
Bst2UI CCWGG 5 cut(s) 107, 163, 837, 1307, 1722
Bst4CI ACNGT 5 cut(s) 412, 934, 1498, 1582, 1619
Bst6I CTCTTC 3 cut(s) 442, 987, 1307
BstAPI GCANNNNNTGC 1 cut(s) 1898
BstAUI TGTACA 1 cut(s) 1974
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 904, 1875
BstDEI CTNAG 6 cut(s) 75, 141, 401, 717, 1292, 1506
BstDSI CCRYGG 2 cut(s) 1842, 1917
BstF5I GGATG 5 cut(s) 317, 1111, 1231, 1528, 1927
BstHHI GCGC 1 cut(s) 730
BstKTI GATC 5 cut(s) 685, 800, 1057, 1369, 1575
BstMAI GTCTC 4 cut(s) 276, 276, 1784, 1982
BstMBI GATC 5 cut(s) 682, 797, 1054, 1366, 1572
BstMWI GCNNNNNNNGC 4 cut(s) 676, 873, 1564, 1898
BstNI CCWGG 5 cut(s) 107, 163, 837, 1307, 1722
BstNSI RCATGY 1 cut(s) 700
BstSCI CCNGG 9 cut(s) 105, 161, 551, 835, 889, 890, 1305, 1720, 1730
BstSFI CTRYAG 3 cut(s) 87, 1533, 1578
BstSLI GKGCMC 2 cut(s) 853, 1894
BstV1I GCAGC 1 cut(s) 575
BstV2I GAAGAC 2 cut(s) 1145, 1232
BstXI CCANNNNNNTGG 1 cut(s) 665
Bsu36I CCTNAGG 1 cut(s) 717
BsuRI GGCC 4 cut(s) 818, 1187, 1912, 1954
BtgI CCRYGG 2 cut(s) 1842, 1917
BtsCI GGATG 5 cut(s) 317, 1111, 1231, 1528, 1927
BtsI GCAGTG 2 cut(s) 125, 874
BtsIMutI CAGTG 6 cut(s) 72, 125, 409, 874, 1430, 1796
Cac8I GCNNGC 2 cut(s) 904, 1875
CaiI CAGNNNCTG 2 cut(s) 1586, 1867
CciI TCATGA 1 cut(s) 1363
CfoI GCGC 1 cut(s) 730
Cfr10I RCCGGY 3 cut(s) 902, 1466, 1894
Cfr13I GGNCC 4 cut(s) 261, 408, 707, 1911
Cfr9I CCCGGG 1 cut(s) 890
Csp6I GTAC 5 cut(s) 382, 430, 1820, 1975, 2004
CspAI ACCGGT 2 cut(s) 1466, 1894
CviQI GTAC 5 cut(s) 382, 430, 1820, 1975, 2004
DdeI CTNAG 6 cut(s) 75, 141, 401, 717, 1292, 1506
DpnI GATC 5 cut(s) 684, 799, 1056, 1368, 1574
DpnII GATC 5 cut(s) 682, 797, 1054, 1366, 1572
DrdI GACNNNNNNGTC 1 cut(s) 268
DseDI GACNNNNNNGTC 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 1185
Eam1104I CTCTTC 3 cut(s) 442, 987, 1307
EarI CTCTTC 3 cut(s) 442, 987, 1307
EciI GGCGGA 1 cut(s) 310
Ecl136II GAGCTC 1 cut(s) 1233
Eco130I CCWWGG 3 cut(s) 1842, 1917, 1955
Eco147I AGGCCT 1 cut(s) 818
Eco24I GRGCYC 2 cut(s) 1235, 1414
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 3 cut(s) 261, 408, 707
Eco53kI GAGCTC 1 cut(s) 1233
Eco57I CTGAAG 3 cut(s) 293, 1844, 1889
Eco81I CCTNAGG 1 cut(s) 717
Eco88I CYCGRG 3 cut(s) 345, 890, 1443
EcoICRI GAGCTC 1 cut(s) 1233
EcoO109I RGGNCCY 1 cut(s) 1911
EcoRI GAATTC 2 cut(s) 541, 1686
EcoRII CCWGG 5 cut(s) 105, 161, 835, 1305, 1720
EcoT14I CCWWGG 3 cut(s) 1842, 1917, 1955
EcoT22I ATGCAT 1 cut(s) 1681
EcoT38I GRGCYC 2 cut(s) 1235, 1414
ErhI CCWWGG 3 cut(s) 1842, 1917, 1955
Esp3I CGTCTC 1 cut(s) 1982
FalI AAGNNNNNCTT 2 cut(s) 1050, 1082
FaqI GGGAC 7 cut(s) 210, 398, 421, 873, 1165, 1507, 1824
FauI CCCGC 1 cut(s) 577
FbaI TGATCA 1 cut(s) 1054
FblI GTMKAC 1 cut(s) 1967
Fnu4HI GCNGC 2 cut(s) 589, 1039
FokI GGATG 5 cut(s) 304, 1118, 1238, 1535, 1934
FriOI GRGCYC 2 cut(s) 1235, 1414
Fsp4HI GCNGC 2 cut(s) 589, 1039
FspBI CTAG 5 cut(s) 585, 1451, 1517, 1539, 1662
GlaI GCGC 1 cut(s) 729
GluI GCNGC 2 cut(s) 589, 1039
GsuI CTGGAG 3 cut(s) 262, 374, 1820
HaeIII GGCC 4 cut(s) 818, 1187, 1912, 1954
HapII CCGG 6 cut(s) 552, 891, 903, 1467, 1731, 1895
HhaI GCGC 1 cut(s) 730
Hin6I GCGC 1 cut(s) 728
HinP1I GCGC 1 cut(s) 728
HindIII AAGCTT 3 cut(s) 120, 1169, 1325
HinfI GANTC 8 cut(s) 77, 198, 712, 886, 1153, 1267, 1285, 1837
HpaII CCGG 6 cut(s) 552, 891, 903, 1467, 1731, 1895
HphI GGTGA 5 cut(s) 1064, 1097, 1261, 1780, 1843
Hpy166II GTNNAC 2 cut(s) 1892, 1968
Hpy188I TCNGA 6 cut(s) 144, 448, 711, 1177, 1332, 1869
Hpy188III TCNNGA 6 cut(s) 279, 563, 608, 716, 1364, 1472
Hpy8I GTNNAC 2 cut(s) 1892, 1968
HpyAV CCTTC 7 cut(s) 317, 501, 1205, 1370, 1770, 1819, 1844
HpyCH4III ACNGT 5 cut(s) 412, 934, 1498, 1582, 1619
HpyCH4IV ACGT 2 cut(s) 16, 1097
HpyF10VI GCNNNNNNNGC 4 cut(s) 676, 873, 1564, 1898
HpyF3I CTNAG 6 cut(s) 75, 141, 401, 717, 1292, 1506
HpySE526I ACGT 2 cut(s) 16, 1097
HspAI GCGC 1 cut(s) 728
KpnI GGTACC 1 cut(s) 2007
KroI GCCGGC 1 cut(s) 902
KroNI GCCGGC 1 cut(s) 904
Ksp22I TGATCA 1 cut(s) 1054
Kzo9I GATC 5 cut(s) 682, 797, 1054, 1366, 1572
LguI GCTCTTC 1 cut(s) 442
LmnI GCTCC 5 cut(s) 318, 345, 375, 421, 1230
Lsp1109I GCAGC 1 cut(s) 575
LweI GCATC 1 cut(s) 1910
MaeI CTAG 5 cut(s) 585, 1451, 1517, 1539, 1662
MaeII ACGT 2 cut(s) 16, 1097
MaeIII GTNAC 6 cut(s) 134, 509, 652, 692, 827, 1619
MalI GATC 5 cut(s) 684, 799, 1056, 1368, 1574
MboI GATC 5 cut(s) 682, 797, 1054, 1366, 1572
MfeI CAATTG 2 cut(s) 897, 1256
MhlI GDGCHC 4 cut(s) 853, 1235, 1414, 1894
MlsI TGGCCA 1 cut(s) 1187
MluNI TGGCCA 1 cut(s) 1187
MlyI GAGTC 5 cut(s) 86, 192, 895, 1147, 1846
MmeI TCCRAC 5 cut(s) 240, 990, 1080, 1677, 1920
Mox20I TGGCCA 1 cut(s) 1187
Mph1103I ATGCAT 1 cut(s) 1681
MroNI GCCGGC 1 cut(s) 902
MroXI GAANNNNTTC 1 cut(s) 17
MscI TGGCCA 1 cut(s) 1187
MseI TTAA 4 cut(s) 59, 387, 672, 734
Msp20I TGGCCA 1 cut(s) 1187
MspA1I CMGCKG 1 cut(s) 1291
MspI CCGG 6 cut(s) 552, 891, 903, 1467, 1731, 1895
MspR9I CCNGG 9 cut(s) 107, 163, 553, 837, 891, 892, 1307, 1722, 1732
MunI CAATTG 2 cut(s) 897, 1256
Mva1269I GAATGC 1 cut(s) 1120
MvaI CCWGG 5 cut(s) 107, 163, 837, 1307, 1722
MwoI GCNNNNNNNGC 4 cut(s) 676, 873, 1564, 1898
NaeI GCCGGC 1 cut(s) 904
NciI CCSGG 4 cut(s) 553, 891, 892, 1732
NcoI CCATGG 2 cut(s) 1842, 1917
NdeII GATC 5 cut(s) 682, 797, 1054, 1366, 1572
NgoMIV GCCGGC 1 cut(s) 902
NlaIV GGNNCC 6 cut(s) 409, 561, 708, 852, 1629, 2005
NmeAIII GCCGAG 1 cut(s) 1393
NmuCI GTSAC 1 cut(s) 509
NsiI ATGCAT 1 cut(s) 1681
NspI RCATGY 1 cut(s) 700
NspV TTCGAA 1 cut(s) 11
PaeR7I CTCGAG 1 cut(s) 1443
PagI TCATGA 1 cut(s) 1363
PceI AGGCCT 1 cut(s) 818
PciI ACATGT 1 cut(s) 696
PciSI GCTCTTC 1 cut(s) 442
PctI GAATGC 1 cut(s) 1120
PdiI GCCGGC 1 cut(s) 904
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 3 cut(s) 712, 1267, 1285
PfoI TCCNGGA 1 cut(s) 161
PinAI ACCGGT 2 cut(s) 1466, 1894
PkrI GCNGC 2 cut(s) 590, 1040
PleI GAGTC 5 cut(s) 85, 192, 894, 1147, 1845
PpsI GAGTC 5 cut(s) 85, 192, 894, 1147, 1845
PscI ACATGT 1 cut(s) 696
Psp124BI GAGCTC 1 cut(s) 1235
Psp1406I AACGTT 1 cut(s) 16
Psp6I CCWGG 5 cut(s) 105, 161, 835, 1305, 1720
PspGI CCWGG 5 cut(s) 105, 161, 835, 1305, 1720
PspN4I GGNNCC 6 cut(s) 409, 561, 708, 852, 1629, 2005
PspPI GGNCC 4 cut(s) 261, 408, 707, 1911
PspXI VCTCGAGB 1 cut(s) 1443
PstI CTGCAG 1 cut(s) 91
PstNI CAGNNNCTG 2 cut(s) 1586, 1867
RsaI GTAC 5 cut(s) 383, 431, 1821, 1976, 2005
RsaNI GTAC 5 cut(s) 382, 430, 1820, 1975, 2004
SacI GAGCTC 1 cut(s) 1235
SapI GCTCTTC 1 cut(s) 442
SaqAI TTAA 4 cut(s) 59, 387, 672, 734
SatI GCNGC 2 cut(s) 589, 1039
Sau3AI GATC 5 cut(s) 682, 797, 1054, 1366, 1572
Sau96I GGNCC 4 cut(s) 261, 408, 707, 1911
SchI GAGTC 5 cut(s) 86, 192, 895, 1147, 1846
ScrFI CCNGG 9 cut(s) 107, 163, 553, 837, 891, 892, 1307, 1722, 1732
SduI GDGCHC 4 cut(s) 853, 1235, 1414, 1894
SfaNI GCATC 1 cut(s) 1910
SfcI CTRYAG 3 cut(s) 87, 1533, 1578
Sfr274I CTCGAG 1 cut(s) 1443
SfuI TTCGAA 1 cut(s) 11
SinI GGWCC 3 cut(s) 261, 408, 707
SlaI CTCGAG 1 cut(s) 1443
SmaI CCCGGG 1 cut(s) 892
SmlI CTYRAG 1 cut(s) 1443
SmoI CTYRAG 1 cut(s) 1443
SseBI AGGCCT 1 cut(s) 818
SsiI CCGC 7 cut(s) 321, 570, 1038, 1041, 1083, 1289, 1601
SspMI CTAG 5 cut(s) 585, 1451, 1517, 1539, 1662
SstI GAGCTC 1 cut(s) 1235
StuI AGGCCT 1 cut(s) 818
StyD4I CCNGG 9 cut(s) 105, 161, 551, 835, 889, 890, 1305, 1720, 1730
StyI CCWWGG 3 cut(s) 1842, 1917, 1955
TaaI ACNGT 5 cut(s) 412, 934, 1498, 1582, 1619
TaiI ACGT 2 cut(s) 19, 1100
TaqI TCGA 7 cut(s) 11, 96, 494, 741, 1444, 1763, 1809
TatI WGTACW 1 cut(s) 1974
TauI GCSGC 1 cut(s) 1041
TfiI GAWTC 3 cut(s) 712, 1267, 1285
Tru1I TTAA 4 cut(s) 59, 387, 672, 734
Tru9I TTAA 4 cut(s) 59, 387, 672, 734
TscAI CASTG 6 cut(s) 79, 132, 409, 874, 1437, 1803
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 1 cut(s) 588
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 9 cut(s) 524, 936, 1232, 1352, 1392, 1451, 1605, 1661, 1938
TspMI CCCGGG 1 cut(s) 890
TspRI CASTG 6 cut(s) 79, 132, 409, 874, 1437, 1803
VneI GTGCAC 1 cut(s) 1890
VpaK11BI GGWCC 3 cut(s) 261, 408, 707
XapI RAATTY 9 cut(s) 466, 541, 595, 637, 647, 1279, 1686, 1701, 1745
XceI RCATGY 1 cut(s) 700
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XhoI CTCGAG 1 cut(s) 1443
XmaI CCCGGG 1 cut(s) 890
XmiI GTMKAC 1 cut(s) 1967
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 5 cut(s) 585, 1451, 1517, 1539, 1662
Zsp2I ATGCAT 1 cut(s) 1681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.