MD10G1169200.v1.1

Kinase-like protein TMKL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015402
Physical Location & Seq
Reverse (-)
26131615 .. 26134718
3104 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1169200.v1.1.491

Sequence Viewer

Length: 2028 bp
ATGGAGGTTTCGAAACGTTTCTCTCTCTACATTTTCCTCCTCTGCTTCCTCCTACCCTTGAACAGCAAACCCACTGACTCTGTTTCTGCAGATGTCGAGCTCCTCCTGGGAAAAATCAAAGCTTCACTGCAAGGTAACTCTCAGAACTTGCTCTTATCTTCCTGGAACTCCTCTGTGCCTCTCTGCCAATGGAGGGGACTCAAATGGGTTTTCTCCAACGGCTCTATTTTGCTCTGCACCGACCTATCCTCCCCTCAGTGGACCAACTTGTCCCTCTCCAGAGACCCATCTCTCCACCTTTTTTCCCTTCAGCTCCCATCTGCCAATCTCTCTGGTTCTCTTCCCAGAGAGCTGGGGGAGTTCTCTATGCTCCAAAGCTTGTACCTTAACATCAATTTCCTCACTGGGTCCGTCCCATTGGAGCTTGGGTACAGCTCTTCCCTCTCTGACATCGATTTGGGAAGCAATTTGTTGAATGGGGTTTTACCGCCGTCGATCTGGAACTTGTGTGACAATCTGGTTTCCCTTAGGGTTAATGCAAATTCACTATTCGGTTCGGTTCCTGAACCTGCAGTGCGAAACTCTAGCTGCAAGAATTTGCAGTTTCTTGATTTTGGTGGGAACAAGTTTTCTGGGAATTTCCCAGAATTTGTAACCCAGTTTGGTGGGCTTAAAGAGCTTGATCTTGGAAGTAACATGTTTTCGGGTCCGATTCCTGGGGGTTTAGGGCAGTTAAACCTTGAAAAATTGAACCTTTCGCATAATAATTTTAGTGGGGTGTTGCCTGTTTTTGGTGGATCAAAGTTTGGTGTGGAGGCTTTTGAGGGTAACAGCCCTGGACTTTGTGGTGCACCTTTGAAAAGTTGCAGTGGAAACTCTGGGTTGAGTCCAGGGGCAATTGCCGGCATTGTTATTGGTTTGATGACTGCAACTGTGGTTTTGGCTTCATTATGTATTGGGTATGTGCAAAACAAAAAGAAAAACAGCACGGAAGAGAGTGAAGATGAAATAGAGGAAGAAGAAGAAGATGAGGAAAGTGGGAGCGGAGGCGGTGGCGGTGGTGATCAAGGAAGGCTTATTTTGTTTCAGGGCGGCGAGCATTTGACGTTGGAGGATGTGTTGAATGCAACAGGACAAGTTTTGGAGAAGACCAGCTATGGGACTGTGTACAAGGCAAAGCTCTCAGATGGAGGGACCATCACTTTGAGGCTGTTGAAGGAAGGTAGTTGCAAGGATGGGAGCTCTTGTCTTCCTGTGGTGAAGCAACTGGGGAGGATTCGCCATGAGAATTTGATTCCGTTGAGAGCTTTCTATCAGGGGAAGAGAGGGGAGAAGCTTCTGATATATGACCATCTTCCTCACAGAAGCCTTCATGATCTTTTGCATGAAGCCAGAGCAGGGAAACTTGTGTTGAACTGGGCTCGACGACACAAGATTGCACTGGGAATAGCTCGAGGACTAGCATATCTTCATACTGGTCTTGAAACACCTATCACTCATGGGAATGTAAAATCTAAGAATGTGCTAGTGGATGAGTTTTCTGTAGCTAGGCTCACAGAATTTGGGCTTGACAAGCTAATGATCCCTACCGTGGCTGATGAAATGGTGGCGGTTGCAAAAATTGACGGTATTAAGGCACCAGAGCTTCAAAAGATGAAGAAATGTAGTTCAAGGACAGATGTTTATGCATTTGGAATTCTTCTGTTGGAAATTTTGATAGGCAAGAAGCCTGGTAAAACCGGGAGGAGTGGTGATTTTGTGGATTTGCCTTTGATGGTGAAAGTTGCAGTCTTGGAGGAGACGACACTGGAGGTTTTCGACGTGGAGGTACTGAAGGGGGTGAGGAGTGCAATGGAAGAAGGTCTGGTTCAGGCATTGAAACTCGCAATGGGTTGCTGTGCGCCGGTTGCTTCGGTTAGACCCTCCATGGATGAAGTTGTGAGGCAGTTGGAGGAGAACAGGCCAAGGAATAGGTCTACTTTGTACAGCCCAACAGAGACAAGGAGTGAGATTGGTACCCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

676

Amino Acids

72.96

Weight (kDa)

5.98

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 378 - 650 1.2e-24 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 385 - 648 4e-20 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24660
fragaria_vesca FvH4_2g19550
malus_domestica MD05G1179600.v1.1 MD10G1169200.v1.1
prunus_persica Prupe.8G206900_v2.0.a1
pyrus_communis pycom05g16610 pycom10g14600
rosa_chinensis RchiOBHm_Chr6g0285321
rosa_laevigata RLG00000012654
rosa_multiflora Rmu_sc0011958.1_g000002
rosa_roxburghii Rroxscaffold_7G00183360
rosa_rugosa Rorug06G0173500
rosa_samantha Rh6BG285000 Rh6CG286500 Rh6DG279500
rosa_wichuraiana Rw6G024460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 268
Acc36I ACCTGC 1 cut(s) 577
Acc65I GGTACC 1 cut(s) 2015
AccB1I GGYRCC 2 cut(s) 1636, 2015
AccBSI CCGCTC 1 cut(s) 1044
AccI GTMKAC 1 cut(s) 1976
AciI CCGC 6 cut(s) 488, 1044, 1050, 1056, 1092, 1610
AclI AACGTT 1 cut(s) 16
AclWI GGATC 2 cut(s) 805, 1576
AcsI RAATTY 8 cut(s) 541, 595, 637, 647, 1288, 1559, 1695, 1710
AcuI CTGAAG 2 cut(s) 293, 1853
AfaI GTAC 6 cut(s) 383, 431, 1169, 1830, 1985, 2017
AfiI CCNNNNNNNGG 7 cut(s) 193, 258, 716, 791, 1158, 1398, 1591
AflIII ACRYGT 1 cut(s) 696
AjiI CACGTC 1 cut(s) 1822
AjnI CCWGG 6 cut(s) 105, 161, 715, 835, 889, 1729
Alw21I GWGCWC 3 cut(s) 102, 853, 1244
Alw26I GTCTC 3 cut(s) 276, 1793, 1991
Alw44I GTGCAC 1 cut(s) 849
AlwI GGATC 2 cut(s) 805, 1576
Ama87I CYCGRG 1 cut(s) 1452
AoxI GGCC 1 cut(s) 1961
ApaLI GTGCAC 1 cut(s) 849
ApeKI GCWGC 1 cut(s) 588
ApoI RAATTY 8 cut(s) 541, 595, 637, 647, 1288, 1559, 1695, 1710
ArsI GACNNNNNNTTYG 2 cut(s) 1186, 1218
Asp700I GAANNNNTTC 1 cut(s) 17
Asp718I GGTACC 1 cut(s) 2015
AspLEI GCGC 1 cut(s) 1903
AspS9I GGNCC 4 cut(s) 261, 408, 707, 1194
AsuC2I CCSGG 1 cut(s) 1741
AsuHPI GGTGA 5 cut(s) 1073, 1270, 1763, 1789, 1852
AsuII TTCGAA 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 1452
AvaII GGWCC 4 cut(s) 261, 408, 707, 1194
AxyI CCTNAGG 1 cut(s) 527
BaeGI GKGCMC 1 cut(s) 853
BanI GGYRCC 2 cut(s) 1636, 2015
BanII GRGCYC 3 cut(s) 102, 1244, 1423
BbsI GAAGAC 2 cut(s) 1154, 1241
Bbv12I GWGCWC 3 cut(s) 102, 853, 1244
BbvI GCAGC 1 cut(s) 575
BccI CCATC 7 cut(s) 295, 325, 1181, 1205, 1229, 1359, 1768
BceAI ACGGC 2 cut(s) 235, 475
BciT130I CCWGG 6 cut(s) 107, 163, 717, 837, 891, 1731
BclI TGATCA 1 cut(s) 1063
BcnI CCSGG 1 cut(s) 1741
BcoDI GTCTC 3 cut(s) 276, 1793, 1991
BfaI CTAG 4 cut(s) 585, 1460, 1526, 1548
BfmI CTRYAG 3 cut(s) 87, 570, 1542
BfuAI ACCTGC 1 cut(s) 577
BisI GCNGC 2 cut(s) 589, 1093
BlsI GCNGC 2 cut(s) 590, 1094
Bme1390I CCNGG 7 cut(s) 107, 163, 717, 837, 891, 1731, 1741
Bme18I GGWCC 4 cut(s) 261, 408, 707, 1194
BmeT110I CYCGRG 1 cut(s) 1452
BmgBI CACGTC 1 cut(s) 1822
BmgT120I GGNCC 4 cut(s) 261, 408, 707, 1194
BmiI GGNNCC 6 cut(s) 409, 561, 708, 1195, 1638, 2017
BmrFI CCNGG 7 cut(s) 107, 163, 717, 837, 891, 1731, 1741
BmrI ACTGGG 5 cut(s) 414, 652, 1277, 1426, 1451
BmuI ACTGGG 5 cut(s) 414, 652, 1277, 1426, 1451
BpiI GAAGAC 2 cut(s) 1154, 1241
BpmI CTGGAG 2 cut(s) 262, 1829
Bpu14I TTCGAA 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 1741
Bsa29I ATCGAT 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 7 cut(s) 106, 716, 835, 890, 1590, 1926, 1964
Bsc4I CCNNNNNNNGG 7 cut(s) 193, 258, 716, 791, 1158, 1398, 1591
Bse118I RCCGGY 2 cut(s) 902, 1903
Bse1I ACTGG 7 cut(s) 409, 658, 1272, 1421, 1446, 1480, 1812
Bse21I CCTNAGG 1 cut(s) 527
Bse3DI GCAATG 2 cut(s) 1857, 1893
BseBI CCWGG 6 cut(s) 107, 163, 717, 837, 891, 1731
BseCI ATCGAT 1 cut(s) 453
BseDI CCNNGG 7 cut(s) 106, 716, 835, 890, 1590, 1926, 1964
BseGI GGATG 4 cut(s) 1120, 1240, 1537, 1936
BseLI CCNNNNNNNGG 7 cut(s) 193, 258, 716, 791, 1158, 1398, 1591
BseMI GCAATG 2 cut(s) 1857, 1893
BseMII CTCAG 3 cut(s) 155, 269, 1197
BseNI ACTGG 7 cut(s) 409, 658, 1272, 1421, 1446, 1480, 1812
BseRI GAGGAG 7 cut(s) 29, 92, 160, 1759, 1811, 1858, 1967
BseSI GKGCMC 1 cut(s) 853
BseXI GCAGC 1 cut(s) 575
BseYI CCCAGC 1 cut(s) 352
BsgI GTGCAG 1 cut(s) 220
BshFI GGCC 1 cut(s) 1963
BshNI GGYRCC 2 cut(s) 1636, 2015
BshVI ATCGAT 1 cut(s) 453
BsiHKAI GWGCWC 3 cut(s) 102, 853, 1244
BsiHKCI CYCGRG 1 cut(s) 1452
BsiSI CCGG 3 cut(s) 903, 1740, 1904
BslFI GGGAC 5 cut(s) 210, 256, 398, 1174, 1207
BslI CCNNNNNNNGG 7 cut(s) 193, 258, 716, 791, 1158, 1398, 1591
BsmAI GTCTC 3 cut(s) 276, 1793, 1991
BsmBI CGTCTC 1 cut(s) 1793
BsmFI GGGAC 5 cut(s) 210, 256, 398, 1174, 1207
BsmI GAATGC 1 cut(s) 1129
BsnI GGCC 1 cut(s) 1963
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 1452
Bsp119I TTCGAA 1 cut(s) 11
Bsp1286I GDGCHC 4 cut(s) 102, 853, 1244, 1423
Bsp1407I TGTACA 2 cut(s) 1167, 1983
Bsp143I GATC 6 cut(s) 495, 682, 797, 1063, 1375, 1581
Bsp19I CCATGG 1 cut(s) 1926
BspACI CCGC 6 cut(s) 488, 1044, 1050, 1056, 1092, 1610
BspANI GGCC 1 cut(s) 1963
BspCNI CTCAG 3 cut(s) 154, 268, 1196
BspDI ATCGAT 1 cut(s) 453
BspHI TCATGA 1 cut(s) 1372
BspLI GGNNCC 6 cut(s) 409, 561, 708, 1195, 1638, 2017
BspMAI CTGCAG 2 cut(s) 91, 574
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 2 cut(s) 805, 1576
BspQI GCTCTTC 1 cut(s) 442
BspT104I TTCGAA 1 cut(s) 11
BspT107I GGYRCC 2 cut(s) 1636, 2015
BspTNI GGTCTC 1 cut(s) 276
BsrBI CCGCTC 1 cut(s) 1044
BsrDI GCAATG 2 cut(s) 1857, 1893
BsrFI RCCGGY 2 cut(s) 902, 1903
BsrGI TGTACA 2 cut(s) 1167, 1983
BsrI ACTGG 7 cut(s) 409, 658, 1272, 1421, 1446, 1480, 1812
BssAI RCCGGY 2 cut(s) 902, 1903
BssECI CCNNGG 7 cut(s) 106, 716, 835, 890, 1590, 1926, 1964
BssMI GATC 6 cut(s) 495, 682, 797, 1063, 1375, 1581
BssT1I CCWWGG 2 cut(s) 1926, 1964
Bst2UI CCWGG 6 cut(s) 107, 163, 717, 837, 891, 1731
Bst4CI ACNGT 4 cut(s) 934, 1165, 1591, 1628
Bst6I CTCTTC 4 cut(s) 345, 442, 987, 1316
BstAUI TGTACA 2 cut(s) 1167, 1983
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 904, 1097
BstDEI CTNAG 5 cut(s) 141, 255, 527, 1183, 1515
BstDSI CCRYGG 2 cut(s) 1590, 1926
BstF5I GGATG 4 cut(s) 1120, 1240, 1537, 1936
BstHHI GCGC 1 cut(s) 1903
BstKTI GATC 6 cut(s) 498, 685, 800, 1066, 1378, 1584
BstMAI GTCTC 3 cut(s) 276, 1793, 1991
BstMBI GATC 6 cut(s) 495, 682, 797, 1063, 1375, 1581
BstMWI GCNNNNNNNGC 3 cut(s) 676, 1573, 1907
BstNI CCWGG 6 cut(s) 107, 163, 717, 837, 891, 1731
BstNSI RCATGY 1 cut(s) 700
BstSCI CCNGG 7 cut(s) 105, 161, 715, 835, 889, 1729, 1739
BstSFI CTRYAG 3 cut(s) 87, 570, 1542
BstSLI GKGCMC 1 cut(s) 853
BstV1I GCAGC 1 cut(s) 575
BstV2I GAAGAC 2 cut(s) 1154, 1241
BstXI CCANNNNNNTGG 2 cut(s) 352, 665
Bsu15I ATCGAT 1 cut(s) 453
Bsu36I CCTNAGG 1 cut(s) 527
BsuRI GGCC 1 cut(s) 1963
BsuTUI ATCGAT 1 cut(s) 453
BtgI CCRYGG 2 cut(s) 1590, 1926
BtrI CACGTC 1 cut(s) 1822
BtsCI GGATG 4 cut(s) 1120, 1240, 1537, 1936
BtsI GCAGTG 3 cut(s) 125, 579, 874
BtsIMutI CAGTG 8 cut(s) 72, 125, 263, 402, 579, 874, 1439, 1805
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 2 cut(s) 904, 1097
CciI TCATGA 1 cut(s) 1372
CfoI GCGC 1 cut(s) 1903
Cfr10I RCCGGY 2 cut(s) 902, 1903
Cfr13I GGNCC 4 cut(s) 261, 408, 707, 1194
ClaI ATCGAT 1 cut(s) 453
Csp6I GTAC 6 cut(s) 382, 430, 1168, 1829, 1984, 2016
CviAII CATG 6 cut(s) 697, 1283, 1373, 1385, 1499, 1927
CviQI GTAC 6 cut(s) 382, 430, 1168, 1829, 1984, 2016
DdeI CTNAG 5 cut(s) 141, 255, 527, 1183, 1515
DpnI GATC 6 cut(s) 497, 684, 799, 1065, 1377, 1583
DpnII GATC 6 cut(s) 495, 682, 797, 1063, 1375, 1581
DrdI GACNNNNNNGTC 1 cut(s) 268
DseDI GACNNNNNNGTC 1 cut(s) 268
Eam1104I CTCTTC 4 cut(s) 345, 442, 987, 1316
EarI CTCTTC 4 cut(s) 345, 442, 987, 1316
Ecl136II GAGCTC 2 cut(s) 100, 1242
Eco130I CCWWGG 2 cut(s) 1926, 1964
Eco24I GRGCYC 3 cut(s) 102, 1244, 1423
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 4 cut(s) 261, 408, 707, 1194
Eco53kI GAGCTC 2 cut(s) 100, 1242
Eco57I CTGAAG 2 cut(s) 293, 1853
Eco81I CCTNAGG 1 cut(s) 527
Eco88I CYCGRG 1 cut(s) 1452
EcoICRI GAGCTC 2 cut(s) 100, 1242
EcoRI GAATTC 1 cut(s) 1695
EcoRII CCWGG 6 cut(s) 105, 161, 715, 835, 889, 1729
EcoT14I CCWWGG 2 cut(s) 1926, 1964
EcoT22I ATGCAT 1 cut(s) 1690
EcoT38I GRGCYC 3 cut(s) 102, 1244, 1423
ErhI CCWWGG 2 cut(s) 1926, 1964
Esp3I CGTCTC 1 cut(s) 1793
FaeI CATG 6 cut(s) 700, 1286, 1376, 1388, 1502, 1930
FalI AAGNNNNNCTT 2 cut(s) 1059, 1091
FaqI GGGAC 5 cut(s) 210, 256, 398, 1174, 1207
FatI CATG 6 cut(s) 696, 1282, 1372, 1384, 1498, 1926
FbaI TGATCA 1 cut(s) 1063
FblI GTMKAC 1 cut(s) 1976
Fnu4HI GCNGC 2 cut(s) 589, 1093
FokI GGATG 4 cut(s) 1127, 1247, 1544, 1943
FriOI GRGCYC 3 cut(s) 102, 1244, 1423
Fsp4HI GCNGC 2 cut(s) 589, 1093
FspBI CTAG 4 cut(s) 585, 1460, 1526, 1548
GlaI GCGC 1 cut(s) 1902
GluI GCNGC 2 cut(s) 589, 1093
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 2 cut(s) 262, 1829
HaeIII GGCC 1 cut(s) 1963
HapII CCGG 3 cut(s) 903, 1740, 1904
HhaI GCGC 1 cut(s) 1903
Hin1II CATG 6 cut(s) 700, 1286, 1376, 1388, 1502, 1930
Hin6I GCGC 1 cut(s) 1901
HinP1I GCGC 1 cut(s) 1901
HindIII AAGCTT 3 cut(s) 120, 376, 1334
HinfI GANTC 6 cut(s) 77, 198, 712, 886, 1276, 1294
HpaII CCGG 3 cut(s) 903, 1740, 1904
HphI GGTGA 5 cut(s) 1073, 1270, 1763, 1789, 1852
Hpy166II GTNNAC 4 cut(s) 261, 851, 1168, 1977
Hpy188I TCNGA 5 cut(s) 144, 448, 711, 1186, 1341
Hpy188III TCNNGA 6 cut(s) 279, 499, 563, 608, 1373, 1481
Hpy8I GTNNAC 4 cut(s) 261, 851, 1168, 1977
Hpy99I CGWCG 3 cut(s) 496, 1428, 1823
HpyAV CCTTC 7 cut(s) 317, 1065, 1210, 1214, 1379, 1828, 1853
HpyCH4III ACNGT 4 cut(s) 934, 1165, 1591, 1628
HpyCH4IV ACGT 3 cut(s) 16, 1106, 1821
HpyF10VI GCNNNNNNNGC 3 cut(s) 676, 1573, 1907
HpyF3I CTNAG 5 cut(s) 141, 255, 527, 1183, 1515
HpySE526I ACGT 3 cut(s) 16, 1106, 1821
Hsp92II CATG 6 cut(s) 700, 1286, 1376, 1388, 1502, 1930
HspAI GCGC 1 cut(s) 1901
KpnI GGTACC 1 cut(s) 2019
KroI GCCGGC 1 cut(s) 902
KroNI GCCGGC 1 cut(s) 904
Ksp22I TGATCA 1 cut(s) 1063
Kzo9I GATC 6 cut(s) 495, 682, 797, 1063, 1375, 1581
LguI GCTCTTC 1 cut(s) 442
LmnI GCTCC 6 cut(s) 105, 318, 375, 421, 1041, 1239
Lsp1109I GCAGC 1 cut(s) 575
MaeI CTAG 4 cut(s) 585, 1460, 1526, 1548
MaeII ACGT 3 cut(s) 16, 1106, 1821
MaeIII GTNAC 5 cut(s) 134, 509, 652, 692, 827
MalI GATC 6 cut(s) 497, 684, 799, 1065, 1377, 1583
MbiI CCGCTC 1 cut(s) 1044
MboI GATC 6 cut(s) 495, 682, 797, 1063, 1375, 1581
MfeI CAATTG 1 cut(s) 897
MhlI GDGCHC 4 cut(s) 102, 853, 1244, 1423
MlyI GAGTC 3 cut(s) 71, 192, 895
MmeI TCCRAC 4 cut(s) 240, 1089, 1686, 1929
Mph1103I ATGCAT 1 cut(s) 1690
MroNI GCCGGC 1 cut(s) 902
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 5 cut(s) 387, 534, 672, 734, 1632
MslI CAYNNNNRTG 1 cut(s) 1503
MspI CCGG 3 cut(s) 903, 1740, 1904
MspR9I CCNGG 7 cut(s) 107, 163, 717, 837, 891, 1731, 1741
MunI CAATTG 1 cut(s) 897
Mva1269I GAATGC 1 cut(s) 1129
MvaI CCWGG 6 cut(s) 107, 163, 717, 837, 891, 1731
MwoI GCNNNNNNNGC 3 cut(s) 676, 1573, 1907
NaeI GCCGGC 1 cut(s) 904
NciI CCSGG 1 cut(s) 1741
NcoI CCATGG 1 cut(s) 1926
NdeII GATC 6 cut(s) 495, 682, 797, 1063, 1375, 1581
NgoMIV GCCGGC 1 cut(s) 902
NlaIII CATG 6 cut(s) 700, 1286, 1376, 1388, 1502, 1930
NlaIV GGNNCC 6 cut(s) 409, 561, 708, 1195, 1638, 2017
NmuCI GTSAC 1 cut(s) 509
NsiI ATGCAT 1 cut(s) 1690
NspI RCATGY 1 cut(s) 700
NspV TTCGAA 1 cut(s) 11
PaeR7I CTCGAG 1 cut(s) 1452
PagI TCATGA 1 cut(s) 1372
PciI ACATGT 1 cut(s) 696
PciSI GCTCTTC 1 cut(s) 442
PctI GAATGC 1 cut(s) 1129
PdiI GCCGGC 1 cut(s) 904
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 3 cut(s) 712, 1276, 1294
PfoI TCCNGGA 1 cut(s) 161
PkrI GCNGC 2 cut(s) 590, 1094
PleI GAGTC 3 cut(s) 71, 192, 894
PpsI GAGTC 3 cut(s) 71, 192, 894
PscI ACATGT 1 cut(s) 696
Psp124BI GAGCTC 2 cut(s) 102, 1244
Psp1406I AACGTT 1 cut(s) 16
Psp6I CCWGG 6 cut(s) 105, 161, 715, 835, 889, 1729
PspFI CCCAGC 1 cut(s) 352
PspGI CCWGG 6 cut(s) 105, 161, 715, 835, 889, 1729
PspN4I GGNNCC 6 cut(s) 409, 561, 708, 1195, 1638, 2017
PspPI GGNCC 4 cut(s) 261, 408, 707, 1194
PspXI VCTCGAGB 1 cut(s) 1452
PstI CTGCAG 2 cut(s) 91, 574
RsaI GTAC 6 cut(s) 383, 431, 1169, 1830, 1985, 2017
RsaNI GTAC 6 cut(s) 382, 430, 1168, 1829, 1984, 2016
RseI CAYNNNNRTG 1 cut(s) 1503
SacI GAGCTC 2 cut(s) 102, 1244
SapI GCTCTTC 1 cut(s) 442
SaqAI TTAA 5 cut(s) 387, 534, 672, 734, 1632
SatI GCNGC 2 cut(s) 589, 1093
Sau3AI GATC 6 cut(s) 495, 682, 797, 1063, 1375, 1581
Sau96I GGNCC 4 cut(s) 261, 408, 707, 1194
SchI GAGTC 3 cut(s) 71, 192, 895
ScrFI CCNGG 7 cut(s) 107, 163, 717, 837, 891, 1731, 1741
SduI GDGCHC 4 cut(s) 102, 853, 1244, 1423
SfcI CTRYAG 3 cut(s) 87, 570, 1542
Sfr274I CTCGAG 1 cut(s) 1452
SfuI TTCGAA 1 cut(s) 11
SinI GGWCC 4 cut(s) 261, 408, 707, 1194
SlaI CTCGAG 1 cut(s) 1452
SmiMI CAYNNNNRTG 1 cut(s) 1503
SmlI CTYRAG 1 cut(s) 1452
SmoI CTYRAG 1 cut(s) 1452
SsiI CCGC 6 cut(s) 488, 1044, 1050, 1056, 1092, 1610
SspMI CTAG 4 cut(s) 585, 1460, 1526, 1548
SstI GAGCTC 2 cut(s) 102, 1244
StyD4I CCNGG 7 cut(s) 105, 161, 715, 835, 889, 1729, 1739
StyI CCWWGG 2 cut(s) 1926, 1964
TaaI ACNGT 4 cut(s) 934, 1165, 1591, 1628
TaiI ACGT 3 cut(s) 19, 1109, 1824
TaqI TCGA 7 cut(s) 11, 96, 453, 494, 1423, 1453, 1818
TatI WGTACW 2 cut(s) 1167, 1983
TauI GCSGC 1 cut(s) 1095
TfiI GAWTC 3 cut(s) 712, 1276, 1294
Tru1I TTAA 5 cut(s) 387, 534, 672, 734, 1632
Tru9I TTAA 5 cut(s) 387, 534, 672, 734, 1632
TscAI CASTG 8 cut(s) 79, 132, 263, 409, 579, 874, 1446, 1812
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 1 cut(s) 588
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 8 cut(s) 936, 1020, 1361, 1401, 1460, 1614, 1670, 1947
TspGWI ACGGA 3 cut(s) 400, 1004, 1287
TspRI CASTG 8 cut(s) 79, 132, 263, 409, 579, 874, 1446, 1812
VneI GTGCAC 1 cut(s) 849
VpaK11BI GGWCC 4 cut(s) 261, 408, 707, 1194
XapI RAATTY 8 cut(s) 541, 595, 637, 647, 1288, 1559, 1695, 1710
XceI RCATGY 1 cut(s) 700
XhoI CTCGAG 1 cut(s) 1452
XmiI GTMKAC 1 cut(s) 1976
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 4 cut(s) 585, 1460, 1526, 1548
Zsp2I ATGCAT 1 cut(s) 1690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.