RLG00000012654

Kinase-like protein TMKL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015402
Physical Location & Seq
Forward (+)
20943156 .. 20945888
2733 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012654

Sequence Viewer

Length: 2025 bp
ATGGAGATTTTGAAACCCTTGTTTTGCTTTCTGCTCTACTTCATTCTATGCTTCAATGTCTTACCCACTTGGTCTTCTTCTTCTTCTTCAGATGTTGAGCTTCTGTTGGGAAACATCAAGGCTTCACTGCAAGGTAACGCTCAGAACTTATTGTTAACTTCATGGAACACTTCTCTGCCTCTGTGCCAATGGAGAGGCCTGAAATGGGTTTTCTCCAATGGCTCTCCTTTGCTTTGCAATGACATGTCCTCACCCCATTGGACCAATCTTTCCCTCTCCAGAGACCGAAGTCTTCACCTTTACTCTCTTCAGCTCCCCTCTGCTAATCTCTCTGGTTCAATACCCAGAGAGATAGGGGAGTTTTCTATGCTCAGAAGCTTGTACCTTAGTGTTAATTCACTGAGTGGGACCATCCCACTTGAGCTTGGCTACAGCTCTTCACTTACTGAAATTGATTTGGGCAACAATTTGTTCACTGGGGCTTTAGCTCCATCTGTGTGGAACTTGTGTGAGAATCTTGTCTCGCTTAGGCTTCATGGTAATTCGCTATCTGGGCCTGTTCCTGAACCTGCATTGCCCAATTCTACCTGCAAAAATTTGCAGCTACTGGATTTGGGTAACAACAAGTTTTCAGGGAACTTCCCTGAGTTTGTGAGCCAGTTTGGTGGGCTGAAGGAGCTTGATCTTGGGAAAAACATGATTTCGGGTCCAGTTCCGGTGAGTTTAGCTCAGCTGGAACTTGACAAGTTGAACCTTTCACACAATAACTTTAGTGGGGTGCTGCCAGCTTTTGGTGGATCAAAGTTTGGTGTGGAGTCTTTTGAGGGGAACAACCCTGGACTTTGTGGGGCGCCCTTGAGAAGTTGTAGTGGAAGCTCTGGGTTGAGCCCTGGAGCAATTGCTGGTATTGTCATTGGTTTGATGGCTGGGACTGTGGTTTTGGCTTCACTGTGTATTGGGTATGTGCAACACAAGAAGAAAAACAGCAGCGCAGAGAGTGACTACGAGTTGGAGGAAGGAGAAGAAGAAGAAGAAAATGGGGGTCTTGGTGATGGCGGTGGTGATCAGGGAAGGCTTATATTGTTCCAGGGAGGTGAGCATTTGACATTAGACGATGTGTTGAATGCAACAGGACAAGTGTTGGAGAAGACCAGCTATGGGACTGTTTATAAGGCAAAGCTTGCTGATGGGGGCACCATTACTTTAAGGTTGTTGAGGGAAGGTAGTTGCAAAGATGGGAGCTCTTGTGTGCCTGTAATAAAGCAATTGGGCCGCATTCGCCATGAGAATTTGATTCCATTGAGAGCTTTCTATCAGGGGAAGCGAGGGGAGAAGCTTCTGATATATGACCATCTTCCTCTCAAAAGCCTTCATGATCTTTTACATGAATCTAGAGTAGGGAAACCTGTGCTGAACTGGGCCCGGCGACACAAAATTGCCTTGGGTATAGCTAGAGGACTAGCATGCCTCCATGCTCTTGAAACGCCTGTTACTCATGGGACTGTAAGATCTAAGAATGTGCTTGTGGATGAGTTTTTTGTATCTAGGCTCACTGAGTTCGGGCTTGACAAGGTGATGATCCCCTCTGTAGCCGATGAAATGGTGGCTGTTGCAAAGGCTGATGGTTACAAGGCACCGGAGCTTCAAAAGATGAAGAAATGCAATTCTCGGACGGATGTTTATGCATTTGGGATTTTGCTATTGGAAATTCTGATAGGCAAGAAGCCTGGTAAAAGCGGGAGGAGTGGCGAATTTGTGGATTTGCCTTCACTGGTCAAAGTGGCAGTTCTGGAGGAGACAACATTGGAGGTTTTTGATGTGGAGGTATTGAAGGGGATAAGGAATCCCATGGAAGAAGGTTTGGTTCAGGCATTGAAACTTGCAATGGGTTGCTGTGCTCCAGTTGCTTCAGTTAGACCCTCCATGGATGAAGTTGTGAAGCAGTTGGAGGAGAACAGACCAAGGAATAGGTCTGCTTTGTACAGCCCAGCAGAAGAAAGGAGTGAGATTGGTACCCCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

675

Amino Acids

72.98

Weight (kDa)

6.07

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 199 - 257 1.5e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 378 - 649 2.3e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 385 - 647 7e-19 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24660
fragaria_vesca FvH4_2g19550
malus_domestica MD05G1179600.v1.1 MD10G1169200.v1.1
prunus_persica Prupe.8G206900_v2.0.a1
pyrus_communis pycom05g16610 pycom10g14600
rosa_chinensis RchiOBHm_Chr6g0285321
rosa_laevigata RLG00000012654
rosa_multiflora Rmu_sc0011958.1_g000002
rosa_roxburghii Rroxscaffold_7G00183360
rosa_rugosa Rorug06G0173500
rosa_samantha Rh6BG285000 Rh6CG286500 Rh6DG279500
rosa_wichuraiana Rw6G024460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1170
Acc36I ACCTGC 2 cut(s) 577, 596
Acc65I GGTACC 1 cut(s) 2012
AccB1I GGYRCC 4 cut(s) 850, 1193, 1633, 2012
AccB7I CCANNNNNTGG 1 cut(s) 791
AciI CCGC 3 cut(s) 1056, 1273, 1737
AclWI GGATC 2 cut(s) 805, 1573
AcsI RAATTY 4 cut(s) 595, 1288, 1707, 1751
AcuI CTGAAG 4 cut(s) 72, 293, 692, 1893
AcyI GRCGYC 1 cut(s) 851
AdeI CACNNNGTG 1 cut(s) 404
AfaI GTAC 3 cut(s) 383, 1982, 2014
AfiI CCNNNNNNNGG 3 cut(s) 205, 791, 1158
AflIII ACRYGT 1 cut(s) 243
AgsI TTSAA 9 cut(s) 13, 55, 339, 751, 1123, 1481, 1646, 1831, 1876
AjnI CCWGG 4 cut(s) 835, 889, 1086, 1726
Alw21I GWGCWC 2 cut(s) 1244, 1900
Alw26I GTCTC 3 cut(s) 276, 526, 1790
AlwI GGATC 2 cut(s) 805, 1573
AlwNI CAGNNNCTG 1 cut(s) 607
AoxI GGCC 4 cut(s) 196, 554, 1270, 1419
ApaI GGGCCC 1 cut(s) 1423
ApeKI GCWGC 3 cut(s) 601, 781, 987
ApoI RAATTY 4 cut(s) 595, 1288, 1707, 1751
ArsI GACNNNNNNTTYG 2 cut(s) 922, 954
Asp718I GGTACC 1 cut(s) 2012
AspLEI GCGC 2 cut(s) 853, 992
AspS9I GGNCC 7 cut(s) 261, 408, 554, 707, 1270, 1419, 1420
AsuC2I CCSGG 1 cut(s) 1423
AsuHPI GGTGA 7 cut(s) 243, 287, 730, 1061, 1073, 1106, 1585
AvaII GGWCC 3 cut(s) 261, 408, 707
BaeGI GKGCMC 2 cut(s) 1196, 1423
BanI GGYRCC 4 cut(s) 850, 1193, 1633, 2012
BanII GRGCYC 3 cut(s) 890, 1244, 1423
BbsI GAAGAC 3 cut(s) 66, 284, 1154
Bbv12I GWGCWC 2 cut(s) 1244, 1900
BbvI GCAGC 3 cut(s) 613, 768, 999
BccI CCATC 8 cut(s) 419, 499, 916, 1046, 1181, 1229, 1359, 1616
BciT130I CCWGG 4 cut(s) 837, 891, 1088, 1728
BclI TGATCA 1 cut(s) 1063
BcnI CCSGG 1 cut(s) 1423
BcoDI GTCTC 3 cut(s) 276, 526, 1790
BfaI CTAG 4 cut(s) 1392, 1452, 1460, 1545
BfmI CTRYAG 2 cut(s) 430, 1587
BfoI RGCGCY 1 cut(s) 854
BfuAI ACCTGC 2 cut(s) 577, 596
BglII AGATCT 1 cut(s) 1508
BisI GCNGC 4 cut(s) 602, 782, 988, 1273
BlpI GCTNAGC 1 cut(s) 729
BlsI GCNGC 4 cut(s) 603, 783, 989, 1274
Bme1390I CCNGG 5 cut(s) 837, 891, 1088, 1423, 1728
Bme18I GGWCC 3 cut(s) 261, 408, 707
BmgT120I GGNCC 7 cut(s) 261, 408, 554, 707, 1270, 1419, 1420
BmiI GGNNCC 7 cut(s) 409, 708, 852, 1195, 1421, 1635, 2014
BmrFI CCNGG 5 cut(s) 837, 891, 1088, 1423, 1728
BmrI ACTGGG 2 cut(s) 486, 1426
BmuI ACTGGG 2 cut(s) 486, 1426
BoxI GACNNNNGTC 1 cut(s) 288
BpiI GAAGAC 3 cut(s) 66, 284, 1154
BplI GAGNNNNNCTC 2 cut(s) 712, 744
BpmI CTGGAG 4 cut(s) 262, 912, 1811, 1884
Bpu10I CCTNAGC 1 cut(s) 527
Bpu1102I GCTNAGC 1 cut(s) 729
BpuEI CTTGAG 2 cut(s) 440, 877
BpuMI CCSGG 1 cut(s) 1423
BsaHI GRCGYC 1 cut(s) 851
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 7 cut(s) 835, 889, 1087, 1440, 1848, 1923, 1961
BsaWI WCCGGW 2 cut(s) 715, 1636
BsaXI ACNNNNNCTCC 2 cut(s) 1231, 1261
Bsc4I CCNNNNNNNGG 3 cut(s) 205, 791, 1158
Bse1I ACTGG 7 cut(s) 481, 612, 658, 710, 1421, 1776, 1901
Bse3DI GCAATG 3 cut(s) 244, 572, 1890
BseBI CCWGG 4 cut(s) 837, 891, 1088, 1728
BseDI CCNNGG 7 cut(s) 835, 889, 1087, 1440, 1848, 1923, 1961
BseGI GGATG 4 cut(s) 411, 1534, 1681, 1933
BseLI CCNNNNNNNGG 3 cut(s) 205, 791, 1158
BseMI GCAATG 3 cut(s) 244, 572, 1890
BseMII CTCAG 6 cut(s) 155, 385, 392, 636, 743, 1545
BseNI ACTGG 7 cut(s) 481, 612, 658, 710, 1421, 1776, 1901
BseRI GAGGAG 3 cut(s) 1756, 1808, 1964
BseSI GKGCMC 2 cut(s) 1196, 1423
BseXI GCAGC 3 cut(s) 613, 768, 999
BseYI CCCAGC 2 cut(s) 926, 1987
BshFI GGCC 4 cut(s) 198, 556, 1272, 1421
BshNI GGYRCC 4 cut(s) 850, 1193, 1633, 2012
BsiHKAI GWGCWC 2 cut(s) 1244, 1900
BsiSI CCGG 3 cut(s) 716, 1423, 1637
BslFI GGGAC 4 cut(s) 421, 943, 1174, 1513
BslI CCNNNNNNNGG 3 cut(s) 205, 791, 1158
BsmAI GTCTC 3 cut(s) 276, 526, 1790
BsmFI GGGAC 4 cut(s) 421, 943, 1174, 1513
BsmI GAATGC 2 cut(s) 1129, 1275
BsnI GGCC 4 cut(s) 198, 556, 1272, 1421
Bso31I GGTCTC 1 cut(s) 276
Bsp120I GGGCCC 1 cut(s) 1419
Bsp1286I GDGCHC 5 cut(s) 890, 1196, 1244, 1423, 1900
Bsp1407I TGTACA 1 cut(s) 1980
Bsp143I GATC 6 cut(s) 682, 797, 1063, 1375, 1508, 1578
Bsp1720I GCTNAGC 1 cut(s) 729
Bsp19I CCATGG 2 cut(s) 1848, 1923
BspACI CCGC 3 cut(s) 1056, 1273, 1737
BspANI GGCC 4 cut(s) 198, 556, 1272, 1421
BspCNI CTCAG 6 cut(s) 154, 384, 393, 637, 742, 1546
BspHI TCATGA 1 cut(s) 1372
BspLI GGNNCC 7 cut(s) 409, 708, 852, 1195, 1421, 1635, 2014
BspMI ACCTGC 2 cut(s) 577, 596
BspPI GGATC 2 cut(s) 805, 1573
BspQI GCTCTTC 1 cut(s) 442
BspT107I GGYRCC 4 cut(s) 850, 1193, 1633, 2012
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 3 cut(s) 244, 572, 1890
BsrGI TGTACA 1 cut(s) 1980
BsrI ACTGG 7 cut(s) 481, 612, 658, 710, 1421, 1776, 1901
BssECI CCNNGG 7 cut(s) 835, 889, 1087, 1440, 1848, 1923, 1961
BssMI GATC 6 cut(s) 682, 797, 1063, 1375, 1508, 1578
BssNI GRCGYC 1 cut(s) 851
BssT1I CCWWGG 4 cut(s) 1440, 1848, 1923, 1961
Bst2UI CCWGG 4 cut(s) 837, 891, 1088, 1728
Bst4CI ACNGT 4 cut(s) 934, 951, 1165, 1504
Bst6I CTCTTC 2 cut(s) 312, 442
BstACI GRCGYC 1 cut(s) 851
BstAPI GCANNNNNTGC 1 cut(s) 1181
BstAUI TGTACA 1 cut(s) 1980
BstC8I GCNNGC 3 cut(s) 786, 1182, 1465
BstDEI CTNAG 9 cut(s) 141, 371, 386, 401, 527, 645, 729, 1512, 1554
BstDSI CCRYGG 2 cut(s) 1848, 1923
BstF5I GGATG 4 cut(s) 411, 1534, 1681, 1933
BstH2I RGCGCY 1 cut(s) 854
BstHHI GCGC 2 cut(s) 853, 992
BstKTI GATC 6 cut(s) 685, 800, 1066, 1378, 1511, 1581
BstMAI GTCTC 3 cut(s) 276, 526, 1790
BstMBI GATC 6 cut(s) 682, 797, 1063, 1375, 1508, 1578
BstMWI GCNNNNNNNGC 5 cut(s) 553, 676, 1181, 1278, 1904
BstNI CCWGG 4 cut(s) 837, 891, 1088, 1728
BstNSI RCATGY 2 cut(s) 247, 1467
BstPAI GACNNNNGTC 1 cut(s) 288
BstSCI CCNGG 5 cut(s) 835, 889, 1086, 1421, 1726
BstSFI CTRYAG 2 cut(s) 430, 1587
BstSLI GKGCMC 2 cut(s) 1196, 1423
BstV1I GCAGC 3 cut(s) 613, 768, 999
BstV2I GAAGAC 3 cut(s) 66, 284, 1154
BstX2I RGATCY 1 cut(s) 1508
BstXI CCANNNNNNTGG 2 cut(s) 498, 665
BstYI RGATCY 1 cut(s) 1508
BsuRI GGCC 4 cut(s) 198, 556, 1272, 1421
BtgI CCRYGG 2 cut(s) 1848, 1923
BtsCI GGATG 4 cut(s) 411, 1534, 1681, 1933
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 6 cut(s) 125, 398, 474, 947, 1551, 1769
BveI ACCTGC 2 cut(s) 577, 596
Cac8I GCNNGC 3 cut(s) 786, 1182, 1465
CaiI CAGNNNCTG 1 cut(s) 607
CciI TCATGA 1 cut(s) 1372
CfoI GCGC 2 cut(s) 853, 992
Cfr13I GGNCC 7 cut(s) 261, 408, 554, 707, 1270, 1419, 1420
Csp6I GTAC 3 cut(s) 382, 1981, 2013
CviQI GTAC 3 cut(s) 382, 1981, 2013
DdeI CTNAG 9 cut(s) 141, 371, 386, 401, 527, 645, 729, 1512, 1554
DinI GGCGCC 1 cut(s) 852
DpnI GATC 6 cut(s) 684, 799, 1065, 1377, 1510, 1580
DpnII GATC 6 cut(s) 682, 797, 1063, 1375, 1508, 1578
DraIII CACNNNGTG 1 cut(s) 404
Eam1104I CTCTTC 2 cut(s) 312, 442
EarI CTCTTC 2 cut(s) 312, 442
Ecl136II GAGCTC 1 cut(s) 1242
Eco130I CCWWGG 4 cut(s) 1440, 1848, 1923, 1961
Eco147I AGGCCT 1 cut(s) 198
Eco24I GRGCYC 3 cut(s) 890, 1244, 1423
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 3 cut(s) 261, 408, 707
Eco53kI GAGCTC 1 cut(s) 1242
Eco57I CTGAAG 4 cut(s) 72, 293, 692, 1893
EcoICRI GAGCTC 1 cut(s) 1242
EcoRII CCWGG 4 cut(s) 835, 889, 1086, 1726
EcoT14I CCWWGG 4 cut(s) 1440, 1848, 1923, 1961
EcoT22I ATGCAT 1 cut(s) 1687
EcoT38I GRGCYC 3 cut(s) 890, 1244, 1423
EgeI GGCGCC 1 cut(s) 852
EheI GGCGCC 1 cut(s) 852
ErhI CCWWGG 4 cut(s) 1440, 1848, 1923, 1961
FaqI GGGAC 4 cut(s) 421, 943, 1174, 1513
FauI CCCGC 1 cut(s) 1730
FbaI TGATCA 1 cut(s) 1063
Fnu4HI GCNGC 4 cut(s) 602, 782, 988, 1273
FokI GGATG 4 cut(s) 398, 1541, 1688, 1940
FriOI GRGCYC 3 cut(s) 890, 1244, 1423
Fsp4HI GCNGC 4 cut(s) 602, 782, 988, 1273
FspBI CTAG 4 cut(s) 1392, 1452, 1460, 1545
GlaI GCGC 2 cut(s) 852, 991
GluI GCNGC 4 cut(s) 602, 782, 988, 1273
GsaI CCCAGC 2 cut(s) 930, 1991
GsuI CTGGAG 4 cut(s) 262, 912, 1811, 1884
HaeII RGCGCY 1 cut(s) 854
HaeIII GGCC 4 cut(s) 198, 556, 1272, 1421
HapII CCGG 3 cut(s) 716, 1423, 1637
HhaI GCGC 2 cut(s) 853, 992
Hin1I GRCGYC 1 cut(s) 851
Hin6I GCGC 2 cut(s) 851, 990
HinP1I GCGC 2 cut(s) 851, 990
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HindIII AAGCTT 3 cut(s) 376, 1178, 1334
HinfI GANTC 5 cut(s) 514, 815, 1294, 1388, 1843
HpaI GTTAAC 1 cut(s) 156
HpaII CCGG 3 cut(s) 716, 1423, 1637
HphI GGTGA 7 cut(s) 243, 287, 730, 1061, 1073, 1106, 1585
Hpy166II GTNNAC 2 cut(s) 156, 474
Hpy188I TCNGA 6 cut(s) 91, 144, 374, 1341, 1671, 1713
Hpy188III TCNNGA 6 cut(s) 279, 563, 1373, 1392, 1478, 1790
Hpy8I GTNNAC 2 cut(s) 156, 474
HpyAV CCTTC 8 cut(s) 667, 1010, 1065, 1214, 1379, 1776, 1825, 1850
HpyCH4III ACNGT 4 cut(s) 934, 951, 1165, 1504
HpyF10VI GCNNNNNNNGC 5 cut(s) 553, 676, 1181, 1278, 1904
HpyF3I CTNAG 9 cut(s) 141, 371, 386, 401, 527, 645, 729, 1512, 1554
Hsp92I GRCGYC 1 cut(s) 851
HspAI GCGC 2 cut(s) 851, 990
KasI GGCGCC 1 cut(s) 850
KpnI GGTACC 1 cut(s) 2016
Ksp22I TGATCA 1 cut(s) 1063
KspAI GTTAAC 1 cut(s) 156
Kzo9I GATC 6 cut(s) 682, 797, 1063, 1375, 1508, 1578
LguI GCTCTTC 1 cut(s) 442
LmnI GCTCC 7 cut(s) 318, 493, 676, 893, 1239, 1639, 1903
Lsp1109I GCAGC 3 cut(s) 613, 768, 999
MaeI CTAG 4 cut(s) 1392, 1452, 1460, 1545
MaeIII GTNAC 5 cut(s) 134, 617, 998, 1489, 1625
MalI GATC 6 cut(s) 684, 799, 1065, 1377, 1510, 1580
MboI GATC 6 cut(s) 682, 797, 1063, 1375, 1508, 1578
MfeI CAATTG 2 cut(s) 897, 1265
MflI RGATCY 1 cut(s) 1508
MhlI GDGCHC 5 cut(s) 890, 1196, 1244, 1423, 1900
Mly113I GGCGCC 1 cut(s) 851
MlyI GAGTC 1 cut(s) 824
MmeI TCCRAC 3 cut(s) 990, 1122, 1926
Mph1103I ATGCAT 1 cut(s) 1687
MseI TTAA 4 cut(s) 155, 393, 1205, 2023
MslI CAYNNNNRTG 1 cut(s) 496
MspA1I CMGCKG 1 cut(s) 733
MspI CCGG 3 cut(s) 716, 1423, 1637
MspR9I CCNGG 5 cut(s) 837, 891, 1088, 1423, 1728
MunI CAATTG 2 cut(s) 897, 1265
Mva1269I GAATGC 2 cut(s) 1129, 1275
MvaI CCWGG 4 cut(s) 837, 891, 1088, 1728
MwoI GCNNNNNNNGC 5 cut(s) 553, 676, 1181, 1278, 1904
NarI GGCGCC 1 cut(s) 851
NciI CCSGG 1 cut(s) 1423
NcoI CCATGG 2 cut(s) 1848, 1923
NdeII GATC 6 cut(s) 682, 797, 1063, 1375, 1508, 1578
NlaIV GGNNCC 7 cut(s) 409, 708, 852, 1195, 1421, 1635, 2014
NmuCI GTSAC 1 cut(s) 998
NsiI ATGCAT 1 cut(s) 1687
NspI RCATGY 2 cut(s) 247, 1467
PaeI GCATGC 1 cut(s) 1467
PagI TCATGA 1 cut(s) 1372
PceI AGGCCT 1 cut(s) 198
PciI ACATGT 1 cut(s) 243
PciSI GCTCTTC 1 cut(s) 442
PctI GAATGC 2 cut(s) 1129, 1275
PfeI GAWTC 4 cut(s) 514, 1294, 1388, 1843
PflMI CCANNNNNTGG 1 cut(s) 791
PkrI GCNGC 4 cut(s) 603, 783, 989, 1274
PleI GAGTC 1 cut(s) 823
PluTI GGCGCC 1 cut(s) 854
PpsI GAGTC 1 cut(s) 823
PscI ACATGT 1 cut(s) 243
PshAI GACNNNNGTC 1 cut(s) 288
PsiI TTATAA 1 cut(s) 1170
Psp124BI GAGCTC 1 cut(s) 1244
Psp6I CCWGG 4 cut(s) 835, 889, 1086, 1726
PspFI CCCAGC 2 cut(s) 926, 1987
PspGI CCWGG 4 cut(s) 835, 889, 1086, 1726
PspN4I GGNNCC 7 cut(s) 409, 708, 852, 1195, 1421, 1635, 2014
PspOMI GGGCCC 1 cut(s) 1419
PspPI GGNCC 7 cut(s) 261, 408, 554, 707, 1270, 1419, 1420
PstNI CAGNNNCTG 1 cut(s) 607
PsuI RGATCY 1 cut(s) 1508
PvuII CAGCTG 1 cut(s) 733
RsaI GTAC 3 cut(s) 383, 1982, 2014
RsaNI GTAC 3 cut(s) 382, 1981, 2013
RseI CAYNNNNRTG 1 cut(s) 496
SacI GAGCTC 1 cut(s) 1244
SapI GCTCTTC 1 cut(s) 442
SaqAI TTAA 4 cut(s) 155, 393, 1205, 2023
SatI GCNGC 4 cut(s) 602, 782, 988, 1273
Sau3AI GATC 6 cut(s) 682, 797, 1063, 1375, 1508, 1578
Sau96I GGNCC 7 cut(s) 261, 408, 554, 707, 1270, 1419, 1420
SchI GAGTC 1 cut(s) 824
ScrFI CCNGG 5 cut(s) 837, 891, 1088, 1423, 1728
SduI GDGCHC 5 cut(s) 890, 1196, 1244, 1423, 1900
SfcI CTRYAG 2 cut(s) 430, 1587
SfoI GGCGCC 1 cut(s) 852
SinI GGWCC 3 cut(s) 261, 408, 707
SmiMI CAYNNNNRTG 1 cut(s) 496
SmlI CTYRAG 2 cut(s) 419, 856
SmoI CTYRAG 2 cut(s) 419, 856
SphI GCATGC 1 cut(s) 1467
SseBI AGGCCT 1 cut(s) 198
SsiI CCGC 3 cut(s) 1056, 1273, 1737
SspDI GGCGCC 1 cut(s) 850
SspMI CTAG 4 cut(s) 1392, 1452, 1460, 1545
SstI GAGCTC 1 cut(s) 1244
StuI AGGCCT 1 cut(s) 198
StyD4I CCNGG 5 cut(s) 835, 889, 1086, 1421, 1726
StyI CCWWGG 4 cut(s) 1440, 1848, 1923, 1961
TaaI ACNGT 4 cut(s) 934, 951, 1165, 1504
TaqII GACCGA 1 cut(s) 300
TatI WGTACW 1 cut(s) 1980
TauI GCSGC 1 cut(s) 1275
TfiI GAWTC 4 cut(s) 514, 1294, 1388, 1843
Tru1I TTAA 4 cut(s) 155, 393, 1205, 2023
Tru9I TTAA 4 cut(s) 155, 393, 1205, 2023
TscAI CASTG 6 cut(s) 132, 405, 481, 954, 1558, 1776
TseFI GTSAC 1 cut(s) 998
TseI GCWGC 3 cut(s) 601, 781, 987
Tsp45I GTSAC 1 cut(s) 998
TspDTI ATGAA 8 cut(s) 31, 150, 524, 1361, 1401, 1611, 1667, 1944
TspGWI ACGGA 1 cut(s) 1688
TspRI CASTG 6 cut(s) 132, 405, 481, 954, 1558, 1776
Van91I CCANNNNNTGG 1 cut(s) 791
VpaK11BI GGWCC 3 cut(s) 261, 408, 707
XapI RAATTY 4 cut(s) 595, 1288, 1707, 1751
XbaI TCTAGA 1 cut(s) 1391
XceI RCATGY 2 cut(s) 247, 1467
XspI CTAG 4 cut(s) 1392, 1452, 1460, 1545
Zsp2I ATGCAT 1 cut(s) 1687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.