Rh6DG279500

Kinase-like protein TMKL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0015402
Physical Location & Seq
Reverse (-)
47371268 .. 47374481
3214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG279500.1

Sequence Viewer

Length: 2031 bp
ATGGAGATTTTGAAACCCTTGTTTTGCTTTCTGCTCTACTTCATTCTATGCTTCAATGTCTTACCCACTTGGTCTTCTTCTTCTTCTTCTTCTTCAGATGTTGAGCTTCTGTTGGGAAACATCAAGGCTTCACTGCAAGGTAACGCTCAGAACTTGTTGTTAACTTCATGGAACACTTCTCTGCCTGTTTGCCAATGGAGAGGCCTGAAATGGGTTTTCTCCAATGGCTCTCCTTTGCTTTGCAATGACATGTCCTCACCCCATTGGACCAATCTTTCTCTCTCCAGAGACCCAAGTCTTCACCTTTTCTCTCTTCAGCTCCCCTCTGCTAATCTCTCTGGTTCAATACCCAGAGAGATAGGGGAGTTTTCTATGCTCAGAAGCTTGTACCTTAGTGTTAATTCACTGAGTGGGACCATCCCACTTGAGCTTGGCTACAGCTCTTCACTTTCTGAAATTGATTTGGGCAACAATTTGTTCACTGGGGCTTTAGCTCCATCTGTGTGGAACTTGTGTGAGAATCTTGTCTCGCTTAGGCTTCATGGTAATTCGTTATCTGGGCCTGTTCCTGAACCTGCATTGCCCAATTCTACCTGCAAAAATTTGCAGCTACTGGATTTGGGTGACAACAAGTTTTCAGGGAACTTCCCTGAGTTTGTGACCCAGTTTGGTGGGCTGAAGGAGCTTGATCTTGGGAAAAACATGATTTCGGGTCCCGTTCCGGTGAGTTTAGCTCAGCTGGAACTTGACAAGTTGAACCTTTCACACAATAACTTTAGTGGGGTGTTGCCAGCTTTTGGTGGATCAAAGTTTGGTGTGGAGTCTTTTGAGGGGAACAACCCTGGACTTTGTGGGGCGCCCTTGAGAAGTTGTAGTGGAAGCTCTGGGTTGAGCCCTGGAGCAATTGCTGGTATTGTCATTGGTTTGATGGCTGGGACTGTGGTTTTGGCTTCACTGTGTATTGGGTATGTGCAACACAAGAAGAAAAACAGCAGCGCAGAGAGTGACTACGAGTTGGAGGAAGGAGAAGATGAAGAAAATGGCGGTCTTGGTGATGGCGGTGGTGATCAGGGAAGGCTTATATTGTTCCAGGGAGGTGAGCATTTGACATTAGACGATGTGTTGAATGCAACAGGACAAGTGTTGGAGAAGACCAGCTATGGGACTGTTTATAAGGCAAAGCTTGCTGATGGGGGCACCATTACTTTAAGGTTGTTGAGGGAAGGTAGTTGCAAAGATGGGAGCTCTTGTGTGCCTGTAATAAAGCAATTGGGCCACATTCGCTATGAGAATTTGATTCCATTGAGAGCTTTCTATCAGGGGAAGCGAGGGGAGAAGCTTCTGATATATGACCATCTTCCTCTCAAAAGCCTTCATGATCTTTTACATGAATCTAGAGTAGGGAAACCTGTGCTGAACTGGGCTCGGCGACACAAAATTGCCTTGGGTATAGCTAGAGGACTAGCATATCTCCATTCTGGTCTTGAAACACCTGTTACTCATGGGACTGTAAGATCTAAGAATGTGCTTGTGGATGAGTTTTTTGTATCTAGGCTCACTGAGTTCGGGCTCGACAAGGTGATGATCCCCTCTGTAGCAGATGAAATGGTGGCTGTTGCAAAGGCTGATGGTTACAAAGCACCGGAGCTTCAAAAGATGAAGAAGTGCAATTCTCGGACAGATGTTTATGCATTTGGTATTTTGCTATTGGAAATTCTGATAGGCAAGAAGCCTGGTAAAAGCGGGAGGAGTGGCGAATTTGTGGATTTGCCTTCACTGGTCAAAGTGGCAGTTCTGGAGGAGACAACATTGGAGGTTTTCGATGTGGAGGTGTTGAAGGGGATAAGGAATCCCATGGAAGAAGGTTTGGTTCAGGCATTAAAACTTGCAATGGGTTGCTGTGCTCCAGTTGCTTCAGTTAGACCCTCCATGGATGAAGTTGTGAAGCAGTTGGAGGAGAACAGACCAAGGAATAGGTCTGCTTTGTACAGCCCAGCAGAAGAAAGGAGTGAGATTGGTACCCCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

676

Amino Acids

73.06

Weight (kDa)

5.89

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 201 - 259 1.9e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 379 - 651 1e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 386 - 649 2.2e-18 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24660
fragaria_vesca FvH4_2g19550
malus_domestica MD05G1179600.v1.1 MD10G1169200.v1.1
prunus_persica Prupe.8G206900_v2.0.a1
pyrus_communis pycom05g16610 pycom10g14600
rosa_chinensis RchiOBHm_Chr6g0285321
rosa_laevigata RLG00000012654
rosa_multiflora Rmu_sc0011958.1_g000002
rosa_roxburghii Rroxscaffold_7G00183360
rosa_rugosa Rorug06G0173500
rosa_samantha Rh6BG285000 Rh6CG286500 Rh6DG279500
rosa_wichuraiana Rw6G024460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1173
Acc36I ACCTGC 2 cut(s) 583, 602
Acc65I GGTACC 1 cut(s) 2018
AccB1I GGYRCC 3 cut(s) 856, 1196, 2018
AccB7I CCANNNNNTGG 1 cut(s) 797
AciI CCGC 3 cut(s) 1044, 1059, 1743
AclWI GGATC 2 cut(s) 811, 1579
AcsI RAATTY 4 cut(s) 601, 1291, 1713, 1757
AcuI CTGAAG 4 cut(s) 78, 299, 698, 1899
AcyI GRCGYC 1 cut(s) 857
AdeI CACNNNGTG 1 cut(s) 410
AfaI GTAC 3 cut(s) 389, 1988, 2020
AfiI CCNNNNNNNGG 3 cut(s) 211, 797, 1161
AflIII ACRYGT 1 cut(s) 249
AgsI TTSAA 8 cut(s) 13, 55, 345, 757, 1126, 1487, 1652, 1837
AjnI CCWGG 4 cut(s) 841, 895, 1089, 1732
Alw21I GWGCWC 2 cut(s) 1247, 1906
Alw26I GTCTC 3 cut(s) 282, 532, 1796
AlwI GGATC 2 cut(s) 811, 1579
AlwNI CAGNNNCTG 1 cut(s) 613
AoxI GGCC 3 cut(s) 202, 560, 1273
ApeKI GCWGC 2 cut(s) 607, 993
ApoI RAATTY 4 cut(s) 601, 1291, 1713, 1757
ArsI GACNNNNNNTTYG 2 cut(s) 928, 960
Asp718I GGTACC 1 cut(s) 2018
AspLEI GCGC 2 cut(s) 859, 998
AspS9I GGNCC 5 cut(s) 267, 414, 560, 713, 1273
AsuHPI GGTGA 8 cut(s) 249, 293, 635, 736, 1064, 1076, 1109, 1591
AvaII GGWCC 3 cut(s) 267, 414, 713
BaeGI GKGCMC 1 cut(s) 1199
BanI GGYRCC 3 cut(s) 856, 1196, 2018
BanII GRGCYC 4 cut(s) 896, 1247, 1426, 1572
BbsI GAAGAC 3 cut(s) 66, 290, 1157
Bbv12I GWGCWC 2 cut(s) 1247, 1906
BbvI GCAGC 2 cut(s) 619, 1005
BccI CCATC 8 cut(s) 425, 505, 922, 1049, 1184, 1232, 1362, 1622
BciT130I CCWGG 4 cut(s) 843, 897, 1091, 1734
BclI TGATCA 1 cut(s) 1066
BcoDI GTCTC 3 cut(s) 282, 532, 1796
BfaI CTAG 4 cut(s) 1395, 1455, 1463, 1551
BfmI CTRYAG 2 cut(s) 436, 1593
BfoI RGCGCY 1 cut(s) 860
BfuAI ACCTGC 2 cut(s) 583, 602
BglII AGATCT 1 cut(s) 1514
BisI GCNGC 2 cut(s) 608, 994
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 2 cut(s) 609, 995
Bme1390I CCNGG 4 cut(s) 843, 897, 1091, 1734
Bme18I GGWCC 3 cut(s) 267, 414, 713
BmgT120I GGNCC 5 cut(s) 267, 414, 560, 713, 1273
BmiI GGNNCC 6 cut(s) 415, 714, 715, 858, 1198, 2020
BmrFI CCNGG 4 cut(s) 843, 897, 1091, 1734
BmrI ACTGGG 3 cut(s) 492, 658, 1429
BmuI ACTGGG 3 cut(s) 492, 658, 1429
BoxI GACNNNNGTC 1 cut(s) 294
BpiI GAAGAC 3 cut(s) 66, 290, 1157
BplI GAGNNNNNCTC 2 cut(s) 718, 750
BpmI CTGGAG 4 cut(s) 268, 918, 1817, 1890
Bpu10I CCTNAGC 1 cut(s) 533
Bpu1102I GCTNAGC 1 cut(s) 735
BpuEI CTTGAG 2 cut(s) 446, 883
BsaHI GRCGYC 1 cut(s) 857
BsaI GGTCTC 1 cut(s) 282
BsaJI CCNNGG 7 cut(s) 841, 895, 1090, 1443, 1854, 1929, 1967
BsaWI WCCGGW 2 cut(s) 721, 1642
BsaXI ACNNNNNCTCC 2 cut(s) 1234, 1264
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 797, 1161
Bse1I ACTGG 6 cut(s) 487, 618, 664, 1424, 1782, 1907
Bse3DI GCAATG 3 cut(s) 250, 578, 1896
BseBI CCWGG 4 cut(s) 843, 897, 1091, 1734
BseDI CCNNGG 7 cut(s) 841, 895, 1090, 1443, 1854, 1929, 1967
BseGI GGATG 3 cut(s) 417, 1540, 1939
BseLI CCNNNNNNNGG 3 cut(s) 211, 797, 1161
BseMI GCAATG 3 cut(s) 250, 578, 1896
BseMII CTCAG 6 cut(s) 161, 391, 398, 642, 749, 1551
BseNI ACTGG 6 cut(s) 487, 618, 664, 1424, 1782, 1907
BseRI GAGGAG 3 cut(s) 1762, 1814, 1970
BseSI GKGCMC 1 cut(s) 1199
BseXI GCAGC 2 cut(s) 619, 1005
BseYI CCCAGC 2 cut(s) 932, 1993
BshFI GGCC 3 cut(s) 204, 562, 1275
BshNI GGYRCC 3 cut(s) 856, 1196, 2018
BsiHKAI GWGCWC 2 cut(s) 1247, 1906
BsiSI CCGG 2 cut(s) 722, 1643
BslFI GGGAC 5 cut(s) 427, 699, 949, 1177, 1519
BslI CCNNNNNNNGG 3 cut(s) 211, 797, 1161
BsmAI GTCTC 3 cut(s) 282, 532, 1796
BsmFI GGGAC 5 cut(s) 427, 699, 949, 1177, 1519
BsmI GAATGC 1 cut(s) 1132
BsnI GGCC 3 cut(s) 204, 562, 1275
Bso31I GGTCTC 1 cut(s) 282
Bsp1286I GDGCHC 6 cut(s) 896, 1199, 1247, 1426, 1572, 1906
Bsp1407I TGTACA 1 cut(s) 1986
Bsp143I GATC 6 cut(s) 688, 803, 1066, 1378, 1514, 1584
Bsp1720I GCTNAGC 1 cut(s) 735
Bsp19I CCATGG 2 cut(s) 1854, 1929
BspACI CCGC 3 cut(s) 1044, 1059, 1743
BspANI GGCC 3 cut(s) 204, 562, 1275
BspCNI CTCAG 6 cut(s) 160, 390, 399, 643, 748, 1552
BspHI TCATGA 1 cut(s) 1375
BspLI GGNNCC 6 cut(s) 415, 714, 715, 858, 1198, 2020
BspMI ACCTGC 2 cut(s) 583, 602
BspPI GGATC 2 cut(s) 811, 1579
BspQI GCTCTTC 1 cut(s) 448
BspT107I GGYRCC 3 cut(s) 856, 1196, 2018
BspTNI GGTCTC 1 cut(s) 282
BsrDI GCAATG 3 cut(s) 250, 578, 1896
BsrGI TGTACA 1 cut(s) 1986
BsrI ACTGG 6 cut(s) 487, 618, 664, 1424, 1782, 1907
BssECI CCNNGG 7 cut(s) 841, 895, 1090, 1443, 1854, 1929, 1967
BssMI GATC 6 cut(s) 688, 803, 1066, 1378, 1514, 1584
BssNI GRCGYC 1 cut(s) 857
BssT1I CCWWGG 4 cut(s) 1443, 1854, 1929, 1967
Bst2UI CCWGG 4 cut(s) 843, 897, 1091, 1734
Bst4CI ACNGT 4 cut(s) 940, 957, 1168, 1510
Bst6I CTCTTC 2 cut(s) 318, 448
BstACI GRCGYC 1 cut(s) 857
BstAPI GCANNNNNTGC 1 cut(s) 1184
BstAUI TGTACA 1 cut(s) 1986
BstC8I GCNNGC 2 cut(s) 792, 1185
BstDEI CTNAG 9 cut(s) 147, 377, 392, 407, 533, 651, 735, 1518, 1560
BstDSI CCRYGG 2 cut(s) 1854, 1929
BstF5I GGATG 3 cut(s) 417, 1540, 1939
BstH2I RGCGCY 1 cut(s) 860
BstHHI GCGC 2 cut(s) 859, 998
BstKTI GATC 6 cut(s) 691, 806, 1069, 1381, 1517, 1587
BstMAI GTCTC 3 cut(s) 282, 532, 1796
BstMBI GATC 6 cut(s) 688, 803, 1066, 1378, 1514, 1584
BstMWI GCNNNNNNNGC 4 cut(s) 682, 1184, 1281, 1910
BstNI CCWGG 4 cut(s) 843, 897, 1091, 1734
BstNSI RCATGY 1 cut(s) 253
BstPAI GACNNNNGTC 1 cut(s) 294
BstSCI CCNGG 4 cut(s) 841, 895, 1089, 1732
BstSFI CTRYAG 2 cut(s) 436, 1593
BstSLI GKGCMC 1 cut(s) 1199
BstV1I GCAGC 2 cut(s) 619, 1005
BstV2I GAAGAC 3 cut(s) 66, 290, 1157
BstX2I RGATCY 1 cut(s) 1514
BstXI CCANNNNNNTGG 2 cut(s) 504, 671
BstYI RGATCY 1 cut(s) 1514
BsuRI GGCC 3 cut(s) 204, 562, 1275
BtgI CCRYGG 2 cut(s) 1854, 1929
BtsCI GGATG 3 cut(s) 417, 1540, 1939
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 6 cut(s) 131, 404, 480, 953, 1557, 1775
BveI ACCTGC 2 cut(s) 583, 602
Cac8I GCNNGC 2 cut(s) 792, 1185
CaiI CAGNNNCTG 1 cut(s) 613
CciI TCATGA 1 cut(s) 1375
CfoI GCGC 2 cut(s) 859, 998
Cfr13I GGNCC 5 cut(s) 267, 414, 560, 713, 1273
Csp6I GTAC 3 cut(s) 388, 1987, 2019
CviAII CATG 9 cut(s) 168, 250, 542, 703, 1376, 1388, 1502, 1855, 1930
CviQI GTAC 3 cut(s) 388, 1987, 2019
DdeI CTNAG 9 cut(s) 147, 377, 392, 407, 533, 651, 735, 1518, 1560
DinI GGCGCC 1 cut(s) 858
DpnI GATC 6 cut(s) 690, 805, 1068, 1380, 1516, 1586
DpnII GATC 6 cut(s) 688, 803, 1066, 1378, 1514, 1584
DraIII CACNNNGTG 1 cut(s) 410
Eam1104I CTCTTC 2 cut(s) 318, 448
EarI CTCTTC 2 cut(s) 318, 448
Ecl136II GAGCTC 1 cut(s) 1245
Eco130I CCWWGG 4 cut(s) 1443, 1854, 1929, 1967
Eco147I AGGCCT 1 cut(s) 204
Eco24I GRGCYC 4 cut(s) 896, 1247, 1426, 1572
Eco31I GGTCTC 1 cut(s) 282
Eco47I GGWCC 3 cut(s) 267, 414, 713
Eco53kI GAGCTC 1 cut(s) 1245
Eco57I CTGAAG 4 cut(s) 78, 299, 698, 1899
EcoICRI GAGCTC 1 cut(s) 1245
EcoO109I RGGNCCY 1 cut(s) 713
EcoRII CCWGG 4 cut(s) 841, 895, 1089, 1732
EcoT14I CCWWGG 4 cut(s) 1443, 1854, 1929, 1967
EcoT22I ATGCAT 1 cut(s) 1693
EcoT38I GRGCYC 4 cut(s) 896, 1247, 1426, 1572
EgeI GGCGCC 1 cut(s) 858
EheI GGCGCC 1 cut(s) 858
ErhI CCWWGG 4 cut(s) 1443, 1854, 1929, 1967
FaeI CATG 9 cut(s) 171, 253, 545, 706, 1379, 1391, 1505, 1858, 1933
FaqI GGGAC 5 cut(s) 427, 699, 949, 1177, 1519
FatI CATG 9 cut(s) 167, 249, 541, 702, 1375, 1387, 1501, 1854, 1929
FauI CCCGC 1 cut(s) 1736
FbaI TGATCA 1 cut(s) 1066
Fnu4HI GCNGC 2 cut(s) 608, 994
FokI GGATG 3 cut(s) 404, 1547, 1946
FriOI GRGCYC 4 cut(s) 896, 1247, 1426, 1572
Fsp4HI GCNGC 2 cut(s) 608, 994
FspBI CTAG 4 cut(s) 1395, 1455, 1463, 1551
GlaI GCGC 2 cut(s) 858, 997
GluI GCNGC 2 cut(s) 608, 994
GsaI CCCAGC 2 cut(s) 936, 1997
GsuI CTGGAG 4 cut(s) 268, 918, 1817, 1890
HaeII RGCGCY 1 cut(s) 860
HaeIII GGCC 3 cut(s) 204, 562, 1275
HapII CCGG 2 cut(s) 722, 1643
HhaI GCGC 2 cut(s) 859, 998
Hin1I GRCGYC 1 cut(s) 857
Hin1II CATG 9 cut(s) 171, 253, 545, 706, 1379, 1391, 1505, 1858, 1933
Hin6I GCGC 2 cut(s) 857, 996
HinP1I GCGC 2 cut(s) 857, 996
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HindIII AAGCTT 3 cut(s) 382, 1181, 1337
HinfI GANTC 5 cut(s) 520, 821, 1297, 1391, 1849
HpaI GTTAAC 1 cut(s) 162
HpaII CCGG 2 cut(s) 722, 1643
HphI GGTGA 8 cut(s) 249, 293, 635, 736, 1064, 1076, 1109, 1591
Hpy166II GTNNAC 2 cut(s) 162, 480
Hpy188I TCNGA 7 cut(s) 97, 150, 380, 454, 1344, 1677, 1719
Hpy188III TCNNGA 6 cut(s) 285, 569, 1376, 1395, 1484, 1796
Hpy8I GTNNAC 2 cut(s) 162, 480
HpyAV CCTTC 8 cut(s) 673, 1016, 1068, 1217, 1382, 1782, 1831, 1856
HpyCH4III ACNGT 4 cut(s) 940, 957, 1168, 1510
HpyF10VI GCNNNNNNNGC 4 cut(s) 682, 1184, 1281, 1910
HpyF3I CTNAG 9 cut(s) 147, 377, 392, 407, 533, 651, 735, 1518, 1560
Hsp92I GRCGYC 1 cut(s) 857
Hsp92II CATG 9 cut(s) 171, 253, 545, 706, 1379, 1391, 1505, 1858, 1933
HspAI GCGC 2 cut(s) 857, 996
KasI GGCGCC 1 cut(s) 856
KflI GGGWCCC 1 cut(s) 713
KpnI GGTACC 1 cut(s) 2022
Ksp22I TGATCA 1 cut(s) 1066
KspAI GTTAAC 1 cut(s) 162
Kzo9I GATC 6 cut(s) 688, 803, 1066, 1378, 1514, 1584
LguI GCTCTTC 1 cut(s) 448
LmnI GCTCC 7 cut(s) 324, 499, 682, 899, 1242, 1645, 1909
Lsp1109I GCAGC 2 cut(s) 619, 1005
MaeI CTAG 4 cut(s) 1395, 1455, 1463, 1551
MaeIII GTNAC 6 cut(s) 140, 623, 658, 1004, 1495, 1631
MalI GATC 6 cut(s) 690, 805, 1068, 1380, 1516, 1586
MboI GATC 6 cut(s) 688, 803, 1066, 1378, 1514, 1584
MfeI CAATTG 2 cut(s) 903, 1268
MflI RGATCY 1 cut(s) 1514
MhlI GDGCHC 6 cut(s) 896, 1199, 1247, 1426, 1572, 1906
Mly113I GGCGCC 1 cut(s) 857
MlyI GAGTC 1 cut(s) 830
MmeI TCCRAC 3 cut(s) 996, 1125, 1932
Mph1103I ATGCAT 1 cut(s) 1693
MseI TTAA 5 cut(s) 161, 399, 1208, 1880, 2029
MslI CAYNNNNRTG 1 cut(s) 502
MspA1I CMGCKG 1 cut(s) 739
MspI CCGG 2 cut(s) 722, 1643
MspR9I CCNGG 4 cut(s) 843, 897, 1091, 1734
MunI CAATTG 2 cut(s) 903, 1268
Mva1269I GAATGC 1 cut(s) 1132
MvaI CCWGG 4 cut(s) 843, 897, 1091, 1734
MwoI GCNNNNNNNGC 4 cut(s) 682, 1184, 1281, 1910
NarI GGCGCC 1 cut(s) 857
NcoI CCATGG 2 cut(s) 1854, 1929
NdeII GATC 6 cut(s) 688, 803, 1066, 1378, 1514, 1584
NlaIII CATG 9 cut(s) 171, 253, 545, 706, 1379, 1391, 1505, 1858, 1933
NlaIV GGNNCC 6 cut(s) 415, 714, 715, 858, 1198, 2020
NmeAIII GCCGAG 1 cut(s) 1405
NmuCI GTSAC 3 cut(s) 623, 658, 1004
NsiI ATGCAT 1 cut(s) 1693
NspI RCATGY 1 cut(s) 253
PagI TCATGA 1 cut(s) 1375
PceI AGGCCT 1 cut(s) 204
PciI ACATGT 1 cut(s) 249
PciSI GCTCTTC 1 cut(s) 448
PctI GAATGC 1 cut(s) 1132
PfeI GAWTC 4 cut(s) 520, 1297, 1391, 1849
PflMI CCANNNNNTGG 1 cut(s) 797
PkrI GCNGC 2 cut(s) 609, 995
PleI GAGTC 1 cut(s) 829
PluTI GGCGCC 1 cut(s) 860
PpsI GAGTC 1 cut(s) 829
PpuMI RGGWCCY 1 cut(s) 713
PscI ACATGT 1 cut(s) 249
PshAI GACNNNNGTC 1 cut(s) 294
PsiI TTATAA 1 cut(s) 1173
Psp124BI GAGCTC 1 cut(s) 1247
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 4 cut(s) 841, 895, 1089, 1732
PspFI CCCAGC 2 cut(s) 932, 1993
PspGI CCWGG 4 cut(s) 841, 895, 1089, 1732
PspN4I GGNNCC 6 cut(s) 415, 714, 715, 858, 1198, 2020
PspPI GGNCC 5 cut(s) 267, 414, 560, 713, 1273
PspPPI RGGWCCY 1 cut(s) 713
PstNI CAGNNNCTG 1 cut(s) 613
PsuI RGATCY 1 cut(s) 1514
PvuII CAGCTG 1 cut(s) 739
RsaI GTAC 3 cut(s) 389, 1988, 2020
RsaNI GTAC 3 cut(s) 388, 1987, 2019
RseI CAYNNNNRTG 1 cut(s) 502
SacI GAGCTC 1 cut(s) 1247
SapI GCTCTTC 1 cut(s) 448
SaqAI TTAA 5 cut(s) 161, 399, 1208, 1880, 2029
SatI GCNGC 2 cut(s) 608, 994
Sau3AI GATC 6 cut(s) 688, 803, 1066, 1378, 1514, 1584
Sau96I GGNCC 5 cut(s) 267, 414, 560, 713, 1273
SchI GAGTC 1 cut(s) 830
ScrFI CCNGG 4 cut(s) 843, 897, 1091, 1734
SduI GDGCHC 6 cut(s) 896, 1199, 1247, 1426, 1572, 1906
SfcI CTRYAG 2 cut(s) 436, 1593
SfoI GGCGCC 1 cut(s) 858
SinI GGWCC 3 cut(s) 267, 414, 713
SmiMI CAYNNNNRTG 1 cut(s) 502
SmlI CTYRAG 2 cut(s) 425, 862
SmoI CTYRAG 2 cut(s) 425, 862
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 3 cut(s) 1044, 1059, 1743
SspDI GGCGCC 1 cut(s) 856
SspMI CTAG 4 cut(s) 1395, 1455, 1463, 1551
SstI GAGCTC 1 cut(s) 1247
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 4 cut(s) 841, 895, 1089, 1732
StyI CCWWGG 4 cut(s) 1443, 1854, 1929, 1967
TaaI ACNGT 4 cut(s) 940, 957, 1168, 1510
TaqI TCGA 2 cut(s) 1572, 1821
TatI WGTACW 1 cut(s) 1986
TfiI GAWTC 4 cut(s) 520, 1297, 1391, 1849
Tru1I TTAA 5 cut(s) 161, 399, 1208, 1880, 2029
Tru9I TTAA 5 cut(s) 161, 399, 1208, 1880, 2029
TscAI CASTG 6 cut(s) 138, 411, 487, 960, 1564, 1782
TseFI GTSAC 3 cut(s) 623, 658, 1004
TseI GCWGC 2 cut(s) 607, 993
Tsp45I GTSAC 3 cut(s) 623, 658, 1004
TspDTI ATGAA 9 cut(s) 31, 156, 530, 1047, 1364, 1404, 1617, 1673, 1950
TspRI CASTG 6 cut(s) 138, 411, 487, 960, 1564, 1782
Van91I CCANNNNNTGG 1 cut(s) 797
VpaK11BI GGWCC 3 cut(s) 267, 414, 713
XapI RAATTY 4 cut(s) 601, 1291, 1713, 1757
XbaI TCTAGA 1 cut(s) 1394
XceI RCATGY 1 cut(s) 253
XspI CTAG 4 cut(s) 1395, 1455, 1463, 1551
Zsp2I ATGCAT 1 cut(s) 1693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.