Rorug06G0173500

Kinase-like protein TMKL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008200
Physical Location & Seq
Reverse (-)
25577882 .. 25582371
4490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0173500.1

Sequence Viewer

Length: 1338 bp
ATGGATGAAGAGTACGACGTGATTGTTTTAGGGACGGGTCTCAAGGAATGCATTCTCAGTGGCCTTCTCTCTGTTGATGGCCTCAAAGTTTTGCATATGGATAGAAATGACTACTATGGAGGAGCATCGACCTCTCTTAATCTTACACAACTTTGGAAGCGTTTTAGAGGAGACGACAAACCTCCAGAGCAATTGGGGTCTAGTAAGGAGTACAACGTTGACATGATACCAAAGTTCATGATGGCCAATGGCGGTTTGGTCCGTGTCCTAATCCACACAAATGTCACAAAGTATCTTAATTTTAAGGCTGTTGATGGTAGTTTTGTGTATAACAAGAAAAAGATCTATAAAGTTCCAGCAACTGATGTTGAAGCACTAAAATCACCATTGATGGGGCTCTTTGAAAAGCGTCGTGCACGAAAGTTCTTCATTTATGTCCAAGACTTTGAAGAGAGTGATCCCAAATCTCATGAAGGACTAGATTTGCATAAAGTTACAGCAAGAGAGCTTATCTCGAAATATGAGCTAGAGGATGATACTATTGATTTTATTGGTCATGCTTTGGCACTTCATATTGATGATTCTTACCTAGATGAGCCAGCCATGGAGTTTGTGAAGAAAATGAAGCTCTATGCAGAGTCTCTGGCACGTTTTCAAGGAGGATCGCCTTATATCTATCCATTGTATGGATTGGGGGAGTTGCCTCAGGCTTTTGCACGTTTAAGTGCAGTTTATGGTGGAACTTACATGCTCAACAAACCGGATTGTAAGGTGAAATTTGATGATAATGGAAAGGCTGTTGGTGTGACCTCAGAAGGAGAAACCGCTAAATGCAAGAAAGTGGTTTGTGATCCCTCGTATTTGCCTGATAAGGTACAGAAAATTGGAAAAGTTGCTCGTGCTATATGTATAATGAGTCATCCTATTCCGGAGACAGATGATTCTCACTCGGTCCAGGTTATTTTGCCACAGAAGCAACTTGGTCGTAAATCAGATATGTACCTGTTTTGTTGCTCTTATGCTCATAATGTAGCTCCAAAAGGAAAATTCATCGCCTTTGTTTCAACGATAGCAGAGACCGATAATCCTGAAGTTGAATTAAAGCCTGGCATTGCTCTACTTGGTCCTATAGATGAAATCTTCTATGACTCTTATGACAGATACATACCCAATAATGACCATGAAGGCGACAGTTGCTACATATCTACCAGTTATGATCCAACTACGCACTTTGAGTCCACAGTAAATGATGTTCTAGCCATGTATAGTAAGATTACTGGAAAGGCTCTTGATCTTTCTGTGGATCTAAGTGCTGCAAGTGCTGCTACTAAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

445

Amino Acids

49.7

Weight (kDa)

5.49

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDI PF00996 1 - 433 5.9e-226 GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 686
AccIII TCCGGA 1 cut(s) 928
AciI CCGC 2 cut(s) 252, 825
AclI AACGTT 1 cut(s) 216
AclWI GGATC 5 cut(s) 452, 670, 845, 1211, 1311
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 2 cut(s) 776, 1046
AcuI CTGAAG 1 cut(s) 1110
AfaI GTAC 4 cut(s) 14, 212, 876, 1001
AfiI CCNNNNNNNGG 2 cut(s) 392, 686
AgsI TTSAA 6 cut(s) 371, 404, 449, 656, 1065, 1097
AjiI CACGTC 1 cut(s) 19
AjnI CCWGG 2 cut(s) 954, 1105
AluBI AGCT 4 cut(s) 508, 526, 628, 1034
AluI AGCT 4 cut(s) 508, 526, 628, 1034
Alw21I GWGCWC 1 cut(s) 418
Alw26I GTCTC 5 cut(s) 44, 165, 645, 926, 1070
Alw44I GTGCAC 1 cut(s) 414
AlwI GGATC 5 cut(s) 452, 670, 845, 1211, 1311
AlwNI CAGNNNCTG 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 928
AoxI GGCC 3 cut(s) 61, 79, 243
ApaLI GTGCAC 1 cut(s) 414
ApeKI GCWGC 2 cut(s) 1313, 1322
ApoI RAATTY 2 cut(s) 776, 1046
Asp700I GAANNNNTTC 1 cut(s) 51
AspS9I GGNCC 3 cut(s) 259, 952, 1124
AsuHPI GGTGA 2 cut(s) 375, 784
AvaII GGWCC 3 cut(s) 259, 952, 1124
AxyI CCTNAGG 1 cut(s) 705
BaeGI GKGCMC 1 cut(s) 418
BaeI ACNNNNGTAYC 2 cut(s) 218, 251
BalI TGGCCA 1 cut(s) 245
BanII GRGCYC 1 cut(s) 399
BauI CACGAG 1 cut(s) 897
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 1300, 1309
BccI CCATC 4 cut(s) 71, 235, 308, 385
BcgI CGANNNNNNTGC 2 cut(s) 965, 999
BciT130I CCWGG 2 cut(s) 956, 1107
BcoDI GTCTC 5 cut(s) 44, 165, 645, 926, 1070
BfaI CTAG 5 cut(s) 201, 479, 527, 590, 1256
BfmI CTRYAG 1 cut(s) 1128
BglII AGATCT 1 cut(s) 342
BisI GCNGC 2 cut(s) 1314, 1323
BlsI GCNGC 2 cut(s) 1315, 1324
Bme1390I CCNGG 2 cut(s) 956, 1107
Bme18I GGWCC 3 cut(s) 259, 952, 1124
BmgBI CACGTC 1 cut(s) 19
BmgT120I GGNCC 3 cut(s) 259, 952, 1124
BmrFI CCNGG 2 cut(s) 956, 1107
BmsI GCATC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 497, 529
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 26
BsaI GGTCTC 2 cut(s) 44, 1070
BsaJI CCNNGG 1 cut(s) 603
BsaWI WCCGGW 2 cut(s) 760, 928
Bsc4I CCNNNNNNNGG 2 cut(s) 392, 686
Bse1I ACTGG 2 cut(s) 1209, 1282
Bse21I CCTNAGG 1 cut(s) 705
Bse3DI GCAATG 1 cut(s) 1110
BseAI TCCGGA 1 cut(s) 928
BseBI CCWGG 2 cut(s) 956, 1107
BseDI CCNNGG 1 cut(s) 603
BseGI GGATG 3 cut(s) 10, 538, 919
BseLI CCNNNNNNNGG 2 cut(s) 392, 686
BseMI GCAATG 1 cut(s) 1110
BseMII CTCAG 3 cut(s) 70, 719, 825
BseNI ACTGG 2 cut(s) 1209, 1282
BseRI GAGGAG 2 cut(s) 135, 183
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 2 cut(s) 1300, 1309
BsgI GTGCAG 1 cut(s) 747
BshFI GGCC 3 cut(s) 63, 81, 245
BsiHKAI GWGCWC 1 cut(s) 418
BsiSI CCGG 2 cut(s) 761, 929
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 2 cut(s) 392, 686
BsmAI GTCTC 5 cut(s) 44, 165, 645, 926, 1070
BsmBI CGTCTC 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 46
BsmI GAATGC 2 cut(s) 51, 53
BsnI GGCC 3 cut(s) 63, 81, 245
Bso31I GGTCTC 2 cut(s) 44, 1070
Bsp1286I GDGCHC 2 cut(s) 399, 418
Bsp13I TCCGGA 1 cut(s) 928
Bsp143I GATC 7 cut(s) 342, 457, 662, 850, 1216, 1291, 1303
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 2 cut(s) 252, 825
BspANI GGCC 3 cut(s) 63, 81, 245
BspCNI CTCAG 3 cut(s) 69, 718, 824
BspEI TCCGGA 1 cut(s) 928
BspHI TCATGA 2 cut(s) 237, 469
BspPI GGATC 5 cut(s) 452, 670, 845, 1211, 1311
BspTNI GGTCTC 2 cut(s) 44, 1070
BsrDI GCAATG 1 cut(s) 1110
BsrI ACTGG 2 cut(s) 1209, 1282
BssECI CCNNGG 1 cut(s) 603
BssMI GATC 7 cut(s) 342, 457, 662, 850, 1216, 1291, 1303
BssSI CACGAG 1 cut(s) 897
BssT1I CCWWGG 1 cut(s) 603
Bst2BI CACGAG 1 cut(s) 897
Bst2UI CCWGG 2 cut(s) 956, 1107
Bst4CI ACNGT 2 cut(s) 1193, 1243
Bst6I CTCTTC 2 cut(s) 3, 444
BstAPI GCANNNNNTGC 1 cut(s) 1322
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 4 cut(s) 56, 705, 811, 1307
BstDSI CCRYGG 1 cut(s) 603
BstF5I GGATG 3 cut(s) 10, 538, 919
BstKTI GATC 7 cut(s) 345, 460, 665, 853, 1219, 1294, 1306
BstMAI GTCTC 5 cut(s) 44, 165, 645, 926, 1070
BstMBI GATC 7 cut(s) 342, 457, 662, 850, 1216, 1291, 1303
BstMWI GCNNNNNNNGC 4 cut(s) 973, 1194, 1319, 1322
BstNI CCWGG 2 cut(s) 956, 1107
BstNSI RCATGY 1 cut(s) 751
BstSCI CCNGG 2 cut(s) 954, 1105
BstSFI CTRYAG 1 cut(s) 1128
BstSLI GKGCMC 1 cut(s) 418
BstV1I GCAGC 2 cut(s) 1300, 1309
BstX2I RGATCY 2 cut(s) 342, 1303
BstYI RGATCY 2 cut(s) 342, 1303
Bsu36I CCTNAGG 1 cut(s) 705
BsuRI GGCC 3 cut(s) 63, 81, 245
BtgI CCRYGG 1 cut(s) 603
BtgZI GCGATG 1 cut(s) 1036
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 3 cut(s) 10, 538, 919
BtsIMutI CAGTG 1 cut(s) 64
Cac8I GCNNGC 1 cut(s) 600
CaiI CAGNNNCTG 1 cut(s) 362
CciI TCATGA 2 cut(s) 237, 469
Cfr13I GGNCC 3 cut(s) 259, 952, 1124
CseI GACGC 1 cut(s) 398
Csp6I GTAC 4 cut(s) 13, 211, 875, 1000
CviAII CATG 8 cut(s) 223, 238, 470, 557, 604, 748, 1181, 1261
CviQI GTAC 4 cut(s) 13, 211, 875, 1000
DdeI CTNAG 4 cut(s) 56, 705, 811, 1307
DpnI GATC 7 cut(s) 344, 459, 664, 852, 1218, 1293, 1305
DpnII GATC 7 cut(s) 342, 457, 662, 850, 1216, 1291, 1303
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 2 cut(s) 3, 444
EarI CTCTTC 2 cut(s) 3, 444
Eco130I CCWWGG 1 cut(s) 603
Eco24I GRGCYC 1 cut(s) 399
Eco31I GGTCTC 2 cut(s) 44, 1070
Eco47I GGWCC 3 cut(s) 259, 952, 1124
Eco57I CTGAAG 1 cut(s) 1110
Eco81I CCTNAGG 1 cut(s) 705
EcoRII CCWGG 2 cut(s) 954, 1105
EcoT14I CCWWGG 1 cut(s) 603
EcoT22I ATGCAT 1 cut(s) 53
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 603
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 8 cut(s) 226, 241, 473, 560, 607, 751, 1184, 1264
FaqI GGGAC 1 cut(s) 46
FatI CATG 8 cut(s) 222, 237, 469, 556, 603, 747, 1180, 1260
FauNDI CATATG 1 cut(s) 96
Fnu4HI GCNGC 2 cut(s) 1314, 1323
FokI GGATG 3 cut(s) 17, 545, 906
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 2 cut(s) 1314, 1323
FspBI CTAG 5 cut(s) 201, 479, 527, 590, 1256
GluI GCNGC 2 cut(s) 1314, 1323
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 3 cut(s) 63, 81, 245
HapII CCGG 2 cut(s) 761, 929
HgaI GACGC 1 cut(s) 398
Hin1II CATG 8 cut(s) 226, 241, 473, 560, 607, 751, 1184, 1264
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 6 cut(s) 581, 638, 916, 941, 1148, 1235
HpaII CCGG 2 cut(s) 761, 929
HphI GGTGA 2 cut(s) 375, 784
Hpy166II GTNNAC 3 cut(s) 220, 416, 1239
Hpy188I TCNGA 2 cut(s) 814, 994
Hpy188III TCNNGA 7 cut(s) 185, 238, 470, 514, 929, 1088, 1289
Hpy8I GTNNAC 3 cut(s) 220, 416, 1239
Hpy99I CGWCG 2 cut(s) 20, 414
HpyAV CCTTC 4 cut(s) 74, 467, 809, 1178
HpyCH4III ACNGT 2 cut(s) 1193, 1243
HpyCH4IV ACGT 4 cut(s) 18, 216, 649, 718
HpyCH4V TGCA 9 cut(s) 51, 94, 416, 487, 635, 716, 728, 834, 1316
HpyF10VI GCNNNNNNNGC 4 cut(s) 973, 1194, 1319, 1322
HpyF3I CTNAG 4 cut(s) 56, 705, 811, 1307
HpySE526I ACGT 4 cut(s) 18, 216, 649, 718
Hsp92II CATG 8 cut(s) 226, 241, 473, 560, 607, 751, 1184, 1264
Kpn2I TCCGGA 1 cut(s) 928
Kzo9I GATC 7 cut(s) 342, 457, 662, 850, 1216, 1291, 1303
LmnI GCTCC 2 cut(s) 122, 1039
Lsp1109I GCAGC 2 cut(s) 1300, 1309
LweI GCATC 1 cut(s) 134
MaeI CTAG 5 cut(s) 201, 479, 527, 590, 1256
MaeII ACGT 4 cut(s) 18, 216, 649, 718
MaeIII GTNAC 3 cut(s) 283, 493, 805
MalI GATC 7 cut(s) 344, 459, 664, 852, 1218, 1293, 1305
MboI GATC 7 cut(s) 342, 457, 662, 850, 1216, 1291, 1303
MboII GAAGA 5 cut(s) 20, 418, 461, 628, 1132
MfeI CAATTG 1 cut(s) 191
MflI RGATCY 2 cut(s) 342, 1303
MhlI GDGCHC 2 cut(s) 399, 418
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 6 cut(s) 191, 298, 776, 882, 1046, 1097
MluNI TGGCCA 1 cut(s) 245
MlyI GAGTC 4 cut(s) 647, 925, 1142, 1244
MmeI TCCRAC 1 cut(s) 1244
Mox20I TGGCCA 1 cut(s) 245
Mph1103I ATGCAT 1 cut(s) 53
MroI TCCGGA 1 cut(s) 928
MroXI GAANNNNTTC 1 cut(s) 51
MscI TGGCCA 1 cut(s) 245
MseI TTAA 5 cut(s) 138, 297, 303, 722, 1100
MslI CAYNNNNRTG 2 cut(s) 279, 576
Msp20I TGGCCA 1 cut(s) 245
MspI CCGG 2 cut(s) 761, 929
MspR9I CCNGG 2 cut(s) 956, 1107
MunI CAATTG 1 cut(s) 191
Mva1269I GAATGC 2 cut(s) 51, 53
MvaI CCWGG 2 cut(s) 956, 1107
MwoI GCNNNNNNNGC 4 cut(s) 973, 1194, 1319, 1322
NcoI CCATGG 1 cut(s) 603
NdeI CATATG 1 cut(s) 96
NdeII GATC 7 cut(s) 342, 457, 662, 850, 1216, 1291, 1303
NlaIII CATG 8 cut(s) 226, 241, 473, 560, 607, 751, 1184, 1264
NmuCI GTSAC 2 cut(s) 283, 805
NsiI ATGCAT 1 cut(s) 53
NspI RCATGY 1 cut(s) 751
PagI TCATGA 2 cut(s) 237, 469
PctI GAATGC 2 cut(s) 51, 53
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 2 cut(s) 581, 941
PflMI CCANNNNNTGG 1 cut(s) 686
PkrI GCNGC 2 cut(s) 1315, 1324
PleI GAGTC 4 cut(s) 646, 924, 1142, 1243
PpsI GAGTC 4 cut(s) 646, 924, 1142, 1243
Psp1406I AACGTT 1 cut(s) 216
Psp6I CCWGG 2 cut(s) 954, 1105
PspGI CCWGG 2 cut(s) 954, 1105
PspPI GGNCC 3 cut(s) 259, 952, 1124
PsrI GAACNNNNNNTAC 2 cut(s) 1236, 1268
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 2 cut(s) 342, 1303
RsaI GTAC 4 cut(s) 14, 212, 876, 1001
RsaNI GTAC 4 cut(s) 13, 211, 875, 1000
RseI CAYNNNNRTG 2 cut(s) 279, 576
SaqAI TTAA 5 cut(s) 138, 297, 303, 722, 1100
SatI GCNGC 2 cut(s) 1314, 1323
Sau3AI GATC 7 cut(s) 342, 457, 662, 850, 1216, 1291, 1303
Sau96I GGNCC 3 cut(s) 259, 952, 1124
SchI GAGTC 4 cut(s) 647, 925, 1142, 1244
ScrFI CCNGG 2 cut(s) 956, 1107
SduI GDGCHC 2 cut(s) 399, 418
SfaNI GCATC 1 cut(s) 134
SfcI CTRYAG 1 cut(s) 1128
SinI GGWCC 3 cut(s) 259, 952, 1124
SmiMI CAYNNNNRTG 2 cut(s) 279, 576
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
Sse9I AATT 6 cut(s) 191, 298, 776, 882, 1046, 1097
SsiI CCGC 2 cut(s) 252, 825
SspMI CTAG 5 cut(s) 201, 479, 527, 590, 1256
StyD4I CCNGG 2 cut(s) 954, 1105
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 2 cut(s) 1193, 1243
TaiI ACGT 4 cut(s) 21, 219, 652, 721
TaqI TCGA 2 cut(s) 128, 515
TaqII GACCGA 2 cut(s) 940, 1094
TasI AATT 6 cut(s) 191, 298, 776, 882, 1046, 1097
TatI WGTACW 1 cut(s) 210
TfiI GAWTC 2 cut(s) 581, 941
Tru1I TTAA 5 cut(s) 138, 297, 303, 722, 1100
Tru9I TTAA 5 cut(s) 138, 297, 303, 722, 1100
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 2 cut(s) 283, 805
TseI GCWGC 2 cut(s) 1313, 1322
Tsp45I GTSAC 2 cut(s) 283, 805
TspDTI ATGAA 9 cut(s) 21, 226, 418, 486, 560, 638, 1039, 1149, 1197
TspGWI ACGGA 1 cut(s) 251
TspRI CASTG 1 cut(s) 64
Van91I CCANNNNNTGG 1 cut(s) 686
VneI GTGCAC 1 cut(s) 414
VpaK11BI GGWCC 3 cut(s) 259, 952, 1124
XapI RAATTY 2 cut(s) 776, 1046
XceI RCATGY 1 cut(s) 751
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XmnI GAANNNNTTC 1 cut(s) 51
XspI CTAG 5 cut(s) 201, 479, 527, 590, 1256
Zsp2I ATGCAT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.