MD10G1169100.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008200
Physical Location & Seq
Forward (+)
26125853 .. 26130991
5139 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1169100.v1.1.491

Sequence Viewer

Length: 1356 bp
ATGAGAGCTTGCTCTAAACCAACAATGGCGGTCTCTTTGAACTTGTTTTTGTTACTTTTGACAATGGCGGTTCTTCTAGCTCTGGTTGCAAGCACAAATGCCTCTTCAAAGAGTGACAAGAATGTAAATTCTAGGTTTGTGGGACCAAAGGAGTCATTGAGCTCCAAGAACTCATCAATGGCAGAAAGGTCTGATGAAGCTTTGGATGAGCACGCAGTAGATAATCCAGATGAAATAGCCTCCATGGTTGACATGACCATTCGTAATAGCACAGAGAGAAGAAATTTGGGATTCTTTTCATGTGCGACGGGGAATCCAATTGATGATTGTTGGAGATGTGACCCTCAGTGGCAGCTCCACCGCAAGCGCCTCGCAAACTGCGGCATAGGGTTCGGGCGCAATGCCGTGGGTGGCCGCGACGGGAGATACTACGTCGTGAACGACCCCGGCAATGATGATCCCGTCAACCCTAGACCCGGAACCCTCCGCCACGCCGTTATCCAGGACAGGCCGTTGTGGATTGTGTTCAAGCGTGACATGGTGATCACACTAAAGCAAGAGCTAATTATGAACAGCTTCAAGACAATTGATGCACGTGGAGTCAATGTGCACATTGCATATGGGGCATGCATTACGATTCAATATATTACTAATGTCATCATCCATGGGCTACATATCCATGACTGCAAATCTACCGGAAATGCCCTGGTCCGGAGCTCGCCGAGCCATTACGGGTGGAGGACGATGGCGGACGGGGATGGCATTTCCATTTTTGGATCTAGCCACATTTGGATTGACCACAATTCCCTTTCTAATTGTGCCGATGGGCTCATTGATGCTATTATGGGCTCTACTGCAATTACCATTTCCAACAACTACTTCACCCATCATAATGAGGTTATGTTACTGGGTCACAGTGACTCCTATGTAAGGGACAAGCTTATGCAAGTGACAATTGCCTACAATCATTTTGGAGAGGGACTTATTCAAAGGATGCCAAGGTGTAGGCACGGGTATTTCCATGTGGTGAACAATGACTACACACATTGGGAAATGTATGCCATTGGTGGAAGTGCTGAACCCACAATTAACAGCCAGGGCAACAGATATCTGGCCCCTAACAATGCTTTTGCAAAGGAGGTCACACATAGAGTTGATCCAAATGACTGGACGCACTGGAACTGGAGATCAGAAGGAGACCTGATGCTCAATGGAGCCTATTTCATTGCATCAGGAACTGGTTCCGGAGCAAGCTATGCCAGGGCCTCTAGCTTGGGAGCCAAGTCATCTTCCCTGGTTGGCTCCATCACGGCCAGCGCCGGTGTCCTCAGTTGCCGGAGGGGTTACCAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

452

Amino Acids

49.75

Weight (kDa)

7.65

Isoelectric Point (pI)

32.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 194 - 394 3.9e-18 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 417
AccIII TCCGGA 2 cut(s) 711, 1244
AciI CCGC 7 cut(s) 29, 68, 361, 381, 415, 487, 749
AclWI GGATC 3 cut(s) 452, 784, 1151
AcoI YGGCCR 2 cut(s) 412, 1311
AcsI RAATTY 2 cut(s) 127, 283
AcvI CACGTG 1 cut(s) 596
AfiI CCNNNNNNNGG 3 cut(s) 476, 711, 930
AgsI TTSAA 6 cut(s) 40, 108, 529, 580, 641, 989
AjnI CCWGG 5 cut(s) 501, 705, 1095, 1259, 1293
AjuI GAANNNNNNNTTGG 2 cut(s) 863, 895
Alw21I GWGCWC 4 cut(s) 164, 213, 612, 719
Alw26I GTCTC 2 cut(s) 37, 1191
Alw44I GTGCAC 1 cut(s) 608
AlwI GGATC 3 cut(s) 452, 784, 1151
AlwNI CAGNNNCTG 1 cut(s) 1238
Aor13HI TCCGGA 2 cut(s) 711, 1244
AoxI GGCC 5 cut(s) 412, 509, 1113, 1263, 1311
ApaLI GTGCAC 1 cut(s) 608
ApeKI GCWGC 1 cut(s) 352
ApoI RAATTY 2 cut(s) 127, 283
Asp700I GAANNNNTTC 2 cut(s) 575, 1240
AspLEI GCGC 3 cut(s) 369, 399, 1319
AspS9I GGNCC 4 cut(s) 143, 709, 1114, 1263
AsuC2I CCSGG 2 cut(s) 447, 477
AsuHPI GGTGA 3 cut(s) 553, 874, 1039
AvaII GGWCC 2 cut(s) 143, 709
BaeGI GKGCMC 1 cut(s) 612
BanII GRGCYC 4 cut(s) 164, 719, 831, 851
BbrPI CACGTG 1 cut(s) 596
Bbv12I GWGCWC 4 cut(s) 164, 213, 612, 719
BbvI GCAGC 1 cut(s) 364
BccI CCATC 5 cut(s) 739, 752, 818, 894, 1313
BceAI ACGGC 4 cut(s) 389, 479, 496, 1326
BcgI CGANNNNNNTGC 4 cut(s) 352, 373, 386, 407
BciT130I CCWGG 5 cut(s) 503, 707, 1097, 1261, 1295
BclI TGATCA 1 cut(s) 543
BcnI CCSGG 2 cut(s) 447, 477
BcoDI GTCTC 2 cut(s) 37, 1191
BfaI CTAG 5 cut(s) 77, 132, 471, 780, 1269
BfoI RGCGCY 2 cut(s) 370, 1320
BisI GCNGC 3 cut(s) 353, 382, 415
BlsI GCNGC 3 cut(s) 354, 383, 416
Bme1390I CCNGG 7 cut(s) 447, 477, 503, 707, 1097, 1261, 1295
Bme18I GGWCC 2 cut(s) 143, 709
BmgT120I GGNCC 4 cut(s) 143, 709, 1114, 1263
BmiI GGNNCC 7 cut(s) 144, 481, 1116, 1216, 1243, 1279, 1303
BmrFI CCNGG 7 cut(s) 447, 477, 503, 707, 1097, 1261, 1295
BmrI ACTGGG 1 cut(s) 917
BmsI GCATC 5 cut(s) 580, 826, 984, 1194, 1238
BmuI ACTGGG 1 cut(s) 917
BpmI CTGGAG 1 cut(s) 1204
BpuMI CCSGG 2 cut(s) 447, 477
BsaAI YACGTR 1 cut(s) 596
BsaI GGTCTC 2 cut(s) 37, 1191
BsaJI CCNNGG 9 cut(s) 243, 405, 445, 664, 705, 998, 1096, 1260, 1293
BsaWI WCCGGW 3 cut(s) 695, 711, 1244
BsaXI ACNNNNNCTCC 4 cut(s) 591, 621, 905, 935
Bsc4I CCNNNNNNNGG 3 cut(s) 476, 711, 930
Bse118I RCCGGY 1 cut(s) 1319
Bse1I ACTGG 5 cut(s) 912, 1172, 1181, 1187, 1243
Bse3DI GCAATG 4 cut(s) 406, 457, 612, 1224
BseAI TCCGGA 2 cut(s) 711, 1244
BseBI CCWGG 5 cut(s) 503, 707, 1097, 1261, 1295
BseDI CCNNGG 9 cut(s) 243, 405, 445, 664, 705, 998, 1096, 1260, 1293
BseGI GGATG 4 cut(s) 211, 660, 763, 999
BseLI CCNNNNNNNGG 3 cut(s) 476, 711, 930
BseMI GCAATG 4 cut(s) 406, 457, 612, 1224
BseMII CTCAG 2 cut(s) 359, 1342
BseNI ACTGG 5 cut(s) 912, 1172, 1181, 1187, 1243
BseSI GKGCMC 1 cut(s) 612
BseXI GCAGC 1 cut(s) 364
Bsh1236I CGCG 1 cut(s) 417
BshFI GGCC 5 cut(s) 414, 511, 1115, 1265, 1313
BsiHKAI GWGCWC 4 cut(s) 164, 213, 612, 719
BsiSI CCGG 7 cut(s) 447, 477, 696, 712, 1245, 1320, 1336
BslFI GGGAC 3 cut(s) 156, 947, 993
BslI CCNNNNNNNGG 3 cut(s) 476, 711, 930
BsmAI GTCTC 2 cut(s) 37, 1191
BsmFI GGGAC 3 cut(s) 156, 947, 993
BsnI GGCC 5 cut(s) 414, 511, 1115, 1265, 1313
Bso31I GGTCTC 2 cut(s) 37, 1191
Bsp1286I GDGCHC 6 cut(s) 164, 213, 612, 719, 831, 851
Bsp13I TCCGGA 2 cut(s) 711, 1244
Bsp143I GATC 5 cut(s) 457, 543, 776, 1156, 1187
Bsp19I CCATGG 2 cut(s) 243, 664
BspACI CCGC 7 cut(s) 29, 68, 361, 381, 415, 487, 749
BspANI GGCC 5 cut(s) 414, 511, 1115, 1265, 1313
BspCNI CTCAG 2 cut(s) 358, 1341
BspEI TCCGGA 2 cut(s) 711, 1244
BspFNI CGCG 1 cut(s) 417
BspLI GGNNCC 7 cut(s) 144, 481, 1116, 1216, 1243, 1279, 1303
BspPI GGATC 3 cut(s) 452, 784, 1151
BspTNI GGTCTC 2 cut(s) 37, 1191
BsrDI GCAATG 4 cut(s) 406, 457, 612, 1224
BsrFI RCCGGY 1 cut(s) 1319
BsrI ACTGG 5 cut(s) 912, 1172, 1181, 1187, 1243
BssAI RCCGGY 1 cut(s) 1319
BssECI CCNNGG 9 cut(s) 243, 405, 445, 664, 705, 998, 1096, 1260, 1293
BssMI GATC 5 cut(s) 457, 543, 776, 1156, 1187
BssT1I CCWWGG 3 cut(s) 243, 664, 998
Bst2UI CCWGG 5 cut(s) 503, 707, 1097, 1261, 1295
Bst4CI ACNGT 1 cut(s) 917
Bst6I CTCTTC 1 cut(s) 109
BstAPI GCANNNNNTGC 1 cut(s) 1256
BstBAI YACGTR 1 cut(s) 596
BstC8I GCNNGC 8 cut(s) 10, 91, 213, 365, 628, 719, 1252, 1315
BstDEI CTNAG 2 cut(s) 345, 1328
BstDSI CCRYGG 3 cut(s) 243, 405, 664
BstEII GGTNACC 1 cut(s) 1343
BstENI CCTNNNNNAGG 1 cut(s) 928
BstF5I GGATG 4 cut(s) 211, 660, 763, 999
BstFNI CGCG 1 cut(s) 417
BstH2I RGCGCY 2 cut(s) 370, 1320
BstHHI GCGC 3 cut(s) 369, 399, 1319
BstKTI GATC 5 cut(s) 460, 546, 779, 1159, 1190
BstMAI GTCTC 2 cut(s) 37, 1191
BstMBI GATC 5 cut(s) 457, 543, 776, 1156, 1187
BstMWI GCNNNNNNNGC 4 cut(s) 86, 623, 723, 1256
BstNI CCWGG 5 cut(s) 503, 707, 1097, 1261, 1295
BstNSI RCATGY 1 cut(s) 630
BstPI GGTNACC 1 cut(s) 1343
BstSCI CCNGG 7 cut(s) 445, 475, 501, 705, 1095, 1259, 1293
BstSLI GKGCMC 1 cut(s) 612
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 1 cut(s) 364
BstX2I RGATCY 1 cut(s) 776
BstXI CCANNNNNNTGG 1 cut(s) 1167
BstYI RGATCY 1 cut(s) 776
BsuRI GGCC 5 cut(s) 414, 511, 1115, 1265, 1313
BtgI CCRYGG 3 cut(s) 243, 405, 664
BtsCI GGATG 4 cut(s) 211, 660, 763, 999
BtsIMutI CAGTG 3 cut(s) 353, 922, 1174
Cac8I GCNNGC 8 cut(s) 10, 91, 213, 365, 628, 719, 1252, 1315
CaiI CAGNNNCTG 1 cut(s) 1238
CfoI GCGC 3 cut(s) 369, 399, 1319
Cfr10I RCCGGY 1 cut(s) 1319
Cfr13I GGNCC 4 cut(s) 143, 709, 1114, 1263
CseI GACGC 1 cut(s) 1180
CviAII CATG 8 cut(s) 244, 253, 300, 538, 627, 665, 680, 1022
DdeI CTNAG 2 cut(s) 345, 1328
DpnI GATC 5 cut(s) 459, 545, 778, 1158, 1189
DpnII GATC 5 cut(s) 457, 543, 776, 1156, 1187
EaeI YGGCCR 2 cut(s) 412, 1311
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
EciI GGCGGA 2 cut(s) 476, 764
Ecl136II GAGCTC 2 cut(s) 162, 717
Eco130I CCWWGG 3 cut(s) 243, 664, 998
Eco24I GRGCYC 4 cut(s) 164, 719, 831, 851
Eco31I GGTCTC 2 cut(s) 37, 1191
Eco32I GATATC 1 cut(s) 1109
Eco47I GGWCC 2 cut(s) 143, 709
Eco53kI GAGCTC 2 cut(s) 162, 717
Eco72I CACGTG 1 cut(s) 596
Eco91I GGTNACC 1 cut(s) 1343
EcoICRI GAGCTC 2 cut(s) 162, 717
EcoNI CCTNNNNNAGG 1 cut(s) 928
EcoO109I RGGNCCY 1 cut(s) 1263
EcoO65I GGTNACC 1 cut(s) 1343
EcoRII CCWGG 5 cut(s) 501, 705, 1095, 1259, 1293
EcoRV GATATC 1 cut(s) 1109
EcoT14I CCWWGG 3 cut(s) 243, 664, 998
EcoT22I ATGCAT 1 cut(s) 632
EcoT38I GRGCYC 4 cut(s) 164, 719, 831, 851
ErhI CCWWGG 3 cut(s) 243, 664, 998
FaeI CATG 8 cut(s) 247, 256, 303, 541, 630, 668, 683, 1025
FaqI GGGAC 3 cut(s) 156, 947, 993
FatI CATG 8 cut(s) 243, 252, 299, 537, 626, 664, 679, 1021
FauNDI CATATG 1 cut(s) 619
FbaI TGATCA 1 cut(s) 543
Fnu4HI GCNGC 3 cut(s) 353, 382, 415
FokI GGATG 4 cut(s) 218, 647, 770, 1006
FriOI GRGCYC 4 cut(s) 164, 719, 831, 851
Fsp4HI GCNGC 3 cut(s) 353, 382, 415
FspBI CTAG 5 cut(s) 77, 132, 471, 780, 1269
GlaI GCGC 3 cut(s) 368, 398, 1318
GluI GCNGC 3 cut(s) 353, 382, 415
GsuI CTGGAG 1 cut(s) 1204
HaeII RGCGCY 2 cut(s) 370, 1320
HaeIII GGCC 5 cut(s) 414, 511, 1115, 1265, 1313
HapII CCGG 7 cut(s) 447, 477, 696, 712, 1245, 1320, 1336
HgaI GACGC 1 cut(s) 1180
HhaI GCGC 3 cut(s) 369, 399, 1319
Hin1II CATG 8 cut(s) 247, 256, 303, 541, 630, 668, 683, 1025
Hin6I GCGC 3 cut(s) 367, 397, 1317
HinP1I GCGC 3 cut(s) 367, 397, 1317
HincII GTYRAC 2 cut(s) 250, 466
HindII GTYRAC 2 cut(s) 250, 466
HindIII AAGCTT 2 cut(s) 198, 938
HinfI GANTC 6 cut(s) 152, 291, 313, 600, 637, 920
HpaII CCGG 7 cut(s) 447, 477, 696, 712, 1245, 1320, 1336
HphI GGTGA 3 cut(s) 553, 874, 1039
Hpy166II GTNNAC 5 cut(s) 250, 439, 466, 610, 1030
Hpy188I TCNGA 2 cut(s) 193, 1192
Hpy188III TCNNGA 6 cut(s) 227, 436, 580, 712, 1233, 1245
Hpy8I GTNNAC 5 cut(s) 250, 439, 466, 610, 1030
Hpy99I CGWCG 3 cut(s) 310, 422, 437
HpyAV CCTTC 1 cut(s) 1187
HpyCH4III ACNGT 1 cut(s) 917
HpyCH4IV ACGT 2 cut(s) 432, 595
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 623, 723, 1256
HpyF3I CTNAG 2 cut(s) 345, 1328
HpySE526I ACGT 2 cut(s) 432, 595
Hsp92II CATG 8 cut(s) 247, 256, 303, 541, 630, 668, 683, 1025
HspAI GCGC 3 cut(s) 367, 397, 1317
Kpn2I TCCGGA 2 cut(s) 711, 1244
Ksp22I TGATCA 1 cut(s) 543
Kzo9I GATC 5 cut(s) 457, 543, 776, 1156, 1187
LmnI GCTCC 7 cut(s) 167, 360, 714, 1214, 1247, 1277, 1307
Lsp1109I GCAGC 1 cut(s) 364
LweI GCATC 5 cut(s) 580, 826, 984, 1194, 1238
MaeI CTAG 5 cut(s) 77, 132, 471, 780, 1269
MaeII ACGT 2 cut(s) 432, 595
MalI GATC 5 cut(s) 459, 545, 778, 1158, 1189
MboI GATC 5 cut(s) 457, 543, 776, 1156, 1187
MboII GAAGA 4 cut(s) 65, 96, 291, 1281
MfeI CAATTG 3 cut(s) 318, 585, 954
MflI RGATCY 1 cut(s) 776
MhlI GDGCHC 6 cut(s) 164, 213, 612, 719, 831, 851
MlyI GAGTC 3 cut(s) 161, 609, 914
MmeI TCCRAC 2 cut(s) 311, 894
Mph1103I ATGCAT 1 cut(s) 632
MroI TCCGGA 2 cut(s) 711, 1244
MroXI GAANNNNTTC 2 cut(s) 575, 1240
MseI TTAA 2 cut(s) 1089, 1354
MslI CAYNNNNRTG 2 cut(s) 678, 891
MspI CCGG 7 cut(s) 447, 477, 696, 712, 1245, 1320, 1336
MspR9I CCNGG 7 cut(s) 447, 477, 503, 707, 1097, 1261, 1295
MunI CAATTG 3 cut(s) 318, 585, 954
MvaI CCWGG 5 cut(s) 503, 707, 1097, 1261, 1295
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 4 cut(s) 86, 623, 723, 1256
NciI CCSGG 2 cut(s) 447, 477
NcoI CCATGG 2 cut(s) 243, 664
NdeI CATATG 1 cut(s) 619
NdeII GATC 5 cut(s) 457, 543, 776, 1156, 1187
NlaIII CATG 8 cut(s) 247, 256, 303, 541, 630, 668, 683, 1025
NlaIV GGNNCC 7 cut(s) 144, 481, 1116, 1216, 1243, 1279, 1303
NmeAIII GCCGAG 1 cut(s) 747
NmuCI GTSAC 7 cut(s) 113, 338, 533, 911, 917, 949, 1141
NsiI ATGCAT 1 cut(s) 632
NspI RCATGY 1 cut(s) 630
PaeI GCATGC 1 cut(s) 630
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PdmI GAANNNNTTC 2 cut(s) 575, 1240
PfeI GAWTC 3 cut(s) 291, 313, 637
PfoI TCCNGGA 1 cut(s) 501
PkrI GCNGC 3 cut(s) 354, 383, 416
PleI GAGTC 3 cut(s) 160, 608, 914
PmaCI CACGTG 1 cut(s) 596
PmlI CACGTG 1 cut(s) 596
PpsI GAGTC 3 cut(s) 160, 608, 914
Ppu21I YACGTR 1 cut(s) 596
Psp124BI GAGCTC 2 cut(s) 164, 719
Psp6I CCWGG 5 cut(s) 501, 705, 1095, 1259, 1293
PspCI CACGTG 1 cut(s) 596
PspEI GGTNACC 1 cut(s) 1343
PspGI CCWGG 5 cut(s) 501, 705, 1095, 1259, 1293
PspN4I GGNNCC 7 cut(s) 144, 481, 1116, 1216, 1243, 1279, 1303
PspPI GGNCC 4 cut(s) 143, 709, 1114, 1263
PsrI GAACNNNNNNTAC 2 cut(s) 1022, 1054
PstNI CAGNNNCTG 1 cut(s) 1238
PsuI RGATCY 1 cut(s) 776
RseI CAYNNNNRTG 2 cut(s) 678, 891
SacI GAGCTC 2 cut(s) 164, 719
SaqAI TTAA 2 cut(s) 1089, 1354
SatI GCNGC 3 cut(s) 353, 382, 415
Sau3AI GATC 5 cut(s) 457, 543, 776, 1156, 1187
Sau96I GGNCC 4 cut(s) 143, 709, 1114, 1263
SchI GAGTC 3 cut(s) 161, 609, 914
ScrFI CCNGG 7 cut(s) 447, 477, 503, 707, 1097, 1261, 1295
SduI GDGCHC 6 cut(s) 164, 213, 612, 719, 831, 851
SfaNI GCATC 5 cut(s) 580, 826, 984, 1194, 1238
SgrAI CRCCGGYG 1 cut(s) 1319
SinI GGWCC 2 cut(s) 143, 709
SmiMI CAYNNNNRTG 2 cut(s) 678, 891
SphI GCATGC 1 cut(s) 630
SsiI CCGC 7 cut(s) 29, 68, 361, 381, 415, 487, 749
SspMI CTAG 5 cut(s) 77, 132, 471, 780, 1269
SstI GAGCTC 2 cut(s) 164, 719
StyD4I CCNGG 7 cut(s) 445, 475, 501, 705, 1095, 1259, 1293
StyI CCWWGG 3 cut(s) 243, 664, 998
TaaI ACNGT 1 cut(s) 917
TaiI ACGT 2 cut(s) 435, 598
TauI GCSGC 2 cut(s) 384, 417
TfiI GAWTC 3 cut(s) 291, 313, 637
Tru1I TTAA 2 cut(s) 1089, 1354
Tru9I TTAA 2 cut(s) 1089, 1354
TscAI CASTG 3 cut(s) 353, 922, 1181
TseFI GTSAC 7 cut(s) 113, 338, 533, 911, 917, 949, 1141
TseI GCWGC 1 cut(s) 352
Tsp45I GTSAC 7 cut(s) 113, 338, 533, 911, 917, 949, 1141
TspDTI ATGAA 5 cut(s) 210, 246, 288, 584, 1213
TspRI CASTG 3 cut(s) 353, 922, 1181
VneI GTGCAC 1 cut(s) 608
VpaK11BI GGWCC 2 cut(s) 143, 709
XagI CCTNNNNNAGG 1 cut(s) 928
XapI RAATTY 2 cut(s) 127, 283
XceI RCATGY 1 cut(s) 630
XmnI GAANNNNTTC 2 cut(s) 575, 1240
XspI CTAG 5 cut(s) 77, 132, 471, 780, 1269
Zsp2I ATGCAT 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.