Rroxscaffold_7G00183370

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008200
Physical Location & Seq
Reverse (-)
22437184 .. 22441456
4273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00183370.1

Sequence Viewer

Length: 1344 bp
ATGAGATTAGCTAGCTCGAGAGCCACAATGCGGTTCTATATGACCCCACTGCTGCTTCTTTTGGCCTTGCTCGTAAGCGTAGCGGCTTCAGTAGAGAATGACAAGCCTGTACAGTCAAGGTCCGTGGGACTAGAGGAGCCAAAAAGCTCCATCAACTCTTCAATGACAGAAAGATCCAACGATGACTGGAATGAGCACGCAGTAGATAATCCGGAGGAGATCGCATCCTTGGTTGATACGACCATTCGTAACAGTACTGCGAGAAGAAACTTAGGATTTTTCTCATGCGCAACAGGGAATCCCATTGATGACTGCTGGAGATGTGACCCACAATGGCAGCGCCACCGCAAGAGGCTAGCCAATTGCGGCATTGGGTTCGGGCGCAATGCCGTCGGCGGCCGTGACGGAAGGTATTACGTCGTAAATGACCCCGGTCATGATGATCCGGTGAACCCCCGGCCGGGTACCCTCCGTCACGCTGTCATCCAGGACAGGCCTTTGTGGATTGTGTTCAAGCGTGACATGGTGATCACATTGAAGCAGGAGCTTATAATGAACAGCTTCAAGACCATTGACGCTCGTGGAGTCAATGTCCACATTGCTTATGGAGGTTGCATTACTATTCAATACGTCACTAATGTGATCATCCATGGTCTACATATCCATGACTGCAAGCCTACCGGCAATGCCATGGTCCGCAGCTCACCGAGTCATTACGGGTGGAGGACCATGGCTGATGGGGATGGTATATCCATATTCGGAGCCAGCCACATTTGGGTCGACCACAACTCGCTCTCGAATTGCGCCGATGGCCTCATTGATGCTATCATGGGTTCTACTGCCATTACCATTTCTAACAACTACTTCACTCACCATAATGAGGTTATGTTGTTGGGGCACAGTGATTCCTACACAAGGGACAAGCAAATGCAAGTGACCATTGCCTACAATCATTTTGGTGAGGGACTCATCCAAAGAATGCCAAGATGTAGACATGGGTATTTCCATGTGGTGAACAATGACTACACTCACTGGGAAATGTATGCCATCGGTGGAAGTGCTGATCCCACCATTAACAGCCAGGGCAACAGATATCTCGCCCCTACCAACCGTTTTGCTAAAGAGGTGACACATAGAGTTGAGACCACAGGCAGATGGAGGCACTGGAACTGGAGATCAGAAGGAGACCTACTCCTTAATGGAGCGTTTTTCGTCCAATCCGGAGCTGGTGCTGCAGCCAGTTATGCCAGAGCTTCAAGCTTAGGGGCAAAGTCATCTTCCATGATTGGCAGCATTACTGCTGGTGCTGGTGTTCTTAATTGCCGGTCTGGTCGTCAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

447

Amino Acids

49.63

Weight (kDa)

8.64

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 186 - 371 6.5e-18 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 551
Acc16I TGCGCA 1 cut(s) 289
Acc65I GGTACC 1 cut(s) 464
AccB1I GGYRCC 1 cut(s) 464
AccI GTMKAC 3 cut(s) 655, 780, 991
AccIII TCCGGA 2 cut(s) 211, 1220
AciI CCGC 6 cut(s) 31, 83, 346, 366, 396, 697
AclWI GGATC 3 cut(s) 168, 437, 1058
AcoI YGGCCR 2 cut(s) 397, 458
AcuI CTGAAG 1 cut(s) 72
AfaI GTAC 3 cut(s) 111, 256, 466
AfiI CCNNNNNNNGG 6 cut(s) 30, 460, 461, 775, 880, 915
AgsI TTSAA 6 cut(s) 162, 514, 538, 565, 626, 1257
AjnI CCWGG 2 cut(s) 486, 1080
AleI CACNNNNGTG 1 cut(s) 638
AluBI AGCT 9 cut(s) 11, 15, 147, 547, 561, 702, 1226, 1253, 1260
AluI AGCT 9 cut(s) 11, 15, 147, 547, 561, 702, 1226, 1253, 1260
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 2 cut(s) 1136, 1179
AlwI GGATC 3 cut(s) 168, 437, 1058
Ama87I CYCGRG 1 cut(s) 16
Aor13HI TCCGGA 2 cut(s) 211, 1220
AoxI GGCC 5 cut(s) 63, 397, 458, 494, 811
ApeKI GCWGC 6 cut(s) 52, 337, 699, 1232, 1235, 1290
Asp700I GAANNNNTTC 1 cut(s) 560
Asp718I GGTACC 1 cut(s) 464
AspLEI GCGC 4 cut(s) 290, 342, 384, 806
AspS9I GGNCC 3 cut(s) 120, 694, 726
AsuC2I CCSGG 3 cut(s) 432, 457, 462
AsuHPI GGTGA 7 cut(s) 460, 538, 696, 863, 971, 1024, 1138
AsuNHI GCTAGC 2 cut(s) 11, 355
AvaI CYCGRG 1 cut(s) 16
AvaII GGWCC 3 cut(s) 120, 694, 726
BaeGI GKGCMC 1 cut(s) 900
BanI GGYRCC 1 cut(s) 464
BarI GAAGNNNNNNTAC 2 cut(s) 1173, 1205
BauI CACGAG 1 cut(s) 579
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 6 cut(s) 39, 349, 711, 1219, 1247, 1302
BccI CCATC 6 cut(s) 158, 731, 737, 803, 1055, 1149
BceAI ACGGC 2 cut(s) 374, 384
BcgI CGANNNNNNTGC 4 cut(s) 358, 373, 392, 407
BciT130I CCWGG 2 cut(s) 488, 1082
BclI TGATCA 2 cut(s) 528, 642
BcnI CCSGG 3 cut(s) 432, 457, 462
BcoDI GTCTC 2 cut(s) 1136, 1179
BfaI CTAG 3 cut(s) 12, 131, 356
BfmI CTRYAG 1 cut(s) 1233
BfoI RGCGCY 1 cut(s) 343
BisI GCNGC 9 cut(s) 53, 84, 338, 367, 397, 700, 1233, 1236, 1291
BlsI GCNGC 9 cut(s) 54, 85, 339, 368, 398, 701, 1234, 1237, 1292
BmcAI AGTACT 1 cut(s) 256
Bme1390I CCNGG 5 cut(s) 432, 457, 462, 488, 1082
Bme18I GGWCC 3 cut(s) 120, 694, 726
BmeT110I CYCGRG 1 cut(s) 16
BmgT120I GGNCC 3 cut(s) 120, 694, 726
BmiI GGNNCC 3 cut(s) 138, 466, 763
BmrFI CCNGG 5 cut(s) 432, 457, 462, 488, 1082
BmrI ACTGGG 1 cut(s) 1042
BmsI GCATC 2 cut(s) 233, 811
BmtI GCTAGC 2 cut(s) 15, 359
BmuI ACTGGG 1 cut(s) 1042
BoxI GACNNNNGTC 1 cut(s) 432
BplI GAGNNNNNCTC 2 cut(s) 1176, 1208
BpmI CTGGAG 2 cut(s) 337, 1192
Bpu10I CCTNAGC 1 cut(s) 1261
BpuMI CCSGG 3 cut(s) 432, 457, 462
BsaI GGTCTC 2 cut(s) 1136, 1179
BsaJI CCNNGG 8 cut(s) 123, 228, 430, 455, 649, 690, 729, 1081
BsaWI WCCGGW 3 cut(s) 211, 445, 1220
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 6 cut(s) 30, 460, 461, 775, 880, 915
Bse118I RCCGGY 2 cut(s) 680, 1323
Bse1I ACTGG 5 cut(s) 191, 1037, 1169, 1175, 1239
Bse3DI GCAATG 4 cut(s) 391, 597, 691, 939
BseAI TCCGGA 2 cut(s) 211, 1220
BseBI CCWGG 2 cut(s) 488, 1082
BseDI CCNNGG 8 cut(s) 123, 228, 430, 455, 649, 690, 729, 1081
BseGI GGATG 5 cut(s) 224, 483, 645, 748, 969
BseLI CCNNNNNNNGG 6 cut(s) 30, 460, 461, 775, 880, 915
BseMI GCAATG 4 cut(s) 391, 597, 691, 939
BseNI ACTGG 5 cut(s) 191, 1037, 1169, 1175, 1239
BseRI GAGGAG 2 cut(s) 149, 230
BseSI GKGCMC 1 cut(s) 900
BseX3I CGGCCG 2 cut(s) 397, 458
BseXI GCAGC 6 cut(s) 39, 349, 711, 1219, 1247, 1302
Bsh1285I CGRYCG 2 cut(s) 400, 461
BshFI GGCC 5 cut(s) 65, 399, 460, 496, 813
BshNI GGYRCC 1 cut(s) 464
BsiEI CGRYCG 2 cut(s) 400, 461
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 16
BsiSI CCGG 8 cut(s) 212, 432, 446, 457, 461, 681, 1221, 1324
BslFI GGGAC 3 cut(s) 141, 932, 978
BslI CCNNNNNNNGG 6 cut(s) 30, 460, 461, 775, 880, 915
BsmAI GTCTC 2 cut(s) 1136, 1179
BsmFI GGGAC 3 cut(s) 141, 932, 978
BsmI GAATGC 1 cut(s) 984
BsnI GGCC 5 cut(s) 65, 399, 460, 496, 813
Bso31I GGTCTC 2 cut(s) 1136, 1179
BsoBI CYCGRG 1 cut(s) 16
Bsp1286I GDGCHC 2 cut(s) 198, 900
Bsp13I TCCGGA 2 cut(s) 211, 1220
Bsp1407I TGTACA 1 cut(s) 109
Bsp143I GATC 7 cut(s) 173, 219, 442, 528, 642, 1063, 1175
Bsp19I CCATGG 3 cut(s) 649, 690, 729
BspACI CCGC 6 cut(s) 31, 83, 346, 366, 396, 697
BspANI GGCC 5 cut(s) 65, 399, 460, 496, 813
BspEI TCCGGA 2 cut(s) 211, 1220
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 3 cut(s) 138, 466, 763
BspMAI CTGCAG 1 cut(s) 1237
BspOI GCTAGC 2 cut(s) 15, 359
BspPI GGATC 3 cut(s) 168, 437, 1058
BspT107I GGYRCC 1 cut(s) 464
BspTNI GGTCTC 2 cut(s) 1136, 1179
BsrDI GCAATG 4 cut(s) 391, 597, 691, 939
BsrFI RCCGGY 2 cut(s) 680, 1323
BsrGI TGTACA 1 cut(s) 109
BsrI ACTGG 5 cut(s) 191, 1037, 1169, 1175, 1239
BssAI RCCGGY 2 cut(s) 680, 1323
BssECI CCNNGG 8 cut(s) 123, 228, 430, 455, 649, 690, 729, 1081
BssMI GATC 7 cut(s) 173, 219, 442, 528, 642, 1063, 1175
BssSI CACGAG 1 cut(s) 579
BssT1I CCWWGG 4 cut(s) 228, 649, 690, 729
Bst2BI CACGAG 1 cut(s) 579
Bst2UI CCWGG 2 cut(s) 488, 1082
Bst4CI ACNGT 4 cut(s) 114, 254, 902, 1112
Bst6I CTCTTC 1 cut(s) 163
BstAUI TGTACA 1 cut(s) 109
BstC8I GCNNGC 5 cut(s) 13, 198, 357, 674, 766
BstDEI CTNAG 2 cut(s) 271, 1261
BstDSI CCRYGG 4 cut(s) 123, 649, 690, 729
BstENI CCTNNNNNAGG 1 cut(s) 913
BstF5I GGATG 5 cut(s) 224, 483, 645, 748, 969
BstH2I RGCGCY 1 cut(s) 343
BstHHI GCGC 4 cut(s) 290, 342, 384, 806
BstKTI GATC 7 cut(s) 176, 222, 445, 531, 645, 1066, 1178
BstMAI GTCTC 2 cut(s) 1136, 1179
BstMBI GATC 7 cut(s) 173, 219, 442, 528, 642, 1063, 1175
BstMCI CGRYCG 2 cut(s) 400, 461
BstMWI GCNNNNNNNGC 3 cut(s) 810, 1232, 1244
BstNI CCWGG 2 cut(s) 488, 1082
BstPAI GACNNNNGTC 1 cut(s) 432
BstSCI CCNGG 5 cut(s) 430, 455, 460, 486, 1080
BstSFI CTRYAG 1 cut(s) 1233
BstSLI GKGCMC 1 cut(s) 900
BstV1I GCAGC 6 cut(s) 39, 349, 711, 1219, 1247, 1302
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
BstZI CGGCCG 2 cut(s) 397, 458
BsuRI GGCC 5 cut(s) 65, 399, 460, 496, 813
BtgI CCRYGG 4 cut(s) 123, 649, 690, 729
BtsCI GGATG 5 cut(s) 224, 483, 645, 748, 969
BtsI GCAGTG 1 cut(s) 47
BtsIMutI CAGTG 4 cut(s) 47, 907, 1030, 1162
Cac8I GCNNGC 5 cut(s) 13, 198, 357, 674, 766
CciI TCATGA 1 cut(s) 436
CfoI GCGC 4 cut(s) 290, 342, 384, 806
Cfr10I RCCGGY 2 cut(s) 680, 1323
Cfr13I GGNCC 3 cut(s) 120, 694, 726
CseI GACGC 1 cut(s) 584
Csp6I GTAC 3 cut(s) 110, 255, 465
CspCI CAANNNNNGTGG 2 cut(s) 105, 140
CviQI GTAC 3 cut(s) 110, 255, 465
DdeI CTNAG 2 cut(s) 271, 1261
DpnI GATC 7 cut(s) 175, 221, 444, 530, 644, 1065, 1177
DpnII GATC 7 cut(s) 173, 219, 442, 528, 642, 1063, 1175
EaeI YGGCCR 2 cut(s) 397, 458
EagI CGGCCG 2 cut(s) 397, 458
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
EclXI CGGCCG 2 cut(s) 397, 458
Eco130I CCWWGG 4 cut(s) 228, 649, 690, 729
Eco147I AGGCCT 1 cut(s) 496
Eco31I GGTCTC 2 cut(s) 1136, 1179
Eco32I GATATC 1 cut(s) 1094
Eco47I GGWCC 3 cut(s) 120, 694, 726
Eco52I CGGCCG 2 cut(s) 397, 458
Eco57I CTGAAG 1 cut(s) 72
Eco88I CYCGRG 1 cut(s) 16
EcoNI CCTNNNNNAGG 1 cut(s) 913
EcoRII CCWGG 2 cut(s) 486, 1080
EcoRV GATATC 1 cut(s) 1094
EcoT14I CCWWGG 4 cut(s) 228, 649, 690, 729
ErhI CCWWGG 4 cut(s) 228, 649, 690, 729
FaqI GGGAC 3 cut(s) 141, 932, 978
FbaI TGATCA 2 cut(s) 528, 642
FblI GTMKAC 3 cut(s) 655, 780, 991
Fnu4HI GCNGC 9 cut(s) 53, 84, 338, 367, 397, 700, 1233, 1236, 1291
FokI GGATG 5 cut(s) 211, 470, 632, 755, 956
Fsp4HI GCNGC 9 cut(s) 53, 84, 338, 367, 397, 700, 1233, 1236, 1291
FspBI CTAG 3 cut(s) 12, 131, 356
FspI TGCGCA 1 cut(s) 289
GlaI GCGC 4 cut(s) 289, 341, 383, 805
GluI GCNGC 9 cut(s) 53, 84, 338, 367, 397, 700, 1233, 1236, 1291
GsuI CTGGAG 2 cut(s) 337, 1192
HaeII RGCGCY 1 cut(s) 343
HaeIII GGCC 5 cut(s) 65, 399, 460, 496, 813
HapII CCGG 8 cut(s) 212, 432, 446, 457, 461, 681, 1221, 1324
HgaI GACGC 1 cut(s) 584
HhaI GCGC 4 cut(s) 290, 342, 384, 806
Hin6I GCGC 4 cut(s) 288, 340, 382, 804
HinP1I GCGC 4 cut(s) 288, 340, 382, 804
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 1258
HinfI GANTC 5 cut(s) 298, 585, 709, 905, 966
HpaII CCGG 8 cut(s) 212, 432, 446, 457, 461, 681, 1221, 1324
HphI GGTGA 7 cut(s) 460, 538, 696, 863, 971, 1024, 1138
Hpy166II GTNNAC 6 cut(s) 451, 595, 656, 781, 992, 1015
Hpy188I TCNGA 2 cut(s) 761, 1180
Hpy188III TCNNGA 6 cut(s) 18, 212, 437, 565, 796, 1221
Hpy8I GTNNAC 6 cut(s) 451, 595, 656, 781, 992, 1015
Hpy99I CGWCG 2 cut(s) 395, 422
HpyAV CCTTC 2 cut(s) 402, 1175
HpyCH4III ACNGT 4 cut(s) 114, 254, 902, 1112
HpyCH4IV ACGT 2 cut(s) 417, 630
HpyCH4V TGCA 4 cut(s) 615, 672, 931, 1235
HpyF10VI GCNNNNNNNGC 3 cut(s) 810, 1232, 1244
HpyF3I CTNAG 2 cut(s) 271, 1261
HpySE526I ACGT 2 cut(s) 417, 630
HspAI GCGC 4 cut(s) 288, 340, 382, 804
Kpn2I TCCGGA 2 cut(s) 211, 1220
KpnI GGTACC 1 cut(s) 468
Ksp22I TGATCA 2 cut(s) 528, 642
Kzo9I GATC 7 cut(s) 173, 219, 442, 528, 642, 1063, 1175
LmnI GCTCC 6 cut(s) 136, 152, 544, 761, 1202, 1223
Lsp1109I GCAGC 6 cut(s) 39, 349, 711, 1219, 1247, 1302
LweI GCATC 2 cut(s) 233, 811
MaeI CTAG 3 cut(s) 12, 131, 356
MaeII ACGT 2 cut(s) 417, 630
MaeIII GTNAC 8 cut(s) 248, 323, 401, 473, 518, 631, 934, 1126
MalI GATC 7 cut(s) 175, 221, 444, 530, 644, 1065, 1177
MboI GATC 7 cut(s) 173, 219, 442, 528, 642, 1063, 1175
MboII GAAGA 3 cut(s) 150, 276, 1269
MfeI CAATTG 1 cut(s) 361
MflI RGATCY 1 cut(s) 173
MhlI GDGCHC 2 cut(s) 198, 900
MluCI AATT 3 cut(s) 361, 799, 1318
MlyI GAGTC 3 cut(s) 594, 718, 960
MmeI TCCRAC 1 cut(s) 201
MroI TCCGGA 2 cut(s) 211, 1220
MroXI GAANNNNTTC 1 cut(s) 560
MseI TTAA 4 cut(s) 1074, 1197, 1317, 1342
MslI CAYNNNNRTG 4 cut(s) 638, 663, 876, 957
MspI CCGG 8 cut(s) 212, 432, 446, 457, 461, 681, 1221, 1324
MspR9I CCNGG 5 cut(s) 432, 457, 462, 488, 1082
MunI CAATTG 1 cut(s) 361
Mva1269I GAATGC 1 cut(s) 984
MvaI CCWGG 2 cut(s) 488, 1082
MwoI GCNNNNNNNGC 3 cut(s) 810, 1232, 1244
NciI CCSGG 3 cut(s) 432, 457, 462
NcoI CCATGG 3 cut(s) 649, 690, 729
NdeII GATC 7 cut(s) 173, 219, 442, 528, 642, 1063, 1175
NheI GCTAGC 2 cut(s) 11, 355
NlaIV GGNNCC 3 cut(s) 138, 466, 763
NmuCI GTSAC 7 cut(s) 323, 401, 473, 518, 631, 934, 1126
NsbI TGCGCA 1 cut(s) 289
OliI CACNNNNGTG 1 cut(s) 638
PaeR7I CTCGAG 1 cut(s) 16
PagI TCATGA 1 cut(s) 436
PceI AGGCCT 1 cut(s) 496
PctI GAATGC 1 cut(s) 984
PdmI GAANNNNTTC 1 cut(s) 560
PfeI GAWTC 2 cut(s) 298, 905
PfoI TCCNGGA 1 cut(s) 486
PkrI GCNGC 9 cut(s) 54, 85, 339, 368, 398, 701, 1234, 1237, 1292
PleI GAGTC 3 cut(s) 593, 717, 960
PpsI GAGTC 3 cut(s) 593, 717, 960
PshAI GACNNNNGTC 1 cut(s) 432
PsiI TTATAA 1 cut(s) 551
Psp6I CCWGG 2 cut(s) 486, 1080
PspGI CCWGG 2 cut(s) 486, 1080
PspN4I GGNNCC 3 cut(s) 138, 466, 763
PspPI GGNCC 3 cut(s) 120, 694, 726
PsrI GAACNNNNNNTAC 2 cut(s) 1007, 1039
PstI CTGCAG 1 cut(s) 1237
PsuI RGATCY 1 cut(s) 173
RsaI GTAC 3 cut(s) 111, 256, 466
RsaNI GTAC 3 cut(s) 110, 255, 465
RseI CAYNNNNRTG 4 cut(s) 638, 663, 876, 957
SalI GTCGAC 1 cut(s) 779
SaqAI TTAA 4 cut(s) 1074, 1197, 1317, 1342
SatI GCNGC 9 cut(s) 53, 84, 338, 367, 397, 700, 1233, 1236, 1291
Sau3AI GATC 7 cut(s) 173, 219, 442, 528, 642, 1063, 1175
Sau96I GGNCC 3 cut(s) 120, 694, 726
ScaI AGTACT 1 cut(s) 256
SchI GAGTC 3 cut(s) 594, 718, 960
ScrFI CCNGG 5 cut(s) 432, 457, 462, 488, 1082
SduI GDGCHC 2 cut(s) 198, 900
SfaNI GCATC 2 cut(s) 233, 811
SfcI CTRYAG 1 cut(s) 1233
Sfr274I CTCGAG 1 cut(s) 16
SinI GGWCC 3 cut(s) 120, 694, 726
SlaI CTCGAG 1 cut(s) 16
SmiMI CAYNNNNRTG 4 cut(s) 638, 663, 876, 957
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
Sse9I AATT 3 cut(s) 361, 799, 1318
SseBI AGGCCT 1 cut(s) 496
SsiI CCGC 6 cut(s) 31, 83, 346, 366, 396, 697
SspMI CTAG 3 cut(s) 12, 131, 356
StuI AGGCCT 1 cut(s) 496
StyD4I CCNGG 5 cut(s) 430, 455, 460, 486, 1080
StyI CCWWGG 4 cut(s) 228, 649, 690, 729
TaaI ACNGT 4 cut(s) 114, 254, 902, 1112
TaiI ACGT 2 cut(s) 420, 633
TaqI TCGA 3 cut(s) 17, 780, 797
TasI AATT 3 cut(s) 361, 799, 1318
TatI WGTACW 2 cut(s) 109, 254
TauI GCSGC 3 cut(s) 86, 369, 399
TfiI GAWTC 2 cut(s) 298, 905
Tru1I TTAA 4 cut(s) 1074, 1197, 1317, 1342
Tru9I TTAA 4 cut(s) 1074, 1197, 1317, 1342
TscAI CASTG 4 cut(s) 54, 907, 1037, 1169
TseFI GTSAC 7 cut(s) 323, 401, 473, 518, 631, 934, 1126
TseI GCWGC 6 cut(s) 52, 337, 699, 1232, 1235, 1290
Tsp45I GTSAC 7 cut(s) 323, 401, 473, 518, 631, 934, 1126
TspDTI ATGAA 1 cut(s) 569
TspGWI ACGGA 3 cut(s) 112, 420, 461
TspRI CASTG 4 cut(s) 54, 907, 1037, 1169
VpaK11BI GGWCC 3 cut(s) 120, 694, 726
XagI CCTNNNNNAGG 1 cut(s) 913
XcmI CCANNNNNNNNNTGG 2 cut(s) 602, 1223
XhoI CTCGAG 1 cut(s) 16
XmiI GTMKAC 3 cut(s) 655, 780, 991
XmnI GAANNNNTTC 1 cut(s) 560
XspI CTAG 3 cut(s) 12, 131, 356
ZrmI AGTACT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.