AT3G07010

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008200
Physical Location & Seq
Reverse (-)
2212289 .. 2216483
4195 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G07010.2

Sequence Viewer

Length: 1251 bp
ATGGCTGTCACAAAACTTATTCTCTTTGCTTCTGCATTACTTCTAACAGCTCTATTTATCGGCGTTAATGCATCCAGGTCCAATGAAACATGGCATGAACATGCAGTTGAGAACCCAGATGAAGTCGCTGCCATGGTGGACATGAGCATTCGAAACAGCACAGAGCGGAGAAGATTAGGTTACTTCTCCTGCGCCACCGGCAACCCAATCGACGATTGCTGGCGTTGCGACCGCAAATGGCAGCTCCGTCGCAAACGTTTAGCCGATTGCTCAATCGGATTTGGCCGCAACGCAATCGGAGGCCGCGACGGTCGTTTCTACGTCGTAACCGACCCTGGAGACGACGATCCCGTTAATCCCATACCGGGAACACTCCGTCACGCCGTGATTCAAGACGAACCACTCTGGATCATCTTCAAACGCGACATGGTTATAACCTTGAAGCAAGAGCTGATCATGAACAGCTTCAAAACCATCGATGGTCGTGGTGTCAATGTTCACATCGCTAATGGAGCTTGTCTCACGATTCAATACGTTACGAACATTATTGTCCATGGGATTCATGTCCATGACTGTAAGCCTACGGGTAACGCTATGGTTAGAAGCTCTCCGTCCCATTATGGGTTTAGATCAATGGCTGATGGTGACGCGATCTCGATTTTTGGATCGAGTCATATCTGGATTGATCACAATTCGCTTTCTAATTGTGCTGATGGACTTGTTGACGCTGTTATGAGCTCCACCGCCATTACTGTCTCAAACAATTTCTTCACCCACCACAATGAGGTTATGTTGTTGGGGCATAGTGATTCATACACAAGAGACAAGGTGATGCAAGTTACAATTGCTTACAACCATTTTGGTGAAGGTTTAATCCAGAGAATGCCAAGATGTAGGCATGGATATTTCCATGTAGTTAACAATGACTACACTCATTGGGAAATGTATGCAATTGGTGGTAGTGCTGGTCCAACCATCAACAGTCAGGGCAATCGATTTCTTGCTCCAGTCAACCCATTTGCTAAGGAGGTGACTAAGAGAGAGTACACAGGAGAATCGAAATGGAAGCACTGGAATTGGAGATCAGAAGGAGATCTTTTCTTAAACGGAGCATTTTTCACACGTTCTGGAGCTGGAGCTGGAGCCAACTATGCCAGAGCTTCGAGTTTATCAGCCAAATCATCCTCACTCGTCGGCACCATGACCTCTTACTCAGGTGCTCTTAACTGCAGAGCCGGTCGTCGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

416

Amino Acids

46.17

Weight (kDa)

8.64

Isoelectric Point (pI)

40.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 117 - 313 3.7e-18 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
AccB1I GGYRCC 1 cut(s) 1196
AccBSI CCGCTC 1 cut(s) 166
AccII CGCG 3 cut(s) 306, 423, 650
AciI CCGC 5 cut(s) 166, 232, 286, 304, 744
AclI AACGTT 1 cut(s) 256
AclWI GGATC 3 cut(s) 341, 416, 673
AcoI YGGCCR 1 cut(s) 283
AdeI CACNNNGTG 1 cut(s) 385
AfaI GTAC 1 cut(s) 1046
AfiI CCNNNNNNNGG 3 cut(s) 365, 621, 784
AflIII ACRYGT 1 cut(s) 1121
AgsI TTSAA 5 cut(s) 392, 418, 442, 469, 530
AjnI CCWGG 2 cut(s) 74, 334
Alw21I GWGCWC 2 cut(s) 740, 1222
Alw26I GTCTC 4 cut(s) 333, 524, 760, 816
AlwI GGATC 3 cut(s) 341, 416, 673
AoxI GGCC 2 cut(s) 283, 301
ApeKI GCWGC 2 cut(s) 128, 241
Asp700I GAANNNNTTC 1 cut(s) 464
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 78, 968
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 5 cut(s) 656, 763, 841, 875, 1042
AsuII TTCGAA 1 cut(s) 151
AvaII GGWCC 2 cut(s) 78, 968
BanI GGYRCC 1 cut(s) 1196
BanII GRGCYC 1 cut(s) 740
Bbv12I GWGCWC 2 cut(s) 740, 1222
BbvI GCAGC 2 cut(s) 115, 253
BccI CCATC 5 cut(s) 473, 482, 635, 707, 983
BceAI ACGGC 1 cut(s) 368
BcgI CGANNNNNNTGC 6 cut(s) 190, 224, 230, 264, 277, 311
BciT130I CCWGG 2 cut(s) 76, 336
BclI TGATCA 2 cut(s) 453, 685
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 4 cut(s) 333, 524, 760, 816
BfmI CTRYAG 1 cut(s) 1228
BglII AGATCT 1 cut(s) 1093
BisI GCNGC 4 cut(s) 129, 242, 286, 304
BlsI GCNGC 4 cut(s) 130, 243, 287, 305
Bme1390I CCNGG 3 cut(s) 76, 336, 366
Bme18I GGWCC 2 cut(s) 78, 968
BmgT120I GGNCC 2 cut(s) 78, 968
BmiI GGNNCC 2 cut(s) 1144, 1198
BmrFI CCNGG 3 cut(s) 76, 336, 366
BmsI GCATC 2 cut(s) 80, 822
BplI GAGNNNNNCTC 2 cut(s) 504, 536
BpmI CTGGAG 5 cut(s) 357, 990, 1149, 1155, 1161
Bpu10I CCTNAGC 1 cut(s) 1023
Bpu14I TTCGAA 1 cut(s) 151
BpuMI CCSGG 1 cut(s) 366
Bsa29I ATCGAT 2 cut(s) 477, 994
BsaJI CCNNGG 3 cut(s) 132, 334, 553
Bsc4I CCNNNNNNNGG 3 cut(s) 365, 621, 784
Bse118I RCCGGY 2 cut(s) 197, 1235
Bse1I ACTGG 2 cut(s) 1007, 1076
BseBI CCWGG 2 cut(s) 76, 336
BseCI ATCGAT 2 cut(s) 477, 994
BseDI CCNNGG 3 cut(s) 132, 334, 553
BseGI GGATG 2 cut(s) 71, 1181
BseLI CCNNNNNNNGG 3 cut(s) 365, 621, 784
BseMII CTCAG 1 cut(s) 1227
BseNI ACTGG 2 cut(s) 1007, 1076
BseXI GCAGC 2 cut(s) 115, 253
Bsh1236I CGCG 3 cut(s) 306, 423, 650
Bsh1285I CGRYCG 3 cut(s) 232, 313, 1240
BshFI GGCC 2 cut(s) 285, 303
BshNI GGYRCC 1 cut(s) 1196
BshVI ATCGAT 2 cut(s) 477, 994
BsiEI CGRYCG 3 cut(s) 232, 313, 1240
BsiHKAI GWGCWC 2 cut(s) 740, 1222
BsiSI CCGG 3 cut(s) 198, 365, 1236
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 3 cut(s) 365, 621, 784
BsmAI GTCTC 4 cut(s) 333, 524, 760, 816
BsmBI CGTCTC 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 598
BsmI GAATGC 2 cut(s) 147, 888
BsnI GGCC 2 cut(s) 285, 303
Bsp119I TTCGAA 1 cut(s) 151
Bsp1286I GDGCHC 2 cut(s) 740, 1222
Bsp143I GATC 9 cut(s) 346, 408, 453, 629, 651, 665, 685, 1082, 1093
Bsp19I CCATGG 2 cut(s) 132, 553
BspACI CCGC 5 cut(s) 166, 232, 286, 304, 744
BspANI GGCC 2 cut(s) 285, 303
BspCNI CTCAG 1 cut(s) 1226
BspDI ATCGAT 2 cut(s) 477, 994
BspFNI CGCG 3 cut(s) 306, 423, 650
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 2 cut(s) 1144, 1198
BspMAI CTGCAG 1 cut(s) 1232
BspPI GGATC 3 cut(s) 341, 416, 673
BspT104I TTCGAA 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 1196
BsrBI CCGCTC 1 cut(s) 166
BsrFI RCCGGY 2 cut(s) 197, 1235
BsrI ACTGG 2 cut(s) 1007, 1076
BssAI RCCGGY 2 cut(s) 197, 1235
BssECI CCNNGG 3 cut(s) 132, 334, 553
BssMI GATC 9 cut(s) 346, 408, 453, 629, 651, 665, 685, 1082, 1093
BssT1I CCWWGG 2 cut(s) 132, 553
Bst2UI CCWGG 2 cut(s) 76, 336
Bst4CI ACNGT 4 cut(s) 311, 575, 754, 983
BstBI TTCGAA 1 cut(s) 151
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 3 cut(s) 1023, 1035, 1213
BstDSI CCRYGG 2 cut(s) 132, 553
BstF5I GGATG 2 cut(s) 71, 1181
BstFNI CGCG 3 cut(s) 306, 423, 650
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 9 cut(s) 349, 411, 456, 632, 654, 668, 688, 1085, 1096
BstMAI GTCTC 4 cut(s) 333, 524, 760, 816
BstMBI GATC 9 cut(s) 346, 408, 453, 629, 651, 665, 685, 1082, 1093
BstMCI CGRYCG 3 cut(s) 232, 313, 1240
BstMWI GCNNNNNNNGC 4 cut(s) 198, 225, 512, 1151
BstNI CCWGG 2 cut(s) 76, 336
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 3 cut(s) 74, 334, 364
BstSFI CTRYAG 1 cut(s) 1228
BstUI CGCG 3 cut(s) 306, 423, 650
BstV1I GCAGC 2 cut(s) 115, 253
BstX2I RGATCY 1 cut(s) 1093
BstYI RGATCY 1 cut(s) 1093
Bsu15I ATCGAT 2 cut(s) 477, 994
BsuRI GGCC 2 cut(s) 285, 303
BsuTUI ATCGAT 2 cut(s) 477, 994
BtgI CCRYGG 2 cut(s) 132, 553
BtgZI GCGATG 1 cut(s) 487
BtsCI GGATG 2 cut(s) 71, 1181
BtsIMutI CAGTG 1 cut(s) 1069
Cac8I GCNNGC 1 cut(s) 221
CciI TCATGA 1 cut(s) 456
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 2 cut(s) 197, 1235
Cfr13I GGNCC 2 cut(s) 78, 968
ClaI ATCGAT 2 cut(s) 477, 994
CseI GACGC 2 cut(s) 656, 734
Csp6I GTAC 1 cut(s) 1045
CviQI GTAC 1 cut(s) 1045
DdeI CTNAG 3 cut(s) 1023, 1035, 1213
DpnI GATC 9 cut(s) 348, 410, 455, 631, 653, 667, 687, 1084, 1095
DpnII GATC 9 cut(s) 346, 408, 453, 629, 651, 665, 685, 1082, 1093
DraIII CACNNNGTG 1 cut(s) 385
EaeI YGGCCR 1 cut(s) 283
Ecl136II GAGCTC 1 cut(s) 738
Eco130I CCWWGG 2 cut(s) 132, 553
Eco24I GRGCYC 1 cut(s) 740
Eco47I GGWCC 2 cut(s) 78, 968
Eco53kI GAGCTC 1 cut(s) 738
EcoICRI GAGCTC 1 cut(s) 738
EcoRII CCWGG 2 cut(s) 74, 334
EcoT14I CCWWGG 2 cut(s) 132, 553
EcoT22I ATGCAT 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 740
ErhI CCWWGG 2 cut(s) 132, 553
Esp3I CGTCTC 1 cut(s) 333
FalI AAGNNNNNCTT 2 cut(s) 1080, 1112
FaqI GGGAC 1 cut(s) 598
FbaI TGATCA 2 cut(s) 453, 685
Fnu4HI GCNGC 4 cut(s) 129, 242, 286, 304
FokI GGATG 2 cut(s) 58, 1168
FriOI GRGCYC 1 cut(s) 740
Fsp4HI GCNGC 4 cut(s) 129, 242, 286, 304
GlaI GCGC 1 cut(s) 193
GluI GCNGC 4 cut(s) 129, 242, 286, 304
GsuI CTGGAG 5 cut(s) 357, 990, 1149, 1155, 1161
HaeIII GGCC 2 cut(s) 285, 303
HapII CCGG 3 cut(s) 198, 365, 1236
HgaI GACGC 2 cut(s) 656, 734
HhaI GCGC 1 cut(s) 194
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HincII GTYRAC 3 cut(s) 724, 919, 1012
HindII GTYRAC 3 cut(s) 724, 919, 1012
HinfI GANTC 6 cut(s) 388, 526, 559, 670, 809, 1055
HpaI GTTAAC 1 cut(s) 919
HpaII CCGG 3 cut(s) 198, 365, 1236
HphI GGTGA 5 cut(s) 656, 763, 841, 875, 1042
Hpy166II GTNNAC 6 cut(s) 139, 499, 724, 919, 1012, 1047
Hpy188I TCNGA 3 cut(s) 278, 299, 1087
Hpy188III TCNNGA 8 cut(s) 392, 406, 457, 523, 655, 679, 877, 1128
Hpy8I GTNNAC 6 cut(s) 139, 499, 724, 919, 1012, 1047
Hpy99I CGWCG 7 cut(s) 215, 252, 311, 326, 347, 1196, 1245
HpyAV CCTTC 2 cut(s) 860, 1082
HpyCH4III ACNGT 4 cut(s) 311, 575, 754, 983
HpyCH4IV ACGT 4 cut(s) 256, 321, 534, 1123
HpyCH4V TGCA 6 cut(s) 35, 71, 104, 835, 950, 1230
HpyF10VI GCNNNNNNNGC 4 cut(s) 198, 225, 512, 1151
HpyF3I CTNAG 3 cut(s) 1023, 1035, 1213
HpySE526I ACGT 4 cut(s) 256, 321, 534, 1123
HspAI GCGC 1 cut(s) 192
Ksp22I TGATCA 2 cut(s) 453, 685
KspAI GTTAAC 1 cut(s) 919
Kzo9I GATC 9 cut(s) 346, 408, 453, 629, 651, 665, 685, 1082, 1093
LmnI GCTCC 8 cut(s) 249, 512, 743, 1009, 1109, 1130, 1136, 1142
Lsp1109I GCAGC 2 cut(s) 115, 253
LweI GCATC 2 cut(s) 80, 822
MaeII ACGT 4 cut(s) 256, 321, 534, 1123
MaeIII GTNAC 9 cut(s) 7, 179, 325, 377, 535, 587, 644, 838, 1030
MalI GATC 9 cut(s) 348, 410, 455, 631, 653, 667, 687, 1084, 1095
MbiI CCGCTC 1 cut(s) 166
MboI GATC 9 cut(s) 346, 408, 453, 629, 651, 665, 685, 1082, 1093
MboII GAAGA 3 cut(s) 183, 406, 760
MfeI CAATTG 2 cut(s) 843, 951
MflI RGATCY 1 cut(s) 1093
MhlI GDGCHC 2 cut(s) 740, 1222
MluCI AATT 6 cut(s) 691, 703, 763, 843, 951, 1075
MlyI GAGTC 1 cut(s) 679
MmeI TCCRAC 1 cut(s) 995
MnlI CCTC 5 cut(s) 293, 778, 1021, 1195, 1216
Mph1103I ATGCAT 1 cut(s) 73
MroXI GAANNNNTTC 1 cut(s) 464
MseI TTAA 7 cut(s) 66, 354, 872, 918, 1103, 1224, 1249
MslI CAYNNNNRTG 4 cut(s) 99, 567, 780, 861
MspI CCGG 3 cut(s) 198, 365, 1236
MspR9I CCNGG 3 cut(s) 76, 336, 366
MunI CAATTG 2 cut(s) 843, 951
Mva1269I GAATGC 2 cut(s) 147, 888
MvaI CCWGG 2 cut(s) 76, 336
MvnI CGCG 3 cut(s) 306, 423, 650
MwoI GCNNNNNNNGC 4 cut(s) 198, 225, 512, 1151
NciI CCSGG 1 cut(s) 366
NcoI CCATGG 2 cut(s) 132, 553
NdeII GATC 9 cut(s) 346, 408, 453, 629, 651, 665, 685, 1082, 1093
NlaIV GGNNCC 2 cut(s) 1144, 1198
NmuCI GTSAC 4 cut(s) 7, 377, 644, 1030
NsiI ATGCAT 1 cut(s) 73
NspI RCATGY 1 cut(s) 104
NspV TTCGAA 1 cut(s) 151
PagI TCATGA 1 cut(s) 456
PcsI WCGNNNNNNNCGW 3 cut(s) 303, 327, 348
PctI GAATGC 2 cut(s) 147, 888
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 5 cut(s) 388, 526, 559, 809, 1055
PkrI GCNGC 4 cut(s) 130, 243, 287, 305
PleI GAGTC 1 cut(s) 678
PpsI GAGTC 1 cut(s) 678
PsiI TTATAA 1 cut(s) 434
Psp124BI GAGCTC 1 cut(s) 740
Psp1406I AACGTT 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 74, 334
PspGI CCWGG 2 cut(s) 74, 334
PspN4I GGNNCC 2 cut(s) 1144, 1198
PspPI GGNCC 2 cut(s) 78, 968
PstI CTGCAG 1 cut(s) 1232
PsuI RGATCY 1 cut(s) 1093
RsaI GTAC 1 cut(s) 1046
RsaNI GTAC 1 cut(s) 1045
RseI CAYNNNNRTG 4 cut(s) 99, 567, 780, 861
SacI GAGCTC 1 cut(s) 740
SaqAI TTAA 7 cut(s) 66, 354, 872, 918, 1103, 1224, 1249
SatI GCNGC 4 cut(s) 129, 242, 286, 304
Sau3AI GATC 9 cut(s) 346, 408, 453, 629, 651, 665, 685, 1082, 1093
Sau96I GGNCC 2 cut(s) 78, 968
SchI GAGTC 1 cut(s) 679
ScrFI CCNGG 3 cut(s) 76, 336, 366
SduI GDGCHC 2 cut(s) 740, 1222
SfaNI GCATC 2 cut(s) 80, 822
SfcI CTRYAG 1 cut(s) 1228
SfuI TTCGAA 1 cut(s) 151
SinI GGWCC 2 cut(s) 78, 968
SmiMI CAYNNNNRTG 4 cut(s) 99, 567, 780, 861
Sse9I AATT 6 cut(s) 691, 703, 763, 843, 951, 1075
SsiI CCGC 5 cut(s) 166, 232, 286, 304, 744
SstI GAGCTC 1 cut(s) 740
StyD4I CCNGG 3 cut(s) 74, 334, 364
StyI CCWWGG 2 cut(s) 132, 553
TaaI ACNGT 4 cut(s) 311, 575, 754, 983
TaiI ACGT 4 cut(s) 259, 324, 537, 1126
TaqI TCGA 9 cut(s) 151, 210, 477, 656, 668, 994, 1058, 1163, 1243
TasI AATT 6 cut(s) 691, 703, 763, 843, 951, 1075
TatI WGTACW 1 cut(s) 1044
TauI GCSGC 2 cut(s) 288, 306
TfiI GAWTC 5 cut(s) 388, 526, 559, 809, 1055
Tru1I TTAA 7 cut(s) 66, 354, 872, 918, 1103, 1224, 1249
Tru9I TTAA 7 cut(s) 66, 354, 872, 918, 1103, 1224, 1249
TscAI CASTG 1 cut(s) 1076
TseFI GTSAC 4 cut(s) 7, 377, 644, 1030
TseI GCWGC 2 cut(s) 128, 241
Tsp45I GTSAC 4 cut(s) 7, 377, 644, 1030
TspDTI ATGAA 6 cut(s) 99, 111, 135, 473, 551, 801
TspGWI ACGGA 4 cut(s) 236, 365, 600, 1122
TspRI CASTG 1 cut(s) 1076
VpaK11BI GGWCC 2 cut(s) 78, 968
XceI RCATGY 1 cut(s) 104
XmnI GAANNNNTTC 1 cut(s) 464
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.