FvH4_2g19540

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008200
Physical Location & Seq
Forward (+)
16580146 .. 16584632
4487 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19540.t1

Sequence Viewer

Length: 1344 bp
ATGAGGTTAGCTAGCTCGAGGGCCACAATGCGGTTCTACATGACTCCCTTGCTGCTTCTTTTGGCCTTGCTCGTTTGCGTAGCGGCTTCCGTAGAGAATGACAAGCCTGTACACTCGAGGTTTGTGGAAGTAGAGGAGCCAAAGAGCTCCTTCAACTCGTCAATGGCAGACAGGTCCAACGACGACTGGAATGAGCACGCAGTAGATAATCCGGAGGAGATCGCGTCTCTGGTTGATACGAGCATTCGTAACAGTTCCGCGAGAAGAGAATTGGGATATTTCTCATGCGCAACAGGGAATCCCATTGATGACTGCTGGAGATGTGACCCGCAATGGCAGCGCCACCGCAAGAGGCTAGCCAACTGCGGTATTGGATTCGGCCGCAACGCCGTCGGTGGCCGTGATGGAAAGTACTACGTCGTAAGTGACCCCGGTCATGATGACCCGGTGAACCCCCGGCCGGGAACCCTCCGTCACGCTGTCATCCAAGACAGGCCTTTGTGGATTGTGTTCAAGCGTGACATGGTGATCACATTGAAGCAGGAGCTTATAATGAACAGCTTCAAGACCATTGACGCTAGAGGTGTCAATGTCCACATTGCTTATGGAGGTTGCATTACTATTCAGTTTGTTACTAATGTGATCATCCATGGTCTACACATCCACGACTGCAAGCCTACCGGAAATGCCATGGTCCGGAGCTCGCCGAGTCATTACGGGTGGAGGACCATGGCTGATGGTGATGGTATATCCATATTCGGATCTAGCCACATTTGGGTTGATCACAACTCGCTCTCGAATTGCGCCGATGGCCTCATTGATGCTATCATGGGTTCTACTGCCATTACCATTTCCAACAACTACTTCACTCACCATAATGAGGTTATGCTGTTGGGGCATAGTGACTCCTACACAAGGGACAAGCAGATGCAAGTGACCATTGCCTACAATCATTTTGGTGAAGGACTTATCCAAAGAATGCCAAGATGTAGACATGGGTATTTCCATGTGGTGAACAATGACTACACTCACTGGGAGATGTATGCCATTGGTGGAAGTGCTGATCCTACCATTAACAGTCAGGGCAACAGATATCTCGCCCCTAACAACCGTTTTGCTAAAGAGGTGACACATAGAGTTCAGACCACAGGCAGATGGAGGCACTGGAACTGGAGATCAGAGGGAGACCTACTCCTTAACGGAGCCTATTTCGTCCAATCCGGAGCTGGTGCTGCAGCCAGTTATGCAAGAGCTTCAAGCTTAGGGGCAAAGTCATCTTCCATGATTGGCACCATTACTGCTGGTGCTGGTGTTCTTAACTGCCGGTTTGGTCGTCAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

448

Amino Acids

49.81

Weight (kDa)

8.44

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 187 - 379 2.1e-18 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 551
Acc16I TGCGCA 1 cut(s) 289
AccB1I GGYRCC 1 cut(s) 1289
AccI GTMKAC 2 cut(s) 655, 991
AccII CGCG 2 cut(s) 224, 260
AccIII TCCGGA 3 cut(s) 211, 696, 1220
AciI CCGC 7 cut(s) 31, 83, 258, 329, 346, 366, 382
AclWI GGATC 2 cut(s) 769, 1058
AcoI YGGCCR 3 cut(s) 379, 397, 458
AfaI GTAC 2 cut(s) 111, 413
AfiI CCNNNNNNNGG 7 cut(s) 30, 460, 461, 696, 775, 880, 915
AgsI TTSAA 5 cut(s) 154, 514, 538, 565, 1257
AjuI GAANNNNNNNTTGG 2 cut(s) 848, 880
AluBI AGCT 9 cut(s) 11, 15, 147, 547, 561, 702, 1226, 1253, 1260
AluI AGCT 9 cut(s) 11, 15, 147, 547, 561, 702, 1226, 1253, 1260
Alw21I GWGCWC 3 cut(s) 149, 198, 704
Alw26I GTCTC 2 cut(s) 231, 1179
AlwI GGATC 2 cut(s) 769, 1058
Ama87I CYCGRG 2 cut(s) 16, 115
Aor13HI TCCGGA 3 cut(s) 211, 696, 1220
AoxI GGCC 7 cut(s) 21, 63, 379, 397, 458, 494, 811
ApeKI GCWGC 4 cut(s) 52, 337, 1232, 1235
Asp700I GAANNNNTTC 1 cut(s) 560
AspLEI GCGC 3 cut(s) 290, 342, 806
AspS9I GGNCC 4 cut(s) 21, 174, 694, 726
AsuC2I CCSGG 4 cut(s) 432, 446, 457, 462
AsuHPI GGTGA 7 cut(s) 460, 538, 752, 863, 971, 1024, 1138
AsuNHI GCTAGC 2 cut(s) 11, 355
AvaI CYCGRG 2 cut(s) 16, 115
AvaII GGWCC 3 cut(s) 174, 694, 726
BanI GGYRCC 1 cut(s) 1289
BanII GRGCYC 2 cut(s) 149, 704
Bbv12I GWGCWC 3 cut(s) 149, 198, 704
BbvI GCAGC 4 cut(s) 39, 349, 1219, 1247
BccI CCATC 5 cut(s) 398, 731, 737, 803, 1149
BceAI ACGGC 2 cut(s) 374, 384
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BclI TGATCA 3 cut(s) 528, 642, 781
BcnI CCSGG 4 cut(s) 432, 446, 457, 462
BcoDI GTCTC 2 cut(s) 231, 1179
BfaI CTAG 4 cut(s) 12, 356, 579, 765
BfmI CTRYAG 1 cut(s) 1233
BfoI RGCGCY 1 cut(s) 343
BisI GCNGC 6 cut(s) 53, 84, 338, 382, 1233, 1236
BlsI GCNGC 6 cut(s) 54, 85, 339, 383, 1234, 1237
BmcAI AGTACT 1 cut(s) 413
Bme1390I CCNGG 4 cut(s) 432, 446, 457, 462
Bme18I GGWCC 3 cut(s) 174, 694, 726
BmeT110I CYCGRG 2 cut(s) 16, 115
BmgT120I GGNCC 4 cut(s) 21, 174, 694, 726
BmiI GGNNCC 4 cut(s) 138, 466, 1204, 1291
BmrFI CCNGG 4 cut(s) 432, 446, 457, 462
BmrI ACTGGG 1 cut(s) 1042
BmsI GCATC 2 cut(s) 811, 918
BmtI GCTAGC 2 cut(s) 15, 359
BmuI ACTGGG 1 cut(s) 1042
BoxI GACNNNNGTC 1 cut(s) 432
BplI GAGNNNNNCTC 2 cut(s) 1176, 1208
BpmI CTGGAG 2 cut(s) 337, 1192
Bpu10I CCTNAGC 1 cut(s) 1261
BpuMI CCSGG 4 cut(s) 432, 446, 457, 462
BsaI GGTCTC 1 cut(s) 1179
BsaJI CCNNGG 5 cut(s) 430, 455, 649, 690, 729
BsaWI WCCGGW 4 cut(s) 211, 680, 696, 1220
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 7 cut(s) 30, 460, 461, 696, 775, 880, 915
Bse118I RCCGGY 1 cut(s) 1323
Bse1I ACTGG 5 cut(s) 191, 1037, 1169, 1175, 1239
Bse3DI GCAATG 3 cut(s) 338, 597, 939
BseAI TCCGGA 3 cut(s) 211, 696, 1220
BseDI CCNNGG 5 cut(s) 430, 455, 649, 690, 729
BseGI GGATG 3 cut(s) 483, 645, 660
BseLI CCNNNNNNNGG 7 cut(s) 30, 460, 461, 696, 775, 880, 915
BseMI GCAATG 3 cut(s) 338, 597, 939
BseNI ACTGG 5 cut(s) 191, 1037, 1169, 1175, 1239
BseRI GAGGAG 2 cut(s) 149, 230
BseX3I CGGCCG 2 cut(s) 379, 458
BseXI GCAGC 4 cut(s) 39, 349, 1219, 1247
Bsh1236I CGCG 2 cut(s) 224, 260
Bsh1285I CGRYCG 2 cut(s) 382, 461
BshFI GGCC 7 cut(s) 23, 65, 381, 399, 460, 496, 813
BshNI GGYRCC 1 cut(s) 1289
BsiEI CGRYCG 2 cut(s) 382, 461
BsiHKAI GWGCWC 3 cut(s) 149, 198, 704
BsiHKCI CYCGRG 2 cut(s) 16, 115
BsiSI CCGG 9 cut(s) 212, 432, 446, 457, 461, 681, 697, 1221, 1324
BslFI GGGAC 1 cut(s) 932
BslI CCNNNNNNNGG 7 cut(s) 30, 460, 461, 696, 775, 880, 915
BsmAI GTCTC 2 cut(s) 231, 1179
BsmBI CGTCTC 1 cut(s) 231
BsmFI GGGAC 1 cut(s) 932
BsmI GAATGC 2 cut(s) 243, 984
BsnI GGCC 7 cut(s) 23, 65, 381, 399, 460, 496, 813
Bso31I GGTCTC 1 cut(s) 1179
BsoBI CYCGRG 2 cut(s) 16, 115
Bsp1286I GDGCHC 3 cut(s) 149, 198, 704
Bsp13I TCCGGA 3 cut(s) 211, 696, 1220
Bsp1407I TGTACA 1 cut(s) 109
Bsp143I GATC 7 cut(s) 219, 528, 642, 761, 781, 1063, 1175
Bsp19I CCATGG 3 cut(s) 649, 690, 729
BspACI CCGC 7 cut(s) 31, 83, 258, 329, 346, 366, 382
BspANI GGCC 7 cut(s) 23, 65, 381, 399, 460, 496, 813
BspEI TCCGGA 3 cut(s) 211, 696, 1220
BspFNI CGCG 2 cut(s) 224, 260
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 4 cut(s) 138, 466, 1204, 1291
BspMAI CTGCAG 1 cut(s) 1237
BspOI GCTAGC 2 cut(s) 15, 359
BspPI GGATC 2 cut(s) 769, 1058
BspT107I GGYRCC 1 cut(s) 1289
BspTNI GGTCTC 1 cut(s) 1179
BsrDI GCAATG 3 cut(s) 338, 597, 939
BsrFI RCCGGY 1 cut(s) 1323
BsrGI TGTACA 1 cut(s) 109
BsrI ACTGG 5 cut(s) 191, 1037, 1169, 1175, 1239
BssAI RCCGGY 1 cut(s) 1323
BssECI CCNNGG 5 cut(s) 430, 455, 649, 690, 729
BssMI GATC 7 cut(s) 219, 528, 642, 761, 781, 1063, 1175
BssT1I CCWWGG 3 cut(s) 649, 690, 729
Bst4CI ACNGT 3 cut(s) 254, 1079, 1112
Bst6I CTCTTC 1 cut(s) 259
BstAUI TGTACA 1 cut(s) 109
BstC8I GCNNGC 5 cut(s) 13, 198, 357, 674, 704
BstDEI CTNAG 1 cut(s) 1261
BstDSI CCRYGG 3 cut(s) 649, 690, 729
BstENI CCTNNNNNAGG 1 cut(s) 913
BstF5I GGATG 3 cut(s) 483, 645, 660
BstFNI CGCG 2 cut(s) 224, 260
BstH2I RGCGCY 1 cut(s) 343
BstHHI GCGC 3 cut(s) 290, 342, 806
BstKTI GATC 7 cut(s) 222, 531, 645, 764, 784, 1066, 1178
BstMAI GTCTC 2 cut(s) 231, 1179
BstMBI GATC 7 cut(s) 219, 528, 642, 761, 781, 1063, 1175
BstMCI CGRYCG 2 cut(s) 382, 461
BstMWI GCNNNNNNNGC 5 cut(s) 337, 810, 895, 1232, 1244
BstPAI GACNNNNGTC 1 cut(s) 432
BstSCI CCNGG 4 cut(s) 430, 444, 455, 460
BstSFI CTRYAG 1 cut(s) 1233
BstUI CGCG 2 cut(s) 224, 260
BstV1I GCAGC 4 cut(s) 39, 349, 1219, 1247
BstX2I RGATCY 1 cut(s) 761
BstYI RGATCY 1 cut(s) 761
BstZI CGGCCG 2 cut(s) 379, 458
BsuRI GGCC 7 cut(s) 23, 65, 381, 399, 460, 496, 813
BtgI CCRYGG 3 cut(s) 649, 690, 729
BtsCI GGATG 3 cut(s) 483, 645, 660
BtsIMutI CAGTG 2 cut(s) 1030, 1162
Cac8I GCNNGC 5 cut(s) 13, 198, 357, 674, 704
CciI TCATGA 1 cut(s) 436
CfoI GCGC 3 cut(s) 290, 342, 806
Cfr10I RCCGGY 1 cut(s) 1323
Cfr13I GGNCC 4 cut(s) 21, 174, 694, 726
CseI GACGC 2 cut(s) 213, 584
Csp6I GTAC 2 cut(s) 110, 412
CviQI GTAC 2 cut(s) 110, 412
DdeI CTNAG 1 cut(s) 1261
DpnI GATC 7 cut(s) 221, 530, 644, 763, 783, 1065, 1177
DpnII GATC 7 cut(s) 219, 528, 642, 761, 781, 1063, 1175
EaeI YGGCCR 3 cut(s) 379, 397, 458
EagI CGGCCG 2 cut(s) 379, 458
Eam1104I CTCTTC 1 cut(s) 259
EarI CTCTTC 1 cut(s) 259
Ecl136II GAGCTC 2 cut(s) 147, 702
EclXI CGGCCG 2 cut(s) 379, 458
Eco130I CCWWGG 3 cut(s) 649, 690, 729
Eco147I AGGCCT 1 cut(s) 496
Eco24I GRGCYC 2 cut(s) 149, 704
Eco31I GGTCTC 1 cut(s) 1179
Eco32I GATATC 1 cut(s) 1094
Eco47I GGWCC 3 cut(s) 174, 694, 726
Eco52I CGGCCG 2 cut(s) 379, 458
Eco53kI GAGCTC 2 cut(s) 147, 702
Eco88I CYCGRG 2 cut(s) 16, 115
EcoICRI GAGCTC 2 cut(s) 147, 702
EcoNI CCTNNNNNAGG 1 cut(s) 913
EcoRV GATATC 1 cut(s) 1094
EcoT14I CCWWGG 3 cut(s) 649, 690, 729
EcoT38I GRGCYC 2 cut(s) 149, 704
ErhI CCWWGG 3 cut(s) 649, 690, 729
Esp3I CGTCTC 1 cut(s) 231
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 1 cut(s) 932
FauI CCCGC 1 cut(s) 336
FbaI TGATCA 3 cut(s) 528, 642, 781
FblI GTMKAC 2 cut(s) 655, 991
Fnu4HI GCNGC 6 cut(s) 53, 84, 338, 382, 1233, 1236
FokI GGATG 3 cut(s) 470, 632, 647
FriOI GRGCYC 2 cut(s) 149, 704
Fsp4HI GCNGC 6 cut(s) 53, 84, 338, 382, 1233, 1236
FspBI CTAG 4 cut(s) 12, 356, 579, 765
FspI TGCGCA 1 cut(s) 289
GlaI GCGC 3 cut(s) 289, 341, 805
GluI GCNGC 6 cut(s) 53, 84, 338, 382, 1233, 1236
GsuI CTGGAG 2 cut(s) 337, 1192
HaeII RGCGCY 1 cut(s) 343
HaeIII GGCC 7 cut(s) 23, 65, 381, 399, 460, 496, 813
HapII CCGG 9 cut(s) 212, 432, 446, 457, 461, 681, 697, 1221, 1324
HgaI GACGC 2 cut(s) 213, 584
HhaI GCGC 3 cut(s) 290, 342, 806
Hin6I GCGC 3 cut(s) 288, 340, 804
HinP1I GCGC 3 cut(s) 288, 340, 804
HindIII AAGCTT 1 cut(s) 1258
HinfI GANTC 5 cut(s) 43, 298, 375, 709, 905
HpaII CCGG 9 cut(s) 212, 432, 446, 457, 461, 681, 697, 1221, 1324
HphI GGTGA 7 cut(s) 460, 538, 752, 863, 971, 1024, 1138
Hpy166II GTNNAC 6 cut(s) 112, 451, 595, 656, 992, 1015
Hpy188I TCNGA 3 cut(s) 761, 1143, 1180
Hpy188III TCNNGA 6 cut(s) 212, 437, 565, 697, 796, 1221
Hpy8I GTNNAC 6 cut(s) 112, 451, 595, 656, 992, 1015
Hpy99I CGWCG 3 cut(s) 185, 395, 422
HpyAV CCTTC 2 cut(s) 160, 956
HpyCH4III ACNGT 3 cut(s) 254, 1079, 1112
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 5 cut(s) 615, 672, 931, 1235, 1247
HpyF10VI GCNNNNNNNGC 5 cut(s) 337, 810, 895, 1232, 1244
HpyF3I CTNAG 1 cut(s) 1261
HpySE526I ACGT 1 cut(s) 417
HspAI GCGC 3 cut(s) 288, 340, 804
Kpn2I TCCGGA 3 cut(s) 211, 696, 1220
Ksp22I TGATCA 3 cut(s) 528, 642, 781
Kzo9I GATC 7 cut(s) 219, 528, 642, 761, 781, 1063, 1175
LmnI GCTCC 6 cut(s) 136, 152, 544, 699, 1202, 1223
Lsp1109I GCAGC 4 cut(s) 39, 349, 1219, 1247
LweI GCATC 2 cut(s) 811, 918
MaeI CTAG 4 cut(s) 12, 356, 579, 765
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 9 cut(s) 248, 323, 425, 473, 518, 631, 902, 934, 1126
MalI GATC 7 cut(s) 221, 530, 644, 763, 783, 1065, 1177
MboI GATC 7 cut(s) 219, 528, 642, 761, 781, 1063, 1175
MboII GAAGA 2 cut(s) 276, 1269
MflI RGATCY 1 cut(s) 761
MhlI GDGCHC 3 cut(s) 149, 198, 704
MluCI AATT 2 cut(s) 269, 799
MlyI GAGTC 3 cut(s) 37, 718, 899
MmeI TCCRAC 2 cut(s) 201, 879
MroI TCCGGA 3 cut(s) 211, 696, 1220
MroXI GAANNNNTTC 1 cut(s) 560
MseI TTAA 4 cut(s) 1074, 1197, 1317, 1342
MslI CAYNNNNRTG 2 cut(s) 876, 957
MspI CCGG 9 cut(s) 212, 432, 446, 457, 461, 681, 697, 1221, 1324
MspR9I CCNGG 4 cut(s) 432, 446, 457, 462
Mva1269I GAATGC 2 cut(s) 243, 984
MvnI CGCG 2 cut(s) 224, 260
MwoI GCNNNNNNNGC 5 cut(s) 337, 810, 895, 1232, 1244
NciI CCSGG 4 cut(s) 432, 446, 457, 462
NcoI CCATGG 3 cut(s) 649, 690, 729
NdeII GATC 7 cut(s) 219, 528, 642, 761, 781, 1063, 1175
NheI GCTAGC 2 cut(s) 11, 355
NlaIV GGNNCC 4 cut(s) 138, 466, 1204, 1291
NmeAIII GCCGAG 1 cut(s) 732
NmuCI GTSAC 7 cut(s) 323, 425, 473, 518, 902, 934, 1126
NsbI TGCGCA 1 cut(s) 289
PaeR7I CTCGAG 2 cut(s) 16, 115
PagI TCATGA 1 cut(s) 436
PceI AGGCCT 1 cut(s) 496
PctI GAATGC 2 cut(s) 243, 984
PdmI GAANNNNTTC 1 cut(s) 560
PfeI GAWTC 2 cut(s) 298, 375
PkrI GCNGC 6 cut(s) 54, 85, 339, 383, 1234, 1237
PleI GAGTC 3 cut(s) 37, 717, 899
PpsI GAGTC 3 cut(s) 37, 717, 899
PshAI GACNNNNGTC 1 cut(s) 432
PsiI TTATAA 1 cut(s) 551
Psp124BI GAGCTC 2 cut(s) 149, 704
PspN4I GGNNCC 4 cut(s) 138, 466, 1204, 1291
PspPI GGNCC 4 cut(s) 21, 174, 694, 726
PspXI VCTCGAGB 2 cut(s) 16, 115
PsrI GAACNNNNNNTAC 2 cut(s) 1007, 1039
PstI CTGCAG 1 cut(s) 1237
PsuI RGATCY 1 cut(s) 761
RsaI GTAC 2 cut(s) 111, 413
RsaNI GTAC 2 cut(s) 110, 412
RseI CAYNNNNRTG 2 cut(s) 876, 957
SacI GAGCTC 2 cut(s) 149, 704
SaqAI TTAA 4 cut(s) 1074, 1197, 1317, 1342
SatI GCNGC 6 cut(s) 53, 84, 338, 382, 1233, 1236
Sau3AI GATC 7 cut(s) 219, 528, 642, 761, 781, 1063, 1175
Sau96I GGNCC 4 cut(s) 21, 174, 694, 726
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 3 cut(s) 37, 718, 899
ScrFI CCNGG 4 cut(s) 432, 446, 457, 462
SduI GDGCHC 3 cut(s) 149, 198, 704
SfaNI GCATC 2 cut(s) 811, 918
SfcI CTRYAG 1 cut(s) 1233
Sfr274I CTCGAG 2 cut(s) 16, 115
SinI GGWCC 3 cut(s) 174, 694, 726
SlaI CTCGAG 2 cut(s) 16, 115
SmiMI CAYNNNNRTG 2 cut(s) 876, 957
SmlI CTYRAG 2 cut(s) 16, 115
SmoI CTYRAG 2 cut(s) 16, 115
Sse9I AATT 2 cut(s) 269, 799
SseBI AGGCCT 1 cut(s) 496
SsiI CCGC 7 cut(s) 31, 83, 258, 329, 346, 366, 382
SspMI CTAG 4 cut(s) 12, 356, 579, 765
SstI GAGCTC 2 cut(s) 149, 704
StuI AGGCCT 1 cut(s) 496
StyD4I CCNGG 4 cut(s) 430, 444, 455, 460
StyI CCWWGG 3 cut(s) 649, 690, 729
TaaI ACNGT 3 cut(s) 254, 1079, 1112
TaiI ACGT 1 cut(s) 420
TaqI TCGA 3 cut(s) 17, 116, 797
TasI AATT 2 cut(s) 269, 799
TatI WGTACW 2 cut(s) 109, 411
TauI GCSGC 2 cut(s) 86, 384
TfiI GAWTC 2 cut(s) 298, 375
Tru1I TTAA 4 cut(s) 1074, 1197, 1317, 1342
Tru9I TTAA 4 cut(s) 1074, 1197, 1317, 1342
TscAI CASTG 2 cut(s) 1037, 1169
TseFI GTSAC 7 cut(s) 323, 425, 473, 518, 902, 934, 1126
TseI GCWGC 4 cut(s) 52, 337, 1232, 1235
Tsp45I GTSAC 7 cut(s) 323, 425, 473, 518, 902, 934, 1126
TspDTI ATGAA 1 cut(s) 569
TspGWI ACGGA 3 cut(s) 79, 461, 1215
TspRI CASTG 2 cut(s) 1037, 1169
VpaK11BI GGWCC 3 cut(s) 174, 694, 726
XagI CCTNNNNNAGG 1 cut(s) 913
XcmI CCANNNNNNNNNTGG 2 cut(s) 602, 1223
XhoI CTCGAG 2 cut(s) 16, 115
XmiI GTMKAC 2 cut(s) 655, 991
XmnI GAANNNNTTC 1 cut(s) 560
XspI CTAG 4 cut(s) 12, 356, 579, 765
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.