Rh6DG279400

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008200
Physical Location & Seq
Forward (+)
47359173 .. 47363634
4462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG279400.1

Sequence Viewer

Length: 1347 bp
ATGAGATTAGCTAGCTCGAAAGCCACAATGCGGTTCTATATGACTCCACTGCTGCTTCTTTTGGCCTTGCTCGTAAGCGTAGCGGCTTCAATAGAGAATGACAAGCCTTTACAGTCAAGGTCCGTGGGACTAGAGGAGCCAAAAAGCTCCATCAACTCGTCAATGTCGACAGAAAGGTCCAACGATGACTGGAATGAGCACGCAGTAGATAATCCGGAGGAGATCGCGTCCTTGGTTGATACGACCATTCGTAACAGTACTGCGAGAAGAAACTTGGGATTTTTCTCATGCGCAACAGGGAATCCCATTGATGACTGCTGGAGATGTGACCCACAATGGCAGCGCCACCGCAAGAGGCTAGCCAATTGCGGCATTGGGTTCGGGCGCAATGCCGTAGGCGGCCGTGACGGAAGGTATTACGTTGTAAATGACCCCGGTCATGATGATCCGGTGAACCCCAGGCCCGGTACCCTCCGTCACGCTGTCATCCAGGACAGGCCTTTGTGGATTGTGTTCAAGCGTGATATGGTGATCACATTGAAGCAGGAGCTTATAATGAACAGCTTCAAGACCATTGACGCTCGTGGAGTCAATGTCCACATTGCTTATGGAGGTTGCATTACTATTCAATACGTTACTAATGTGATCATCCATGGTCTACATATCCATGACTGCAAGCCTACCGGCAATGCCATGGTCCGGAGCTCACCGAGTCATTATGGGTGGAGGACCATGGCTGATGGGGATGGTATATCCATTTTCGGAGCCAGCCACATTTGGGTCGACCACAACTCGCTCTCGAATTGCGCCGATGGCCTCATTGATGCTATCATGGGTTCTACTGCCATTACCATTTCTAACAACTACTTCACTCACCATAATGAGGTTATGTTGTTGGGGCACAGTGATTCCTACACAAGGGACAAGCAAATGCAAGTGACCATTGCCTACAATCATTTTGGTGAGGGACTCATCCAAAGAATGCCAAGATGTAGACATGGGTATTTCCATGTGGTGAACAATGACTACACTCACTGGGAAATGTATGCCATTGGTGGAAGTGCTGATCCCACCATTAACAGCCAGGGCAACAGATATCTCGCCCCTACCAACCGTTTTGCCAAAGAGGTGACACATAGAGTTGAGACCACAGGCAGATGGAGGCACTGGAACTGGAGATCAGAAGGAGACCTACTCCTTAATGGAGCGTTTTTCGTCCAATCCGGAGCTGGTGCTGCAGCCAGTTATGCCAGAGCTTCAAGCTTAGGGGCAAAGTCATCTTCCATGATTGGCAGCATTACTGCTGGTGCTGGTGTTCTTAATTGCCGGTCTGGTCGTCAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

448

Amino Acids

49.71

Weight (kDa)

8.64

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 187 - 372 6.5e-18 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
AasI GACNNNNNNGTC 1 cut(s) 175
Acc16I TGCGCA 1 cut(s) 292
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 467
AccI GTMKAC 4 cut(s) 167, 658, 783, 994
AccII CGCG 1 cut(s) 227
AccIII TCCGGA 3 cut(s) 214, 699, 1223
AciI CCGC 5 cut(s) 31, 83, 349, 369, 399
AclWI GGATC 2 cut(s) 440, 1061
AcoI YGGCCR 1 cut(s) 400
AfaI GTAC 2 cut(s) 259, 469
AfiI CCNNNNNNNGG 6 cut(s) 30, 464, 699, 778, 883, 918
AgsI TTSAA 6 cut(s) 90, 517, 541, 568, 629, 1260
AjnI CCWGG 3 cut(s) 458, 489, 1083
AluBI AGCT 9 cut(s) 11, 15, 147, 550, 564, 705, 1229, 1256, 1263
AluI AGCT 9 cut(s) 11, 15, 147, 550, 564, 705, 1229, 1256, 1263
Alw21I GWGCWC 2 cut(s) 201, 707
Alw26I GTCTC 2 cut(s) 1139, 1182
AlwI GGATC 2 cut(s) 440, 1061
Aor13HI TCCGGA 3 cut(s) 214, 699, 1223
AoxI GGCC 5 cut(s) 63, 400, 461, 497, 814
ApeKI GCWGC 5 cut(s) 52, 340, 1235, 1238, 1293
Asp700I GAANNNNTTC 1 cut(s) 563
Asp718I GGTACC 1 cut(s) 467
AspLEI GCGC 4 cut(s) 293, 345, 387, 809
AspS9I GGNCC 5 cut(s) 120, 177, 462, 697, 729
AsuC2I CCSGG 2 cut(s) 435, 465
AsuHPI GGTGA 7 cut(s) 463, 541, 699, 866, 974, 1027, 1141
AsuNHI GCTAGC 2 cut(s) 11, 358
AvaII GGWCC 4 cut(s) 120, 177, 697, 729
BaeGI GKGCMC 1 cut(s) 903
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 707
BarI GAAGNNNNNNTAC 2 cut(s) 1176, 1208
BauI CACGAG 1 cut(s) 582
Bbv12I GWGCWC 2 cut(s) 201, 707
BbvI GCAGC 5 cut(s) 39, 352, 1222, 1250, 1305
BccI CCATC 5 cut(s) 158, 734, 740, 806, 1152
BceAI ACGGC 2 cut(s) 377, 387
BcgI CGANNNNNNTGC 2 cut(s) 361, 395
BciT130I CCWGG 3 cut(s) 460, 491, 1085
BclI TGATCA 2 cut(s) 531, 645
BcnI CCSGG 2 cut(s) 435, 465
BcoDI GTCTC 2 cut(s) 1139, 1182
BfaI CTAG 3 cut(s) 12, 131, 359
BfmI CTRYAG 1 cut(s) 1236
BfoI RGCGCY 1 cut(s) 346
BisI GCNGC 8 cut(s) 53, 84, 341, 370, 400, 1236, 1239, 1294
BlsI GCNGC 8 cut(s) 54, 85, 342, 371, 401, 1237, 1240, 1295
BmcAI AGTACT 1 cut(s) 259
Bme1390I CCNGG 5 cut(s) 435, 460, 465, 491, 1085
Bme18I GGWCC 4 cut(s) 120, 177, 697, 729
BmgT120I GGNCC 5 cut(s) 120, 177, 462, 697, 729
BmiI GGNNCC 3 cut(s) 138, 469, 766
BmrFI CCNGG 5 cut(s) 435, 460, 465, 491, 1085
BmrI ACTGGG 1 cut(s) 1045
BmsI GCATC 1 cut(s) 814
BmtI GCTAGC 2 cut(s) 15, 362
BmuI ACTGGG 1 cut(s) 1045
BoxI GACNNNNGTC 1 cut(s) 435
BplI GAGNNNNNCTC 2 cut(s) 1179, 1211
BpmI CTGGAG 2 cut(s) 340, 1195
Bpu10I CCTNAGC 1 cut(s) 1264
BpuMI CCSGG 2 cut(s) 435, 465
BsaI GGTCTC 2 cut(s) 1139, 1182
BsaJI CCNNGG 8 cut(s) 123, 231, 433, 458, 652, 693, 732, 1084
BsaWI WCCGGW 4 cut(s) 214, 448, 699, 1223
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 579, 609
Bsc4I CCNNNNNNNGG 6 cut(s) 30, 464, 699, 778, 883, 918
Bse118I RCCGGY 2 cut(s) 683, 1326
Bse1I ACTGG 5 cut(s) 194, 1040, 1172, 1178, 1242
Bse3DI GCAATG 4 cut(s) 394, 600, 694, 942
BseAI TCCGGA 3 cut(s) 214, 699, 1223
BseBI CCWGG 3 cut(s) 460, 491, 1085
BseDI CCNNGG 8 cut(s) 123, 231, 433, 458, 652, 693, 732, 1084
BseGI GGATG 4 cut(s) 486, 648, 751, 972
BseLI CCNNNNNNNGG 6 cut(s) 30, 464, 699, 778, 883, 918
BseMI GCAATG 4 cut(s) 394, 600, 694, 942
BseNI ACTGG 5 cut(s) 194, 1040, 1172, 1178, 1242
BseRI GAGGAG 2 cut(s) 149, 233
BseSI GKGCMC 1 cut(s) 903
BseX3I CGGCCG 1 cut(s) 400
BseXI GCAGC 5 cut(s) 39, 352, 1222, 1250, 1305
Bsh1236I CGCG 1 cut(s) 227
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 5 cut(s) 65, 402, 463, 499, 816
BshNI GGYRCC 1 cut(s) 467
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 2 cut(s) 201, 707
BsiSI CCGG 8 cut(s) 215, 435, 449, 465, 684, 700, 1224, 1327
BslFI GGGAC 3 cut(s) 141, 935, 981
BslI CCNNNNNNNGG 6 cut(s) 30, 464, 699, 778, 883, 918
BsmAI GTCTC 2 cut(s) 1139, 1182
BsmFI GGGAC 3 cut(s) 141, 935, 981
BsmI GAATGC 1 cut(s) 987
BsnI GGCC 5 cut(s) 65, 402, 463, 499, 816
Bso31I GGTCTC 2 cut(s) 1139, 1182
Bsp1286I GDGCHC 3 cut(s) 201, 707, 903
Bsp13I TCCGGA 3 cut(s) 214, 699, 1223
Bsp143I GATC 6 cut(s) 222, 445, 531, 645, 1066, 1178
Bsp19I CCATGG 3 cut(s) 652, 693, 732
BspACI CCGC 5 cut(s) 31, 83, 349, 369, 399
BspANI GGCC 5 cut(s) 65, 402, 463, 499, 816
BspEI TCCGGA 3 cut(s) 214, 699, 1223
BspFNI CGCG 1 cut(s) 227
BspHI TCATGA 1 cut(s) 439
BspLI GGNNCC 3 cut(s) 138, 469, 766
BspMAI CTGCAG 1 cut(s) 1240
BspOI GCTAGC 2 cut(s) 15, 362
BspPI GGATC 2 cut(s) 440, 1061
BspT107I GGYRCC 1 cut(s) 467
BspTNI GGTCTC 2 cut(s) 1139, 1182
BsrDI GCAATG 4 cut(s) 394, 600, 694, 942
BsrFI RCCGGY 2 cut(s) 683, 1326
BsrI ACTGG 5 cut(s) 194, 1040, 1172, 1178, 1242
BssAI RCCGGY 2 cut(s) 683, 1326
BssECI CCNNGG 8 cut(s) 123, 231, 433, 458, 652, 693, 732, 1084
BssMI GATC 6 cut(s) 222, 445, 531, 645, 1066, 1178
BssSI CACGAG 1 cut(s) 582
BssT1I CCWWGG 4 cut(s) 231, 652, 693, 732
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 3 cut(s) 460, 491, 1085
Bst4CI ACNGT 4 cut(s) 114, 257, 905, 1115
BstC8I GCNNGC 5 cut(s) 13, 201, 360, 677, 769
BstDEI CTNAG 1 cut(s) 1264
BstDSI CCRYGG 4 cut(s) 123, 652, 693, 732
BstENI CCTNNNNNAGG 1 cut(s) 916
BstF5I GGATG 4 cut(s) 486, 648, 751, 972
BstFNI CGCG 1 cut(s) 227
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 4 cut(s) 293, 345, 387, 809
BstKTI GATC 6 cut(s) 225, 448, 534, 648, 1069, 1181
BstMAI GTCTC 2 cut(s) 1139, 1182
BstMBI GATC 6 cut(s) 222, 445, 531, 645, 1066, 1178
BstMCI CGRYCG 1 cut(s) 403
BstMWI GCNNNNNNNGC 3 cut(s) 813, 1235, 1247
BstNI CCWGG 3 cut(s) 460, 491, 1085
BstPAI GACNNNNGTC 1 cut(s) 435
BstSCI CCNGG 5 cut(s) 433, 458, 463, 489, 1083
BstSFI CTRYAG 1 cut(s) 1236
BstSLI GKGCMC 1 cut(s) 903
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 5 cut(s) 39, 352, 1222, 1250, 1305
BstZI CGGCCG 1 cut(s) 400
BsuRI GGCC 5 cut(s) 65, 402, 463, 499, 816
BtgI CCRYGG 4 cut(s) 123, 652, 693, 732
BtsCI GGATG 4 cut(s) 486, 648, 751, 972
BtsI GCAGTG 1 cut(s) 47
BtsIMutI CAGTG 4 cut(s) 47, 910, 1033, 1165
Cac8I GCNNGC 5 cut(s) 13, 201, 360, 677, 769
CciI TCATGA 1 cut(s) 439
CfoI GCGC 4 cut(s) 293, 345, 387, 809
Cfr10I RCCGGY 2 cut(s) 683, 1326
Cfr13I GGNCC 5 cut(s) 120, 177, 462, 697, 729
CseI GACGC 2 cut(s) 216, 587
Csp6I GTAC 2 cut(s) 258, 468
CspCI CAANNNNNGTGG 2 cut(s) 105, 140
CviQI GTAC 2 cut(s) 258, 468
DdeI CTNAG 1 cut(s) 1264
DpnI GATC 6 cut(s) 224, 447, 533, 647, 1068, 1180
DpnII GATC 6 cut(s) 222, 445, 531, 645, 1066, 1178
DrdI GACNNNNNNGTC 1 cut(s) 175
DseDI GACNNNNNNGTC 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 400
EagI CGGCCG 1 cut(s) 400
Ecl136II GAGCTC 1 cut(s) 705
EclXI CGGCCG 1 cut(s) 400
Eco130I CCWWGG 4 cut(s) 231, 652, 693, 732
Eco147I AGGCCT 1 cut(s) 499
Eco24I GRGCYC 1 cut(s) 707
Eco31I GGTCTC 2 cut(s) 1139, 1182
Eco32I GATATC 1 cut(s) 1097
Eco47I GGWCC 4 cut(s) 120, 177, 697, 729
Eco52I CGGCCG 1 cut(s) 400
Eco53kI GAGCTC 1 cut(s) 705
EcoICRI GAGCTC 1 cut(s) 705
EcoNI CCTNNNNNAGG 1 cut(s) 916
EcoRII CCWGG 3 cut(s) 458, 489, 1083
EcoRV GATATC 1 cut(s) 1097
EcoT14I CCWWGG 4 cut(s) 231, 652, 693, 732
EcoT38I GRGCYC 1 cut(s) 707
ErhI CCWWGG 4 cut(s) 231, 652, 693, 732
FaqI GGGAC 3 cut(s) 141, 935, 981
FbaI TGATCA 2 cut(s) 531, 645
FblI GTMKAC 4 cut(s) 167, 658, 783, 994
Fnu4HI GCNGC 8 cut(s) 53, 84, 341, 370, 400, 1236, 1239, 1294
FokI GGATG 4 cut(s) 473, 635, 758, 959
FriOI GRGCYC 1 cut(s) 707
Fsp4HI GCNGC 8 cut(s) 53, 84, 341, 370, 400, 1236, 1239, 1294
FspBI CTAG 3 cut(s) 12, 131, 359
FspI TGCGCA 1 cut(s) 292
GlaI GCGC 4 cut(s) 292, 344, 386, 808
GluI GCNGC 8 cut(s) 53, 84, 341, 370, 400, 1236, 1239, 1294
GsuI CTGGAG 2 cut(s) 340, 1195
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 5 cut(s) 65, 402, 463, 499, 816
HapII CCGG 8 cut(s) 215, 435, 449, 465, 684, 700, 1224, 1327
HgaI GACGC 2 cut(s) 216, 587
HhaI GCGC 4 cut(s) 293, 345, 387, 809
Hin6I GCGC 4 cut(s) 291, 343, 385, 807
HinP1I GCGC 4 cut(s) 291, 343, 385, 807
HincII GTYRAC 2 cut(s) 168, 784
HindII GTYRAC 2 cut(s) 168, 784
HindIII AAGCTT 1 cut(s) 1261
HinfI GANTC 6 cut(s) 43, 301, 588, 712, 908, 969
HpaII CCGG 8 cut(s) 215, 435, 449, 465, 684, 700, 1224, 1327
HphI GGTGA 7 cut(s) 463, 541, 699, 866, 974, 1027, 1141
Hpy166II GTNNAC 7 cut(s) 168, 454, 598, 659, 784, 995, 1018
Hpy188I TCNGA 2 cut(s) 764, 1183
Hpy188III TCNNGA 6 cut(s) 215, 440, 568, 700, 799, 1224
Hpy8I GTNNAC 7 cut(s) 168, 454, 598, 659, 784, 995, 1018
HpyAV CCTTC 2 cut(s) 405, 1178
HpyCH4III ACNGT 4 cut(s) 114, 257, 905, 1115
HpyCH4IV ACGT 2 cut(s) 420, 633
HpyCH4V TGCA 4 cut(s) 618, 675, 934, 1238
HpyF10VI GCNNNNNNNGC 3 cut(s) 813, 1235, 1247
HpyF3I CTNAG 1 cut(s) 1264
HpySE526I ACGT 2 cut(s) 420, 633
HspAI GCGC 4 cut(s) 291, 343, 385, 807
Kpn2I TCCGGA 3 cut(s) 214, 699, 1223
KpnI GGTACC 1 cut(s) 471
Ksp22I TGATCA 2 cut(s) 531, 645
Kzo9I GATC 6 cut(s) 222, 445, 531, 645, 1066, 1178
LmnI GCTCC 7 cut(s) 136, 152, 547, 702, 764, 1205, 1226
Lsp1109I GCAGC 5 cut(s) 39, 352, 1222, 1250, 1305
LweI GCATC 1 cut(s) 814
MaeI CTAG 3 cut(s) 12, 131, 359
MaeII ACGT 2 cut(s) 420, 633
MaeIII GTNAC 7 cut(s) 251, 326, 404, 476, 634, 937, 1129
MalI GATC 6 cut(s) 224, 447, 533, 647, 1068, 1180
MboI GATC 6 cut(s) 222, 445, 531, 645, 1066, 1178
MboII GAAGA 2 cut(s) 279, 1272
MfeI CAATTG 1 cut(s) 364
MhlI GDGCHC 3 cut(s) 201, 707, 903
MluCI AATT 3 cut(s) 364, 802, 1321
MlyI GAGTC 4 cut(s) 37, 597, 721, 963
MmeI TCCRAC 1 cut(s) 204
MroI TCCGGA 3 cut(s) 214, 699, 1223
MroXI GAANNNNTTC 1 cut(s) 563
MseI TTAA 4 cut(s) 1077, 1200, 1320, 1345
MslI CAYNNNNRTG 3 cut(s) 666, 879, 960
MspI CCGG 8 cut(s) 215, 435, 449, 465, 684, 700, 1224, 1327
MspR9I CCNGG 5 cut(s) 435, 460, 465, 491, 1085
MunI CAATTG 1 cut(s) 364
Mva1269I GAATGC 1 cut(s) 987
MvaI CCWGG 3 cut(s) 460, 491, 1085
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 3 cut(s) 813, 1235, 1247
NciI CCSGG 2 cut(s) 435, 465
NcoI CCATGG 3 cut(s) 652, 693, 732
NdeII GATC 6 cut(s) 222, 445, 531, 645, 1066, 1178
NheI GCTAGC 2 cut(s) 11, 358
NlaIV GGNNCC 3 cut(s) 138, 469, 766
NmuCI GTSAC 5 cut(s) 326, 404, 476, 937, 1129
NsbI TGCGCA 1 cut(s) 292
PagI TCATGA 1 cut(s) 439
PceI AGGCCT 1 cut(s) 499
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PctI GAATGC 1 cut(s) 987
PdmI GAANNNNTTC 1 cut(s) 563
PfeI GAWTC 2 cut(s) 301, 908
PfoI TCCNGGA 1 cut(s) 489
PkrI GCNGC 8 cut(s) 54, 85, 342, 371, 401, 1237, 1240, 1295
PleI GAGTC 4 cut(s) 37, 596, 720, 963
PpsI GAGTC 4 cut(s) 37, 596, 720, 963
PshAI GACNNNNGTC 1 cut(s) 435
PsiI TTATAA 1 cut(s) 554
Psp124BI GAGCTC 1 cut(s) 707
Psp6I CCWGG 3 cut(s) 458, 489, 1083
PspGI CCWGG 3 cut(s) 458, 489, 1083
PspN4I GGNNCC 3 cut(s) 138, 469, 766
PspPI GGNCC 5 cut(s) 120, 177, 462, 697, 729
PsrI GAACNNNNNNTAC 2 cut(s) 1010, 1042
PstI CTGCAG 1 cut(s) 1240
RsaI GTAC 2 cut(s) 259, 469
RsaNI GTAC 2 cut(s) 258, 468
RseI CAYNNNNRTG 3 cut(s) 666, 879, 960
SacI GAGCTC 1 cut(s) 707
SalI GTCGAC 2 cut(s) 166, 782
SaqAI TTAA 4 cut(s) 1077, 1200, 1320, 1345
SatI GCNGC 8 cut(s) 53, 84, 341, 370, 400, 1236, 1239, 1294
Sau3AI GATC 6 cut(s) 222, 445, 531, 645, 1066, 1178
Sau96I GGNCC 5 cut(s) 120, 177, 462, 697, 729
ScaI AGTACT 1 cut(s) 259
SchI GAGTC 4 cut(s) 37, 597, 721, 963
ScrFI CCNGG 5 cut(s) 435, 460, 465, 491, 1085
SduI GDGCHC 3 cut(s) 201, 707, 903
SfaNI GCATC 1 cut(s) 814
SfcI CTRYAG 1 cut(s) 1236
SinI GGWCC 4 cut(s) 120, 177, 697, 729
SmiMI CAYNNNNRTG 3 cut(s) 666, 879, 960
Sse9I AATT 3 cut(s) 364, 802, 1321
SseBI AGGCCT 1 cut(s) 499
SsiI CCGC 5 cut(s) 31, 83, 349, 369, 399
SspMI CTAG 3 cut(s) 12, 131, 359
SstI GAGCTC 1 cut(s) 707
StuI AGGCCT 1 cut(s) 499
StyD4I CCNGG 5 cut(s) 433, 458, 463, 489, 1083
StyI CCWWGG 4 cut(s) 231, 652, 693, 732
TaaI ACNGT 4 cut(s) 114, 257, 905, 1115
TaiI ACGT 2 cut(s) 423, 636
TaqI TCGA 4 cut(s) 17, 167, 783, 800
TasI AATT 3 cut(s) 364, 802, 1321
TatI WGTACW 1 cut(s) 257
TauI GCSGC 3 cut(s) 86, 372, 402
TfiI GAWTC 2 cut(s) 301, 908
Tru1I TTAA 4 cut(s) 1077, 1200, 1320, 1345
Tru9I TTAA 4 cut(s) 1077, 1200, 1320, 1345
TscAI CASTG 4 cut(s) 54, 910, 1040, 1172
TseFI GTSAC 5 cut(s) 326, 404, 476, 937, 1129
TseI GCWGC 5 cut(s) 52, 340, 1235, 1238, 1293
Tsp45I GTSAC 5 cut(s) 326, 404, 476, 937, 1129
TspDTI ATGAA 1 cut(s) 572
TspGWI ACGGA 3 cut(s) 112, 423, 464
TspRI CASTG 4 cut(s) 54, 910, 1040, 1172
VpaK11BI GGWCC 4 cut(s) 120, 177, 697, 729
XagI CCTNNNNNAGG 1 cut(s) 916
XcmI CCANNNNNNNNNTGG 2 cut(s) 605, 1226
XmiI GTMKAC 4 cut(s) 167, 658, 783, 994
XmnI GAANNNNTTC 1 cut(s) 563
XspI CTAG 3 cut(s) 12, 131, 359
ZrmI AGTACT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.