MD05G1179500.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008200
Physical Location & Seq
Forward (+)
30728599 .. 30733872
5274 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1179500.v1.1.491

Sequence Viewer

Length: 1356 bp
ATGAGAGCTTACTCTAAAGCAACAATGGCGGTCTCTTTGAACTTGTTTTCGTTACTTTCGACAATGGCGGTTCTTCTAGCTCTGCTTGCAAGCACAAATGCCTCTGCAGAGAGTGGCAAGAATGTTAATTCAAGGTTTGTGGGACCAAAGGAGTCAATCAGCTCCAAGAACTCATCAATAGCAGAAAGGTTTGAAGAAGCTTTGAATGAGCACGCAGTAGATAATCCAGATGAAATTGCCTCTATGGTTGACATGAGCATTCGTAATAGCACAGAGAGAAGAAATTTGGGATTCTTTTCGTGTGCGACGGGGAATCCAATTGATGATTGTTGGAGGTGTGACCCACATTGGCAGCTCCACCGCAAGCGCTTAGCCAACTGCGGCATAGGGTTCGGGCGCAACGCCGTGGGCGGACGCGACGGGAAATACTACGTGGTGAACAACCCCGGCAACGACGATCCCGTCAACCCTAGAGCCGGAACACTCCGCCACGCTGTCATTCAGGATAGGCCGTTGTGGATTGTGTTCAAGCGTGACATGGTGATCACACTAAAGCAAGAGCTAATTATGAACAGCTTCAAGACAATTGATGCACGTGGAGTCAATGTGCACATAGCATATGGGGCATGCATTACGATTCAATATGTTACTAACGTCATCATCCATGGGCTGCATATCCATGACTGCAAACCCACCGGAAATGCCATGGTCCGGAGCTCGCCGAACCATTACGGGTGGAGAACGATGGCGGACGGGGACGCGATTTCCATTTTTGGATCCAGCCACATTTGGATCGACCATAATTCTTTTTCCAAATGTGCTGATGGGCTTGTTGATGCTATCATGGGCTCTACTGCACTTACCATTTCCAACAACTACTTCACCCACCATAATGAGGTTATGCTACTGGGCCACAGTGACTCCTATGTAAGGGACAAGCTTATGCAAGTGACAATTGCCTACAATCATTTTGGTGAGGGACTTATTCAGAGGATGCCAAGGTGTAGGCACGGGTATTTCCATGTGGTGAACAATGACTACACACATTGGGAGATGTATGCCATTGGTGGAAGTGCTGAACCCACAATTAACAGCCAGGGCAACAGATATCTGGCCCCTAACAATGGTTTTGCTAAGGAGGTGACACATAGAGTTAACCCAAATGACTGGAAGCACTGGAACTGGAGATCAGAAGGAGACCTGCTCCTAAATGGAGCCTATTTCATTGCATCAGGAACTGGTTCCGGAGCAAGCTATGCCAGGGCCTCTAGCTTGGGGGCCAAGTCATCTTCCATGGTTGGCTCCATCACAGCCAGCGCCGGCGTCCTCAACTGCCGGAGGGGTTTCCAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

452

Amino Acids

49.78

Weight (kDa)

8.61

Isoelectric Point (pI)

30.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 193 - 389 1.7e-17 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
Acc36I ACCTGC 1 cut(s) 1209
AccII CGCG 2 cut(s) 417, 761
AccIII TCCGGA 2 cut(s) 711, 1244
AciI CCGC 7 cut(s) 29, 68, 361, 381, 411, 487, 749
AclWI GGATC 4 cut(s) 452, 771, 784, 800
AcsI RAATTY 1 cut(s) 283
AcvI CACGTG 1 cut(s) 596
AcyI GRCGYC 1 cut(s) 1323
AfeI AGCGCT 1 cut(s) 368
AfiI CCNNNNNNNGG 5 cut(s) 476, 711, 895, 930, 1124
AgsI TTSAA 7 cut(s) 40, 132, 194, 205, 529, 580, 641
AjnI CCWGG 2 cut(s) 1095, 1259
AjuI GAANNNNNNNTTGG 2 cut(s) 863, 895
Alw21I GWGCWC 3 cut(s) 213, 612, 719
Alw26I GTCTC 2 cut(s) 37, 1191
Alw44I GTGCAC 1 cut(s) 608
AlwI GGATC 4 cut(s) 452, 771, 784, 800
AlwNI CAGNNNCTG 1 cut(s) 1238
Aor13HI TCCGGA 2 cut(s) 711, 1244
Aor51HI AGCGCT 1 cut(s) 368
AoxI GGCC 5 cut(s) 509, 910, 1113, 1263, 1278
ApaLI GTGCAC 1 cut(s) 608
ApeKI GCWGC 2 cut(s) 352, 670
ApoI RAATTY 1 cut(s) 283
Asp700I GAANNNNTTC 2 cut(s) 575, 1240
AspLEI GCGC 3 cut(s) 369, 399, 1319
AspS9I GGNCC 6 cut(s) 143, 709, 910, 1114, 1263, 1278
AsuC2I CCSGG 1 cut(s) 447
AsuHPI GGTGA 6 cut(s) 448, 553, 874, 986, 1039, 1153
AvaII GGWCC 2 cut(s) 143, 709
BaeGI GKGCMC 1 cut(s) 612
BamHI GGATCC 1 cut(s) 776
BanII GRGCYC 2 cut(s) 719, 851
BbrPI CACGTG 1 cut(s) 596
Bbv12I GWGCWC 3 cut(s) 213, 612, 719
BbvI GCAGC 2 cut(s) 364, 657
BccI CCATC 3 cut(s) 739, 818, 1313
BceAI ACGGC 2 cut(s) 389, 496
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BciT130I CCWGG 2 cut(s) 1097, 1261
BclI TGATCA 1 cut(s) 543
BcnI CCSGG 1 cut(s) 447
BcoDI GTCTC 2 cut(s) 37, 1191
BfaI CTAG 3 cut(s) 77, 471, 1269
BfmI CTRYAG 1 cut(s) 105
BfoI RGCGCY 2 cut(s) 370, 1320
BfuAI ACCTGC 1 cut(s) 1209
BisI GCNGC 3 cut(s) 353, 382, 671
BlpI GCTNAGC 1 cut(s) 370
BlsI GCNGC 3 cut(s) 354, 383, 672
Bme1390I CCNGG 3 cut(s) 447, 1097, 1261
Bme18I GGWCC 2 cut(s) 143, 709
BmgT120I GGNCC 6 cut(s) 143, 709, 910, 1114, 1263, 1278
BmiI GGNNCC 7 cut(s) 144, 778, 1116, 1216, 1243, 1279, 1303
BmrFI CCNGG 3 cut(s) 447, 1097, 1261
BmrI ACTGGG 1 cut(s) 917
BmsI GCATC 4 cut(s) 580, 826, 984, 1238
BmuI ACTGGG 1 cut(s) 917
BplI GAGNNNNNCTC 2 cut(s) 1188, 1220
BpmI CTGGAG 1 cut(s) 1204
Bpu10I CCTNAGC 1 cut(s) 1134
Bpu1102I GCTNAGC 1 cut(s) 370
BpuMI CCSGG 1 cut(s) 447
BsaAI YACGTR 2 cut(s) 433, 596
BsaHI GRCGYC 1 cut(s) 1323
BsaI GGTCTC 2 cut(s) 37, 1191
BsaJI CCNNGG 8 cut(s) 405, 445, 664, 705, 998, 1096, 1260, 1293
BsaWI WCCGGW 3 cut(s) 695, 711, 1244
BsaXI ACNNNNNCTCC 4 cut(s) 591, 621, 905, 935
Bsc4I CCNNNNNNNGG 5 cut(s) 476, 711, 895, 930, 1124
Bse118I RCCGGY 1 cut(s) 1319
Bse1I ACTGG 5 cut(s) 912, 1172, 1181, 1187, 1243
Bse3DI GCAATG 1 cut(s) 1224
BseAI TCCGGA 2 cut(s) 711, 1244
BseBI CCWGG 2 cut(s) 1097, 1261
BseDI CCNNGG 8 cut(s) 405, 445, 664, 705, 998, 1096, 1260, 1293
BseGI GGATG 2 cut(s) 660, 999
BseLI CCNNNNNNNGG 5 cut(s) 476, 711, 895, 930, 1124
BseMI GCAATG 1 cut(s) 1224
BseNI ACTGG 5 cut(s) 912, 1172, 1181, 1187, 1243
BseSI GKGCMC 1 cut(s) 612
BseXI GCAGC 2 cut(s) 364, 657
BsgI GTGCAG 1 cut(s) 840
Bsh1236I CGCG 2 cut(s) 417, 761
BshFI GGCC 5 cut(s) 511, 912, 1115, 1265, 1280
BsiHKAI GWGCWC 3 cut(s) 213, 612, 719
BsiSI CCGG 7 cut(s) 447, 477, 696, 712, 1245, 1320, 1336
BslFI GGGAC 4 cut(s) 156, 770, 947, 993
BslI CCNNNNNNNGG 5 cut(s) 476, 711, 895, 930, 1124
BsmAI GTCTC 2 cut(s) 37, 1191
BsmFI GGGAC 4 cut(s) 156, 770, 947, 993
BsmI GAATGC 1 cut(s) 258
BsnI GGCC 5 cut(s) 511, 912, 1115, 1265, 1280
Bso31I GGTCTC 2 cut(s) 37, 1191
Bsp1286I GDGCHC 4 cut(s) 213, 612, 719, 851
Bsp13I TCCGGA 2 cut(s) 711, 1244
Bsp143I GATC 5 cut(s) 457, 543, 776, 792, 1187
Bsp1720I GCTNAGC 1 cut(s) 370
Bsp19I CCATGG 3 cut(s) 664, 705, 1293
BspACI CCGC 7 cut(s) 29, 68, 361, 381, 411, 487, 749
BspANI GGCC 5 cut(s) 511, 912, 1115, 1265, 1280
BspEI TCCGGA 2 cut(s) 711, 1244
BspFNI CGCG 2 cut(s) 417, 761
BspLI GGNNCC 7 cut(s) 144, 778, 1116, 1216, 1243, 1279, 1303
BspMAI CTGCAG 1 cut(s) 109
BspMI ACCTGC 1 cut(s) 1209
BspPI GGATC 4 cut(s) 452, 771, 784, 800
BspTNI GGTCTC 2 cut(s) 37, 1191
BsrDI GCAATG 1 cut(s) 1224
BsrFI RCCGGY 1 cut(s) 1319
BsrI ACTGG 5 cut(s) 912, 1172, 1181, 1187, 1243
BssAI RCCGGY 1 cut(s) 1319
BssECI CCNNGG 8 cut(s) 405, 445, 664, 705, 998, 1096, 1260, 1293
BssMI GATC 5 cut(s) 457, 543, 776, 792, 1187
BssNI GRCGYC 1 cut(s) 1323
BssT1I CCWWGG 4 cut(s) 664, 705, 998, 1293
Bst2UI CCWGG 2 cut(s) 1097, 1261
Bst4CI ACNGT 1 cut(s) 917
BstACI GRCGYC 1 cut(s) 1323
BstAPI GCANNNNNTGC 1 cut(s) 1256
BstBAI YACGTR 2 cut(s) 433, 596
BstC8I GCNNGC 9 cut(s) 87, 91, 213, 365, 628, 719, 1252, 1315, 1321
BstDEI CTNAG 2 cut(s) 370, 1134
BstDSI CCRYGG 4 cut(s) 405, 664, 705, 1293
BstENI CCTNNNNNAGG 1 cut(s) 928
BstF5I GGATG 2 cut(s) 660, 999
BstFNI CGCG 2 cut(s) 417, 761
BstH2I RGCGCY 2 cut(s) 370, 1320
BstHHI GCGC 3 cut(s) 369, 399, 1319
BstKTI GATC 5 cut(s) 460, 546, 779, 795, 1190
BstMAI GTCTC 2 cut(s) 37, 1191
BstMBI GATC 5 cut(s) 457, 543, 776, 792, 1187
BstMWI GCNNNNNNNGC 4 cut(s) 26, 86, 623, 1256
BstNI CCWGG 2 cut(s) 1097, 1261
BstNSI RCATGY 1 cut(s) 630
BstSCI CCNGG 3 cut(s) 445, 1095, 1259
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 1 cut(s) 612
BstUI CGCG 2 cut(s) 417, 761
BstV1I GCAGC 2 cut(s) 364, 657
BstX2I RGATCY 1 cut(s) 776
BstXI CCANNNNNNTGG 1 cut(s) 1167
BstYI RGATCY 1 cut(s) 776
BsuRI GGCC 5 cut(s) 511, 912, 1115, 1265, 1280
BtgI CCRYGG 4 cut(s) 405, 664, 705, 1293
BtsCI GGATG 2 cut(s) 660, 999
BtsIMutI CAGTG 2 cut(s) 922, 1174
BveI ACCTGC 1 cut(s) 1209
Cac8I GCNNGC 9 cut(s) 87, 91, 213, 365, 628, 719, 1252, 1315, 1321
CaiI CAGNNNCTG 1 cut(s) 1238
CfoI GCGC 3 cut(s) 369, 399, 1319
Cfr10I RCCGGY 1 cut(s) 1319
Cfr13I GGNCC 6 cut(s) 143, 709, 910, 1114, 1263, 1278
CseI GACGC 3 cut(s) 423, 767, 1312
CspCI CAANNNNNGTGG 2 cut(s) 120, 155
CviAII CATG 9 cut(s) 253, 538, 627, 665, 680, 706, 844, 1022, 1294
DdeI CTNAG 2 cut(s) 370, 1134
DpnI GATC 5 cut(s) 459, 545, 778, 794, 1189
DpnII GATC 5 cut(s) 457, 543, 776, 792, 1187
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EciI GGCGGA 3 cut(s) 426, 476, 764
Ecl136II GAGCTC 1 cut(s) 717
Eco130I CCWWGG 4 cut(s) 664, 705, 998, 1293
Eco24I GRGCYC 2 cut(s) 719, 851
Eco31I GGTCTC 2 cut(s) 37, 1191
Eco32I GATATC 1 cut(s) 1109
Eco47I GGWCC 2 cut(s) 143, 709
Eco47III AGCGCT 1 cut(s) 368
Eco53kI GAGCTC 1 cut(s) 717
Eco72I CACGTG 1 cut(s) 596
EcoICRI GAGCTC 1 cut(s) 717
EcoNI CCTNNNNNAGG 1 cut(s) 928
EcoO109I RGGNCCY 1 cut(s) 1263
EcoRII CCWGG 2 cut(s) 1095, 1259
EcoRV GATATC 1 cut(s) 1109
EcoT14I CCWWGG 4 cut(s) 664, 705, 998, 1293
EcoT22I ATGCAT 1 cut(s) 632
EcoT38I GRGCYC 2 cut(s) 719, 851
ErhI CCWWGG 4 cut(s) 664, 705, 998, 1293
FaeI CATG 9 cut(s) 256, 541, 630, 668, 683, 709, 847, 1025, 1297
FaqI GGGAC 4 cut(s) 156, 770, 947, 993
FatI CATG 9 cut(s) 252, 537, 626, 664, 679, 705, 843, 1021, 1293
FauNDI CATATG 1 cut(s) 619
FbaI TGATCA 1 cut(s) 543
Fnu4HI GCNGC 3 cut(s) 353, 382, 671
FokI GGATG 2 cut(s) 647, 1006
FriOI GRGCYC 2 cut(s) 719, 851
Fsp4HI GCNGC 3 cut(s) 353, 382, 671
FspBI CTAG 3 cut(s) 77, 471, 1269
GlaI GCGC 3 cut(s) 368, 398, 1318
GluI GCNGC 3 cut(s) 353, 382, 671
GsuI CTGGAG 1 cut(s) 1204
HaeII RGCGCY 2 cut(s) 370, 1320
HaeIII GGCC 5 cut(s) 511, 912, 1115, 1265, 1280
HapII CCGG 7 cut(s) 447, 477, 696, 712, 1245, 1320, 1336
HgaI GACGC 3 cut(s) 423, 767, 1312
HhaI GCGC 3 cut(s) 369, 399, 1319
Hin1I GRCGYC 1 cut(s) 1323
Hin1II CATG 9 cut(s) 256, 541, 630, 668, 683, 709, 847, 1025, 1297
Hin6I GCGC 3 cut(s) 367, 397, 1317
HinP1I GCGC 3 cut(s) 367, 397, 1317
HincII GTYRAC 3 cut(s) 250, 466, 1156
HindII GTYRAC 3 cut(s) 250, 466, 1156
HindIII AAGCTT 2 cut(s) 198, 938
HinfI GANTC 6 cut(s) 152, 291, 313, 600, 637, 920
HpaI GTTAAC 1 cut(s) 1156
HpaII CCGG 7 cut(s) 447, 477, 696, 712, 1245, 1320, 1336
HphI GGTGA 6 cut(s) 448, 553, 874, 986, 1039, 1153
Hpy166II GTNNAC 6 cut(s) 250, 439, 466, 610, 1030, 1156
Hpy188I TCNGA 2 cut(s) 990, 1192
Hpy188III TCNNGA 6 cut(s) 227, 503, 580, 712, 1233, 1245
Hpy8I GTNNAC 6 cut(s) 250, 439, 466, 610, 1030, 1156
Hpy99I CGWCG 3 cut(s) 310, 422, 458
HpyAV CCTTC 1 cut(s) 1187
HpyCH4III ACNGT 1 cut(s) 917
HpyCH4IV ACGT 3 cut(s) 432, 595, 654
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 86, 623, 1256
HpyF3I CTNAG 2 cut(s) 370, 1134
HpySE526I ACGT 3 cut(s) 432, 595, 654
Hsp92I GRCGYC 1 cut(s) 1323
Hsp92II CATG 9 cut(s) 256, 541, 630, 668, 683, 709, 847, 1025, 1297
HspAI GCGC 3 cut(s) 367, 397, 1317
Kpn2I TCCGGA 2 cut(s) 711, 1244
KroI GCCGGC 1 cut(s) 1319
KroNI GCCGGC 1 cut(s) 1321
Ksp22I TGATCA 1 cut(s) 543
KspAI GTTAAC 1 cut(s) 1156
Kzo9I GATC 5 cut(s) 457, 543, 776, 792, 1187
LmnI GCTCC 7 cut(s) 167, 360, 714, 1209, 1214, 1247, 1307
Lsp1109I GCAGC 2 cut(s) 364, 657
LweI GCATC 4 cut(s) 580, 826, 984, 1238
MaeI CTAG 3 cut(s) 77, 471, 1269
MaeII ACGT 3 cut(s) 432, 595, 654
MaeIII GTNAC 7 cut(s) 51, 338, 533, 646, 917, 949, 1141
MalI GATC 5 cut(s) 459, 545, 778, 794, 1189
MboI GATC 5 cut(s) 457, 543, 776, 792, 1187
MboII GAAGA 4 cut(s) 65, 206, 291, 1281
MfeI CAATTG 3 cut(s) 318, 585, 954
MflI RGATCY 1 cut(s) 776
MhlI GDGCHC 4 cut(s) 213, 612, 719, 851
MluCI AATT 9 cut(s) 127, 234, 283, 318, 564, 585, 802, 954, 1086
MlyI GAGTC 3 cut(s) 161, 609, 914
MmeI TCCRAC 2 cut(s) 311, 894
Mph1103I ATGCAT 1 cut(s) 632
MreI CGCCGGCG 1 cut(s) 1319
MroI TCCGGA 2 cut(s) 711, 1244
MroNI GCCGGC 1 cut(s) 1319
MroXI GAANNNNTTC 2 cut(s) 575, 1240
MseI TTAA 4 cut(s) 126, 1089, 1155, 1354
MslI CAYNNNNRTG 3 cut(s) 678, 891, 972
MspI CCGG 7 cut(s) 447, 477, 696, 712, 1245, 1320, 1336
MspR9I CCNGG 3 cut(s) 447, 1097, 1261
MunI CAATTG 3 cut(s) 318, 585, 954
Mva1269I GAATGC 1 cut(s) 258
MvaI CCWGG 2 cut(s) 1097, 1261
MvnI CGCG 2 cut(s) 417, 761
MwoI GCNNNNNNNGC 4 cut(s) 26, 86, 623, 1256
NaeI GCCGGC 1 cut(s) 1321
NciI CCSGG 1 cut(s) 447
NcoI CCATGG 3 cut(s) 664, 705, 1293
NdeI CATATG 1 cut(s) 619
NdeII GATC 5 cut(s) 457, 543, 776, 792, 1187
NgoMIV GCCGGC 1 cut(s) 1319
NlaIII CATG 9 cut(s) 256, 541, 630, 668, 683, 709, 847, 1025, 1297
NlaIV GGNNCC 7 cut(s) 144, 778, 1116, 1216, 1243, 1279, 1303
NmuCI GTSAC 5 cut(s) 338, 533, 917, 949, 1141
NsiI ATGCAT 1 cut(s) 632
NspI RCATGY 1 cut(s) 630
PaeI GCATGC 1 cut(s) 630
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 459
PctI GAATGC 1 cut(s) 258
PdiI GCCGGC 1 cut(s) 1321
PdmI GAANNNNTTC 2 cut(s) 575, 1240
PfeI GAWTC 3 cut(s) 291, 313, 637
PkrI GCNGC 3 cut(s) 354, 383, 672
PleI GAGTC 3 cut(s) 160, 608, 914
PmaCI CACGTG 1 cut(s) 596
PmlI CACGTG 1 cut(s) 596
PpsI GAGTC 3 cut(s) 160, 608, 914
Ppu21I YACGTR 2 cut(s) 433, 596
Psp124BI GAGCTC 1 cut(s) 719
Psp6I CCWGG 2 cut(s) 1095, 1259
PspCI CACGTG 1 cut(s) 596
PspGI CCWGG 2 cut(s) 1095, 1259
PspN4I GGNNCC 7 cut(s) 144, 778, 1116, 1216, 1243, 1279, 1303
PspPI GGNCC 6 cut(s) 143, 709, 910, 1114, 1263, 1278
PsrI GAACNNNNNNTAC 2 cut(s) 1022, 1054
PstI CTGCAG 1 cut(s) 109
PstNI CAGNNNCTG 1 cut(s) 1238
PsuI RGATCY 1 cut(s) 776
RseI CAYNNNNRTG 3 cut(s) 678, 891, 972
SacI GAGCTC 1 cut(s) 719
SaqAI TTAA 4 cut(s) 126, 1089, 1155, 1354
SatI GCNGC 3 cut(s) 353, 382, 671
Sau3AI GATC 5 cut(s) 457, 543, 776, 792, 1187
Sau96I GGNCC 6 cut(s) 143, 709, 910, 1114, 1263, 1278
SchI GAGTC 3 cut(s) 161, 609, 914
ScrFI CCNGG 3 cut(s) 447, 1097, 1261
SduI GDGCHC 4 cut(s) 213, 612, 719, 851
SfaNI GCATC 4 cut(s) 580, 826, 984, 1238
SfcI CTRYAG 1 cut(s) 105
SgrAI CRCCGGYG 1 cut(s) 1319
SinI GGWCC 2 cut(s) 143, 709
SmiMI CAYNNNNRTG 3 cut(s) 678, 891, 972
SphI GCATGC 1 cut(s) 630
Sse9I AATT 9 cut(s) 127, 234, 283, 318, 564, 585, 802, 954, 1086
SsiI CCGC 7 cut(s) 29, 68, 361, 381, 411, 487, 749
SspMI CTAG 3 cut(s) 77, 471, 1269
SstI GAGCTC 1 cut(s) 719
StyD4I CCNGG 3 cut(s) 445, 1095, 1259
StyI CCWWGG 4 cut(s) 664, 705, 998, 1293
TaaI ACNGT 1 cut(s) 917
TaiI ACGT 3 cut(s) 435, 598, 657
TaqI TCGA 2 cut(s) 59, 795
TasI AATT 9 cut(s) 127, 234, 283, 318, 564, 585, 802, 954, 1086
TauI GCSGC 1 cut(s) 384
TfiI GAWTC 3 cut(s) 291, 313, 637
Tru1I TTAA 4 cut(s) 126, 1089, 1155, 1354
Tru9I TTAA 4 cut(s) 126, 1089, 1155, 1354
TscAI CASTG 2 cut(s) 922, 1181
TseFI GTSAC 5 cut(s) 338, 533, 917, 949, 1141
TseI GCWGC 2 cut(s) 352, 670
Tsp45I GTSAC 5 cut(s) 338, 533, 917, 949, 1141
TspDTI ATGAA 3 cut(s) 246, 584, 1213
TspRI CASTG 2 cut(s) 922, 1181
VneI GTGCAC 1 cut(s) 608
VpaK11BI GGWCC 2 cut(s) 143, 709
XagI CCTNNNNNAGG 1 cut(s) 928
XapI RAATTY 1 cut(s) 283
XceI RCATGY 1 cut(s) 630
XmnI GAANNNNTTC 2 cut(s) 575, 1240
XspI CTAG 3 cut(s) 77, 471, 1269
Zsp2I ATGCAT 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.