pycom10g14600

Kinase-like protein TMKL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015402
Physical Location & Seq
Reverse (-)
17962344 .. 17965283
2940 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g14600.2

Sequence Viewer

Length: 2130 bp
ATGCTCCAATCCAACGTTAACACTCTTGTCAGCTTTTCCAAAAATAAGAGAGCTTCTTTGAACCGCCCACCACCTACAATACCACCTTTCCTCTCTGCTCCTTCCATGGAGGTTTCGAAACGTTTCTCTCTCTACATTTTCCTCCTCTGCTTCCTCCTACCCTTGAACAGCAAACCCACCGACTCTGTTTCTGCAGATGTCGAGCTCCTCCTGGGAAAAATCAAAGCTTCACTGCAAGGTAACTCTCAGAACTTGCTCTTATCTTCCTGGAACTCCTCTGTGCCTCTCTGCCAATGGAGGGGACTCAAATGGGTTTTCTCCAACGGCTCTATTTTGCTCTGCACCGACCTATCCTCCCCTCAGTGGACCAACTTGTCACTCTCCCGAGACCCAGCTCTCCACCTTTTTTCCCTTCAGCTCCCATCTGCCAATCTGTCTGGTTCTCTTCCCAGAGAGCTGGGGGAGTTCTCTATGCTCCAAAGCTTGTACCTTAACATAAATTTCCTCACTGGGACCGTCCCATTGGAGCTTGGGTACAGCTCTACCCTCTCTGACATCGATTTGGGAAGCAATTTGTTGAATGGGGTTTTACCGCCGTCGATCTGGAACTTGTGTGACAATCTGGTTTCCCTTAGGGTTCATGCGAATTCGCTGTTCGGTTCGGTTCCTGAACCTGCATTGCGAAACTCTAGCTGCAAGAATTTGCAGTTTCTTGATTTTGGTGGGAACAAGTTTTCTGGGAATTTCCCAGAATTTGTAACCCAGTTTGGTGGGCTTAAAGAGATCGATCTTGGAAGTAACATGTTTTCGGGTCTCATTCCTGGGGGTTTAGTGCAGTTAAACCTCGAAAAATTGAACCTTTCGCATAATAATTTTAGTGGGGTGTTGCCTGTTTTTGGTGGATCAAAGTTTGGTGTGGAGGCTTTTGAGGGTAACAGCCCTGGACTTTGTGGAGCGCCTTTGAAAAGTTGCAGTGGAAGCTCTGGGTTGAGTCCCGGTGCAATTGCCGGCATTGTTATTGGTTTGATGACTGCAACTGTGGTTTTGGCTTCATTGTGTATTGGGTATGTGCAAAACAAAAAGAAAAACAGCACGGAAGAGAGTGAAGATGAAATAGAGGAAGAAGAAGATGAGGAAAGTGGGGGCGGAGGCGGTGGCGGTGGTGATCAAGGAAGGCTTATTTTGTTTCAGGGCGGCGAGCATTTGACGTTGGAGGATGTGTTGAATGCAACAGGACAAGTGTTGGAGAAGACCAGCTATGGGACTGTGTACAAGGCAAAGCTCTCAGATGGAGGGACCATCACTTTGAGGCTGTTGAAGGAAGGTAGTTGCAAGGATGGAAGCTCTTGTCTTCCTGTGGTGAAGCAACTGGGGAGGATTCGCCATGAGAATTTGATTCCGTTGAGAGCTTTCTATCAGGGGAAGAGAGGGGAGAAGCTTCTGATATATGACCATCTTCCTCACAGAAGCCTTCATGATCTTTTACATGAAGCCAGAGCAGGGAAACTTGTGTTGAACTGGGCTCGGCGACACAAGATTGCACTGGGAATAGCTCGAGGACTAGCATATCTTCATACTGGTCTTGAAACACCTATCACTCATGGGAGTGTAAAATCTAAGAATGTGCTAGTGGATGAGTTTTCTGTAGCTAGGCTCACAGAATTTGGGCTTGACAAGCTAATGATCCCTACCGTGGCTGATGAAATGGTGGCGGTTGCAAAAATTGACGGTTATAAGGCACCAGAGCTTCAAAAGATGAAGAAATGTAATTCAAGGACAGATGTTTATGCATTTGGAATTATTCTGTTGGAAATTTTGATAGGCAAGAAGCCTGGTAAAACCGGGAGGAGTGGTGAATTTGTGGATTTGCCTTTGATGGTGAAAGTTGCAGTCTTGGAGGAGACGACACTGGAGGTTTTCGACGTGGAGGTACTGAAGGGGGTGAGGAGTGCAATGGAAGAAGGTCTGGTTCAGGCATTGAAACTTGCAATGGGTTGCTGTGCGCCGGTTGCTTCGGTTAGACCCTCCATGGATGAAGTTGTGAGGCAGTTGGAGGAGAACAGGCCAAGGAATAGGTCTACTTTGTACAGCCCAACAGAGACAAGGAGTGAGATTGGTACCCCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

710

Amino Acids

76.73

Weight (kDa)

6.66

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24660
fragaria_vesca FvH4_2g19550
malus_domestica MD05G1179600.v1.1 MD10G1169200.v1.1
prunus_persica Prupe.8G206900_v2.0.a1
pyrus_communis pycom05g16610 pycom10g14600
rosa_chinensis RchiOBHm_Chr6g0285321
rosa_laevigata RLG00000012654
rosa_multiflora Rmu_sc0011958.1_g000002
rosa_roxburghii Rroxscaffold_7G00183360
rosa_rugosa Rorug06G0173500
rosa_samantha Rh6BG285000 Rh6CG286500 Rh6DG279500
rosa_wichuraiana Rw6G024460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1734
AasI GACNNNNNNGTC 1 cut(s) 373
Acc36I ACCTGC 1 cut(s) 682
Acc65I GGTACC 1 cut(s) 2117
AccB1I GGYRCC 2 cut(s) 1738, 2117
AccI GTMKAC 1 cut(s) 2078
AciI CCGC 7 cut(s) 64, 593, 1146, 1152, 1158, 1194, 1712
AclI AACGTT 2 cut(s) 15, 121
AclWI GGATC 2 cut(s) 910, 1678
AcsI RAATTY 9 cut(s) 499, 646, 700, 742, 752, 1390, 1661, 1812, 1856
AcuI CTGAAG 2 cut(s) 398, 1955
AfaI GTAC 6 cut(s) 488, 536, 1271, 1932, 2087, 2119
AfiI CCNNNNNNNGG 6 cut(s) 298, 363, 896, 1260, 1500, 1693
AflIII ACRYGT 1 cut(s) 801
AjiI CACGTC 1 cut(s) 1924
AjnI CCWGG 5 cut(s) 210, 266, 820, 940, 1831
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 4 cut(s) 381, 818, 1895, 2093
AlwI GGATC 2 cut(s) 910, 1678
Ama87I CYCGRG 2 cut(s) 384, 1554
AoxI GGCC 1 cut(s) 2063
ApeKI GCWGC 1 cut(s) 693
ApoI RAATTY 9 cut(s) 499, 646, 700, 742, 752, 1390, 1661, 1812, 1856
ArsI GACNNNNNNTTYG 2 cut(s) 1288, 1320
Asp700I GAANNNNTTC 1 cut(s) 122
Asp718I GGTACC 1 cut(s) 2117
AspLEI GCGC 2 cut(s) 958, 2005
AspS9I GGNCC 3 cut(s) 366, 513, 1296
AsuC2I CCSGG 2 cut(s) 996, 1843
AsuHPI GGTGA 5 cut(s) 1175, 1372, 1865, 1891, 1954
AsuII TTCGAA 1 cut(s) 116
AvaI CYCGRG 2 cut(s) 384, 1554
AvaII GGWCC 3 cut(s) 366, 513, 1296
AxyI CCTNAGG 1 cut(s) 632
BanI GGYRCC 2 cut(s) 1738, 2117
BanII GRGCYC 2 cut(s) 207, 1525
BbsI GAAGAC 2 cut(s) 1256, 1343
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 680
BccI CCATC 6 cut(s) 430, 1283, 1307, 1331, 1461, 1870
BceAI ACGGC 2 cut(s) 340, 580
BciT130I CCWGG 5 cut(s) 212, 268, 822, 942, 1833
BclI TGATCA 1 cut(s) 1165
BcnI CCSGG 2 cut(s) 996, 1843
BcoDI GTCTC 4 cut(s) 381, 818, 1895, 2093
BfaI CTAG 4 cut(s) 690, 1562, 1628, 1650
BfmI CTRYAG 2 cut(s) 192, 1644
BfoI RGCGCY 1 cut(s) 959
BfuAI ACCTGC 1 cut(s) 682
BisI GCNGC 2 cut(s) 694, 1195
BlsI GCNGC 2 cut(s) 695, 1196
Bme1390I CCNGG 7 cut(s) 212, 268, 822, 942, 996, 1833, 1843
Bme18I GGWCC 3 cut(s) 366, 513, 1296
BmeT110I CYCGRG 2 cut(s) 384, 1554
BmgBI CACGTC 1 cut(s) 1924
BmgT120I GGNCC 3 cut(s) 366, 513, 1296
BmiI GGNNCC 5 cut(s) 514, 666, 1297, 1740, 2119
BmrFI CCNGG 7 cut(s) 212, 268, 822, 942, 996, 1833, 1843
BmrI ACTGGG 5 cut(s) 519, 757, 1379, 1528, 1553
BmuI ACTGGG 5 cut(s) 519, 757, 1379, 1528, 1553
BpiI GAAGAC 2 cut(s) 1256, 1343
BpmI CTGGAG 1 cut(s) 1931
Bpu14I TTCGAA 1 cut(s) 116
BpuMI CCSGG 2 cut(s) 996, 1843
Bsa29I ATCGAT 2 cut(s) 558, 786
BsaI GGTCTC 2 cut(s) 381, 818
BsaJI CCNNGG 7 cut(s) 105, 211, 821, 940, 1692, 2028, 2066
BsaXI ACNNNNNCTCC 8 cut(s) 338, 368, 518, 548, 1205, 1235, 1837, 1867
Bsc4I CCNNNNNNNGG 6 cut(s) 298, 363, 896, 1260, 1500, 1693
Bse118I RCCGGY 2 cut(s) 1007, 2005
Bse1I ACTGG 7 cut(s) 514, 763, 1374, 1523, 1548, 1582, 1914
Bse21I CCTNAGG 1 cut(s) 632
Bse3DI GCAATG 3 cut(s) 677, 1959, 1995
BseBI CCWGG 5 cut(s) 212, 268, 822, 942, 1833
BseCI ATCGAT 2 cut(s) 558, 786
BseDI CCNNGG 7 cut(s) 105, 211, 821, 940, 1692, 2028, 2066
BseGI GGATG 4 cut(s) 1222, 1342, 1639, 2038
BseLI CCNNNNNNNGG 6 cut(s) 298, 363, 896, 1260, 1500, 1693
BseMI GCAATG 3 cut(s) 677, 1959, 1995
BseMII CTCAG 3 cut(s) 260, 374, 1299
BseNI ACTGG 7 cut(s) 514, 763, 1374, 1523, 1548, 1582, 1914
BseRI GAGGAG 7 cut(s) 134, 197, 265, 1861, 1913, 1960, 2069
BseXI GCAGC 1 cut(s) 680
BseYI CCCAGC 2 cut(s) 391, 457
BsgI GTGCAG 2 cut(s) 325, 854
BshFI GGCC 1 cut(s) 2065
BshNI GGYRCC 2 cut(s) 1738, 2117
BshVI ATCGAT 2 cut(s) 558, 786
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 2 cut(s) 384, 1554
BsiSI CCGG 4 cut(s) 996, 1008, 1842, 2006
BslFI GGGAC 6 cut(s) 315, 503, 526, 978, 1276, 1309
BslI CCNNNNNNNGG 6 cut(s) 298, 363, 896, 1260, 1500, 1693
BsmAI GTCTC 4 cut(s) 381, 818, 1895, 2093
BsmBI CGTCTC 1 cut(s) 1895
BsmFI GGGAC 6 cut(s) 315, 503, 526, 978, 1276, 1309
BsmI GAATGC 1 cut(s) 1231
BsnI GGCC 1 cut(s) 2065
Bso31I GGTCTC 2 cut(s) 381, 818
BsoBI CYCGRG 2 cut(s) 384, 1554
Bsp119I TTCGAA 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 207, 1525
Bsp1407I TGTACA 2 cut(s) 1269, 2085
Bsp143I GATC 7 cut(s) 600, 783, 787, 902, 1165, 1477, 1683
Bsp19I CCATGG 2 cut(s) 105, 2028
BspACI CCGC 7 cut(s) 64, 593, 1146, 1152, 1158, 1194, 1712
BspANI GGCC 1 cut(s) 2065
BspCNI CTCAG 3 cut(s) 259, 373, 1298
BspDI ATCGAT 2 cut(s) 558, 786
BspHI TCATGA 1 cut(s) 1474
BspLI GGNNCC 5 cut(s) 514, 666, 1297, 1740, 2119
BspMAI CTGCAG 1 cut(s) 196
BspMI ACCTGC 1 cut(s) 682
BspPI GGATC 2 cut(s) 910, 1678
BspT104I TTCGAA 1 cut(s) 116
BspT107I GGYRCC 2 cut(s) 1738, 2117
BspTNI GGTCTC 2 cut(s) 381, 818
BsrDI GCAATG 3 cut(s) 677, 1959, 1995
BsrFI RCCGGY 2 cut(s) 1007, 2005
BsrGI TGTACA 2 cut(s) 1269, 2085
BsrI ACTGG 7 cut(s) 514, 763, 1374, 1523, 1548, 1582, 1914
BssAI RCCGGY 2 cut(s) 1007, 2005
BssECI CCNNGG 7 cut(s) 105, 211, 821, 940, 1692, 2028, 2066
BssMI GATC 7 cut(s) 600, 783, 787, 902, 1165, 1477, 1683
BssT1I CCWWGG 3 cut(s) 105, 2028, 2066
Bst2UI CCWGG 5 cut(s) 212, 268, 822, 942, 1833
Bst4CI ACNGT 5 cut(s) 517, 1039, 1267, 1693, 1730
Bst6I CTCTTC 3 cut(s) 450, 1092, 1418
BstAUI TGTACA 2 cut(s) 1269, 2085
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 1009, 1199
BstDEI CTNAG 5 cut(s) 246, 360, 632, 1285, 1617
BstDSI CCRYGG 3 cut(s) 105, 1692, 2028
BstF5I GGATG 4 cut(s) 1222, 1342, 1639, 2038
BstH2I RGCGCY 1 cut(s) 959
BstHHI GCGC 2 cut(s) 958, 2005
BstKTI GATC 7 cut(s) 603, 786, 790, 905, 1168, 1480, 1686
BstMAI GTCTC 4 cut(s) 381, 818, 1895, 2093
BstMBI GATC 7 cut(s) 600, 783, 787, 902, 1165, 1477, 1683
BstMWI GCNNNNNNNGC 3 cut(s) 978, 1675, 2009
BstNI CCWGG 5 cut(s) 212, 268, 822, 942, 1833
BstNSI RCATGY 1 cut(s) 805
BstSCI CCNGG 7 cut(s) 210, 266, 820, 940, 994, 1831, 1841
BstSFI CTRYAG 2 cut(s) 192, 1644
BstV1I GCAGC 1 cut(s) 680
BstV2I GAAGAC 2 cut(s) 1256, 1343
BstXI CCANNNNNNTGG 2 cut(s) 457, 770
Bsu15I ATCGAT 2 cut(s) 558, 786
Bsu36I CCTNAGG 1 cut(s) 632
BsuRI GGCC 1 cut(s) 2065
BsuTUI ATCGAT 2 cut(s) 558, 786
BtgI CCRYGG 3 cut(s) 105, 1692, 2028
BtrI CACGTC 1 cut(s) 1924
BtsCI GGATG 4 cut(s) 1222, 1342, 1639, 2038
BtsI GCAGTG 2 cut(s) 230, 979
BtsIMutI CAGTG 6 cut(s) 230, 368, 507, 979, 1541, 1907
BveI ACCTGC 1 cut(s) 682
Cac8I GCNNGC 2 cut(s) 1009, 1199
CciI TCATGA 1 cut(s) 1474
CfoI GCGC 2 cut(s) 958, 2005
Cfr10I RCCGGY 2 cut(s) 1007, 2005
Cfr13I GGNCC 3 cut(s) 366, 513, 1296
ClaI ATCGAT 2 cut(s) 558, 786
Csp6I GTAC 6 cut(s) 487, 535, 1270, 1931, 2086, 2118
CviAII CATG 8 cut(s) 106, 641, 802, 1385, 1475, 1487, 1601, 2029
CviQI GTAC 6 cut(s) 487, 535, 1270, 1931, 2086, 2118
DdeI CTNAG 5 cut(s) 246, 360, 632, 1285, 1617
DpnI GATC 7 cut(s) 602, 785, 789, 904, 1167, 1479, 1685
DpnII GATC 7 cut(s) 600, 783, 787, 902, 1165, 1477, 1683
DrdI GACNNNNNNGTC 1 cut(s) 373
DseDI GACNNNNNNGTC 1 cut(s) 373
Eam1104I CTCTTC 3 cut(s) 450, 1092, 1418
EarI CTCTTC 3 cut(s) 450, 1092, 1418
EciI GGCGGA 1 cut(s) 1161
Ecl136II GAGCTC 1 cut(s) 205
Eco130I CCWWGG 3 cut(s) 105, 2028, 2066
Eco24I GRGCYC 2 cut(s) 207, 1525
Eco31I GGTCTC 2 cut(s) 381, 818
Eco47I GGWCC 3 cut(s) 366, 513, 1296
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 2 cut(s) 398, 1955
Eco81I CCTNAGG 1 cut(s) 632
Eco88I CYCGRG 2 cut(s) 384, 1554
EcoICRI GAGCTC 1 cut(s) 205
EcoRI GAATTC 1 cut(s) 646
EcoRII CCWGG 5 cut(s) 210, 266, 820, 940, 1831
EcoT14I CCWWGG 3 cut(s) 105, 2028, 2066
EcoT22I ATGCAT 1 cut(s) 1792
EcoT38I GRGCYC 2 cut(s) 207, 1525
ErhI CCWWGG 3 cut(s) 105, 2028, 2066
Esp3I CGTCTC 1 cut(s) 1895
FaeI CATG 8 cut(s) 109, 644, 805, 1388, 1478, 1490, 1604, 2032
FalI AAGNNNNNCTT 2 cut(s) 1161, 1193
FaqI GGGAC 6 cut(s) 315, 503, 526, 978, 1276, 1309
FatI CATG 8 cut(s) 105, 640, 801, 1384, 1474, 1486, 1600, 2028
FbaI TGATCA 1 cut(s) 1165
FblI GTMKAC 1 cut(s) 2078
Fnu4HI GCNGC 2 cut(s) 694, 1195
FokI GGATG 4 cut(s) 1229, 1349, 1646, 2045
FriOI GRGCYC 2 cut(s) 207, 1525
Fsp4HI GCNGC 2 cut(s) 694, 1195
FspBI CTAG 4 cut(s) 690, 1562, 1628, 1650
GlaI GCGC 2 cut(s) 957, 2004
GluI GCNGC 2 cut(s) 694, 1195
GsaI CCCAGC 2 cut(s) 395, 461
GsuI CTGGAG 1 cut(s) 1931
HaeII RGCGCY 1 cut(s) 959
HaeIII GGCC 1 cut(s) 2065
HapII CCGG 4 cut(s) 996, 1008, 1842, 2006
HhaI GCGC 2 cut(s) 958, 2005
Hin1II CATG 8 cut(s) 109, 644, 805, 1388, 1478, 1490, 1604, 2032
Hin6I GCGC 2 cut(s) 956, 2003
HinP1I GCGC 2 cut(s) 956, 2003
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HindIII AAGCTT 3 cut(s) 225, 481, 1436
HinfI GANTC 5 cut(s) 182, 303, 991, 1378, 1396
HpaI GTTAAC 1 cut(s) 19
HpaII CCGG 4 cut(s) 996, 1008, 1842, 2006
HphI GGTGA 5 cut(s) 1175, 1372, 1865, 1891, 1954
Hpy166II GTNNAC 4 cut(s) 19, 366, 1270, 2079
Hpy188I TCNGA 4 cut(s) 249, 553, 1288, 1443
Hpy188III TCNNGA 6 cut(s) 384, 604, 668, 713, 1475, 1583
Hpy8I GTNNAC 4 cut(s) 19, 366, 1270, 2079
Hpy99I CGWCG 2 cut(s) 601, 1925
HpyAV CCTTC 8 cut(s) 111, 422, 1167, 1312, 1316, 1481, 1930, 1955
HpyCH4III ACNGT 5 cut(s) 517, 1039, 1267, 1693, 1730
HpyCH4IV ACGT 4 cut(s) 15, 121, 1208, 1923
HpyF10VI GCNNNNNNNGC 3 cut(s) 978, 1675, 2009
HpyF3I CTNAG 5 cut(s) 246, 360, 632, 1285, 1617
HpySE526I ACGT 4 cut(s) 15, 121, 1208, 1923
Hsp92II CATG 8 cut(s) 109, 644, 805, 1388, 1478, 1490, 1604, 2032
HspAI GCGC 2 cut(s) 956, 2003
KpnI GGTACC 1 cut(s) 2121
KroI GCCGGC 1 cut(s) 1007
KroNI GCCGGC 1 cut(s) 1009
Ksp22I TGATCA 1 cut(s) 1165
KspAI GTTAAC 1 cut(s) 19
Kzo9I GATC 7 cut(s) 600, 783, 787, 902, 1165, 1477, 1683
LmnI GCTCC 7 cut(s) 9, 103, 210, 423, 480, 526, 953
Lsp1109I GCAGC 1 cut(s) 680
MaeI CTAG 4 cut(s) 690, 1562, 1628, 1650
MaeII ACGT 4 cut(s) 15, 121, 1208, 1923
MaeIII GTNAC 6 cut(s) 239, 375, 614, 757, 797, 932
MalI GATC 7 cut(s) 602, 785, 789, 904, 1167, 1479, 1685
MboI GATC 7 cut(s) 600, 783, 787, 902, 1165, 1477, 1683
MfeI CAATTG 1 cut(s) 1002
MhlI GDGCHC 2 cut(s) 207, 1525
MlyI GAGTC 3 cut(s) 176, 297, 1000
MmeI TCCRAC 6 cut(s) 36, 345, 1191, 1224, 1788, 2031
Mph1103I ATGCAT 1 cut(s) 1792
MroNI GCCGGC 1 cut(s) 1007
MroXI GAANNNNTTC 1 cut(s) 122
MseI TTAA 4 cut(s) 18, 492, 777, 839
MslI CAYNNNNRTG 1 cut(s) 1605
MspI CCGG 4 cut(s) 996, 1008, 1842, 2006
MspR9I CCNGG 7 cut(s) 212, 268, 822, 942, 996, 1833, 1843
MunI CAATTG 1 cut(s) 1002
Mva1269I GAATGC 1 cut(s) 1231
MvaI CCWGG 5 cut(s) 212, 268, 822, 942, 1833
MwoI GCNNNNNNNGC 3 cut(s) 978, 1675, 2009
NaeI GCCGGC 1 cut(s) 1009
NciI CCSGG 2 cut(s) 996, 1843
NcoI CCATGG 2 cut(s) 105, 2028
NdeII GATC 7 cut(s) 600, 783, 787, 902, 1165, 1477, 1683
NgoMIV GCCGGC 1 cut(s) 1007
NlaIII CATG 8 cut(s) 109, 644, 805, 1388, 1478, 1490, 1604, 2032
NlaIV GGNNCC 5 cut(s) 514, 666, 1297, 1740, 2119
NmeAIII GCCGAG 1 cut(s) 1504
NmuCI GTSAC 2 cut(s) 375, 614
NsiI ATGCAT 1 cut(s) 1792
NspI RCATGY 1 cut(s) 805
NspV TTCGAA 1 cut(s) 116
PaeR7I CTCGAG 1 cut(s) 1554
PagI TCATGA 1 cut(s) 1474
PciI ACATGT 1 cut(s) 801
PctI GAATGC 1 cut(s) 1231
PdiI GCCGGC 1 cut(s) 1009
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 2 cut(s) 1378, 1396
PfoI TCCNGGA 1 cut(s) 266
PkrI GCNGC 2 cut(s) 695, 1196
PleI GAGTC 3 cut(s) 176, 297, 999
PpsI GAGTC 3 cut(s) 176, 297, 999
PscI ACATGT 1 cut(s) 801
PsiI TTATAA 1 cut(s) 1734
Psp124BI GAGCTC 1 cut(s) 207
Psp1406I AACGTT 2 cut(s) 15, 121
Psp6I CCWGG 5 cut(s) 210, 266, 820, 940, 1831
PspFI CCCAGC 2 cut(s) 391, 457
PspGI CCWGG 5 cut(s) 210, 266, 820, 940, 1831
PspN4I GGNNCC 5 cut(s) 514, 666, 1297, 1740, 2119
PspPI GGNCC 3 cut(s) 366, 513, 1296
PspXI VCTCGAGB 1 cut(s) 1554
PstI CTGCAG 1 cut(s) 196
RsaI GTAC 6 cut(s) 488, 536, 1271, 1932, 2087, 2119
RsaNI GTAC 6 cut(s) 487, 535, 1270, 1931, 2086, 2118
RseI CAYNNNNRTG 1 cut(s) 1605
SacI GAGCTC 1 cut(s) 207
SaqAI TTAA 4 cut(s) 18, 492, 777, 839
SatI GCNGC 2 cut(s) 694, 1195
Sau3AI GATC 7 cut(s) 600, 783, 787, 902, 1165, 1477, 1683
Sau96I GGNCC 3 cut(s) 366, 513, 1296
SchI GAGTC 3 cut(s) 176, 297, 1000
ScrFI CCNGG 7 cut(s) 212, 268, 822, 942, 996, 1833, 1843
SduI GDGCHC 2 cut(s) 207, 1525
SfcI CTRYAG 2 cut(s) 192, 1644
Sfr274I CTCGAG 1 cut(s) 1554
SfuI TTCGAA 1 cut(s) 116
SinI GGWCC 3 cut(s) 366, 513, 1296
SlaI CTCGAG 1 cut(s) 1554
SmiMI CAYNNNNRTG 1 cut(s) 1605
SmlI CTYRAG 1 cut(s) 1554
SmoI CTYRAG 1 cut(s) 1554
SsiI CCGC 7 cut(s) 64, 593, 1146, 1152, 1158, 1194, 1712
SspMI CTAG 4 cut(s) 690, 1562, 1628, 1650
SstI GAGCTC 1 cut(s) 207
StyD4I CCNGG 7 cut(s) 210, 266, 820, 940, 994, 1831, 1841
StyI CCWWGG 3 cut(s) 105, 2028, 2066
TaaI ACNGT 5 cut(s) 517, 1039, 1267, 1693, 1730
TaiI ACGT 4 cut(s) 18, 124, 1211, 1926
TaqI TCGA 8 cut(s) 116, 201, 558, 599, 786, 846, 1555, 1920
TatI WGTACW 2 cut(s) 1269, 2085
TauI GCSGC 1 cut(s) 1197
TfiI GAWTC 2 cut(s) 1378, 1396
Tru1I TTAA 4 cut(s) 18, 492, 777, 839
Tru9I TTAA 4 cut(s) 18, 492, 777, 839
TscAI CASTG 6 cut(s) 237, 368, 514, 979, 1548, 1914
TseFI GTSAC 2 cut(s) 375, 614
TseI GCWGC 1 cut(s) 693
Tsp45I GTSAC 2 cut(s) 375, 614
TspDTI ATGAA 9 cut(s) 629, 1041, 1125, 1463, 1503, 1562, 1716, 1772, 2049
TspGWI ACGGA 2 cut(s) 1109, 1389
TspRI CASTG 6 cut(s) 237, 368, 514, 979, 1548, 1914
VpaK11BI GGWCC 3 cut(s) 366, 513, 1296
XapI RAATTY 9 cut(s) 499, 646, 700, 742, 752, 1390, 1661, 1812, 1856
XceI RCATGY 1 cut(s) 805
XhoI CTCGAG 1 cut(s) 1554
XmiI GTMKAC 1 cut(s) 2078
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 4 cut(s) 690, 1562, 1628, 1650
Zsp2I ATGCAT 1 cut(s) 1792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.