MD05G1188700.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015880
Physical Location & Seq
Forward (+)
31650368 .. 31653723
3356 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1188700.v1.1.491

Sequence Viewer

Length: 3045 bp
ATGCAGTTGAGGAAAACCCAATTGCTGATCTTCTTATTTTTGTGCTGTTCTTCATTTGGCATTGCAGCTGTGGCCAATGACGAAGTTTCTGCACTGCTTTCGATGAAAGCAGGCCTCATTGATCCACTAAACAGCCTCAAAGACTGGAACTTACCGGAAAATGGAGCTCACTGCAATTGGACAGGCGTATGGTGCAACACTGAGGGGCACGTAGAGAAGCTTGATCTCTCCCACATGAACCTCAGCGGCCCGGTATCCGGCGACATCCAACGTCTCCGAAGCCTCACTTCTCTCAACCTCTGCTGCAGTGGCTTCTCCTCATCACTGCCAAAATCCATCTCAAATCTCACAGCATTGAAAACTTTTGATGTGAGTCAAAACTCACTTGTTGGTGAGTTTCCATGGGGATTCGGAAGTGCTGGAGGATTGACGGAACTCAACGCTTCGAGTAATAATTTTTCGGGGTTTCTTCCTGAGGATCTCGGCAATGCCACAATGCTGGAGACTCTGGATCTCAGAGGGAACTTTTTCGGGGGCTCGATTCCAAAGTCATTCAAGAACTTGCAGAAGCTCAAGTTTCTTGGGCTTTCCGGCAACAGTCTTACCGGGGAAATCCCCGCTGAGTTTGGAGAGCTGTCCTCATTGGAAGCTATGATCCTTGGGTACAATGAGTTTGAAGGTGGGATTCCAATGGAGTTTGGGAATCTTACCAACCTGAAGTATCTTGATTTGGCAGTTGGGAATCTTAGTGGTGAGATTCCAGCTGAGTTGGGGAGGCTTCAGTTTCTTGAGACTGTGTTCTTATACAAGAACAATTTTGAAGGCAAAATTCCAGCTGAAATTGGCGCCATTTCTTCGCTGAAATTGCTGGATCTCTCTGATAATATGTTATCGGGGGAAATCCCAGCTGAGATTGGGGAGCTGAAGAATTTGCAGCTTTTGAATGTGATGAGTAATCAGCTGTCAGGTTCAGTTCCATTCGGAATTGGAAGTTTGAGTCAGTTAAGTGTTCTTGAGTTATGGAACAATTCGTTTTCAGGTCCTTTGCCTAGCGATCTTGGCAAAAATGCCCCATTGCAGTGGTTGGACATCTCATCCAACTCATTTTCCGGTGAGCTTCCATCCACATTGTGCAACAAGGGCAACCTCACCAAGCTCATCCTCTTCAACAATGCCCTCACAGGTCCAATTCCGGCGAGCCTATCCACGTGCCTTTCGCTTGTTCGTGTTCGAATGCAGAACAATTTTCTTTCCGGAACAATCCCAGTTGGGCTTGGCAAGCTTGAGAGGCTTCAGAGGTTAGAATTAGCAAACAACAATCTCACTGGTGCAATCCCAGATGATCTTTCTTCTTCTACTTCACTCTCTTTCATTGATGTCTCTCGAAACCGCCTCCATTCTTCTCTGCCTTCCACCATTCTCTCTGCACCAAGCTTGCAAACCCTGATGGCCTCGAATAATGAATTGGTCGGCAAAATCCCAGATCAATTCCAGGACAGCCCTTCACTTTCGGTGCTTGATCTCTCATCAAACCATTTCTCAGGAACCATCCCAGCAAGCATTGCTTCATGTGAGAGATTGGTAAGCTTGAATCTGAGGAACAACCAATTGAACGGAGACATCCCGAAATCAATTGCCATGATGCCCACATTGTCCATTCTTGATCTGTCCAACAACTCTCTCACTGGTGGCATACCCGAAAATTTCGGAATCTCACCAGCCTTGGAGACGCTCAATGTCTCATTCAACAAGCTAGAGGGTCCTGTCCCAGAAAATGGTGTGCTGAGAAGAATAAACCCGAGTGATCTTGTGGGCAATGCCGGACTCTGTGGCAGTGTCCTCCCTCCGTGCATGCGAAATCCAGTGCTGGCATCGAGGCATAGGAATGTGCACACAAGGAACATTGTTTCAGGATGGGTGATTGGGATCTCATCAGTTTTAGCAGTTGGACTCGCACTTTTCAGTGCTCGAACTCTATACAAGAGGTGGTACTCGAATGGGAGTTGCTTCGGAGACAGTTTCGAAGTTGGCAATGGAGAGTGGCCATGGAGACTGATGGCATTCCAGAGGCTTGGTTTCACAAGTACTGACATCCTGGCTTGTGTGAAGGAATCAAATGTGATCGGAATGGGAGCCACCGGGGTTGTGTACAAGGCAGAGATATCAAGATCAAACACAGTTGTGGCAGTTAAAAAGTTGTGGAGACCGGCAACAGACATTGAAACTGGAAGCAGTGACGATCTAGTTGGGGAAGTGAATCTGTTGGGAAGGCTAAGGCATCGAAACATAGTTCGATTGTTGGGATTTTTGAACAATGATACGAATTTGATGATCATATATGAGTTTATGCCCAATGGCAGCTTAGGAGAAACCTTGCATGGCAAGCAAGCAGGGAGATTGCTTGTAGATTGGGTTTCAAGGTACAACATAGCAGTTGGAGTTGCACAAGGTCTTTCCTATCTCCACCATGATTGTCACCCACCAGTCATCCATAGAGACATCAAGTCCAATAACATATTGCTTGATGCAAACCTCGACGCAAGGATTGCGGATTTCGGGTTGGCACGGATGATGGTTCGGAAAAACGAGACAGTGTCAATGGTGGCTGGATCATACGGTTACATAGCCCCTGAATATGGATACACATTGAAGGTTGACGAAAAGATCGACATCTACAGCTACGGTGTGGTTCTATTGGAGCTTCTAACAGGAAAGAGGCCATTAGACCCCGAATTTGGTGAGTCAGTAGACGTCGTGGAATGGATTCGGAGGAAGATTAAGGACAACAAAAGTTTAGAAGCAGCATTAGACCCCGGTGTAGGAAACTGCAAGCATGTTCAAGAAGAGATGCTCTTAGTCCTTAGAATAGCATTGCTTTGCACTGCCAAGCTTCCGAAGGACAGGCCTTCCATGAGGGATGTCATAACGATGCTCGGAGAGGCGAAGCCCCGGCGAAAAAGCAGCAGCAACAACAACGATGCATATGCAGCAGCTAACAAGGACCAGCATGTGTTTAGCACCTCACCTGTACATGGCCTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1015

Amino Acids

109.95

Weight (kDa)

5.9

Isoelectric Point (pI)

31.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 66 1.9e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 74 - 172 1.5e-06 Leucine-rich repeat region
LRR_8 PF13855 167 - 224 2.4e-07 Leucine rich repeat
LRR_14 PF23598 183 - 265 5.3e-07 Leucine-rich repeat region
LRR_14 PF23598 232 - 318 2.9e-10 Leucine-rich repeat region
LRR_14 PF23598 276 - 343 1.8e-07 Leucine-rich repeat region
LRR_14 PF23598 329 - 563 9.2e-11 Leucine-rich repeat region
LRR_8 PF13855 458 - 513 7.9e-07 Leucine rich repeat
LRR_8 PF13855 477 - 537 1.3e-07 Leucine rich repeat
LRR_8 PF13855 526 - 585 3e-07 Leucine rich repeat
Pkinase PF00069 704 - 975 5.8e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 704 - 977 5.9e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28650
fragaria_vesca FvH4_2g20490
malus_domestica MD05G1188700.v1.1 MD10G1176300.v1.1
prunus_persica Prupe.8G215300_v2.0.a1
pyrus_communis pycom10g15220
rosa_chinensis RchiOBHm_Chr6g0286591
rosa_laevigata RLG00000012556
rosa_multiflora Rmu_sc0000560.1_g000003
rosa_roxburghii Rroxscaffold_7G00182160
rosa_rugosa Rorug06G0181300
rosa_samantha Rh6AG294200 Rh6BG297300 Rh6CG297700 Rh6DG289600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2754
AccB1I GGYRCC 1 cut(s) 845
AccB7I CCANNNNNTGG 1 cut(s) 1775
AccI GTMKAC 1 cut(s) 2748
AccIII TCCGGA 1 cut(s) 1253
AciI CCGC 4 cut(s) 246, 618, 1390, 2549
AclWI GGATC 7 cut(s) 116, 486, 519, 649, 879, 1934, 2617
AcoI YGGCCR 2 cut(s) 72, 2042
AcsI RAATTY 5 cut(s) 828, 928, 1702, 2323, 2732
AcuI CTGAAG 4 cut(s) 737, 764, 944, 1277
AcvI CACGTG 1 cut(s) 1209
AcyI GRCGYC 2 cut(s) 846, 2751
AdeI CACNNNGTG 1 cut(s) 1131
AfaI GTAC 6 cut(s) 665, 1991, 2086, 2150, 2423, 3030
AfiI CCNNNNNNNGG 7 cut(s) 161, 257, 1775, 2636, 2735, 2819, 3032
AhdI GACNNNNNGTC 1 cut(s) 2503
AjnI CCWGG 2 cut(s) 1491, 2094
AjuI GAANNNNNNNTTGG 2 cut(s) 1448, 1480
AleI CACNNNNGTG 1 cut(s) 2180
Alw21I GWGCWC 3 cut(s) 169, 1893, 1969
Alw44I GTGCAC 1 cut(s) 1889
AlwI GGATC 7 cut(s) 116, 486, 519, 649, 879, 1934, 2617
Ama87I CYCGRG 1 cut(s) 1798
Aor13HI TCCGGA 1 cut(s) 1253
AoxI GGCC 8 cut(s) 72, 112, 247, 1449, 2042, 2717, 2903, 3034
ApaLI GTGCAC 1 cut(s) 1889
ApeKI GCWGC 9 cut(s) 65, 303, 934, 2358, 2801, 2961, 2964, 2987, 2990
ApoI RAATTY 5 cut(s) 828, 928, 1702, 2323, 2732
ArsI GACNNNNNNTTYG 2 cut(s) 2717, 2749
Asp700I GAANNNNTTC 2 cut(s) 527, 2763
AspLEI GCGC 1 cut(s) 848
AspS9I GGNCC 5 cut(s) 248, 1040, 1184, 1760, 3001
AsuC2I CCSGG 5 cut(s) 251, 607, 2140, 2814, 2950
AsuHPI GGTGA 9 cut(s) 404, 764, 1124, 1141, 1707, 1930, 2468, 2750, 3015
AsuII TTCGAA 2 cut(s) 1231, 2022
AvaI CYCGRG 1 cut(s) 1798
AvaII GGWCC 4 cut(s) 1040, 1184, 1760, 3001
AxyI CCTNAGG 1 cut(s) 474
BaeGI GKGCMC 2 cut(s) 210, 1893
BalI TGGCCA 2 cut(s) 74, 2044
BanI GGYRCC 1 cut(s) 845
BanII GRGCYC 2 cut(s) 169, 539
BarI GAAGNNNNNNTAC 1 cut(s) 3021
BbrPI CACGTG 1 cut(s) 1209
Bbv12I GWGCWC 3 cut(s) 169, 1893, 1969
BbvCI CCTCAGC 1 cut(s) 242
BbvI GCAGC 9 cut(s) 77, 290, 946, 2370, 2813, 2973, 2976, 2999, 3002
BccI CCATC 7 cut(s) 344, 1129, 1441, 1556, 1908, 2050, 2566
BcgI CGANNNNNNTGC 6 cut(s) 71, 81, 105, 115, 2966, 3000
BciT130I CCWGG 2 cut(s) 1493, 2096
BciVI GTATCC 2 cut(s) 265, 2633
BclI TGATCA 1 cut(s) 2331
BcnI CCSGG 5 cut(s) 251, 607, 2140, 2814, 2950
BfaI CTAG 4 cut(s) 1050, 1754, 2243, 3043
BfmI CTRYAG 2 cut(s) 304, 2674
BfoI RGCGCY 1 cut(s) 849
BfuI GTATCC 2 cut(s) 265, 2633
BmcAI AGTACT 1 cut(s) 2086
Bme1390I CCNGG 7 cut(s) 251, 607, 1493, 2096, 2140, 2814, 2950
Bme18I GGWCC 4 cut(s) 1040, 1184, 1760, 3001
BmeRI GACNNNNNGTC 1 cut(s) 2503
BmeT110I CYCGRG 1 cut(s) 1798
BmgT120I GGNCC 5 cut(s) 248, 1040, 1184, 1760, 3001
BmiI GGNNCC 4 cut(s) 847, 1546, 1761, 2134
BmrFI CCNGG 7 cut(s) 251, 607, 1493, 2096, 2140, 2814, 2950
BmrI ACTGGG 1 cut(s) 1259
BmsI GCATC 7 cut(s) 1632, 1880, 2287, 2515, 2838, 2919, 2968
BmuI ACTGGG 1 cut(s) 1259
BplI GAGNNNNNCTC 2 cut(s) 623, 655
BpmI CTGGAG 2 cut(s) 441, 521
Bpu10I CCTNAGC 3 cut(s) 242, 2273, 2362
Bpu14I TTCGAA 2 cut(s) 1231, 2022
BpuEI CTTGAG 4 cut(s) 557, 809, 1034, 1304
BpuMI CCSGG 5 cut(s) 251, 607, 2140, 2814, 2950
BsaAI YACGTR 2 cut(s) 211, 1209
BsaBI GATNNNNATC 2 cut(s) 1925, 2669
BsaHI GRCGYC 2 cut(s) 846, 2751
BsaI GGTCTC 1 cut(s) 2197
BsaJI CCNNGG 8 cut(s) 401, 606, 658, 1722, 2045, 2139, 2812, 2948
BsaWI WCCGGW 3 cut(s) 154, 1109, 1253
Bsc4I CCNNNNNNNGG 7 cut(s) 161, 257, 1775, 2636, 2735, 2819, 3032
Bse118I RCCGGY 1 cut(s) 2206
Bse1I ACTGG 7 cut(s) 149, 1265, 1330, 1690, 1862, 2230, 2483
Bse21I CCTNAGG 1 cut(s) 474
Bse3DI GCAATG 7 cut(s) 60, 493, 1073, 1560, 1822, 2038, 2870
Bse8I GATNNNNATC 2 cut(s) 1925, 2669
BseAI TCCGGA 1 cut(s) 1253
BseBI CCWGG 2 cut(s) 1493, 2096
BseDI CCNNGG 8 cut(s) 401, 606, 658, 1722, 2045, 2139, 2812, 2948
BseJI GATNNNNATC 2 cut(s) 1925, 2669
BseLI CCNNNNNNNGG 7 cut(s) 161, 257, 1775, 2636, 2735, 2819, 3032
BseMI GCAATG 7 cut(s) 60, 493, 1073, 1560, 1822, 2038, 2870
BseNI ACTGG 7 cut(s) 149, 1265, 1330, 1690, 1862, 2230, 2483
BseRI GAGGAG 1 cut(s) 307
BseSI GKGCMC 2 cut(s) 210, 1893
BseXI GCAGC 9 cut(s) 77, 290, 946, 2370, 2813, 2973, 2976, 2999, 3002
BseYI CCCAGC 2 cut(s) 904, 1552
BsgI GTGCAG 2 cut(s) 75, 1410
BshFI GGCC 8 cut(s) 74, 114, 249, 1451, 2044, 2719, 2905, 3036
BshNI GGYRCC 1 cut(s) 845
BsiHKAI GWGCWC 3 cut(s) 169, 1893, 1969
BsiHKCI CYCGRG 1 cut(s) 1798
BslFI GGGAC 1 cut(s) 1751
BslI CCNNNNNNNGG 7 cut(s) 161, 257, 1775, 2636, 2735, 2819, 3032
BsmBI CGTCTC 2 cut(s) 278, 1721
BsmFI GGGAC 1 cut(s) 1751
BsmI GAATGC 2 cut(s) 1239, 2060
BsnI GGCC 8 cut(s) 74, 114, 249, 1451, 2044, 2719, 2905, 3036
Bso31I GGTCTC 1 cut(s) 2197
BsoBI CYCGRG 1 cut(s) 1798
Bsp119I TTCGAA 2 cut(s) 1231, 2022
Bsp1286I GDGCHC 5 cut(s) 169, 210, 539, 1893, 1969
Bsp13I TCCGGA 1 cut(s) 1253
Bsp1407I TGTACA 2 cut(s) 2148, 3028
Bsp19I CCATGG 2 cut(s) 401, 2045
BspACI CCGC 4 cut(s) 246, 618, 1390, 2549
BspANI GGCC 8 cut(s) 74, 114, 249, 1451, 2044, 2719, 2905, 3036
BspEI TCCGGA 1 cut(s) 1253
BspLI GGNNCC 4 cut(s) 847, 1546, 1761, 2134
BspMAI CTGCAG 1 cut(s) 308
BspPI GGATC 7 cut(s) 116, 486, 519, 649, 879, 1934, 2617
BspT104I TTCGAA 2 cut(s) 1231, 2022
BspT107I GGYRCC 1 cut(s) 845
BspTNI GGTCTC 1 cut(s) 2197
BsrDI GCAATG 7 cut(s) 60, 493, 1073, 1560, 1822, 2038, 2870
BsrFI RCCGGY 1 cut(s) 2206
BsrGI TGTACA 2 cut(s) 2148, 3028
BsrI ACTGG 7 cut(s) 149, 1265, 1330, 1690, 1862, 2230, 2483
BssAI RCCGGY 1 cut(s) 2206
BssECI CCNNGG 8 cut(s) 401, 606, 658, 1722, 2045, 2139, 2812, 2948
BssNI GRCGYC 2 cut(s) 846, 2751
BssT1I CCWWGG 4 cut(s) 401, 658, 1722, 2045
Bst2UI CCWGG 2 cut(s) 1493, 2096
Bst4CI ACNGT 7 cut(s) 599, 796, 2018, 2179, 2593, 2618, 2684
Bst6I CTCTTC 2 cut(s) 1169, 2838
BstACI GRCGYC 2 cut(s) 846, 2751
BstAPI GCANNNNNTGC 2 cut(s) 1562, 2546
BstAUI TGTACA 2 cut(s) 2148, 3028
BstBAI YACGTR 2 cut(s) 211, 1209
BstBI TTCGAA 2 cut(s) 1231, 2022
BstDSI CCRYGG 2 cut(s) 401, 2045
BstH2I RGCGCY 1 cut(s) 849
BstHHI GCGC 1 cut(s) 848
BstNI CCWGG 2 cut(s) 1493, 2096
BstNSI RCATGY 3 cut(s) 1855, 2837, 3011
BstSCI CCNGG 7 cut(s) 249, 605, 1491, 2094, 2138, 2812, 2948
BstSFI CTRYAG 2 cut(s) 304, 2674
BstSLI GKGCMC 2 cut(s) 210, 1893
BstV1I GCAGC 9 cut(s) 77, 290, 946, 2370, 2813, 2973, 2976, 2999, 3002
BstX2I RGATCY 4 cut(s) 478, 511, 871, 1926
BstXI CCANNNNNNTGG 3 cut(s) 499, 1080, 2072
BstYI RGATCY 4 cut(s) 478, 511, 871, 1926
Bsu36I CCTNAGG 1 cut(s) 474
BsuI GTATCC 2 cut(s) 265, 2633
BsuRI GGCC 8 cut(s) 74, 114, 249, 1451, 2044, 2719, 2905, 3036
BtgI CCRYGG 2 cut(s) 401, 2045
BtsI GCAGTG 8 cut(s) 92, 169, 313, 323, 1085, 1840, 2239, 2880
CfoI GCGC 1 cut(s) 848
Cfr10I RCCGGY 1 cut(s) 2206
Cfr13I GGNCC 5 cut(s) 248, 1040, 1184, 1760, 3001
CseI GACGC 2 cut(s) 1738, 2546
Csp6I GTAC 6 cut(s) 664, 1990, 2085, 2149, 2422, 3029
CspCI CAANNNNNGTGG 4 cut(s) 2125, 2160, 2453, 2488
CviQI GTAC 6 cut(s) 664, 1990, 2085, 2149, 2422, 3029
DinI GGCGCC 1 cut(s) 847
DraIII CACNNNGTG 1 cut(s) 1131
DriI GACNNNNNGTC 1 cut(s) 2503
EaeI YGGCCR 2 cut(s) 72, 2042
Eam1104I CTCTTC 2 cut(s) 1169, 2838
Eam1105I GACNNNNNGTC 1 cut(s) 2503
EarI CTCTTC 2 cut(s) 1169, 2838
Ecl136II GAGCTC 1 cut(s) 167
Eco130I CCWWGG 4 cut(s) 401, 658, 1722, 2045
Eco147I AGGCCT 2 cut(s) 114, 2905
Eco24I GRGCYC 2 cut(s) 169, 539
Eco31I GGTCTC 1 cut(s) 2197
Eco32I GATATC 1 cut(s) 2163
Eco47I GGWCC 4 cut(s) 1040, 1184, 1760, 3001
Eco53kI GAGCTC 1 cut(s) 167
Eco57I CTGAAG 4 cut(s) 737, 764, 944, 1277
Eco72I CACGTG 1 cut(s) 1209
Eco81I CCTNAGG 1 cut(s) 474
Eco88I CYCGRG 1 cut(s) 1798
EcoICRI GAGCTC 1 cut(s) 167
EcoO109I RGGNCCY 2 cut(s) 1040, 1760
EcoRII CCWGG 2 cut(s) 1491, 2094
EcoRV GATATC 1 cut(s) 2163
EcoT14I CCWWGG 4 cut(s) 401, 658, 1722, 2045
EcoT22I ATGCAT 1 cut(s) 2983
EcoT38I GRGCYC 2 cut(s) 169, 539
EgeI GGCGCC 1 cut(s) 847
EheI GGCGCC 1 cut(s) 847
ErhI CCWWGG 4 cut(s) 401, 658, 1722, 2045
Esp3I CGTCTC 2 cut(s) 278, 1721
FalI AAGNNNNNCTT 4 cut(s) 271, 303, 1549, 1581
FaqI GGGAC 1 cut(s) 1751
FauI CCCGC 1 cut(s) 625
FauNDI CATATG 1 cut(s) 2983
FbaI TGATCA 1 cut(s) 2331
FblI GTMKAC 1 cut(s) 2748
FriOI GRGCYC 2 cut(s) 169, 539
FspBI CTAG 4 cut(s) 1050, 1754, 2243, 3043
GlaI GCGC 1 cut(s) 847
GsaI CCCAGC 2 cut(s) 908, 1556
GsuI CTGGAG 2 cut(s) 441, 521
HaeII RGCGCY 1 cut(s) 849
HaeIII GGCC 8 cut(s) 74, 114, 249, 1451, 2044, 2719, 2905, 3036
HgaI GACGC 2 cut(s) 1738, 2546
HhaI GCGC 1 cut(s) 848
Hin1I GRCGYC 2 cut(s) 846, 2751
Hin6I GCGC 1 cut(s) 846
HinP1I GCGC 1 cut(s) 846
HincII GTYRAC 1 cut(s) 2656
HindII GTYRAC 1 cut(s) 2656
HindIII AAGCTT 5 cut(s) 218, 1280, 1432, 1585, 2888
HphI GGTGA 9 cut(s) 404, 764, 1124, 1141, 1707, 1930, 2468, 2750, 3015
Hpy166II GTNNAC 4 cut(s) 1891, 2149, 2656, 2749
Hpy8I GTNNAC 4 cut(s) 1891, 2149, 2656, 2749
Hpy99I CGWCG 2 cut(s) 2540, 2756
HpyAV CCTTC 9 cut(s) 671, 815, 1419, 1512, 2101, 2262, 2644, 2890, 2916
HpyCH4III ACNGT 7 cut(s) 599, 796, 2018, 2179, 2593, 2618, 2684
HpyCH4IV ACGT 4 cut(s) 210, 271, 1208, 2751
HpySE526I ACGT 4 cut(s) 210, 271, 1208, 2751
Hsp92I GRCGYC 2 cut(s) 846, 2751
HspAI GCGC 1 cut(s) 846
KasI GGCGCC 1 cut(s) 845
Kpn2I TCCGGA 1 cut(s) 1253
Ksp22I TGATCA 1 cut(s) 2331
LmnI GCTCC 4 cut(s) 164, 919, 2132, 2698
Lsp1109I GCAGC 9 cut(s) 77, 290, 946, 2370, 2813, 2973, 2976, 2999, 3002
LweI GCATC 7 cut(s) 1632, 1880, 2287, 2515, 2838, 2919, 2968
MaeI CTAG 4 cut(s) 1050, 1754, 2243, 3043
MaeII ACGT 4 cut(s) 210, 271, 1208, 2751
MaeIII GTNAC 3 cut(s) 2234, 2474, 2618
MfeI CAATTG 4 cut(s) 20, 175, 1607, 1632
MflI RGATCY 4 cut(s) 478, 511, 871, 1926
MhlI GDGCHC 5 cut(s) 169, 210, 539, 1893, 1969
MlsI TGGCCA 2 cut(s) 74, 2044
MluNI TGGCCA 2 cut(s) 74, 2044
Mly113I GGCGCC 1 cut(s) 846
MlyI GAGTC 6 cut(s) 382, 499, 1006, 1818, 1944, 2750
MmeI TCCRAC 6 cut(s) 292, 1065, 1122, 1695, 1927, 2416
Mox20I TGGCCA 2 cut(s) 74, 2044
Mph1103I ATGCAT 1 cut(s) 2983
MroI TCCGGA 1 cut(s) 1253
MroXI GAANNNNTTC 2 cut(s) 527, 2763
MscI TGGCCA 2 cut(s) 74, 2044
MseI TTAA 3 cut(s) 1004, 2190, 2778
MslI CAYNNNNRTG 4 cut(s) 1078, 1884, 2180, 2927
Msp20I TGGCCA 2 cut(s) 74, 2044
MspA1I CMGCKG 7 cut(s) 68, 246, 620, 764, 836, 908, 961
MspR9I CCNGG 7 cut(s) 251, 607, 1493, 2096, 2140, 2814, 2950
MunI CAATTG 4 cut(s) 20, 175, 1607, 1632
Mva1269I GAATGC 2 cut(s) 1239, 2060
MvaI CCWGG 2 cut(s) 1493, 2096
NarI GGCGCC 1 cut(s) 846
NciI CCSGG 5 cut(s) 251, 607, 2140, 2814, 2950
NcoI CCATGG 2 cut(s) 401, 2045
NdeI CATATG 1 cut(s) 2983
NlaIV GGNNCC 4 cut(s) 847, 1546, 1761, 2134
NmeAIII GCCGAG 1 cut(s) 462
NmuCI GTSAC 2 cut(s) 2234, 2474
NsiI ATGCAT 1 cut(s) 2983
NspI RCATGY 3 cut(s) 1855, 2837, 3011
NspV TTCGAA 2 cut(s) 1231, 2022
OliI CACNNNNGTG 1 cut(s) 2180
PaeI GCATGC 1 cut(s) 1855
PceI AGGCCT 2 cut(s) 114, 2905
PcsI WCGNNNNNNNCGW 1 cut(s) 2664
PctI GAATGC 2 cut(s) 1239, 2060
PdmI GAANNNNTTC 2 cut(s) 527, 2763
PflFI GACNNNGTC 1 cut(s) 2593
PflMI CCANNNNNTGG 1 cut(s) 1775
PfoI TCCNGGA 1 cut(s) 1491
PleI GAGTC 6 cut(s) 381, 499, 1005, 1818, 1944, 2749
PluTI GGCGCC 1 cut(s) 849
PmaCI CACGTG 1 cut(s) 1209
PmlI CACGTG 1 cut(s) 1209
PpsI GAGTC 6 cut(s) 381, 499, 1005, 1818, 1944, 2749
Ppu21I YACGTR 2 cut(s) 211, 1209
PpuMI RGGWCCY 2 cut(s) 1040, 1760
Psp124BI GAGCTC 1 cut(s) 169
Psp5II RGGWCCY 2 cut(s) 1040, 1760
Psp6I CCWGG 2 cut(s) 1491, 2094
PspCI CACGTG 1 cut(s) 1209
PspFI CCCAGC 2 cut(s) 904, 1552
PspGI CCWGG 2 cut(s) 1491, 2094
PspN4I GGNNCC 4 cut(s) 847, 1546, 1761, 2134
PspPI GGNCC 5 cut(s) 248, 1040, 1184, 1760, 3001
PspPPI RGGWCCY 2 cut(s) 1040, 1760
PstI CTGCAG 1 cut(s) 308
PsuI RGATCY 4 cut(s) 478, 511, 871, 1926
PsyI GACNNNGTC 1 cut(s) 2593
PvuII CAGCTG 5 cut(s) 68, 764, 836, 908, 961
RsaI GTAC 6 cut(s) 665, 1991, 2086, 2150, 2423, 3030
RsaNI GTAC 6 cut(s) 664, 1990, 2085, 2149, 2422, 3029
RseI CAYNNNNRTG 4 cut(s) 1078, 1884, 2180, 2927
SacI GAGCTC 1 cut(s) 169
SaqAI TTAA 3 cut(s) 1004, 2190, 2778
Sau96I GGNCC 5 cut(s) 248, 1040, 1184, 1760, 3001
ScaI AGTACT 1 cut(s) 2086
SchI GAGTC 6 cut(s) 382, 499, 1006, 1818, 1944, 2750
ScrFI CCNGG 7 cut(s) 251, 607, 1493, 2096, 2140, 2814, 2950
SduI GDGCHC 5 cut(s) 169, 210, 539, 1893, 1969
SfaNI GCATC 7 cut(s) 1632, 1880, 2287, 2515, 2838, 2919, 2968
SfcI CTRYAG 2 cut(s) 304, 2674
SfoI GGCGCC 1 cut(s) 847
SfuI TTCGAA 2 cut(s) 1231, 2022
SinI GGWCC 4 cut(s) 1040, 1184, 1760, 3001
SmiMI CAYNNNNRTG 4 cut(s) 1078, 1884, 2180, 2927
SmlI CTYRAG 4 cut(s) 572, 788, 1013, 1283
SmoI CTYRAG 4 cut(s) 572, 788, 1013, 1283
SphI GCATGC 1 cut(s) 1855
SseBI AGGCCT 2 cut(s) 114, 2905
SsiI CCGC 4 cut(s) 246, 618, 1390, 2549
SspDI GGCGCC 1 cut(s) 845
SspMI CTAG 4 cut(s) 1050, 1754, 2243, 3043
SstI GAGCTC 1 cut(s) 169
StuI AGGCCT 2 cut(s) 114, 2905
StyD4I CCNGG 7 cut(s) 249, 605, 1491, 2094, 2138, 2812, 2948
StyI CCWWGG 4 cut(s) 401, 658, 1722, 2045
TaaI ACNGT 7 cut(s) 599, 796, 2018, 2179, 2593, 2618, 2684
TaiI ACGT 4 cut(s) 213, 274, 1211, 2754
TatI WGTACW 3 cut(s) 2084, 2148, 3028
TauI GCSGC 1 cut(s) 249
Tru1I TTAA 3 cut(s) 1004, 2190, 2778
Tru9I TTAA 3 cut(s) 1004, 2190, 2778
TseFI GTSAC 2 cut(s) 2234, 2474
TseI GCWGC 9 cut(s) 65, 303, 934, 2358, 2801, 2961, 2964, 2987, 2990
Tsp45I GTSAC 2 cut(s) 2234, 2474
TspDTI ATGAA 6 cut(s) 42, 119, 251, 1360, 1476, 1557
TspGWI ACGGA 4 cut(s) 446, 1629, 1836, 2581
Tth111I GACNNNGTC 1 cut(s) 2593
Van91I CCANNNNNTGG 1 cut(s) 1775
VneI GTGCAC 1 cut(s) 1889
VpaK11BI GGWCC 4 cut(s) 1040, 1184, 1760, 3001
XapI RAATTY 5 cut(s) 828, 928, 1702, 2323, 2732
XceI RCATGY 3 cut(s) 1855, 2837, 3011
XmiI GTMKAC 1 cut(s) 2748
XmnI GAANNNNTTC 2 cut(s) 527, 2763
XspI CTAG 4 cut(s) 1050, 1754, 2243, 3043
ZraI GACGTC 1 cut(s) 2752
ZrmI AGTACT 1 cut(s) 2086
Zsp2I ATGCAT 1 cut(s) 2983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.